CN108029952A - It is a kind of to be used to cultivate the culture medium of ganoderma lucidum mycelium and manufactured ganoderma lucidum mycelium - Google Patents
It is a kind of to be used to cultivate the culture medium of ganoderma lucidum mycelium and manufactured ganoderma lucidum mycelium Download PDFInfo
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- CN108029952A CN108029952A CN201711257975.2A CN201711257975A CN108029952A CN 108029952 A CN108029952 A CN 108029952A CN 201711257975 A CN201711257975 A CN 201711257975A CN 108029952 A CN108029952 A CN 108029952A
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- Prior art keywords
- ganoderma lucidum
- mycelium
- parts
- culture medium
- lucidum mycelium
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L7/00—Cereal-derived products; Malt products; Preparation or treatment thereof
- A23L7/10—Cereal-derived products
- A23L7/104—Fermentation of farinaceous cereal or cereal material; Addition of enzymes or microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L31/00—Edible extracts or preparations of fungi; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Nutrition Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Biotechnology (AREA)
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- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Medicinal Chemistry (AREA)
- Botany (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Tropical Medicine & Parasitology (AREA)
- Medicines Containing Plant Substances (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The present invention relates to ganoderma lucidum immune health health food and ganoderma lucidum food additives, and the invention further relates to the culture medium for being used to prepare above-mentioned food and food additives, the culture medium is mainly prepared by the raw material of following parts by weight：81~210 parts of 30~80 parts of corn, 20~40 parts of wheat, 2~10 parts of soybean, 2~10 parts of millet and water；Wherein, the corn, wheat, soybean and millet are unbroken, are complete grain.Culture medium of the present invention meets in ganoderma lucidum growth course the needs of to nutrition and gas permeability, can make ganoderma lucidum health, Fast Growth, the content of various nutriments is high in gained ganoderma lucidum mycelium, full of nutrition.
Description
Technical field
The present invention relates to fungi field, in particular to a kind of culture medium for cultivating ganoderma lucidum mycelium and is made
Ganoderma lucidum mycelium.
Background technology
Ganoderma lucidum is also known as polyporus lucidus, refreshing sesame, sesame grass, celestial grass, seocho, is the complete stool of Polyporaceae plant red sesame or purple sesame.Spirit
Sesame is Basidiomycetes Polyporaceae (Polyporaceae) Ganoderma (Ganodelma) fungi red sesame (G.lucidum.karst)
With the general name of purple sesame (G.japonicrnLloyd), quilt《This warp》Referred to as top grade.Since ancient times, ganoderma lucidum be regarded as it is lucky,
Rich and honour, fine and long-lived symbol, has the title of " celestial grass " and " seocho ", the direct-view for a long time of chinese tradition medicine is nourishing
Salubrity, the precious Chinese herbal medicine for consolidating righting.
Modern scientific research shows that the nutritional ingredient of ganoderma lucidum includes：Carbon, hydrogen, oxygen, nitrogen, sulphur, phosphorus, potassium, magnesium, calcium, iron, copper,
The trace element such as manganese and zinc and ganoderma lucidum polysaccharide, ganodenic acid and nucleotide etc..These nutritional ingredients are directly entered human body
All warps of liver, kidney, courage, spleen, lung, and there is extensive pharmacological action, and toxicity is extremely low, such as have found ganoderma lucidum have it is antitumor,
Immunological regulation, tranquilizing and allaying excitement, cardiac stimulant and resist myocardial ischemia, adjust blood fat, relieving asthma, liver protection, hypoglycemic, anti anoxia and remove freely
Base and the effect of anti-aging.Long-term use of ganoderma lucidum, can strengthen the body resistance to consolidate the constitution effect, promote longevity.Therefore, ganoderma lucidum becomes current
Research and develop one of most medicinal fungi.
At present, the utilization of ganoderma lucidum focuses primarily upon fructification and conidia powder.Ganoderma lucidum product on the market is mostly that ganoderma lucidum is real
Body or lucidum spore powder.But the quality of ganoderma lucidum fruitbody is easily influenced by many factors, such as the timber kind of culture,
The temperature of growth, humidity, the time of harvesting, the factor such as mode of drying influence, and cause the spirit produced using fructification as raw material
The consequence of sesame unstable product quality.And although the content of each nutriment is high in Reishi sporule, its spore wall thickness, without broken
Wall processing, nutritional ingredient are difficult to effectively be absorbed by human body, and the complex process of ganoderma lucidum wall-broken processing so that Reishi sporule powder
Cost is excessive.
