CN107858293B - Talaromyces aurantiaca and application thereof - Google Patents
Talaromyces aurantiaca and application thereof Download PDFInfo
- Publication number
- CN107858293B CN107858293B CN201710946541.7A CN201710946541A CN107858293B CN 107858293 B CN107858293 B CN 107858293B CN 201710946541 A CN201710946541 A CN 201710946541A CN 107858293 B CN107858293 B CN 107858293B
- Authority
- CN
- China
- Prior art keywords
- talaromyces
- jxbr04
- phosphate
- strain
- culture medium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 241000228341 Talaromyces Species 0.000 title claims description 23
- 229910019142 PO4 Inorganic materials 0.000 claims abstract description 40
- 244000302661 Phyllostachys pubescens Species 0.000 claims abstract description 40
- 235000003570 Phyllostachys pubescens Nutrition 0.000 claims abstract description 40
- 239000010452 phosphate Substances 0.000 claims abstract description 29
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims abstract description 29
- 239000007788 liquid Substances 0.000 claims abstract description 24
- 241000857964 Ileodictyon cibarium Species 0.000 claims abstract description 17
- 230000012010 growth Effects 0.000 claims abstract description 16
- 239000002068 microbial inoculum Substances 0.000 claims abstract description 13
- 241000638881 Talaromyces aurantiacus Species 0.000 claims abstract description 8
- 238000004321 preservation Methods 0.000 claims abstract description 6
- 230000003248 secreting effect Effects 0.000 claims abstract description 4
- 235000021317 phosphate Nutrition 0.000 claims description 32
- 239000001963 growth medium Substances 0.000 claims description 24
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims description 18
- 239000011591 potassium Substances 0.000 claims description 18
- 229910052700 potassium Inorganic materials 0.000 claims description 18
- 238000009630 liquid culture Methods 0.000 claims description 14
- 238000012258 culturing Methods 0.000 claims description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 9
- 241000233866 Fungi Species 0.000 claims description 8
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 7
- 239000008103 glucose Substances 0.000 claims description 7
- 238000000034 method Methods 0.000 claims description 7
- 229920001817 Agar Polymers 0.000 claims description 6
- 244000061456 Solanum tuberosum Species 0.000 claims description 6
- 235000002595 Solanum tuberosum Nutrition 0.000 claims description 6
- 239000008272 agar Substances 0.000 claims description 6
- 239000012153 distilled water Substances 0.000 claims description 6
- 230000001737 promoting effect Effects 0.000 claims description 6
- ILRRQNADMUWWFW-UHFFFAOYSA-K aluminium phosphate Chemical compound O1[Al]2OP1(=O)O2 ILRRQNADMUWWFW-UHFFFAOYSA-K 0.000 claims description 5
- FUFJGUQYACFECW-UHFFFAOYSA-L calcium hydrogenphosphate Chemical compound [Ca+2].OP([O-])([O-])=O FUFJGUQYACFECW-UHFFFAOYSA-L 0.000 claims description 5
- 235000019700 dicalcium phosphate Nutrition 0.000 claims description 5
- WPEXVRDUEAJUGY-UHFFFAOYSA-B hexacalcium;(2,3,4,5,6-pentaphosphonatooxycyclohexyl) phosphate Chemical compound [Ca+2].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[O-]P([O-])(=O)OC1C(OP([O-])([O-])=O)C(OP([O-])([O-])=O)C(OP([O-])([O-])=O)C(OP([O-])([O-])=O)C1OP([O-])([O-])=O WPEXVRDUEAJUGY-UHFFFAOYSA-B 0.000 claims description 5
- 229910000398 iron phosphate Inorganic materials 0.000 claims description 5
- WBJZTOZJJYAKHQ-UHFFFAOYSA-K iron(3+) phosphate Chemical compound [Fe+3].[O-]P([O-])([O-])=O WBJZTOZJJYAKHQ-UHFFFAOYSA-K 0.000 claims description 5
- 239000007787 solid Substances 0.000 claims description 5
- 150000003013 phosphoric acid derivatives Chemical class 0.000 claims description 4
- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 claims description 4
- 229910000391 tricalcium phosphate Inorganic materials 0.000 claims description 4
- 239000001506 calcium phosphate Substances 0.000 claims description 3
- 235000011389 fruit/vegetable juice Nutrition 0.000 claims description 3
- 235000019731 tricalcium phosphate Nutrition 0.000 claims description 3
- 229940078499 tricalcium phosphate Drugs 0.000 claims description 3
- 229940001007 aluminium phosphate Drugs 0.000 claims description 2
- 229910000147 aluminium phosphate Inorganic materials 0.000 claims description 2
- 239000008188 pellet Substances 0.000 claims description 2
- 238000005063 solubilization Methods 0.000 claims description 2
- 230000007928 solubilization Effects 0.000 claims description 2
- FFRBMBIXVSCUFS-UHFFFAOYSA-N 2,4-dinitro-1-naphthol Chemical compound C1=CC=C2C(O)=C([N+]([O-])=O)C=C([N+]([O-])=O)C2=C1 FFRBMBIXVSCUFS-UHFFFAOYSA-N 0.000 claims 1
- 230000001580 bacterial effect Effects 0.000 claims 1
- 239000003795 chemical substances by application Substances 0.000 claims 1
- 230000001954 sterilising effect Effects 0.000 claims 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 abstract description 27
- 239000011574 phosphorus Substances 0.000 abstract description 27
- 229910052698 phosphorus Inorganic materials 0.000 abstract description 27
- 239000002689 soil Substances 0.000 abstract description 22
- 239000003337 fertilizer Substances 0.000 abstract description 9
- 235000015097 nutrients Nutrition 0.000 abstract description 6
- 230000000694 effects Effects 0.000 abstract description 5
- 238000006243 chemical reaction Methods 0.000 abstract 1
- 239000002131 composite material Substances 0.000 abstract 1
- 230000003381 solubilizing effect Effects 0.000 description 25
- 239000002609 medium Substances 0.000 description 18
- 244000005700 microbiome Species 0.000 description 13
- 241000196324 Embryophyta Species 0.000 description 10
- 239000011575 calcium Substances 0.000 description 10
- 241000894006 Bacteria Species 0.000 description 7
- 238000000855 fermentation Methods 0.000 description 7
- 230000004151 fermentation Effects 0.000 description 7
