CN106883314A - A kind of method that pectin is extracted in the slag from lemon peel - Google Patents
A kind of method that pectin is extracted in the slag from lemon peel Download PDFInfo
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- CN106883314A CN106883314A CN201510942506.9A CN201510942506A CN106883314A CN 106883314 A CN106883314 A CN 106883314A CN 201510942506 A CN201510942506 A CN 201510942506A CN 106883314 A CN106883314 A CN 106883314A
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- pectin
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0006—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid
- C08B37/0045—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid alpha-D-Galacturonans, e.g. methyl ester of (alpha-1,4)-linked D-galacturonic acid units, i.e. pectin, or hydrolysis product of methyl ester of alpha-1,4-linked D-galacturonic acid units, i.e. pectinic acid; Derivatives thereof
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Abstract
A kind of method the invention discloses pectin is extracted in slag from lemon peel.Methods described comprises the following steps：Sorting, the enzyme that goes out, dry powder, extraction, decolouring, alcohol precipitation and drying, obtain solid pectin.The present invention provide pectin extraction method, overcome traditional handicraft largely use sour solvent the drawbacks of, gained pectin product purity is high, molecular structure destroy small, no solvent residue；The efficient recycling of lemon peel slag is realized, added value of product is substantially increased；The inventive method process is simple is efficient simultaneously, it is easy to industrialization.
Description
Technical field
The present invention relates to a kind of extracting method of pectin, and in particular to be a kind of method that pectin is extracted in slag from lemon peel.
Background technology
Pectin is a kind of polysaccharide macromolecular compound.The basis of pectin is same polygalacturonic acid (HGA55-65%) and poly- rhamnose galacturonic acid I (RG-I20-35%), also has the neutral sugars such as arabinose, rhamnose, galactolipin on its side chain.Pectin has good gelling and emulsibility, and with physiological functions such as anti-inflammatory, anticancer, anti diar rheas, extremely wide application is suffered from food, medicine, cosmetics and textile industry.
Research shows that the skin slag produced after lemon processing accounts for more than the 50% of total amount, but the utilization of lemon peel slag is not paid attention to by domestic people, so a large amount of lemon peel slag utilization rates are low, or even goes out of use.Meanwhile, lemon inherently has especially heavy lemon tart flavour, even if tart flavour is still present after lemon is destroyed, if the not processed utilization of these lemons, the mouldy fouling heart of lemon peel slag can be made by the fermentation of microorganism, is all harmful to environment, people and animals and crops.Containing abundant pectin and dietary fiber H1 in lemon peel slag, thus Devoting Major Efforts To Developing China's abundant lemon peel slag resource, skin slag utilization rate is improved, to meet domestic and international market demand.
Natural pectin is widely present in green higher plant tissue, to organizing emollescence.Pectin is a kind of important composition composition of plant cell wall, is primarily present in the primary wall and intercellular layer of cell membrane.And cell membrane is plant " calcium storehouse ", be primarily present form of the pectin in cell membrane is exactly " protopectin " together with calcium ion complexing, and protopectin is insoluble in water.The process of pectin extraction is sought to change existence form of the pectin in plant, and insoluble pectin is converted into soluble pectin.
At present, the method for pectin extraction is mainly acid system and enzyme process.The subject matter that acid system carries glue is that pectin molecule can be hydrolyzed in acid condition, and glycosidic bond fracture, molecular weight reduction, degree of gelation reduces；Substantial amounts of acid pickle is produced after carrying glue, environment is polluted；When pectin is extracted in different acid, different degrees of dissolvent residual can be also caused；In industrial production, acidity extraction can also cause the corrosion of equipment.The subject matter that enzyme process carries glue is that the price of enzyme is high, produces pectin high cost, is not easy to industrialization.
The content of the invention
For defect of the prior art, a kind of method it is an object of the invention to provide pectin is extracted in slag from lemon peel.To achieve the above object, the present invention uses following technical scheme：A kind of method that pectin is extracted in slag from lemon peel, comprises the following steps：
（1）Sorting：Fresh Lemon pericarp, pomace after selection and withdrawal fruit juice are raw material；
（2）Go out enzyme：
By the Fresh Lemon pericarp selected by step (1), the rinsing of pomace raw material clear water for several times, soluble impurity is removed；Then shred, be placed in stainless steel reaction pot, it is 80~90 DEG C of hot water to add pericarp, 2~4 times of pomace quality, temperature, soaks 20~30min, with the enzyme in go out enzyme pericarp, pomace contained by itself, it is lost activity, and prevents its decomposition to pectin；
（3）Dry powder：The enzyme thing that goes out after step (2) treatment is placed in microwave heating equipment, 300~700W of microwave power is adjusted, 3~5 minutes are processed, then 40~90 DEG C of dry lemon peel ground-slag ends；
（4）Extract：In above-mentioned dry lemon peel ground-slag end, by solid-liquid ratio（g/ml）Add the 0.4~1.0% of 10~30 times composite phosphate to extract solution, carry out the extraction of pectin, 400~600W of microwave power is processed 5~15 minutes, filtering obtains extract solution；
(5) decolourize：Activated carbon decolorizing will be added in extract solution, activated carbon is 1g with the mass volume ratio of solution:100rpm treatment 30-40min under the conditions of 100-150mL, 70-80 DEG C, filtering, filtrate decompression is concentrated into the 1/5 of original volume；
（6）Dry：Take step（5）Obtained concentrate, adds 0.5~1.5 times of 95% ethanol of weight, stands 10~30 minutes, and suction filtration, precipitation is washed several times with 75% ethanol, and centrifugation, the sediment that will be centrifugally separating to obtain is dried under the conditions of 50~60 DEG C, i.e. finished product.
