CN107801568A - A kind of quick propagation method of Termitomyces albuminosus with black skin parent species - Google Patents
A kind of quick propagation method of Termitomyces albuminosus with black skin parent species Download PDFInfo
- Publication number
- CN107801568A CN107801568A CN201711229362.8A CN201711229362A CN107801568A CN 107801568 A CN107801568 A CN 107801568A CN 201711229362 A CN201711229362 A CN 201711229362A CN 107801568 A CN107801568 A CN 107801568A
- Authority
- CN
- China
- Prior art keywords
- test tube
- parent species
- expansion
- termitomyces albuminosus
- potato dextrose
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Botany (AREA)
- Mycology (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
A kind of quick propagation method of Termitomyces albuminosus with black skin parent species, comprises the following steps：Step 1）：Prepare sterilized water and test tube slant；Step 2）：Hypha suspension of the mother seeds is prepared, the high-quality healthy and strong parent species being separately cultured from tissue, aseptically, injects sterilized water in by expansion parent species test tube, mixing broken growth with transfer needle has by the culture medium of expansion parent species, and hypha suspension of the mother seeds is made；Step 3）：Expansion connects, and aseptically, hypha suspension of the mother seeds 5mL is extracted, in each step 1）The expansion for filling improvement potato dextrose agar prepared connects hypha suspension of the mother seeds in test tube and balanced；Step 4）：Culture, expansion is connect into test tube be put into and incubated in constant incubator cover with test tube slant culture medium to mycelia.Operating method of the present invention is simple, and inoculation speed is fast, and 5mL mycelia suspension, which can expand, connects 100 parent species, the min of used time 10 15, and bacterium germination time when casting skin Termitomyces albuminosus parent species expand numerous can be greatly shortened.
Description
Technical field
The invention belongs to Termitomyces albuminosus culture technique field, and in particular to a kind of fast-propagation side of Termitomyces albuminosus with black skin parent species
Method.
Background technology
Casting skin Termitomyces albuminosus scientific name cigarette ash Termitomyces albuminosus, belong to one kind of Termitomyces albuminosus, because Termitomyces albuminosus fine and tender taste, smell are dense
Fragrant, delicious flavour and with invigorating qi and benefiting blood, beneficial stomach and other effects, therefore wild casting skin Termitomyces albuminosus have been difficult to meet the market demand, people
Start artificial large-scale culture.But the large-scale production of casting skin Termitomyces albuminosus be unable to do without the expanding propagation of parent species.Parent species are that chicken is indulged
The basis of bacterium production, the parent species of high-quality rejuvenation are the prerequisites of Termitomyces albuminosus high-yield culturing.And generally use passes in the prior art
The one-point type inoculation method of system, the bacterium germination time is partially long, both influences to produce, and because cell age is long, mycelia is weak, bacterium when causing to expand numerous again
Kind activity decrease, therefore the artificial culture for casting skin Termitomyces albuminosus has to improve the inoculation method of parent species.
The content of the invention
The problem of existing for prior art, it is an object of the invention to provide a kind of operating method is simple, inoculation speed
Hurry up, the bacterium germination time is short, improve mycelia quality Termitomyces albuminosus with black skin parent species quick propagation method, to solve casting skin in the prior art
Parent species inoculation speed is slow in the artificial incubation of collybia albuminosa, mycelial growth is slow, inferior problem.
