CN106852256A - A kind of composite cultivation method of black fungus - Google Patents
A kind of composite cultivation method of black fungus Download PDFInfo
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- CN106852256A CN106852256A CN201611255358.4A CN201611255358A CN106852256A CN 106852256 A CN106852256 A CN 106852256A CN 201611255358 A CN201611255358 A CN 201611255358A CN 106852256 A CN106852256 A CN 106852256A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B17/00—Other phosphatic fertilisers, e.g. soft rock phosphates, bone meal
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C11/00—Other nitrogenous fertilisers
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
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Abstract
The invention provides a kind of composite cultivation method of black fungus, including carry out successively prepare liquid culture medium, after packing, sterilizing cooling, inoculate culture, obtain secondary strain；Then secondary strain is seeded on bulk solid state rheology base-material, then is cultivated, harvested.The present invention combines the liquid culture of early stage and the solid medium culture in later stage, combines the advantage of liquid culture and solid state rheology, improves the quality of black fungus, and yield is high.The black fungus that early stage liquid culture is obtained, mycelium pellet quantity is more, and small and dense reality, dry mycelial weight reaches 11.6g/L, while rich in various trace elements such as iron, zinc, selenium, iodine, magnesium.After being seeded to solid state rheology base-material in bulk, continue to cultivate growth, every square metre can be reached monthly using 1500 grams of fresh agarics, and fresh agaric color and luster is dark brown, soft texture is in colloidal substance, thin and flexible.
Description
Technical field
The present invention relates to fungus growing technique field, more particularly to a kind of black fungus composite cultivation method.
Background technology
Black fungus (Auricularia auricula) belongs to Eumycota, Basidiomycetes, Auriculariale, Auriculariaceae, Auricularia,
It is a kind of famous dietotherapeutic macro fungi, is made up of mycelium and fructification.Auricularia polysaccharide be agaric chief active into
Point, with multiple biological activities such as antitumor, antithrombotic, anti-aging, hypoglycemic, reducing blood lipid and regulation immunologic functions.Additionally, black
Iron content in agaric is that content is most abundant in various meat and vegetable foods, and edible black fungus can in time for human body supplements enough iron
Matter.Mycelium is the nutrition organs of black fungus, and root, stem equivalent to high green plants, leaf are the main bodys of black fungus.Black wood
The fructification of ear, is that we cook edible part also known as monospermous fruit.
The composite cultivation method of current black fungus has formed fixed pattern, will wood chip, wheat bran, cotton seed skin, crops
Powder of straw minces, mineral matter etc. is fitted into special high-temperature resistance plastice bag, by high temperature sterilization, inoculation, culture, scarfing produce agaric,
During produce agaric, or by produce agaric bag hanging in canopy room, or produce agaric bag setting is put on the ground, but extensive cultivation is generally deposited
Yielding poorly, poor quality, pollution rate is high, the problems such as yield is unstable, it has also become bag plants the bottleneck of agaric development, urgent need is solved
Certainly and improve.
The content of the invention
For the drawbacks described above and problem of prior art, it is an object of the invention to provide a kind of composite cultivation side of black fungus
Method.The liquid culture of early stage and the solid medium culture in later stage are combined, liquid culture and solid state rheology is combined
Advantage, improves the quality of black fungus, and yield is high.
