CN107422091A - A kind of direct drinking fountain of novel intelligent monitoring water quality - Google Patents
A kind of direct drinking fountain of novel intelligent monitoring water quality Download PDFInfo
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- CN107422091A CN107422091A CN201710664041.4A CN201710664041A CN107422091A CN 107422091 A CN107422091 A CN 107422091A CN 201710664041 A CN201710664041 A CN 201710664041A CN 107422091 A CN107422091 A CN 107422091A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/18—Water
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- A—HUMAN NECESSITIES
- A47—FURNITURE; DOMESTIC ARTICLES OR APPLIANCES; COFFEE MILLS; SPICE MILLS; SUCTION CLEANERS IN GENERAL
- A47J—KITCHEN EQUIPMENT; COFFEE MILLS; SPICE MILLS; APPARATUS FOR MAKING BEVERAGES
- A47J31/00—Apparatus for making beverages
- A47J31/44—Parts or details or accessories of beverage-making apparatus
- A47J31/4403—Constructional details
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56911—Bacteria
- G01N33/56916—Enterobacteria, e.g. shigella, salmonella, klebsiella, serratia
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- A—HUMAN NECESSITIES
- A47—FURNITURE; DOMESTIC ARTICLES OR APPLIANCES; COFFEE MILLS; SPICE MILLS; SUCTION CLEANERS IN GENERAL
- A47J—KITCHEN EQUIPMENT; COFFEE MILLS; SPICE MILLS; APPARATUS FOR MAKING BEVERAGES
- A47J2201/00—Devices having a modular construction
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
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- General Physics & Mathematics (AREA)
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- Urology & Nephrology (AREA)
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- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
Abstract
The invention particularly discloses a kind of direct drinking fountain of novel intelligent monitoring water quality, including monitoring water quality module, the monitoring water quality module includes central processing unit, dissolved oxygen detection module, physical and chemical parameter detection module, microorganism detection module, warning system and control panel；The dissolved oxygen detection module, physical and chemical parameter detection module, microorganism detection module, warning system and control panel electrically connect with central processing unit, and the central processing unit also includes memory module and communication module；The monitoring water quality module electrically connects with direct drinking fountain main frame.
Description
Technical field
The present invention relates to direct drinking fountain technical field, in particular it relates to a kind of direct drinking fountain of novel intelligent monitoring water quality.
Background technology
Direct drinking is by the water in tap water pipe line, directly being drunk for people again after carrying out advanced treating.Because of its warp
Ji, conveniently, Huimin the features such as, quickly grown in recent years in China.Direct drinking fountain is typically mounted at public place, such as school, doctor
Institute, enterprises and institutions and sight spot park etc., direct drinking fountain have with water it is more convenient the characteristics of.It sends out from initial vertical water dispenser
The more convenient direct drinking fountain of use with multi-filtering device and sterilizing unit of exhibition till now.Due to direct drinking fountain power supply
Be in opening throughout the year, i.e., it is throughout the year in running order；The equipment of direct drinking fountain and caused water are easily received micro-
The pollution of biology, whether the effluent quality of direct drinking fountain up to standard, if safety, is directly connected to and vast is good for using the body of crowd
Health.Therefore, provided that the problem of direct drinking of safety approval is one extremely important.
The content of the invention
For the above-mentioned deficiency of prior art, the present invention provides a kind of direct drinking fountain of novel intelligent monitoring water quality, with solution
Certainly above-mentioned technical problem.
The present invention is achieved by the following technical solutions：
A kind of direct drinking fountain of novel intelligent monitoring water quality, including monitoring water quality module, the monitoring water quality module include
Central processing unit, dissolved oxygen detection module, physical and chemical parameter detection module, microorganism detection module, warning system and control panel；
The dissolved oxygen detection module, physical and chemical parameter detection module, microorganism detection module, warning system and control panel with center
Processor electrically connects, and the central processing unit also includes memory module and communication module；The monitoring water quality module and direct drinking
Owner's mechatronics.
Brief description of the drawings
Fig. 1 is the structural representation of the present invention.
Fig. 2 is the Escherichia coli colloid gold test paper structural representation in the present invention.
Wherein：Sample layer A, colloid gold label layer B, detection conversion zone C, water accepting layer D, detection line X, nature controlling line Y.
Embodiment
The invention will be further described with the following Examples.
