CN107064529A - A kind of automatic analysing apparatus and automatic analysis method - Google Patents
A kind of automatic analysing apparatus and automatic analysis method Download PDFInfo
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- CN107064529A CN107064529A CN201710222206.2A CN201710222206A CN107064529A CN 107064529 A CN107064529 A CN 107064529A CN 201710222206 A CN201710222206 A CN 201710222206A CN 107064529 A CN107064529 A CN 107064529A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N35/1009—Characterised by arrangements for controlling the aspiration or dispense of liquids
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Abstract
The invention discloses a kind of automatic analysing apparatus and automatic analysis method, automatic analysing apparatus includes fluid supply unit, rotation number detecting unit, reaction vessel unit, bubble detection device and calculates analytic unit；Fluid supply unit includes shuttle, reagent container, pure water container and magnetic valve；Rotation number detecting unit includes peristaltic pump, suction hose, drain hose, the 4th magnetic valve, discharge pipe, revolution speed detecting device and motor；Reaction vessel unit includes reactive tank, agitator, inductor, positive displacement pump；Bubble detection device is connected to the 4th magnetic valve and between reactive tank；Calculating analytic unit includes injection device for calculating, composition computing device；Automatic analysis method includes：Liquid injection, revolution detection, bubble detection, liquid reactions and calculating analysis.There is provided a kind of automatic analysing apparatus and automatic analysis method, it is to avoid flexible pipe aging causes unit interval injection rate to change；The setting of bubble detection device eliminates influence of the bubble in liquid to analysis precision.
Description
Technical field
The present invention relates to automatic analysis technology field, more particularly to a kind of automatic analysing apparatus and automatic analysis method.
Background technology
In order to accurately inject micro solution, conventional chemical analysis equipment can be used when carrying out high-precision chemical analysis
To peristaltic pump, because peristaltic pump is to discharge liquid with the mode of roller squeezing tube, long-term use can extend flexible pipe aging,
Cause the discharge rate of unit interval to change, it is this change cause its be applied to quantitative analysis addition mechanism when produce mistake
Difference, so as to limit its purposes.In addition, the factor such as the pressure change of solution, contact friction, temperature change, and roller
Extruding makes the situation that bubble is produced in pipe, can all cause flexible pipe pump delivery precision unstable.
Therefore, for high-precision chemical analysis, how to ensure that the accurate quantification of solution is extremely important.In recent years, in order to
The above mentioned problem of peristaltic pump is solved, particularly flexible pipe aging causes the problem of discharge rate changes, has been developed that a kind of equipment, use
The device of constant fluid volume is converted to the discharge rate of peristaltic pump the time needed for discharge, it is then determined that efflux time, with this
The precision of injection rate needed for ensuring.However, the injection rate of this equipment is determined according to injection length, exist in a liquid
The precision of injection rate can not be ensured in the case of bubble.
The content of the invention
In view of the foregoing, the invention provides it is a kind of it is simple in construction, be easy to operation, it is reasonable in design, easy to use from
Dynamic analytical equipment and automatic analysis method.
To reach above-mentioned purpose, the technical solution adopted for the present invention to solve the technical problems is：
A kind of automatic analysing apparatus, including：Fluid supply unit, rotation number detecting unit, reaction vessel unit, bubble detection
Device and calculating analytic unit；The fluid supply unit include shuttle, reagent container, pure water container, the first magnetic valve,
Second magnetic valve and the 3rd magnetic valve；The shuttle, reagent container and pure water container are connected to first electromagnetism
The input of valve, the second magnetic valve and the 3rd magnetic valve；The rotation number detecting unit is soft including peristaltic pump, suction hose, discharge
Pipe, the 4th magnetic valve, discharge pipe, revolution speed detecting device and motor；The suction hose and drain hose are connected to institute
State suction side and the outlet side of peristaltic pump；The output end of first magnetic valve, the second magnetic valve and the 3rd magnetic valve connects respectively
It is connected on the suction hose；4th magnetic valve is three-way magnetic valve, input and the row of the 4th magnetic valve
Go out flexible pipe connection, the first output end of the 4th magnetic valve is connected with the discharge pipe；The revolution speed detecting device and institute
State peristaltic pump electrical connection；The motor is electrically connected with the revolution speed detecting device；The reaction vessel unit includes reactive tank, stirred
Mix device, inductor, positive displacement pump；The agitator is arranged on the bottom in the reactive tank, and the inductor is placed on described anti-
Answer in groove, the positive displacement pump is connected to the bottom of the reactive tank；The bubble detection device is connected to the 4th magnetic valve
The second output end and between the reactive tank；The calculating analytic unit includes injection device for calculating, composition and calculates dress
Put；The injection device for calculating is electrically connected with the revolution speed detecting device and the bubble detection device, and the composition is calculated
Device is electrically connected with the injection device for calculating and the inductor.
