CN107475341A - A kind of preparation method of extra large intestines active peptide - Google Patents
A kind of preparation method of extra large intestines active peptide Download PDFInfo
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- CN107475341A CN107475341A CN201710927954.0A CN201710927954A CN107475341A CN 107475341 A CN107475341 A CN 107475341A CN 201710927954 A CN201710927954 A CN 201710927954A CN 107475341 A CN107475341 A CN 107475341A
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Abstract
A kind of preparation method of extra large intestines active peptide.The present invention relates to traditional Chinese medicine and technical field of biology.It is formed using fresh extra large intestines as raw material through broken homogenate, enzymolysis, centrifugation, sterilizing, ultrafiltration.Extra large intestines after homogenate are adjusted into pH value 68, add the proteolytic enzyme of extra large intestines weight 0.1 1%, are digested 36 hours at 50 60 degrees Celsius；Add the dusty yeast of extra large intestines weight 0.5% 1% defishying 12 hours between 30 50 degree Celsius；Then enzyme deactivation, centrifugation, sterilizing, ultrafiltration obtain the extra large intestines active peptide liquid below the dalton of molecular weight 10000；Freeze-dried powder product, oral liquid product, capsule product can be prepared.Described proteolytic enzyme is combination more than one or both of neutral proteinase, trypsase, compound protease, flavor protease, papain.The extra large intestines active peptide has the pharmacological activity such as warm filling liver kidney, establishing-Yang controlling nocturnal emission with astringent drugs, antitumor, antithrombotic, strengthen immunity, beautifying face and moistering lotion.Process scientific and precise of the present invention, strong operability, is easy to industrialized production.
Description
Technical field
The present invention relates to traditional Chinese medicine and technical field of biology, more particularly to one kind to have warm filling liver kidney, establishing-Yang controlling nocturnal emission with astringent drugs, resist and swell
Knurl, antithrombotic, strengthen immunity, effects of beautification and nourishing face extra large intestines active peptide preparation method.
Background technology
Extra large intestines, scientific name Urechis uniconctus (Urechis unicinctus), popular name:Extra large intestines, extra large intestines, belong to echiuran
Door (Echiurioidea), Yi guiding principles (Echiurida), without pipe Yi mesh (Xenopneusta), thorn Yi sections (Urchidae).It is
A kind of mollusk of oval tubular, wriggles, from head to foot impulse- free robustness softly, light yellow.Extra large intestines not only look like nude sea cucumber,
Its nutritive value is also in no way inferior compared with sea cucumber.Extra large intestines have stronger tolerance and detoxification ability to sulfide, are also therefore taken as edge
The model organism of extra large sulfide metabolism correlative study.Extra large intestines are mainly distributed within the border in Shandong:The coastal mud in the ground such as Yantai, Qingdao
In beach or rock seam.Shandong Jiaodong District is the maximum place of production of China sea intestines.Its individual is loose, and meat flavour is delicious, and body wall flesh is rich in
Protein and a variety of essential amino acids.Since ancient times, it is coastal as rare seafood food in China, Japan and Korea
Product, there is higher economic value.The traditional method of people only eats its body wall, and discarded internal organ, dish are commonly called as extra large intestines.But have
Research finds that it is 18.25% that extra large intestines, which discard visceral protein matter content, fat content 0.12%, total sugar content 4.09%, and
Contain the elements such as abundant Ca, Mg, Fe, Zn；Also containing abundant EPA, DHA and DPA.Pharmaceutical research shows：Extra large intestines（It is single
Ring pierces Yi）The polypeptide of extraction plays the role of certain antitumor and raising immune function of mice.Also someone did extra large intestines（Monocyclic thorn
Yi）Antithrombotic component isolate and purify and pharmacodynamic study.Therefore extra large intestines（Urechis uniconctus）And a kind of research marine organisms live
The good material of property material.Extra large intestines also known as " extra large chicken ", are produced in the fisherman of northern Shandong in Chinese only Bohai Sea Gulf, softly compacted
Dynamic, impulse- free robustness, light yellow from head to foot.It is called " nude sea cucumber " in some local people, extra large intestines not only look like nude sea cucumber,
Its nutritive value is also in no way inferior compared with sea cucumber.But for many years, people use it as " bait ".Really it is used for making
The history of cook dish only decades.Population can be eaten, and had the function that warm filling liver kidney, establishing-Yang controlling nocturnal emission with astringent drugs, be particularly suitable for
Male eats.