In order to achieve the above object, the present invention provides following technical scheme：
A kind of composite cultivation method of black fungus, it is characterised in that comprise the following steps：
Step one, prepare liquid culture medium：Added in per 1L water 10g~15g glucose, 10g~15g amino acid, 5g~
10g maltose, 5g~8g dusty yeasts, 5g~8g ironic citrates, 2~4g zinc sulfate, 15~30mg vitamin B1s and 200~
The nutrient solution of 300mL；The nutrient solution is by including the stem of noble dendrobium, lily, mulberries, rhizoma imperatae, sealwort and dandelion
The Decoction of mixed material；
Step 2, packing：The liquid culture medium that step one is obtained is dispensed into triangular flask, bottle stopper is covered, on the bottle stopper
A gas port is opened up, and soft air-guide pipe is connected on the gas port, obtain triangle inoculation bottle；
Step 3, sterilizing：(1) high-temperature sterilization tank is prepared, the high-temperature sterilization tank increased multiple branch air entraining pipes and always draw
Tracheae, the inlet end of multiple branch air entraining pipes is stretched into the high-temperature sterilization tank, and outlet side is connected with total air entraining pipe；Total bleed
Connecting valve on pipe；There is the rack for placing triangle blake bottle in high-temperature sterilization tank；
(2) multiple triangle blake bottles are placed on rack, then train the inlet end of multiple branch air entraining pipes with triangle respectively
Support the soft air-guide pipe connection on the bottle stopper of bottle；Then high-temperature sterilization tank is sealed, starts to carry out to being filled with high temperature and high pressure gas in tank
Sterilizing；Sterilization process is as follows：It it is 100~110 DEG C in temperature, during pressure is the steam of 0.2~0.3MPa, sterilize 50~60min；
Then the valve on total air entraining pipe is opened, the gas in triangle blake bottle flows out under differential pressure, when being flowed out without gas, shutoff valve
Door；Then it is 120 DEG C in temperature, during pressure is the steam of 0.5~0.8MPa, sterilize 60~80min；Then total bleed is opened again
Valve on pipe, the gas in triangle blake bottle flows out under differential pressure, when being flowed out without gas, closes valve；Then proceed to go out
10~20min of bacterium, completes sterilizing；
Step 4, cooling：Then move in cooling chamber, the soft air-guide pipe sealing on the bottle stopper of triangle blake bottle three
Cool water shower device is installed in the top of the cooling position of angle blake bottle, carries out water-cooled, is cooled to 20~22 DEG C；
Step 5, inoculation：The one-level agaric parent species after activation will be purified, be linked into the sterilizing after the cooling that step 4 is obtained
In culture medium, inoculated and cultured bottle is obtained；Inoculation condition is：Under aseptic condition, temperature be 16~18 DEG C, relative humidity be 70%~
80%；
Step 6, culture：Inoculated and cultured bottle concussion and cultivate in shaking table that step 5 is obtained, obtains two grades of bacterium after culture
Kind；Concussion and cultivate condition is：Under aseptic condition, in the rotating speed of 100~120rpm, temperature is 25~26 DEG C, and relative humidity is
30%~40%, cultivate 2~3 days；
Step 7, secondary inoculation：The second class inoculum that step 6 is obtained is seeded on bulk solid state rheology base-material, is mixed,
Then pack, cultivated, to harvesting.
Further, in step one, in the nutrient solution, in mixed material, the stem of noble dendrobium, lily, mulberries, rhizoma imperatae,
The mixed proportion of sealwort and dandelion is the ﹕ 0.2~0.3 of 1 ﹕, 2~3 ﹕, 0.5~0.8 ﹕, 0.3~0.6 ﹕ 0.2~0.3；Decocting was extracted
Journey is as follows：Every time to 3~6 times of water of amount are added in mixed material, 0.5-2h is decocted, filtering obtains 1~1.5 times of decoction of amount
Liquid；Repeatedly decoct, then merge decoction liquor, obtain Chinese medicine extract.
Further, in step 4, the cool water shower device includes that water inlet main pipeline, ring-type outlet pipe and multiple are straight
Multiple apopores are opened up respectively on pipe, the ring-type outlet pipe and each straight tube；The multiple straight tube is with ring-type outlet pipe
Heart position is starting point, radially being radially arranged along the ring-type outlet pipe, and the multiple straight tube is by radiating distal end
Port connects with the ring-type outlet pipe；The multiple straight tube radiation near-end port with it is described water inlet main pipeline water outlet
Mouth connection, cooling water is flow in ring-type outlet pipe by water inlet main pipeline via multiple straight tubes, and is sprayed to triangle via apopore
On blake bottle；The mode that opens up of the apopore is：Point is in the arrangement of concentric circles centered on the center of ring-type outlet pipe
Form is opened up.