A kind of direct drinking fountain of novel intelligent monitoring water quality, as shown in figure 1, including monitoring water quality module, the water quality prison
Controlling module includes central processing unit, dissolved oxygen detection module, physical and chemical parameter detection module, microorganism detection module, warning system
And control panel；The dissolved oxygen detection module, physical and chemical parameter detection module, microorganism detection module, warning system and control
Panel electrically connects with central processing unit, and the central processing unit also includes memory module and communication module；The monitoring water quality
Module electrically connects with direct drinking fountain main frame.
The direct drinking fountain of the novel intelligent monitoring water quality of the present invention incorporates a variety of parameters to be detected and entered in a system
Row detection, integrate, finally make a report.And detection process is according to default scope before each parameter, if detection
Some parameter of process will start work beyond preset range, warning system, send corresponding alarm, remind user to make phase
The adjustment or measure answered, to ensure that the water quality of water outlet meets production requirement, avoid endangering consumer because of the deterioration of water quality
The health of group.
Preferably, the communication module is wireless communication module；The wireless communication module include WIFI communication module or
Person's bluetooth communication module；The warning system includes voice broadcast unit and LED flashing lamps.
Preferably, the system is connected by communication module with client wireless, the client include smart mobile phone or
Person's tablet personal computer.
Preferably, the dissolved oxygen detection module, physical and chemical parameter detection module, microorganism detection module are equipped with accordingly
Information acquisition module, the testing result for gathering the module upload to central processing unit and carry out processing integration；The central processing unit
For single-chip microcomputer.
Preferably, the physical and chemical parameter detection module includes pH detection sensors and temperature sensor；The microorganism inspection
It is bacterial content detection sensor or biochip to survey module.
It is highly preferred that the microorganism detection module is made up of a kind of Escherichia coli colloid gold test paper.
As preferred embodiment, structure and the work of the Escherichia coli colloid gold test paper of the present invention are will be described in detail below
Principle.
A kind of Escherichia coli colloid gold test paper, as shown in Fig. 2 the test paper includes sample layer A, colloid gold label layer B, inspection
Conversion zone C, water accepting layer D are surveyed, anticolibacillary antibody is coated with the colloid gold label layer, is set in the detection conversion zone
Detection line X and nature controlling line Y are equipped with, bacillus coli antigen is coated with the detection line, the nature controlling line is coated with goat-anti rabbit
IgG, the detection gold labeling antibody can be with detection envelope antigen association reaction and developing the color, and in detection gold labeling antibody only
There is a site combined with antigen-specific；The sample layer, colloid gold label layer and detection conversion zone according to from the inside to the outside, from
Order under up to is incorporated on backer board the same face successively, and colloid gold label layer is overlapping with the end of detection conversion zone；It is described
Water accepting layer is incorporated in the other end of detection conversion zone.
The present invention is applied in the detection technique of biotechnology using air bladder as biofilm structure first, and air bladder possesses nitric acid
The basic structure of cellulose membrane and some standard biologic films, meets the basic demand of biomembrane, and the source of air bladder is easy, cost
It is cheap, apply it in biological products and a kind of high-quality biomembrane, greatly reduce film part in biological products into
This, increases economic efficiency.
Preferably, it is positive mixed to include Gram-negative bacteria, gram-positive bacteria or Gram-negative for the Escherichia coli
Close bacterium.
The strict ratio for controlling colloid gold labeling antibody, bacillus coli antigen and goat anti-rabbit igg of the invention.During concrete application,
Can according to different detection line or parameter line whether develop the color and the depth of color, judge sample liquid in whether contain large intestine bar
Bacterium, and content are more with less, realizing half-quantitative detection.
Preferably, the detection line is 1~4, and nature controlling line is 1~3.
It is highly preferred that the detection line is 1~3, nature controlling line is 1~2.
Preferably, anti-Escherichia coli antibody is coated with the colloid gold label layer, is made with escherichia coli outer membrane protein
For what is prepared after antigen-immunized animal.
Preferably, the sample layer is made up of the good material of water imbibition.
Preferably, the backer board is made up of plastics.
Preferably, the colloid gold label layer and the material of detection conversion zone are biomembrane prepared by air bladder.
It is highly preferred that the birds egg shell membrane is biomembrane prepared by black carp, grass carp, silver carp and bighead.
It is highly preferred that the colloid gold label layer is biomembrane prepared by black carp, grass carp or silver carp, the detection conversion zone
The biomembrane prepared for grass carp, silver carp or bighead.
