CN107372480A - A kind of polymyxins biological pesticide solution for preventing and treating plant soil-borne diseases - Google Patents
A kind of polymyxins biological pesticide solution for preventing and treating plant soil-borne diseases Download PDFInfo
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- CN107372480A CN107372480A CN201710590984.7A CN201710590984A CN107372480A CN 107372480 A CN107372480 A CN 107372480A CN 201710590984 A CN201710590984 A CN 201710590984A CN 107372480 A CN107372480 A CN 107372480A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/02—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents
- A01N25/04—Dispersions, emulsions, suspoemulsions, suspension concentrates or gels
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/22—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing ingredients stabilising the active ingredients
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/36—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/713—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with four or more nitrogen atoms as the only ring hetero atoms
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/10—Apiaceae or Umbelliferae [Carrot family], e.g. parsley, caraway, dill, lovage, fennel or snakebed
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/24—Lauraceae [Laurel family], e.g. laurel, avocado, sassafras, cinnamon or camphor
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/28—Myrtaceae [Myrtle family], e.g. teatree or clove
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/40—Liliopsida [monocotyledons]
- A01N65/42—Aloeaceae [Aloe family] or Liliaceae [Lily family], e.g. aloe, veratrum, onion, garlic or chives
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- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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Abstract
The invention provides a kind of polymyxins biological pesticide solution for preventing and treating plant soil-borne diseases, the biological pesticide solution is made up of the raw material of following percentage by weight：Polymyxins 5 30%, plants essential oil 5 12%, surfactant 8 15%, citric acid 0.005 0.02%, water surplus；The polymyxins is one kind in polymyxin B, aerosporin, polymyxin e, polymyxin E sulfate；The plants essential oil is one or more kinds of mixing in galic essential oil, eucalyptus leaf essential oil, Peppermint essential oil, litsea cubeba oil, cumin essential oil, lemon；The present invention is compounded polymyxins and plants essential oil, it is used to prepare biological pesticide solution together, both synergies, there can be good preventive and therapeutic effect to a variety of soil infection diseases, it is particularly notable to the prevention effect of tobacco black shank, cabbage clubroot, notoginseng root rot, ginseng root rot, has a broad antifungal spectrum, antimicrobial efficiency are high.
Description
Technical field
The present invention relates to technical field of pesticide, and in particular to a kind of polymyxins biological pesticide for preventing and treating plant soil-borne diseases
Aqua.
Background technology
Plant soil-borne diseases, refer to that pathogen such as fungi, bacterium, nematode and virus are lived in soil with invalid body, bar
From disease caused by crop root or stem infringement crop when part is suitable.Infecting cause of disease includes fungi, bacterium, actinomyces, line
Worm etc..Wherein based on fungi, it is divided into non-obligatory parasitism and the class of obligatory parasitism two.Non- obligatory parasitism is that external root infects fungi,
As pythium spp causes, seedling is rotten and samping off, rhizoctonia cause seedling blight.Obligatory parasitism is Microtubules in plants beam disease fungus, typical case
Wilted as sharp spore sickle, Huang wither caused by wheel branch spore etc., be withered.The order of severity of root disease is by butt secretion composition and concentration
Left and right.Therefore, the activity of rhizosphere system pathogen is suppressed with regard to turning into protection root system and carrying out the basis of soil-borne disease preventing and treating.But must
It must pay attention to and consider that restriction of the soil physical chemistry factor to correlation between plant, edaphon and root pathogen three is made
With.
Wherein tobacco black shank is one of most destructive disease on tobacco leaf production, and also known as tobacco epidemic disease, tobacco grower are referred to as
" black bar is crazy ", " black root ", " rhizome of Chinese monkshood disease ".Each main production cigarette district of China has different degrees of generation, wherein Anhui, Shandong, Henan
Province is historical grave illness area；The Main Tobacco-growing Regions In Souths such as Yunnan, Guizhou, Sichuan, Hunan, Guangdong, Guangxi, Fujian occur also quite general
Time, and generation is mixed with tobacco bacterial wilt more, therefore endanger even more serious.The highly effective pesticide one that preventing and treating tobacco black shank uses at present
As for metalaxyl-mn-zn and it is general make every effort to overcome, but the use of chemical synthetic pesticide easily produces residues of pesticides in soil, and environment is made
Into adverse effect, and its preventive effect stills need further to improve.
