CN107857792A - A kind of extracting method of gleditschiasaponin - Google Patents

Abstract

A kind of extracting method of gleditschiasaponin.The present invention provides one kind and adopts water as solvent, with reference to ultrasound-assisted extraction technology and magnetic water treatment device, expeditiously to extract the method for the natural surfactant gleditschiasaponin in Chinese honey locust, this method is safe and environment-friendly, it is quick, inexpensive, suitable for commercial Application, and the gleditschiasaponin extract solution obtained may be directly applied in personal care product and household care product without purifying concentration.Wherein the power of ultrasonic wave is 300 500W, and the Ultrasonic Radiation time is 6 10 minutes, and Extracting temperature is 80 90 DEG C, and the weight ratio of Chinese honey locust and water is 1:2~1:4, extract 1~3 hour.

Description

A kind of extraction method of saponin

technical field

The invention relates to a method for extracting saponins, in particular to a method for extracting saponins using water as a solvent.

Background technique

Honey locust tree (Gleditsia Linn) is a leguminous plant, and there are about twelve species in the subfamily Hematoxylin. There are six common species: Gleditsia sinensis Lam, Gymnoclabrs chinensis, G. melanacantha, Gleditisia heterophylla, G. vestita chunex How , Japanese honey locust tree (Gleditsia. japonica). Among them, Gleditsia sinensis Lam (also known as Chinese honey locust) is a unique species in my country, and its growth and distribution area accounts for about half of my country's land area. Honey locust tree is a deciduous tree with a tall and straight trunk and lush leaves. Its growth life can reach 600 years and its fruit-producing period can reach 200 years.

The whole body of the honey locust tree can be used. The trunk has good wood, corrosion and wear resistance, and is an excellent industrial wood. Saponin thorn is rich in flavonoids, saponins, amino acids, phenols and other medicinal ingredients. It is a traditional Chinese medicinal material and a compatible Chinese herbal medicine for the treatment of breast cancer, lung cancer and other diseases. Pig tooth soap is a deformed small pod produced by the acacia tree when it is traumatized. It has the effects of eliminating phlegm and resuscitation, dispelling stagnation and reducing swelling. It is one of the traditional Chinese medicines collected in the Chinese Pharmacopoeia. Honey locust fruit includes honey locust seeds and honey locusts. Acacia seeds can extract acacia gum, which can be widely used in cosmetics, petroleum exploration, food and medicine, printing and dyeing textiles, mineral processing and mining and other industries as thickeners, stabilizers, adhesives, etc. Acacia saponin contains a large amount of natural surfactant saponin, which is one of the oldest surfactants and has been used by people to wash clothes, hair, fur, etc. in ancient times. In addition, acacia saponins also have the effects of killing insects, eliminating phlegm, resuscitation, and searching wind.

The root system of the acacia tree is developed, and it is drought-resistant and water-saving. It is an ideal ecological tree species. At present, countries all over the world and China are planting acacia trees in large quantities, as farmland, farm shelter forests, wild animal and plant protection forests, urban and rural landscape forests, and soil and water conservation forests. But a large amount of acacia resources are allowed to rot and are not well utilized, wasting this extremely valuable natural product resource in vain. Acacia saponins are rich in a large amount of natural surfactant saponins. If the saponins in acacia are extracted and successfully applied to personal care and home care products, it will greatly promote the planting of acacia trees and its downstream industrial chain in-depth development. Not only is it beneficial to afforestation, returning farmland to forests, and promoting the development of ecological and economic agriculture, but also the application of natural high-quality plant raw materials in cosmetics will definitely promote the upgrading of the cosmetics industry and benefit the general public. It can be seen that the industrial extraction and application research of saponins meet the needs of green and sustainable development in modern society.