Further, in step 6, during concussion and cultivate, using every 2~3 hours discontinuous of concussion 1~2 hour
Concussion and cultivate mode.
Further preferred technical scheme is that the operating process of step 7 is as follows：A () prepares bulk solid state rheology base-material：
By weight, following raw material is weighed：20~30 parts of pig bone particles, 10~15 parts of fermented stalk particles, 20~30 parts of wood shavingses, 15
~25 parts of wheat brans, 10~15 powder maize flours, 5~10 parts of bean cake powders, 5~10 parts of precipitated calcium carbonates and 1~3 part of oyster shell whiting；Wherein,
Pig bone particle is by by the pyrolysis pig bone particle after high temperature sintering；The fermented stalk particle is will to remove sclerderm using EM bacterium solutions
Obtained after maize straw flesh fermentation process；The wood shavingses are by after limewash impregnation process；Above-mentioned raw materials are mixed
Uniformly, bulk solid state rheology base-material is obtained；The moisture content for controlling culture base-material is 55%~60%；
B bulk solid state rheology base-material that () obtains to step (a) sterilizes, after cooling；Two grades that step 6 is obtained
Strain is seeded on the bulk solid state rheology base-material after the sterilizing cooling treatment, and vibration is mixed；Then pack, obtain packed inoculation
Culture base-material；
C () moves into culture room packed inoculated and cultured base-material, condition of culture is：Temperature is 18~20 DEG C, relative humidity
It is 40%~50%, diffuses naturally irradiation 6~8 hours daily, and it is regular to spraying step on packed inoculated and cultured base-material
Liquid culture medium in one；Until harvesting.
Further, in (a) step of step 7, the preparation method of pig bone particle is as follows：By pig bone 800~1000
At a temperature of DEG C, 40~60min is calcined；The pig bone particle of 3~5mm is broken for after cooling；
The preparation method of fermented stalk particle is as follows：After fresh corn stalk is gone into sclerderm, it is 8 that maize straw flesh is crushed
After the particle of~10mm, using EM bacterium solution fermentation process, fermented stalk particle is obtained；
The preparation method of the wood shavingses is as follows：By the limewash that mass concentration is 5%~10%, planing wood is uniformly sprayed at
Take, then stack wood shavingses 7 days, obtain；
During raw material is well mixed, after first mixing fermented stalk particle, pig bone particle and wood shavingses, 5 are stacked
~7 days, obtain support aggregate；Then again by wheat bran, maize flour, bean cake powder, precipitated calcium carbonate and oyster shell whiting add support aggregate in,
Stir and evenly mix, obtain bulk culture base-material.
Further, the specification of the packed inoculated and cultured base-material is：Highly it is 20 centimetres~25 centimetres, weight in wet base 1.5 thousand
Gram~1.8 kilograms, water content 61%~63%.
The composite cultivation method of black fungus of the invention, by the liquid culture of early stage and the solid medium culture knot in later stage
Altogether, the advantage of liquid culture and solid state rheology is combined, the quality of black fungus is improve, yield is high.Early stage liquid culture
The black fungus for obtaining, mycelium pellet quantity is more, and small and dense reality, dry mycelial weight reaches 11.6g/L, while being rich in iron, zinc, selenium, iodine, magnesium
Etc. various trace elements.After being seeded to solid state rheology base-material in bulk, continue to cultivate growth, every square metre can be reached monthly
Using 1500 grams of fresh agarics, and fresh agaric color and luster is dark brown, and soft texture is in colloidal substance, thin and flexible.
And, the sterilization time in early stage liquid culture of the invention is short, reduces the time of preparation work before inoculation；And
And, high-temperature steam is not passed through in liquid culture medium, reduce influence of the steam liquefied to liquid culture medium concentration.Spray simultaneously
Cooling device water outlet is uniform, and blake bottle obtains sufficiently quick cooling, and being watered than conventional single water pipe, cool time, to shorten 8 small
When more than, largely saving energy consumption and water can not only be used, shorten the whole process time of strain making, reduce dirt
The risk of dye, and good cooling effect can be obtained.