Polymyxins is to be found in more glutinous series bacillus, the antibiotic property polypeptide in nutrient solution, there is A, B, C, D, E etc. five
Kind.Antimicrobial spectrum is mutually similar and haves a wide reach, and especially quite strong to gramnegative bacterium effect, toxicity is weaker.Present is more viscous
Rhzomorph is generally used in the treatment of human body diseases, is applied to preventing and treating plant soil-borne diseases for polymyxins directly is made into aqua
In yet there are no relevant report.
The content of the invention
It is an object of the invention to provide a kind of polymyxins biological pesticide solution for preventing and treating plant soil-borne diseases, can be used for
A variety of soil infection diseases are prevented and treated, particularly tobacco black shank, cabbage clubroot, notoginseng root rot, ginseng root rot are prevented
Control significant effect, has a broad antifungal spectrum, antimicrobial efficiency height.
To realize object above, the present invention is achieved by the following technical programs：
A kind of polymyxins biological pesticide solution for preventing and treating plant soil-borne diseases, by the raw material system of following percentage by weight
Into：Polymyxins 5-30%, plants essential oil 5-12%, surfactant 8-15%, citric acid 0.005-0.02%, water surplus.
Preferably, the polymyxins is polymyxin B, aerosporin, polymyxin e, polymyxin E sulfate
In one kind.It is furthermore preferred that the polymyxins is polymyxin B or aerosporin.
Preferably, the plants essential oil be galic essential oil, eucalyptus leaf essential oil, Peppermint essential oil, litsea cubeba oil, cumin essential oil,
One or more kinds of mixing in lemon.
Preferably, the surfactant be DBSA, dodecyl hydroxypropyl sulfobetaines, tween-
40th, one or more kinds of mixtures in Tween-80, NP-10, OP-10.It is highly preferred that the surfactant is 12
Alkyl hydroxypropyl base sulfobetaines, Tween-80, NP-10 in mass ratio 10：2-8：3-6 is mixed.
Preferably, it is made up of the raw material of following percentage by weight：Polymyxins 18%, plants essential oil 10%, surface-active
Agent 12%, citric acid 0.01%, water surplus.
The preparation method of the polymyxins biological pesticide solution of the above-mentioned preventing and treating plant soil-borne diseases of the present invention includes following step
Suddenly：Water is added into surfactant under agitation, adds polymyxins and lemon after being completely dissolved in the case where continuously stirring again
Lemon acid, is stirring evenly and then adding into plants essential oil, continues stirring untill liquid-transparent, produces.
The beneficial effects of the invention are as follows：
Polymyxins is applied to preventing and treating plant soil-borne diseases by the present invention, and its has a broad antifungal spectrum, antimicrobial efficiency is high, especially to leather
Gram-negative bacteria effect is quite strong, and low toxic and environment-friendly.It is thin that cyclic structure wherein in polymyxins molecule is inserted into bacterium
In after birth, so as to change cell permeability of the membrane, causing the loss of cell inclusion causes death.Add in biological pesticide solution
The plants essential oil entered, it can be by destroying the integrality of cell membrane and cell membrane, the synthesis of suppression albumen and nucleic acid, influence energy
Metabolism and reduction enzyme system, the approach such as lipid layer is stable, fat-body in dissolving cell are influenceed to realize the suppression to bacterium.
On this basis, the present invention is compounded polymyxins and plants essential oil, is used to prepare biological pesticide water together
Agent, both act synergistically, and can have good preventive and therapeutic effect to a variety of soil infection diseases, particularly to tobacco black shank, in vain
Dish clubroot, notoginseng root rot, the prevention effect of ginseng root rot are notable.The present invention adds suitable in biological pesticide solution simultaneously
The citric acid of amount, the stability of aqua can be not only effectively improved, while the addition of citric acid can also further strengthen aqua
The effect of preventing and treating soil infection disease.
Embodiment
To make the purpose, technical scheme and advantage of the embodiment of the present invention clearer, below in conjunction with the embodiment of the present invention,
Technical scheme in the embodiment of the present invention is clearly and completely described, it is clear that described embodiment is the present invention one
Divide embodiment, rather than whole embodiments.Based on the embodiment in the present invention, those of ordinary skill in the art are not making
The every other embodiment obtained under the premise of creative work, belongs to the scope of protection of the invention.