The molecular structure of saponins is very complex. The molecular structure contains not only lipophilic group saponin, but also hydrophilic groups such as sugar and uronic acid. The molecular structure of saponins is shown in the following formula. Common saponins are pentacyclic triterpenoids. When R1 is H, it is an oleanolic acid structure, and when R1 is OH, it is a echinocytic acid structure. The sugars in saponins include glucose, xylose, arabinose, rhamnose, galactose, etc., and the uronic acids in saponins include galacturonic acid, glucuronic acid, etc.

Commonly used extraction methods for saponins include methanol or ethanol extraction, percolation, isopropanol dissolution and recrystallization, reflux extraction, supercritical fluid extraction, semi-bionic extraction, microwave-assisted extraction, and ultra-high pressure extraction. and many more. However, the above-mentioned extraction methods are often cumbersome to operate, use a large amount of solvent, and are expensive in equipment, and are not suitable for large-scale industrial operation.

The molecular weight of saponins is about 1600, and the molecules contain more polar groups such as hydroxyl, carboxyl, and ester groups. According to the principle of similar compatibility, saponins are easily soluble in polar solvents. At present, the most commonly used polar solvents in industry are methanol, ethanol, and water, so the above three solvents are the most suitable solvents for extracting saponins in industry.

Li Yingqiao (The extraction process of saponins and the determination of their surfactant properties [D]. Jilin Agricultural University master's degree thesis, 2011) used methanol as a solvent to study the extraction method of saponins in detail. The results showed that 75 % methanol was extracted for 1 hour and extracted three times in total, and the extraction rate of saponins was 22.39%. Apparently, the extraction rate of saponins in this extraction method is on the low side, and 77.61% of saponins have not been extracted yet. This extraction scheme fails to make full use of saponins resources. Furthermore, methanol is a highly toxic chemical. Using methanol as a solvent to extract saponins, the residual methanol in the product will definitely bring safety hazards to the use of saponins.

Zhang Chunxia et al. (The performance and application of natural surfactant saponins [J]. Journal of Zhengzhou Institute of Light Industry, 1993, 8 (2): 56-61) used ethanol as a solvent to extract saponins and studied their surface activity. Ethanol is a dangerous chemical, flammable and explosive, and a large amount of solvent consumption and cumbersome vacuum distillation procedures are bound to greatly hinder the industrial application of saponins. Acacia saponin extracted by ethanol will contain more fat-soluble components, which will seriously affect the foam and other surfactant properties of acacia saponin, and will also deepen the color of acacia saponin, which is not conducive to the use of acacia saponin in personal care and household Application in nursing products.

Liang Jingyi et al. (Research on the extraction process of saponins from saponins [J]. China Wild Plant Resources, 2003, 22(6):58-61) used water as solvent to conduct an orthogonal experimental optimization study on the extraction process of saponins from saponins. The experimental results showed that , using water as the solvent to extract saponins, the best process conditions are solid-to-liquid ratio 1:10, extraction temperature 45°C, extraction time 18 hours, extraction three times, the extraction rate of saponins is 90.9%. The extraction time of this process is 54 hours (one extraction time is 18 hours and extracts three times altogether), obviously the extraction time is too long. The place of origin of the saponins used in the paper is Beijing, and the content of saponins is 32%. According to the above extraction process, it can be inferred that the active matter content of saponins in the extract is about 1%. Such a low active matter content is obviously not suitable for direct extraction. Used in personal care and household care products industry.

To sum up, there are many methods for extracting saponins from saponins, but they are often complicated to operate, consume a lot of solvents, or are not suitable for large-scale industrial production at all. So it is particularly urgent to establish a safe, environmentally friendly, fast and low-cost method for extracting saponins from saponins.

Contents of the invention

The present invention uses the most abundant and cheap water as a solvent, combined with ultrasonic-assisted extraction technology and magnetized water treatment equipment, to efficiently extract the natural surfactant saponins in saponins. The method is safe, environmentally friendly, fast, low-cost, suitable for For industrial applications, the obtained saponin is a high-quality natural raw material, and the obtained saponin extract can be directly applied to personal care products and home care products without purification and concentration.

