CN107517965B - Eight-character cutworm attractant composition and application thereof - Google Patents

Abstract

The invention discloses a cutworm attractant composition and application thereof, belonging to the technical field of insect sex pheromone preparation. The lure composition of S. chinensis includes sex pheromone and synergist, the weight ratio of S. chinensis sex pheromone and synergist is 1: (0.5~100), wherein, the main component of S. chinensis sex pheromone For cis-7-tetradecene acetate, cis-5-tetradecene acetate and trans-5-tetradecene acetate, the synergist is selected from plant volatile substances: 1-hexanol , one or more of phenylacetaldehyde, cis-3-hexene-1-alcohol, heptanal, linalool. The attractant composition provided by the invention can be accurately applied to the insect situation forecasting, trapping and interfering with mating, and has great significance for comprehensively controlling the Shrimp cutthroat.

Description

A kind of cutworm lure composition and application thereof

technical field

The invention relates to the technical field of insect sex pheromone preparation, in particular to an attractant composition of cutworm and application thereof.

Background technique

Xestia c-nigrum (Linnaeus), belonging to Lepidoptera, Noctuidae, is a major agricultural and grassland cutting root pest. Larvae with suspended animation, nocturnal and strong concealment. Distributed all over the world, it is seriously harmful in the United States, Japan and other countries. It can harm tobacco, tomato, cabbage, grapes and other food habits. In my country, it mainly harms food crops, cotton, tobacco, vegetables, medicinal plants and more than 100 kinds of plants. , is famous for endangering the seedlings of a variety of plants, and its geographical distribution is all over the country. At present, the main methods of its control are spraying chemical agents, trapping and killing adults. The long-term use of chemical agents to control cutworms may make the pests resistant to pesticides, kill natural enemies, pollute the environment and destroy the ecosystem, and the control effect is unstable. There is no effective method to fundamentally control its occurrence.

More and more attention has been paid to the application research of insect semiochemical substances at home and abroad, and more semiochemical substances of insect pests have been continuously identified, synthesized and applied in the field. Although sex pheromones have the advantages of strong selectivity, high efficiency in trace amounts, and environmental friendliness, they can only trap and kill male individuals of the same species, and can achieve better results for species with a single mating habit. effect.

SUMMARY OF THE INVENTION

The purpose of the present invention is to provide an attractant composition for Siberian cutworm, to reduce the dosage of pheromone, improve the effect of comprehensive treatment of cutworm cutworm, and reduce the cost of prevention and treatment.

The lure composition of S. chinensis provided by the present invention comprises sex pheromone and synergist, and the weight ratio of the sex pheromone and synergist is 1:(0.5-100), wherein the S. chinensis sex pheromone and synergist are The main components of cutworm sex pheromone are cis-7-tetradecene acetate, cis-5-tetradecene acetate and trans-5-tetradecene acetate. The synergist group The components are plant volatile substances that are active on the cutworm, and the synergist contains at least one of 1-hexanol, phenylacetaldehyde, cis-3-hexen-1-ol, heptanal, and linalool. kind.

Preferably, the weight ratio of the sex pheromone of S. chinensis to the synergist is 1:(1-10).

Optionally, the synergist is composed of the following components according to their respective parts by weight: 0-9 parts of 1-hexanol, 0-9 parts of phenylacetaldehyde, 0-9 parts of cis-3-hexen-1-ol, 0 to 9 parts of heptanal and 0 to 9 parts of linalool.

Preferably, the synergist is composed of the following components according to their respective weight parts: 1-9 parts of 1-hexanol, 0-5 parts of phenylacetaldehyde, 1-9 parts of cis-3-hexen-1-ol, 1 to 9 parts of heptanal and 0 to 5 parts of linalool.

Preferably, the synergist is composed of the following components according to their respective weight parts: 2-5 parts of 1-hexanol, 1-3 parts of phenylacetaldehyde, 1-5 parts of cis-3-hexen-1-ol, heptyl alcohol 3 to 5 parts of aldehyde and 1 to 3 parts of linalool.