In order to realize the above object the technical solution adopted by the present invention is：A kind of fast-propagation of Termitomyces albuminosus with black skin parent species
Method, comprise the following steps：
Step 1)：Prepare sterilized water and test tube slant；
Step 1.1)：Water, bottleneck brown paper tying are added in triangular flask；
Step 1.2)：Culture medium using improvement potato dextrose agar, its component include potato 150g, can
Dissolubility alcohol grain 100-150g, glucose 20g, potassium dihydrogen phosphate 2g, magnesium sulfate 0.5g, agar 20g, yeast extract 2-3g,
VB15-10mg, suitable quantity of water；
Step 1.3)：The triangular flask for filling water and the test tube for filling improvement potato dextrose agar are gone out
Bacterium, improvement potato dextrose agar is put into inclined-plane of the top away from test tube mouth 30-50mm in 5min after sterilizing, is put into
Slant medium length 130mm, will sterilized water and improvement potato dextrose agar cooling after it is standby；
Step 2)：Prepare hypha suspension of the mother seeds；
The high-quality healthy and strong parent species being separately cultured from tissue, aseptically, are existed with Sterile pipette or syringe
Sterilized water is injected in expansion parent species test tube, mixing broken growth with transfer needle has by the culture medium of expansion parent species, and hypha suspension of the mother seeds is made；
Step 3)：Expansion connects；
Aseptically, hypha suspension of the mother seeds 5mL is extracted with Sterile pipette or syringe, is made in each step 1)
The standby expansion for filling improvement potato dextrose agar connects injection 0.05mL hypha suspension of the mother seeds in test tube and balanced；
Step 4)：Culture
Expansion is connect into test tube and is put into incubated in constant incubator, cultivation temperature is stable at 22-28 DEG C, cultivates 6-8 days bacterium
Silk can cover with 130mm test tube slant culture medium.
Further, the preparation method of improvement potato dextrose agar is in the step 1.2)：First by horse
Bell potato cleans peeling, then weighs 150g choppings, after adding water 1000mL to be boiled by slow fire 20-30min, with four layers of filtered through gauze, then adds
Enter soluble alcohol grain 100-150g, glucose 20g, potassium dihydrogen phosphate 2g, magnesium sulfate 0.5g, agar 20g, yeast extract 2-3g
And VB15-10mg, continue heating stirring and mix to agar thawing, then supply water to 1000mL, when temperature is not less than 70 DEG C
Culture medium is dispensed to the 1/4-1/5 of test tube length, test tube mouth and adds cotton carding plug, is then tied with brown paper outside test tube mouth tampon
Prick.
Further, sterilization process is in the step 1.3)：By the triangular flask for filling water and fill improvement potato Portugal
The test tube of grape sugar agar medium loads pressure cooker, autoclaving 30min under 0.11-0.12MPa.
Further, it is from the Termitomyces albuminosus with black skin battalion grown that the tissue of parent species, which is separately cultured process, in the step 2)
Support and tender body cell induction callus is extracted on body, be gradually trained mycelium, and choose pure white, healthy and strong mycelium and make
For parent species.
The present invention extracts tender body cell from the Termitomyces albuminosus with black skin trophosome grown and induces callus, gradually
Mycelium is trained, and chooses pure white, healthy and strong mycelium as parent species, enhances the mycelial quality of casting skin Termitomyces albuminosus.This
The culture medium that invention is selected adds DDS (soluble alcohol grain) in normal potato glucose agar medium, due in DDS
Rich in crude protein, crude fibre, available phosphorus, potassium, sodium and abundant amino acid, it can effectively accelerate the speed of growth of mycelia, and
DDS is generally acidity, is particularly conducive to the growth of collybia albuminosa mycelia.Culture medium is through autoclaving and whole nothing in incubation
Bacterium operates, and the mycelium of culture is pollution-free；In expansion connects incubation, inoculation speed is fast, and 5mL mycelia suspension, which can expand, connects 100
Parent species, used time 10-15min, bacterium germination time when casting skin Termitomyces albuminosus parent species expand numerous can be greatly shortened.
Compared with prior art, the invention has the advantages that：
Equipment is simple needed for the present invention, and operating method is simple, and cost is cheap, and inoculation speed is fast, and 5mL mycelia suspension, which can expand, to be connect
Bacterium germination time when casting skin Termitomyces albuminosus parent species expand numerous can be greatly shortened in 100 parent species, used time 10-15min, and cell age is short and one
Cause, and pass through the improvement to culture medium so that the Mycelial activity of culture is vigorous, is provided well for the production of casting skin Termitomyces albuminosus
Basis, parent species fast-propagation can be made, meet casting skin Termitomyces albuminosus large-scale production needed for high-quality, rejuvenation parent species.
