CN107287140B - Environment-friendly enzyme leavening agent and preparation method thereof - Google Patents
Environment-friendly enzyme leavening agent and preparation method thereof Download PDFInfo
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Abstract
The invention discloses an environment-friendly ferment starter and a preparation method thereof, wherein three microorganism strains of bacteria, fungi and actinomycetes are screened and separated from plant rhizosphere microorganisms, then three solid bacteria powders are obtained through strain propagation, liquid state fermentation, centrifugal concentration and spray drying, the three solid bacteria powders are activated and then inoculated into a mixed culture medium for propagation culture, after the three bacterial colonies are mutually nutritious and stable in state, a protective agent is added after the flora is propagated and cultured, and then the environment-friendly ferment starter is obtained through freeze drying, and the composition of the starter comprises the following components in parts by weight: 50-70 parts of bacterial solid bacteria powder, 10-20 parts of fungus solid bacteria powder, 5-15 parts of actinomycete solid bacteria powder, 3-10 parts of protective agent and 5-10 parts of fermentation aid. The invention breaks through the symbiosis problem of different strains, and the obtained microbial floras are mutually nutritious and stable in state by compounding multiple microbial strains, and different microorganisms can have different effects in the environment-friendly ferment, so that the functions of various microorganisms are exerted to the utmost extent.
Description
Technical Field
The invention belongs to the field of microbial leavening agents, and particularly relates to a leavening agent of an environment-friendly enzyme and a preparation method thereof.
Background
The environment-friendly ferment in the market at present is a popular method for producing brown liquid mixed with sugar, water, fruit peel, kitchen waste and the like through natural fermentation, and is named by enzyme in Englishenzyme. The enzyme is a different name of enzyme in Japan and Taiwan of China, and refers to a high molecular substance with a biological catalysis function. Almost all cellular processes require the involvement of enzymes to increase efficiency. Similar to other non-biological catalysts, enzymes accelerate the reaction rate by reducing the activation energy of the chemical reaction. Most enzymes catalyze itThe reaction rate is increased by millions times; in fact, the enzyme provides another way with lower activation energy requirement, so that more reaction particles can possess kinetic energy not less than the activation energy, thereby accelerating the reaction rate. The ferment is used as a catalyst, and is not consumed in the reaction process, and the chemical balance of the reaction is not influenced.
Green development requires hand grips and technical means, and the application of biological enzymes in the fields of planting, breeding, environmental protection and the like has shown great potential. The existing production mode of agriculture and animal husbandry uses a large amount of chemical fertilizers, pesticides and the like, and has great influence on the environment. For the production of agriculture and animal husbandry, the application of the biological ferment technology not only increases the yield, but also can keep the original taste of the product, the naturally fermented ferment has few viable microorganism states, most of the naturally fermented ferment has no viable bacteria, and individual products have viable bacteria, but the number of the naturally fermented ferment is extremely low, and most of the naturally fermented ferment is in a single strain state. Occasionally, two strains are symbiotic on the market, the number of the strains is below one hundred million, and active substances and microbial secondary metabolites are limited. Meanwhile, researches find that a plurality of floras such as saccharomycetes, lactic acid bacteria, bacillus and the like and a plurality of beneficial bacteria are used as biological enzyme sources, and find that the functions of the composite beneficial bacteria are different. Therefore, only by organically combining a plurality of strains according to a certain proportion, the functions of the strains can be brought into full play, and the symbiosis problem of different strains needs to be broken through.
Bacteria can convert complex organic compounds into inorganic compounds that can be utilized by plants and microorganisms; fungi are a particularly important microorganism, generally growing on dead or rotten plants, which cause the decomposition of cellulose, lignin, pectin, etc., the majority of the carbon and nutrient substances produced by its action being utilized by the plants; actinomycetes are active participants for decomposing organic pollutants, and meanwhile, an antibiotic substance is formed to maintain the dynamic balance of wetland biological communities, so that how to organically combine the strains of the microorganisms according to a certain proportion enables the bacterial colonies to be mutually nutritious and stable in state, the functions of the strains can be fully exerted, and the symbiosis problem of different strains needs to be broken through.