The object of the present invention is achieved through the following technical solutions.

The invention provides a method for extracting acacia saponin, which uses acacia as a raw material, water as a solvent, and simultaneously uses a magnetized water treatment device and ultrasonic-assisted extraction technology.

Both magnetized water treatment technology and ultrasonic-assisted extraction technology are new technologies and new technologies developed in recent decades. The present invention organically combines the above two technologies and applies them to the method for extracting saponins in saponins to improve The extraction rate of saponins and the shortening of extraction time provide the possibility for the industrial application of saponins.

The magnetized water treatment technology utilizes a magnetized water treatment device, please refer to accompanying drawing 1, which includes a magnetized water storage chamber (1), a motor (2), a reducer (3), a drive shaft (4), and a magnetized impeller (5). The motor (2) is connected to the reducer (3) and arranged on the top of the magnetized water storage chamber (1), and the transmission shaft (4) is connected to the reducer (3) and arranged on the magnetized water storage chamber (1) Inside, the magnetized impeller (5) is connected to the transmission shaft (4);

Preferably, a magnetization chamber (12) is arranged at the lower end of the magnetization water storage chamber (1), wherein a magnet (6) is arranged inside the magnetization chamber (12);

Preferably, a jacket (7) is provided outside the magnetized water storage chamber (1), a cold water outlet (8) is arranged at one end of the jacket (7), and a cold water inlet (9) is arranged at the other end;

Preferably, there are multiple magnets (6) inside the magnetization chamber (12);

Preferably, the magnet (6) is a natural magnet;

Preferably, the mass ratio of the natural magnet to the deionized water is 1:10-20;

Preferably, the natural magnet is Fe3O4;

Preferably, the magnetized water treatment device is 200L.

For ultrasonic-assisted extraction technology, the power of ultrasonic waves and the radiation time of ultrasonic waves have a great influence on the extraction efficiency. The power of the ultrasonic wave in the present invention is 300-500W, preferably 300W; the ultrasonic radiation time is 6-10 minutes, preferably 6 minutes.

Temperature has a great influence on the rate of chemical reactions and the equilibrium of chemical reactions. The lower extraction temperature will inevitably reduce the solubility of saponins in water, and the extraction rate will be slower. However, if the temperature is too high, it may cause partial hydrolysis of saponin, which reduces the extraction rate. Therefore, the extraction temperature of saponins is a very critical process parameter.

The solid-to-liquid ratio is the mass ratio of saponins to water. Obviously, with the increase of water, the extraction rate will increase accordingly, but the increase of water will lead to a decrease in the concentration of saponin active substances in the final extract. In the current personal care and home care industry standards, the minimum total surfactant content standards for shampoo, shower gel, hand sanitizer, laundry detergent, and dish soap are 10%, 7%, 7%, 15%, and 15% respectively . Then, the low content saponin natural surfactant extract must be concentrated before it can be used in the above products. The concentration process of the extract will inevitably bring about adverse factors such as energy consumption and increase in labor costs. Therefore, the choice of solid-liquid ratio in the extraction process is very critical.

The active ingredients of saponin from saponins need to pass through the cell membrane and cell wall of saponins to be dissolved into the aqueous solution. Obviously, the extraction rate will increase correspondingly with the extension of time. However, the extraction time is too long. On the one hand, because the saponins in the aqueous solution are dissolved and reach saturation, the extraction rate does not increase after a prolonged period of time. On the contrary, the partial hydrolysis of the saponins leads to a decrease in the extraction rate; on the other hand, the prolongation of the extraction time will lead to industrial energy consumption. , labor costs, etc. will increase exponentially, which is not conducive to the industrialization of the project. Therefore, the choice of extraction time is particularly important for the extraction of saponins from saponins.

In the present invention, it has been proved by experiments that the preferred extraction temperature is 80-90°C, more preferably 85°C; the weight ratio of acacia to water is 1:2 to 1:4, more preferably 1:3; extraction takes 1 to 3 hours , more preferably 3 hours.