Compared with fructification and conidia powder, ganoderma lucidum mycelium have cultivation cycle is short, stay in grade, processing easy to process and
The advantages such as cost performance height, have great social economic value.But lack the special culture for being adapted to ganoderma lucidum mycelium growth at present
Base and cultural method, existing culture medium and cultural method not only each nutriment in low output but also culture gained mycelium
Content is low, quality is not high.
Therefore, this area is urgently improved existing culture medium and cultural method, to improve ganoderma lucidum mycelium
Yield, while lift the quality of ganoderma lucidum mycelium.
In view of this, it is special to propose the present invention.
The content of the invention
The first object of the present invention is to provide a kind of culture medium for being used to cultivate ganoderma lucidum mycelium, the culture medium satisfaction
To the demand of nutrition and gas permeability in ganoderma lucidum growth course, ganoderma lucidum health, Fast Growth can be made, it is each in gained ganoderma lucidum mycelium
The content of kind nutriment is high, full of nutrition.
The second object of the present invention is to provide a kind of method for preparing ganoderma lucidum mycelium, the method have yield it is high,
The advantages of nutrition content enriches in Ganoderma lucidum mycelium, quality is high.
The third object of the present invention is to provide the ganoderma lucidum mycelium according to made of preceding method, Ganoderma Lucidum of the present invention
Filament is enriched with nutrition content, and quality is good, the advantages that being easily absorbed by the human body.
To realize the above-mentioned purpose of the present invention, spy uses following technical scheme：
It is a kind of to be used to cultivating the culture medium of ganoderma lucidum mycelium, the culture medium mainly prepared by the raw material of following parts by weight and
Into：81~210 parts of 30~80 parts of corn, 20~40 parts of wheat, 2~10 parts of soybean, 2~10 parts of millet and water；Wherein, the jade
Rice, wheat, soybean and millet are unbroken, are complete grain.
Culture medium is the place for the various nutriments that ganoderma lucidum depends on for existence, identical with production environment condition using kind
In the case of, the quality of culture medium will directly affect the quality of ganoderma lucidum.First, culture medium of the present invention is with corn, wheat, big
Beans and millet are prepared for primary raw material.Wherein, the nutritional ingredient of corn is comprehensive, containing protein 8.5%, fat 4.3%, sugar
Class 73.2%, calcium 0.022%, phosphorus 0.021%, iron 0.0016%, also have carrotene, vitamin B1, B2 and niacin with
And the trace element such as sitosterol, lecithin, vitamin E；Wheat starch-containing 53~70%, protein about 11%, carbohydrate 2~7%,
Dextrin 2~10%, fat about 1.6%, crude fibre about 2%；Soybean is containing protein 36.3%, fat 13.4%, carbohydrate
25%th, calcium 0.0037%, phosphorus 0.00571%, iron 0.0011%, also containing carrotene, thiamine and riboflavin etc.；In millet
Containing forming sediment 66.85%~69.76%, protein is up to 11.2%~13.4%, fat 4.5%~5.85%.The present invention by corn,
Cooperation between wheat, soybean and millet, meet ganoderma lucidum mycelium in fast growth process to C sources, N sources, phosphorus, sulphur, calcium,
The requirement of iron, trace element and growth factor, cultivates content height, the mycelium bacterium of each nutriment in the ganoderma lucidum mycelium of gained
Filament is dense, pure white, neat, and condition is good, and quality content is high.
Secondly, the corn, wheat, soybean and millet in culture medium of the present invention are unbroken, are complete grain
Food particle, especially corn and soybean, the particle diameter of grain in itself are more than general grain so that the hole of manufactured culture medium
Gap rate is big, good permeability, can fully ensure demand of the ganoderma lucidum mycelium to oxygen so that ganoderma lucidum mycelium in a short time can
It is enough rapidly to breed, improve mycelial yield.