Embodiment
The quick propagation method of the Termitomyces albuminosus with black skin parent species of the present invention is explained with reference to specific examples below,
It is but not as a limitation of the invention.
Embodiment 1
A kind of quick propagation method of Termitomyces albuminosus with black skin parent species, comprises the following steps：
Step 1)：Prepare sterilized water and test tube slant；
Step 1.1)：Water, bottleneck brown paper tying are added in triangular flask；
Step 1.2)：Culture medium using improvement potato dextrose agar, its component include potato 150g, can
Dissolubility alcohol grain 120g, glucose 20g, potassium dihydrogen phosphate 2g, magnesium sulfate 0.5g, agar 20g, yeast extract 3g, VB1 8mg、
Suitable quantity of water；Improvement potato dextrose agar preparation method be：Potato is first cleaned into peeling, then weighs 150g and cuts
It is broken, after adding water 1000mL to be boiled by slow fire 25min, with four layers of filtered through gauze, add soluble alcohol grain 120g, glucose 20g,
Potassium dihydrogen phosphate 2g, magnesium sulfate 0.5g, agar 20g, yeast extract 3g and VB18mg, continuation heating stirring, which is mixed to agar, melts
Change, then supply water to 1000mL, be well mixed, dispense culture medium to the 1/4 of test tube length when temperature is not less than 70 DEG C,
Test tube mouth adds cotton carding plug, elastic appropriateness, is not fallen off with lifting tampon test tube, extracts tampon and have slight sound to be advisable, then 7 examinations
Manage as 1 group, tied up with brown paper outside test tube mouth tampon；
Step 1.3)：The triangular flask for filling water and the test tube for filling improvement potato dextrose agar are gone out
Bacterium, the triangular flask for filling water and the test tube for filling improvement potato dextrose agar are loaded into pressure cooker, under 0.11MPa
Autoclaving 30min；Improvement potato dextrose agar is put into top away from test tube mouth 30mm's in 5min after sterilizing
Inclined-plane, the slant medium length 130mm being put into, by sterilized water and improvement potato dextrose agar cooling standby
With；
Step 2)：Prepare hypha suspension of the mother seeds；
Tender body cell is extracted from the Termitomyces albuminosus with black skin trophosome grown and induces callus, is gradually trained
Mycelium, and choose pure white, healthy and strong mycelium and be more than 70% as parent species, cultivation biology efficiency, aseptically, use
Sterile pipette or syringe inject sterilized water in by expansion parent species test tube, and mixing broken growth with transfer needle has by the culture of expansion parent species
Base, hypha suspension of the mother seeds is made；
Step 3)：Expansion connects；
Aseptically, hypha suspension of the mother seeds 5mL is extracted with Sterile pipette, is filled prepared by each step 1)
The expansion of improvement potato dextrose agar connects the drop of injection 2 (about 0.05mL) hypha suspension of the mother seeds in test tube and balanced, 5ml bacterium
Silk suspension, which can expand, connects 102 test tube stocks；
Step 4)：Culture；
Expansion is connect into test tube and is put into incubated in constant incubator, cultivation temperature is stable at 26 DEG C, and 6 days mycelia of culture are covered with
130mm test tube slant culture medium.