- 238000011081 inoculation Methods 0.000 description 7
- 229910017119 AlPO Inorganic materials 0.000 description 4
- 235000017166 Bambusa arundinacea Nutrition 0.000 description 4
- 235000017491 Bambusa tulda Nutrition 0.000 description 4
- 241001330002 Bambuseae Species 0.000 description 4
- 239000002028 Biomass Substances 0.000 description 4
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 4
- 235000015334 Phyllostachys viridis Nutrition 0.000 description 4
- 239000002253 acid Substances 0.000 description 4
- 230000002378 acidificating effect Effects 0.000 description 4
- 239000011425 bamboo Substances 0.000 description 4
- 238000011161 development Methods 0.000 description 4
- 238000004382 potting Methods 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- 230000028327 secretion Effects 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 241000486634 Bena Species 0.000 description 3
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- DLHONNLASJQAHX-UHFFFAOYSA-N aluminum;potassium;oxygen(2-);silicon(4+) Chemical compound [O-2].[O-2].[O-2].[O-2].[O-2].[O-2].[O-2].[O-2].[Al+3].[Si+4].[Si+4].[Si+4].[K+] DLHONNLASJQAHX-UHFFFAOYSA-N 0.000 description 3
- 238000005520 cutting process Methods 0.000 description 3
- 230000018109 developmental process Effects 0.000 description 3
- PCHPORCSPXIHLZ-UHFFFAOYSA-N diphenhydramine hydrochloride Chemical compound [Cl-].C=1C=CC=CC=1C(OCC[NH+](C)C)C1=CC=CC=C1 PCHPORCSPXIHLZ-UHFFFAOYSA-N 0.000 description 3
- 238000004090 dissolution Methods 0.000 description 3
- 239000004576 sand Substances 0.000 description 3
- 238000011282 treatment Methods 0.000 description 3
- 229910021578 Iron(III) chloride Inorganic materials 0.000 description 2
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 2
- 230000006518 acidic stress Effects 0.000 description 2
- 229910000323 aluminium silicate Inorganic materials 0.000 description 2
- 238000004737 colorimetric analysis Methods 0.000 description 2
- HNPSIPDUKPIQMN-UHFFFAOYSA-N dioxosilane;oxo(oxoalumanyloxy)alumane Chemical compound O=[Si]=O.O=[Al]O[Al]=O HNPSIPDUKPIQMN-UHFFFAOYSA-N 0.000 description 2
- 238000011049 filling Methods 0.000 description 2
- 229910052500 inorganic mineral Inorganic materials 0.000 description 2
- 230000006799 invasive growth in response to glucose limitation Effects 0.000 description 2
- RBTARNINKXHZNM-UHFFFAOYSA-K iron trichloride Chemical compound Cl[Fe](Cl)Cl RBTARNINKXHZNM-UHFFFAOYSA-K 0.000 description 2
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000011159 matrix material Substances 0.000 description 2
- 230000008635 plant growth Effects 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- 241000186361 Actinobacteria <class> Species 0.000 description 1
- 241001330024 Bambusoideae Species 0.000 description 1
- 241000123346 Chrysosporium Species 0.000 description 1
- IMQLKJBTEOYOSI-GPIVLXJGSA-N Inositol-hexakisphosphate Chemical compound OP(O)(=O)O[C@H]1[C@H](OP(O)(O)=O)[C@@H](OP(O)(O)=O)[C@H](OP(O)(O)=O)[C@H](OP(O)(O)=O)[C@@H]1OP(O)(O)=O IMQLKJBTEOYOSI-GPIVLXJGSA-N 0.000 description 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- 241000209504 Poaceae Species 0.000 description 1
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 description 1
- 241000207929 Scutellaria Species 0.000 description 1
- 238000012300 Sequence Analysis Methods 0.000 description 1
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- WYWFMUBFNXLFJK-UHFFFAOYSA-N [Mo].[Sb] Chemical compound [Mo].[Sb] WYWFMUBFNXLFJK-UHFFFAOYSA-N 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 229910052586 apatite Inorganic materials 0.000 description 1
- 238000010009 beating Methods 0.000 description 1
- 230000033558 biomineral tissue development Effects 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 description 1
- 229910000397 disodium phosphate Inorganic materials 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 235000020774 essential nutrients Nutrition 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 125000002791 glucosyl group Chemical group C1([C@H](O)[C@@H](O)[C@H](O)[C@H](O1)CO)* 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- 229910001629 magnesium chloride Inorganic materials 0.000 description 1
- WRUGWIBCXHJTDG-UHFFFAOYSA-L magnesium sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Mg+2].[O-]S([O-])(=O)=O WRUGWIBCXHJTDG-UHFFFAOYSA-L 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 239000010445 mica Substances 0.000 description 1
- 229910052618 mica group Inorganic materials 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- VSIIXMUUUJUKCM-UHFFFAOYSA-D pentacalcium;fluoride;triphosphate Chemical compound [F-].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O VSIIXMUUUJUKCM-UHFFFAOYSA-D 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 235000002949 phytic acid Nutrition 0.000 description 1
- 230000008121 plant development Effects 0.000 description 1
- 230000001863 plant nutrition Effects 0.000 description 1
- 229940072033 potash Drugs 0.000 description 1
- BWHMMNNQKKPAPP-UHFFFAOYSA-L potassium carbonate Substances [K+].[K+].[O-]C([O-])=O BWHMMNNQKKPAPP-UHFFFAOYSA-L 0.000 description 1
- 235000015320 potassium carbonate Nutrition 0.000 description 1
- 239000012286 potassium permanganate Substances 0.000 description 1
- 229940070017 potassium supplement Drugs 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000001243 protein synthesis Methods 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000010298 pulverizing process Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 239000010703 silicon Substances 0.000 description 1
- 229910052710 silicon Inorganic materials 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- 238000009331 sowing Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 229960004793 sucrose Drugs 0.000 description 1
- 230000031068 symbiosis, encompassing mutualism through parasitism Effects 0.000 description 1