Optionally, the sex pheromone of Siberian cutis is composed of the following components according to their respective weight parts: 8-9 parts of cis-7-tetradecene acetate, 0.1 parts of cis-5-tetradecene acetate ~1 part and 0.1-0.5 part of trans-5-tetradecene acetate.

Preferably, the sex pheromone of the cutworm is composed of the following components according to their respective weight parts: 8.5-9 parts of cis-7-tetradecene acetate, 0.5 parts of cis-5-tetradecene acetate ~1 part and 0.1-0.3 part of trans-5-tetradecene acetate.

Preferably, the sex pheromone of C. chinensis consists of the following components according to their respective weight parts: 9 parts of cis-7-tetradecene acetate, 0.8 parts of cis-5-tetradecene acetate and trans- -0.2 part of 5-tetradecene acetate.

The present invention also provides a method for using the Shrimp cutis lure composition, which comprises applying the synergist to the lure core containing the sex pheromone of S. chinensis; or combining the synergistic agent with the sex pheromone of S. chinensis After mixing, it is applied to the blank lure core; or the synergist is applied to the blank lure core, and then the lure core containing the synergist is used in combination with the sex pheromone of Siberian cutworm.

The present invention also provides a lure core for trapping Shrimp cutthroat, wherein the lure core contains the above-mentioned Shrimp cutthroat lure composition.

The present invention also provides the above-mentioned preparation method of the lure core for trapping the cutworm, comprising: adding the n-hexane solution containing the synergist to the lure core containing the sex pheromone of the cutworm, and after the n-hexane volatilizes Dichloromethane is added dropwise to the lure, and after the dichloromethane is volatilized, the lure containing the sex pheromone and its synergistic agent of the cutworm is prepared. The prepared lure composition can be enclosed in aluminum foil, sealed, and stored at -20°C.

The present invention also provides a method for controlling and/or monitoring S. serratus, comprising applying the above-mentioned S. serratia attractant composition to the habitat of S. serratia.

Optionally, the dosage of the cutworm attractant composition is 0.1-10 mg/square meter.

Preferably, the dosage of the cutworm attractant composition is 0.5-2.5 mg/square meter.

Beneficial effects of the present invention: 1) The use mode is flexible, and the present invention provides a Shrimp cutis lure composition, including sex pheromone and a synergist thereof, which can be made into a synergist lure core and a cutworm sex pheromone lure. When used in conjunction with the core, the sex pheromone solution and its synergist solution can also be mixed to make a lure composition to lure the core; a variety of components can be freely selected to be used alone, or used together according to the ratio, which is more autonomous. 2) Compared with the lure core containing only the sex pheromone of S. chinensis without its synergist, the use of the lure containing the sex pheromone of S. 3) The synergist component provided by the present invention is low in price, can reduce the cost of making the lure core, and is suitable for popularization and promotion.

Detailed ways

The specific embodiments of the present invention are described in detail below, but it should be understood that the protection scope of the present invention is not limited by the specific embodiments.

The information such as the purity and supplier of the sex pheromone components and plant volatiles components used in the following examples are shown in Table 1.

Table 1 Sex pheromone components and synergistic components of S. chinensis

Example 1 Y-type olfactometer determination of single-component synergist

Preparation of a single dose of plant-derived volatiles: each compound was prepared with n-hexane as a solvent, and prepared into 3 concentrations of 0.1, 1, and 10 μg/μl, and n-hexane was used as a control.