Embodiment 2
A kind of quick propagation method of Termitomyces albuminosus with black skin parent species, comprises the following steps：
Step 1)：Prepare sterilized water and test tube slant；
Step 1.1)：Water, bottleneck brown paper tying are added in triangular flask；
Step 1.2)：Culture medium using improvement potato dextrose agar, its component include potato 150g, can
Dissolubility alcohol grain 150g, glucose 20g, potassium dihydrogen phosphate 2g, magnesium sulfate 0.5g, agar 20g, yeast extract 2g, VB1 10mg、
Suitable quantity of water；Improvement potato dextrose agar preparation method be：Potato is first cleaned into peeling, then weighs 150g and cuts
It is broken, after adding water 1000mL to be boiled by slow fire 25min, with four layers of filtered through gauze, add soluble alcohol grain 150g, glucose 20g,
Potassium dihydrogen phosphate 2g, magnesium sulfate 0.5g, agar 20g, yeast extract 2g and VB110mg, continuation heating stirring, which is mixed to agar, melts
Change, then supply water to 1000mL, be well mixed, dispense culture medium to the 1/4 of test tube length when temperature is not less than 70 DEG C,
Test tube mouth adds cotton carding plug, elastic appropriateness, is not fallen off with lifting tampon test tube, extracts tampon and have slight sound to be advisable, then 7 examinations
Manage as 1 group, tied up with brown paper outside test tube mouth tampon；
Step 1.3)：The triangular flask for filling water and the test tube for filling improvement potato dextrose agar are gone out
Bacterium, the triangular flask for filling water and the test tube for filling improvement potato dextrose agar are loaded into pressure cooker, under 0.12MPa
Autoclaving 30min；Improvement potato dextrose agar is put into top away from test tube mouth 40mm's in 5min after sterilizing
Inclined-plane, the slant medium length 130mm being put into, by sterilized water and improvement potato dextrose agar cooling standby
With；
Step 2):Prepare hypha suspension of the mother seeds；
Tender body cell is extracted from the Termitomyces albuminosus with black skin trophosome grown and induces callus, is gradually trained
Mycelium, and choose pure white, healthy and strong mycelium and be more than 70% as parent species, cultivation biology efficiency, aseptically, use
Sterile pipette or syringe inject sterilized water in by expansion parent species test tube, and mixing broken growth with transfer needle has by the culture of expansion parent species
Base, hypha suspension of the mother seeds is made；
Step 3)：Expansion connects；
Aseptically, hypha suspension of the mother seeds 5mL is extracted with Sterile pipette, is filled prepared by each step 1)
The expansion of improvement potato dextrose agar connects the drop of injection 2 (about 0.05mL) hypha suspension of the mother seeds in test tube and balanced, 5ml bacterium
Silk suspension, which can expand, connects 105 test tube stocks；
Step 4)：Culture；
Expansion is connect into test tube and is put into incubated in constant incubator, cultivation temperature is stable at 26 DEG C, and 7 days mycelia of culture are covered with
130mm test tube slant culture medium.
Embodiment 3
A kind of quick propagation method of Termitomyces albuminosus with black skin parent species, comprises the following steps：
Step 1)：Prepare sterilized water and test tube slant；
Step 1.1)：Water, bottleneck brown paper tying are added in triangular flask；
Step 1.2)：Culture medium using improvement potato dextrose agar, its component include potato 150g, can
Dissolubility alcohol grain 100g, glucose 20g, potassium dihydrogen phosphate 2g, magnesium sulfate 0.5g, agar 20g, yeast extract 2.5g, VB1
8mg, suitable quantity of water；Improvement potato dextrose agar preparation method be：Potato is first cleaned into peeling, then weighed
150g is shredded, and after adding water 1000mL to be boiled by slow fire 25min, with four layers of filtered through gauze, adds soluble alcohol grain 100g, grape
Sugared 20g, potassium dihydrogen phosphate 2g, magnesium sulfate 0.5g, agar 20g, yeast extract 2.5g and VB18mg, continue heating stirring and mix
Melt to agar, then supply water to 1000mL, be well mixed, culture medium is dispensed to test tube when temperature is not less than 70 DEG C and grown