At present, the environment-friendly ferment is prepared by natural fermentation without adding any leavening agent, is prepared by fermenting microorganisms carried by the ferment, and is prepared by adding a single strain without mixing floras, and the invention discloses ferment bath foam and a preparation method thereof. Patent numbers: CN201611087719.9 discloses an enzyme bath foam and a preparation method thereof. The invention selects specific sugaring residues and fruit peels as raw materials, prepares an environment-friendly ferment stock solution with definite efficacy by fermenting with a specific yoghourt leaven, and prepares the environment-friendly ferment bath foam with the functions of cleaning and protecting skin, relieving nerves and eliminating fatigue by compounding. Patent "a method for preparing an environment-friendly ferment using litchi shells as main raw material" patent no: CN201410486163.5 discloses a method for preparing an environment-friendly ferment with litchi shells as a main raw material, which comprises the steps of inoculating a mixed strain of red wine yeast, rhodotorula rubra and chestnut wine yeast into fermentation liquor after beating litchi shells, mangosteen skins, lemon skins, longan skins, durian fiddle, jack fruit fiddle, grape skins, pineapple skins and milk, and fermenting; then inoculating lactobacillus bulgaricus and lactobacillus sake to ferment to obtain the environment-friendly enzyme. The patent mainly utilizes yeast and lactobacillus to ferment and prepare the environment-friendly ferment, and also adds the strains stage by stage, firstly adds the yeast, ferments the lactobacillus which is added after a certain time, and does not obtain the flora which are mutually nutritious and have stable state through mixing to ferment.
In summary, the environmental protection enzymes in the prior art are all naturally fermented, or fermented by a single strain, or fermented by different strains in stages, and there is no environmental protection enzyme prepared by fermenting with a flora starter. Therefore, through screening and compounding, microbial species strains are organically matched according to a certain proportion, so that the bacterial colonies are mutually nutritious and have stable states, the functions of the bacterial colonies can be brought into full play, the symbiosis problem of different strains is broken through, and the technical problem which needs to be overcome by technical personnel in the field is solved.
Disclosure of Invention
The invention aims to provide a leavening agent of an environment-friendly enzyme, which breaks through the symbiotic problem of different strains, and is compounded by multiple microbial strains, so that the obtained microbial floras are mutually nutritious and stable in state, and different microorganisms can have different effects in the environment-friendly enzyme, so that the functions of various microorganisms are exerted to the utmost extent.
In order to achieve the purpose, the invention adopts the following technical scheme:
the utility model provides an environmental protection ferment starter, screens out the bacterium through the culture medium of difference from plant rhizosphere microorganism, fungi, three kinds of microbial strains of actinomycete, then expands through the bacterial and cultivates, liquid state fermentation, centrifugal concentration, spray drying obtains three kinds of solid fungus powder, inoculates the mixed culture medium after three kinds of solid fungus powder activation and enlarges and cultivates, treats each other for nutrition and state stability between three kinds of bacterial colony, to the fungus crowd expand cultivates the back, adds protective agent after freeze drying obtains the environmental protection ferment starter, the composition of environmental protection ferment starter include by weight: 50-70 parts of bacterial solid bacteria powder, 10-20 parts of fungus solid bacteria powder, 5-15 parts of actinomycete solid bacteria powder, 3-10 parts of protective agent and 5-10 parts of fermentation aid.