- 239000008399 tap water Substances 0.000 description 1
- 235000020679 tap water Nutrition 0.000 description 1
- QSUVHYRUWBKUKW-UHFFFAOYSA-H tetracalcium;diphosphate Chemical compound [Ca+2].[Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QSUVHYRUWBKUKW-UHFFFAOYSA-H 0.000 description 1
- 230000014616 translation Effects 0.000 description 1
- 239000010455 vermiculite Substances 0.000 description 1
- 229910052902 vermiculite Inorganic materials 0.000 description 1
- 235000019354 vermiculite Nutrition 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/145—Fungal isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Mycology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Virology (AREA)
- Pest Control & Pesticides (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biomedical Technology (AREA)
- Botany (AREA)
- Agronomy & Crop Science (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Environmental Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Fertilizers (AREA)
Abstract
The invention discloses a golden basket fungus, which is classified and named as golden basket fungus (a)Talaromyces aurantiacus) The strain number is JXBR04, the strain is preserved in China center for type culture Collection, and the preservation number is CCTCC NO: m2017327, the preservation date is 6 months and 13 days in 2017. The golden basket fungus screened from the rhizosphere soil of the moso bamboo not only has a high-efficiency phosphate-solubilizing function, but also has better potassium-solubilizing and IAA-secreting capabilities. Under the condition of liquid shake culture, the composite fertilizer has stronger dissolving capacity on insoluble phosphate and promotes the conversion of insoluble phosphorus into soluble nutrients; the invention also discloses application of the golden basket fungus in growth promotion of moso bamboos, and the liquid microbial inoculum prepared by the golden basket fungus has remarkable growth promotion effect on the ground diameter and seedling height of the moso bamboos, so that the invention provides excellent strain resources for developing environment-friendly fertilizer special for moso bamboos.
Description
Technical Field
The invention relates to the field of microorganisms and biofertilizers, and relates to a golden basket fungus and application thereof.
Background
Phyllostachys edulis (Phyllostachys edulis) is an economic forest of Bambusoideae of Gramineae. According to incomplete statistics, the existing moso bamboo forest in China exceeds 380 kiloha, and accounts for 70% of the area of the bamboo forest in China and 80% of the area of the moso bamboo forest in the whole world. In many mountainous areas in the south of China, moso bamboos become main economic forest species for structure adjustment of agriculture and forestry industries, efficiency improvement of forestry and income increase of forest people, however, the current moso bamboo forest management is mainly extensive, long-term unreasonable management causes land weakness of the moso bamboo forest and insufficient nutrient supply, especially lack of phosphorus, and the moso bamboo forest land is one of the main problems in moso bamboo resource cultivation; the fertilizer can obviously promote the growth of the artificial phosphorus of the moso bamboo, however, the negative effect is increasingly obvious along with the global energy crisis and the rapid increase of the using amount of the fertilizer. Therefore, research into finding new fertilizers, particularly biomass fertilizers, to replace or partially replace fertilizers has received much attention. Moso bamboo forests are often distributed in hilly red soil areas deficient in phosphorus, and recent studies have shown that phosphorus has become the primary factor limiting the productivity of moso bamboo. Phosphorus is one of the essential nutrient elements for the growth and development of moso bamboos and biomass accumulation. Over 95 percent of phosphorus in the red soil is difficult to be directly absorbed and utilized by plants, and the global environment changes, thus aggravating the unavailability of the phosphorus. The yield of bamboo and bamboo shoots is in a descending trend year by year due to the lack of phosphorus in soil, and the continuous operation and utilization of the moso bamboo forest are seriously threatened. In addition, potassium is one of three elements of plant nutrition, and plays an important role in physiological processes such as sugar metabolism, protein synthesis, plant resistance enhancement and the like. However, about 60% of cultivated land in China is lack of potassium, 95% of potassium in soil is in the form of mineral potassium, potassium exists in potassium feldspar and mica, potassium which can be absorbed and utilized by plants is not more than 2% of total potassium, and the development of agriculture and forestry is influenced. At present, the potassium supplement by using chemical potash fertilizer is not only high in cost, but also causes environmental problems such as soil structure damage, organic matter content reduction and the like.
Rhizosphere microorganisms are key players and regulators of rhizosphere processes. Rhizosphere soil microorganisms directly or indirectly influence plant community structure and productivity by changing the effectiveness of soil nutrients and the way of symbiosis with plant roots. As early as the beginning of the 20 th century, attention has been paid to the relationship between microorganisms and soil phosphorus. Sackett (1908) for the first time discovered that some originally insoluble phosphates and natural phosphate ores could be used by some bacteria. Since then, studies for promoting plant growth and improving plant phosphorus nutrition by utilizing the dissolution of insoluble phosphorus by rhizosphere microorganisms have been widely conducted.