The 1/5 of degree, test tube mouth add cotton carding plug, elastic appropriateness, are not fallen off with lifting tampon test tube, extract tampon and have slight sound to be advisable,
Then 7 test tubes are 1 group, are tied up with brown paper outside test tube mouth tampon；
Step 1.3)：The triangular flask for filling water and the test tube for filling improvement potato dextrose agar are gone out
Bacterium, the triangular flask for filling water and the test tube for filling improvement potato dextrose agar are loaded into pressure cooker, under 0.11MPa
Autoclaving 30min；Improvement potato dextrose agar is put into top away from test tube mouth 30mm's in 5min after sterilizing
Inclined-plane, the slant medium length 130mm being put into, by sterilized water and improvement potato dextrose agar cooling standby
With；
Step 2):Prepare hypha suspension of the mother seeds；
Tender body cell is extracted from the Termitomyces albuminosus with black skin trophosome grown and induces callus, is gradually trained
Mycelium, and choose pure white, healthy and strong mycelium and be more than 70% as parent species, cultivation biology efficiency, aseptically, use
Sterile pipette or syringe inject sterilized water in by expansion parent species test tube, and mixing broken growth with transfer needle has by the culture of expansion parent species
Base, hypha suspension of the mother seeds is made；
Step 3)：Expansion connects；
Aseptically, hypha suspension of the mother seeds 5mL is extracted with Sterile pipette, is filled prepared by each step 1)
The expansion of improvement potato dextrose agar connects the drop of injection 2 (about 0.05mL) hypha suspension of the mother seeds in test tube and balanced, 5ml bacterium
Silk suspension, which can expand, connects 105 test tube stocks；
Step 4)：Culture；
Expansion is connect into test tube and is put into incubated in constant incubator, cultivation temperature is stable at 26 DEG C, and 6 days mycelia of culture are covered with
130mm test tube slant culture medium.
Contrast groups 1：Above carried from the Termitomyces albuminosus with black skin trophosome grown according to cultural method conventional in the prior art
Take tender body cell to induce callus, be gradually trained mycelium, and choose pure white, healthy and strong mycelium as parent species,
Parent species are transferred in the test tube equipped with PDA culture medium at 15-18 DEG C culture obtain mycelia and grow to will both have been obtained into during full inclined-plane
Ripe parent species, 12 days used times；Wherein contrast groups 1 are identical with inoculation test tube specification used in embodiment 1-3.
Embodiment 1-3 and contrast groups 1 the parent species growing state cultivated are tested, test result is as shown in table 1 below.
Table 1
Note:" ++++" represent that mycelia is dense, " +++ " represents that mycelia is closeer, and " ++ " represents that mycelia is sparse, and "+" represents mycelia
It is extremely dilute.
From upper table 1, casting skin Termitomyces albuminosus parent species reproductive speed is obviously higher than contrast made from 1-3 of the embodiment of the present invention
Group 1, and mycelial density, regularity and sturdy degree are superior to contrast groups 1, it was demonstrated that pass through the expansion of the casting skin Termitomyces albuminosus parent species of the present invention
The parent species speed of growth that breeding method obtains is fast, quality is high, solves that casting skin Termitomyces albuminosus parent species reproductive speed in the prior art is slow, production
Measure low, inferior problem.
Finally it should be noted that：The preferred embodiments of the present invention are the foregoing is only, are not intended to limit the invention,
Although the present invention is described in detail with reference to the foregoing embodiments, for those skilled in the art, it still may be used
To be modified to the technical scheme described in foregoing embodiments, or equivalent substitution is carried out to which part technical characteristic.
Within the spirit and principles of the invention, any modification, equivalent substitution and improvements made etc., it should be included in the present invention's
Within protection domain.
