CN107896991A - A kind of Jujun grasses artificial seed production method - Google Patents
A kind of Jujun grasses artificial seed production method Download PDFInfo
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- CN107896991A CN107896991A CN201711200147.5A CN201711200147A CN107896991A CN 107896991 A CN107896991 A CN 107896991A CN 201711200147 A CN201711200147 A CN 201711200147A CN 107896991 A CN107896991 A CN 107896991A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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Abstract
The invention discloses a kind of Jujun grasses artificial seed production method, comprise the following steps：S1, explant materials：The axillary bud of pennisetum sinese stem culm base is cut with pocket knife, as explant；S2, explant disinfection：Dried after axillary bud is sterilized, after cleaning spare；S3, seed bud finishing：Repaiied with pocket knife and cut upper end and irregularities, prepared for artificial seed；It is prepared by S4, artificial endosperm component：Sodium alginate, MS culture mediums, methyl α-naphthyl acetate, indolebutyric acid, sucrose and activated carbon mixing are taken, it is spare after sterilizing；It is prepared by S5, artificial seed bud capsule：Endosperm gel solution is injected in centrifuge tube, injects CaCl2Solution, forms Jujun grasses seed bud capsule after curing；S6, Artificial Seed Coats component and preparation：Sodium alginate, pulullan polysaccharide and sodium carboxymethylcellulose mixing are taken, it is spare after sterilizing；S7, Artificial Seed Coats bag are standby：Close the lid after artificial seed is put into capsule shells.The present invention is suitable for grass seeds Mechanization sowing and produces, and shortens the cultivation cycle of seed, reduces cost, improves production efficiency.
Description
Technical field
The present invention relates to Jujun grasses breeding technical field, more particularly to a kind of Jujun grasses artificial seed production method.
Background technology
Jujun grasses (Pennisetum giganteum z.x.lin) are the perennial upright bunch types of grass family Pennisetum
Plant, has stronger tillering ability, the general 3-4.5m of plant height.Originate in north African.The 80's of eighties of last century introduce China Fujian
Success is planted experimentally, through the continuous improvement culture experiment of more than 20 years, Jujun grasses were cultivated except being adapted to China's weather soil ring at present
The grass seeds in border.
The matrix such as the sawdust of tradition culture edible fungi substrate generally use are, it is necessary to substantial amounts of timber.And Jujun grasses can be replaced
It is used for the culture medium for cultivating edible and medical fungi for trees, so as to largely reduce the felling to trees, protects mountain forest, reduce water and soil stream
Lose.Jujun grasses can also improve soil, adjust P in soil H values.Jujun grasses contain abundant protein content, and wherein crude protein content is high
Up to 10%, crude fiber content 28.5%, crude fat content 3.8%, are good animal feeds, China pastoral area is to Jujun grasses
Demand it is very big.Since national policy is supported, plantation Jujun grasses can also obtain national special fund is subsidized and electricity price is subsidized etc.,
Therefore Jujun grasses industrial technology is promoted to be of great significance to improving farmers' income.
Planting huge fungous grass is simple, can both carry out generative propagation, also can vegetative propagation.Generative propagation percentage of seedgermination is extremely low,
Stem segment with axillary bud is commonly used in production vegetative propagation is carried out using short stem cuttage.By seasonal conditions and the shadow of long-distance transport condition
Ring, north seedling stem is transported to the north after generally being bred from south, and germination percentage is extremely low, and reproductive-cost is high；In addition, using cuttage
Breeding needs to consume substantial amounts of maternal plant, and the interception process of short stem used in cuttage needs to expend a large amount of manpower and materials,
Further increase the cost of Jujun grasses breeding.In contrast, artificial seed utilizes the feature of somatic embryo occur, it is embedded
In capsule, formed and have seed-bearing performance and directly in field sowing.Main advantage is to produce not being subject to seasonal restrictions；May
Quickly turn out new varieties come can also be added in gel wrappage natural seed may without advantageous component；Make manually
Seed has the ability of better nutrition supply and resist the disease, so that the possibility more to grow vigorously is obtained, easy to transport
It is defeated and be widely popularized.Therefore, the preparation of Jujun grasses artificial seed, is the popularization of Jujun grasses large-scale planting and breeding modes of reproduction
Using offer technical support.
