CN107927764A - A kind of health food containing sea cucumber extract and earthworm albumen powder and preparation method thereof - Google Patents
A kind of health food containing sea cucumber extract and earthworm albumen powder and preparation method thereof Download PDFInfo
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- CN107927764A CN107927764A CN201711159933.5A CN201711159933A CN107927764A CN 107927764 A CN107927764 A CN 107927764A CN 201711159933 A CN201711159933 A CN 201711159933A CN 107927764 A CN107927764 A CN 107927764A
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- sea cucumber
- earthworm
- temperature
- albumen powder
- cucumber extract
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L17/00—Food-from-the-sea products; Fish products; Fish meal; Fish-egg substitutes; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L17/00—Food-from-the-sea products; Fish products; Fish meal; Fish-egg substitutes; Preparation or treatment thereof
- A23L17/20—Fish extracts
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Polymers & Plastics (AREA)
- Food Science & Technology (AREA)
- Nutrition Science (AREA)
- Health & Medical Sciences (AREA)
- Mycology (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
A kind of health food containing sea cucumber extract and earthworm albumen powder and preparation method thereof, the present invention relates to a kind of health food and preparation method thereof.The invention solves existing health products all with the addition of various Chinese medicine compositions, traditional Chinese medicine ingredients are more complicated, the existing component beneficial to people, and have the component unfavorable to people's health, although can play some improves the health-care effect of some functions, general the problem of being unsuitable for long-term use.Health food is made of sea cucumber extract and earthworm albumen powder；Preparation method：First, the preparation of sea cucumber extract；2nd, the preparation of earthworm albumen powder；3rd, mix.The present invention is used for a kind of health food containing sea cucumber extract and earthworm albumen powder and preparation method thereof.
Description
Technical field
The present invention relates to a kind of health food and preparation method thereof.
Background technology
Hyperlipidemia refers to that blood lipid level is excessive, the disease that can directly cause some to seriously endanger health, such as artery
Atherosis, coronary heart disease etc..High fat is just frequently referred to since fat metabolism or operating exception are higher than blood plasma one or more lipid
Mass formed by blood stasis, lipid is insoluble or poorly soluble in water and must be combined with protein to be existed with lipoprotein form, and therefore, hyperlipidemia is often high fat
Proteinemia, shows as hypercholesterolemia, three vinegar mass formed by blood stasis of high glycerine etc., and hyperlipidemia by the diet prevention of health or can prolong
After occur, therefore, research and development are a kind of safe to use, and can effectively control the health food of hyperlipidemia to have important meaning
Justice.
At present, many health products all with the addition of various Chinese medicine compositions, and traditional Chinese medicine ingredients are more complicated, existing beneficial to people
Component, and have the component unfavorable to people's health, although can play some improves the health-care effect of some functions, generally not
It is adapted to long-term use, so, there is provided its security should be also fully ensured that while effective health products.
Sea cucumber, belongs to Holothuroidea (Holothuroidea), is the ocean echinoderm to 8000 meters by the sea that lives, away from the present
History more than 600,000,000 years existing, sea cucumber is using seabed algae and planktonic organism as food.Sea cucumber whole body covers with corn, and it is each to be widely distributed in the world
In ocean.China's Coastline of South China Sea species is more, and there are about more than 20 kind sea cucumbers can be edible, and the same ginseng of sea cucumber, bird's nest, shark's fin are neat
Name, is one of eight big treasure of the world.Sea cucumber is not only the food of preciousness, and rare medicinal material.According to《A Supplement to the Compendium of Materia Medica》In
Record：Sea cucumber, sweet-salty, kidney tonifying, benefiting quintessence take the photograph urine, and impotence, its warm-natured benefit, sufficient enemy's ginseng, therefore named sea cucumber are treated in establishing-Yang.Sea cucumber
With improve memory, delay sexual gland aging, prevent artery sclerosis and it is antitumor wait act on.
Modern medicine study also indicates that sea cucumber mainly contains sea cucumber polysaccharide, selenka (holothurin or sea cucumber cerebroysin), sea
Join collagen, sea cucumber polypeptide and a variety of amino acid, and the lipid components of multiple beneficial, as gangliosides, cerebroside and
Other unrighted acids.
In recent years, people are had found by the research increased to the various active ingredients of sea cucumber, these components of sea cucumber, which have, to be improved
Immunity of organisms, suppress the physiological functions such as tumour, anticoagulation.Have at the same time and adjust human immunity, antibacterial, antiviral, reduction blood
It is sugared, antifatigue and other effects.A variety of unrighted acid components are rich in the lipid of sea cucumber, there is reducing blood lipid, improve the circulatory system
The health-care effects such as function.
For earthworm protein extraction from earthworm, earthworm is one of important Chinese medicine in China.Earliest Chinese pharmacology monograph《Legendary god of farming's sheet
Grass warp》In just have earthworm in 67 kinds of animal drugs recording.There is heat-clearing arresting convulsion, dredging collateral, relieving asthma, diuresis.Earthworm except
Outer with better nutritivity value, it also has many healthcare functions, can strengthen the premunition of human body, and it is horizontal to improve people's health.
In recent ten years, the numerous studies in terms of chemistry, pharmacology have been carried out to earthworm both at home and abroad, have found earthworm not only containing abundant battalion
Form point, also containing a variety of pharmacological components, there is clostridiopetidase A, fibrinolysin, Lumbrokinase, nucleic acid, micro in active earthworm protein
The Multiple components such as element have many pharmacological actions, are related to each system of human body such as cardiovascular system, hematological system, are immunized
System, central nervous system, respiratory system, digestive system etc..Especially in terms of cardiovascular system and hematological system, earthworm energy
Preventing hypertension, miocardial infarction, arrhythmia cordis, apoplexy embolism etc. well.Such as Yang Xin is published in《Jianghan University's journal (from
Right science version)》Article on 1st P83-88 pages of the phase in 2017《Earthworm anticoagulant active Progress in Research of Substance》In over the ground dragon and its
The bioactivity of extract, has especially been used as careful description in the work of blood circulation system.Ground dragon protein is formal
It is included in national new resource food catalogue.