Active peptide is the general name of more than 1,000 kinds of peptide.It grows people's, metabolism, disease and aging, dead
During play key effect.Active peptide is most important active material in human body.Exactly because its secretory volume in vivo
Increase or reduce, the mankind is had childhood, in childhood, adult is old until the cycle of death.And take active peptide and just break
This cycle of life, so as to reach extending life, the miraculous effects of effective delaying senility.Peptide in vivo as carrier and
Means of transport, by the nutrient delivery that people are eaten usually to each position of human body, give full play to its function；Peptide is made in vivo
For neurotransmitter, transmission information；Peptide has extremely strong activity and diversity, fully adjustable human body physiological function, strengthens human body
Physiologically active；Peptide can not only provide the nutriment needed for growth in humans's development, and have special biological function, can prevent
Control thrombus, high fat of blood, hypertension, anti-aging is antifatigue, improve immunity of organisms, promote human body to protein, vitamin,
A variety of absorptions to human body healthy trace elements with household such as amino acid, calcium, iron, zinc, selenium, magnesium, copper；Some small peptides have former food protein
Matter or other composition unexistent important physiological functions of amino acid.The less small peptide of some molecular weight in biologically active peptide, with
Amino acid transport function phase ratio, the small peptide in biologically active peptide, which has, absorbs quick, low consumption and undersaturated feature.
Extra large gut function is no doubt a lot, but because molecular weight of albumen is bigger, so general eating method is digested and assimilated and compared
It is few.Present extra large intestines do not have deep processed product also, are merely possible to dish raw material and are sold in seafood market, there is the wasting of resources for this
The health-care effect of extra large intestines can not be more given full play to simultaneously.Therefore how will there is warm filling liver kidney, establishing-Yang controlling nocturnal emission with astringent drugs, antitumor, anti-
The extra large intestines of the pharmacological activity such as thrombus, strengthen immunity, effects of beautification and nourishing face make function and absorb more preferable active peptide, preferably
Reach the purpose that traditional Chinese medicine preventives treatment of disease, really realize the modernization of Chinese medicine, it has also become traditional Chinese medicine and technical field of biology are urgently to be resolved hurrily
Problem.
The content of the invention
In order to overcome wastage of material and absorption of human body utilization rate in extra large intestines active material extraction process in the prior art
Low deficiency, the invention provides a kind of preparation method of live body sea intestines active peptide, biology is rich in the live body sea intestines active peptide
Active peptide, there is the pharmacological activity such as warm filling liver kidney, establishing-Yang controlling nocturnal emission with astringent drugs, antitumor, antithrombotic, strengthen immunity, beautifying face and moistering lotion, and
It is easy to digest and assimilate, nutritious body utilization rate is high.The preparation method technique of the live body sea intestines active peptide is rigorous rationally, can grasp
The property made is strong, it is easy to accomplish industrialized production.