What colloid gold label layer and detection conversion zone in the colloid gold test paper of the present invention were prepared using air bladder for raw material
Biomembrane, air bladder film have good gas permeability and water penetration, cheap and easy to get, high mechanical strength and good toughness, can be enzyme
Immobilization provides excellent bio-matrix.
When two detection lines X1 and X2 show red, then sample is negative (i.e. without Escherichia coli)；As detection line X1
Colour developing, when detection line X2 does not develop the color, then contains Escherichia coli, but its concentration is not high in sample；When two detection lines do not develop the color
When, then in sample Escherichia coli it is dense, half-quantitative detection is carried out with this.
When detection line X colors are deeper than reference line Y3, then sample is negative (without Escherichia coli or denier)；Work as detection
When line X colors are deeper than Y3 and are shallower than reference line Y2, then sample contains Escherichia coli, and concentration ratio is relatively low；When detection line X colors are deeper than Y2
When being shallower than Y1, then contain Escherichia coli in sample, concentration is higher；When detection line X does not develop the color, then contain large intestine bar in sample
Bacterium, concentration is very high, and half-quantitative detection is carried out with this.
As a result criterion：
The effect of nature controlling line is：Whether no matter Escherichia coli, colloid gold label layer coated gold mark is contained in sample solution
Antibody moves up can be combined when reaching nature controlling line with the IgG on nature controlling line, and aggregation forms macroscopic red granules, Quality Control
Line develops the color all the time, if nature controlling line does not develop the color, represents this test invalidation.
Preferably embodiment, the method that air bladder is applied to biomembrane is will be described in detail below, is comprised the following steps：
S1. the air bladder moisturizing that will be collected into, it is specially that periodically 37 DEG C of deionized waters of spray, subsequent operation process keep air bladder
Keep the state of moistening；
S2. lightly hemispherical air bladder is cut along warp, shakeout, i.e., planarized air bladder；
S3. the air bladder of above-mentioned steps is placed in beaker, adds appropriate amount of deionized water, beaker is placed on oscillator, 46
DEG C, 100~120rpm, 1~2h is vibrated, after vibration terminates, egg shell membrane is placed in the slow water of flowing and rinsed, removes film surface
Impurity；
S4. the air bladder of above-mentioned steps is placed in plane, air bladder is layered outer membrane and inner membrance using tweezers；
S5. outer membrane is placed in 50mMTris-HCl (pH7.5), 10mM CaCl2In solution, it is placed on magnetic stirring apparatus, incubates
40~55 DEG C, 30min are educated, above-mentioned solution adds 20mg/ml Proteinase K 100ul per 100ml, continues 10~20min of incubation；
S6. inner membrance is placed in 50mMTris-HCl (pH7.0), 10mM CaCl2In solution, it is placed on magnetic stirring apparatus, incubates
40~55 DEG C, 10min are educated, above-mentioned solution adds 20mg/ml Proteinase K 10ul per 100ml, continues 5~10min of incubation；
S7. after step S5 and S6 reactions terminate, quickly remove, be placed in 3~5min of flushing in the running water of flowing, make afterwards
With 75% alcohol washes 3~5 times, each 5min；
S8. the film of above-mentioned steps is cleaned 3~5 times, each 3min using deionized water, and 4 DEG C after terminating, moisturizing preserves standby
With.
The present invention using colloid gold test paper of the air bladder as membrane structure, only one and antigen-specific knot in gold labeling antibody
The site of conjunction, there is high specificity；High sensitivity, positive rate basically reach 100%, and detection speed is fast, flux is big, simple to operate
Convenience, colour stable, the advantages that detection temperature optimum range is wide, test paper long shelf-life and cost is cheap, and semidefinite can be realized
Amount detection.The Rapid detection test strip all can be used at 4~50 DEG C, and 5~8min can observe result later, be suitable for food medicine
The departments such as product, environmental protection, inspection and quarantine are used for quickly detecting to the gram- bacteria in sample.
Finally it should be noted that the above embodiments are merely illustrative of the technical solutions of the present invention, rather than the present invention is protected
The limitation of scope is protected, although being explained with reference to preferred embodiment to the present invention, one of ordinary skill in the art should
Work as understanding, technical scheme can be modified or equivalent substitution, without departing from the reality of technical solution of the present invention
Matter and scope.