The content of the invention
The invention solves existing health products all with the addition of various Chinese medicine compositions, and traditional Chinese medicine ingredients are more complicated, existing right
The beneficial component of people, and have the component unfavorable to people's health, although can play some improves the health-care effect of some functions,
General the problem of being unsuitable for long-term use, and a kind of health food and its system containing sea cucumber extract Yu earthworm albumen powder are provided
Preparation Method.
A kind of health food containing sea cucumber extract and earthworm albumen powder is made of sea cucumber extract and earthworm albumen powder, institute
The mass ratio of the sea cucumber extract stated and earthworm albumen powder is 1:(0.4~2.5).
A kind of preparation method containing sea cucumber extract and the health food of earthworm albumen powder follows the steps below：
First, the preparation of sea cucumber extract：
1., unsalted dried sea cucumbers are soaked：
By unsalted dried sea cucumbers using the cold water wash that temperature is 2 DEG C~8 DEG C, temperature is then added into the sea cucumber after cleaning is
2 DEG C~8 DEG C of cold purified water simultaneously seals, by the sea cucumber after sealing be placed in temperature be soaked in 2 DEG C~8 DEG C of freezer 48h~
50h, the sea cucumber after being soaked；
The mass ratio for the cold purified water that sea cucumber after the cleaning is 2 DEG C~8 DEG C with temperature is 1:(5~6)；
2., homogenate：
By the sea cucumber after being soaked and after being soaked, remaining water breaks into homogenate with refiner, obtains homogenate；
3., freeze thawing extraction：
Homogenate is placed in the freezer that temperature is -25 DEG C~-20 DEG C and is freezed, until taken out after homogenate fully charge,
Homogenate after freezing is placed in thaw at RT, at a temperature of the homogenate of defrosting is positioned below 10 DEG C, supernatant is collected by centrifugation
Liquid and precipitation, by the degerming membrane filtration that supernatant aperture is 0.22 μm~0.25 μm, it is 2 DEG C~8 DEG C that filtrate, which is placed in temperature,
Saved backup in sterilized environment, obtain it is degerming after supernatant；
4., again freeze thawing extract：
The purified water that temperature is 2 DEG C~8 DEG C is added in the precipitation 3. obtained to step 1, then 3. freeze thawing carries by step 1
Take twice, will be degerming three times after supernatant merge, the supernatant after being merged；
The mass ratio for the purified water that 3. precipitation that the step one obtains is 2 DEG C~8 DEG C with temperature is 1:(5~6)；
5., be concentrated under reduced pressure：
Supernatant after merging is concentrated under reduced pressure, controls the temperature being concentrated under reduced pressure to be no more than 60 DEG C, pressure 70Pa
~80Pa, is concentrated into the 10%~12% of cumulative volume, the sea cucumber extract after being concentrated；
6., it is lyophilized：
By the degerming membrane filtration that the sea cucumber extract aperture after concentration is 0.22 μm~0.25 μm, then filtered fluid is put
Freezed in freeze dryer to water content and be less than 3%, obtain freeze-dried powder；
7., packaging：
Freeze-dried powder is sub-packed in aluminium plastic packaging bag simultaneously envelope, it is spare, obtain sea cucumber extract；
2nd, the preparation of earthworm albumen powder：
1., earthworm crush：
Earthworm is placed in the baking oven that temperature is 80 DEG C~100 DEG C and dries 2h~4h, is then crushed with pulverizer, crosses 80 mesh
~100 mesh sieves, obtain earthworm powder；
2., enzymolysis：
Earthworm powder is placed in enzymatic vessel, purified water is added, mixture is obtained, under agitation, by enzymatic vessel temperature
50 DEG C~55 DEG C are warming up to, the sodium hydroxide solution for being then 10%~12% with mass percent adjusts mixture ph and is
7.0~7.2, then neutral proteinase is added into enzymatic vessel, hydrolyze 4h~5h, the reaction solution after being digested；
The earthworm powder and the mass ratio of purified water are 1:(14~16)；The earthworm powder and the matter of neutral proteinase
Amount is than being 100:(3~5)；
3., inactivated proteases：
Enzymatic vessel temperature is warming up to 90 DEG C~95 DEG C by 50 DEG C~55 DEG C, and in the condition that temperature is 90 DEG C~95 DEG C
Under, 15min~20min is kept the temperature, cooling down to less than 30 DEG C, obtains the reaction solution of inactivated proteases again after insulation；
4., ultrafiltration：
The reaction solution of inactivated proteases is subjected to ultrafiltration for the ultrafilter of 100,000 dalton with molecular cut off, collects molecule
Amount is less than the filtrate of 100,000 dalton, the small molecule enzymolysis liquid clarified；
5., concentration：
The small molecule enzymolysis liquid of clarification is got to pump and is concentrated under reduced pressure in tank, controls the temperature being concentrated under reduced pressure to be no more than 60
DEG C, pressure is 65Pa~75Pa, is concentrated into 1/5th of cumulative volume, the small molecule enzymolysis liquid concentrated；
6., it is lyophilized：
Concentration small molecule enzymolysis liquid is placed in freeze dryer to be freezed to water content and is less than 3%, obtains lyophilized earthworm egg
White powder；
7., packaging：
Lyophilized earthworm albumen powder is fitted into food to be sealed with aluminium plastic bag, it is spare, obtain earthworm albumen powder；
3rd, mix：
Sea cucumber extract and earthworm albumen powder are uniformly mixed, the health care containing sea cucumber extract and earthworm albumen powder is obtained and eats
Product；
The mass ratio of the sea cucumber extract and earthworm albumen powder is 1:(0.4~2.5).