In some specific embodiments, by weight, the corn is 30,40,50,60,70 or 80 parts；
In some specific embodiments, by weight, the wheat is 20,25,30,35 or 40 parts；
In some specific embodiments, by weight, the soybean is 2,3,4,5,6,7,8,9 or 10 parts；
In some specific embodiments, by weight, the millet is 2,3,4,5,6,7,8,9 or 10 parts；
In some specific embodiments, by weight, the water is 81,90,100,110,120,130,140,
150th, 160,170,180,190,200 or 210 parts.
In some specific embodiments, the culture medium is prepared in the following manner：By the raw material in proportion
It is uniformly mixed, more than 121 DEG C, under conditions of 1~1.5 atmospheric pressure, sterilize 2~4h；After sterilizing, be cooled to 30 DEG C with
Under, to obtain the final product.
In some specific embodiments, the atmospheric pressure is 1,1.1,1.2,1.3,1.4 or 1.5 atmospheric pressure；
In some specific embodiments, the sterilization time is 2,2.5,3,3.5 or 4h；
In some specific embodiments, the culture medium is cooled to 30,29,28,27,26,25,24,23,22,21
Or 20 DEG C；
The invention further relates to a kind of method for preparing ganoderma lucidum mycelium, the described method includes：
(1) in foregoing inoculation of medium lucidum strain, inoculum concentration is 1~5g/Kg culture mediums；
(2) ganoderma lucidum is cultivated under conditions of temperature is 23~27 DEG C, relative humidity is 55~79%, during which, first kept away
Optical culture 10~15 days, then illumination cultivation 15~20 days.
The method of the invention is cultivated by matrix of prior culture media, and the matrix is not only mycelial fast fast-growing
It is long that sufficient nutriment is provided, meanwhile, the matrix is prepared with unbroken grain, have high porosity and
Aeration, meets requirement of the mycelium to high oxygen demand in fast growth process.
Secondly, the method for the invention further defines temperature, humidity and the illumination condition of ganoderma lucidum culture, the temperature
Degree, humidity and illumination condition meet the psychological need of ganoderma lucidum mycelium growth, can induce ganoderma lucidum and sprout mycelium, promote mycelia
The propagation of body.
In some specific embodiments, the inoculum concentration is 1,2,3,4 or 5g/Kg culture mediums；
In some specific embodiments, the temperature of the step (2) is 23,24,25,26 or 27 DEG C；
In some specific embodiments, the relative humidity of the step (2) is 55,57,59,61,63,65,67,
69th, 71,73,75,77 or 79%；
In some specific embodiments, in whole mycelial incubation, divulge information 1~2 time daily, it is logical every time
20~35min of wind；Preferably, ventilation 2 times daily, divulge information 30min every time.
In some specific embodiments, in step (1), the inoculum concentration of the lucidum strain is 2g/Kg culture mediums.
In some specific embodiments, in step (2), the temperature is 25 DEG C, relative humidity 60%, lucifuge training
The foster time is 12 days and the illumination cultivation time is 17 days.
In some specific embodiments, the method further includes ganoderma lucidum mycelium the dry and powder together with culture medium
It is broken, up to mycelium finished product.
In some specific embodiments, to avoid the endotoxin contamination mycelium that miscellaneous bacteria secretes, its quality, this hair are influenced
Bright the method is additionally included in before drying and crushing, rejects the ganoderma lucidum mycelium being infected by bacteria.
In some specific embodiments, the method for the invention further include the mycelium finished product that will be obtained after crushing into
One step is processed as the step of ganoderma lucidum mycelium food or ganoderma lucidum mycelium food additives.
It is of the present invention further to promote absorption of the human body to ganoderma lucidum mycelium in some specific embodiments
Method is further included mycelium immersion in a solvent；Separation of solid and liquid afterwards, up to ganoderma lucidum mycelium extracting solution.
In some specific embodiments, the solvent is water or ethanol.
The invention further relates to ganoderma lucidum mycelium, ganoderma lucidum mycelium food or ganoderma lucidum mycelium food according to made of preceding method
Product additive.Not only condition is good for ganoderma lucidum mycelium of the present invention, moreover, ganoderma lucidum polysaccharide and ganodenic acid equal size are above leading to
Cross conventional method and cultivate the ganoderma lucidum mycelium formed, nutritive value is high.