Claims (6)
- A kind of 1. direct drinking fountain of novel intelligent monitoring water quality, it is characterised in that including monitoring water quality module, the monitoring water quality Module include central processing unit, dissolved oxygen detection module, physical and chemical parameter detection module, microorganism detection module, warning system and Control panel；The dissolved oxygen detection module, physical and chemical parameter detection module, microorganism detection module, warning system and chain of command Plate electrically connects with central processing unit, and the central processing unit also includes memory module and communication module；The monitoring water quality mould Block electrically connects with direct drinking fountain main frame.
- 2. the direct drinking fountain of novel intelligent according to claim 1 monitoring water quality, it is characterised in that the communication module is Wireless communication module；The wireless communication module includes WIFI communication module or bluetooth communication module；The warning system bag Include voice broadcast unit and LED flashing lamps.
- 3. the direct drinking fountain of novel intelligent monitoring water quality according to claim 2, it is characterised in that the system passes through logical Letter module is connected with client wireless, and the client includes smart mobile phone or tablet personal computer.
- 4. the direct drinking fountain of novel intelligent monitoring water quality according to claim 3, it is characterised in that the dissolved oxygen detection Module, physical and chemical parameter detection module, microorganism detection module are equipped with corresponding information acquisition module, gather the detection of the module As a result upload to central processing unit and carry out processing integration；The central processing unit is single-chip microcomputer.
- 5. the direct drinking fountain of novel intelligent monitoring water quality according to claim 4, it is characterised in that the physical and chemical parameter inspection Surveying module includes pH detection sensors and temperature sensor；The microorganism detection module be bacterial content detection sensor or Biochip.
- 6. the direct drinking fountain of novel intelligent monitoring water quality according to claim 5, it is characterised in that the microorganism detection Module is made up of a kind of Escherichia coli colloid gold test paper；The test paper includes sample layer A, colloid gold label layer B, detection conversion zone C, water accepting layer D, anticolibacillary antibody is coated with the colloid gold label layer, detection is provided with the detection conversion zone Line X and nature controlling line Y, bacillus coli antigen is coated with the detection line, the nature controlling line is coated with goat anti-rabbit igg, the inspection Survey gold labeling antibody can only have with detection envelope antigen association reaction and developing the color, and in detection gold labeling antibody one with it is anti- The site of former specific bond；The sample layer, colloid gold label layer and detection conversion zone are suitable according to from the inside to the outside, from top to bottom Sequence is incorporated on backer board the same face successively, and colloid gold label layer is overlapping with the end of detection conversion zone；The water accepting layer combines In the other end of detection conversion zone.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710664041.4A CN107422091A (en) | 2017-08-06 | 2017-08-06 | A kind of direct drinking fountain of novel intelligent monitoring water quality |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710664041.4A CN107422091A (en) | 2017-08-06 | 2017-08-06 | A kind of direct drinking fountain of novel intelligent monitoring water quality |
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| Publication Number | Publication Date |
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| CN107422091A true CN107422091A (en) | 2017-12-01 |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104391098A (en) * | 2014-12-01 | 2015-03-04 | 成都蓝宇科维科技有限公司 | DCS-based water quality monitoring equipment |
| CN105640347A (en) * | 2016-03-25 | 2016-06-08 | 李�杰 | Water dispenser control system |
| CN205898782U (en) * | 2016-07-29 | 2017-01-18 | 周明哲 | Water quality detection device |
| CN106841185A (en) * | 2017-01-21 | 2017-06-13 | 上海巽晔计算机科技有限公司 | A kind of monitoring water quality on line system of breeding water body |
| CN106841602A (en) * | 2017-01-21 | 2017-06-13 | 上海源紊新能源科技有限公司 | A kind of diallyl phthalate colloidal gold immunochromatographydetection detection test paper |
-
2017
- 2017-08-06 CN CN201710664041.4A patent/CN107422091A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104391098A (en) * | 2014-12-01 | 2015-03-04 | 成都蓝宇科维科技有限公司 | DCS-based water quality monitoring equipment |
| CN105640347A (en) * | 2016-03-25 | 2016-06-08 | 李�杰 | Water dispenser control system |
| CN205898782U (en) * | 2016-07-29 | 2017-01-18 | 周明哲 | Water quality detection device |
| CN106841185A (en) * | 2017-01-21 | 2017-06-13 | 上海巽晔计算机科技有限公司 | A kind of monitoring water quality on line system of breeding water body |
| CN106841602A (en) * | 2017-01-21 | 2017-06-13 | 上海源紊新能源科技有限公司 | A kind of diallyl phthalate colloidal gold immunochromatographydetection detection test paper |
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Application publication date: 20171201 |