The beneficial effects of the invention are as follows：1st, the present invention is to be followed using sea cucumber and earthworm as main material production one kind with improvement
The health food of loop system function, health food of the invention have sufficient safety in utilization；Existing health products are solved all to add
Added various Chinese medicine compositions, traditional Chinese medicine ingredients are more complicated, the existing component beneficial to people, and have to people health it is unfavorable into
Point, although can play some improves the health-care effect of some functions, general the problem of being unsuitable for long-term use.
2nd, the dose of health products suggestion of the invention adult is daily 2.4 grams, it is of the invention containing sea cucumber extract and
The Halth-care composition of ground dragon protein has obvious effect for reducing blood fat, and after 30 days high lipid foods are raised, high fat control group is small
Three indexs of blood fat of mouse are exceeded, reach hyperlipidemia state, and in addition to feeding high lipid food raising, also watch and feed the present invention
Prepare containing sea cucumber extract with three indexs of experimental group blood fat of earthworm albumen powder health food compared with healthy control group,
There is no significant change (maximum fluctuation value is 3%).
3rd, sea cucumber extract, rather than the side using protease hydrolytic are extracted from sea cucumber using the method for multigelation
Method, farthest remains the original active skull cap components of sea cucumber, and can destroy many natural components by the method for enzymolysis
Activity；Meanwhile the ultrafiltration membrance filter through 100,000 Dalton molecular weights in extraction ground dragon protein, remove be not easy to absorb big point
Sub- material, only retains small-molecule substance of the molecular weight below 100,000 Dalton molecular weights, more conducively absorbs.
The present invention is used for a kind of health food containing sea cucumber extract and earthworm albumen powder and preparation method thereof.
Embodiment
Technical solution of the present invention is not limited to the embodiment of act set forth below, further include each embodiment it
Between any combination.
Embodiment one：A kind of health food containing sea cucumber extract Yu earthworm albumen powder described in present embodiment
It is made of sea cucumber extract and earthworm albumen powder, the mass ratio of the sea cucumber extract and earthworm albumen powder is 1:(0.4~
2.5)。
The beneficial effect of present embodiment is：1st, present embodiment is using sea cucumber and earthworm as a kind of tool of main material production
Having improves the health food of circulatory function, and the health food of present embodiment has sufficient safety in utilization；Solve existing
There are health products all to the addition of various Chinese medicine compositions, traditional Chinese medicine ingredients are more complicated, the existing component beneficial to people, and have and be good for people
The component of Kang Buli, although can play some improves the health-care effect of some functions, is generally unsuitable for long-term use
Problem.
2nd, the dose of the health products suggestion adult of present embodiment is daily 2.4 grams, and present embodiment contains sea cucumber
The Halth-care composition of extract and ground dragon protein has obvious effect for reducing blood fat, after 30 days high lipid foods are raised, high fat
Three indexs of blood fat of control group mice are exceeded, reach hyperlipidemia state, and in addition to feeding high lipid food raising, also watch
Feed three indexs of experimental group blood fat and health containing sea cucumber extract and earthworm albumen powder health food prepared by present embodiment
Control group compares, without significant change (maximum fluctuation value is 3%).
3rd, sea cucumber extract, rather than the side using protease hydrolytic are extracted from sea cucumber using the method for multigelation
Method, farthest remains the original active skull cap components of sea cucumber, and can destroy many natural components by the method for enzymolysis
Activity；Meanwhile the ultrafiltration membrance filter through 100,000 Dalton molecular weights in extraction ground dragon protein, remove be not easy to absorb big point
Sub- material, only retains small-molecule substance of the molecular weight below 100,000 Dalton molecular weights, more conducively absorbs.