The purpose of the present invention is achieved through the following technical solutions：A kind of preparation method of extra large intestines active peptide, its is special
Part is：
1st, choosing fresh and alive extra large intestines goes internal organ to clean up with the obtained live body sea intestines homogenate of historrhexis's crusher machine；The extra large intestines of taking-up
Internal organ removal silt cleans up is made the dirty homogenate of live body sea enteral with historrhexis's crusher machine,
2nd, by the live body of above-mentioned preparation, pH value 6-8 is adjusted in extra large intestines homogenate entirely, adds the 0.1-1% of live body sea intestines weight proteolysis
Enzyme, after digesting 4-8 hours under temperature conditionss between 45-60 degrees Celsius of temperature, add live body sea intestines weight 0.5%-1% yeast
Powder stirs, and temperature control carries out defishying between 30-50 Celsius, after 1-2 hours by temperature be adjusted to 85 degree Celsius with
Upper water-bath 5-15 minutes carry out enzyme deactivation, and live body extra large intestines enzymolysis liquid entirely is made；
3rd, the extra large intestines enzymolysis liquid of above-mentioned preparation is subjected to melt cinder separation with supercentrifuge, centrifugal speed is adjusted to 9500-14000 and turned
Between, extra large intestines centrifugation enzymolysis liquid is made；
4th, the extra large intestines centrifugation enzymolysis liquid of above-mentioned preparation is put into stainless steel cask and enters steam sterilization cabinet steam between 100-120 degree Celsius
Autoclaving 15-40 minutes；
5th, the extra large intestines of above-mentioned preparation centrifugation enzymolysis liquid is filtered into obtain sea below the dalton of molecular weight 10000 by ultrafiltration membrane filter
Intestines active peptide liquid；
It is 6th, the extra large intestines active peptide liquid of above-mentioned preparation is filling to freeze dryer vacuum freeze drying on cillin bottle by lyophilized technique specification,
Produce the extra large bottled freeze-dried powder finished product of intestines active peptide of the present invention；Pure water is added to be given birth to by oral liquid the extra large intestines active peptide liquid of above-mentioned preparation
Production. art specification is filling to oral liquid bottle, produces the extra large intestines oral liquid of active peptide finished product of the present invention；By the extra large intestines activity of above-mentioned preparation
Peptide liquid is freeze-dried or spray drying is prepared into powdery granule, canned into capsule by capsule manufacturing techniques specification, produces this
Invent extra large intestines active peptide capsule finished product.
Proteolytic enzyme of the present invention is neutral proteinase, trypsase, compound protease, flavor protease, wood
Combination more than one or both of melon protease.
Extra large intestines are raw material to the present invention entirely from live body, and active peptide solution is generated by controlled enzymatic hydrolysis, through enzyme deactivation, deodorant, from
It is filling filling into capsule formulation and bottled freeze dried powder into oral liquid formulation, spray drying or freeze-drying after the heart, sterilizing, ultrafiltration
Type, its nutritive value and absorption rate and edible safety greatly improve, and have warm filling liver kidney, establishing-Yang controlling nocturnal emission with astringent drugs, resist and swell
The pharmacological activity such as knurl, antithrombotic, strengthen immunity, beautifying face and moistering lotion, the preparation method technique of the extra large intestines active peptide is rigorous rationally, can
Strong operability, it is easy to accomplish industrialized production.
Embodiment
With reference to embodiment, the present invention will be further described
Embodiment one, 1, the preparation of extra large intestines active peptide liquid：Choosing fresh and alive extra large intestines 100kg goes internal organ to clean up with historrhexis's machine
It is broken that extra large intestines homogenate is made；The dirty silt that removes of the extra large enteral of taking-up is cleaned up that extra large enteral is made with historrhexis's crusher machine is dirty
Homogenate,
2nd, by step 1, the live body prepared, PH7 is adjusted in extra large intestines homogenate entirely, is added trypsase, papain, flavor protease and is entered
Row multienzyme digests, and enzyme ratio of components is 1：1：1, enzyme dosage is that every gram of live body sea intestines add 6000 enzyme-activity units (u), temperature control
The dusty yeast that live body sea intestines weight 0.8% is added after being digested 6 hours at 55 degree Celsius stirs, and temperature control is at 35 degree Celsius
Defishying is carried out, after 1.5 hours boiling water heating bath enzyme deactivation 15 minutes, produce extra large intestines enzymolysis liquid；
3rd, step 2, the extra large intestines enzymolysis liquid handled well are subjected to melt cinder separation with supercentrifuge, centrifugal speed is adjusted to 12000rpm
Left and right, the extra large intestines enzymolysis liquid of centrifugation is made；
4th, step 3, the extra large intestines enzymolysis liquid prepared are put stainless steel cask and enters steam sterilization cabinet in 105 degree of steam high-voltage sterilizings 35 Celsius
Minute；
5th, step 4, the extra large intestines enzymolysis liquid prepared are filtered into obtain extra large intestines below the dalton of molecular weight 10000 by ultrafiltration membrane filter
Active peptide liquid；
6th, step 5, the extra large intestines active peptide liquid prepared are done to freeze dryer vacuum refrigeration on cillin bottle by lyophilized technique specification is filling
It is dry, produce the extra large bottled freeze-dried powder finished product of intestines active peptide of the present invention；Extra large intestines active peptide liquid prepared by step 5 adds pure water by oral
Liquid production technology specification is filling to oral liquid bottle, produces the extra large intestines oral liquid of active peptide finished product of the present invention；Extra large intestines prepared by step 5
Active peptide liquid is freeze-dried or spray drying is prepared into powdery granule, canned into capsule by capsule manufacturing techniques specification, i.e.,
Obtain extra large intestines active peptide capsule finished product of the invention.