In the embodiment of the present invention, a kind of polymyxins biological pesticide solution for preventing and treating plant soil-borne diseases, by following weight
The raw material of percentage is made：Polymyxins 5-30%, plants essential oil 5-12%, surfactant 8-15%, citric acid 0.005-
0.02%th, water surplus.It is further preferred that the biological pesticide solution is made up of the raw material of following percentage by weight：Polymyxins
18%th, plants essential oil 10%, surfactant 12%, citric acid 0.01%, water surplus.
Polymyxins is in polymyxin B, aerosporin, polymyxin e, polymyxin E sulfate in the present invention
It is a kind of.It is furthermore preferred that the polymyxins is polymyxin B or aerosporin.
Plants essential oil is galic essential oil, eucalyptus leaf essential oil, Peppermint essential oil, litsea cubeba oil, cumin essential oil, lemon in the present invention
One or more kinds of mixing in essential oil.Surfactant is DBSA, dodecyl hydroxypropyl sulfo group sweet tea
One or more kinds of mixtures in dish alkali, Tween-40, Tween-80, NP-10, OP-10；It is highly preferred that the surface-active
Agent is dodecyl hydroxypropyl sulfobetaines, Tween-80, NP-10 in mass ratio 10：2-8：3-6 is mixed.
The preparation method of the polymyxins biological pesticide solution of present invention preventing and treating plant soil-borne diseases comprises the following steps：Will
Water adds surfactant under agitation, adds polymyxins and citric acid after being completely dissolved in the case where continuously stirring again,
Plants essential oil is stirring evenly and then adding into, continues stirring untill liquid-transparent, produces.
Embodiment 1：
A kind of polymyxins biological pesticide solution for preventing and treating plant soil-borne diseases, by the raw material system of following percentage by weight
Into：Aerosporin 18%, litsea cubeba oil 10%, surfactant 12%, citric acid 0.01%, water surplus.
Above-mentioned surfactant is dodecyl hydroxypropyl sulfobetaines, Tween-80, NP-10 in mass ratio 10：5：4
Mix.
Contrast control test：
Tobacco black shank is prevented and treated using the biological pesticide solution in embodiment 1 and conventional method：
Using the prevention and controls of the biological pesticide solution of embodiment 1：Biological pesticide solution 500 in embodiment 1 is used during transplanting
Times liquid carries out root dipping processing, and after transplanting 15 days, tank root processing are carried out using 1500 times of liquid of biological pesticide solution in embodiment 1.
Conventional control method：Sprinkling 72% is general after transplanting makes every effort to overcome 500 times of wettable powder liquid 1 time；After cigarette strain group, often
Mu is watered 55kg sprays with 58% metalaxyl-mn-zn 100g, decoction is seeped into along cauline leaf stream in the topsoil around foundation.
5 groups of model fields and 1 group of control field are set, and model field 1, model field 2, model field 3 are according to the biological pesticide water of embodiment 1
Agent prevention and controls are prevented and treated tobacco black shank, and model field 4, model field 5, model field 6 are black to tobacco by above-mentioned conventional method
Shin disease is prevented and treated, and control field is without any prophylactico-therapeutic measures.
Investigation method：100 plants of tobaccos of model field and control Tanaka are adjusted comprehensively simultaneously after the stable disease of field
Look into, obtain the incidence of disease, calculate relative control effect (%)：
The incidence of disease (%)=diseased plant number/investigation strain number × 100
Relative control effect (%)=(the control field incidence of disease-model field incidence of disease)/control field incidence of disease × 100
As a result it is as shown in table 1：
Relative control effect of the different disposal method of table 1 to tobacco black shank：
From table 1 can it was found from, in embodiment 1 polymyxins biological pesticide solution preventing and treating tobacco black shank positive effect compared with
Conventional method is good, and its relative control effect can reach 86.9%.In addition, by the colistin biological pesticide solution usual manner pair
When cabbage clubroot, notoginseng root rot, ginseng root rot are prevented and treated, its relative control effect can reach more than 84%.