Embodiment two：A kind of health food containing sea cucumber extract Yu earthworm albumen powder described in present embodiment
Preparation method follow the steps below：
First, the preparation of sea cucumber extract：
1., unsalted dried sea cucumbers are soaked：
By unsalted dried sea cucumbers using the cold water wash that temperature is 2 DEG C~8 DEG C, temperature is then added into the sea cucumber after cleaning is
2 DEG C~8 DEG C of cold purified water simultaneously seals, by the sea cucumber after sealing be placed in temperature be soaked in 2 DEG C~8 DEG C of freezer 48h~
50h, the sea cucumber after being soaked；
The mass ratio for the cold purified water that sea cucumber after the cleaning is 2 DEG C~8 DEG C with temperature is 1:(5~6)；
2., homogenate：
By the sea cucumber after being soaked and after being soaked, remaining water breaks into homogenate with refiner, obtains homogenate；
3., freeze thawing extraction：
Homogenate is placed in the freezer that temperature is -25 DEG C~-20 DEG C and is freezed, until taken out after homogenate fully charge,
Homogenate after freezing is placed in thaw at RT, at a temperature of the homogenate of defrosting is positioned below 10 DEG C, supernatant is collected by centrifugation
Liquid and precipitation, by the degerming membrane filtration that supernatant aperture is 0.22 μm~0.25 μm, it is 2 DEG C~8 DEG C that filtrate, which is placed in temperature,
Saved backup in sterilized environment, obtain it is degerming after supernatant；
4., again freeze thawing extract：
The purified water that temperature is 2 DEG C~8 DEG C is added in the precipitation 3. obtained to step 1, then 3. freeze thawing carries by step 1
Take twice, will be degerming three times after supernatant merge, the supernatant after being merged；
The mass ratio for the purified water that 3. precipitation that the step one obtains is 2 DEG C~8 DEG C with temperature is 1:(5~6)；
5., be concentrated under reduced pressure：
Supernatant after merging is concentrated under reduced pressure, controls the temperature being concentrated under reduced pressure to be no more than 60 DEG C, pressure 70Pa
~80Pa, is concentrated into the 10%~12% of cumulative volume, the sea cucumber extract after being concentrated；
6., it is lyophilized：
By the degerming membrane filtration that the sea cucumber extract aperture after concentration is 0.22 μm~0.25 μm, then filtered fluid is put
Freezed in freeze dryer to water content and be less than 3%, obtain freeze-dried powder；
7., packaging：
Freeze-dried powder is sub-packed in aluminium plastic packaging bag simultaneously envelope, it is spare, obtain sea cucumber extract；
2nd, the preparation of earthworm albumen powder：
1., earthworm crush：
Earthworm is placed in the baking oven that temperature is 80 DEG C~100 DEG C and dries 2h~4h, is then crushed with pulverizer, crosses 80 mesh
~100 mesh sieves, obtain earthworm powder；
2., enzymolysis：
Earthworm powder is placed in enzymatic vessel, purified water is added, mixture is obtained, under agitation, by enzymatic vessel temperature
50 DEG C~55 DEG C are warming up to, the sodium hydroxide solution for being then 10%~12% with mass percent adjusts mixture ph and is
7.0~7.2, then neutral proteinase is added into enzymatic vessel, hydrolyze 4h~5h, the reaction solution after being digested；
The earthworm powder and the mass ratio of purified water are 1:(14~16)；The earthworm powder and the matter of neutral proteinase
Amount is than being 100:(3~5)；
3., inactivated proteases：
Enzymatic vessel temperature is warming up to 90 DEG C~95 DEG C by 50 DEG C~55 DEG C, and in the condition that temperature is 90 DEG C~95 DEG C
Under, 15min~20min is kept the temperature, cooling down to less than 30 DEG C, obtains the reaction solution of inactivated proteases again after insulation；
4., ultrafiltration：
The reaction solution of inactivated proteases is subjected to ultrafiltration for the ultrafilter of 100,000 dalton with molecular cut off, collects molecule
Amount is less than the filtrate of 100,000 dalton, the small molecule enzymolysis liquid clarified；
5., concentration：
The small molecule enzymolysis liquid of clarification is got to pump and is concentrated under reduced pressure in tank, controls the temperature being concentrated under reduced pressure to be no more than 60
DEG C, pressure is 65Pa~75Pa, is concentrated into 1/5th of cumulative volume, the small molecule enzymolysis liquid concentrated；
6., it is lyophilized：
Concentration small molecule enzymolysis liquid is placed in freeze dryer to be freezed to water content and is less than 3%, obtains lyophilized earthworm egg
White powder；
7., packaging：
Lyophilized earthworm albumen powder is fitted into food to be sealed with aluminium plastic bag, it is spare, obtain earthworm albumen powder；
3rd, mix：
Sea cucumber extract and earthworm albumen powder are uniformly mixed, the health care containing sea cucumber extract and earthworm albumen powder is obtained and eats
Product；
The mass ratio of the sea cucumber extract and earthworm albumen powder is 1:(0.4~2.5).
Material such as sucrose, starch, dextrin, the microcrystalline cellulose as food production auxiliary material can be added in present embodiment
The auxiliary materials such as element, magnesium stearate, are made buccal tablet, oral tablet or oral liquor and other types of formulations according to general method, meet not
Difference with crowd requires.
The beneficial effect of present embodiment is：1st, present embodiment is using sea cucumber and earthworm as a kind of tool of main material production
Having improves the health food of circulatory function, and the health food of present embodiment has sufficient safety in utilization；Solve existing
There are health products all to the addition of various Chinese medicine compositions, traditional Chinese medicine ingredients are more complicated, the existing component beneficial to people, and have and be good for people
The component of Kang Buli, although can play some improves the health-care effect of some functions, is generally unsuitable for long-term use
Problem.
2nd, the dose of the health products suggestion adult of present embodiment is daily 2.4 grams, and present embodiment contains sea cucumber
The Halth-care composition of extract and ground dragon protein has obvious effect for reducing blood fat, after 30 days high lipid foods are raised, high fat
Three indexs of blood fat of control group mice are exceeded, reach hyperlipidemia state, and in addition to feeding high lipid food raising, also watch
Feed three indexs of experimental group blood fat and health containing sea cucumber extract and earthworm albumen powder health food prepared by present embodiment
Control group compares, without significant change (maximum fluctuation value is 3%).
3rd, sea cucumber extract, rather than the side using protease hydrolytic are extracted from sea cucumber using the method for multigelation
Method, farthest remains the original active skull cap components of sea cucumber, and can destroy many natural components by the method for enzymolysis
Activity；Meanwhile the ultrafiltration membrance filter through 100,000 Dalton molecular weights in extraction ground dragon protein, remove be not easy to absorb big point
Sub- material, only retains small-molecule substance of the molecular weight below 100,000 Dalton molecular weights, more conducively absorbs.
Embodiment three：Present embodiment is unlike embodiment two：Step 1 is 1. middle by after sealing
Sea cucumber to be placed in temperature be the 48h that is soaked in 2 DEG C~8 DEG C of freezer；Step 1 1. described in cleaning after sea cucumber and temperature be 2
DEG C~mass ratio of 8 DEG C of cold purified water is 1:5.It is other identical with embodiment two.