The sensitivity of S. chinensis to various reagents was determined by "Y"-type olfactometer. In a room with a relative humidity of about 70% and clean air at 25°C, a 3cm*20cm "Y"-type olfactometer was used for bioassays. 10 μl of the single-component compound of the synergist was dropped on a filter paper strip, placed in one side arm of the Y-type olfactometer, and an equal amount of n-hexane was placed in the other side arm as a control. After each group was repeated, the positions of the experimental group and the control group were reversed to avoid experimental errors. A new filter paper strip was replaced after each test, each reagent was repeated 3 times, and a clean "Y"-shaped tube was required to be replaced for each reagent. A cutworm that had been fasted for 24 hours was placed 3cm away from the main arm of the Y-type olfactometer. If the adult cutworm stayed in either arm for more than 1 min, it was considered that a choice had been made; l0min, it is considered that there is no response. Repeat 30 cutworms, 15 males and 15 males. After 10 minutes, the distribution of the test insects was observed and the response rate, relative response rate and response coefficient were calculated for data analysis.

It can be seen from Table 2 that the response rates of the selected test worms are all above 80%, and there is no significant difference between the treatments, indicating that the selected test worms are healthy, and the experimental results of this study are reliable. From the selection response rate, it can be seen that the response values of S. chinensis to different concentrations of the same synergist are not significantly different. For the same synergist, the response values of males and females were not much different. The selective response rate of S. chinensis to cis-3-hexen-1-ol was the highest (0.83±0.04 for males and 0.84±0.06 for females), which was significantly higher than other synergists at different concentrations. The single-component synergists have the attracting activity to S. chinensis adults. Among them, cis-3-hexen-1-ol, heptanal and 1-hexanol have similar attracting activities to S. serratus, phenylacetaldehyde and linalool The attractant activity is relatively poor.

Table 2 The olfactory responses of cutworms to one-component synergists

The data in the table are mean ± standard error. Different letters after the data in the same column indicate significant differences at the P<0.01 level by Duncan's new multiple range test.

Embodiment 2 The synergistic effect of different formula synergists on sex pheromone of S. chinensis

1-hexanol, phenylacetaldehyde, cis-3-hexen-1-ol, heptanal, linalool and sex pheromone were prepared into 500 μg/μL n-hexane solution, respectively. Among them, the sex pheromone of the cutworm is 9 parts of cis-7-tetradecene acetate, 0.8 parts of cis-5-tetradecene acetate and 0.2 parts of trans-5-tetradecene acetate. Mixed.

Pipette a certain amount of each synergist component solution and the sex pheromone solution of S. chinensis with a 200 μL pipette and mix them evenly to obtain a mixture of S. chinensis plant-derived pheromone. The ingredients of the attractant composition are shown in Table 1.

Use a 200μL pipette to pipette 200μL of the Shrimp cutis lure composition or the sex pheromone of S. chinensis onto the septum of the rubber stopper washed with n-hexane. After the n-hexane is volatilized, 1 ml of dichloromethane is added to the droplets. Add, after the dichloromethane is volatilized, the lure core of the cutworm lure composition or the sex pheromone lure of the cutworm is prepared. Each lure core contains 100mg of S. chinensis lure composition or sex pheromone of S. chinensis. Pack the prepared lure into aluminum foil, seal it, and store it at -20°C.

In the field trapping experiment, the trapping core of the Shrimp cutworm composition or the sex pheromone lure core of the cutworm cutworm is fixed to the universal moth trap with a thin wire. CK1 is the blank control without the lure of S. chinensis composition or the sex pheromone lure of S. chinensis; CK2 is the control of S. chinensis sex pheromone lure only; groups X1 to X5 are only single-component synergistic The treatment of the lure core; Group 01 to 15 are the treatment of the lure core with the single-component Shrimp cutthroat lure composition; Group 16 to 42 are the Shrimp cutthroat lure composition lure with two-component synergist. Treatment of cores; Groups 43 to 51 are the treatment of cores with three-component synergist-containing Shrimp cutthroat lure composition; 52-57 groups are the four-component synergist-containing Shrimp cutthroat lure composition lure. Treatment of the core; Groups 58 to 65 are the treatment of the core of the cutworm lure composition containing the five-component synergist. Three replicates were set up for each treatment, randomly arranged, and two adjacent traps were separated by 10m. From the second day after the setting, the number of trapped cutworms was recorded every morning and removed in time, and the investigation continued for 30 days.

