CN107881087B - Detection device for food microorganisms - Google Patents
Detection device for food microorganisms Download PDFInfo
- Publication number
- CN107881087B CN107881087B CN201711437357.6A CN201711437357A CN107881087B CN 107881087 B CN107881087 B CN 107881087B CN 201711437357 A CN201711437357 A CN 201711437357A CN 107881087 B CN107881087 B CN 107881087B
- Authority
- CN
- China
- Prior art keywords
- crystal oscillator
- arc
- metal contact
- culture medium
- base
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 244000005700 microbiome Species 0.000 title claims abstract description 43
- 238000001514 detection method Methods 0.000 title claims abstract description 22
- 235000013305 food Nutrition 0.000 title claims abstract description 15
- 239000013078 crystal Substances 0.000 claims abstract description 102
- 239000001963 growth medium Substances 0.000 claims abstract description 31
- 239000002184 metal Substances 0.000 claims description 26
- 239000007788 liquid Substances 0.000 claims description 15
- 238000003825 pressing Methods 0.000 claims description 13
- 239000012488 sample solution Substances 0.000 claims description 11
- 230000005284 excitation Effects 0.000 claims description 9
- 238000005286 illumination Methods 0.000 claims description 7
- 229920001296 polysiloxane Polymers 0.000 claims description 6
- 238000005452 bending Methods 0.000 claims description 3
- 238000007789 sealing Methods 0.000 claims description 3
- 238000004904 shortening Methods 0.000 abstract description 2
- 239000002537 cosmetic Substances 0.000 description 35
- 238000011109 contamination Methods 0.000 description 14
- 238000004519 manufacturing process Methods 0.000 description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 11
- 239000002994 raw material Substances 0.000 description 9
- 230000000813 microbial effect Effects 0.000 description 8
- 238000004088 simulation Methods 0.000 description 6
- 238000000034 method Methods 0.000 description 5
- 238000004659 sterilization and disinfection Methods 0.000 description 5
- 230000003749 cleanliness Effects 0.000 description 4
- 239000007789 gas Substances 0.000 description 4
- 238000004806 packaging method and process Methods 0.000 description 4
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical group O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 3
- 235000015097 nutrients Nutrition 0.000 description 3
- 239000000741 silica gel Substances 0.000 description 3
- 229910002027 silica gel Inorganic materials 0.000 description 3
- 230000001954 sterilising effect Effects 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 230000004308 accommodation Effects 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 239000011248 coating agent Substances 0.000 description 2
- 238000000576 coating method Methods 0.000 description 2
- 239000008367 deionised water Substances 0.000 description 2
- 229910021641 deionized water Inorganic materials 0.000 description 2
- 239000000428 dust Substances 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000003287 optical effect Effects 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 206010052748 Bacterial allergy Diseases 0.000 description 1
- 208000035143 Bacterial infection Diseases 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000007815 allergy Effects 0.000 description 1
- 208000022362 bacterial infectious disease Diseases 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 239000000084 colloidal system Substances 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000004945 emulsification Methods 0.000 description 1
- 239000003995 emulsifying agent Substances 0.000 description 1
- 239000000839 emulsion Substances 0.000 description 1
- 230000029142 excretion Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 239000004519 grease Substances 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 239000005022 packaging material Substances 0.000 description 1
- 238000012858 packaging process Methods 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 229920005862 polyol Polymers 0.000 description 1
- 150000003077 polyols Chemical class 0.000 description 1
- 239000003755 preservative agent Substances 0.000 description 1
- 230000002335 preservative effect Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 210000004761 scalp Anatomy 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000008399 tap water Substances 0.000 description 1
- 235000020679 tap water Nutrition 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- 231100000765 toxin Toxicity 0.000 description 1
- 108700012359 toxins Proteins 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M31/00—Means for providing, directing, scattering or concentrating light
- C12M31/08—Means for providing, directing, scattering or concentrating light by conducting or reflecting elements located inside the reactor or in its structure
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M31/00—Means for providing, directing, scattering or concentrating light
- C12M31/10—Means for providing, directing, scattering or concentrating light by light emitting elements located inside the reactor, e.g. LED or OLED
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/06—Means for regulation, monitoring, measurement or control, e.g. flow regulation of illumination
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Sustainable Development (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Analytical Chemistry (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a detection device for food microorganisms, which comprises: the shell comprises a base and an upper cover, wherein the base and the upper cover form an accommodating space together, and the upper cover can be hermetically covered on the base; the supporting table is arranged in the accommodating space, and an arc-shaped reflecting structure is arranged on the upper surface of the supporting table; the crystal oscillator device comprises a crystal oscillator bracket and a crystal oscillator arranged on the crystal oscillator bracket, the crystal oscillator device is arranged on the supporting table, a hollowed-out structure is arranged between the crystal oscillator bracket and the crystal oscillator, the lower surface of the crystal oscillator is positioned at the focus of the arc-shaped reflecting structure, and the lower surface of the crystal oscillator is provided with a culture medium; the simulated sunlight device is arranged above the crystal oscillator device, and light emitted by the simulated sunlight device irradiates the arc-shaped reflecting structure through the hollow structure and is focused on a culture medium on the lower surface of the crystal oscillator through the arc-shaped reflecting structure. The invention provides a microorganism detection device capable of shortening the culture time and improving the detection accuracy.