It is preferred that, the motor is can accurately control the mechanism of angle of rotor, and it is servomotor, with encoder straight
Flow one kind in motor or brshless DC motor.
It is preferred that, the inductor is pH inductors, oxidation-reduction potential inductor, temperature inductor, absorbance sensing
One or more in device or color reaction device etc..
It is preferred that, the calculating analytic unit is also associated with display and keyboard, for showing composition, concentration and setting
Analysis program.
It is preferred that, the quantity of the reagent container is at least one.
A kind of automatic analysis method, this method includes：
(1) liquid injects：By the pure water and the reagent container in the sample in the shuttle, the pure water container
In reagent be injected into respectively by the suction hose and the peristaltic pump in the reactive tank；
(2) revolution is detected：In the peristaltic pump conveying liquid every time, the revolution speed detecting device detects the peristaltic pump
Rotor revolution information, and be converted to revolution information electric signal；
(3) bubble is detected：The air bubble content of liquid in groove is answered described in the bubble detection device detection, and is converted to bubble
Content electric signal；
(4) liquid reactions：The liquid being injected into the reactive tank reacts under the stirring of the agitator, described
Inductor records the change of the physicochemical characteristics of liquid in the reactive tank in real time；
(5) analysis is calculated：The device for calculating that injects is by the revolution information electric signal and the air bubble content telecommunications
The amount of the being actually implanted into electric signal of the liquid after bubble removing number is drawn by logical operation；The composition computing device passes through described
The change of the amount of the being actually implanted into electric signal of the liquid after bubble removing and the physicochemical characteristics of the liquid is gone to draw sample after reaction
The composition and concentration of product.
It is preferred that, every time before liquid injection, first open the first output end of the 4th magnetic valve, close described the
Second output end of four magnetic valves, until discharging the liquid of this secondary injection in the discharge pipe, turns off the 4th electricity
First output end of magnet valve, the second output end for opening the 4th magnetic valve, carry out liquid injection.
Due to the utilization of above-mentioned technical proposal, the present invention has following beneficial effect compared with prior art：
It is different with peristaltic pump injection by same suction hose there is provided a kind of automatic analysing apparatus and automatic analysis method
Liquid, it is to avoid because flexible pipe aging causes unit interval injection rate to change, injection rate is inaccurate, impact analysis precision；Meanwhile,
Different liquids are injected by same peristaltic pump, are reduced the quantity of peristaltic pump, are reduced production cost；Bubble detection device
Setting eliminate influence of the bubble in liquid to analysis precision, improve the accuracy of analysis.
Brief description of the drawings
In order to illustrate more clearly about the embodiment of the present invention or technical scheme of the prior art, below will be to embodiment or existing
There is the accompanying drawing used required in technology description to be briefly described, it should be apparent that, drawings in the following description are only this
Some embodiments of invention, for those of ordinary skill in the art, on the premise of not paying creative work, can be with
Other accompanying drawings are obtained according to these accompanying drawings.
Fig. 1 is a kind of schematic diagram of the preferred embodiment of automatic analysing apparatus one of the invention.
Fig. 2 is the schematic diagram that a kind of automatic analysis method conversion of the invention draws electric signal.
Description of reference numerals：1 motor, 2 peristaltic pumps, 3 revolution speed detecting devices, 4 suction hoses, 5 drain hoses, the inspection of 6 bubbles
Survey device, 7 reactive tanks, 8 inductors, the magnetic valves of 9a first, the magnetic valves of 9b second, the magnetic valves of 9c the 3rd, the magnetic valves of 9d the 4th, 10
Shuttle, 11 reagent containers, 12 pure water containers, 13 computer analytic units, 13a injection device for calculating, 13b compositions are calculated
Device, 14 discharge pipes, 15 positive displacement pumps, 16 agitators, A revolution information electric signals, B air bubble content electric signals, the C amounts of being actually implanted into
Electric signal.