Research shows that a plurality of microorganisms with the capability of converting insoluble phosphorus and potassium into plant available phosphorus and potassium exist in soil microorganisms, and the microorganisms are called as phosphate solubilizing microorganisms and potassium solubilizing microorganisms. Phosphate solubilizing microorganisms play an important role in the biochemical cycle of soil P, and they can convert inorganic phosphorus and organic phosphorus which are difficult to dissolve in soil into available phosphorus through dissolution and mineralization, and the available phosphorus is absorbed and utilized by plants (whitleraw et al, 1999). According to incomplete statistics, at present, 89 phosphate solubilizing microorganisms including phosphate solubilizing fungi, phosphate solubilizing bacteria, phosphate solubilizing actinomycetes and the like are screened out worldwide. Although the number and the types of the phosphate Solubilizing fungi (Solubilizing phosphorus fungi) are less than those of phosphate Solubilizing bacteria, research shows that the phosphate Solubilizing fungi are generally stronger in phosphate Solubilizing capability than the bacteria, sometimes even dozens of times of the bacteria, and the genetic characters are more stable. The potassium-dissolving bacteria can decompose insoluble aluminosilicate inorganic mineral substances such as potassium feldspar, apatite and the like, promote nutrient elements such as insoluble potassium, phosphorus, silicon, magnesium and the like to be converted into soluble nutrients, increase the content of available nutrients in soil, promote plant growth and development and improve yield. Therefore, the phosphate-solubilizing and potassium-solubilizing fungi which are used as an important microorganism in soil phosphorus circulation can improve the supply of phosphorus and potassium of the moso bamboos and enable the moso bamboos to grow faster and better. At present, no report is found about the screening and application research of functional strains with the functions of phosphate and potassium dissolving and IAA secretion of phyllostachys pubescens rhizosphere.
Disclosure of Invention
In order to overcome the defects of the prior art, the invention aims to provide the golden bluefungus for promoting the growth of moso bamboos, and the dissolving capacity and IAA secretion capacity of insoluble phosphate and aluminosilicate in soil.
The invention also aims to solve the technical problem of providing the application of the above golden basket fungus in promoting the growth of moso bamboos.
In order to solve the technical problems, the technical scheme adopted by the invention is as follows:
a strain of efficient phosphate-solubilizing Talaromyces aurantiacas (strain number JXBR 04) is obtained by screening rhizosphere soil of 5-year-old Mao bamboo grown in a large-port forest farm in Yifeng county, Yichun city, Jiangxi province. Has been preserved in China Center for Type Culture Collection (CCTCC) with the address: wuhan university in Wuhan City, Hubei province, China, zip code: 430072, with the preservation number: CCTCC NO: m2017327, the preservation date is 6 months and 13 days in 2017.
The main biological characteristics of the golden basket fungus (Talaromyces aurantiacaus) JXBR04 are as follows: the colony on a Czapek Yeast Extract Agar medium (CYA) plate is round, and the front side of the colony is yellow; conidiophores, glimmer-shaped branches, spherical spores and single cells.
The sequences of the golden basket fungus (Talaromyces aurantiacus) IST, BenA and CaM are shown in SEQ ID No: 1 is shown. And comparing and analyzing the sequences of the ITS, the BenA and the CaM with the sequences in a GeneBank database. The results show that the strain JXBR04 has high homology with Talaromyces (Talaromyces) and has 100 percent of mutual recognition with Talaromyces aurantiacaus. Combined with the ITS, BenA and CaM sequence analysis, the strain is identified as Talaromyces aurantiacas.
The JXBR04 strain can convert insoluble inorganic phosphorus in soil into soluble phosphorus for the moso bamboo to absorb and utilize, thereby promoting the growth of the moso bamboo.
The invention also provides a preparation method of the liquid microbial inoculum, which comprises the following specific operations:
1) inoculating the strain JXBR04 of the golden basket fungus (Talaromyces aurantiacus) to a PDA solid culture medium,
2) after the plate is full of hyphae, perforating the plate with mycelium of JXBR04 of Talaromyces aurantiacas (Talaromyces aurantiacas) with an aseptic perforator,
3) and then, transferring the mycelium cake into a 250ml triangular flask filled with 150ml PDA liquid culture medium by using a needle for propagation, inoculating 10 blocks into each flask, and culturing for 6-8 days at 25 ℃ and 121r/min until the mycelium pellet grows full of the liquid culture medium, namely the liquid microbial inoculum.
Further, the diameter d of the sterile punch is 7 mm.
Further, the air conditioner is provided with a fan,in step 3), before propagation, the PDA liquid culture medium is treated by 1.01 multiplied by 106Sterilizing at Pa and 121 deg.C for 20min, and keeping aseptic state.
Has the advantages that: compared with the prior art, the Talaromyces aurantiacas has the high-efficiency phosphate solubilizing function and also has better potassium solubilizing and IAA secreting capabilities. For insoluble tricalcium phosphate (Ca) under indoor liquid culture conditions3(PO4)2) Iron phosphate (FePO)4) Calcium hydrogen phosphate (CaHPO)4) Aluminum phosphate (AlPO)4) And calcium phytate (organic phytate) have strong dissolving effect; the rhizosphere efficient phosphate-solubilizing golden basket fungus of the moso bamboo is prepared into a liquid microbial inoculum to inoculate annual moso bamboo seedlings. The result shows that the microbial inoculum can obviously promote the growth and development of the moso bamboo, so that the invention provides excellent strain resources for developing the microbial fertilizer special for the moso bamboo in the future.
Drawings
FIG. 1 shows the colony morphology of Sciadophyllum chrysosporium JXBR04 on CYA medium and the phosphorus-solubilizing circles generated on NBRIP medium, Panel A: culturing the basket fungus on CYA culture medium for 7 days to obtain colony morphology; and B, drawing: p-lysing rings were generated by culturing the Scutellaria aurantiaca in NBRIP medium for 7 days.
