CN107494976A - A kind of layer dietses of the polyunsaturated fatty acid eggs of rich ω 3 and preparation method thereof - Google Patents
A kind of layer dietses of the polyunsaturated fatty acid eggs of rich ω 3 and preparation method thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
- A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/158—Fatty acids; Fats; Products containing oils or fats
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- A23K20/10—Organic substances
- A23K20/174—Vitamins
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- A—HUMAN NECESSITIES
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- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/189—Enzymes
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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Abstract
The invention discloses a kind of layer dietses of the rich polyunsaturated fatty acid eggs of ω 3, mainly it is prepared from the following raw materials in parts by weight：10 20 parts of barley, 5 10 parts of fermented bean dregs, 24 parts of brewer's yeast, 5 10 parts of expanded soybean, expanded 25 parts of linseed, 36 parts of oat bran, 36 parts of Alfalfa, 25 parts of super-micro wall-broken grape pip, 28 parts of seawood meal, 0.5 part of compound premix, 0.003 0.005 parts of yeast selenium, 0.05 0.1 parts of complex enzyme formulation, 0.03 0.08 parts of glycine betaine, 0.05 0.1 parts of probiotic, it is 0.3 part to win kind X 1,0.01 0.03 parts of phytase, 0.03 0.1 parts of lipase, vitamin, amino acid etc..The daily ration, which is used for breeding layer chicken, can improve immunity of layer chicken, premunition, improve the polyunsaturated fatty acids of ω 3, particularly DHA content in egg.
Description
Technical field
The present invention relates to a kind of production method of egg, particularly one kind to be rich in omega-3 polyunsaturated fatty acids, especially
The layer dietses and its production method of DHA egg
Background technology
(Docosahexaenoic acid, i.e. docosahexaenoic acid, one kind contain 22 carbon atoms and 6 double bonds to DHA
Straight chain fatty acid), " docosapentaenoic acid " of human brain；DHA is the important composition composition of brain and retina, has enhancing to remember,
Improve intelligence and improve the effect of eyesight.Research finds that DHA can also adjust blood fat, reduces blood viscosity, prevention miocardial infarction and
Cerebral thrombus, and there is anti-inflammatory, anticancer.But it can not be synthesized by human body, can only be absorbed from food, such as three texts
Contain a small amount of DHA in fish, tuna, seaweed food.Omega-3 unsaturated fatty acid means some obtained by extraction in fish oil
Active material, its Representative Cultivars have EPA, DHA and DPA etc..Carry out medical researchers' confirmation in the past few decades:Take unsaturated lipid
Fat acid can effectively reduce coronary heart disease (myocardial infarction) death rate, and the U.S. is universally acknowledged coronary heart disease big country, therefore from 20th century
Since the nineties, the functional food containing omega-3 unsaturated fatty acid has gone up to be in great demand greatly functional food for the whole America first.Such as according to U.S.
The latest report of state's domestic media, the various functions food added with omega-3 unsaturated fatty acid composition is total on last year American market
Sales volume is more than 7,000,000,000 dollars.The linseed oil sales volume extracted in linseed rises rapidly, because in terms of pharmacology angle, flax
The effect of extremely abundant gamma-Linolenic acid contained by oil is quite similar with the unrighted acid from fish oil.In metabolic process
In can from alpha linolenic acid generate DHA.Research is rich in DHA eggs, many Patent No. of reporting for work rich in DHA milk both at home and abroad
CN105581012A《A kind of production method rich in DHA eggs》, disclose using lactic acid bacteria, methionine, zinc sulfate, grape
Sugar, vitamin B6, vitamin K, vitamin C, fat, yellow meal worm powder, hawthorn powder, calcium lactate, flaxseed meal, camellia seed meal, dish
The dregs of rice and earthworm powder etc., the method for producing DHA eggs, the few cost of the patent raw material sources is high, and DHA content is low in raw material, deposition
The low problem of rate；Patent No. CN102696869B《Method for producing egg rich in DHA and vitamin E》Disclose and use corn
500-650, fodder maize albumen powder 15-20, rice bran meal 20-30, corn DDGS 20-50, wheat bran 20-30, stone flour 65-70,
Calcium monohydrogen phosphate 5-15, fish oil 15-100, linseed 30-100, salt 2-4, methionine 0.8-1, vitamin premix 0.3-0.5,
The happy B io-Aid0.5-1 of adsorptivity vitamin E powder 0.1-0.5, microelement pre-mix material 1-1.5, Choline Chloride 1-2, beneficial bacteria, section
For enzyme 0.25-0.5, phytase 0.1-0.2, compound betaine 0.2-0.4, egg is received after three weeks.The standby DHA eggs of the patent system contain
There are a large amount of fish oil, the inferior position that egg fishy smell is big, and commodity egg shelf life section etc. can not overcome easily be present.Patent No.
CN102715382A《Feed adjusting control agent of the production rich in DHA eggs and the feed containing the adjusting control agent》, adjusting control agent is disclosed as purple
Perillaseed 24-36%, pumpkin 4-12%, spirulina 8-16%, fish meal 8-14%, sea sedge 3-7%, wind-weed 2-6%, multidimensional 3-
10%th, fish oil 1-3%, bighead atractylodes rhizome 4-9%, matrimony vine 3-7%, metallothionein slightly carry powder 0.01-0.03%, methionine 3-6%, pine
Pin powder 5-13%, oyster shell whiting 3-6%；The addition of the feed adjusting control agent is the 1-8% of feed gross weight, and the patent uses a large amount of
Traditional Chinese medicine ingredients, resource is few, cost is high, is unfavorable for specialized Industry Promotion application, and Chinese medicine is without processing in laying hen material
Middle application effect is poor, is analyzed according to composition and addition, and DHA content is low in egg.
It is the feed patent of production function egg below, Application No. CN200710115163.4《Produce health-care eggs
Egg feedstuff》, using the selenium of routine, vitamin E, bacillus subtilis and Chinese herbal medicine (Radix Astragali, Radix Codonopsis, wilsonii, barrenwort
Deng), it is to be developed from the feed regimen for reducing cholesterol, is not in contact with DHA egg feeds of the present invention.Application No.