Various saponins can be used in the extraction method of saponins of the present invention, preferably Chinese saponins (Gleditsiasinensis Lam).

The present invention further provides the saponin extract obtained according to the above-mentioned method of the present invention.

In another embodiment of the present invention, the present invention provides a personal care product comprising the saponins extract of the present invention, and the personal care product may be shampoo, shower gel, hand sanitizer, etc.

In another embodiment of the present invention, the present invention provides a household care product comprising the saponin extract of the present invention, and the personal care product may be laundry detergent, detergent, etc.

The invention obtains a high-content saponin natural surfactant through a simple water extraction method. The surfactant can be widely used in personal care products and household care products, as the main surfactant or auxiliary surfactant of shampoo, shower gel, hand sanitizer, laundry detergent, detergent and other products. Since the lipophilic groups of natural surfactants of saponins are triterpenoids, and the hydrophilic groups are polysaccharides and other components, from the perspective of chemical structure, the affinity of saponins to the skin must be far greater than that of synthetic surfactants. The irritation to the skin must be far less than that of synthetic surfactants. It can be seen that saponins natural surfactants can also be used in high-end cleaning products or baby cleaning products.

The present invention intends to use the most abundant and cheap water as a solvent, combined with ultrasonic-assisted extraction technology and magnetized water treatment equipment to extract the natural surfactant saponin in saponins. The industrial production conditions explored in the present invention are not only safe, environmentally friendly, and fast , low cost, and the extraction rate of saponins is very high. Such a simple and rapid operation process provides a practical operation plan for large-scale utilization of acacia resources.

In addition to containing saponin natural surfactant in the above-mentioned saponin extract, it also contains a small amount of chemical components such as cellulose, protein and polysaccharide. Cellulose, protein, polysaccharides, etc. are all chemical components allowed in personal care and home care products, and the above components have functions such as thickening and moisturizing. Impurities such as cellulose, protein, and polysaccharides coexisting in the extract do not need to be separated and purified. The extraction solvent is water, and it does not need to be concentrated by vacuum distillation. Therefore, the saponin extract obtained by the above method can be directly used in personal care and household care products as the main surfactant or auxiliary surfactant without purification and separation, which greatly reduces the production cost.

Description of drawings

Below, describe embodiment of the present invention in detail in conjunction with accompanying drawing, wherein:

Figure 1 is a magnetized water treatment device.

The infrared spectrogram of Fig. 2 saponins standard sample.

Detailed ways

The present invention will be further described in detail below in conjunction with specific embodiments, and the given examples are only for clarifying the present invention, not for limiting the scope of the present invention.

The test methods in the following examples, unless otherwise specified, are conventional methods, and the raw materials, reagent materials, etc. used in the following examples, unless otherwise specified, are commercially available products.

Embodiment 1: preparation, content determination and qualitative inspection of saponins standard substance

The acacia used in this experiment came from Gleditsia sinensis Lam in Henan, and other chemical reagents were of analytical grade, from Sinopharm Chemical Reagent Company.

(1) Preparation of saponin standard substance and determination of saponin content (weighing method):

Weigh 2 g (accurate to 0.0001 g) of honey locust dried at 105°C to a constant weight, and use ether as solvent for Soxhlet extraction for 6 hours to remove the lipid components in the honey locust. Use methanol as solvent for Soxhlet extraction for 6 hours, add an equal volume of diethyl ether to the methanol extract to collect the precipitated precipitate, and repeat the operation until the precipitate is no longer precipitated after adding diethyl ether. Dissolve the precipitated precipitate in water, then extract the saponin with n-butanol, repeat the operation until the n-butanol phase has no saponins, combine the n-butanol extract, evaporate the n-butanol, and put the residue into a weighing bottle In the process, dry at 105°C to constant weight to obtain the standard saponins of saponins, and weigh them. This method can measure the content of saponins in saponins. Calculated as follows:

X—the percentage content of saponins of saponins, %;

W1—mass of acacia, g;

W2—the mass of the weighing bottle dried to the constant weight, g;

W3—the mass of the sample and weighing bottle dried to constant weight, g.