Embodiment
With reference to specific embodiment, present disclosure is described in further detail：
Refering to Fig. 1, a kind of automatic analysing apparatus, including：Fluid supply unit, rotation number detecting unit, reaction vessel unit,
Bubble detection device and calculating analytic unit；Fluid supply unit include shuttle 10, reagent container 11, pure water container 12,
First magnetic valve 9a, the second magnetic valve 9b and the 3rd magnetic valve 9c；Shuttle 10, reagent container 11 and pure water container 12 are distinguished
It is connected to the first magnetic valve 9a, the second magnetic valve 9b and the 3rd magnetic valve 9c input；Rotation number detecting unit includes peristaltic pump
2nd, suction hose 4, drain hose 5, the 4th magnetic valve 9d, discharge pipe 14, revolution speed detecting device 3 and motor 1；Suction hose 4
Suction side and the outlet side of peristaltic pump 2 are connected to drain hose 5；First magnetic valve 9a, the second magnetic valve 9b and the 3rd
Magnetic valve 9c output end is connected on suction hose 4；4th magnetic valve 9d is three-way magnetic valve, the 4th magnetic valve 9d's
Input is connected with drain hose 5, and the 4th magnetic valve 9d the first output end is connected with discharge pipe 14；Revolution speed detecting device 3
Electrically connected with peristaltic pump 2；Motor 1 is electrically connected with revolution speed detecting device 3；Reaction vessel unit include reactive tank 7, agitator 16,
Inductor 8, positive displacement pump 15；Agitator 16 is arranged on the bottom in reactive tank 7, and inductor 8 is placed in reactive tank 7, positive displacement pump
15 are connected to the bottom of reactive tank 7；Bubble detection device 6 is connected to the 4th magnetic valve 9d the second output end and and reactive tank 7
Between；Calculating analytic unit 13 includes injection device for calculating 13a, composition computing device 13b；Inject device for calculating 13a with
Revolution speed detecting device 3 and bubble detection device 6 are electrically connected, composition computing device 13b and injection device for calculating 13a and inductor
8 electrical connections.
A kind of automatic analysis method, including liquid injection, revolution detection, bubble detection, liquid reactions and calculating analysis etc.
Step.When carrying out sample analysis, peristaltic pump 2 is driven, in order to which quantitative injection sample only opens the first magnetic valve 9a, in this way
Conveying sample is replaced completely up to the liquid and sample that original is remained in suction hose and drain hose, opens the 4th magnetic valve 9d
The first output end, sample is flowed out from discharge pipe 14.Close the 4th magnetic valve 9d the first output end, open the 4th electricity
Magnet valve 9d the second output end starts to inject sample to the quantification of reactive tank 7.When the sample size in reactive tank 7 is very few, do not reach
During the sensing position of inductor 8, close the first magnetic valve 9a and open the 3rd magnetic valve 9c, peristaltic pump 2 holds pure water from pure water
Device 12 is delivered in reactive tank 7.In the case, it is also desirable to first pass through after discharge pipe 14 discharges pure water and be re-introduced into reaction
In groove 7.So far, the injection process of sample terminates.
A certain amount of reagent is delivered in reactive tank 7 from reagent container 11, while being stirred using agitator 16.Figure
The only one of which of reagent container 11 shown in 1, can be according to the multiple reagent containers 11 of quantity configuration of actual analysis object.Reagent injects
As the method that sample injects, the second magnetic valve 9b is first only opened, the conveying of reagent is carried out with peristaltic pump 2, elder generation is needed also exist for
It is re-introduced into after by discharge pipe 14, reagent is discharged in reactive tank 7.Reagent is injected into after reactive tank 7, inductor 8 senses
The change of the physicochemical characteristics of liquid after stirring, and it is converted into electric signal.The injection of reagent is persistently carried out, to liquid
Untill physicochemical characteristics change reaches certain value.The change of this physicochemical characteristics include pH value, oxidation-reduction potential,
Temperature, transparency, color, electrical conductivity and ion concentration etc. change, therefore inductor can use pH inductors, redox electricity
Position inductor, temperature inductor, absorbance inductor, color reaction device, electrical conductivity inductor and ion concentration inductor etc.
Constitute one or more sets combination.For example, during analysis basic sample solution, acidic liquid can be added as reagent, with pH inductors
Read the change of liquid；During analysis-reduction sample, oxidizing liquid can be added, liquid is read with oxidation-reduction potential inductor
The change of body.