The content of the invention
Based on technical problem existing for background technology, the present invention proposes a kind of Jujun grasses artificial seed production method.
A kind of Jujun grasses artificial seed production method proposed by the present invention, comprises the following steps：
S1, explant materials：The Jujun grasses stalk of maturation is taken, in soil, 22-33 DEG C of environment promotes at inter-species for oblique cuttage
Axillary bud sprouting, when axillary bud sprouting about 2-5cm high, is cut the axillary bud of pennisetum sinese stem culm base with pocket knife, as explant；
S2, explant disinfection：The axillary bud material of collection is put into 75% alcohol and sterilizes 30-40s, is then placed in 10%
9-12min is sterilized in liquor natrii hypochloritis；The axillary bud after disinfection is put into sterile tap water in sterile working and cleans 3-5
It is secondary, take out dry moisture afterwards, it is spare；
S3, seed bud finishing：Axillary bud after disinfection is repaiied with sterile pocket knife and cuts upper end and irregularities, leaves base portion
The cripetura section part of 1.4-1.6cm is prepared for artificial seed；
It is prepared by S4, artificial endosperm component：Artificial endosperm component is 2.5-3.5% sodium alginates, MS culture mediums, 1-3mg/L
Methyl α-naphthyl acetate, 0.2-0.5mg/L indolebutyric acids, 2-3% sucrose and 0.5-1g/L activated carbons, pH 5.8-6.2；110-130 DEG C of height
Warm autoclaving 20min；Cool down spare；
It is prepared by S5, artificial seed bud capsule：Endosperm gel solution 2-4ml is injected in 10-20ml centrifuge tubes；Inhaled with syringe
Take 3ml1.5%CaCl2Solution, syringe needle insertion centrifugation bottom of the tube simultaneously inject CaCl2Solution, after curing 10min, forms Jujun grasses
Seed bud capsule, with rinsed with sterile water 3 times；
S6, Artificial Seed Coats component and preparation：Artificial Seed Coats component is 5-7% sodium alginates, 0.8-1.2% pulullan polysaccharides
With 0.3-0.6% sodium carboxymethylcelluloses；110-130 DEG C of autoclave sterilization 20min postcooling is spare to room temperature；
S7, Artificial Seed Coats bag are standby：Artificial Jujun grasses seed bud capsule is immersed in Artificial Seed Coats solution, then after being pressed from both sides out with tweezers
It is put into 2%CaCl2Middle curing 20min, forms Jujun grasses Artificial Seed Coats, with rinsed with sterile water 3 times, dries surface moisture；Take conjunction
The capsule shells of suitable size, use ultraviolet radiation disinfection；After artificial seed is put into, capsule shells lid is finally covered, both obtains finished product
Jujun grasses artificial seed.
Preferably, will be stirred in the S2 in the explant transfer low-speed mixer by alcohol disinfecting with 30-60 revs/min
Mix so that explant disinfection is abundant.
Preferably, in the S4 artificial endosperm component for 3% sodium alginate, MS culture mediums, 2.5mg/L methyl α-naphthyl acetates,
0.5mg/L indolebutyric acids, 3% sucrose and 0.9g/L activated carbons.
Preferably, the amount of taking of endosperm gel solution and the specification of centrifuge tube are determined according to the actual size of seed bud in the S5
It is fixed, and be subject to seed bud and be put into endosperm gel solution and flooded completely.
Preferably, Artificial Seed Coats component obtained in the S6 is 6% sodium alginate, 1% pulullan polysaccharide and 0.5%
Sodium carboxymethylcellulose.
Preferably, then the S6 high temperatures autoclaving postcooling adds carbendazim extremely to room temperature into mixed solution
Concentration is 0.25-0.4%.