The preparation method of the environment-friendly ferment starter comprises the following steps:
A. preparing solid bacterial powder: weighing 50-100g of plant root hairs, adding the plant root hairs into a triangular flask containing 200-400mL of sterile water, shaking for 5-10 minutes to uniformly disperse flora in the sterile water to obtain microbial flora suspension, respectively inoculating 0.5-1mL of the microbial flora suspension into a beef extract peptone solid plate culture medium, culturing in an incubator at 28-30 ℃ until bacterial colonies grow out, selecting the microorganisms in the bacterial colonies, performing strain propagation by using a liquid culture medium of the beef extract peptone, inoculating the bacterial flora suspension into a peel vegetable mixed solution for liquid fermentation, performing centrifugal concentration after fermenting for 3-5 days under aerobic condition, adding resistant starch, and performing spray drying to obtain bacterial solid bacterial powder;
B. preparing actinomycete solid bacteria powder: weighing 50-100g of plant root hairs, adding the plant root hairs into a triangular flask containing 200-400mL of sterile water, shaking for 5-10 minutes to uniformly disperse flora in the sterile water to obtain microbial flora suspension, respectively inoculating 0.5-1mL of the microbial flora suspension into a starch agar solid plate culture medium, culturing in an incubator at 28-30 ℃ until bacterial colonies grow out, selecting microorganisms in the bacterial colonies, performing strain expansion culture by using a starch liquid culture medium, inoculating into a pericarp vegetable mixed solution for liquid fermentation, performing centrifugal concentration after fermenting for 3-5 days under aerobic condition, adding resistant starch, and performing spray drying to obtain actinomycete solid bacterial powder;
C. preparing solid fungus powder: weighing 50-100g of plant root hairs, adding the plant root hairs into a triangular flask containing 200-400mL of sterile water, shaking for 5-10 minutes to uniformly disperse flora in the sterile water to obtain microbial flora suspension, respectively inoculating 0.5-1mL of the microbial flora suspension into a Martin agar plate culture medium, culturing in an incubator at 28-30 ℃ until bacterial colonies grow out, selecting the microorganisms in the bacterial colonies, performing strain expansion culture by using a liquid culture medium, inoculating the bacterial colonies into fruit peel and vegetable mixed liquid for liquid fermentation, performing centrifugal concentration after fermentation for 3-5 days under aerobic condition, adding resistant starch, and performing spray drying to obtain fungus solid bacterial powder;
D. inoculating and mixed culturing: respectively activating the solid bacterial powder obtained in the steps A, B and C in water at 30 ℃ according to a formula ratio, adding the activated solid bacterial powder into a mixed culture medium, culturing in an incubator at 28-30 ℃ until bacterial colonies grow out, wherein the three bacterial colonies are mutually nutritious and have a stable state, and thus, a microbial flora of the environment-friendly ferment is obtained;
E. preparing a leavening agent by freeze drying: and D, performing expanded culture on the microbial flora obtained in the step D, adding a fermentation aid and a protective agent according to a formula ratio, and performing freeze drying to obtain the fermentation agent.
Wherein the plant root hair is the root hair of any one of reed, cattail, hyacinth, juncus effuses and eichhornia crassipes.
The fermentation auxiliary agent comprises the following components in parts by weight: 20-40 parts of white sugar, 3-5 parts of salt, 1-3 parts of sodium bicarbonate, 1-3 parts of citric acid and 40-60 parts of starch.
The mixed culture medium comprises the following components in parts by weight: 2-3 parts of beef extract, 1-3 parts of peptone, 3-7 parts of soluble starch, 3-5 parts of glucose and KH2PO40.5-2 parts of sodium chloride, 0.5-1 part of sterile water and 150 parts of sterile water.
The freeze-drying protective agent comprises the following components in parts by weight: 5-20 parts of resistant starch, 5-10 parts of trehalose, 0.2-1 part of glycerol and 0.1-0.3 part of antioxidant.
The total bacterial colony number in the bacterial solid bacterial powder prepared in the step A is 3-8 multiplied by 107cfu/g, the total number of the colonies of the actinomycetes in the actinomycetes solid bacterium powder obtained in the step B is 3-5 multiplied by 107cfu/g, the total number of fungus colonies in the fungus solid powder prepared in the step C is 3-5 multiplied by 107cfu/g。
The total number of total viable bacteria in the starter is 5-8 × 107cfu/g, wherein bacteria: (Bacteria) The viable count of (A) is 60-70% of the total viable count, and fungi (A), (B)Fungus) The viable count of (A) is 10-20% of the total viable count, and actinomycetes (A), (B), (C)Actinomycete) The number of viable bacteria is 10-20% of the total number of viable bacteria.