Description
Technical Field
The invention relates to the technical field of microorganism detection, in particular to a detection device for food microorganisms, which can shorten the culture time and improve the detection accuracy.
Background
With the development of economy, the living standard of people is continuously improved, pursuit of material culture life is higher and higher, cosmetics are indispensable daily living goods for vast loving people, and quality accidents of cosmetics frequently occur.
In fact, microorganisms in cosmetics are widely existed in nature, because the raw materials and additives of cosmetics contain a large amount of nutrients such as grease, colloid, protein, polyol and other nutrients and have a large amount of water, the nutrients such as carbon sources, nitrogen sources and water which are necessary for the growth and reproduction of the microorganisms, the microorganisms grow and reproduce in a large amount in the cosmetics under the conditions of proper temperature, humidity and the like, when the microorganisms reproduce to a considerable amount, on the one hand, the microorganisms absorb and decompose the effective components in the cosmetics, and on the other hand, new substances are generated due to excretion, so that the components of the cosmetics are destroyed, mildewed and spoiled, the cosmetics are discolored (red, black, green and other mildew spots are generated), unpleasant odor is generated, and the microorganisms also generate toxins, so that consumers can suffer from bacterial infection, allergy and other serious hazards. The microbial contamination of cosmetics can be divided into two cases, and the microbial contamination received during the production process of cosmetics is generally referred to as primary contamination; and contamination experienced by the consumer during use is referred to as secondary contamination. 1. The first-stage microbial contamination of cosmetics refers to the contamination in the production process of cosmetics, including the contamination of equipment, raw materials, production and packaging. Production equipment such as various transfer pumps, grinders, mixers, emulgators, filling machines and the like are places where microorganisms accumulate. Therefore, it is necessary to perform disinfection, sterilization, etc. Cosmetic raw materials are a source of contamination for cosmetic microorganisms. May have been contaminated prior to fabrication such as heating, mixing. Therefore, the raw materials, mainly animal and plant materials, are prevented from being polluted before production, especially the raw materials such as protein and starch. Before production, raw materials need to be subjected to spot check and microbial cultivation counting. The microorganism in the raw material is directly related to the product, the microorganism number of the raw material or the product should be controlled to be lower than 100 microorganisms/gram, and pathogenic bacteria cannot exist. In addition to disinfection and sterilization of raw materials, microbial contamination of water should be noted. Deionized water used in the production cannot be stored. In summer, the microorganism in deionized water can reach 1 million/g, and bacteria are polluted in tap water, so that the water is treated, and the water is usually disinfected by adopting methods such as heating, ultraviolet irradiation and the like. In the production and manufacture of cosmetics, such as emulsion, a heat sterilization method is generally adopted, water is heated to 90 ℃ for 20 minutes to sterilize, and then emulsification is carried out with oil phase of similar temperature. The final process package of cosmetic production is also a link which is easy to cause microbial contamination. Air cleaning is required in packaging rooms, and the average number of dust particles of >0.5 μm per liter of air represents the grade. In recent years, the classification of air cleanliness in China has started to adopt the American standard, and the expression method is as follows: the average number of dust particles with the air cleanliness grade of more than 0.5 μm is 100 grade (I) <3.5 grade (II) <35 grade (III) <350 grade (IV) < 100000 grade (IV) <3500 grade (IV), and the air cleanliness is between grade 10000 grade (III) and 100000 grade (IV). Grade III is a sterile workshop, and grade (IV) is a semi-sterile workshop. The packaging room can be checked for a clean sterile room by a bacterial culture method. In the cosmetic packaging process, the human body and clothes of the operator also cause serious contamination of microorganisms, for example, the microorganisms on the scalp of the human body can reach 140 ten thousand/cm < 2 >, so that the operator needs to be required to have high-standard personal hygiene, and the applied clothes, caps, shoes and the like are also sterilized. In addition, packaging containers, bottles, tubes, etc. for cosmetics are also contaminated with microorganisms, and glass instruments are generally more contaminated than plastic instruments, so that the packaging materials must be sterilized. 