Preferably, the commercially available medical capsule shells of the capsule shells used in the S7, and can be determined according to artificial seed size
Do suitably sized.
Preferably, artificial seed is put into capsule shells in the S7, is remained with super absorbent resin filling inside capsule shells
Complementary space, for seed water suction after planting and water conservation.
Beneficial effects of the present invention are as follows：The seedling of Jujun grasses large-scale plantation is used for by the artificial seed of breeding
Demand, artificial seed prepared by this method, can make that the seedling strain heredity after breeding is good, does not undergo mutation, there is provided germination percentage, from
And protect the one's best quality of Juncao.Explant of the present invention using the axillary bud of rudiment between huge fungous grass as artificial seed, can be with
Make that the Jujun grasses seedling growth after breeding is more consistent, does not undergo mutation, further improve the quality of seedling.Use at the same time
Artificial seed prepared by Jujun grasses, can be adapted for mechanization production, shortens the tissue cultures cycle, reduce cost, improve
Production efficiency, while convenience in transport and storage, the present invention in a short time, meet business metaplasia by animal nutrition
The demand of production, technical support is provided for enterprise scale, industrialization culture high-quality Jujun grasses.
Brief description of the drawings
Fig. 1 is the production process figure of the present invention.
Embodiment
The present invention is made with reference to specific embodiment further to explain.
Embodiment 1
S1, explant materials：The Jujun grasses stalk of maturation is taken, in soil, 22 DEG C of environment promote armpit at inter-species for oblique cuttage
Bud is sprouted, and when axillary bud sprouting about 2cm high, is cut the axillary bud of pennisetum sinese stem culm base with pocket knife, as explant；
S2, explant disinfection：The axillary bud material of collection is put into 75% alcohol and sterilizes 30s, is then placed in 10% time
9min is sterilized in sodium chlorate solution；Axillary bud after disinfection is put into sterile tap water in the sterile working and cleans 3 times, afterwards
Moisture is dried in taking-up, spare；
S3, seed bud finishing：Axillary bud after disinfection is repaiied with sterile pocket knife and cuts upper end and irregularities, leaves base portion
The cripetura section part of 1.4cm is prepared for artificial seed；
It is prepared by S4, artificial endosperm component：Artificial endosperm component is 2.5% sodium alginate, MS culture mediums, 1.0mg/L naphthalene second
Acid, 0.2mg/L indolebutyric acids, 2% sucrose and 0.5g/L activated carbons, pH 5.8；110 DEG C of autoclave sterilization 20min；Cooling
It is spare；
It is prepared by S5, artificial seed bud capsule：Endosperm gel solution 2ml is injected in 10mL centrifuge tubes；3mL is drawn with syringe
1.5%CaCl2Solution, syringe needle insertion centrifugation bottom of the tube simultaneously inject CaCl2Solution, after curing 10min, forms Jujun grasses seed bud glue
Capsule, with rinsed with sterile water 3 times；
S6, Artificial Seed Coats component and preparation：Artificial Seed Coats component for 5% sodium alginate, 0.8% pulullan polysaccharide and
0.3% sodium carboxymethylcellulose；110 DEG C of autoclave sterilization 20min postcoolings are spare to room temperature；
S7, Artificial Seed Coats bag are standby：Artificial Jujun grasses seed bud capsule is immersed in Artificial Seed Coats solution, then after being pressed from both sides out with tweezers
It is put into 2%CaCl2Middle curing 20min, forms Jujun grasses Artificial Seed Coats, with rinsed with sterile water 3 times, dries surface moisture；Take conjunction
The capsule shells of suitable size, use ultraviolet radiation disinfection；After artificial seed is put into, capsule shells lid is finally covered, both obtains finished product
Jujun grasses artificial seed.