Compared with prior art, the present invention has following beneficial effect：
(1) during the inventive method and sour solvent is not used, its method is small to pectin molecule structural damage, and product purity is high, no solvent residue, not etching apparatus, free from environmental pollution；
(2) microwave auxiliary extraction technology is applied to plant broken wall by the inventive method, compared with traditional heating water bath, greatly accelerates reaction rate；
（3）Broken wall uses compound phosphoric acid salt processing method, composite phosphate can improve the color of food, shape, the freshness and quality of food can be allowed to be maintained, and the demand of machining process can be allowed to be met, and composite phosphate is very important quality improver in food.
(4) the valuable reagents such as biology enzyme are not used during the inventive method, low production cost, step is simple, easily operation, it is easy to industrialization.
Specific embodiment
It is described further with embodiment below, but the present invention is not limited to these embodiments.
Embodiment 1
A kind of method that pectin is extracted in slag from lemon peel, step is as follows：
（1）Sorting：Fresh Lemon pericarp, pomace after selection and withdrawal fruit juice are raw material；
（2）Go out enzyme：
By the Fresh Lemon pericarp selected by step (1), the rinsing of pomace raw material clear water for several times, soluble impurity is removed；Then shred, be placed in stainless steel reaction pot, it is 80~90 DEG C of hot water to add pericarp, 2~4 times of pomace quality, temperature, soaks 20~30min, with the enzyme in go out enzyme pericarp, pomace contained by itself, it is lost activity, and prevents its decomposition to pectin；
（3）Dry powder：The enzyme thing that goes out after step (2) treatment is placed in microwave heating equipment, 300~700W of microwave power is adjusted, 3~5 minutes are processed, then 40~90 DEG C of dry lemon peel ground-slag ends；
（4）Extract：100g lemon peels ground-slag end is taken, adds the composite phosphate of 1000ml1.0% to extract solution, carry out the extraction of pectin, microwave power 400W is processed 15 minutes, and filtering obtains extract solution；
(5) decolourize：Will in extract solution add 10g activated carbons, 100rpm stirring, heating water bath to 70 DEG C decolourize 40 minutes, filtering, be concentrated under reduced pressure into the 1/5 of extracting liquid volume；
（6）Dry：0.5 times of 95% ethanol of weight is added in concentrate, 30 minutes are stood, suction filtration, precipitation is washed 3 times with 75% ethanol, and centrifugation, the sediment that will be centrifugally separating to obtain is dried under the conditions of 50~60 DEG C, obtains solid pectin.
Embodiment 2
A kind of method that pectin is extracted in slag from lemon peel, step is as follows：
（1）Sorting：Fresh Lemon pericarp, pomace after selection and withdrawal fruit juice are raw material；
（2）Go out enzyme：
By the Fresh Lemon pericarp selected by step (1), the rinsing of pomace raw material clear water for several times, soluble impurity is removed；Then shred, be placed in stainless steel reaction pot, it is 80~90 DEG C of hot water to add pericarp, 2~4 times of pomace quality, temperature, soaks 20~30min, with the enzyme in go out enzyme pericarp, pomace contained by itself, it is lost activity, and prevents its decomposition to pectin；
（3）Dry powder：The enzyme thing that goes out after step (2) treatment is placed in microwave heating equipment, 300~700W of microwave power is adjusted, 3~5 minutes are processed, then 40~90 DEG C of dry lemon peel ground-slag ends；
（4）Extract：100g lemon peels ground-slag end is taken, adds the composite phosphate of 3000ml0.4% to extract solution, carry out the extraction of pectin, microwave power 600W is processed 5 minutes, and filtering obtains extract solution；
(5) decolourize：Will in extract solution add 20g activated carbons, 100rpm stirring, heating water bath to 80 DEG C decolourize 30 minutes, filtering, be concentrated under reduced pressure into the 1/5 of extracting liquid volume；
（6）Dry：1.5 times of 95% ethanol of weight are added in concentrate, 10 minutes are stood, suction filtration, precipitation is washed 2 times with 75% ethanol, and centrifugation, the sediment that will be centrifugally separating to obtain is dried under the conditions of 50~60 DEG C, obtains solid pectin.