In the composite cultivation method of black fungus of the invention, while proposing the liquid culture medium material and solid-state for using cooperatively
The formula of culture base-material, it is nutritious in culture base-material, and be all fermented and be processed as being easy to absorbed small organic molecule.And
And the addition of the support aggregate in solid state rheology base-material, increased the gas permeability of follow-up packed inoculated and cultured base-material so that black wood
Unfamiliar to the ear length is homogeneous, in the same size, and quality is excellent.
Brief description of the drawings
In order to illustrate more clearly about the embodiment of the present invention or technical scheme of the prior art, below will be to embodiment or existing
The accompanying drawing to be used needed for having technology description is briefly described, it should be apparent that, drawings in the following description are only this
Some embodiments of invention, for those of ordinary skill in the art, without having to pay creative labor, may be used also
Other accompanying drawings are obtained with according to these accompanying drawings.
Fig. 1 is a kind of FB(flow block) of the composite cultivation method of black fungus of the invention；
Fig. 2 is a kind of device structure connection diagram of the sterilization process of the composite cultivation method of black fungus of the invention；
Fig. 3 be a kind of composite cultivation method of black fungus of the invention liquid culture medium material cooling procedure in the spray that uses
Drench the structural representation of cooling device；
Fig. 4 is the present invention looks up structural representation of the spray cooling device of Fig. 3.
Specific embodiment
Below in conjunction with embodiments of the invention, technical scheme is clearly and completely described, it is clear that
Described embodiment is only a part of embodiment of the invention, rather than whole embodiments.Based on the implementation in the present invention
Example, the every other embodiment that those of ordinary skill in the art are obtained under the premise of creative work is not made is belonged to
The scope of protection of the invention.
According to Fig. 1 to Fig. 4, a kind of composite cultivation method of black fungus of the invention is illustrated, comprised the following steps：
Step one, prepare liquid culture medium：Added in per 1L water 10g~15g glucose, 10g~15g amino acid, 5g~
10g maltose, 5g~8g dusty yeasts, 5g~8g ironic citrates, 2~4g zinc sulfate, 15~30mg vitamin B1s and 200~
The nutrient solution of 300mL；The nutrient solution is by including the stem of noble dendrobium, lily, mulberries, rhizoma imperatae, sealwort and dandelion
The Decoction of mixed material；Wherein, the mixed proportion of the stem of noble dendrobium, lily, mulberries, rhizoma imperatae, sealwort and dandelion be 1 ﹕ 2~
The ﹕ 0.2~0.3 of 3 ﹕, 0.5~0.8 ﹕, 0.3~0.6 ﹕ 0.2~0.3；Decocting extraction process is as follows：Added in mixed material every time
3~6 times of water of amount, decoct 0.5-2h, and filtering obtains 1~1.5 times of decoction liquor of amount；Repeatedly decoct, then merge decoction liquor,
Obtain Chinese medicine extract.Liquid nutrient solution is nutritious, contains abundant nitrogen source, carbon source, and inorganic elements.
Step 2, packing：The liquid culture medium that step one is obtained is dispensed into triangular flask 10, bottle stopper 11 is covered, the bottle
A gas port 111 is opened up beyond the Great Wall, and soft air-guide pipe 12 is connected on the gas port 111, obtain triangle inoculation bottle.
Step 3, sterilizing：(1) high-temperature sterilization tank 20 is prepared, the high-temperature sterilization tank 20 increased multiple branch air entraining pipes 31
With total air entraining pipe 32, the inlet end of multiple branch air entraining pipes 31 stretched into the high-temperature sterilization tank 20, outlet side and total air entraining pipe 31
Connection；Connecting valve 33 on total air entraining pipe 31；There is the rack 21 for placing triangle blake bottle 10 in high-temperature sterilization tank 20.