FIG. 2 shows conidiophores and conidia of the basket fungus, and broom-like molecular conidiophores and elliptical molecular spore forms can be observed from the left, middle and right panels.
FIG. 3 is a phylogenetic tree of the CaM gene sequence of strain JXBR 04.
FIG. 4 shows that strain JXBR04 was used to treat 5 types of calcium phosphate tricalcium phosphate (Ca) with poor solubility under different pH conditions3(PO4)2) Iron phosphate (FePO)4) Calcium hydrogen phosphate (CaHPO)4) Aluminum phosphate (AlPO)4) And the dissolution capacity of calcium phytate.
FIG. 5 shows the potassium-solubilizing ability of strain JXBR 04.
FIG. 6 shows the IAA secretion ability of JXBR 04.
Detailed Description
The present invention will be better understood by those skilled in the art from the following examples. The examples are described only to illustrate the invention and should not be construed as limiting the invention as detailed in the claims.
Example 1: growth test of JXBR04 Strain under acid stress
Activated strain JXBR04 is inoculated into PDA liquid culture medium (the formula of the PDA liquid culture medium is that potato 200g, glucose 20g and distilled water 1000mL), shaking culture is carried out at 25 ℃ and 120r/min for 5d to serve as seed liquid, the pH of the PDA liquid culture medium is set to be 1.5, 2.5, 3.5, 4.5, 5.5 and 6.5, the seed liquid is divided into 250mL triangular flasks, and the liquid filling amount is 100 mL. Subsequently, the seed solutions were inoculated into PDA liquid medium in an inoculum size of 1% (v/v), and the treatment was repeated 3 times, and shaking culture was carried out at 25 ℃ and 120r/min for 8 d. Filtering the mycelium, drying at 65 ℃ for 48h, and weighing. The results are shown in Table 1, the strain JXBR04 grows better under different acidic conditions of pH 1.5, 2.5, 3.5, 4.5, 5.5 and 6.5, the hypha biomass is not different, and the pH range of the fermentation liquor is 3.40-3.95. The results show that the strain JXBR04 can regulate the self-appropriate growth acid environment by secreting acid substances, is further suitable for different acid environments, and has the ability of growing in different acid soil environments.
TABLE 1 Effect of different pH on JXBR04 hyphal biomass and broth pH
Note: p is less than 0.05, and the small and the letter of different rows in the same column represent the difference
Example 2: strain JXBR04 was tested for phosphate solubilization under acid stress.
Phosphate solubilizing medium a: glucose 10g, Ca3(PO4)2 5g，MgCl2 5g，KCl 0.2g，MgSO4.7H2O 0.25g，(NH4)28O40.1g, and 1000mL of distilled water.
Phosphate solubilizing medium B: with aluminium phosphate (AlPO)4) Replace Ca in the phosphate solubilizing medium A3(PO4)3Other ingredientsAnd the content is the same.
Phosphate solubilizing medium C: from iron phosphate (FePO)4) Replace Ca in the phosphate solubilizing medium A3(PO4)3And other components and contents are the same.
Phosphate solubilizing medium D: with calcium hydrogen phosphate (CaHPO)4) Replace Ca in the phosphate solubilizing medium A3(PO4)3And other components and contents are the same.
Phosphate solubilizing medium E: with calcium phytate (C)6H6Ca6O24P6) Replace Ca in the phosphate solubilizing medium A3(PO4)3And other components and contents are the same.
Inoculating activated JXBR04 strain into PDA culture medium (the formula of PDA culture medium is 200g of potato, 20g of glucose, 18g of agar and 1000mL of distilled water), culturing with shaking at 25 ℃ and 120r/min for 5d to serve as seed liquid, respectively inoculating the seed liquid into 100mL triangular flasks containing 50mL of phosphate solubilizing culture medium A, B, C, D and E with the inoculation amount of 1% (v/v) and the pH value respectively adjusted to 1.5, 2.5, 3.5, 4.5, 5.5 and 6.5, respectively, treating the phosphate solubilizing culture medium with the same volume of blank seed liquid as Control (CK) for three times, culturing at 25 ℃ and 121r/min for 8d, centrifuging the fermentation liquid at 4 ℃, 10000r/min for 10min, determining the content of soluble phosphorus in the fermentation liquid by molybdenum-antimony colorimetric method,
the results are shown in FIG. 4. As can be seen from FIG. 4, tricalcium phosphate (Ca), a sparingly soluble phosphate, was used3(PO4)2) Iron phosphate (FePO)4) Calcium hydrogen phosphate (CaHPO)4) Aluminum phosphate (AlPO)4) And calcium phytate (C)6H6Ca6O24P6) As the only phosphorus source, after the strain is cultured for 8 days in different acidic environments with pH values of 1.5, 2.5, 3.5, 4.5, 5.5 and 6.5, the content of soluble phosphorus in the JXBR04 fermentation liquor is obviously higher than that of CK in different acidic environments. The results show that the strain JXBR04 has strong dissolving capacity on five indissolvable phosphates under different acidic conditions.
Example 3: JXBR04 strain potassium-dissolving capacity determination test.