Embodiment 3
A kind of method that pectin is extracted in slag from lemon peel, step is as follows：
（1）Sorting：Fresh Lemon pericarp, pomace after selection and withdrawal fruit juice are raw material；
（2）Go out enzyme：
By the Fresh Lemon pericarp selected by step (1), the rinsing of pomace raw material clear water for several times, soluble impurity is removed；Then shred, be placed in stainless steel reaction pot, it is 80~90 DEG C of hot water to add pericarp, 2~4 times of pomace quality, temperature, soaks 20~30min, with the enzyme in go out enzyme pericarp, pomace contained by itself, it is lost activity, and prevents its decomposition to pectin；
（3）Dry powder：The enzyme thing that goes out after step (2) treatment is placed in microwave heating equipment, 300~700W of microwave power is adjusted, 3~5 minutes are processed, then 40~90 DEG C of dry lemon peel ground-slag ends；
（4）Extract：100g lemon peels ground-slag end is taken, adds the composite phosphate of 2000ml0.7% to extract solution, carry out the extraction of pectin, microwave power 500W is processed 10 minutes, and filtering obtains extract solution；
(5) decolourize：Will in extract solution add 16g activated carbons, 100rpm stirring, heating water bath to 75 DEG C decolourize 35 minutes, filtering, be concentrated under reduced pressure into the 1/5 of extracting liquid volume；
（6）Dry：1.0 times of 95% ethanol of weight are added in concentrate, 20 minutes are stood, suction filtration, precipitation is washed 3 times with 75% ethanol, and centrifugation, the sediment that will be centrifugally separating to obtain is dried under the conditions of 50~60 DEG C, obtains solid pectin.
In above-described embodiment 1-3, from lemon peel slag（Siccative）The pectin yield of extraction is 4.61~4.98%.
Claims (1)
1. a kind of method that pectin is extracted in slag from lemon peel, it is characterised in that methods described comprises the following steps：
（1）Sorting：Fresh Lemon pericarp, pomace after selection and withdrawal fruit juice are raw material；
（2）Go out enzyme：By the Fresh Lemon pericarp selected by step (1), the rinsing of pomace raw material clear water for several times, soluble impurity is removed；Then shred, be placed in stainless steel reaction pot, it is 80~90 DEG C of hot water to add pericarp, 2~4 times of pomace quality, temperature, soaks 20~30min, with the enzyme in go out enzyme pericarp, pomace contained by itself, it is lost activity, and prevents its decomposition to pectin；
（3）Dry powder：The enzyme thing that goes out after step (2) treatment is placed in microwave heating equipment, 300~700W of microwave power is adjusted, 3~5 minutes are processed, then 40~90 DEG C of dry lemon peel ground-slag ends；
（4）Extract：In above-mentioned dry lemon peel ground-slag end, by solid-liquid ratio（g/ml）Add the 0.4~1.0% of 10~30 times composite phosphate to extract solution, carry out the extraction of pectin, 400~600W of microwave power is processed 5~15 minutes, filtering obtains extract solution；
(5) decolourize：Activated carbon decolorizing will be added in extract solution, activated carbon is 1g with the mass volume ratio of solution:100rpm treatment 30-40min under the conditions of 100-150mL, 70-80 DEG C, filtering, filtrate decompression is concentrated into the 1/5 of original volume；
（6）Dry：Take step（5）Obtained concentrate, adds 0.5~1.5 times the 95% of weight
Ethanol, stands 10~30 minutes, and suction filtration, precipitation is washed several times with 75% ethanol, and centrifugation, the sediment that will be centrifugally separating to obtain is dried under the conditions of 50~60 DEG C, i.e. finished product.
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| CN201510942506.9A CN106883314A (en) | 2015-12-16 | 2015-12-16 | A kind of method that pectin is extracted in the slag from lemon peel |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109111532A (en) * | 2018-09-30 | 2019-01-01 | 三门峡源丰生物科技有限公司 | A kind of pectin extraction method |
| CN109438588A (en) * | 2019-01-09 | 2019-03-08 | 广州市莱檬生物科技有限公司 | A method of pectin from lemon peel is extracted by salt |
| CN113016971A (en) * | 2019-12-09 | 2021-06-25 | 南京宿根花卉植物园有限公司 | Solid beverage for reducing blood pressure |
-
2015
- 2015-12-16 CN CN201510942506.9A patent/CN106883314A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109111532A (en) * | 2018-09-30 | 2019-01-01 | 三门峡源丰生物科技有限公司 | A kind of pectin extraction method |
| CN109111532B (en) * | 2018-09-30 | 2020-07-14 | 三门峡源丰果业有限公司 | Pectin extraction method |
| CN109438588A (en) * | 2019-01-09 | 2019-03-08 | 广州市莱檬生物科技有限公司 | A method of pectin from lemon peel is extracted by salt |
| CN109438588B (en) * | 2019-01-09 | 2020-08-28 | 广州市莱檬生物科技有限公司 | Method for extracting lemon peel pectin through salt |
| CN113016971A (en) * | 2019-12-09 | 2021-06-25 | 南京宿根花卉植物园有限公司 | Solid beverage for reducing blood pressure |
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Application publication date: 20170623 |
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