(2) multiple triangle blake bottles 10 are placed on rack 21, then by the inlet end of multiple branch air entraining pipes 31 respectively with
Soft air-guide pipe 12 on the bottle stopper 11 of triangle blake bottle 10 is connected；Then high-temperature sterilization tank is sealed, is started to being filled with high temperature in tank
Gases at high pressure are sterilized；Sterilization process is as follows：It is 100~110 DEG C in temperature, during pressure is the steam of 0.2~0.3MPa, goes out
50~60min of bacterium；Then the valve on total air entraining pipe is opened, the gas in triangle blake bottle flows out under differential pressure, to without gas
During outflow, valve is closed；Then it is 120 DEG C in temperature, during pressure is the steam of 0.5~0.8MPa, sterilize 60~80min；So
Open the valve on total air entraining pipe again afterwards, the gas in triangle blake bottle flows out under differential pressure, when being flowed out without gas, shutoff valve
Door；10~20min of sterilizing is then proceeded to, sterilizing is completed.It is there will be to be led with the cold air in blake bottle to first switch on valve
Go out so that the pressure and temperature in seeding tank rapidly reaches sterilizing standard, effectively the liquid training to seeding tank and in it
Foster base is sterilized；After second is opened valve, after the steam outflow in blake bottle, the energy of the high temperature and high pressure steam in sterilization tank
Amount dynamically enters blake bottle again, improves sterilization effect, while sterilization time is reduced, for up to by 2.5 hours, you can complete
Sterilizing.
Step 4, cooling：Soft air-guide pipe 12 on the bottle stopper 11 of triangle blake bottle 10 is sealed, is clamped using clip sealing
.Then move in cooling chamber, cool water shower device be installed in the top of the cooling position of triangle blake bottle, carry out water-cooled,
It is cooled to 20~22 DEG C.
Step 5, inoculation：The one-level agaric parent species after activation will be purified, be linked into the sterilizing after the cooling that step 4 is obtained
In culture medium, inoculated and cultured bottle is obtained；Inoculation condition is：Under aseptic condition, temperature be 16~18 DEG C, relative humidity be 70%~
80%；
Step 6, culture：Inoculated and cultured bottle concussion and cultivate in shaking table that step 5 is obtained, obtains two grades of bacterium after culture
Kind；Concussion and cultivate condition is：Under aseptic condition, in the rotating speed of 150rpm, temperature is 25~26 DEG C, relative humidity is 30%~
40%, cultivate 2~3 days；
Step 7, secondary inoculation：The second class inoculum that step 6 is obtained is seeded on bulk solid state rheology base-material, is mixed,
Then pack, cultivated, to harvesting.
In step four in above-mentioned composite cultivation method, as shown in Figure 3 and Figure 4, the cool water shower device 40 is wrapped
Include into water main pipeline 41, ring-type outlet pipe 42 and multiple straight tubes 43, opened respectively on the ring-type outlet pipe 42 and each straight tube 43
If multiple apopores 44；The multiple straight tube 43 with the center of ring-type outlet pipe 42 as starting point, radially along the ring
Being radially arranged of shape outlet pipe 42 (being radially the diametric(al) of ring-type at this), and the multiple straight tube 43 is by radiating distal end
431 port connects with the ring-type outlet pipe 42；The multiple straight tube 43 radiation near-end port 432 with the water inlet
The delivery port connection of main pipeline 41, cooling water is flow in ring-type outlet pipe 42 by water inlet main pipeline 41 via multiple straight tubes 43, and
It is sprayed on triangle blake bottle 10 via apopore 44；The mode that opens up of the apopore 44 is：With in ring-type outlet pipe 42
Point is opened up in the spread pattern of concentric circles centered on heart position.The delivery port of the water inlet main pipeline 41 and the spoke of multiple straight tubes 43
The multiple-pass joint 45 that connects through for penetrating the port 432 of near-end realizes connection.Multiple-pass joint 45 has N-1 horizontal joint 451 and 1
Individual vertical joint 452, the N-1 horizontal joint 451 in same level, vertical joint 452 and the horizontal plane；
Above-mentioned vertical joint 452 with water inlet main pipeline 41 delivery port be connected, above-mentioned N-1 horizontal joint 451 respectively correspondingly with often
The port 432 of the radiation near-end of individual straight tube 43 connects；Wherein, N >=3, and round numbers.Three straight tubes 43 are used in the present invention, it is many
Pass joint 45 uses four-way connection；And ring-type outlet pipe 42 and straight tube 43 are stainless steel.