Embodiment 2
S1, explant materials：The Jujun grasses stalk of maturation is taken, in soil, 25 DEG C of environment promote armpit at inter-species for oblique cuttage
Bud is sprouted, and when axillary bud sprouting about 3cm high, is cut the axillary bud of pennisetum sinese stem culm base with pocket knife, as explant；
S2, explant disinfection：The axillary bud material of collection is put into 75% alcohol and sterilizes 35s, is then placed in 10% time
10min is sterilized in sodium chlorate solution；The axillary bud after disinfection is put into sterile tap water in sterile working and is cleaned 4 times, it
Take out afterwards and dry moisture, it is spare；
S3, seed bud finishing：Axillary bud after disinfection is repaiied with sterile pocket knife and cuts upper end and irregularities, leaves base portion
The cripetura section part of 1.5cm is prepared for artificial seed；
It is prepared by S4, artificial endosperm component：Artificial endosperm component is 3.0% sodium alginate, MS culture mediums, 1.5mg/L naphthalene second
Acid, 0.3mg/L indolebutyric acids, 23% sucrose and 0.7g/L activated carbons, pH 6.0；115 DEG C of autoclave sterilization 20min；Cooling
It is spare；
It is prepared by S5, artificial seed bud capsule：Endosperm gel solution 3mL is injected in 15ml centrifuge tubes；Drawn with syringe
3mL1.5%CaCl2Solution, syringe needle insertion centrifugation bottom of the tube simultaneously inject CaCl2Solution, after curing 10min, forms huge fungous grass
Bud capsule, with rinsed with sterile water 3 times；
S6, Artificial Seed Coats component and preparation：Artificial Seed Coats component for 6% sodium alginate, 1.0% pulullan polysaccharide and
0.4% sodium carboxymethylcellulose；115 DEG C of autoclave sterilization 20min postcoolings are spare to room temperature；
S7, Artificial Seed Coats bag are standby：Artificial Jujun grasses seed bud capsule is immersed in Artificial Seed Coats solution, then after being pressed from both sides out with tweezers
It is put into 2%CaCl2Middle curing 20min, forms Jujun grasses Artificial Seed Coats, with rinsed with sterile water 3 times, dries surface moisture；Take conjunction
The capsule shells of suitable size, use ultraviolet radiation disinfection；After artificial seed is put into, capsule shells lid is finally covered, both obtains finished product
Jujun grasses artificial seed.
Embodiment 3
S1, explant materials：The Jujun grasses stalk of maturation is taken, in soil, 28 DEG C of environment promote armpit at inter-species for oblique cuttage
Bud is sprouted, and when axillary bud sprouting about 3cm high, is cut the axillary bud of pennisetum sinese stem culm base with pocket knife, as explant；
S2, explant disinfection：The axillary bud material of collection is put into 75% alcohol and sterilizes 35s, is then placed in 10% time
10min is sterilized in sodium chlorate solution；The axillary bud after disinfection is put into sterile tap water in sterile working and is cleaned 4 times, it
Take out afterwards and dry moisture, it is spare；
S3, seed bud finishing：Axillary bud after disinfection is repaiied with sterile pocket knife and cuts upper end and irregularities, leaves base portion
The cripetura section part of 1.5cm is prepared for artificial seed；
It is prepared by S4, artificial endosperm component：Artificial endosperm component is 3.0% sodium alginate, MS culture mediums, 2.0mg/L naphthalene second
Acid, 0.4mg/L indolebutyric acids, 2.0% sucrose and 0.8g/L activated carbons, pH 6.0；120 DEG C of autoclave sterilization 20min；It is cold
It is spare；
It is prepared by S5, artificial seed bud capsule：Endosperm gel solution 3ml is injected in 15ml centrifuge tubes；Drawn with syringe
3ml1.5%CaCl2Solution, syringe needle insertion centrifugation bottom of the tube simultaneously inject CaCl2Solution, after curing 10min, forms huge fungous grass
Bud capsule, with rinsed with sterile water 3 times；
S6, Artificial Seed Coats component and preparation：Artificial Seed Coats component for 6% sodium alginate, 1.0% pulullan polysaccharide and
0.5% sodium carboxymethylcellulose；120 DEG C of autoclave sterilization 20min postcoolings are spare to room temperature；
S7, Artificial Seed Coats bag are standby：Artificial Jujun grasses seed bud capsule is immersed in Artificial Seed Coats solution, then after being pressed from both sides out with tweezers
It is put into 2%CaCl2Middle curing 20min, forms Jujun grasses Artificial Seed Coats, with rinsed with sterile water 3 times, dries surface moisture；Take conjunction
The capsule shells of suitable size, use ultraviolet radiation disinfection；After artificial seed is put into, capsule shells lid is finally covered, both obtains finished product
Jujun grasses artificial seed.