CN201510022552.7《A kind of feed addictive of layer chicken rich in omega-3 polyunsaturated fatty acids》Using the Asia of routine
Numb seed and enzyme preparation, omega-3 polyunsaturated fatty acids average content is 750 milligrams in 100 grams of eggs；This patent Main way is
Egg of the production rich in DHA, has used fish oil, linseed and expanded soybean etc. to be rich in the raw material of omega-3 polyunsaturated fatty acids,
Therefore ω -3 and ω -6 1670 milligrams/100 grams eggs of unrighted acid are deposited in egg.Application No.
CN201710127302.9《Egg rich in omega-3 polyunsaturated fatty acids and Co-Q10 extremely preparation method》, this patent
Main direction of studying is omega-3 polyunsaturated fatty acids and Co-Q10, the target and solution different from this patent.Application number
For CN20100002265.7《Feed addictive of layer chicken and its production method and application》, Enriching Selenium mainly in egg, zinc and
Omega-3 polyunsaturated fatty acids, it is different from this patent main direction of studying.Application No. CN201310117021.7《One kind is rich in
The production method of the layer dietses and the egg of omega-3 polyunsaturated fatty acids and lotus leaf active material》, mainly ground with this patent
It is different with research method to study carefully direction.
Egg nutrient enriches, and eating method is various, but the fishy smell of egg prevents many people from receiving.The fishy smell master of egg
It is relevant with trimethylamine, when content is higher than 1mg/Kg to trimethylamine in egg, it is possible to substantially perceive fishy smell.
Therefore, it is high that a kind of omega-3 polyunsaturated fatty acids content height, DHA depositions are invented, the low easily popularization of cost, egg product wind
The good shelf life length of taste is rich in DHA eggs, and the feed of the rich DHA eggs of production, is that brand egg and breeding layer chicken upgrading increase
Effect creates bigger economic value, is our problems to be solved.
The content of the invention
To solve the above problems, the present invention is started with from rich in the selection of DHA raw materials and additive using grade comprehensive nutrient measure,
Make every effort at utmost reduce DHA egg production costs, DHA in egg is maximized deposition, extend the shelf life of commodity egg.Root
Daily ration is designed according to the week old of week old -50 of laying cycle of laying hens 20 nutritional need；Selection raw material simultaneously combines according to special ratios, with enhancing
DHA deposition capabilities and omega-3 unsaturated fatty acid deposition, enhancing laying hen immunologic function and oxidation resistance, Egg Quality is improved,
It is main target to extend commodity egg shelf life and egg product security, and science compounding one kind is rich in DHA and omega-fatty acid egg
Least cost feed.
In order to achieve the above object, the present invention uses following technical scheme：
A kind of layer dietses of rich omega-3 polyunsaturated fatty acids egg, are prepared from the following raw materials in parts by weight：Barley
10-20 parts, corn 30-40 parts, fermented bean dregs 5-10 parts, corn protein powder 5-10 parts, brewer's yeast 2-4 parts, expanded soybean 5-
10 parts, expanded linseed 2-5 parts, oat bran 3-6 parts, Alfalfa 3-6 parts, super-micro wall-broken grape pip 2-5 parts, fish oil 2-4
Part, fish meal 1-3 parts, seawood meal 2-8 parts, stone flour 8-10 parts, calcium monohydrogen phosphate 0.5-0.8 parts, salt 0.2-0.4 parts, amino acid
0.35-1.0 parts, 0.5 part of compound premix, yeast selenium 0.003-0.005 parts, vitamin E 0.01-0.02 parts, vitamin C
0.02-0.05 parts, complex enzyme formulation 0.05-0.1 parts, Choline Chloride 0.05-0.12 parts, glycine betaine 0.03-0.08 parts, probiotic
0.05-0.1 parts, it is 0.3 part to win kind rich element X-1, phytase 0.01-0.03 parts, lipase 0.03-0.1 parts.
Further, the amino acid is lysine, methionine, threonine and tryptophan in mass ratio 1：0.7-1.5:
0.5-1.0:0.1-0.5 is uniformly mixed.
Further, the compound premix：Liaoning Hefeng Animal Husbandry Co., Ltd produces, and meets company standard Q/HF
J02.03-2015, product authentication code raise pre- word (2013) 003024 for the Liao Dynasty；Product form is B B-complex and micro member
Element, trade mark is that standing grain is rich, commercially available.Compound premix composition is as follows：Iron 15000mg/Kg, copper 3000mg/Kg, zinc 20000mg/
Kg, manganese 24000mg/Kg, selenium 60mg/Kg, iodine 100mg/Kg, vitamin A 1600KIU, vitamin D 600KIU, vitamin
E320mg/Kg, vitamin K3200mg/Kg, vitamin B1200mg/Kg, vitamin B2880mg/Kg, vitamin B6600mg/
Kg, vitamin B122.0mg/Kg, nicotinic acid 6000mg/Kg, pantothenic acid 800mg/Kg, folic acid 100.0mg/Kg, biotin 5.5mg/Kg；
Further, the phytase：It is raw for 10,000 unit phytases of Beijing Smistyle Science ＆ Technology Development Co., Ltd.'s production
Production credit number is that feeding adds (2012) 0017, authentication code：Raise (adding) word (2012) 403711 in capital；Main component is phytase；
It is commercially available；
Further, the complex enzyme formulation：Shenyang Fengmei Biotechnology Co., Ltd. produces, and production licence number adds for feeding
(2011) 1986, authentication code：(adding) word (2011) 040008 is raised by the Liao Dynasty；Contents of Main Components is as follows：Zytase 3000U/g,
Mannase 800U/g, acid protease U/g, lipase 800U/g, amylase 300U/g, dextranase 400U/g.Market
It is on sale；
Further, the probiotic is mannosan, Fructooligosaccharides, zymosan, bacillus coagulans, lichens gemma bar
At least one of bacterium, saccharomycete, lactic acid bacteria；
Further, the probiotic is 3-6 comprising parts by weight:1.5-3.5:2.3-5.5 mannosan, few fruit
Sugar, zymosan；
Further, the living bacteria count in the probiotic is：Bacillus coagulans >=11,000,000,000/g, lichens gemma bar
Bacterium >=100,000,000,000/g, saccharomycete >=12,000,000,000/g, lactic acid bacteria >=6,000,000,000/g；
Further, the bacillus coagulans (Bacillus coagulans) is the condensation gemma with antibacterial activity
Bacillus, is an isolated bacillus from health-care eggs chicken intestinal, and its zymotic fluid has good to C.perfringens
Antibacterial activity, through Physiology and biochemistry and genotype identification, the bacterial strain is bacillus coagulans, is preserved in Chinese microorganism strain preservation
Administration committee's common micro-organisms center, deposit number are CGMCC NO.10221.