In the cultural method of black fungus of the invention, in step 6, during concussion and cultivate, shaken using every 2~3 hours
Swing the discontinuous concussion and cultivate mode of 1~2 hour.Concussion state need not be constantly in, in nutrient solution in the quiescent state, strain
Growth is more preferable.
In the cultural method of black fungus of the invention, answering for the culture of early stage liquid and later stage solid medium culture is employed
Conjunction mode, combines both advantages.Therefore, the present invention in further preferably give step its secondary inoculation solid medium and
The operational means of its follow-up cultivation.It is specific as follows：
The operating process of step 7 is as follows：A () prepares bulk solid state rheology base-material：By weight, following raw material is weighed：20
~30 parts of pig bone particles, 10~15 parts of fermented stalk particles, 20~30 parts of wood shavingses, 15~25 parts of wheat brans, 10~15 powder corns
Face, 5~10 parts of bean cake powders, 5~10 parts of precipitated calcium carbonates and 1~3 part of oyster shell whiting；Wherein, the preparation method of pig bone particle is as follows：
By pig bone at a temperature of 800~1000 DEG C, 40~60min is calcined；The pig bone particle of 3~5mm is broken for after cooling；Fermentation
The preparation method of stalk particle is as follows：After fresh corn stalk is gone into sclerderm, maize straw flesh is crushed into the particle for 8~10mm
Afterwards, using EM bacterium solution fermentation process, fermented stalk particle is obtained；The preparation method of the wood shavingses is as follows：It is by mass concentration
5%~10% limewash, is uniformly sprayed on wood shavingses, then stacks wood shavingses 7 days, obtains.Then by fermented stalk
After the mixing of particle, pig bone particle and wood shavingses, stack 5~7 days, obtain support aggregate；Then again by wheat bran, maize flour, bean cake powder,
Precipitated calcium carbonate and oyster shell whiting are added in support aggregate, are stirred and evenly mixed, and are then pressed into type, obtain bar-shaped culture base-material.Control
The moisture content of bulk culture base-material is 55%~60%.
B bulk solid state rheology base-material that () obtains to step (a) uses existing conventional sterilant mode, after cooling；By step
Six second class inoculums for obtaining are seeded on the bulk solid state rheology base-material after the sterilizing cooling treatment, and vibration is mixed；Then dress modeling
Pocket, obtains packed inoculated and cultured base-material；The specification for controlling packed inoculated and cultured base-material is：Highly it is 20 centimetres~25 centimetres,
1.5 kilograms~1.8 kilograms of weight in wet base, water content 61%~63%.
C () moves into culture room packed inoculated and cultured base-material, condition of culture is：Temperature is 18~20 DEG C, relative humidity
It is 40%~50%, diffuses naturally irradiation 6~8 hours daily, and it is regular to spraying step on bag-shaped inoculated and cultured base-material
Liquid culture medium in one；Until harvesting.
The above, specific embodiment only of the invention, but protection scope of the present invention is not limited thereto, and it is any
Those familiar with the art the invention discloses technical scope in, change or replacement can be readily occurred in, should all contain
Cover within protection scope of the present invention.Therefore, protection scope of the present invention described should be defined by scope of the claims.