The test results are shown in Table 3. It can be seen from Table 3 that the single-component synergist has the attracting activity to S. chinensis, but the trapping effect is obviously lower than the trapping effect of the sex pheromone of S. chinensis itself. However, when the single-component synergist was mixed with the sex pheromone of S. chinensis, the trapping effect was significantly higher than that of the single-component synergist alone. When the synergist and the sex pheromone are mixed in different formula ratios, the trapping effect of the sex pheromone lure core is higher than the trapping effect of the sex pheromone itself, and the amount of the sex pheromone is greatly reduced.

Table 3 Field synergistic effects of different components of synergists on sex pheromone of S. chinensis

The attractant composition provided by the invention can be accurately applied to the insect situation forecasting, trapping and interfering with mating, and has great significance for comprehensively controlling the Shrimp cutthroat. In addition, the S. chinensis attractant composition is simple to prepare, easy to use, feasible in conditions, has strong trapping ability and good stability; at the same time, the S. chinensis attractant composition has strong specificity, does not produce resistance, and is resistant to Natural enemies are not harmful, non-toxic to humans and animals, and environmentally friendly; strong resistance to stress, not afraid of wind, rainforest and sunlight; long-lasting effect, the control effect can be maintained for more than 30 days.

The attractant composition of the present invention is made from sex pheromone and plant volatile substances, and is characterized in that (1) the sex pheromone itself has the advantages of high efficiency, low toxicity and no pollution; (2) it is based on the host Spodoptera frugiperda attractants made from plant volatiles are difficult to make Spodoptera frugiperda insects resistant to pesticides; (3) Compared with traditional pesticides, the amount of Spodoptera frugiperda attractants is very small, and only a very small amount can produce biological Activity; (4) Wide spectrum of attracting insects. According to the identification of host plant volatiles by insects and the principle that female insects release sex pheromones with strong attracting ability to males of the same species, it is clear that the formula ratio between female sex pheromone and plant volatiles can be accurately applied It can be used for insect forecasting, pest quarantine, interfering with mating, and mass trapping, so as to achieve the ideal effect of comprehensive management of the cutworm.

Those of ordinary skill in the art should understand: the above are only specific embodiments of the present invention, and are not intended to limit the present invention. Any modification, equivalent replacement, improvement made within the spirit and principle of the present invention etc., should be included within the protection scope of the present invention.

Claims (5)

1. The attractant composition for attracting the black cutworm is characterized by comprising 100 mu L of black cutworm sex pheromone and a synergist, wherein the black cutworm sex pheromone comprises the following components in parts by weight: 9 parts of cis-7-tetradecene acetate, 0.8 part of cis-5-tetradecene acetate and 0.2 part of trans-5-tetradecene acetate; the synergist comprises 20 mu L of 1-hexanol, 10 mu L of phenylacetaldehyde, 30 mu L of cis-3-hexene-1-alcohol, 30 mu L of heptaldehyde and 10 mu L of linalool.

2. A lure for trapping black cutworm according to claim 1, comprising the black cutworm lure composition according to claim 1.

3. The method of using a composition of black cutworm lure according to claim 1 including applying the synergist to a lure containing black cutworm sex pheromone; or the synergist is mixed with the tiger sex pheromone and then applied to a blank lure core; or the synergist is applied to a blank lure core, and the lure core containing the synergist is matched with the tiger sex pheromone for use.

4. A method for controlling and/or monitoring black cutworm, comprising applying the black cutworm attractant composition of claim 1 to the habitat of black cutworms.

5. The method for controlling and/or monitoring black cutworm according to claim 4, wherein the amount of the black cutworm attractant composition is 0.1 to 10 mg/m.