Embodiment four：Unlike one of present embodiment and embodiment two or three：Step 1 3. in
Homogenate is placed in the freezer that temperature is -25 DEG C and is freezed, until being taken out after homogenate fully charge, by the homogenate after freezing
Thaw at RT is placed in, at a temperature of the homogenate of defrosting is positioned below 10 DEG C, supernatant and precipitation is collected by centrifugation, by supernatant
With the degerming membrane filtration that aperture is 0.22 μm, filtrate is placed in the sterilized environment that temperature is 2 DEG C~8 DEG C and saves backup, removed
Supernatant after bacterium.It is other identical with embodiment two or three.
Embodiment five：Unlike one of present embodiment and embodiment two to four：Step 1 4. in
The mass ratio for the purified water that 3. precipitation that the step one obtains is 2 DEG C~8 DEG C with temperature is 1:5.Other and specific implementation
Mode two to four is identical.
Embodiment six：Unlike one of present embodiment and embodiment two to five：Step 1 5. in
Supernatant after merging is concentrated under reduced pressure, controls the temperature being concentrated under reduced pressure to be no more than 60 DEG C, pressure is 70Pa~80Pa, dense
It is reduced to the 10% of cumulative volume.It is other identical with embodiment two to five.
Embodiment seven：Unlike one of present embodiment and embodiment two to six：Step 1 6. in
Be 0.22 μm of degerming membrane filtration by the sea cucumber extract aperture after concentration, then filtered fluid is placed in freeze dryer freeze to
Water content is less than 3%, obtains freeze-dried powder.It is other identical with embodiment two to six.
Embodiment eight：Unlike one of present embodiment and embodiment two to seven：Step 2 1. in
Earthworm is placed in the baking oven that temperature is 80 DEG C and dries 2h, is then crushed with pulverizer, 80 mesh sieves is crossed, obtains earthworm powder.It is other
It is identical with embodiment two to seven.
Embodiment nine：Unlike one of present embodiment and embodiment two to eight：Step 2 2. in
Earthworm powder is placed in enzymatic vessel, purified water is added, obtains mixture, under agitation, enzymatic vessel temperature is warming up to 50
DEG C~55 DEG C, it is 7.0~7.2 that the sodium hydroxide solution for being then 10% with mass percent, which adjusts mixture ph, then to enzyme
Neutral proteinase is added in solution tank, hydrolyzes 4h, the reaction solution after being digested；Step 2 2. described in earthworm powder and purified water
Mass ratio be 1:15；Step 2 2. described in earthworm powder and neutral proteinase mass ratio be 100:3.Other and specific reality
It is identical to apply mode two to eight.
Embodiment ten：Unlike one of present embodiment and embodiment two to nine：Step 2 3. in
Enzymatic vessel temperature is warming up to 90 DEG C~95 DEG C by 50 DEG C~55 DEG C, and under conditions of temperature is 90 DEG C~95 DEG C, insulation
15min, cooling down to less than 30 DEG C, obtains the reaction solution of inactivated proteases again after insulation.Other and embodiment two
It is identical to nine.
Beneficial effects of the present invention are verified using following embodiments：
Embodiment one：
A kind of preparation method containing sea cucumber extract and the health food of earthworm albumen powder follows the steps below：
First, the preparation of sea cucumber extract：
1., unsalted dried sea cucumbers are soaked：
By unsalted dried sea cucumbers using the cold water wash that temperature is 5 DEG C, it is 5 DEG C that temperature is then added into the sea cucumber after cleaning
Cold purified water simultaneously seals, and the sea cucumber after sealing is placed in the freezer that temperature is 5 DEG C the 48h that is soaked, the sea cucumber after being soaked；
The mass ratio for the cold purified water that sea cucumber after the cleaning is 5 DEG C with temperature is 1:5；
2., homogenate：
By the sea cucumber after being soaked and after being soaked, remaining water breaks into homogenate with refiner, obtains homogenate；
3., freeze thawing extraction：
Homogenate is placed in the freezer that temperature is -25 DEG C and is freezed, until being taken out after homogenate fully charge, after freezing
Homogenate be placed in thaw at RT, at a temperature of the homogenate of defrosting is placed in 2 DEG C, supernatant and precipitation is collected by centrifugation, will be upper
Clear liquid aperture is 0.22 μm of degerming membrane filtration, and filtrate is placed in the sterilized environment that temperature is 5 DEG C and saves backup, obtains degerming
Supernatant afterwards；
4., again freeze thawing extract：
The purified water that temperature is 5 DEG C is added in the precipitation 3. obtained to step 1, then by step 1 3. freeze thawing extraction two
It is secondary, will be degerming three times after supernatant merge, the supernatant after being merged；
The mass ratio for the purified water that 3. precipitation that the step one obtains is 5 DEG C with temperature is 1:5；
5., be concentrated under reduced pressure：
Supernatant after merging is concentrated under reduced pressure, it is 50 DEG C, pressure 75Pa to control the temperature being concentrated under reduced pressure, concentration
To the 10% of cumulative volume, the sea cucumber extract after being concentrated；
6., it is lyophilized：
By the degerming membrane filtration that the sea cucumber extract aperture after concentration is 0.22 μm, filtered fluid is then placed in freeze dryer