Embodiment 4
S1, explant materials：The Jujun grasses stalk of maturation is taken, in soil, 30 DEG C of environment promote armpit at inter-species for oblique cuttage
Bud is sprouted, and when axillary bud sprouting about 3cm high, is cut the axillary bud of pennisetum sinese stem culm base with pocket knife, as explant；
S2, explant disinfection：The axillary bud material of collection is put into 75% alcohol and sterilizes 35s, is then placed in 10% time
10min is sterilized in sodium chlorate solution；The axillary bud after disinfection is put into sterile tap water in sterile working and is cleaned 4 times, it
Take out afterwards and dry moisture, it is spare；
S3, seed bud finishing：Axillary bud after disinfection is repaiied with sterile pocket knife and cuts upper end and irregularities, leaves base portion
The cripetura section part of 1.5cm is prepared for artificial seed；
It is prepared by S4, artificial endosperm component：Artificial endosperm component is 3.0% sodium alginate, MS culture mediums, 2.5mg/L naphthalene second
Acid, 0.5mg/L indolebutyric acids, 3.0% sucrose and 0.9g/L activated carbons, pH 6.2；125 DEG C of autoclave sterilization 20min；It is cold
It is spare；
It is prepared by S5, artificial seed bud capsule：Endosperm gel solution 3ml is injected in 15ml centrifuge tubes；Drawn with syringe
3ml1.5%CaCl2Solution, syringe needle insertion centrifugation bottom of the tube simultaneously inject CaCl2Solution, after curing 10min, forms huge fungous grass
Bud capsule, with rinsed with sterile water 3 times；
S6, Artificial Seed Coats component and preparation：Artificial Seed Coats component for 6% sodium alginate, 1.0% pulullan polysaccharide and
0.5% sodium carboxymethylcellulose；125 DEG C of autoclave sterilization 20min postcoolings are spare to room temperature；
S7, Artificial Seed Coats bag are standby：Artificial Jujun grasses seed bud capsule is immersed in Artificial Seed Coats solution, then after being pressed from both sides out with tweezers
It is put into 2%CaCl2Middle curing 20min, forms Jujun grasses Artificial Seed Coats, with rinsed with sterile water 3 times, dries surface moisture；Take conjunction
The capsule shells of suitable size, use ultraviolet radiation disinfection；After artificial seed is put into, capsule shells lid is finally covered, both obtains finished product
Jujun grasses artificial seed.