Compared with prior art, beneficial effects of the present invention are：
(1) primary raw material of culture medium of the present invention is corn, wheat, soybean and millet, is arranged in pairs or groups between each component
When disclosure satisfy that and meet ganoderma lucidum mycelium in fast growth process to C sources, N sources, phosphorus, sulphur, calcium, iron, trace element and growth
The requirement of the factor, content height, the mycelium mycelium for cultivating each nutriment in the ganoderma lucidum mycelium of gained are dense, pure white, whole
Together, condition is good, and quality content is high.
(2) corn in culture medium of the present invention, wheat, soybean and millet are unbroken, are complete grain,
Especially corn and soybean, the particle diameter of grain in itself are more than general grain so that and the porosity of manufactured culture medium is big,
Good permeability, can fully ensure demand of the ganoderma lucidum mycelium to oxygen so that ganoderma lucidum mycelium in a short time can be quick
Ground is bred, and improves mycelial yield.
(3) according to the present invention the method prepare ganoderma lucidum mycelium have mycelium mycelium it is dense, it is pure white, neat,
The advantages of condition is good, and ganoderma lucidum polysaccharide and the content of ganodenic acid are high, and quality is good, manufactured ganoderma lucidum mycelium food or Ganoderma Lucidum
Filament food additives have the advantages that nutritive value and cost-effective.
Embodiment
Embodiment of the present invention is described in detail below in conjunction with embodiment, but those skilled in the art will
Understand, the following example is merely to illustrate the present invention, and is not construed as limiting the scope of the invention.It is not specified in embodiment specific
Condition person, the condition suggested according to normal condition or manufacturer carry out.Reagents or instruments used without specified manufacturer, is
The conventional products obtained can be bought by city.
Embodiment 1
The culture medium for cultivating ganoderma lucidum mycelium is prepared in accordance with the following methods：
(1) raw material of following parts by weight is weighed：81 parts of 30 parts of corn, 40 parts of wheat, 2 parts of soybean, 10 parts of millet and water, institute
It is unbroken to state corn, wheat, soybean and millet, is complete grain；
(2) raw material is uniformly mixed, is packed with sack filling machine, compressed, sack is buckled with cotton thread and is tightened, is placed on autoclave
In, under 121 DEG C, 1 atmospheric pressure, when sterilizing 3 is small；
(3) after sterilizing, when culture medium is cooled to 30 DEG C, it is put into inoculating hood and is inoculated with lucidum strain, wherein, it is described
The inoculum concentration of lucidum strain is 1g/Kg culture mediums；
(4) after being inoculated with, packed culture medium is placed in yoke culture, specific condition of culture is：Indoor temperature is constant
At 23 DEG C, relative humidity 55%, ventilation 1 time daily, divulge information 20min every time；The early period of lucifuge culture 10 days, afterwards, illumination training
Support 15 days, intensity of illumination 500lux；
(5) after cultivating, will be rejected by the ganoderma lucidum mycelium of living contaminants；Packed culture medium is dismantled, by mycelium
Crushed with together with culture medium, micro mist crushing is carried out after drying, that is, obtain ganoderma lucidum mycelium micropowder powder.
Embodiment 2
The culture medium for cultivating ganoderma lucidum mycelium is prepared in accordance with the following methods：
(1) raw material of following parts by weight is weighed：120 parts of 50 parts of corn, 30 parts of wheat, 6 parts of soybean, 6 parts of millet and water, institute
It is unbroken to state corn, wheat, soybean and millet, is complete grain；
(2) raw material is uniformly mixed, is packed with sack filling machine, compressed, sack is buckled with cotton thread and is tightened, is placed on autoclave
In, under 121 DEG C, 1.3 atmospheric pressure, when sterilizing 2 is small；
(3) after sterilizing, when culture medium is cooled to 25 DEG C, it is put into inoculating hood and is inoculated with lucidum strain, wherein, it is described
The inoculum concentration of lucidum strain is 3g/Kg culture mediums；
(4) after being inoculated with, packed culture medium is placed in yoke culture, specific condition of culture is：Indoor temperature is constant
At 25 DEG C, relative humidity 65%, ventilation 1 time daily, divulge information 30min every time；The early period of lucifuge culture 12 days, afterwards, illumination training
Support 17 days, intensity of illumination 700lux；
(5) after cultivating, will be rejected by the ganoderma lucidum mycelium of living contaminants；Packed culture medium is dismantled, by mycelium
Crushed with together with matrix, micro mist crushing is carried out after drying, that is, obtain ganoderma lucidum mycelium micropowder powder.