In freeze to water content be 1%, obtain freeze-dried powder；
7., packaging：
Freeze-dried powder is sub-packed in aluminium plastic packaging bag simultaneously envelope, it is spare, obtain sea cucumber extract；
2nd, the preparation of earthworm albumen powder：
1., earthworm crush：
Earthworm is placed in the baking oven that temperature is 80 DEG C and dries 2h, is then crushed with pulverizer, 80 mesh sieves is crossed, obtains earthworm
Powder；
2., enzymolysis：
Earthworm powder is placed in enzymatic vessel, purified water is added, mixture is obtained, under agitation, by enzymatic vessel temperature
50 DEG C are warming up to, it is 7.0 that the sodium hydroxide solution for being then 10% with mass percent, which adjusts mixture ph, then to enzymatic vessel
Middle addition neutral proteinase, hydrolyzes 4h, the reaction solution after being digested；
The earthworm powder and the mass ratio of purified water are 1:15；The earthworm powder and the mass ratio of neutral proteinase are
100:3；
3., inactivated proteases：
Enzymatic vessel temperature is warming up to 90 DEG C by 50 DEG C, and under the conditions of at a temperature of 90 °C, 15min is kept the temperature, after insulation
Cooling down obtains the reaction solution of inactivated proteases to 20 DEG C again；
4., ultrafiltration：
The reaction solution of inactivated proteases is subjected to ultrafiltration for the ultrafilter of 100,000 dalton with molecular cut off, collects molecule
Amount is less than the filtrate of 100,000 dalton, the small molecule enzymolysis liquid clarified；
5., concentration：
The small molecule enzymolysis liquid of clarification is got to pump and is concentrated under reduced pressure in tank, it is 50 DEG C to control the temperature being concentrated under reduced pressure, pressure
Power is 70Pa, is concentrated into 1/5th of cumulative volume, the small molecule enzymolysis liquid concentrated；
6., it is lyophilized：
Concentration small molecule enzymolysis liquid is placed in freeze dryer to be freezed to water content be 1%, obtains lyophilized ground dragon protein
Powder；
7., packaging：
Lyophilized earthworm albumen powder is fitted into food to be sealed with aluminium plastic bag, it is spare, obtain earthworm albumen powder；
3rd, mix：
Sea cucumber extract and earthworm albumen powder are uniformly mixed, the health care containing sea cucumber extract and earthworm albumen powder is obtained and eats
Product；
The mass ratio of the sea cucumber extract and earthworm albumen powder is 7:3.
Long-term watch that health food manufactured in the present embodiment containing sea cucumber extract and earthworm albumen powder is carried out to mouse is fed
Experiment, it was demonstrated that its security, specific implementation method are as follows：
The small white mouse of 20 week old is chosen, by only weighing, weight is rejected and is less than 20 grams and the mouse more than 25 grams, choose weight
Healthy mice between 20-25 grams 12, is divided to two groups at random, and every group 6, one group is as a control group, and one group is used as experimental group,
It is 40mg/mL that the health food manufactured in the present embodiment containing sea cucumber extract and earthworm albumen powder is made into concentration with distilled water,
Under conditions of normal rearing conditions are constant, also give daily 1 milliliter of every mouse of experimental group and filled (equivalent to 40 milligrams)
The mode feeding (according to 60 kilograms of calculating of weight for each person, equivalent to people daily using 120 grams of amount) of stomach, continuous feeding 28
My god, the growing state of two groups of mouse is observed, healthy with mouse per weekly check mouse health status, no disease and weight must not
Standard is reduced to, while every group is respectively dissected 3 mouse, contrast mouse liver, spleen, answer indifference as qualification.Measure weekly small
In mouse weight record following table：
Table 1：Control group normally raises 28 days mouse weight record sheet (units：Gram)
Table 2：The experimental group gavage health food manufactured in the present embodiment containing sea cucumber extract and earthworm albumen powder is 28 days small
Mouse weight record sheet (unit：Gram)
| Cycle numbering | 1 | 2 | 3 | 4 | 5 | 6 | It is average |
| 0th week | 21.0 | 21.5 | 22.0 | 23.0 | 22.5 | 21.5 | 21.9 |
| 1st week | 21.0 | 22.0 | 22.0 | 23.5 | 22.5 | 22.0 | 22.2 |
| 2nd week | 21.5 | 22.0 | 22.5 | 23.5 | 23.0 | 22.0 | 22.5 |
| 3rd week | 21.5 | 22.5 | 22.5 | 23.5 | 23.0 | 22.5 | 22.6 |
| 4th week | 22.5 | 22.5 | 23.0 | 23.5 | 23.5 | 22.5 | 22.9 |
As a result：Mouse is watched feed 28 days after, do not occur dead mouse or disease event, observe health, meanwhile, according to upper
As can be seen that being tested through 28 days, control group and experimental mice weight do not decline table 1,2, and slightly rise, Weighing body
After weight, each 3 mouse of control group and experimental group are dissected respectively, contrasts the liver and spleen of two groups of mouse, is just
Normal kermesinus, no enlargement phenomenon, surface is smooth, 2 groups of indifferences.
This experiment is beneficial the result is that carrying out heavy dose of watching daily to mouse by experiment feeds 40 milligrams (equivalent to 60 public affairs
Jin weight adult takes 120 grams of amount daily, and the dose of the health products suggestion adult of the present invention is daily 2.4 grams) this reality
The Halth-care composition for applying example is still safe, is had no adverse effect to mouse health.