Further, ultramicro grinding grape pip disintegrating process is to crush grape pip through 200 mesh, with bentonite, wheat bran
With 90:2:8 ratio carries out low temperature granulation.
A kind of preparation method of the layer dietses of rich omega-fatty acid egg, comprises the following steps：
Each component raw material accurately is weighed according to formula, is crushed according to conventional egg feedstuff processing method, then by weight
Number is arrogant to small to sequentially add various raw materials, is well mixed, processing, produces one kind and is rich in omega-3 polyunsaturated fatty acids egg
Layer dietses.
Beneficial effect
The present invention significantly improves fat digestibility using lipase and special probiotics, improves in egg
DHA accumulation ability；Lipase is a kind of special ester linkage hydrolyzing enzyme, and it may act on the ester bond of triglycerides, makes glycerine
Three esters are degraded to diglyceride, monoglyceride, glycerine and aliphatic acid., can using multi-cultur es source, the lipase through solid fermentation
Digestibility is reached with the fully emulsified decomposition of crude fat in daily ration and improves more than 10%, improves alimentary canal health status, enhancing
Immunity.
Vitamin E and yeast selenium all have antioxidation, add polyphenoils yeast selenium in feed or vitamin E can
Average egg weight, yolk color and the hangh unit and eggshell strength of laying hen under oxidative stress status are improved, improves egg quality, also
Laying hen GSH-Px and SOD activity under oxidative stress status can be improved, the content of MDA in serum is reduced, improves the anti-oxidant energy of body
Power.Most of research shows yeast selenium and vitamin E while acted synergistically using having, while the effect added adds better than single
Add.
Glycine betaine (Betaine, BET) also known as trimethylaminyl ethlyl lactone (Trimethylaminoacetate) or trimethyl are sweet
Propylhomoserin (N, N, N-Trimethylglycine).Because its unique chemical constitution, glycine betaine plays in living organism
Multiple functions.It can provide three unstable methyl, raw material be provided for many kinds of substance synthesis, such as carnitine, creatine, purine and egg
Propylhomoserin etc..Meanwhile glycine is generated through Demethylation, into amino acid metabolism pond.Because glycine betaine can provide methyl and ammonia
Base acid, it may participate in the processes such as body amino acid metabolism, fat metabolism and DNA methylation.Glycine betaine can promote liver and muscle
The synthesis of middle carnitine.Carnitine is that long chain fatty acids enter in zooblast mitochondria the unique transport vehicle for carrying out beta-oxidation, carnitine
Synthesis need lysine to provide synthesis skeleton, methionine then provides methyl, and glycine betaine can promote methionine to synthesize and reduce it
Decompose, be met the methyl of synthetic carnitine, and then adjust distribution of the fat in musculature and adipose tissue.Carnitine can carry
High chest muscle rate and muscle crude fat content and reduce abdominal fat, and improve liver, serum and chest muscle VBT content (Xu etc.,
2003；Du Rong etc., 2005).Meanwhile VBT can reduce the fat content of trunk, pathogenesis of fatty liver rate is reduced, improves protein
Deposition efficiency (Owen etc., 2001).Eklund etc. (2005) general part result of study thinks that glycine betaine can be by improving enteron aisle
The turn-over capacity of epithelial cell Na+ dependence passages promotes the absorption of Na+ dependence ions, while glycine betaine is as methyl donor
The synthesis of chylomicron can be promoted, improve the ability of hepatic transport fat, and then promote absorption of the animal to fat.According to report
The positive effect in terms of laying hen reduces fatty liver, anti-heat stress and improves egg white of an egg concentration of road glycine betaine.
Rich kind rich element X-1 main components are the compositions such as fermentation enzymolysis protein (small peptide), yeast cell wall, nucleotides, are had
There is enhancing animal oxidation resistance and mitigate inflammatory reaction effect, and then reduce trimethylamine concentration in egg, egg can be removed
Fishy smell, improve mouthfeel.
The immunity of layer chicken raised using laying hen material of the present invention is strong, and premunition is strong, and egg laying performance is good, and Egg Quality is good, egg
In be rich in omega-fatty acid, particularly DHA content.Omega-3 polyunsaturated fatty acids content reaches 586.5 milligrams/100 grams, ω -6
Content of polyunsaturated fatty acid reaches 1083.9 milligrams/100 grams, and wherein DHA content has reached 438.8 milligrams/100 grams, and leaf is yellow
Cellulose content improves 91.25%.The present invention takes aggregate measures to enhance the oxidation resistance of DHA eggs, significantly improves goods
The frame phase extends to 65.1 days.
Embodiment
The present invention is described below by specific embodiment.Unless stated otherwise, technological means used in the present invention
It is method known in those skilled in the art.In addition, embodiment is interpreted as illustrative, it is not intended to limit the present invention
Scope, the spirit and scope of the invention are limited only by the claims that follow.To those skilled in the art, without departing substantially from this
The various changes carried out on the premise of invention spirit and scope to the material component in these embodiments and dosage or change
Belong to protection scope of the present invention.
Bacillus coagulans (Bacillus coagulans), the bacterial strain have been stored in Chinese microorganism strain preservation management
Committee's common micro-organisms center, preserving number are CGMCC NO.10221, preservation address：BeiChen West Road, Chaoyang District, BeiJing City 1
Institute 3, Institute of Microorganism, Academia Sinica, postcode 100101.