The JXBR04 strain is inoculated into a potassium-dissolving culture medium (potassium-dissolving culture) containing 50mLThe formula of the base is as follows: potassium feldspar powder 0.1g, Na2HPO4 2.0g，FeCl3 0.005g，MgSO4·7H20.5g of O, 5.0g of cane sugar and Ca2CO30.1g, agar 18.0g, distilled water 1000mL, pH 7.0. ) Performing shake culture for 7d at 28 deg.C and 180r/min in 100mL triangular flask to obtain fermentation liquid, removing insoluble substances from the fermentation liquid at 500r/min for 10min, centrifuging at 10000r/min for 10min to collect supernatant, and measuring the content of effective potassium in the supernatant by flame spectrophotometer. As shown in FIG. 5, JXBR04 showed a potassium-releasing amount of 15.28mg/L, which was superior in potassium-releasing ability.
Example 4: strain JXBR04 was tested for IAA-producing ability.
The IAA production capacity was determined by Salkowski colorimetry. Taking IAA standard, configuring into concentration gradient of 0, 0.5, 2.5, 5.0, 7.5, 10, 12.5, 15.0, 17.5, 25.0 and 50.0mg/L, taking 2mL of IAA with each concentration, adding equal amount of ferric chloride colorimetric solution (PC colorimetric solution), keeping the temperature in the dark at 30 ℃ for 30min, measuring absorbance at the wavelength of 530nm by using a spectrophotometer, drawing a standard curve, and obtaining the equation y which is 31.868x (R)20.9947). The activated strain JXBR04 was inoculated into King's medium (formula: peptone 2g glycerol 1g, K)25O4 0.15g、MgSO4 4.7g、H20.15g of O, 1.5g of agar and H2O1000 ml, pH 7.2) and shake culturing for 15d, and measuring the IAA content in the fermentation liquor according to the standard curve making method. As shown in FIG. 6, JXBR04 has better IAA production capacity, and the IAA secretion amount is 21.25 mg/L.
Example 5: JXBR04 greenhouse potting test:
(1) cultivation of moso bamboo bud seedlings
Soaking moso bamboo seeds in 0.5% potassium permanganate solution for 2h for surface disinfection, washing with tap water for 1h, sowing in sterilized sand, culturing in a greenhouse at 25 deg.C, and cutting bud seedling and transplanting when the moso bamboo seeds grow to cotyledon stage.
(2) The potting matrix soil is collected from the hind hill of the university of Jiangxi agriculture, sterilized at 165 ℃ for 2h according to the mass ratio of soil, sand and vermiculite of 2: 1, and cooled for later use.
(3) Preparation of liquid microbial inoculum
Inoculating the stored golden basket fungus strain into PDA solid culture medium (the formula of PDA solid culture medium is glucose 20g, potato juice 200g, agar 18g, water 1000mL), culturing at 25 deg.C for 6d, making the hypha overgrow the plate, beating the colony edge into fungus cake with a punch (d is 7mm) under aseptic condition, and transferring into 500mL triangular flask containing 250mL of PDA liquid culture medium (the formula of PDA liquid culture medium is potato 200g, glucose 20g, distilled water 1000mL) for propagation (the PDA liquid culture medium is subjected to 1.01 × 106Sterilizing at Pa and 121 deg.C for 20min, and keeping aseptic state); 10 fungus cakes were picked per bottle. Placing at a culture temperature of 25 ℃ and a culture rotation speed of 120 r.min-1Culturing under the culture condition until the mycelium is in a vigorous stage, and pulverizing with a sterilized homogenizer to obtain the liquid microbial inoculum.
(4) Potted plant test by liquid microbial inoculum inoculation
Transplanting the moso bamboo seedlings by adopting a bud seedling root cutting method, wherein the inoculation amount of the microbial inoculum is 10ml per plant. The specific method comprises the following steps: taking a proper amount of sterile potting medium, filling the potting medium into a flowerpot, gently taking out the moso bamboo seedlings in the cotyledon period from a seedling pot, preventing the roots of the moso bamboo seedlings from being damaged as far as possible, washing sand at the roots of the seedlings with clear water, cutting off a small amount of main roots of the seedlings by using a scalpel, transplanting the seedlings into the flowerpot, putting a microbial inoculum around the moso bamboo rhizosphere, compacting soil, covering with matrix soil, watering and fixing the roots. The control was made of two treatments, inoculation of liquid medium only and no inoculation of bacteria, 10 replicates for each treatment. Culturing in 25 deg.C greenhouse, watering at proper time, and managing uniformly. The growth condition of 180d after moso bamboo inoculation is shown in table 2, and it can be seen that the growth of moso bamboo seedlings can be remarkably promoted by the inoculation of JXBR 04. Compared with a Control (CK), the growth rate of the inoculated JXBR04 on the height of the seedlings and the stem thickness respectively reaches 30.95 percent and 28.57 percent, and the growth promoting effect is obvious.