Reducing blood lipid zoopery：The blood fat reducing function of the present embodiment is proved by zoopery, specific test method is as follows：
The small white mouse of 20 week old is chosen, by only weighing, weight is rejected and is less than 20 grams and the mouse more than 25 grams, choose weight
Healthy mice between 20-25 grams 18, random point three groups, every group 6, one group is used as healthy control group, and two groups as high
Fat control group, three groups are used as experimental group, the 2.4 grams of calculating of usage amount suggested daily according to 60 kg body weights adult, every 20-25
Gram mouse should watch daily feed 0.9 milligram of health food manufactured in the present embodiment containing sea cucumber extract Yu earthworm albumen powder, institute
The health food manufactured in the present embodiment containing sea cucumber extract and earthworm albumen powder is made into concentration as 0.9mg/ with distilled water
ML, watches be fed for 1 milliliter of experimental mice (equivalent to 0.9 milligram) daily, and two groups small to high fat control group and experimental group at the same time
Mouse, which is watched, feeds identical high fat diet, is watched to healthy control group and feeds normal raising feed, and it is right after feeding 30 days, 30 days continuously to watch
Mouse carries out docking blood sampling, with the T-CHOL in three groups of mouse snow of medical Biochemical Analyzer chemical examination, the content of triglycerides, comments
The lipid-lowering effect of valency experimental group.
As a result：
Table 3：30 days lipid of mice testing results are tested in reducing blood lipid
Note：Experiment material source
1st, medical automatic clinical chemistry analyzer KQF-500 types, are produced by Yangzhou Kai Da Medical Devices Co., Ltd；
2nd, experimental animal：Small white mouse regular grade, Harbin veterinary institute Experimental Animal Center, while higher fatty acid feeding is provided
Material and normal culturing feed.
3rd, main agents triglyceride (TG) assay kit, T-CHOL (TC) assay kit, beta lipoprotein reagent
Box is provided by Shanghai Ji Ning industrial corporations.
It can be seen that from the experimental data of upper table after 30 days high lipid foods are raised, the blood fat of high fat control group mice
Three indexs are exceeded, reach hyperlipidemia state, so high fat control group is set up, meanwhile, the blood fat three of experimental group refers to
Mark is compared with healthy control group, without significant change (maximum fluctuation value is 3%).
So this experiment beneficial the result is that fully prove the present embodiment containing sea cucumber extract and ground dragon protein
Halth-care composition has obvious effect for reducing blood fat.
Claims (10)
1. a kind of health food containing sea cucumber extract Yu earthworm albumen powder, it is characterised in that one kind contains sea cucumber extract and earthworm
The health food of albumen powder is made of sea cucumber extract and earthworm albumen powder, the matter of the sea cucumber extract and earthworm albumen powder
Amount is than being 1:(0.4~2.5).
2. a kind of preparation method containing sea cucumber extract Yu the health food of earthworm albumen powder as claimed in claim 1, it is special
Sign is that a kind of preparation method containing sea cucumber extract and the health food of earthworm albumen powder follows the steps below：
First, the preparation of sea cucumber extract：
1., unsalted dried sea cucumbers are soaked：
By unsalted dried sea cucumbers using the cold water wash that temperature is 2 DEG C~8 DEG C, it is 2 DEG C that temperature is then added into the sea cucumber after cleaning
~8 DEG C of cold purified water simultaneously seals, and the sea cucumber after sealing is placed in the freezer that temperature is 2 DEG C~8 DEG C the 48h~50h that is soaked, is obtained
Sea cucumber to after being soaked；
The mass ratio for the cold purified water that sea cucumber after the cleaning is 2 DEG C~8 DEG C with temperature is 1:(5~6)；
2., homogenate：
By the sea cucumber after being soaked and after being soaked, remaining water breaks into homogenate with refiner, obtains homogenate；
3., freeze thawing extraction：
Homogenate is placed in the freezer that temperature is -25 DEG C~-20 DEG C and is freezed, until taken out after homogenate fully charge, will be cold
Homogenate after jelly is placed in thaw at RT, at a temperature of the homogenate of defrosting is positioned below 10 DEG C, be collected by centrifugation supernatant and
Precipitation, by the degerming membrane filtration that supernatant aperture is 0.22 μm~0.25 μm, filtrate is placed in the sterilizing that temperature is 2 DEG C~8 DEG C
Saved backup in environment, obtain it is degerming after supernatant；
4., again freeze thawing extract：
The purified water that temperature is 2 DEG C~8 DEG C is added in the precipitation 3. obtained to step 1, then by step 1 3. freeze thawing extraction two
It is secondary, will be degerming three times after supernatant merge, the supernatant after being merged；
The mass ratio for the purified water that 3. precipitation that the step one obtains is 2 DEG C~8 DEG C with temperature is 1:(5~6)；
5., be concentrated under reduced pressure：
Supernatant after merging is concentrated under reduced pressure, controls the temperature that is concentrated under reduced pressure to be no more than 60 DEG C, pressure for 70Pa~
80Pa, is concentrated into the 10%~12% of cumulative volume, the sea cucumber extract after being concentrated；
6., it is lyophilized：
By the degerming membrane filtration that the sea cucumber extract aperture after concentration is 0.22 μm~0.25 μm, filtered fluid is then placed in jelly
Freezed in dry machine to water content and be less than 3%, obtain freeze-dried powder；
7., packaging：
Freeze-dried powder is sub-packed in aluminium plastic packaging bag simultaneously envelope, it is spare, obtain sea cucumber extract；
2nd, the preparation of earthworm albumen powder：
1., earthworm crush：
Earthworm is placed in the baking oven that temperature is 80 DEG C~100 DEG C and dries 2h~4h, is then crushed with pulverizer, 80 mesh excessively~
100 mesh sieves, obtain earthworm powder；