2. Microbial secondary contamination of cosmetics this microbial contamination means that when a consumer uses a cosmetic, such as when applying the cosmetic with a finger, the cosmetic is contaminated by the microorganisms on the finger, and the lid of the cosmetic product is often opened and also contaminated by the microorganisms, so that the microorganisms also propagate rapidly in the cosmetic, causing the cosmetic to go moldy, spoilage, etc. Therefore, in the production of cosmetics, it is necessary to add an effective preservative to the cosmetics. Inhibit the proliferation of microorganisms.
The method for detecting the microorganisms in the cosmetics is an essential important component for quality management of the cosmetics, and can effectively ensure the use safety of users. The detection of the microorganisms in the cosmetics is one of important indexes for measuring the quality of the cosmetics, and is also one of scientific bases for judging whether the detected cosmetics are safely used or not. In addition, the cleanliness of the cosmetic processing environment can be judged through the detection of the microorganisms of the cosmetics, so that the degree of bacterial pollution of the cosmetics can be accurately evaluated, and a scientific basis is provided for various health management works.
Disclosure of Invention
The invention aims to provide a microorganism culture device capable of shortening culture time and improving detection accuracy.
In order to achieve the above purpose, the technical scheme provided by the invention is as follows: there is provided a detection device for food microorganisms, comprising:
the shell comprises a base and an upper cover, wherein the base and the upper cover jointly form an accommodating space, and the upper cover can be hermetically covered on the base;
the supporting table is arranged in the accommodating space, and an arc-shaped reflecting structure is arranged on the upper surface of the supporting table;
the crystal oscillator device comprises a crystal oscillator bracket and a crystal oscillator arranged on the crystal oscillator bracket, the crystal oscillator device is arranged on the supporting table, a hollowed-out structure is further arranged between the crystal oscillator bracket and the crystal oscillator, a filter lens is further inlaid on the hollowed-out structure, the lower surface of the crystal oscillator is positioned at the focus of the arc-shaped reflecting structure, and a culture medium is arranged on the lower surface of the crystal oscillator;
the liquid supply system comprises a sample solution supply pipe, a culture medium solution supply pipe, a discharge pipe, a four-way electromagnetic valve and a liquid supply main pipe, wherein the liquid supply main pipe penetrates through the bottom of the base and is connected to the center of the bottom of the arc-shaped reflecting structure, and the liquid supply main pipe is respectively communicated with the sample solution supply pipe, the culture medium solution supply pipe and the discharge pipe through the four-way electromagnetic valve;
the simulated sunlight device is arranged above the crystal oscillator device, and light emitted by the simulated sunlight device irradiates the arc-shaped reflecting structure through the hollow structure and is focused on the culture medium on the lower surface of the crystal oscillator through the arc-shaped reflecting structure.
The upper surface and the lower surface of the crystal oscillator are respectively provided with a foil-shaped excitation electrode, the foil-shaped excitation electrodes positioned on the upper surface of the crystal oscillator are led out along the upper surface of the crystal oscillator and are connected to the left side of the upper surface of the crystal oscillator support, the foil-shaped excitation electrodes positioned on the lower surface of the crystal oscillator are led out along the lower surface of the crystal oscillator and are connected to the right side of the upper surface of the crystal oscillator support, and the left side and the right side of the upper surface of the crystal oscillator support are respectively provided with a first metal contact used for being connected with an external circuit.