It has been determined that the content of saponins in the saponins produced in Henan is 29.8%.

(2) Qualitative inspection of saponin standard substance (acetic anhydride-concentrated sulfuric acid color method):

Experimental principle: Saponins of saponins first turn yellow when they meet concentrated sulfuric acid, then slowly turn red, then turn purple, and finally turn blue.

Experimental method: Dissolve a small amount of saponin standard sample obtained above in a test tube filled with acetic anhydride, then add concentrated sulfuric acid-acetic anhydride reagent dropwise to the test tube, and observe the color change in the test tube.

Experimental results: the color change order of saponin standard sample in the test tube is yellow-red-purple-blue.

(3) melting point and infrared spectroscopic analysis of saponins standard substance:

Carry out melting point, infrared spectrum, thin-layer chromatography and liquid chromatography analysis to the saponins standard substance sample obtained above.

Melting point: The measured melting point range of saponins is 191-195°C, and the literature value is 192-193°C, which is basically consistent.

Infrared spectroscopic analysis: KBr tablet method. The infrared spectrogram is shown in Figure 2.

Thin-layer chromatography: silica gel G was used as the adsorbent, ethyl acetate-acetic acid-water (8:2:1) was used as the developer, and 50% sulfuric acid was used as the developer.

Liquid chromatography: ODS-2 C ₁₈ reverse-phase column (4.6mm×250mm, 5μm), column temperature: 40°C; mobile phase: V (acetonitrile): V (water) = 4:1, 0.8 mL·min ^-1 ; Ultraviolet detector, detection wavelength 454nm.

After verification, it is confirmed that the extracted ingredients are saponins.

Embodiment 2: Extraction condition screening of saponins extract

In this example, referring to the method in Example 1, the content of saponin in the saponin extract was measured, and the extraction rate of saponin was calculated accordingly.

Embodiment 2-1: the selection of saponin ultrasonic power

In the 200L magnetized water treatment device (please refer to Fig. 1), the fixed acacia: the solid-liquid ratio of water is 1:3 (50 kilograms of acacia: 150 kilograms of water), the total ultrasonic radiation time of setting is 6 minutes, and the extraction time is 2 Hours, the extraction temperature was 85°C. After the extraction, the saponin extract was obtained by filtration. The influence of ultrasonic power on the extraction rate of saponin from saponins is shown in Table 1 below.

Table 1 Effect of ultrasonic power on extraction rate

It can be seen from the above table that a good extraction rate is obtained in the range of 300W-500W. When the ultrasonic power is at 300W, the extraction rate of saponins is already high. Continue to increase the ultrasonic power, and the extraction rate of saponins hardly changes.

Example 2-2: Selection of Ultrasonic Radiation Time

In the 200L magnetized water treatment device (please refer to Fig. 1), fixed acacia: the solid-liquid ratio of water is 1:3 (50 kilograms of acacia: 150 kilograms of water), the power of setting ultrasonic wave is 300W, and extraction time is 2 hours, The extraction temperature was 85°C. After the extraction, the saponin extract was obtained by filtration.

The impact of the radiation time of ultrasonic waves on the extraction rate of saponin from saponins is shown in Table 2 below.

Table 2 Effect of ultrasonic radiation time on extraction rate

As can be seen from the above table, a better extraction rate has been obtained in the range of 6-10 minutes. When the radiation time is 6 minutes, the extraction rate of saponin is already high. Continue to increase the radiation time, and the extraction rate of saponin hardly changes.