2., enzymolysis：
Earthworm powder is placed in enzymatic vessel, purified water is added, obtains mixture, under agitation, enzymatic vessel temperature is heated up
To 50 DEG C~55 DEG C, the sodium hydroxide solution for being then 10%~12% with mass percent adjust mixture ph be 7.0~
7.2, then neutral proteinase is added into enzymatic vessel, hydrolyze 4h~5h, the reaction solution after being digested；
The earthworm powder and the mass ratio of purified water are 1:(14~16)；The earthworm powder and the mass ratio of neutral proteinase
For 100:(3~5)；
3., inactivated proteases：
Enzymatic vessel temperature is warming up to 90 DEG C~95 DEG C by 50 DEG C~55 DEG C, and under conditions of temperature is 90 DEG C~95 DEG C, is protected
Warm 15min~20min, cooling down to less than 30 DEG C, obtains the reaction solution of inactivated proteases again after insulation；
4., ultrafiltration：
The reaction solution of inactivated proteases is subjected to ultrafiltration for the ultrafilter of 100,000 dalton with molecular cut off, it is small to collect molecular weight
In the filtrate of 100,000 dalton, the small molecule enzymolysis liquid clarified；
5., concentration：
The small molecule enzymolysis liquid of clarification is got to pump and is concentrated under reduced pressure in tank, controls the temperature being concentrated under reduced pressure to be no more than 60 DEG C, pressure
Power is 65Pa~75Pa, is concentrated into 1/5th of cumulative volume, the small molecule enzymolysis liquid concentrated；
6., it is lyophilized：
Concentration small molecule enzymolysis liquid is placed in freeze dryer to be freezed to water content and is less than 3%, obtains lyophilized earthworm albumen powder；
7., packaging：
Lyophilized earthworm albumen powder is fitted into food to be sealed with aluminium plastic bag, it is spare, obtain earthworm albumen powder；
3rd, mix：
Sea cucumber extract and earthworm albumen powder are uniformly mixed, obtain the health food containing sea cucumber extract Yu earthworm albumen powder；
The mass ratio of the sea cucumber extract and earthworm albumen powder is 1:(0.4~2.5).
3. a kind of preparation method containing sea cucumber extract Yu the health food of earthworm albumen powder according to claim 2, its
The sea cucumber after sealing is placed in the freezer that temperature is 2 DEG C~8 DEG C the 48h that is soaked in being characterized in that step 1 1.；Step 1 1. in
The mass ratio for the cold purified water that sea cucumber after the cleaning is 2 DEG C~8 DEG C with temperature is 1:5.
4. a kind of preparation method containing sea cucumber extract Yu the health food of earthworm albumen powder according to claim 2, its
Homogenate is placed in the freezer that temperature is -25 DEG C in being characterized in that step 1 3. and is freezed, until being taken after homogenate fully charge
Go out, the homogenate after freezing is placed in thaw at RT, at a temperature of the homogenate of defrosting is positioned below 10 DEG C, is collected by centrifugation
Clear liquid and precipitation, by the degerming membrane filtration that supernatant aperture is 0.22 μm, filtrate is placed in the sterilizing ring that temperature is 2 DEG C~8 DEG C
Saved backup in border, obtain it is degerming after supernatant.
5. a kind of preparation method containing sea cucumber extract Yu the health food of earthworm albumen powder according to claim 2, its
The mass ratio of 3. precipitation that the step of described in being characterized in that step 1 4. one obtains and the purified water that temperature is 2 DEG C~8 DEG C is
1:5。
6. a kind of preparation method containing sea cucumber extract Yu the health food of earthworm albumen powder according to claim 2, its
The supernatant after merging is concentrated under reduced pressure in being characterized in that step 1 5., controls the temperature being concentrated under reduced pressure to be no more than 60 DEG C,
Pressure is 70Pa~80Pa, is concentrated into the 10% of cumulative volume.
7. a kind of preparation method containing sea cucumber extract Yu the health food of earthworm albumen powder according to claim 2, its
By the sea cucumber extract aperture after concentration it is 0.22 μm of degerming membrane filtration in being characterized in that step 1 6., then by filtered fluid
It is placed in freeze dryer to freeze to water content and is less than 3%, obtains freeze-dried powder.
8. a kind of preparation method containing sea cucumber extract Yu the health food of earthworm albumen powder according to claim 2, its
Earthworm is placed in the baking oven that temperature is 80 DEG C in being characterized in that step 2 1. and dries 2h, is then crushed with pulverizer, crosses 80 mesh
Sieve, obtains earthworm powder.
9. a kind of preparation method containing sea cucumber extract Yu the health food of earthworm albumen powder according to claim 2, its
Earthworm powder is placed in enzymatic vessel in being characterized in that step 2 2., purified water is added, obtains mixture, under agitation, will
Enzymatic vessel temperature is warming up to 50 DEG C~55 DEG C, then adjusts mixture ph with the sodium hydroxide solution that mass percent is 10%
For 7.0~7.2, then neutral proteinase is added into enzymatic vessel, hydrolyze 4h, the reaction solution after being digested；Step 2 2. middle institute
The earthworm powder and the mass ratio of purified water stated are 1:15；Step 2 2. described in earthworm powder and the mass ratio of neutral proteinase be
100:3。
10. a kind of preparation method containing sea cucumber extract Yu the health food of earthworm albumen powder according to claim 2, its
Enzymatic vessel temperature is warming up to 90 DEG C~95 DEG C by 50 DEG C~55 DEG C in being characterized in that step 2 3., and is 90 DEG C~95 in temperature
Under conditions of DEG C, 15min is kept the temperature, cooling down to less than 30 DEG C, obtains the reaction solution of inactivated proteases again after insulation.
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| CN115399482A (en) * | 2022-01-29 | 2022-11-29 | 中科国健(天津)生物工程有限公司 | Earthworm protein nutrition powder and nutrition paste for relieving gout and preparation method thereof |
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