The crystal oscillator device comprises a supporting table, at least two crystal oscillator devices and at least two crystal oscillator device compressing components, wherein the crystal oscillator device compressing components comprise fixing parts and compressing parts, the fixing parts are fixed above the supporting table, and the compressing parts are formed by downwards bending the fixing parts and compress and fix the crystal oscillator devices.
The compressing part comprises a conductive wire, the conductive wire comprises a wire arranged in the compressing part and a second metal contact arranged on the end face of the compressing part, and the second metal contact is opposite to the first metal contact.
The solar simulation device further comprises an optical filter, wherein the optical filter is arranged below the solar simulation device.
The culture medium culture device further comprises a motor driving system, an output shaft of the motor driving system passes through the upper cover in a sealing way and is connected with the sunlight simulating device, and the motor driving system drives the sunlight simulating device to rotate, so that the culture medium obtains uniform reflection illumination.
The base and the upper cover are of circular structures, and further comprise an air inlet channel and an air outlet channel, wherein the air inlet channel and the air outlet channel are respectively arranged in the tangential direction of the lower part and the upper part of the base, the air inlet channel and the air outlet channel are arranged in opposite directions, and air entering from the air inlet channel forms spiral upward air flow in the accommodating space, and is finally discharged from the air outlet channel.
The crystal oscillator support comprises an inner ring structure, connecting columns and an outer ring structure, wherein the connecting columns are uniformly distributed in the inner ring structure and the outer ring structure, a hollow structure is formed between every two connecting columns, the inner side wall of the inner ring structure is inwards recessed to form a circumferential groove, and the crystal oscillator is embedded in the circumferential groove.
The connecting column is of a transparent structure.
The compressing part is a silica gel sucker structure, the second metal contact is located at the center of the silica gel sucker structure, and when the fixing part is fixed above the supporting table, the silica gel sucker structure compresses the periphery of the first metal contact, and the second metal contact is in good contact with the first metal contact.
Compared with the prior art, the detection device for the food microorganisms comprises the crystal oscillator device and the sunlight simulating device, a hollowed-out structure is arranged between the crystal oscillator support and the crystal oscillator, the lower surface of the crystal oscillator is arranged at the focus of the arc-shaped reflecting structure, and the lower surface of the crystal oscillator is provided with the culture medium, so that the microorganism detection under the irradiation conditions of aerobic, anaerobic and sunlight simulating can be performed by only immersing or coating the culture medium with the sample solution and then placing the culture medium in the detection device.
The invention will become more apparent from the following description taken in conjunction with the accompanying drawings which illustrate embodiments of the invention.
Drawings
FIG. 1 is a schematic view of an embodiment of the detection device for food microorganisms of the present invention.
Fig. 2 is another angular view of the detection device for food microorganisms shown in fig. 1.
Fig. 3 is a schematic view of a crystal oscillator device of the detecting device for food microorganisms shown in fig. 1.
FIG. 4 is a cross-sectional view of the inner annular structure of the crystal oscillator support of the detection device for food microorganisms as shown.