Embodiment two, 1, the preparation of extra large intestines active peptide liquid：Choose fresh and alive extra large intestines 150kg and go internal organ to clean up and use tissue
The homogenate of live body sea intestines is made in crusher in crushing；The dirty silt that removes of extra large enteral of taking-up is cleaned up with historrhexis's crusher machine system
The extra large dirty homogenate of enteral is obtained,
2nd, PH6.5 is adjusted in extra large intestines homogenate to the live body for preparing step 1 entirely, adds neutral proteinase, papain, flavor protease
Multienzyme enzymolysis is carried out, enzyme ratio of components is 1：1：1, enzyme dosage is that every gram of live body sea intestines add 10000 enzyme-activity units (u), temperature
The dusty yeast for adding live body sea intestines weight 0.9% after being digested 6 hours at 55 degree Celsius is controlled to stir, temperature control is Celsius
38 degree of progress defishyings, after 2 hours boiling water heating bath enzyme deactivation 15 minutes, produce extra large intestines enzymolysis liquid；
3rd, the extra large intestines enzymolysis liquid for handling step 2 well carries out melt cinder separation with supercentrifuge, and centrifugal speed is adjusted to 13000rpm
Left and right, the extra large intestines enzymolysis liquid of centrifugation is made；
4th, the extra large intestines enzymolysis liquid for preparing step 3 puts stainless steel cask and enters steam sterilization cabinet in 110 degree of steam high-voltage sterilizings 35 Celsius
Minute；
5th, the extra large intestines enzymolysis liquid for preparing step 4 filters to obtain extra large intestines below the dalton of molecular weight 10000 by ultrafiltration membrane filter
Active peptide liquid；
6th, extra large intestines active peptide liquid prepared by step 5 is done to freeze dryer vacuum refrigeration on cillin bottle by lyophilized technique specification is filling
It is dry, produce the extra large bottled freeze-dried powder finished product of intestines active peptide of the present invention；Extra large intestines active peptide liquid prepared by step 5 adds pure water by oral
Liquid production technology specification is filling to oral liquid bottle, produces the extra large intestines oral liquid of active peptide finished product of the present invention；Extra large intestines prepared by step 5
Active peptide liquid is freeze-dried or spray drying is prepared into powdery granule, canned into capsule by capsule manufacturing techniques specification, i.e.,
Obtain extra large intestines active peptide capsule finished product of the invention.
Embodiment three, 1, the preparation of extra large intestines active peptide liquid：Choose fresh and alive extra large intestines 180kg and go internal organ to clean up and broken with tissue
The homogenate of live body sea intestines is made in broken crusher machine；The dirty removal silt of extra large enteral of taking-up is cleaned up and is made with historrhexis's crusher machine
The dirty homogenate of live body sea enteral,
2nd, PH7.5 is adjusted in extra large intestines homogenate to the live body for preparing step 1 entirely, adds compound protease, papain, flavor protease
Multienzyme enzymolysis is carried out, enzyme ratio of components is 1：1：1, enzyme dosage is that every gram of live body sea intestines add 9000 enzyme-activity units (u), temperature control
The dusty yeast for making addition live body sea intestines weight 1% after being digested 6 hours at 55 degree Celsius stirs, and temperature control is at 35 degree Celsius
Defishying is carried out, after 2 hours boiling water heating bath enzyme deactivation 15 minutes, produce extra large intestines enzymolysis liquid；
3rd, the extra large intestines enzymolysis liquid for handling step 2 well carries out melt cinder separation with supercentrifuge, and centrifugal speed is adjusted to 12000rpm
Left and right, the extra large intestines enzymolysis liquid of centrifugation is made；
4th, the extra large intestines enzymolysis liquid for preparing step 3 puts stainless steel cask and enters steam sterilization cabinet in 120 degree of steam high-voltage sterilizings 30 Celsius
Minute；
5th, the extra large intestines enzymolysis liquid for preparing step 4 filters to obtain extra large intestines below the dalton of molecular weight 10000 by ultrafiltration membrane filter
Active peptide liquid；
6th, extra large intestines active peptide liquid prepared by step 5 is done to freeze dryer vacuum refrigeration on cillin bottle by lyophilized technique specification is filling
It is dry, produce the extra large bottled freeze-dried powder finished product of intestines active peptide of the present invention；Extra large intestines active peptide liquid prepared by step 5 adds pure water by oral
Liquid production technology specification is filling to oral liquid bottle, produces the extra large intestines oral liquid of active peptide finished product of the present invention；Extra large intestines prepared by step 5
Active peptide liquid is freeze-dried or spray drying is prepared into powdery granule, canned into capsule by capsule manufacturing techniques specification, i.e.,
Obtain extra large intestines active peptide capsule finished product of the invention.