Embodiment 2-3: the selection of extraction temperature

In the 200L magnetized water treatment device (please refer to Figure 1), the solid-liquid ratio of fixed acacia: water is 1:3 (50 kg of acacia: 150 kg of water), the power of the ultrasonic wave is set to 300W, and the ultrasonic radiation time is 6 minutes , the extraction time is 2 hours. After the extraction, the saponin extract was obtained by filtration. The influence of extraction temperature on the extraction rate of saponin from saponins is shown in Table 3 below.

Table 3 Effect of extraction temperature on extraction rate

It can be seen from the above table that when the extraction temperature is 80-90°C, the extraction rate of saponins is higher. When the temperature was below 85°C, the extraction rate increased gradually with the increase of temperature, and when the temperature was above 85°C, the extraction rate decreased with the increase of temperature. It may be caused by partial hydrolysis of saponin when the temperature is higher than 85°C.

Embodiment 2-4: selection of extraction time

In the 200L magnetized water treatment device (please refer to Figure 1), the solid-liquid ratio of fixed acacia: water is 1:3 (50 kg of acacia: 150 kg of water), the power of the ultrasonic wave is set to 300W, and the ultrasonic radiation time is 6 minutes , the extraction temperature was 85°C. After the extraction, the saponin extract was obtained by filtration. The impact of extraction time on saponins is shown in Table 4 below.

Table 4 Effect of extraction time on extraction rate

It can be seen from the above table that when the extraction time is 2 to 4 hours, the extraction rate of saponins is relatively high. When the extraction time was 2 hours, the extraction rate of saponins reached the highest value.

Embodiment 2-5: the selection of solid-liquid ratio

In the 200L magnetized water treatment device (please refer to FIG. 1 ), the power of the ultrasonic wave is set to 300W, the ultrasonic radiation time is 6 minutes, the extraction temperature is 85° C., and the extraction time is 2 hours. After the extraction, the saponin extract was obtained by filtration. The influence of solid-liquid ratio on the extraction rate of saponin from saponins is shown in Table 5 below.

Table 5 Effect of solid-liquid ratio on extraction rate

solid to liquid ratio 1:1 1:2 1:3 1:4 1:5 Extraction rate/% 65.88 77.65 85.35 85.42 85.61

It can be seen from the above table that the extraction rate of saponins increases with the increase of water. When the solid-liquid ratio is 1:3-1:5, the extraction rate of saponins is higher. When the ratio of solid-liquid reaches 1:3 , the extraction rate of acacia saponin did not change significantly with the increase of water ratio.

Embodiment 2-6: Orthogonal Experimental Optimization of Extraction Conditions

In the 200L magnetized water treatment device (see FIG. 1 ), the power of the ultrasonic wave is set to 300W, and the ultrasonic radiation time is 6 minutes.

Orthogonal experiments were used to further optimize the extraction process conditions of saponins. Selecting extraction temperature (T), extraction time (t), solid-liquid ratio (n) is the factor of orthogonal test, evaluation standard is the extraction rate of saponins, factor level table is shown in the following table 6, and the results of orthogonal test are shown in the following table 7.

Table 6 Factor level table

T(extraction temperature)/℃ t(extraction time)/h n (solid-liquid ratio) 1 80 1 1:2 2 85 2 1:3 3 90 3 1:4

Table 7 Orthogonal test results

In the above table, I is the sum of the first level of saponin extraction rate corresponding to each factor, II is the sum of the extraction rate of saponin of the second level corresponding to each factor, and III is the third level of each factor The sum of the saponins extraction rate of each level, I/3 is the average value of the first level saponin extraction rate corresponding to each factor, and II/3 is the average value of the second level saponin extraction rate corresponding to each factor , III/3 is the average value of the saponins extraction rate of the third level corresponding to each factor.

It can be seen from Table 7 that the saponin yield corresponding to experiment number 9 is the highest, and the yield of saponin corresponding to experiment number 5 is the second. The conditions of the two experiments are shown in Table 8 below.