Detailed Description
Embodiments of the present invention will now be described with reference to the drawings, wherein like reference numerals represent like elements throughout. As described above, as shown in fig. 1, 2 and 3, the detection device 100 for food microorganisms according to the embodiment of the present invention includes:
the shell 1, the said shell 1 includes the base 11 and upper cover 12, the said base 11 and upper cover 12 jointly form a accommodation space 13, the said upper cover 12 can cover and locate on the said base 11 sealingly, can make the said accommodation space 13 form a airtight space;
the supporting table 2 is arranged in the accommodating space 13, and an arc-shaped reflecting structure 21 is arranged on the upper surface of the supporting table 2;
the crystal oscillator device 3, the crystal oscillator device 3 includes a crystal oscillator bracket 31 and a crystal oscillator 32 installed on the crystal oscillator bracket 31, the crystal oscillator device 3 is installed on the supporting table 2, a hollowed-out structure 33 is further arranged between the crystal oscillator bracket 31 and the crystal oscillator 32, a filter (not shown in the figure) is further inlaid on the hollowed-out structure 33, the lower surface of the crystal oscillator 32 is located at the focus of the arc-shaped reflecting structure 21, and a culture medium is arranged on the lower surface of the crystal oscillator 32;
a liquid supply system 8, wherein the liquid supply system 8 comprises a sample solution supply pipe 81, a culture medium solution supply pipe 82, a discharge pipe 83, a four-way electromagnetic valve 84 and a liquid supply main pipe 85, the liquid supply main pipe 85 passes through the bottom of the base 11 and is connected to the center of the bottom of the arc-shaped reflecting structure 21, and the liquid supply main pipe 81 is respectively communicated with the sample solution supply pipe 81, the culture medium solution supply pipe 82 and the discharge pipe 83 through the four-way electromagnetic valve 84; the sample solution or the culture medium solution can be controlled to enter the main liquid supply pipe 85 through the four-way electromagnetic valve 84 by the sample solution supply pipe 81 or the culture medium solution supply pipe 82, and then is injected into the arc-shaped reflecting structure 21 by the main liquid supply pipe 85, and the culture medium must be fully contacted with the sample solution or the culture medium solution.
The sunlight simulating device 4 is arranged above the crystal oscillator device 3, and light emitted by the sunlight simulating device 4 irradiates the arc-shaped reflecting structure 21 through the hollow structure 33 and is focused on the culture medium on the lower surface of the crystal oscillator 32 through the arc-shaped reflecting structure 21. The sunlight simulating device 4 has at least two functions, namely, the temperature in the accommodating space 13 is controlled by illumination, namely, the luminous power of the sunlight simulating device 4 is controlled, so that the effect of controlling the temperature of the accommodating space 13 is achieved, and illumination can be prevented from being reflected to the culture medium according to actual conditions; secondly, under the condition that the microorganisms cultured by the culture medium are cultured under illumination conditions, the test requirements are met by controlling the luminous power and the color light types of the sunlight simulating device 4.
It should be noted that, if the sunlight simulating device 4 is to be started, the arc reflecting structure 21 needs to be thoroughly cleaned with pure water for better test effect, and pure water can be filled into the arc reflecting structure 21 through any one pipe to clean the arc reflecting structure 21, so that the arc reflecting structure can well realize specular reflection, and when the arc reflecting structure 21 is cleaned, the injected water cannot be excessive, otherwise, the culture medium can be cleaned, and the accuracy of the experiment is greatly affected.
The upper surface and the lower surface of the crystal oscillator 32 are both formed with foil-shaped excitation electrodes 322, the foil-shaped excitation electrodes 322 positioned on the upper surface of the crystal oscillator 32 are led out along the upper surface of the crystal oscillator 32 and are connected to the left side of the upper surface of the crystal oscillator support 31, the foil-shaped excitation electrodes 322 positioned on the lower surface of the crystal oscillator 32 are led out along the lower surface of the crystal oscillator 32 and are connected to the right side of the upper surface of the crystal oscillator support 31, and the left side and the right side of the upper surface of the crystal oscillator support 31 are respectively provided with a first metal contact 34 for being connected with an external circuit.
In the embodiment shown in fig. 1, the crystal oscillator device pressing component 5 further comprises at least two crystal oscillator device pressing components 5, the crystal oscillator device pressing components 5 comprise fixing portions 51 and pressing portions 52, the fixing portions 51 are fixed above the supporting table 2, and the pressing portions 52 are formed by bending the fixing portions 51 downwards to press and fix the crystal oscillator device 3.
As in the above embodiment, the pressing portion 52 includes a conductive wire (not shown) including a wire provided inside the pressing portion 52 and a second metal contact 53 provided on an end surface of the pressing portion 52, the second metal contact 53 facing the first metal contact 34, the second metal contact 53 being in good contact with the first metal contact 34.
In one embodiment, as shown in fig. 1, the solar simulation device further comprises a light filter 6, wherein the light filter 6 is arranged below the solar simulation device 4, and illumination with specific wavelengths can be filtered out through the light filter 6.