Analysis of Antimicrobial Activity：Bacillus coagulans is lined into LB agar plates, 30 DEG C are incubated overnight rear picking single bacterium colony and connect
Plant in 10ml LB meat soups, 150rpm, 30 DEG C of culture 24h, 1000ml improvement tryptone meat soups be inoculated in by 0.2% inoculum concentration,
150rpm, 30 DEG C of culture 24h, 10000rpm centrifugation 15min obtain fermented supernatant fluid.By L.innocua,
L.monocytogenes, S.aureus, B.cereus, M.luteus in 37 DEG C of overnight incubations of LB agar lining out, choose respectively
Single bacterium colony is taken to be inoculated in LB meat soups, 37 DEG C are incubated overnight, and draw 10ul nutrient solutions and are inoculated in 10ml LB soft agars (containing 0.75%
The LB culture mediums of agar), LB agar plates are laid in after mixing, the Oxford cup of sterilizing is placed after cooling, 100 μ l is added and condenses gemma
Fermented supernatant fluid after bacillus fermentation supernatant or two times of gradient dilutions, 30 DEG C of overnight incubations, inhibition zone is observed, calculate bacteriocin
Potency.The potency of bacteriocin is determined using coubling dilution, is represented with AU/ml, specific as follows：By fermented supernatant fluid or bacteriocin
Crude extract carries out two times of gradient dilutions with sterile saline, and 100 μ l of each dilution gradient sampling, which are added in Oxford cup, to be detected
Antibacterial activity, the highest extension rate for inhibition zone occur are defined as an active unit, and it is original that its inverse, which is multiplied by extension rate,
The antibacterial activity potency (AU/ml) of liquid.The fermentation of bacillus liquid is to gram-positive bacterium such as C.perfringens, golden yellow
Staphylococcus, bacillus cereus, Listeria etc. have stronger antibacterial activity.
Class bacteriocin stability：1. to the tolerance of temperature：Fermented supernatant fluid is located respectively at 60,70,80,90,100 DEG C
10min is managed, using not thermally treated sample as control, L.innocua is indicator bacteria, shadow of the detection heat treatment to bacteriocin activity
Ring, antibacterial activity Retention represented with the ratio between the antibacterial circle diameter for the treatment of group and control group antibacterial circle diameter,；60-100 DEG C of difference
Processing 10 minutes, bacteriocin activity is substantially uninfluenced, and activity is held at more than 90%.
2. to the sensitiveness of enzyme：Pepsin, trypsase, pronase, fat are separately added into fermented supernatant fluid
Fat enzyme, amylase, the final concentration of 1mg/ml of enzyme, pepsin pH are 2.5, and other ferment treatment pH are 6.5,37 DEG C of processing
After 2h, the Retention of antibacterial activity is detected using L.innocua as indicator bacteria, untreated fermented supernatant fluid is control,；The condensation
Bacillus is insensitive to pepsin, trypsase, lipase and amylase, but the antibacterial after pronase ferment treatment 2h
Active Retention is 20%, and it is a kind of protein to illustrate Bacillus coagulans CGMCC NO.10221, can be by pronase
Degraded.
3. to the tolerance of soda acid：1ml bacteriocin crude extracts are taken respectively in test tube, with 0.5M hydrochloric acid or sodium hydroxide
PH to 2,3,4,5,6,7,8,9,10 are adjusted, pH is adjusted to 7.0 after standing 2h, using L.innocua as indicator bacteria, does not adjust pH's
Bacteriocin crude extract is control, detects antibacterial activity.
Such bacteriocin has certain tolerance to high temperature, and 60-100 DEG C is handled 10 minutes respectively, and antibacterial activity retains
More than 90%；Such bacteriocin is insensitive to pepsin, trypsase, lipase and amylase, but in pronase
Activity disappears after processing；Such bacteriocin keeps activity under neutral and acid condition, but in the basic conditions can loss section
Divide activity.
Molecular weight determination：In order to verify the protein attribute of Bacillus coagulans CGMCC NO.10221 bacteriocins, simultaneously
The molecular weight of the bacteriocin is determined, carries out SDS-PAGE experiments.SDS-PAGE resolving gel concentrations 16%, made with the bacteriocin of purifying
For electrophoresis Sample, applied sample amount 10ul, 60V electrophoresis 1h, 100V electrophoresis 2h, using low molecular weight protein standard items as control, electricity
Swim and glue is cut into two parts after terminating, the part coomassie brilliant blue staining comprising protein standard substance and bacteriocin sample, separately
A part only has bacteriocin sample, is put into fixer (25% aqueous isopropanol) and fixes 30 minutes, then washes 60 points with distillation
Clock.Washed glue is placed on LB agar plates, spreads inoculation L.innocua LB soft agars, will be flat after soft agar cooling
Plate is placed on 37 DEG C of culture 16h in incubator, observes antibacterial activity.As a result show, the bacillus coagulans produces in LB culture mediums
Raw class bacteriocin antibacterial material, molecular weight are about 4500 dalton, and 4) binding mode
LB culture medium 9ml, the L.innocua that 100 μ l of inoculation are incubated overnight, wherein one are separately added into two sterile tubes
Pipe adds bacteriocin crude extract 1ml, and another pipe adds sterile distilled water 1ml, places them in 37 DEG C of cultures, is taken out every 2h
1ml samples, detect L.innocua viable count.Listeria quantity after class bacteriocin and Listeria co-incubation is reduced,
Illustrate its binding mode for sterilization.
Growth curve：Bacillus coagulans CGMCC NO.10221 is connect in the flat lining out cultures of LB, picking single bacterium colony
Kind in 10ml LB fluid nutrient mediums, in 37 DEG C of overnight incubations, 1ml nutrient solutions are taken to be inoculated in 500ml LB liquid mediums, 37 DEG C,
200rpm cultivates 48h, samples detection zymotic fluid OD values and antibacterial activity every 4h, indicator bacteria is used as using L.innocua.Condense bud
Spore bacillus CGMCC NO.10221 enter exponential phase after 4h is cultivated, and 20h enters plateau, and 8h starts to produce class bacterium
Element.
Proteinase activity detects：Bacillus coagulans is inoculated in 10ml LB liquid mediums, 37 DEG C of culture 48h, 8000
× g takes supernatant after centrifuging 20 minutes, and uses 0.22 μm of filtering with microporous membrane, takes the addition of 200 μ l filtrates to be placed on casein
In the Oxford cup of flat board (25g/L skimmed milk powers, 15g/L agar, 1000ml distilled water), 37 DEG C of culture 48h, transparent circle is detected
Occur.There is obvious transparent circle in periphery of bacterial colonies after culture, illustrates that the bacterial strain generates protease, by the casein in culture medium
Degraded.