Claims (4)
1. a kind of quick propagation method of Termitomyces albuminosus with black skin parent species, it is characterised in that comprise the following steps：
Step 1）：Prepare sterilized water and test tube slant；
Step 1.1）：Water, bottleneck brown paper tying are added in triangular flask；
Step 1.2）：Culture medium includes potato 150g, solubility using improvement potato dextrose agar, its component
Alcohol grain 100-150g, glucose 20g, potassium dihydrogen phosphate 2g, magnesium sulfate 0.5g, agar 20g, yeast extract 2-3g, VB1 5-
10mg, suitable quantity of water；
Step 1.3）：The triangular flask for filling water and the test tube for filling improvement potato dextrose agar are sterilized,
Improvement potato dextrose agar is put into inclined-plane of the top away from test tube mouth 30-50mm in 5min after sterilizing, is put into
Slant medium length 130mm, will be standby after sterilized water and improvement potato dextrose agar cooling；
Step 2）：Prepare hypha suspension of the mother seeds；
The high-quality healthy and strong parent species being separately cultured from tissue, aseptically, expanded with Sterile pipette or syringe
Injection sterilized water in parent species test tube, mixing broken growth with transfer needle has by the culture medium of expansion parent species, and hypha suspension of the mother seeds is made；
Step 3）：Expansion connects；
Aseptically, hypha suspension of the mother seeds 5mL is extracted with Sterile pipette or syringe, in each step 1）Prepare
The expansion for filling improvement potato dextrose agar connects injection 0.05mL hypha suspension of the mother seeds in test tube and balanced；
Step 4）：Culture；
Expansion is connect into test tube and is put into incubated in constant incubator, cultivation temperature is stable at 22-28 DEG C, and 6-8 days mycelia of culture are
130mm test tube slant culture medium can be covered with.
2. the quick propagation method of Termitomyces albuminosus with black skin parent species according to claim 1, it is characterised in that the step 1.2）
It is middle improvement potato dextrose agar preparation method be：Potato is first cleaned into peeling, then weighs 150g choppings, is added
After water 1000mL is boiled by slow fire 20-30min, with four layers of filtered through gauze, soluble alcohol grain 100-150g, glucose are added
20g, potassium dihydrogen phosphate 2g, magnesium sulfate 0.5g, agar 20g, yeast extract 2-3g and VB15-10mg, continue heating stirring and mix
Melt to agar, then supply water to 1000mL, be well mixed, culture medium is dispensed to test tube when temperature is not less than 70 DEG C and grown
The 1/4-1/5 of degree, test tube mouth add cotton carding plug, are then tied up with brown paper outside test tube mouth tampon.
3. the quick propagation method of Termitomyces albuminosus with black skin parent species according to claim 1, it is characterised in that the step 1.3）
Middle sterilization process is：The triangular flask for filling water and the test tube for filling improvement potato dextrose agar are loaded into high pressure
Pot, autoclaving 30min under 0.11-0.12MPa.
4. the quick propagation method of Termitomyces albuminosus with black skin parent species according to claim 1, it is characterised in that the step 2）In
The tissue of parent species is separately cultured process and is cured to extract tender body cell induction from the Termitomyces albuminosus with black skin trophosome grown
Injured tissue, mycelium is gradually trained, and chooses pure white, healthy and strong mycelium as parent species.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711229362.8A CN107801568A (en) | 2017-11-29 | 2017-11-29 | A kind of quick propagation method of Termitomyces albuminosus with black skin parent species |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711229362.8A CN107801568A (en) | 2017-11-29 | 2017-11-29 | A kind of quick propagation method of Termitomyces albuminosus with black skin parent species |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107801568A true CN107801568A (en) | 2018-03-16 |
Family
ID=61581840
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711229362.8A Withdrawn CN107801568A (en) | 2017-11-29 | 2017-11-29 | A kind of quick propagation method of Termitomyces albuminosus with black skin parent species |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107801568A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110754292A (en) * | 2019-11-25 | 2020-02-07 | 广东省微生物研究所（广东省微生物分析检测中心） | White variety of oospore oudemansiella mucida and artificial cultivation method thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101218876A (en) * | 2007-12-26 | 2008-07-16 | 孙思国 | Termitomyces albuminosus with black skin producing process |