Compared with the prior art, three microorganism strains of bacteria, fungi and actinomycetes are screened and separated from plant rhizosphere microorganisms, then three solid bacteria powder is obtained through strain propagation, liquid state fermentation, centrifugal concentration and spray drying, the three solid bacteria powder is activated and inoculated into a mixed culture medium for propagation, after the three bacteria colonies are mutually nutritious and stable in state, after the bacteria colonies are propagated and cultured, a protective agent is added, and then freeze drying is carried out to obtain the environment-friendly ferment starter, the symbiotic problem of different strains is solved, the multiple microorganism strains are compounded, the obtained microorganism strains are mutually nutritious and stable in state, different microorganisms can have different effects in the environment-friendly ferment, and the functions of the various microorganisms are brought into full play.
The obtained environment-friendly ferment starter mainly comprises three microorganisms, wherein the functions of the microorganisms in the environment-friendly ferment are different, and the bacteria convert complex organic compounds into inorganic compounds which can be utilized by plants and microorganisms; fungi can cause the decomposition of cellulose, lignin, pectin and the like, and most of carbon sources and nutrient substances generated by the action of the fungi are utilized by plants; the actinomycetes are active participants for decomposing organic pollutants, and meanwhile, the antibiotic substances are formed to maintain the dynamic balance of wetland biological communities.
Drawings
FIG. 1 is a flow chart of a preparation process of an environment-friendly ferment starter.
Detailed Description
The present invention further describes an environmental-friendly ferment starter and a preparation method thereof with reference to the accompanying drawings and examples.
Example 1
Weighing 50g of plant root hairs, adding the plant root hairs into a triangular flask containing 200mL of sterile water, shaking for 5 minutes to uniformly disperse flora in the sterile water to obtain microbial flora suspension, respectively taking 0.5mL of the microbial flora suspension, inoculating the microbial flora suspension into a beef extract peptone solid plate culture medium, culturing the beef extract peptone solid plate culture medium and the martin agar plate culture medium in an incubator at 28 ℃ until colonies grow out, selecting microorganisms in the colonies, performing strain propagation by using corresponding liquid culture media, inoculating the beef extract peptone solid plate culture medium and the martin vegetable mixed liquid for liquid fermentation, performing centrifugal concentration after fermenting for 3 days under aerobic conditions, adding resistant starch, and performing spray drying to obtain three solid bacterial powders; respectively activating 50 parts of bacterial solid bacteria powder, 20 parts of fungus solid bacteria powder and 5 parts of actinomycete solid bacteria powder in water at 30 ℃ according to a formula ratio, adding the activated bacteria powder into a mixed culture medium, culturing the bacteria in an incubator at 28 ℃ until bacterial colonies grow out, and obtaining the microbial flora of the environment-friendly ferment, wherein the three bacterial colonies are mutually nutritious and have stable state; and (3) performing expanded culture on the obtained microbial flora, adding a protective agent 3 and 10 parts of a fermentation aid according to the formula proportion, and performing freeze drying to obtain the leavening agent.
Wherein the plant root hair is the root hair of reed.
The fermentation auxiliary agent comprises the following components in parts by weight: 20 parts of white sugar, 5 parts of salt, 1 part of sodium bicarbonate, 3 parts of citric acid and 40 parts of starch.
The mixed culture medium comprises the following components in parts by weight: 2 parts of beef extract, 3 parts of peptone, 3 parts of soluble starch, 5 parts of glucose and KH2PO40.5 part, 1 part of sodium chloride and 100 parts of sterile water.
The freeze-drying protective agent comprises the following components in parts by weight: 5 parts of resistant starch, 10 parts of trehalose, 0.2 part of glycerol and 0.3 part of antioxidant.