Gemma sprout time：Gemma can be sprouted under optimum conditions to work for trophosome, gemma in animal intestinal tract
It is trophosome to need to sprout, therefore the sprout time and rule of gemma can be understood by detecting the quantity of gemma.Bud will be condensed
Spore bacillus CGMCC NO.10221 are inoculated in LB fluid nutrient mediums in LB agar lining out overnight incubations, picking single bacterium colony, training
80 DEG C of water-bath 15min after foster 48h, after the zymotic fluid after water-bath is cooked into 10 times of gradient dilutions, choose suitable dilution factor and are coated on
LB agar plates, calculate the quantity of gemma.Gemma liquid 1ml after water intaking bath is inoculated in 9ml LB liquid mediums, and 30 DEG C are cultivated 4h,
Sampled respectively at 2h and 4h, detect gemma and number of viable.Under suitable conditions, the Bacillus coagulans spore is sprouted in 2h
For trophosome, therefore quickly can be played a role in animal intestinal tract.
Antibiotics sensitivity：Bacillus coagulans CGMCC NO.10221 is lined into LB agar plates, 30 DEG C are incubated overnight,
Picking single bacterium colony is inoculated in LB liquid medium, and 30 DEG C, 150rpm is incubated overnight, and nutrient solution is diluted into 106Cfu/ml, draw
10 μm are added in the LB soft agar mediums of 10ml dissolvings, are tiled after mixing onto LB agar plates, and difference will be contained after cooling
The susceptibility piece of antibiotic concentration is positioned on agar plate, and inhibition zone is observed after 30 DEG C of overnight incubations.As a result show, the bacterial strain is to Ji
Its mycin is sensitive
Embodiment 1
A kind of layer dietses of rich omega-3 polyunsaturated fatty acids egg, prepared by the raw material of following parts by weight：
20 parts of barley, 40 parts of corn, 5 parts of fermented bean dregs, 5 parts of corn protein powder, 2 parts of brewer's yeast, 5 parts of expanded soybean,
Expanded 5 parts of linseed, 6 parts of oat bran, 3 parts of Alfalfa, 5 parts of super-micro wall-broken grape pip, 2 parts of fish oil, 3 parts of fish meal, marine alga
8 parts of powder, 8 parts of stone flour, 0.5 part of calcium monohydrogen phosphate, 0.2 part of salt, 0.35 part of amino acid, 0.5 part of compound premix, yeast selenium
0.005 part, 0.02 part of vitamin E, 0.05 part of vitamin C, 0.05 part of complex enzyme formulation, 0.05 part of Choline Chloride, glycine betaine
0.08 part, 0.05 part of probiotic, it is 0.3 part to win kind X-1,0.01 part of phytase, 0.05 part of lipase.
The amino acid is lysine, methionine, threonine and tryptophan in mass ratio 1：1.5:0.5:0.1 is uniformly mixed
Close.
The probiotic is mannosan, Fructooligosaccharides, zymosan, bacillus coagulans, bacillus licheniformis, yeast
At least one of bacterium, lactic acid bacteria；
It is 3 that the probiotic, which includes parts by weight,:1.5:2.3 mannosan, Fructooligosaccharides, zymosan, at the same it is described
Living bacteria count in probiotic is：Bacillus coagulans >=11,000,000,000/g, bacillus licheniformis >=100,000,000,000/g, saccharomycete >=
12000000000/g, lactic acid bacteria >=6,000,000,000/g；
The bacillus coagulans is the bacillus coagulans with antibacterial activity, is separated from health-care eggs chicken intestinal
To a bacillus, its zymotic fluid has good antibacterial activity to C.perfringens, is reflected through Physiology and biochemistry and genotype
Fixed, the bacterial strain is bacillus coagulans, is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, preservation
Numbering is CGMCC NO.10221.
The ultramicro grinding grape pip disintegrating process is to crush grape pip through 200 mesh, with bentonite, wheat bran with 90:
2:8 ratio carries out low temperature granulation.
A kind of preparation method of the layer dietses of rich omega-fatty acid egg, comprises the following steps：
Each component raw material accurately is weighed according to formula, is crushed according to conventional egg feedstuff processing method, then by weight
Number is arrogant to small to sequentially add various raw materials, is well mixed, processing, produces one kind and is rich in omega-3 polyunsaturated fatty acids egg
Layer dietses.
Embodiment 2
A kind of layer dietses of rich omega-3 polyunsaturated fatty acids egg, are prepared from the following raw materials in parts by weight：
10 parts of barley, 30 parts of corn, 10 parts of fermented bean dregs, 10 parts of corn protein powder, expanded 2 parts of linseed, brewer's yeast 2
Part, 10 parts of expanded soybean, 3 parts of oat bran, 6 parts of Alfalfa, 2 parts of super-micro wall-broken grape pip, 4 parts of fish oil, 1 part of fish meal, sea
2 parts of algae powder, 10 parts of stone flour, 0.8 part of calcium monohydrogen phosphate, 0.4 part of salt, 0.53 part of amino acid, 0.5 part of compound premix, yeast selenium
0.003 part, 0.01 part of vitamin E, 0.05 part of vitamin C, 0.1 part of complex enzyme formulation, 0.12 part of Choline Chloride, glycine betaine
0.03 part, 0.1 part of probiotic, it is 0.3 part to win kind rich element X-1,0.03 part of phytase, 0.03 part of lipase.
The amino acid is lysine, methionine, threonine and tryptophan in mass ratio 1：0.7:0.5:0.1 is uniformly mixed
Close.
It is 6 that the probiotic, which includes parts by weight,:3.5:5.5 mannosan, Fructooligosaccharides, zymosan, at the same it is described
Living bacteria count in probiotic is：Bacillus coagulans >=11,000,000,000/g, bacillus licheniformis >=100,000,000,000/g, saccharomycete >=
12000000000/g, lactic acid bacteria >=6,000,000,000/g；
The bacillus coagulans is the bacillus coagulans with antibacterial activity, deposit number CGMCC
NO.10221。
The ultramicro grinding grape pip disintegrating process is to crush grape pip through 200 mesh, with bentonite, wheat bran with 90:
2:8 ratio carries out low temperature granulation.
A kind of preparation method of the layer dietses of rich omega-fatty acid egg, comprises the following steps：
Each component raw material accurately is weighed according to formula, is crushed according to conventional egg feedstuff processing method, then by weight
Number is arrogant to small to sequentially add various raw materials, is well mixed, processing, produces one kind and is rich in omega-3 polyunsaturated fatty acids egg
Layer dietses.