In the presence of bubble detection device 6 changes the electric signal B drawn, the liquid portion correspondence of only actual discharge
Revolution electric signal be construed to effectively, using electric signal C as the liquid amount of being actually implanted into electric signal, as shown in Figure 2.Bubble detection device
6 will be converted to electric signal B in liquid containing alveolate information transmits to injection device for calculating 13a.Revolution speed detecting device is used for
The revolution of rotor in peristaltic pump is detected, the revolution information of rotor is converted into electric signal A, the corner of rotor is such as converted into electronics
Pulse signal transmission by logical operation draws reality to device for calculating 13a is injected in conjunction with the electric signal of bubble detection device
Border injection rate electric signal C.The physicochemical characteristics change of the liquid amount of being actually implanted into electric signal C and liquid passes through composition computing device
13b is associated, and calculates the composition and concentration of sample.Draw after concentration, arranged the liquid in reactive tank 7 by positive displacement pump 15
Go out.Injection device for calculating 13a and composition computing device 13b be all by computer system control, injection device for calculating 13a and
Composition computing device 13b is connected with display, keyboard, can be shown composition, concentration and be set analysis program, each device can be single
Solely operation.
Although the present embodiment has used peristaltic pump, but because sample and reagent is conveyed in same flexible pipe, even if for a long time
Influence is produced on analysis precision using will not also cause flexible pipe aging.Strength of fluid C calculation formula is as follows：
I.e.
Wherein, m is the corner of rotor in peristaltic pump when sample injects, and Vr is the volume that unit angle conveys sample, Vr*m
To inject the volume of sample, Σ p for reagent reach determine pH value when angle of rotor summation, the coefficient of concentration is f, calculating it is each into
It is E to divide corresponding coefficient.Thus formula is visible, and the volume Vr of unit angle conveying can be eliminated, and Vr is long-term in flexible pipe pumping
Slowly varying characteristic value can be produced after operation, thus formula can show that strength of fluid C will not be influenceed by Vr value changes.
And the setting of bubble detection device eliminates influence of the bubble in liquid to analysis precision, it is ensured that the accuracy of analysis.
The above embodiments merely illustrate the technical concept and features of the present invention, and its object is to allow person skilled in the art
Scholar can understand present disclosure and be carried out, and it is not intended to limit the scope of the present invention, all according to the present invention
The equivalent change or modification that Spirit Essence is made, should all cover within the scope of the present invention.
Claims (7)
1. a kind of automatic analysing apparatus, it is characterised in that including：Fluid supply unit, rotation number detecting unit, reaction vessel list
Member, bubble detection device and calculating analytic unit；
The fluid supply unit includes shuttle, reagent container, pure water container, the first magnetic valve, the second magnetic valve and the
Three magnetic valves；The shuttle, reagent container and pure water container be connected to first magnetic valve, the second magnetic valve and
The input of 3rd magnetic valve；
The rotation number detecting unit includes peristaltic pump, suction hose, drain hose, the 4th magnetic valve, discharge pipe, revolution detection
Device and motor；The suction hose and drain hose are connected to suction side and the outlet side of the peristaltic pump；Described
The output end of one magnetic valve, the second magnetic valve and the 3rd magnetic valve is connected on the suction hose；4th electromagnetism
Valve is three-way magnetic valve, and the input of the 4th magnetic valve is connected with the drain hose, the first of the 4th magnetic valve
Output end is connected with the discharge pipe；The revolution speed detecting device is electrically connected with the peristaltic pump；The motor with described turn
Number detection means electrical connection；
The reaction vessel unit includes reactive tank, agitator, inductor, positive displacement pump；The agitator is arranged on the reaction
Bottom in groove, the inductor is placed in the reactive tank, and the positive displacement pump is connected to the bottom of the reactive tank；
The bubble detection device is connected between the second output end of the 4th magnetic valve and the reactive tank；
The calculating analytic unit includes injection device for calculating, composition computing device；It is described injection device for calculating with it is described
Revolution speed detecting device and bubble detection device electrical connection, the composition computing device and the injection device for calculating and institute
State inductor electrical connection.
2. a kind of automatic analysing apparatus according to claim 1, it is characterised in that：The motor is that can accurately control to turn
The mechanism of sub- corner, it is one kind in servomotor, direct current generator with encoder or brshless DC motor.
3. a kind of automatic analysing apparatus according to claim 1, it is characterised in that：The inductor is pH inductors, oxygen
Change the one or more in reduction potential inductor, temperature inductor, absorbance inductor or color reaction device etc..
4. a kind of automatic analysing apparatus according to claim 1, it is characterised in that：The calculating analytic unit is also associated with
Display and keyboard, for showing composition, concentration and setting analysis program.
5. a kind of automatic analysing apparatus according to claim 1, it is characterised in that：The quantity of the reagent container is at least
One.