Claims (7)
1. a kind of composite cultivation method of black fungus, it is characterised in that comprise the following steps：
Step one, prepare liquid culture medium：10g~15g glucose, 10g~15g amino acid, 5g~10g wheats are added in per 1L water
The battalion of bud sugar, 5g~8g dusty yeasts, 5g~8g ironic citrates, 2~4g zinc sulfate, 15~30mg vitamin B1s and 200~300mL
Support annex solution；The nutrient solution is by the mixed material including the stem of noble dendrobium, lily, mulberries, rhizoma imperatae, sealwort and dandelion
Decoction；
Step 2, packing：The liquid culture medium that step one is obtained is dispensed into triangular flask, bottle stopper is covered, opened up on the bottle stopper
One gas port, and soft air-guide pipe is connected on the gas port, obtain triangle inoculation bottle；
Step 3, sterilizing：(1) high-temperature sterilization tank is prepared, the high-temperature sterilization tank increased multiple branch air entraining pipes and total air entraining pipe,
The inlet end of multiple branch air entraining pipes is stretched into the high-temperature sterilization tank, and outlet side is connected with total air entraining pipe；On total air entraining pipe
Connecting valve；There is the rack for placing triangle blake bottle in high-temperature sterilization tank；
(2) multiple triangle blake bottles are placed on rack, then by the inlet end of multiple branch air entraining pipes respectively with triangle blake bottle
Bottle stopper on soft air-guide pipe connection；Then high-temperature sterilization tank is sealed, starts to be sterilized to being filled with high temperature and high pressure gas in tank；
Sterilization process is as follows：It it is 100~110 DEG C in temperature, during pressure is the steam of 0.2~0.3MPa, sterilize 50~60min；Then
The valve on total air entraining pipe is opened, the gas in triangle blake bottle flows out under differential pressure, when being flowed out without gas, close valve；
Then it is 120 DEG C in temperature, during pressure is the steam of 0.5~0.8MPa, sterilize 60~80min；Then total air entraining pipe is opened again
On valve, the gas in triangle blake bottle flows out under differential pressure, when flow out without gas, closing valve；Then proceed to sterilizing
10~20min, completes sterilizing；
Step 4, cooling：By the soft air-guide pipe sealing on the bottle stopper of triangle blake bottle, then move in cooling chamber, in triangle training
Cool water shower device is installed in the top for supporting the cooling position of bottle, carries out water-cooled, is cooled to 20~22 DEG C；
Step 5, inoculation：The one-level agaric parent species after activation will be purified, be linked into the sterilizing culture after the cooling that step 4 is obtained
In base, inoculated and cultured bottle is obtained；Inoculation condition is：Under aseptic condition, temperature be 16~18 DEG C, relative humidity be 70%~
80%；
Step 6, culture：Inoculated and cultured bottle concussion and cultivate in shaking table that step 5 is obtained, obtains second class inoculum after culture；
Concussion and cultivate condition is：Under aseptic condition, in the rotating speed of 100~120rpm, temperature is 25~26 DEG C, relative humidity is 30%~
40%, cultivate 2~3 days；
Step 7, secondary inoculation：The second class inoculum that step 6 is obtained is seeded on bulk solid state rheology base-material, is mixed, then
Pack, is cultivated, to harvesting.
2. the composite cultivation method of a kind of black fungus according to claim 1, it is characterised in that in step one, the battalion
Support in annex solution, in mixed material, the mixed proportion of the stem of noble dendrobium, lily, mulberries, rhizoma imperatae, sealwort and dandelion is the ﹕ of 1 ﹕ 2~3
The ﹕ 0.2~0.3 of 0.5~0.8 ﹕, 0.3~0.6 ﹕ 0.2~0.3；Decocting extraction process is as follows：Every time to adding 3 in mixed material
~6 times of water of amount, decoct 0.5-2h, and filtering obtains 1~1.5 times of decoction liquor of amount；Repeatedly decoct, then merge decoction liquor, obtain
To Chinese medicine extract.