Embodiment 3
A kind of layer dietses of rich omega-3 polyunsaturated fatty acids egg, are prepared from the following raw materials in parts by weight：
15 parts of barley, 35.6 parts of corn, 6 parts of fermented bean dregs, 8 parts of corn protein powder, 3.5 parts of brewer's yeast, expanded soybean
6.3 parts, expanded 3.7 parts of linseed, 6 parts of oat bran, 4.2 parts of Alfalfa, 3.6 parts of super-micro wall-broken grape pip, 3 parts of fish oil,
2 parts of fish meal, 5 parts of seawood meal, 9 parts of stone flour, 0.7 part of calcium monohydrogen phosphate, 0.3 part of salt, 0.5 part of amino acid, compound premix 0.5
Part, 0.004 part of yeast selenium, 0.02 part of vitamin E, 0.03 part of vitamin C, 0.05 part of complex enzyme formulation, Choline Chloride 0.08
Part, 0.06 part of glycine betaine, 0.1 part of probiotic, it is 0.3 part to win kind rich element X-1,0.03 part of phytase, 0.1 part of lipase.
The amino acid is lysine, methionine, threonine and tryptophan in mass ratio 1：0.7:1.0:0.1 is uniformly mixed
Close.
It is 4 that the probiotic, which includes parts by weight,:2.6:4 mannosan, Fructooligosaccharides, zymosan, while the benefit
Living bacteria count in raw element is：Bacillus coagulans >=11,000,000,000/g, bacillus licheniformis >=100,000,000,000/g, saccharomycete >=120
Hundred million/g, lactic acid bacteria >=6,000,000,000/g；
The bacillus coagulans is the bacillus coagulans with antibacterial activity, and preserving number is CGMCC NO.10221.
Other are the same as embodiment 1.
A kind of preparation method of the layer dietses of rich omega-fatty acid egg, with embodiment 1.
Embodiment 4
A kind of layer dietses of rich omega-3 polyunsaturated fatty acids egg, are prepared from the following raw materials in parts by weight：
12 parts of barley, 36 parts of corn, 8 parts of fermented bean dregs, 8 parts of corn protein powder, 3 parts of brewer's yeast, 7 parts of expanded soybean,
Expanded 5 parts of linseed, 4 parts of oat bran, 5 parts of Alfalfa, 5 parts of super-micro wall-broken grape pip, 3 parts of fish oil, 3 parts of fish meal, marine alga
6 parts of powder, 9.5 parts of stone flour, 0.7 part of calcium monohydrogen phosphate, 0.2 part of salt, 0.7 part of amino acid, 0.5 part of compound premix, yeast selenium
0.005 part, 0.02 part of vitamin E, 0.05 part of vitamin C, 0.05 part of complex enzyme formulation, 0.05 part of Choline Chloride, glycine betaine
0.08 part, 0.1 part of probiotic, it is 0.3 part to win kind rich element X-1,0.03 part of phytase, 0.04 part of lipase.
The amino acid is lysine, methionine, threonine and tryptophan in mass ratio 1：0.7:0.5:0.1 is uniformly mixed
Close.
It is 6 that the probiotic, which includes parts by weight,:1.5:2.3 mannosan, Fructooligosaccharides, zymosan；
Other are the same as embodiment 1.
A kind of preparation method of the layer dietses of rich omega-fatty acid egg, with embodiment 1.
Embodiment 5
A kind of layer dietses of rich omega-3 polyunsaturated fatty acids egg, are prepared from the following raw materials in parts by weight：Barley
20 parts, 30 parts of corn, 5 parts of fermented bean dregs, 5 parts of corn protein powder, 3.8 parts of brewer's yeast, 10 parts of expanded soybean, expanded linseed
4.6 parts, 4.8 parts of oat bran, 4.5 parts of Alfalfa, 3.6 parts of super-micro wall-broken grape pip, 4 parts of fish oil, 1 part of fish meal, seawood meal 6
Part, 10 parts of stone flour, 0.8 part of calcium monohydrogen phosphate, 0.3 part of salt, 0.6 part of amino acid, 0.5 part of compound premix, yeast selenium 0.005
Part, 0.02 part of vitamin E, 0.02 part of vitamin C, 0.05 part of complex enzyme formulation, 0.05 part of Choline Chloride, 0.08 part of glycine betaine,
0.05 part of probiotic, it is 0.3 part to win kind rich element X-1,0.03 part of phytase, 0.08 part of lipase.
The amino acid is lysine, methionine, threonine and tryptophan in mass ratio 1：1.5:1.0:0.5 is uniformly mixed
Close.
Living bacteria count in the probiotic is：Bacillus coagulans >=11,000,000,000/g, bacillus licheniformis >=1000
Hundred million/g, saccharomycete >=12,000,000,000/g, lactic acid bacteria >=6,000,000,000/g；
The bacillus coagulans is the bacillus coagulans with antibacterial activity, and preserving number is CGMCC NO.10221.
Other are the same as embodiment 1.
A kind of preparation method of the layer dietses of rich omega-fatty acid egg, with embodiment 1.
In embodiment 1-5
The compound premix：Liaoning Hefeng Animal Husbandry Co., Ltd produces, Q/HF J02.03-2012, product approval
Code raises pre- word (2013) 003024 for the Liao Dynasty；Product form is B B-complex and trace element, and trade mark is that standing grain is rich, and market has
Sell.Compound premix composition is as follows：Iron 15000mg/Kg, copper 3000mg/Kg, zinc 20000mg/Kg, manganese 24000mg/Kg, selenium
60mg/Kg, iodine 100mg/Kg, retinol1 600KIU, vitamin D 600KIU, vitamin E 320mg/Kg, vitamin
K3200mg/Kg, vitamin B1200mg/Kg, vitamin B2880mg/Kg, vitamin B6600mg/Kg, vitamin B122.0mg/
Kg, nicotinic acid 6000mg/Kg, pantothenic acid 800mg/Kg, folic acid 100.0mg/Kg, biotin 5.5mg/Kg；
The phytase：10,000 unit phytases of Beijing Smistyle Science ＆ Technology Development Co., Ltd.'s production, production licence number
Add (2012) 0017 to raise, authentication code：Raise (adding) word (2012) 403711 in capital；Main component is phytase；It is commercially available；
The complex enzyme formulation：Shenyang Fengmei Biotechnology Co., Ltd. produces, and production licence number is that feeding adds (2011)
1986, authentication code：(adding) word (2011) 040008 is raised by the Liao Dynasty；Contents of Main Components is as follows：Zytase 3000U/g, sweet dew gather
Carbohydrase 800U/g, acid protease U/g, lipase 800U/g, amylase 300U/g, dextranase 400U/g.It is commercially available；
Test example, done with a kind of layer dietses of rich omega-3 polyunsaturated fatty acids egg of the present invention with commercially available similar feed
Check experiment, more each feed is to DHA content in Layer Production Performance, immunologic function, anti-oxidation function, egg and egg ω -3
Aliphatic acid, and done shelf life experiment.