Table 8 Orthogonal test visual analysis table

From the range analysis, it can be seen that the factors that have the greatest impact on the extraction rate of saponins are: extraction time (t) > solid-liquid ratio (n) > extraction temperature (T). The optimal extraction process conditions for saponins are T ₂ t ₃ n ₂ , that is, the extraction temperature is 85°C, the extraction time is 3 hours, and the solid-liquid ratio is 1:3.

Three experiments were carried out to verify the optimal process conditions for the extraction of saponins from saponins. The results are shown in Table 9 below. The average extraction rate of saponins from saponins is 86.56%.

Table 9 Confirmatory test

Embodiment 3: the analysis of physical and chemical index of saponin saponin extract

The physical and chemical indicators of the saponins extract mixture obtained from Experiments 10-12 in Example 7 above are shown in Table 10 below.

Table 10 Physicochemical indicators of saponins extract*

*The test method refers to "Safety and Technical Specifications for Cosmetics" (2015 edition)

From the test data in Table 10, it can be seen that the physical and chemical indicators of the above-mentioned saponin extract of saponins all meet the requirements for raw materials in the "Safety and Technical Specifications for Cosmetics" (2015 edition). It can be seen that the saponin extract can be directly added to the cosmetic formula as a cosmetic raw material.

Embodiment 4: the application of saponin saponin extract of the present invention

Embodiment 4-1: the preparation of soap locust shampoo

Take by weighing the following raw materials: saponin saponin extract of the present invention 4000g, disodium EDTA 5g, cocamidopropyl betaine 300g, cocamide DEA 100g, emulsified silicone oil 150g, guar gum hydroxypropyl trimethyl ammonium chloride 10g, polyquaternium-10 5g, citric acid 0.5g, sodium chloride 75g, Cathone 4g, essence 17.5g, deionized water 333g.

Guar gum hydroxypropyltrimethylammonium chloride and 200g of water were stirred and mixed to obtain an aqueous solution of guar gum hydroxypropyltrimethylammonium chloride. Stir and mix polyquaternium-10 and 133 g of water to obtain polyquaternium-10 aqueous solution.

Add the saponins extract of the present invention into a stirring pot and heat to 75°C. Add guar gum hydroxypropyltrimethylammonium chloride aqueous solution, polyquaternium-10 aqueous solution, stir well; add disodium EDTA, cocamidopropyl betaine, cocamide DEA, stir well; cool down to 45 ℃, add emulsified silicone oil, citric acid, sodium chloride, Cathone, and essence, stir for 30 minutes, and then filter with a 200-mesh filter to obtain the product.

Embodiment 4-2: the preparation of soap locust shower gel

Take by weighing the following raw materials: saponin extract of saponins of the present invention 2250g, disodium EDTA 5g, cocamidopropyl betaine 300g, cocamide DEA 100g, citric acid 0.5g, sodium chloride 100g, Kathon 4g, essence 17.5g g, deionized water 2223g.

Add deionized water, saponin extract of saponins of the present invention, disodium EDTA, cocamidopropyl betaine, cocamide DEA, citric acid, sodium chloride, cassonne, and essence into the stirring pot in turn, and stir for 60 minutes , and then filter with a 200-mesh filter, that is.

Embodiment 4-3: the preparation of soap locust hand sanitizer

Take by weighing following each raw material: saponin saponin extract 1250g of the present invention, sodium laureth sulfate 125g, disodium EDTA 5g, cocamidopropyl betaine 300g, cocamide DEA100g, citric acid 0.5g, sodium chloride 100g, Kason 4g, essence 17.5g, deionized water 3098g.

Add deionized water, saponin extract of saponins of the present invention, sodium lauryl ether sulfate, disodium EDTA, cocamidopropyl betaine, cocamide DEA, citric acid, sodium chloride, Cathone, and essence in sequence Put it in a stirring pot, stir for 60 minutes, and then filter it with a 200-mesh filter.