6. the automatic analysis method that a kind of automatic analysing apparatus using as described in any one of claim 1 to 5 is carried out, its feature
It is：This method includes：
(1) liquid injects：By in the pure water and the reagent container in the sample in the shuttle, the pure water container
Reagent is injected into the reactive tank by the suction hose and the peristaltic pump respectively；
(2) revolution is detected：In the peristaltic pump conveying liquid every time, the revolution speed detecting device detects turning for the peristaltic pump
The revolution information of son, and be converted to revolution information electric signal；
(3) bubble is detected：The air bubble content of liquid in groove is answered described in the bubble detection device detection, and is converted to air bubble content
Electric signal；
(4) liquid reactions：The liquid being injected into the reactive tank reacts under the stirring of the agitator, the sensing
Device records the change of the physicochemical characteristics of liquid in the reactive tank in real time；
(5) analysis is calculated：The injection device for calculating leads to the revolution information electric signal and the air bubble content electric signal
Cross the amount of the being actually implanted into electric signal that logical operation draws the liquid after bubble removing；The composition computing device is removed by described
The change of the amount of the being actually implanted into electric signal of liquid after bubble and the physicochemical characteristics of the liquid draws sample after reaction
Composition and concentration.
7. a kind of automatic analysis method according to claim 6, it is characterised in that：Every time before the liquid injection, first beat
Open the first output end of the 4th magnetic valve, the second output end of the 4th magnetic valve closed, until the discharge pipe
The interior liquid for discharging this secondary injection, turns off the first output end of the 4th magnetic valve, opens the 4th magnetic valve
Second output end, carries out liquid injection.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710222206.2A CN107064529A (en) | 2017-04-06 | 2017-04-06 | A kind of automatic analysing apparatus and automatic analysis method |
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| CN201710222206.2A CN107064529A (en) | 2017-04-06 | 2017-04-06 | A kind of automatic analysing apparatus and automatic analysis method |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110082543A (en) * | 2018-01-25 | 2019-08-02 | 深圳市新产业生物医学工程股份有限公司 | Automatic analysing apparatus and drawing liquid monitoring method |
| WO2020135618A1 (en) * | 2018-12-27 | 2020-07-02 | 深圳市帝迈生物技术有限公司 | Liquid addition apparatus, analyzer, and liquid addition method |
| CN111479620A (en) * | 2018-03-05 | 2020-07-31 | 深圳柔宇显示技术有限公司 | Material conveying equipment and material conveying method thereof |
| CN112730868A (en) * | 2020-12-26 | 2021-04-30 | 安徽皖仪科技股份有限公司 | Sample introduction system for continuous flow analyzer |
| CN115575340A (en) * | 2022-11-08 | 2023-01-06 | 杭州谱育科技发展有限公司 | Absorbance detection device and method |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2007333611A (en) * | 2006-06-16 | 2007-12-27 | Ebara Udylite Kk | Chemical analysis apparatus and chemical analysis method using same |
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2017
- 2017-04-06 CN CN201710222206.2A patent/CN107064529A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| JP2007333611A (en) * | 2006-06-16 | 2007-12-27 | Ebara Udylite Kk | Chemical analysis apparatus and chemical analysis method using same |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110082543A (en) * | 2018-01-25 | 2019-08-02 | 深圳市新产业生物医学工程股份有限公司 | Automatic analysing apparatus and drawing liquid monitoring method |
| CN110082543B (en) * | 2018-01-25 | 2023-08-08 | 深圳市新产业生物医学工程股份有限公司 | Automatic analysis device and liquid pumping monitoring method |
| CN111479620A (en) * | 2018-03-05 | 2020-07-31 | 深圳柔宇显示技术有限公司 | Material conveying equipment and material conveying method thereof |
| CN111479620B (en) * | 2018-03-05 | 2022-01-28 | 深圳柔宇显示技术有限公司 | Material conveying equipment and material conveying method thereof |
| WO2020135618A1 (en) * | 2018-12-27 | 2020-07-02 | 深圳市帝迈生物技术有限公司 | Liquid addition apparatus, analyzer, and liquid addition method |
| CN112730868A (en) * | 2020-12-26 | 2021-04-30 | 安徽皖仪科技股份有限公司 | Sample introduction system for continuous flow analyzer |
| CN112730868B (en) * | 2020-12-26 | 2023-11-07 | 安徽皖仪科技股份有限公司 | Sample injection system for continuous flow analyzer |
| CN115575340A (en) * | 2022-11-08 | 2023-01-06 | 杭州谱育科技发展有限公司 | Absorbance detection device and method |
| CN115575340B (en) * | 2022-11-08 | 2023-03-10 | 杭州谱育科技发展有限公司 | Absorbance detection device and method |
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Application publication date: 20170818 |