1. test material and method
The 1.1 21 days-Septembers 21 days of test period 2 months 2016, phase experimental period each tested are 60 days, have carried out 20
15 average values tested, are now summarized as follows by the laying hen experiment of batch.
1.2 test site：The clear fowl industry cultivation Co., Ltds of Fuyu City Fuyu County of Jilin Province standing grain Feng Taolai.
1.3 experimental design：Test chicken use 1152 Beijing exit of valley company sea blue brown laying hen, test chicken be 20 week old-
50 week old, it is divided into totally 6 groups of present invention group embodiment 1-5 and control group, every group of 6 repetitions, each repeats 96 chickens, implement number of cases
According to averaging, contrasted with control group；
1.4 feeding method：Manual feeding, three times a day, free choice feeding；Free water, water are running water, water temperature 15-21
℃。
1.5 hen house：Longitudinal ventilation, hen house temperature are 20 DEG C -28 DEG C.According to the sea blue brown layer breeding management of exit of valley company
Program strict implement controls.
1.6 Testing index
1.6.1 egg laying performance index：Initially with terminating body weight, egg production, lay eggs a piece number, egg size, feed intake and feedstuff-egg ratio；
1.6.2 immune function in blood：IgG,IgA,IgM.Blood-sampling method uses Culling heart blood.
1.6.3 Antioxidant Indexes in blood：TAC T-AOC, glutathione peroxidase GSH-PX (U/
Ml),
Superoxide dismutase T-SOD (U/ml), MDA MDA (umol/L).
1.6.4 Egg Quality testing result, using egg product detector
1.6.5 fatty acid value detection (19 kinds) in egg, is analyzed using 7890B gas chromatographs.
2. result of the test：Testing result statistics will be carried out per batch experiment index, and calculate each batch average, statistical result
Such as table 5, table 6 and table 7.
Table 1 is to the week old synthesis result contrast table of laying hen 20-50
| Project | Of the present invention group | Control group |
| Original body mass (kilogram) | 1.65±0.83 | 1.67±0.61 |
| Terminate body weight (kilogram) | 1.99±0.62 | 1.91±0.95 |
| Average egg weight (gram) | 62.8±0.06a | 61.2±0.09b |
| Laying rate (%) | 92.3±0.55a | 90.1±0.76b |
| Egg production (gram/only/day) | 57.96±0.49a | 55.1±0.84b |
| Profit (member/only) | 130.8 | 20.2 |
From table 1, present invention group laying rate improves 2.44%, and egg production improves 5.19%, and egg laying performance significantly improves.
Rich DHA eggs calculate according to yuan/piece of wholesale price 1.5, and common egg calculates according to 6.4 yuan/kilogram.The feed of rich DHA eggs
Cost reduces by 56.8% than the DHA egg feeds cost in market.
Table 2 is to laying hen immunologic function and the influence table of comparisons of anti-oxidation function
From table 2, laying hen of the present invention significantly improves in 20-50 week old stages immunologic function and anti-oxidation function.
The influence table of comparisons of the table 3 to the week old Egg Quality of laying hen 20-50
| Project | Of the present invention group | Control group |
| Hough unit | 90.60±2.12 | 87.7±3.74 |
| White of an egg height | 8.11±0.68 | 7.95±1.16 |
| Yolk ratio | 29.32±1.55 | 26.43±2.29 |
| Eggshell strength | 4.928±0.49a | 4.264±0.83b |
| Shell thickness | 0.405±0.05 | 0.383±0.07 |
| Commodity egg rate | 93.5±2.58 | 90.1±3.79 |
From table 3, in the laying hen 20-50 week old stages, eggshell strength and Hough unit present invention group effect are fairly obvious,
Hough unit improves 3.31%, illustrates that the freshness of egg is high.Eggshell strength improves 15.57%, and shell thickness improves 5.74%,
3.87% is improved by the qualified egg rate of strict picking commodities.It has also been found that, the egg of test group, shell membrane thickness is significantly larger than in experiment
Control group, accurately determined with regard to no because inconvenience detects.
DHA content and the egg omega-fatty acid table of comparisons (unit in the egg product of table 4：Milligram/100 grams of eggs, day)
| Project | Of the present invention group | Control group |
| DHA | 438.8±3.23 | 123.7±5.76 |
| Omega-3 polyunsaturated fatty acids | 586.5±0.68 | 128.2±1.12 |
| Omega 6 polyunsaturated fatty acid | 1083.9±6.71 | 342.4±7.93 |
| Lutein | 23.62±0.27 | 12.35±0.34 |
| Shelf life (my god) | 65.1±0.49a | 40.6±0.83b |
The data of table 4 are the testing results of Beijing inspection center of nutrient source research institute, and DHA content is up to 438.8 millis in egg
Gram/100 grams of eggs, in industry reach peak, illustrate that significantly improving fat using lipase and special probiotics disappears
Change absorptivity, improve DHA in egg accumulation ability；586.5 milligrams/100 grams of omega-3 polyunsaturated fatty acids simultaneously, than
Control group improves 357.5%；1083.9 milligrams/100 grams of omega 6 polyunsaturated fatty acid, 216.6% is improved than control group；
Lutein content improves 91.25%.Take aggregate measures to enhance the oxidation resistance of DHA eggs, significantly by shelf life from
Extend within 40.6 days 65.1 days.