Embodiment 2：
A kind of polymyxins biological pesticide solution for preventing and treating plant soil-borne diseases, by the raw material system of following percentage by weight
Into：Coly-Mycin S 30%, galic essential oil 8%, table dodecyl hydroxypropyl sulfobetaines 10%, citric acid 0.02%,
Water surplus.
Embodiment 3：
A kind of polymyxins biological pesticide solution for preventing and treating plant soil-borne diseases, by the raw material system of following percentage by weight
Into：Polymyxin E sulfate 20%, litsea cubeba oil 6%, lemon 6%, DBSA 15%, citric acid
0.005%th, water surplus.
Embodiment 4：
A kind of polymyxins biological pesticide solution for preventing and treating plant soil-borne diseases, by the raw material system of following percentage by weight
Into：Polymyxin B 15%, cumin essential oil 5%, Tween-80 10%, NP-10 3%, citric acid 0.015%, water surplus.
Embodiment 5：
A kind of polymyxins biological pesticide solution for preventing and treating plant soil-borne diseases, by the raw material system of following percentage by weight
Into：Polymyxin e 5%, eucalyptus leaf essential oil 6%, Tween-40 8%, citric acid 0.01%, water surplus.
The above embodiments are merely illustrative of the technical solutions of the present invention, rather than its limitations；Although with reference to the foregoing embodiments
The present invention is described in detail, it will be understood by those within the art that：It still can be to foregoing each implementation
Technical scheme described in example is modified, or carries out equivalent substitution to which part technical characteristic；And these modification or
Replace, the essence of appropriate technical solution is departed from the spirit and scope of various embodiments of the present invention technical scheme.
Claims (8)
1. a kind of polymyxins biological pesticide solution for preventing and treating plant soil-borne diseases, it is characterised in that by following percentage by weight
Raw material be made：Polymyxins 5-30%, plants essential oil 5-12%, surfactant 8-15%, citric acid 0.005-0.02%,
Water surplus.
2. the polymyxins biological pesticide solution of described preventing and treating plant soil-borne diseases according to claim 1, its feature
It is, the polymyxins is one kind in polymyxin B, aerosporin, polymyxin e, polymyxin E sulfate.
3. the polymyxins biological pesticide solution of preventing and treating plant soil-borne diseases according to claim 1, it is characterised in that institute
It is polymyxin B or aerosporin to state polymyxins.
4. the polymyxins biological pesticide solution of preventing and treating plant soil-borne diseases according to claim 1, it is characterised in that institute
State plants essential oil for galic essential oil, eucalyptus leaf essential oil, Peppermint essential oil, litsea cubeba oil, cumin essential oil, one kind in lemon or
More than one mixing.
5. the polymyxins biological pesticide solution of preventing and treating plant soil-borne diseases according to claim 1, it is characterised in that institute
State surfactant for DBSA, dodecyl hydroxypropyl sulfobetaines, Tween-40, Tween-80, NP-10,
One or more kinds of mixtures in OP-10.
6. the polymyxins biological pesticide solution of preventing and treating plant soil-borne diseases according to claim 5, it is characterised in that institute
Surfactant is stated as dodecyl hydroxypropyl sulfobetaines, Tween-80, NP-10 in mass ratio 10：2-8：3-6 mix and
Into.
7. the polymyxins biological pesticide solution of preventing and treating plant soil-borne diseases according to claim 1, it is characterised in that by
The raw material of following percentage by weight is made：Polymyxins 18%, plants essential oil 10%, surfactant 12%, citric acid
0.01%th, water surplus.
8. the polymyxins biological pesticide solution of the preventing and treating plant soil-borne diseases according to claim any one of 1-7, it is special
Sign is that its preparation method comprises the following steps：Water is added into surfactant under agitation, continuously stirred again after being completely dissolved
Polymyxins and citric acid are added in the case of mixing, is stirring evenly and then adding into plants essential oil, continues stirring until liquid-transparent is
Only, produce.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110845269A (en) * | 2019-12-02 | 2020-02-28 | 南宁汉和生物科技股份有限公司 | Preparation method of polymyxin-containing preparation for inhibiting soil-borne diseases |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110845269A (en) * | 2019-12-02 | 2020-02-28 | 南宁汉和生物科技股份有限公司 | Preparation method of polymyxin-containing preparation for inhibiting soil-borne diseases |
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