Example IV, 1, the preparation of extra large intestines active peptide liquid：Choose fresh and alive extra large intestines 120kg and go internal organ to clean up and broken with tissue
The homogenate of live body sea intestines is made in broken crusher machine；The dirty removal silt of extra large enteral of taking-up is cleaned up and is made with historrhexis's crusher machine
The intestines homogenate of live body sea,
2nd, PH7 is adjusted in extra large intestines homogenate to the live body for preparing step 1 entirely, is added neutral proteinase, papain, trypsase and is carried out
Multienzyme digests, and enzyme ratio of components is 1：1：1, enzyme dosage is that every gram of live body sea intestines add 8000 enzyme-activity units (u), and temperature control exists
56 degree of enzymolysis Celsius add live body sea intestines weight 1% dusty yeast after 6 hours stirs, and temperature control is in 36 degree Celsius progress
Defishying, after 1 hour boiling water heating bath enzyme deactivation 15 minutes, produce extra large intestines enzymolysis liquid；
3rd, the extra large intestines enzymolysis liquid for handling step 2 well carries out melt cinder separation with supercentrifuge, and centrifugal speed is adjusted to 13000rpm
Left and right, the extra large intestines enzymolysis liquid of centrifugation is made；
4th, the extra large intestines enzymolysis liquid for preparing step 3 puts stainless steel cask and enters steam sterilization cabinet in 115 degree of steam high-voltage sterilizings 30 Celsius
Minute；
5th, the extra large intestines enzymolysis liquid for preparing step 4 filters to obtain extra large intestines below the dalton of molecular weight 10000 by ultrafiltration membrane filter
Active peptide liquid；
6th, extra large intestines active peptide liquid prepared by step 5 is done to freeze dryer vacuum refrigeration on cillin bottle by lyophilized technique specification is filling
It is dry, produce the extra large bottled freeze-dried powder finished product of intestines active peptide of the present invention；Extra large intestines active peptide liquid prepared by step 5 adds pure water by oral
Liquid production technology specification is filling to oral liquid bottle, produces the extra large intestines oral liquid of active peptide finished product of the present invention；Extra large intestines prepared by step 5
Active peptide liquid is freeze-dried or spray drying is prepared into powdery granule, canned into capsule by capsule manufacturing techniques specification, i.e.,
Obtain extra large intestines active peptide capsule finished product of the invention.