The total bacterial colony number in the obtained bacterial solid powder is 3 × 107cfu/g, the total number of colonies of the actinomycetes in the actinomycetes solid powder is 3 multiplied by 107cfu/g, the total number of fungus colonies in the fungus solid powder is 3 multiplied by 107cfu/g。
The total number of total viable bacteria in the starter is 5 multiplied by 107cfu/g, wherein bacteria: (Bacteria) The viable count of (b) is 60% of the total viable count, and fungiFungus) The viable cell count of (2) is 20% of the total viable cell count, and actinomycetes (A), (B), (C)Actinomycete) The number of viable bacteria of (2) is 20% of the total number of viable bacteria.
Example 2
Weighing 100g of plant root hairs, adding the plant root hairs into a triangular flask containing 400mL of sterile water, shaking for 8 minutes to uniformly disperse flora in the sterile water to obtain microbial flora suspension, respectively taking 1mL of the microbial flora suspension, inoculating the microbial flora suspension into a beef extract peptone solid plate culture medium, culturing the microbial flora suspension and a martin agar plate culture medium in an incubator at 30 ℃ until bacterial colonies grow out, selecting microorganisms in the bacterial colonies, performing strain expansion culture by using corresponding liquid culture media, inoculating the microorganisms into a peel and vegetable mixed solution for liquid fermentation, performing centrifugal concentration after fermenting for 4 days under aerobic conditions, adding resistant starch, and performing spray drying to obtain three solid bacterial powders; respectively activating 60 parts of bacterial solid bacteria powder, 15 parts of fungus solid bacteria powder and 10 parts of actinomycete solid bacteria powder in water at 30 ℃ according to a formula ratio, adding the activated bacteria powder into a mixed culture medium, culturing the bacteria powder in an incubator at 30 ℃ until bacterial colonies grow out, and obtaining the microbial flora of the environment-friendly ferment, wherein the three bacterial colonies are mutually nutritious and have stable state; and (3) performing expanded culture on the obtained microbial flora, adding 7 parts of protective agent and 8 parts of fermentation aid according to the formula proportion, and performing freeze drying to obtain the leavening agent.
Wherein the plant root hair refers to the root hair of the water hyacinth.
The fermentation auxiliary agent comprises the following components in parts by weight: 30 parts of white sugar, 4 parts of salt, 2 parts of sodium bicarbonate, 2 parts of citric acid and 50 parts of starch.
The mixed culture medium comprises the following components in parts by weight: 2.5 parts of beef extract, 2 parts of peptone, 5 parts of soluble starch, 4 parts of glucose and KH2PO41 part, 0.8 part of sodium chloride and 120 parts of sterile water.
The freeze-drying protective agent comprises the following components in parts by weight: 10 parts of resistant starch, 8 parts of trehalose, 0.6 part of glycerol and 0.2 part of antioxidant.
The total bacterial colony number in the obtained bacterial solid powder is 5 × 107cfu/g, the total number of colonies of the actinomycetes in the actinomycetes solid powder is 4 multiplied by 107cfu/g, the total number of fungus colonies in the fungus solid powder is 4 multiplied by 107cfu/g。
The total number of total viable bacteria in the starter is 6 multiplied by 107cfu/g, wherein bacteria: (Bacteria) The viable count of (2) is 65% of the total viable count, and fungi (C)Fungus) The viable cell count of (2) is 15% of the total viable cell count, and actinomycetes (A), (B), (C)Actinomycete) The number of viable bacteria of (2) is 20% of the total number of viable bacteria.