Embodiment 4-4: the preparation of soap locust laundry detergent

Take by weighing the following raw materials: saponin extract of saponins of the present invention 500g, sodium dodecylbenzenesulfonate 300g, laureth-9 200g, sodium laureth sulfate 300g, sodium citrate 25g, hydroxyethyl cellulose Su 35g, DMDM hydantoin 10g, essence 7.5g, deionized water 3622.5g.

Deionized water, saponin extract of the present invention, sodium dodecylbenzenesulfonate, lauryl ether-9, sodium lauryl ether sulfate, sodium citrate, hydroxyethyl cellulose, DMDM benzine Add the ureide and essence into the stirring pot, stir for 60 minutes, and then filter with a 200-mesh filter to obtain the product.

Embodiment 4-5: the preparation of soap locust detergent

Take by weighing the following raw materials: 750g of saponin extract of saponins of the present invention, 300g of sodium dodecylbenzenesulfonate, 75g of laureth-9, 500g of sodium laureth sulfate, 25g of sodium citrate, and 75g of sodium chloride , DMDM hydantoin 10g, essence 7.5g, deionized water 3257.5g.

Deionized water, saponin extract of saponins of the present invention, sodium dodecylbenzenesulfonate, laureth-9, sodium laureth sulfate, sodium citrate, sodium chloride, DMDM hydantoin 1. Add the essence into the stirring pot, stir for 60 minutes, and then filter with a 200-mesh filter to get the product.

Claims (10)

1. a kind of extracting method of gleditschiasaponin, it is characterised in that using Chinese honey locust as raw material, water uses magnetic as solvent Water chemical treatment device and ultrasound-assisted extraction technology.

2. the extracting method of gleditschiasaponin according to claim 1, wherein the magnetic water treatment device used includes magnetization Reservoir chamber (1), motor (2), reductor (3), power transmission shaft (4), magnetization impeller (5), the motor (2) connect the reductor (3) and described magnetization reservoir chamber (1) top is arranged on, power transmission shaft (4) the connection reductor (3) is simultaneously arranged at magnetization reservoir chamber (1) it is internal, magnetization impeller (5) the connection power transmission shaft (4)；

Preferably, described magnetization reservoir chamber (1) lower end sets magnetization chamber (12), wherein, magnet is set inside magnetization chamber (12) (6)；

Preferably, chuck (7) is set on the outside of the magnetization reservoir chamber (1), and chuck (7) one end sets cold water delivery port (8), another End sets cold water inlet (9)；

Preferably, the internal magnet (6) of the magnetization chamber (12) is multiple；

Preferably, the magnet (6) is native magnet；

Preferably, the native magnet and the mass ratio of the deionized water are 1:10-20；

Preferably, the native magnet is Fe3O4；

Preferably, the magnetic water treatment device is 200L.

3. the power of the extracting method of gleditschiasaponin according to claim 1 or 2, wherein ultrasonic wave is 300-500W, surpass The acoustic irradiation time is 6-10 minutes.

4. the power of the extracting method of gleditschiasaponin according to claim 3, wherein ultrasonic wave is 300W, Ultrasonic Radiation Time is 6 minutes.

5. the extracting method of the gleditschiasaponin according to claim any one of 1-4, wherein Extracting temperature are 80-90 DEG C, soap The weight of pod and water ratio is 1:2~1:4, extract 1~3 hour.

6. the extracting method of gleditschiasaponin according to claim 5, wherein Extracting temperature are 85 DEG C, the weight of Chinese honey locust and water Than for 1:3, extract 3 hours.

7. the extracting method of the gleditschiasaponin according to claim any one of 1-6, the Chinese honey locust used is Chinese Chinese honey locust (Gleditsia sinensis Lam)。

8. the gleditschiasaponin extract solution obtained according to any one of claim 1-7 extracting method.

9. a kind of personal-care supplies, the gleditschiasaponin extract solution comprising claim 8, it is preferably shampoo, shower cream, washed Hand liquid.

10. a kind of household care products, the gleditschiasaponin extract solution comprising claim 8, it is preferably liquid detergent, liquid detergent.