TABLE 2 Effect of Strain JXBR04 on the growth of Phyllostachys Pubescens seedlings
Note: p is less than 0.05, and the lower case letters of different rows in the same column are different to represent difference display
Sequence listing
<110> university of agriculture in Jiangxi
<120> Talaromyces aurantiaca and application thereof
<130> 2017
<160> 3
<170> SIPOSequenceListing 1.0
<210> 1
<211> 562
<212> DNA
<213> Talaromyces aurantiacaus (Talaromyces aurantiacaus)
<400> 1
tttccgtagg tgaacctgcg gaaggatcat taccgagtgc gggccctcgc ggcccaacct 60
cccacccgtg tctctctcta cctgttgctt tggcgggccc accggggcca cccggtcgcc 120
gggggacgtc cgtctccggg cccgcgcccg ccgaagcgcc ctgtgaaccc tgatgaagat 180
gggctgtctg agtcgcatga aaattgtcaa aactttcaac aatggatctc ttggttccgg 240
catcgatgaa gaacgcagcg aaatgcgata agtaatgtga attgcagaat tccgtgaatc 300
atcgaatctt tgaacgcaca ttgcgccccc tggcattccg gggggcatgc ctgtccgagc 360
gtcatttctg ccctcaagcg cggcttgtgt gttgggcgtg gtccccccgg ggacctgccc 420
gaaaggcagc ggcgacgtcc gtccggtcct cgagcgtatg gggctctgtc actcgctcgg 480
gacggatcgg cggaggttgg tcaccaccac agttttacca cggttgacct cggatcaggt 540
aggagttacc cgctgaactt aa 562
<210> 2
<211> 415
<212> DNA
<213> Talaromyces aurantiacaus (Talaromyces aurantiacaus)
<400> 2
tggtgtgtaa aaagactcgg tcaattgtcg ccacaaacaa gctgactttt ccaggcaaat 60
catctctgct gagcacggtc tcgatggctc cggtgtgtaa gtgttgcaaa cgattcgaat 120
gcagttataa tccgacacca tctgatcatc aacagctaca atggctcctc cgacctccag 180
ttggagcgta tgaacgtcta cttcaacgag gtgtgtggaa tcaaccatca gaaaacccat 240
cgaatgcttg gaactcatgt ctcgaatata ggcctccggc aacaagtacg ttccccgtgc 300
cgtcctcgtc gacttggagc ccggtaccat ggatgccgtc cgcgctggtc cctttggtca 360
gctcttccgt cccgacaact ttgttttcgg tcaatccggt gccggtaaca actgg 415
<210> 3
<211> 662
<212> DNA
<213> Talaromyces aurantiacaus (Talaromyces aurantiacaus)
<400> 3
aagtctccga gtacaaggag gctttctctc tttttgtaag tctgaaatat ctccctgtcg 60
catactattt tctccttgct tacagaatgt ttttatgaaa taggacaagg atggagatgg 120
tgagtgacga ccgcgaccca ccgacgatgt cgtatcaaaa gcagattcat ctacgattta 180
cgaatgaata ttgatagagt cgaacaggtc aaatcacaac caaggaactt ggcaccgtca 240
tgcgctccct cggccagaac ccctccgaat ccgaattgca ggacatgatc aacgaagttg 300
acgctgacaa caacggcaca attgatttcc ctggtacgat catcacgcct caagctgctt 360
tcaatggaaa aactgaccgc cgcagaattc ttgaccatga tggcccgcaa aatgaaggat 420
accgactccg aggaagagat ccgcgaggcc ttcaaggtgt tcgaccgcga caacaacgga 480
ttcatctccg ccgccgaact gcgtcacgtt atgacctcga ttggcgagaa gctgactgac 540
gatgaggttg acgagatgat ccgcgaggct gaccaggacg gtgatggaag aatcgactgt 600
aagtgcttcc gtgtcctcca cgagataaga gaccggtcta actgttttct tttttagaca 660
ac 662
Claims (10)
1. A fungus, JINHUANGBASIU, is classified and named as JINHUANGBASIU (A)Talaromyces aurantiacus) The strain number is JXBR04, the strain is preserved in China center for type culture Collection, and the preservation number is: CCTCC NO: m2017327, the preservation date is 6 months and 13 days in 2017.
2. Use of the golden basket fungus of claim 1 for promoting the growth of moso bamboos.
3. The use of claim 2, wherein the Talaromyces aurantiaca (A), (B), (C), (Talaromyces aurantiacus) Use of JXBR04 for the solubilization of poorly soluble phosphates.
4. The use of claim 3, wherein the Talaromyces aurantiaca (A), (B), (C), (Talaromyces aurantiacus) JXBR04 dissolves insoluble phosphate in a gradient manner at a pH of 1.5-6.5.
5. Use according to claim 3, wherein the poorly soluble phosphates comprise tricalcium phosphate, iron phosphate, aluminium phosphate, calcium hydrogen phosphate, calcium phytate.
6. The use of claim 2, wherein the Talaromyces aurantiaca (A), (B), (C), (Talaromyces aurantiacus) Use of JXBR04 for dissolving poorly soluble potassium.
7. The use of claim 2, wherein the Talaromyces aurantiaca (A), (B), (C), (Talaromyces aurantiacus) JXBR04 has the function of secreting IAA.
8. The method for preparing the liquid microbial inoculum by utilizing the golden basket fungus of claim 1, which comprises the following steps:
1) the above-mentioned Talaromyces aureobasicola (A), (B), (CTalaromyces aurantiacus) JXBR04 strain is inoculated on PDA solid culture medium,
2) after the hyphae grow over the plate, the basket fungus with golden yellow is put in an aseptic puncherTalaromyces aurantiacus) Plates of JXBR04 hyphae were perforated,
3) and then, transferring the mycelium cake into a 250ml triangular flask filled with 150ml PDA liquid culture medium by using a needle for propagation, inoculating 10 blocks into each flask, and culturing for 6-8 days at 25 ℃ and 121r/min until the mycelium pellet grows full of the liquid culture medium, namely the liquid microbial inoculum.
9. The method for preparing liquid bacterial agent according to claim 8, wherein in step 3), PDA liquid culture medium is passed through 1.01 x 10 before propagation6 Sterilizing at Pa and 121 ℃ for 20 min.