Embodiment 3
The culture medium for cultivating ganoderma lucidum mycelium is prepared in accordance with the following methods：
(1) raw material of following parts by weight is weighed：210 parts of 80 parts of corn, 20 parts of wheat, 10 parts of soybean, 2 parts of millet and water,
The corn, wheat, soybean and millet are unbroken, are complete grain；
(2) raw material is uniformly mixed, is packed with sack filling machine, compressed, sack is buckled with cotton thread and is tightened, is placed on autoclave
In, under 121 DEG C, 1.5 atmospheric pressure, when sterilizing 2 is small；
(3) after sterilizing, when culture medium is cooled to 27 DEG C, it is put into inoculating hood and is inoculated with lucidum strain, wherein, it is described
The inoculum concentration of lucidum strain is 5g/Kg culture mediums；
(4) after being inoculated with, packed culture medium is placed in yoke culture, specific condition of culture is：Indoor temperature is constant
At 27 DEG C, relative humidity 79%, ventilation 2 times daily, divulge information 20min every time；The early period of lucifuge culture 15 days, afterwards, illumination training
Support 17 days, intensity of illumination 700lux；
(5) after cultivating, will be rejected by the ganoderma lucidum mycelium of living contaminants；Packed culture medium is dismantled, by mycelium
Crushed with together with matrix, micro mist crushing is carried out after drying, that is, obtain ganoderma lucidum mycelium micropowder powder.
Embodiment 4
Ganoderma lucidum mycelium is prepared with reference to 2 the method for embodiment, is differed only in, the method is obtaining ganoderma lucidum mycelium
After micropowder powder, the powder is added in ethanol solution, solid-liquid ratio 1g:1ml, when immersion 12 is small, centrifuges, solid-liquid afterwards
Separation, up to ganoderma lucidum mycelium extracting solution.
Comparative example 1
Ganoderma lucidum mycelium is prepared with reference to 2 the method for embodiment, is differed only in, the corn, wheat, soybean and millet
For pulverized powder.
Comparative example 2
Ganoderma lucidum mycelium is prepared with reference to 2 the method for embodiment, is differed only in, the composition of raw material described in step (1)
For：120 parts of 50 parts of corn, 30 parts of buckwheat, 6 parts of oat, 6 parts of rice and water.
Experimental example 1
(1) condition of ganoderma lucidum mycelium prepared by embodiment 1~3 and 1~2 the method for comparative example is observed, and counts spirit
The quality of camphorata mycelium powder, calculates the yield of the above method；
(2) ganoderma lucidum polysaccharide and ganodenic acid in embodiment 1~3 and the ganoderma lucidum mycelium powder of comparative example 1~2 are detected
Content；Specific detection method is as follows：
Using the content of sulfuric acid anthrone method detection ganoderma lucidum polysaccharide：Ganoderma lucidum mycelium powder 2.0g is taken, precise weighing, puts Soxhlet
In extractor, add water 90ml, extracting solution is transferred in 100ml volumetric flasks, is diluted with water to quarter to colourless by heating and refluxing extraction
Degree, shake up, precision draw 10ml, add 95% ethanol 150ml shake up, 4 DEG C placement 12h, take out, centrifugation, abandon reset and add it is water-soluble
Solve and be quantitatively transferred in 50ml volumetric flasks and be diluted to scale.
The detection method of ganodenic acid：Using oleanolic acid as standard items, standard curve is drawn；Weigh erinaceus mycelium powder
2.0g, adds 30ml chloroform refluxing extraction 2h, and residue continues refluxing extraction after filtering, is repeated 3 times, merging filtrate is simultaneously settled to
100mL.Precision pipettes 1.0mL and is filled in tool in rub oral examination tube, and heating water bath flings to solvent, then is separately added into 5% vanillas of 0.3mL
Aldehyde glacial acetic acid solution, 1.0mL perchloric acid, sealing.25min is heated in 70 DEG C of constant temperature water baths, frozen water cools down immediately after taking-up, adds
Glacial acetic acid 10mL, shakes up, and absorbance is measured at 550nm, calculates ganodenic acid content.