3. the composite cultivation method of a kind of black fungus according to claim 1, it is characterised in that described cold in step 4
But water spray system includes water inlet main pipeline, ring-type outlet pipe and multiple straight tubes, divides on the ring-type outlet pipe and each straight tube
Multiple apopores are not opened up；The multiple straight tube with the center of ring-type outlet pipe as starting point, radially along the ring-type
Outlet pipe is radially arranged, and the multiple straight tube connects by radiating the port of distal end with the ring-type outlet pipe；It is described many
Delivery port of the port of the radiation near-end of individual straight tube with the water inlet main pipeline is connected, and cooling water is by water inlet main pipeline via many
Individual straight tube is flow in ring-type outlet pipe, and is sprayed on triangle blake bottle via apopore；The mode that opens up of the apopore is：
Point is opened up in the spread pattern of concentric circles centered on the center of ring-type outlet pipe.
4. the composite cultivation method of a kind of black fungus according to claim 3, it is characterised in that in step 6, concussion training
During supporting, using every 2~3 hours discontinuous concussion and cultivate modes of concussion 1~2 hour.
5. a kind of composite cultivation method of black fungus according to any one of claim 1 to 4, it is characterised in that step
Seven operating process is as follows：A () prepares bulk solid state rheology base-material：By weight, following raw material is weighed：20~30 parts of pig bones
Grain, 10~15 parts of fermented stalk particles, 20~30 parts of wood shavingses, 15~25 parts of wheat brans, 10~15 powder maize flours, 5~10 parts of beans
Dregs of rice powder, 5~10 parts of precipitated calcium carbonates and 1~3 part of oyster shell whiting；Wherein, pig bone particle is by by the pyrolysis pig bone after high temperature sintering
Particle；The fermented stalk particle is obtained after the maize straw flesh fermentation process that sclerderm will be removed using EM bacterium solutions；The plane
Wooden flower is by after limewash impregnation process；Above-mentioned raw materials are well mixed, bulk solid state rheology base-material is obtained；Control culture
The moisture content of base-material is 55%~60%；
B bulk solid state rheology base-material that () obtains to step (a) sterilizes, after cooling；The second class inoculum that step 6 is obtained
It is seeded on the bulk solid state rheology base-material after the sterilizing cooling treatment, vibration is mixed；Then pack, obtain packed inoculated and cultured
Base-material；
C () moves into culture room packed inoculated and cultured base-material, condition of culture is：Temperature is 18~20 DEG C, and relative humidity is
40%~50%, diffuse irradiation 6~8 hours naturally daily, and periodically to sprinkling step one on packed inoculated and cultured base-material
In liquid culture medium；Until harvesting.
6. a kind of composite cultivation method of black fungus according to claim 5, it is characterised in that (a) step of step 7
In, the preparation method of pig bone particle is as follows：By pig bone at a temperature of 800~1000 DEG C, 40~60min is calcined；After cooling
It is broken for the pig bone particle of 3~5mm；
The preparation method of fermented stalk particle is as follows：After fresh corn stalk is gone into sclerderm, maize straw flesh is crushed as 8~
After the particle of 10mm, using EM bacterium solution fermentation process, fermented stalk particle is obtained；
The preparation method of the wood shavingses is as follows：By the limewash that mass concentration is 5%~10%, wood shavingses are uniformly sprayed at
On, then wood shavingses are stacked 7 days, obtain；
During raw material is well mixed, after first mixing fermented stalk particle, pig bone particle and wood shavingses, 5~7 are stacked
My god, obtain support aggregate；Then stirred in wheat bran, maize flour, bean cake powder, precipitated calcium carbonate and oyster shell whiting addition support aggregate again
Mixing is mixed, bulk culture base-material is obtained.
7. a kind of composite cultivation method of black fungus according to claim 5, it is characterised in that the packed inoculated and cultured
The specification of base-material is：Highly it is 20 centimetres~25 centimetres, 1.5 kilograms~1.8 kilograms of weight in wet base, water content 61%~63%.
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| CN107711287A (en) * | 2017-10-26 | 2018-02-23 | 日照市经济作物站 | It is a kind of to produce the method for black fungus and quantitative classification inoculation apparatus with liquid spawn |
| CN108293593A (en) * | 2017-10-17 | 2018-07-20 | 辽宁天赢生物科技股份有限公司 | A method of utilizing corncob mixture cultivating black fungus |
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