| CN102523915A (en) * | 2011-12-15 | 2012-07-04 | 滨州职业学院 | Fast propagation method for single-type hypha mother seeds of edible fungi |
| CN104285663A (en) * | 2013-07-16 | 2015-01-21 | 曲淑敏 | Black termitomyces cultivation method |
| CN104885788A (en) * | 2015-06-19 | 2015-09-09 | 福建万亿店中店电子商务有限责任公司 | Black skin termitomyces albuminosus mycelium culture method |
| CN105695338A (en) * | 2016-01-25 | 2016-06-22 | 四川保兴现代农业科技股份有限公司 | Liquid spawn propagation method and field bionic cultivation method of termitomyces albuminosus |
-
2017
- 2017-11-29 CN CN201711229362.8A patent/CN107801568A/en not_active Withdrawn
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101218876A (en) * | 2007-12-26 | 2008-07-16 | 孙思国 | Termitomyces albuminosus with black skin producing process |
| CN102523915A (en) * | 2011-12-15 | 2012-07-04 | 滨州职业学院 | Fast propagation method for single-type hypha mother seeds of edible fungi |
| CN104285663A (en) * | 2013-07-16 | 2015-01-21 | 曲淑敏 | Black termitomyces cultivation method |
| CN104885788A (en) * | 2015-06-19 | 2015-09-09 | 福建万亿店中店电子商务有限责任公司 | Black skin termitomyces albuminosus mycelium culture method |
| CN105695338A (en) * | 2016-01-25 | 2016-06-22 | 四川保兴现代农业科技股份有限公司 | Liquid spawn propagation method and field bionic cultivation method of termitomyces albuminosus |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110754292A (en) * | 2019-11-25 | 2020-02-07 | 广东省微生物研究所（广东省微生物分析检测中心） | White variety of oospore oudemansiella mucida and artificial cultivation method thereof |
| CN110754292B (en) * | 2019-11-25 | 2021-08-17 | 广东省微生物研究所（广东省微生物分析检测中心） | White variety of oospore oudemansiella mucida and artificial cultivation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104351901B (en) | A kind of probiotics fermention chlorella drink and preparation method thereof | |
| CN105532266A (en) | Termitomyces albuminosus cultivation method | |
| CN106561251A (en) | Planting method for high-yield and high-quality radix puerariae | |
| CN108558465A (en) | A kind of hickory chick exogenous nutrition material and its preparation method and application | |
| CN102835249A (en) | Cultivation method for organic coprinus comatus and culture medium | |
| CN107750814A (en) | A kind of breeding method of Wild ganoderma strain | |
| CN114946657B (en) | Hispid fig tissue culture method | |
| CN108029458A (en) | A kind of preparation method of Termitomyces albuminosus with black skin liquid spawn | |
| CN107227262A (en) | The breeding method of hickory chick strain | |
| CN107173058A (en) | A kind of method of utilization pepper bar mushroom culture | |
| CN107801568A (en) | A kind of quick propagation method of Termitomyces albuminosus with black skin parent species | |
| CN103283593A (en) | Dendrobium officinale tissue culture seedling method | |
| CN100399885C (en) | Method for promoting common shrimp ovary maturity and spawning | |
| CN104629983A (en) | Method for preparing fortified jujube wine | |
| CN102845306A (en) | Tissue culture breeding method of Acanthopanax senticosus | |
| CN102523915B (en) | Fast propagation method for single-type hypha mother seeds of edible fungi | |
| CN110663454A (en) | Artificial cultivation method of black ginseng | |
| CN109874676A (en) | A kind of fast breeding method of passion fruit detoxic seedling | |
| CN109329059A (en) | A kind of method of straw short-tube lycoris tissue cultures | |
| CN109169416A (en) | Pelteobagrus fulvidraco offspring seed cultivation method | |
| CN109220478A (en) | A kind of artificial method for planting of medicinal pilose actinodaphne bark or leaf | |
| CN103947824A (en) | Fungal multifunctional feed additive and its production method | |
| CN104818247B (en) | The cultural method and purposes of a kind of mescenchymal stem cell | |
| CN108419604A (en) | A kind of artificial kind of method for growing mushroom | |
| CN101953267A (en) | Method for culturing hepialus armoricanus oberthur cordyceps sinensis |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WW01 | Invention patent application withdrawn after publication | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20180316 |