In one embodiment, as shown in fig. 1, the device further comprises a motor driving system 7, wherein the motor driving system 7 drives the sunlight simulating device 4 to rotate, so that the culture medium obtains uniform reflected illumination, the motor driving system is arranged above the upper cover 12, and an output shaft of the motor driving system passes through the upper cover in a sealing way and is connected with the sunlight simulating device 4.
In one embodiment, as shown in fig. 2, the base 11 and the upper cover 12 are both circular structures, and further include an air inlet channel 14 and an air outlet channel 15, where the air inlet channel 14 and the air outlet channel 15 are respectively disposed in tangential directions of the lower portion and the upper portion of the base 11, and the air inlet channel 14 and the air outlet channel 15 are disposed in opposite directions, and the air entering from the air inlet channel 14 forms a spiral upward air flow in the accommodating space 13, and is finally discharged from the air outlet channel 15. The temperature, humidity, gas composition, and gas pressure of the accommodating space 13 can be controlled through the gas inlet channel 14 and the gas outlet channel 15.
In the above embodiment, the inner wall of the base 11 may further be provided with a spiral air guide groove, where the lower end of the spiral air guide groove is connected to the air inlet channel 14, and the upper end of the spiral air guide groove is connected to the air outlet channel 15, so that the air flow will more easily form a spiral upward state under the action of the spiral air guide groove, and the original air in the accommodating space 13 is discharged.
In the embodiment shown in fig. 3 and 4, the crystal oscillator support 31 includes an inner ring structure 310, connection columns 311 and an outer ring structure 312, the connection columns 311 are uniformly arranged in the inner ring structure 310 and the outer ring structure 312, the hollow structure 33 is formed between each two connection columns 311, the inner side wall of the inner ring structure 310 is recessed inwards to form a circumferential groove 3101, and the crystal oscillator 32 is embedded in the circumferential groove 3101.
In one embodiment, as shown in fig. 3, the connection post 311 is of a transparent structure, and the connection post 311 of the transparent structure allows light to pass through more easily, thereby improving the light utilization rate.
In one embodiment, the pressing portion 52 is a silicone chuck structure, and the second metal contact 53 is located at the center of the silicone chuck structure, and when the fixing portion 51 is fixed above the support table 2, the silicone chuck structure presses the periphery of the first metal contact 34, and makes the second metal contact 53 well contact with the first metal contact 34.
Compared with the prior art, with reference to fig. 1-4, the detection device 100 for food microorganisms according to the present invention comprises the crystal oscillator device 3 and the sunlight simulation device 4, wherein a hollowed structure 33 is arranged between the crystal oscillator support 31 and the crystal oscillator 32, the lower surface of the crystal oscillator 32 is arranged at the focus of the arc-shaped reflecting structure 21, and the lower surface of the crystal oscillator 32 is provided with a culture medium, so that the microorganism detection under the conditions of aerobic, anaerobic and sunlight simulation can be performed only by immersing or coating the culture medium with a sample solution, and then placing the culture medium in the present invention.
The foregoing description of the preferred embodiments of the present invention is not intended to limit the scope of the claims, which follow, as defined in the claims.