10. The method for preparing liquid microbial inoculum according to claim 8, wherein the PDA solid culture medium has the following formula: 20g of glucose, 200g of potato juice, 18g of agar and 1000mL of water; the PDA liquid culture medium comprises the following components: 200g of potato juice, 20g of glucose and 1000mL of distilled water.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710946541.7A CN107858293B (en) | 2017-10-11 | 2017-10-11 | Talaromyces aurantiaca and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710946541.7A CN107858293B (en) | 2017-10-11 | 2017-10-11 | Talaromyces aurantiaca and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107858293A CN107858293A (en) | 2018-03-30 |
| CN107858293B true CN107858293B (en) | 2020-11-24 |
Family
ID=61698400
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710946541.7A Expired - Fee Related CN107858293B (en) | 2017-10-11 | 2017-10-11 | Talaromyces aurantiaca and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107858293B (en) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110257263B (en) * | 2019-07-24 | 2020-06-26 | 黑龙江省森林保护研究所 | Pinus sylvestris SBS-ZGTP02 and application thereof |
| CN112342144B (en) * | 2020-09-28 | 2023-02-14 | 江西农业大学 | Aspergillus flavus strain and application thereof |
| CN112812977B (en) * | 2021-03-08 | 2023-03-24 | 哈尔滨师范大学 | Phosphorus-dissolving fungus and application thereof |
| CN113717860B (en) * | 2021-07-07 | 2023-05-16 | 昆明理工大学 | Application of Huang Lanzhuang bacteria in conversion of total saponins of Notoginseng radix into small-polarity ginsenoside |
| CN115369043B (en) * | 2021-12-14 | 2023-06-16 | 贵州民族大学 | Multifunctional basket strain GYDW-YM101 and application thereof |
| CN114196550B (en) * | 2021-12-20 | 2024-04-26 | 安徽粤智徽源生物科技有限公司 | Monascus aureobasidiomycetes and method for producing haematochrome by utilizing same through illumination-stimulated fermentation |
| CN117535156B (en) * | 2022-12-01 | 2024-05-17 | 河北省科学院生物研究所 | Brucella CFT-1 and application thereof |
| CN116904321A (en) * | 2023-06-25 | 2023-10-20 | 黑龙江省科学院微生物研究所 | Basket fungus W10 and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102250784A (en) * | 2011-04-25 | 2011-11-23 | 南京中医药大学 | Angelica root rhizosphere efficient phosphate-solubilizing bacterium and microbial inoculum prepared from same and application thereof |
| CN103946369A (en) * | 2011-09-22 | 2014-07-23 | 丹尼斯科美国公司 | Endogenous dnase activity to reduce DNA content |
| CN104284588A (en) * | 2012-03-16 | 2015-01-14 | 露华浓 | Probiotic/antioxidant blend |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8551751B2 (en) * | 2007-09-07 | 2013-10-08 | Dyadic International, Inc. | BX11 enzymes having xylosidase activity |
| DK2976423T3 (en) * | 2013-03-21 | 2019-03-18 | Novozymes As | Polypeptides with phospholipase A activity and polynucleotides encoding them |
-
2017
- 2017-10-11 CN CN201710946541.7A patent/CN107858293B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102250784A (en) * | 2011-04-25 | 2011-11-23 | 南京中医药大学 | Angelica root rhizosphere efficient phosphate-solubilizing bacterium and microbial inoculum prepared from same and application thereof |
| CN103946369A (en) * | 2011-09-22 | 2014-07-23 | 丹尼斯科美国公司 | Endogenous dnase activity to reduce DNA content |
| CN104284588A (en) * | 2012-03-16 | 2015-01-14 | 露华浓 | Probiotic/antioxidant blend |
Also Published As
| Publication number | Publication date |
|---|---|
| CN107858293A (en) | 2018-03-30 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107858293B (en) | Talaromyces aurantiaca and application thereof | |
| CN105296363B (en) | A kind of penicillium oxalicum NJDL-03 bacterial strain and its application | |
| CN111117910B (en) | Enterobacter ludwigii PN6 and application thereof | |
| CN101974438B (en) | Eucalyptus endophyte and application thereof | |
| CN106085925B (en) | A kind of salt tolerant bacterium T5S2 and its application in microbial manure | |
| CN105936881B (en) | One kind is for alignic thermophilic sugared bacillus and its application method of degrading | |
| US11097991B1 (en) | Phosphorus fertilizer bio-catalyst for sustainable crop production | |
| CN104371945B (en) | Citrobacter freundii with phosphorus-dissolving potassium-dissolving capability and application thereof | |
| CN105964680B (en) | A kind of beach saline land continuous cropping cotton soil ecology renovation agent and the preparation method and application thereof | |
| CN103060242B (en) | Bacillus fusiformis for promoting rhizosphere growth of blueberries and application thereof | |
| CN101899409B (en) | Taxus chinensis var. mairei rhizosphere high-efficient phosphate-solubilizing pseudomonas fluorescens and application thereof | |
| CN104651237A (en) | Efficient phosphorus-solubilizing aspergillus niger strain and application thereof | |
| CN104371946A (en) | Pseudomonas vranovensis with phosphorus solubilizing capability and application thereof | |
| CN106518535A (en) | Full-nutrition ecological substrate nutrient soil and preparation method | |
| CN109280628B (en) | Enterobacter strain and application thereof in promoting growth of moso bamboos | |
| CN112342144B (en) | Aspergillus flavus strain and application thereof | |
| CN103045500B (en) | Mesorhizobium KDRM295 and application thereof | |
| CN110951625B (en) | Penicillium phosphate solubilizing PSF and application thereof | |
| CN111394255B (en) | Aspergillus buried and application thereof | |
| CN109112072A (en) | A kind of Trichoderma is promoting the application in plant growth | |
| CN108624512B (en) | Solid fermentation substrate, preparation method and method for culturing mycorrhiza biological preparation | |
| CN110229757A (en) | One plant effectively facilitates the tangerine green trichoderma JS84 of plant growth and its biological organic fertilizer of development | |
| CN102876598A (en) | Pseudomonas fluorescens and application of pseudomonas fluorescens for promoting growth of Chinese sweetgum | |
| CN112358978B (en) | Aspergillus and application thereof | |
| AU2021104551A4 (en) | Enterobacter ludwigii PN6 and its application |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20201124 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |