CN1073562C - Method for extracting material containing raw anthocyanidin from ginkgo leaf by supercritical carbon dioxide - Google Patents
Method for extracting material containing raw anthocyanidin from ginkgo leaf by supercritical carbon dioxide Download PDFInfo
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- CN1073562C CN1073562C CN99100588A CN99100588A CN1073562C CN 1073562 C CN1073562 C CN 1073562C CN 99100588 A CN99100588 A CN 99100588A CN 99100588 A CN99100588 A CN 99100588A CN 1073562 C CN1073562 C CN 1073562C
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- procyanidin
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/54—Improvements relating to the production of bulk chemicals using solvents, e.g. supercritical solvents or ionic liquids
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Abstract
The present invention relates to a method for extracting an extract containing procyanidin from ginkgo leaves by using supercritical carbon dioxide and a polarity modifying agent prepared from acetone and water. In the method, under the conditions of the extraction temperature of 60 to 90 DEG C and the extraction pressure of 20 to 35Mpa, a polarity modifying agent prepared from 50 to 80% of acetone and 20 to 50% of water is added for static extraction and dynamic extraction, wherein the extraction time is from 2 to 4 hours; after extraction liquid is concentrated by using traditional resin and is dried by a spray dryer, a refined gingko leaf extract is obtained. In the product, the content of ginkgetin is more than 35%, the content of terpene lactone is more than 8%, the content of procyanidin is more than 7%, and the content of phenolic acid is less than 5 mg/Kg. The method has the advantage of short flow, and the strongest procyanidin used as a natural antioxidant can be extracted by the method.
Description
The present invention relates to a kind of from Ginkgo Leaf the method for extracting procyanidins, particularly a kind of supercritical CO
2Extraction contains the method for procyanidin extract from Ginkgo Leaf.
Ginkgo flavonoid glycoside (Flavoneglycosides), terpene lactones (Terpenelactones) and pycnogenols (Proanthocyanidines) are called for short Folium Ginkgo extract, it is the choice drug that the treatment cardiovascular and cerebrovascular diseases has special effect, particularly pycnogenols is the strongest natural antioxidants found at present (is vitamin-E 50 times), so it has become one of most popular medicinal plants in the world.
At present, produce the method for Folium Ginkgo extract, Germany, France etc. adopt traditional organic solvent extractionprocess, and China then uses alcoholic extraction, wide aperture resin method of enrichment.Not only technology is numerous and diverse, energy consumption is high, yield is low for these methods, dissolvent residual is arranged, and unstable product quality.
Effective ingredient in the Ginkgo Leaf is mainly flavonoid glycoside, terpene lactones and pycnogenols, and oxious component is a ginkgoic acid.The molecular weight of flavonoid glycoside is 500-1000, and polarity is very strong, single CO with low-pole
2Be difficult to extract, must add moisture polarity modifier just can extract, Chinese patent application numbers 97116799.0, proposition adds the method for properties-correcting agent second alcohol and water with supercritical co, make that to contain flavonoid be 37%, the Folium Ginkgo terpene lactones class is 8.2% good result, but extracting procyanidins is not seen that then report is arranged.
The object of the present invention is to provide a kind of supercritical CO
2Extraction contains the method for procyanidin extract from Ginkgo Leaf.
Embodiment of the present invention are:
1, adopts supercritical CO
2Add acetone: polarity modifier extraction ginkgo flavonoid glycoside, terpene lactone and the pycnogenols of water mixture, the weight ratio of acetone and water is 50-80%: 50-20%;
2, processing condition:
Polarity modifier: acetone: water=50-80%: 50-20%;
Extracting pressure: 20-30MPa,
Extraction temperature: 60-80 ℃,
Extraction time: 2-3 hour (static 1 hour, dynamically 1-2 hour);
3, extraction liquid concentrates and spray-dryer is dried to powder-like product through traditional resin tower.
Following preferred row example in conjunction with the accompanying drawings 1 is done detailed description to the present invention, but does not mean limitation of the scope of the invention.Example 1,
To clean, dry, be crushed to the 20 purpose Ginkgo Leaves multistage barrel 5 of packing into, put it into then in the extraction kettle 4, treat that preheater 3, extraction kettle 4 are warmed up to 70 ℃, refrigerator 10 is 3 ± 2 ℃, start properties-correcting agent pump 2, squeeze into moisture polarity modifier acetone and end, start CO to soaking full charging basket
2Pump 1 boosts to 30 ± 5MPa, and static extracting 1 hour utilizes CO
2High pressure, high seepage force, make the Ginkgo Leaf plant cell wall breaking, the stripping effective ingredient; Then, start CO
2Pump 1 and properties-correcting agent pump 2, velocity ratio 80: 20, dynamic extraction 1-2 hour, emit extraction liquid by separator 6,7 by cyclonic separator 8,9, extraction liquid is made dry powder through traditional spray-dryer 12 again after traditional resin tower 11 concentrates, obtain refined ginkgo biloba extract, utilize high performance liquid chromatograph (HPLC) to analyze that content of total flavonol glycosides is 36.2%, procyanidin content is 7.2%, gas chromatograph (GC) analyze terpene lactones content be 8.2% and phenolic acid be 4.2mg/Kg.Example 2,
Remove the acetone of polarity properties-correcting agent and the ratio of water and change 60% into, water 40% outer all the other conditions are constant, make that to contain flavonoid glycoside in the Semen Ginkgo extrac be 35.8%, and containing pycnogenols is 7.3%, and containing terpene lactones is 8.2%, and containing phenolic acid is 4.3mg/Kg.
Advantage of the present invention:
The energy extracting procyanidins, the purity of glycosides displayed and terpene lactone is also high; Secondly, be that flow process simply can Lean on stable operation.
Fig. 1: 1-CO2High-pressure pump, 2-modifier pump, 3-preheater, 4-extraction kettle, 5-multilayer Charging basket, 6,7-separator, 8,9-cyclone separator, 10-refrigerator, 11-resin tower, 12-drying Device.
Claims (1)
1, a kind of employing supercritical co, extraction contains the method for procyanidin extract from Ginkgo Leaf, it is characterized in that:
(1) supercritical CO
2Add acetone and water and make polarity modifier, wherein the weight percent of acetone and water is 50-80%: 50-20%;
(2) extraction process:
Extracting pressure is 20-30MPa,
Extraction temperature is 60-80 ℃,
The extraction time is static 1 hour, dynamically 1-2 hour,
Extraction liquid is made powder through conventional resins tower and spray-drying process.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN99100588A CN1073562C (en) | 1999-02-05 | 1999-02-05 | Method for extracting material containing raw anthocyanidin from ginkgo leaf by supercritical carbon dioxide |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN99100588A CN1073562C (en) | 1999-02-05 | 1999-02-05 | Method for extracting material containing raw anthocyanidin from ginkgo leaf by supercritical carbon dioxide |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1228432A CN1228432A (en) | 1999-09-15 |
| CN1073562C true CN1073562C (en) | 2001-10-24 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN99100588A Expired - Fee Related CN1073562C (en) | 1999-02-05 | 1999-02-05 | Method for extracting material containing raw anthocyanidin from ginkgo leaf by supercritical carbon dioxide |
Country Status (1)
| Country | Link |
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| CN (1) | CN1073562C (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2007006443A1 (en) * | 2005-07-12 | 2007-01-18 | Cognis Ip Management Gmbh | Extracts of ginkgo biloba |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6524628B1 (en) * | 2000-11-06 | 2003-02-25 | Idaho Research Foundation, Inc. | Pressurized water extraction |
| CN1299703C (en) * | 2001-03-28 | 2007-02-14 | 朱耕新 | Process for preparing broad spectrum anti-virus medicine and its use |
| CN1236836C (en) | 2003-12-08 | 2006-01-18 | 曹培生 | A process for extraction and apparatus thereof |
| MX2012005460A (en) | 2009-11-11 | 2012-07-20 | Dynasep Inc | Energy efficient acetone drying method. |
| CN101905090B (en) * | 2010-05-31 | 2012-08-15 | 中国农业大学 | Method for extracting polar or medium polar plant source natural product |
| CN102558128B (en) * | 2011-11-28 | 2015-02-18 | 浙江康恩贝制药股份有限公司 | Preparation method of extract containing prodelphinidin and procyanidin of ginkgo |
| CN102633765B (en) * | 2012-05-18 | 2014-04-30 | 南京林业大学 | Method for extracting procyanidine from folium ginkgo |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4981668A (en) * | 1986-04-29 | 1991-01-01 | Dow Corning Corporation | Silicon carbide as a raw material for silicon production |
| CN1172670A (en) * | 1997-08-22 | 1998-02-11 | 孙云鹏 | Method for extracting flavone and terlactone from ginkgo leaf by supercritical carbon dioxide |
| CN1172669A (en) * | 1997-08-22 | 1998-02-11 | 孙云鹏 | Method for extracting and separating flavone and terlactone from ginkgo leaf by supercritical carbon dioxide |
-
1999
- 1999-02-05 CN CN99100588A patent/CN1073562C/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4981668A (en) * | 1986-04-29 | 1991-01-01 | Dow Corning Corporation | Silicon carbide as a raw material for silicon production |
| CN1172670A (en) * | 1997-08-22 | 1998-02-11 | 孙云鹏 | Method for extracting flavone and terlactone from ginkgo leaf by supercritical carbon dioxide |
| CN1172669A (en) * | 1997-08-22 | 1998-02-11 | 孙云鹏 | Method for extracting and separating flavone and terlactone from ginkgo leaf by supercritical carbon dioxide |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2007006443A1 (en) * | 2005-07-12 | 2007-01-18 | Cognis Ip Management Gmbh | Extracts of ginkgo biloba |
| FR2888509A1 (en) * | 2005-07-12 | 2007-01-19 | Cognis Ip Man Gmbh | EXTRACTS FROM GINKGO BILOBA |
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| Publication number | Publication date |
|---|---|
| CN1228432A (en) | 1999-09-15 |
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