Claims (3)
1. A detection device for food microorganisms, comprising:
the shell comprises a base and an upper cover, wherein the base and the upper cover jointly form an accommodating space, and the upper cover can be hermetically covered on the base;
the supporting table is arranged in the accommodating space, and an arc-shaped reflecting structure is arranged on the upper surface of the supporting table;
the crystal oscillator device comprises a crystal oscillator bracket and a crystal oscillator arranged on the crystal oscillator bracket, the crystal oscillator device is arranged on the supporting table, a hollowed-out structure is further arranged between the crystal oscillator bracket and the crystal oscillator, a filter lens is further inlaid on the hollowed-out structure, the lower surface of the crystal oscillator is positioned at the focus of the arc-shaped reflecting structure, and a culture medium is arranged on the lower surface of the crystal oscillator;
the liquid supply system comprises a sample solution supply pipe, a culture medium solution supply pipe, a discharge pipe, a four-way electromagnetic valve and a liquid supply main pipe, wherein the liquid supply main pipe penetrates through the bottom of the base and is connected to the center of the bottom of the arc-shaped reflecting structure, and the liquid supply main pipe is respectively communicated with the sample solution supply pipe, the culture medium solution supply pipe and the discharge pipe through the four-way electromagnetic valve;
the simulated sunlight device is arranged above the crystal oscillator device, and light emitted by the simulated sunlight device irradiates the arc-shaped reflecting structure through the filter and is focused on the culture medium on the lower surface of the crystal oscillator through the arc-shaped reflecting structure;
foil-shaped excitation electrodes are formed on the upper surface and the lower surface of the crystal oscillator, the foil-shaped excitation electrodes on the upper surface of the crystal oscillator are led out along the upper surface of the crystal oscillator and connected to the left side of the upper surface of the crystal oscillator support, the foil-shaped excitation electrodes on the lower surface of the crystal oscillator are led out along the lower surface of the crystal oscillator and connected to the right side of the upper surface of the crystal oscillator support, and the left side and the right side of the upper surface of the crystal oscillator support are respectively provided with a first metal contact for being connected with an external circuit;
the crystal oscillator device comprises a supporting table, at least two crystal oscillator device compressing components, a crystal oscillator device compressing component and a crystal oscillator device compressing component, wherein the crystal oscillator device compressing component comprises a fixing part and a compressing part, the fixing part is fixed above the supporting table, and the compressing part is formed by downwards bending the fixing part and is used for compressing and fixing the crystal oscillator device;
the pressing part comprises a conductive wire, the conductive wire comprises a wire arranged in the pressing part and a second metal contact arranged on the end face of the pressing part, and the second metal contact is opposite to the first metal contact;
the base and the upper cover are of circular structures and further comprise an air inlet channel and an air outlet channel, the air inlet channel and the air outlet channel are respectively arranged in tangential directions of the lower part and the upper part of the base, the air inlet channel and the air outlet channel are arranged in opposite directions, gas entering from the air inlet channel forms spiral upward air flow in the accommodating space, and finally the air is discharged from the air outlet channel;
the inner wall of the base can be provided with a spiral air guide groove, the lower end of the spiral air guide groove is connected with the air inlet channel, and the upper end of the spiral air guide groove is connected with the air outlet channel;
the crystal oscillator support comprises an inner ring structure, connecting columns and an outer ring structure, wherein the connecting columns are uniformly distributed in the inner ring structure and the outer ring structure, a hollowed-out structure is formed between every two connecting columns, the inner side wall of the inner ring structure is inwards recessed to form a circumferential groove, and the crystal oscillator is embedded in the circumferential groove;
the connecting column is of a transparent structure.
2. The apparatus for detecting microorganisms in food according to claim 1, further comprising a motor driving system, wherein an output shaft of the motor driving system is connected with the sunlight simulating apparatus through the upper cover in a sealing manner, and the motor driving system drives the sunlight simulating apparatus to rotate so that the culture medium obtains uniform reflected illumination.