Embodiment 5
S1, explant materials：The Jujun grasses stalk of maturation is taken, in soil, 33 DEG C of environment promote armpit at inter-species for oblique cuttage
Bud is sprouted, and when axillary bud sprouting about 5cm high, is cut the axillary bud of pennisetum sinese stem culm base with pocket knife, as explant；
S2, explant disinfection：The axillary bud material of collection is put into 75% alcohol and sterilizes 40s, is then placed in 10% time
12min is sterilized in sodium chlorate solution；The axillary bud after disinfection is put into sterile tap water in sterile working and is cleaned 5 times, it
Take out afterwards and dry moisture, it is spare；
S3, seed bud finishing：Axillary bud after disinfection is repaiied with sterile pocket knife and cuts upper end and irregularities, leaves base portion
The cripetura section part of 1.6cm is prepared for artificial seed；
It is prepared by S4, artificial endosperm component：Artificial endosperm component is 3.5% sodium alginate, MS culture mediums, 3mg/L naphthalene second
Acid, 0.5mg/L indolebutyric acids, 3.0% sucrose and 1.0g/L activated carbons, pH 6.2；130 DEG C of autoclave sterilization 20min；It is cold
It is spare；
It is prepared by S5, artificial seed bud capsule：Endosperm gel solution 4mL is injected in 20ml centrifuge tubes；Drawn with syringe
3ml1.5%CaCl2Solution, syringe needle insertion centrifugation bottom of the tube simultaneously inject CaCl2Solution, after curing 10min, forms huge fungous grass
Bud capsule, with rinsed with sterile water 3 times；
S6, Artificial Seed Coats component and preparation：Artificial Seed Coats component for 7% sodium alginate, 1.2% pulullan polysaccharide and
0.6% sodium carboxymethylcellulose；130 DEG C of autoclave sterilization 20min postcoolings are spare to room temperature；
S7, Artificial Seed Coats bag are standby：Artificial Jujun grasses seed bud capsule is immersed in Artificial Seed Coats solution, then after being pressed from both sides out with tweezers
It is put into 2%CaCl2Middle curing 20min, forms Jujun grasses Artificial Seed Coats, with rinsed with sterile water 3 times, dries surface moisture；Take conjunction
The capsule shells of suitable size, use ultraviolet radiation disinfection；After artificial seed is put into, capsule shells lid is finally covered, both obtains finished product
Jujun grasses artificial seed.
Upper table is embodiment 1-5 contrast experiment's tables.As seen from the above table, the percentage of seedgermination after being handled using embodiment 4 is most
Height, is not set forth in detail here.
The foregoing is only a preferred embodiment of the present invention, but protection scope of the present invention be not limited thereto,
Any one skilled in the art the invention discloses technical scope in, technique according to the invention scheme and its
Inventive concept is subject to equivalent substitution or change, should be covered by the protection scope of the present invention.
Claims (8)
1. a kind of Jujun grasses artificial seed production method, it is characterised in that comprise the following steps：
S1, explant materials：The Jujun grasses stalk of maturation is taken, in soil, 22-33 DEG C of environment promotes axillary bud at inter-species for oblique cuttage
Sprout, when axillary bud sprouting about 2-5cm high, cut the axillary bud of pennisetum sinese stem culm base with pocket knife, as explant；
S2, explant disinfection：The axillary bud material of collection is put into 75% alcohol and sterilizes 30-40s, is then placed in 10% chlorine
9-12min is sterilized in acid sodium solution；The axillary bud after disinfection is put into sterile tap water in sterile working and is cleaned 3-5 times,
Take out afterwards and dry moisture, it is spare；
S3, seed bud finishing：Axillary bud after disinfection is repaiied with sterile pocket knife and cuts upper end and irregularities, leaves base portion 1.4-
The cripetura section part of 1.6cm is prepared for artificial seed；
It is prepared by S4, artificial endosperm component：Artificial endosperm component is 2.5-3.5% sodium alginates, MS culture mediums, 1-3mg/L naphthalene second
Acid, 0.2-0.5mg/L indolebutyric acids, 2-3% sucrose and 0.5-1g/L activated carbons, pH 5.8-6.2；110-130 DEG C of high temperature height
Pressure sterilizing 20min；Cool down spare；
It is prepared by S5, artificial seed bud capsule：Endosperm gel solution 2-4mL is injected in 10-20mL centrifuge tubes；Drawn with syringe
3mL 1.5%CaCl2Solution, syringe needle insertion centrifugation bottom of the tube simultaneously inject CaCl2Solution, after curing 10min, forms huge fungous grass
Bud capsule, with rinsed with sterile water 3 times；
S6, Artificial Seed Coats component and preparation：Artificial Seed Coats component for 5-7% sodium alginates, 0.8-1.2% pulullan polysaccharides and
0.3-0.6% sodium carboxymethylcelluloses；110-130 DEG C of autoclave sterilization 20min postcooling is spare to room temperature；
S7, Artificial Seed Coats bag are standby：Artificial Jujun grasses seed bud capsule is immersed in Artificial Seed Coats solution, then is put into after being pressed from both sides out with tweezers
2%CaCl2Middle curing 20min, forms Jujun grasses Artificial Seed Coats, with rinsed with sterile water 3 times, dries surface moisture；Take suitable big
Small capsule shells, use ultraviolet radiation disinfection；After artificial seed is put into, capsule shells lid is finally covered, both obtains the huge bacterium of finished product
Careless artificial seed.