Example 3
Weighing 100g of plant root hairs, adding the plant root hairs into a triangular flask containing 400mL of sterile water, shaking for 10 minutes to uniformly disperse flora in the sterile water to obtain microbial flora suspension, respectively taking 1mL of the microbial flora suspension, inoculating the microbial flora suspension into a beef extract peptone solid plate culture medium, inoculating the microbial flora suspension into a starch agar solid plate culture medium and a martin agar plate culture medium, culturing in an incubator at 30 ℃ until bacterial colonies grow out, selecting microorganisms in the bacterial colonies, performing strain expansion culture by using corresponding liquid culture media, inoculating the bacterial colonies into a pericarp and vegetable mixed solution for liquid fermentation, performing centrifugal concentration after fermentation for 5 days under aerobic conditions, adding resistant starch, and performing spray drying to obtain three solid bacterial powders; respectively activating 70 parts of bacterial solid bacteria powder, 10 parts of fungus solid bacteria powder and 15 parts of actinomycete solid bacteria powder in water at 30 ℃ according to a formula ratio, adding the activated bacterial solid bacteria powder, the activated fungal solid bacteria powder and the actinomycete solid bacteria powder into a mixed culture medium, culturing the mixed culture medium in an incubator at 30 ℃ until bacterial colonies grow out, and obtaining microbial floras of the environment-friendly ferment, wherein the bacterial colonies are mutually nutritious and have stable states; and (3) performing expanded culture on the obtained microbial flora, adding 10 parts of protective agent and 5 parts of fermentation aid according to the formula proportion, and performing freeze drying to obtain the leavening agent.
Wherein the plant root hair refers to the root hair of juncus effuses.
The fermentation auxiliary agent comprises the following components in parts by weight: 40 parts of white sugar, 3 parts of salt, 3 parts of sodium bicarbonate, 1 part of citric acid and 60 parts of starch.
The mixed culture medium comprises the following components in parts by weight: 3 parts of beef extract, 1 part of peptone, 7 parts of soluble starch, 3 parts of glucose and KH2PO42 parts, 0.5 part of sodium chloride and 150 parts of sterile water.
The freeze-drying protective agent comprises the following components in parts by weight: 20 parts of resistant starch, 5 parts of trehalose, 0.2 part of glycerol and 0.3 part of antioxidant.
The total bacterial colony number in the obtained bacterial solid powder is 8 × 107cfu/g, the total number of colonies of the actinomycetes in the actinomycetes solid powder is 5 multiplied by 107cfu/g, the total number of fungus colonies in the fungus solid powder is 5 multiplied by 107cfu/g。
The total number of total viable bacteria in the starter is 8 multiplied by 107cfu/g, wherein bacteria: (Bacteria) The viable count of (2) is 70% of the total viable count, and fungiFungus) The viable cell count of (2) is 20% of the total viable cell count, and actinomycetes (A), (B), (C)Actinomycete) The number of viable bacteria of (2) is 10% of the total number of viable bacteria.
The above description is only a preferred embodiment of the present invention, and is not intended to limit the present invention in any way, and any simple modification, material change, equivalent change and modification made to the above embodiment according to the technical spirit of the present invention are within the scope of the technical solution of the present invention without departing from the technical solution of the present invention.
Claims (1)
1. The preparation method of the environment-friendly ferment starter is characterized in that three microorganism strains including bacteria, fungi and actinomycetes are screened and separated from plant rhizosphere microorganisms through different culture media, then three solid bacteria powders are obtained through strain propagation, liquid state fermentation, centrifugal concentration and spray drying, the three solid bacteria powders are activated and then inoculated into a mixed culture medium for culture, after the three bacterial colonies are mutually nutritious and stable in state, after the flora is propagated and cultured, a fermentation auxiliary agent and a protective agent are added, and then the mixture is freeze-dried to obtain the environment-friendly ferment starter, and the environment-friendly ferment starter is characterized by comprising the following components in parts by weight: 50-70 parts of bacterial solid bacteria powder, 10-20 parts of fungus solid bacteria powder, 5-15 parts of actinomycete solid bacteria powder, 3-10 parts of protective agent and 5-10 parts of fermentation auxiliary agent; the preparation method of the environment-friendly ferment starter comprises the following steps:
A. preparing solid bacterial powder: weighing 50-100g of plant root hairs, adding the plant root hairs into a triangular flask containing 200-400mL of sterile water, shaking for 5-10 minutes to uniformly disperse flora in the sterile water to obtain microbial flora suspension, respectively inoculating 0.5-1mL of the microbial flora suspension into a beef extract peptone solid plate culture medium, culturing in an incubator at 28-30 ℃ until bacterial colonies grow out, selecting the microorganisms in the bacterial colonies, performing strain propagation by using a liquid culture medium of the beef extract peptone, inoculating the bacterial flora suspension into a peel vegetable mixed solution for liquid fermentation, performing centrifugal concentration after fermenting for 3-5 days under aerobic condition, adding resistant starch, and performing spray drying to obtain bacterial solid bacterial powder;
B. preparing actinomycete solid bacteria powder: weighing 50-100g of plant root hairs, adding the plant root hairs into a triangular flask containing 200-400mL of sterile water, shaking for 5-10 minutes to uniformly disperse flora in the sterile water to obtain microbial flora suspension, respectively inoculating 0.5-1mL of the microbial flora suspension into a starch agar solid plate culture medium, culturing in an incubator at 28-30 ℃ until bacterial colonies grow out, selecting microorganisms in the bacterial colonies, performing strain expansion culture by using a starch liquid culture medium, inoculating into a pericarp vegetable mixed solution for liquid fermentation, performing centrifugal concentration after fermenting for 3-5 days under aerobic condition, adding resistant starch, and performing spray drying to obtain actinomycete solid bacterial powder;
C. preparing solid fungus powder: weighing 50-100g of plant root hairs, adding the plant root hairs into a triangular flask containing 200-400mL of sterile water, shaking for 5-10 minutes to uniformly disperse flora in the sterile water to obtain microbial flora suspension, respectively inoculating 0.5-1mL of the microbial flora suspension into a martin agar plate culture medium, culturing in an incubator at 28-30 ℃ until bacterial colonies grow out, selecting the microorganisms in the bacterial colonies, performing strain expansion culture by using the martin liquid culture medium, inoculating the bacterial colonies into fruit peel and vegetable mixed liquid for liquid fermentation, performing centrifugal concentration after fermenting for 3-5 days under aerobic condition, adding resistant starch, and performing spray drying to obtain fungus solid bacterial powder;
D. inoculating and mixed culturing: respectively activating the solid bacterial powder obtained in the steps A, B and C in water at 30 ℃ according to a formula ratio, adding the activated solid bacterial powder into a mixed culture medium, culturing in an incubator at 28-30 ℃ until bacterial colonies grow out, wherein the three bacterial colonies are mutually nutritious and have a stable state, and thus, a microbial flora of the environment-friendly ferment is obtained;
E. preparing a leavening agent by freeze drying: d, performing expanded culture on the microbial flora obtained in the step D, adding a fermentation aid and a protective agent according to a formula ratio, and performing freeze drying to obtain a fermentation agent;
the plant root hair is the root hair of any one of reed, water hyacinth and rush;
the fermentation auxiliary agent comprises the following components in parts by weight: 20-40 parts of white sugar, 3-5 parts of salt, 1-3 parts of sodium bicarbonate, 1-3 parts of citric acid and 40-60 parts of starch;
the mixed culture medium comprises the following components in parts by weight: 2-3 parts of beef extract, 1-3 parts of peptone, 3-7 parts of soluble starch, 3-5 parts of glucose and KH2PO40.5-2 parts of sodium chloride, 0.5-1 part of sterile water and 150 parts of sterile water;
the freeze-drying protective agent comprises the following components in parts by weight: 5-20 parts of resistant starch, 5-10 parts of trehalose, 0.2-1 part of glycerol and 0.1-0.3 part of antioxidant;
the total bacterial colony number in the bacterial solid bacterial powder prepared in the step A is 3-8 multiplied by 107cfu/g, the total number of the colonies of the actinomycetes in the actinomycetes solid bacterium powder obtained in the step B is 3-5 multiplied by 107cfu/g, the total number of fungus colonies in the fungus solid powder prepared in the step C is 3-5 multiplied by 107cfu/g；
The total viable count in the starter is 5-8 × 107cfu/g, wherein the viable count of Bacteria (Bacteria) is 60-70% of the total viable count, the viable count of fungi (Fungus) is 10-20% of the total viable count, and the viable count of actinomycetes (Actinomycete) is 10-20% of the total viable count.
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