Claims (3)
1. a kind of preparation method of extra large intestines active peptide, it is characterized in that (1), the fresh and alive extra large intestines of selection go internal organ to clean up use
The homogenate of live body sea intestines is made in historrhexis's crusher machine；The dirty removal silt of extra large enteral of taking-up is cleaned up and broken with historrhexis's machine
The dirty homogenate of broken obtained live body sea enteral；
(2) pH value 6-8, is adjusted into the live body sea intestines homogenate of above-mentioned preparation, adds the 0.1-1% of live body sea intestines weight proteolysis
Enzyme, after digesting 4-8 hours under temperature conditionss between 45-60 degrees Celsius of temperature, add live body sea intestines weight 0.5%-1% yeast
Powder stirs, and temperature control carries out defishying between 30-50 Celsius, after 1-2 hours by temperature be adjusted to 85 degree Celsius with
Upper water-bath 5-15 minutes carry out enzyme deactivation, and extra large intestines enzymolysis liquid is made；
(3) the extra large intestines enzymolysis liquid of above-mentioned preparation, is subjected to melt cinder separation with supercentrifuge, centrifugal speed is adjusted to 8000-14000
Between turning, extra large intestines centrifugation enzymolysis liquid is made；
(4), the extra large intestines of above-mentioned preparation centrifugation enzymolysis liquid is put into stainless steel cask enter steam sterilization cabinet between 100-120 degree Celsius and steam
Vapour autoclaving 15-40 minutes；
(5) the extra large intestines enzymolysis liquid of above-mentioned preparation, is filtered into obtain extra large intestines below the dalton of molecular weight 10000 by ultrafiltration membrane filter
Active peptide liquid；
(6), the extra large intestines active peptide liquid of above-mentioned preparation is done to freeze dryer vacuum refrigeration on cillin bottle by lyophilized technique specification is filling
It is dry, produce the extra large bottled freeze-dried powder finished product of intestines active peptide of the present invention；The extra large intestines active peptide liquid of above-mentioned preparation is added into pure water by oral
Liquid production technology specification is filling to oral liquid bottle, produces the extra large intestines oral liquid of active peptide finished product of the present invention；By the extra large intestines of above-mentioned preparation
Active peptide liquid is freeze-dried or spray drying is prepared into powdery granule, canned into capsule by capsule manufacturing techniques specification, i.e.,
Obtain extra large intestines active peptide capsule finished product of the invention.
2. a kind of preparation method of extra large intestines active peptide according to claim 1., it is characterized in that：Described albumen
Hydrolase be neutral proteinase, trypsase, compound protease, flavor protease, one or both of papain with
On combination.
3. a kind of preparation method of extra large intestines active peptide according to claim 1 and 2, it is characterized in that：Described sea
Intestines active peptide liquid is filling to freeze dryer vacuum freeze drying on cillin bottle by lyophilized technique specification, produces the extra large intestines active peptide of the present invention
Bottled freeze-dried powder product；Described extra large intestines active peptide liquid adds water to be produced into extra large intestines active peptide mouth by oral liquid process specification
Take liquid product；Described extra large intestines active peptide liquid is freeze-dried or spray drying is prepared into powdery granule, by capsule manufacture work
Skill specification is canned into capsule, produces the extra large intestines active peptide capsule product of the present invention.
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| CN109567122A (en) * | 2018-11-28 | 2019-04-05 | 金葵食品科技（大连）股份有限公司 | Natural fresh adding compound seasoner of a kind of sea intestines and preparation method thereof |
| CN113122604A (en) * | 2021-05-25 | 2021-07-16 | 山东大学 | Sea intestine glue and preparation method and application thereof |
| CN113583084A (en) * | 2021-07-28 | 2021-11-02 | 中日友好医院(中日友好临床医学研究所) | Urechis unicinctus leach liquor protein extract and extraction method and application thereof |
| CN113826907A (en) * | 2020-06-24 | 2021-12-24 | 青岛大螠海创生物科技有限公司 | A method for preparing solid beverage containing Stichopus japonicus peptide, intestinum Stichopi Japonici peptide and Concha Ostreae peptide |
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| CN108477442A (en) * | 2018-02-11 | 2018-09-04 | 浙江省海洋开发研究院 | A kind of Urechis uniconctus thrombus dissolving functional health beverage and preparation method thereof |
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| CN113826907A (en) * | 2020-06-24 | 2021-12-24 | 青岛大螠海创生物科技有限公司 | A method for preparing solid beverage containing Stichopus japonicus peptide, intestinum Stichopi Japonici peptide and Concha Ostreae peptide |
| CN113122604A (en) * | 2021-05-25 | 2021-07-16 | 山东大学 | Sea intestine glue and preparation method and application thereof |
| CN113583084A (en) * | 2021-07-28 | 2021-11-02 | 中日友好医院(中日友好临床医学研究所) | Urechis unicinctus leach liquor protein extract and extraction method and application thereof |
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Application publication date: 20171215 |