The specific testing result of step (1) and step (2) is referring to table 1.According to testing result shown in table 1, the present invention
Mycelium prepared by 1~3 the method for embodiment is either in condition, yield still in ganoderma lucidum polysaccharide and the content of ganodenic acid
On be significantly better than that comparative example 1~2.
The condition of 1 ganoderma lucidum mycelium of table, yield, the content of ganoderma lucidum polysaccharide and ganodenic acid
Finally it should be noted that：The above embodiments are only used to illustrate the technical solution of the present invention., rather than its limitations；To the greatest extent
Pipe is described in detail the present invention with reference to foregoing embodiments, but it will be understood by those of ordinary skill in the art that：Its
It can still modify to the technical solution described in foregoing embodiments, either to which part or all technical characteristic
Carry out equivalent substitution；And these modifications or replacement, the essence of appropriate technical solution is departed from various embodiments of the present invention skill
The scope of art scheme.
Claims (10)
1. a kind of culture medium for being used to cultivate ganoderma lucidum mycelium, it is characterised in that the culture medium is mainly by following parts by weight
Raw material is prepared：81~210 parts of 30~80 parts of corn, 20~40 parts of wheat, 2~10 parts of soybean, 2~10 parts of millet and water；
Wherein, the corn, wheat, soybean and millet are unbroken, are complete grain.
2. culture medium according to claim 1, it is characterised in that the culture medium is prepared in the following manner：By institute
State raw material to mix in proportion, more than 121 DEG C, under conditions of 1~1.5 atmospheric pressure, sterilize 2~4h；It is cold after sterilizing
But to less than 30 DEG C, to obtain the final product.
A kind of 3. method for preparing ganoderma lucidum mycelium, it is characterised in that the described method includes：
(1) in the inoculation of medium lucidum strain described in claim 1 or 2, inoculum concentration is 1~5g/Kg culture mediums；
(2) ganoderma lucidum is cultivated under conditions of temperature is 23~27 DEG C, relative humidity is 55~79%, during which, first lucifuge training
Support 10~15 days, then illumination cultivation 15~20 days.
4. according to the method described in claim 3, it is characterized in that, in whole mycelial incubation, divulge information 1 daily~
2 times, divulge information 20~35min every time；Preferably, ventilation 2 times daily, divulge information 30min every time.
5. according to the method described in claim 3, it is characterized in that, in step (1), the inoculum concentration of the lucidum strain is 2g/
Kg culture mediums；With, or
In step (2), the temperature is 25 DEG C, relative humidity 60%, and lucifuge incubation time is 12 days and illumination cultivation time
For 17 days.
6. according to claim 3~5 any one of them method, it is characterised in that the method is further included ganoderma lucidum mycelium
The dry and crushing with together with culture medium, up to mycelium finished product.
7. according to the method described in claim 6, it is characterized in that, the method is additionally included in before drying and crushing, rejecting is
By the ganoderma lucidum mycelium of living contaminants.
8. according to the method described in claim 6, it is characterized in that, the method further include the mycelium that will be obtained after crushing into
Product are further processed into the step of ganoderma lucidum mycelium food or ganoderma lucidum mycelium food additives.
9. according to the method described in claim 6, it is characterized in that, the method is further included mycelium immersion in a solvent；
Separation of solid and liquid afterwards, up to ganoderma lucidum mycelium extracting solution；Preferably, the solvent is water or ethanol.
10. according to ganoderma lucidum mycelium or ganoderma lucidum mycelium food or ganoderma lucidum made of claim 3~9 any one of them method
Mycelium food additives.
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| CN109892169A (en) * | 2019-03-28 | 2019-06-18 | 上海市农业科学院 | A kind of germination method of purple sesame basidiospore germination culture medium and purple sesame basidiospore |
| CN110777081A (en) * | 2019-11-26 | 2020-02-11 | 陈晨 | Production method of high-activity small-molecule composite edible ganoderma lucidum powder |
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| CN110777081A (en) * | 2019-11-26 | 2020-02-11 | 陈晨 | Production method of high-activity small-molecule composite edible ganoderma lucidum powder |
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Application publication date: 20180515 |