2. a kind of Jujun grasses artificial seed production method according to claim 1, it is characterised in that will pass through in the S2
With 30-60 revs/min of stirring in the explant transfer low-speed mixer of alcohol disinfecting so that explant disinfection is abundant.
A kind of 3. Jujun grasses artificial seed production method according to claim 1, it is characterised in that artificial embryo in the S4
Milk composition is 3% sodium alginate, MS culture mediums, 2.5mg/L methyl α-naphthyl acetates, 0.5mg/L indolebutyric acids, 3% sucrose and 0.9g/L are lived
Property charcoal.
4. a kind of Jujun grasses artificial seed production method according to claim 1, it is characterised in that endosperm coagulates in the S5
The amount of taking of sol solution and the specification of centrifuge tube are determined according to the actual size of seed bud, and are put into seed bud in endosperm gel solution
Subject to being flooded completely.
5. a kind of Jujun grasses artificial seed production method according to claim 1, it is characterised in that obtained in the S6
Artificial Seed Coats component be 6% sodium alginate, 1% pulullan polysaccharide and 0.5% sodium carboxymethylcellulose.
6. a kind of Jujun grasses artificial seed production method according to claim 1, it is characterised in that the S6 high temperatures are high
For pressure sterilizing postcooling to room temperature, it is 0.25-0.4% that carbendazim to concentration is then added into mixed solution.
7. a kind of Jujun grasses artificial seed production method according to claim 1, it is characterised in that used in the S7
Capsule shells with commercially available medical capsule shells, and can be customized suitably sized according to artificial seed size.
8. a kind of Jujun grasses artificial seed production method according to claim 1, it is characterised in that to capsule in the S7
Artificial seed is put into shell, inside capsule shells remaining space is filled with super absorbent resin, for the water suction of seed after planting and
Water conservation.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110235782A (en) * | 2019-06-17 | 2019-09-17 | 西安同人五凤农业有限公司 | A kind of production method of elaeagnus mollis artificial seed |
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| CN102428870A (en) * | 2011-09-22 | 2012-05-02 | 澄思源生物科技（上海）有限公司 | Method for preparing artificial seeds of dendrobium officinale |
| US20120329158A1 (en) * | 2010-01-13 | 2012-12-27 | Prakash Lakshmanan | Methods of Plant Regeneration and Apparatus Therefor |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| US20120329158A1 (en) * | 2010-01-13 | 2012-12-27 | Prakash Lakshmanan | Methods of Plant Regeneration and Apparatus Therefor |
| CN102428870A (en) * | 2011-09-22 | 2012-05-02 | 澄思源生物科技（上海）有限公司 | Method for preparing artificial seeds of dendrobium officinale |
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| CN110235782A (en) * | 2019-06-17 | 2019-09-17 | 西安同人五凤农业有限公司 | A kind of production method of elaeagnus mollis artificial seed |
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Effective date of registration: 20200819 Address after: 571799 Eighth Team of Nanchen Farm, Danzhou City, Hainan Province Patentee after: DANZHOU MUCHUN GREEN ECOLOGICAL AGRICULTURE DEVELOPMENT Co.,Ltd. Address before: 570000 No. 4, Longhua, Haikou District, Hainan, Xueyuan Road Co-patentee before: DANZHOU MUCHUN GREEN ECOLOGICAL AGRICULTURE DEVELOPMENT Co.,Ltd. Patentee before: INSTITUTE OF TROPICAL BIOSCIENCE AND BIOTECHNOLOGY, CHINESE ACADEMY OF TROPICAL AGRICULTURAL SCIENCES |