Claims (10)
1. a kind of layer dietses of rich omega-3 polyunsaturated fatty acids egg, are prepared from the following raw materials in parts by weight：Barley 10-
20 parts, corn 30-40 parts, fermented bean dregs 5-10 parts, corn protein powder 5-10 parts, brewer's yeast 2-4 parts, expanded soybean 5-10
Part, expanded linseed 2-5 parts, oat bran 3-6 parts, Alfalfa 3-6 parts, super-micro wall-broken grape pip 2-5 parts, fish oil 2-4 parts,
Fish meal 1-3 parts, seawood meal 2-8 parts, stone flour 8-10 parts, calcium monohydrogen phosphate 0.5-0.8 parts, salt 0.2-0.4 parts, amino acid 0.35-
1.0 parts, 0.5 part of compound premix, yeast selenium 0.003-0.005 parts, vitamin E 0.01-0.02 parts, vitamin C 0.02-
0.05 part, complex enzyme formulation 0.05-0.1 parts, Choline Chloride 0.05-0.12 parts, glycine betaine 0.03-0.08 parts, probiotic 0.05-
0.1 part, it is 0.3 part to win kind rich element X-1, phytase 0.01-0.03 parts, lipase 0.03-0.1 parts.
2. the layer dietses of rich omega-3 polyunsaturated fatty acids egg according to claim 1, it is characterised in that the amino
Acid is lysine, methionine, threonine and tryptophan in mass ratio 1：0.7-1.5:0.5-1.0:0.1-0.5 is uniformly mixed.
3. the layer dietses of rich omega-3 polyunsaturated fatty acids egg according to claim 1, it is characterised in that described compound
Premix is：Liaoning Hefeng Animal Husbandry Co., Ltd produces, and product authentication code raises pre- word (2013) 003024 for the Liao Dynasty.
4. the layer dietses of rich omega-3 polyunsaturated fatty acids egg according to claim 1, it is characterised in that the phytic acid
Enzyme is：10,000 unit phytases of Beijing Smistyle Science ＆ Technology Development Co., Ltd.'s production, authentication code：Raise (adding) word (2012) in capital
403711。
5. the layer dietses of rich omega-3 polyunsaturated fatty acids egg according to claim 1, it is characterised in that described prebiotic
Element is mannosan, Fructooligosaccharides, zymosan, bacillus coagulans, bacillus licheniformis, saccharomycete, in lactic acid bacteria at least
It is a kind of.
6. the layer dietses of rich omega-3 polyunsaturated fatty acids egg according to claim 5, it is characterised in that described prebiotic
Element is 3-6 comprising parts by weight:1.5-3.5:2.3-5.5 mannosan, Fructooligosaccharides, zymosan.
7. the layer dietses of rich omega-3 polyunsaturated fatty acids egg according to claim 6, it is characterised in that described prebiotic
Living bacteria count in element is：Bacillus coagulans >=11,000,000,000/g, bacillus licheniformis >=100,000,000,000/g, saccharomycete >=120
Hundred million/g, lactic acid bacteria >=6,000,000,000/g.
8. the layer dietses of rich omega-3 polyunsaturated fatty acids egg according to claim 7, it is characterised in that the condensation
Bacillus is preservation of bacteria strain, and deposit number is CGMCC No.10221.
9. the layer dietses of rich omega-3 polyunsaturated fatty acids egg according to claim 1, it is characterised in that the ultra micro
Broken wall grape pip is prepared by the following method：Grape pip is crushed through 200 mesh, with bentonite, wheat bran with 90:2:8 ratio is entered
Row low temperature is pelletized.
10. the method for the layer dietses of any rich omega-3 polyunsaturated fatty acids eggs of claim 1-9 is prepared, including such as
Lower step：
Each component raw material accurately is weighed according to formula, is crushed according to conventional egg feedstuff processing method, then in parts by weight
It is arrogant to small to sequentially add various raw materials, it is well mixed, processing, produces the laying hen day of rich omega-3 polyunsaturated fatty acids egg
Grain.
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Cited By (8)
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| CN107927429A (en) * | 2017-12-29 | 2018-04-20 | 新津邦得科技有限公司 | A kind of egg feedstuff |
| CN108850659A (en) * | 2018-09-29 | 2018-11-23 | 辽宁禾丰牧业股份有限公司 | A kind of laying cycle of laying hens feed and preparation method thereof extending egg shelf life |
| CN109123180A (en) * | 2018-08-14 | 2019-01-04 | 天津农学院 | Improve the feed formula for laying hen of omega-3 polyunsaturated fatty acids content in egg |
| CN109156602A (en) * | 2018-09-29 | 2019-01-08 | 辽宁禾丰牧业股份有限公司 | A kind of laying hen compound premixed feed and preparation method thereof extending egg shelf life |
| CN112167165A (en) * | 2020-09-29 | 2021-01-05 | 厦门汇盛生物有限公司 | Preparation and application of compound rumen-bypass polyunsaturated fatty acid powder |
| CN112369531A (en) * | 2020-12-03 | 2021-02-19 | 湖南省畜牧兽医研究所 | Feed additive for reducing fresh egg yolk-dispersing rate |
| CN112876350A (en) * | 2021-02-18 | 2021-06-01 | 淳安天龙生物科技有限公司 | Process for preparing plant water-soluble omega 3 from flaxseeds |
| CN114698747A (en) * | 2022-04-20 | 2022-07-05 | 山东和美华农牧科技股份有限公司 | Laying hen feed additive for improving yolk cholesterol content and production method of low-cholesterol eggs |
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| CN109123180A (en) * | 2018-08-14 | 2019-01-04 | 天津农学院 | Improve the feed formula for laying hen of omega-3 polyunsaturated fatty acids content in egg |
| CN108850659A (en) * | 2018-09-29 | 2018-11-23 | 辽宁禾丰牧业股份有限公司 | A kind of laying cycle of laying hens feed and preparation method thereof extending egg shelf life |
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| CN112167165B (en) * | 2020-09-29 | 2022-02-11 | 厦门汇盛生物有限公司 | Preparation and application of compound rumen-bypass polyunsaturated fatty acid powder |
| CN112369531A (en) * | 2020-12-03 | 2021-02-19 | 湖南省畜牧兽医研究所 | Feed additive for reducing fresh egg yolk-dispersing rate |
| CN112876350A (en) * | 2021-02-18 | 2021-06-01 | 淳安天龙生物科技有限公司 | Process for preparing plant water-soluble omega 3 from flaxseeds |
| CN114698747A (en) * | 2022-04-20 | 2022-07-05 | 山东和美华农牧科技股份有限公司 | Laying hen feed additive for improving yolk cholesterol content and production method of low-cholesterol eggs |
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