3. The detecting device for food microorganisms according to claim 1, wherein the pressing portion is a silicone sucker structure, the second metal contact is located at the center of the silicone sucker structure, and when the fixing portion is fixed above the support table, the silicone sucker structure presses the periphery of the first metal contact and brings the second metal contact into good contact with the first metal contact.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711437357.6A CN107881087B (en) | 2017-12-26 | 2017-12-26 | Detection device for food microorganisms |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711437357.6A CN107881087B (en) | 2017-12-26 | 2017-12-26 | Detection device for food microorganisms |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107881087A CN107881087A (en) | 2018-04-06 |
| CN107881087B true CN107881087B (en) | 2023-08-08 |
Family
ID=61772582
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711437357.6A Active CN107881087B (en) | 2017-12-26 | 2017-12-26 | Detection device for food microorganisms |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107881087B (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2006029873A (en) * | 2004-07-13 | 2006-02-02 | Nippon Dempa Kogyo Co Ltd | Quartz sensor and sensing device |
| CN102192860A (en) * | 2010-03-10 | 2011-09-21 | 日本电波工业株式会社 | Method of detecting microorganisms and microorganism detecting apparatus |
| CN102453674A (en) * | 2010-10-15 | 2012-05-16 | 新奥科技发展有限公司 | Photobioreactor system |
| CN202626162U (en) * | 2012-04-10 | 2012-12-26 | 青岛浩澳环保科技有限公司 | Photoreactivation microorganism culture device |
| CN106010941A (en) * | 2016-07-19 | 2016-10-12 | 华测检测认证集团股份有限公司 | Culture apparatus for microbiological detection |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP4299325B2 (en) * | 2006-08-30 | 2009-07-22 | 日本電波工業株式会社 | Quartz sensor and sensing device |
-
2017
- 2017-12-26 CN CN201711437357.6A patent/CN107881087B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2006029873A (en) * | 2004-07-13 | 2006-02-02 | Nippon Dempa Kogyo Co Ltd | Quartz sensor and sensing device |
| CN102192860A (en) * | 2010-03-10 | 2011-09-21 | 日本电波工业株式会社 | Method of detecting microorganisms and microorganism detecting apparatus |
| CN102453674A (en) * | 2010-10-15 | 2012-05-16 | 新奥科技发展有限公司 | Photobioreactor system |
| CN202626162U (en) * | 2012-04-10 | 2012-12-26 | 青岛浩澳环保科技有限公司 | Photoreactivation microorganism culture device |
| CN106010941A (en) * | 2016-07-19 | 2016-10-12 | 华测检测认证集团股份有限公司 | Culture apparatus for microbiological detection |
Non-Patent Citations (1)
| Title |
|---|
| 文霞 ; 杨秀茳 ; 谢小保 ; .化妆品微生物检测技术的研究进展.日用化学工业.2015,第45卷(第02期),第110-114页. * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN107881087A (en) | 2018-04-06 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107868753B (en) | Detection device for cosmetic microorganism | |
| CN1283998A (en) | Surface and air sterilization using ultraviolet light and ultrasonic waves | |
| CN107881087B (en) | Detection device for food microorganisms | |
| WO2021129258A1 (en) | Auxiliary apparatus for use in intelligent elderly care service industry | |
| CN207775215U (en) | Detection device for Cosmetics microorganism | |
| CN213506931U (en) | Quick fermentation bottle equipment with functions of exhausting, stirring, heat preservation, temperature control and heating | |
| CN207958371U (en) | Microorganism detector | |
| CN207958327U (en) | Detection device for food microorganisms | |
| CN212282250U (en) | Comprehensive and thorough disinfection box | |
| CN213666975U (en) | Medical oncology nursing degassing unit | |
| CN207775243U (en) | The detection device of microorganism | |
| CN214283165U (en) | Heating sterilization device is used in soy sauce production | |
| CN112391276A (en) | Food microorganism detection device | |
| CN211068213U (en) | Environmental protection slippers disinfecting equipment | |
| CN202863823U (en) | Control equipment for mould produced in production process of bottled water | |
| CN213759658U (en) | Laboratory table surface disinfection and sterilization cover | |
| CN111558071A (en) | Comprehensive and thorough disinfection box | |
| CN208617813U (en) | Disinfector is used in a kind of production of bamboo wine | |
| CN218174953U (en) | Microorganism application of sample device | |
| CN215216627U (en) | Air sterilizer | |
| CN112902373B (en) | High-efficient air sterilizing apparatus | |
| CN215216628U (en) | Air sterilizer | |
| CN213245013U (en) | A bacterium reagent rack for medical science detects | |
| CN214973565U (en) | Vortex oscillator with sterilization function | |
| CN205268664U (en) | Double -purpose medical instrument disinfecting cabinet |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20231114 Address after: 266101 2, 3 storey factory building 3, No. 7, Gao Chang Road, Laoshan District, Qingdao, Shandong. Patentee after: QINGDAO HUACE DETECTION TECHNOLOGY CO.,LTD. Patentee after: CENTRE TESTING INTERNATIONAL GROUP Co.,Ltd. Address before: 518101 C building, Hongwei Industrial Park, 70 District, Guangdong, Shenzhen, Baoan District Patentee before: CENTRE TESTING INTERNATIONAL GROUP Co.,Ltd. |
|
| TR01 | Transfer of patent right |