CN106855515B - Colloid Jin Yingguang quantitative analysis integrated machine and control method thereof - Google Patents
Colloid Jin Yingguang quantitative analysis integrated machine and control method thereof Download PDFInfo
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- CN106855515B CN106855515B CN201710046763.3A CN201710046763A CN106855515B CN 106855515 B CN106855515 B CN 106855515B CN 201710046763 A CN201710046763 A CN 201710046763A CN 106855515 B CN106855515 B CN 106855515B
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- 238000000034 method Methods 0.000 title claims abstract description 37
- 238000004445 quantitative analysis Methods 0.000 title claims abstract description 20
- 239000000084 colloidal system Substances 0.000 title claims abstract description 19
- 238000005286 illumination Methods 0.000 claims abstract description 25
- 238000010166 immunofluorescence Methods 0.000 claims abstract description 22
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 claims abstract description 21
- 238000001514 detection method Methods 0.000 claims description 70
- 238000003908 quality control method Methods 0.000 claims description 32
- 238000002347 injection Methods 0.000 claims description 17
- 239000007924 injection Substances 0.000 claims description 17
- 238000004458 analytical method Methods 0.000 claims description 10
- 230000003287 optical effect Effects 0.000 claims description 3
- 230000009286 beneficial effect Effects 0.000 description 8
- 238000010586 diagram Methods 0.000 description 2
- 238000007599 discharging Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000005070 sampling Methods 0.000 description 2
- 239000003086 colorant Substances 0.000 description 1
- 230000001678 irradiating effect Effects 0.000 description 1
- 230000000670 limiting effect Effects 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5302—Apparatus specially adapted for immunological test procedures
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- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
The invention discloses a colloid Jin Yingguang quantitative analysis integrated machine and a control method thereof, wherein the integrated machine can be used as a colloidal gold card reader or an immunofluorescence analyzer by the action of a microprocessor, an illumination unit, a shooting unit, an image processing unit, a laser and a first photoelectric sensor which are connected with the microprocessor, so that the cost is saved. The invention also provides a control method of the integrated machine, so that an operator does not need to distinguish the test paper card from the colloidal gold test paper card or the immunofluorescence test paper card in the use process, the experimental steps are reduced, and the experimental efficiency is improved.
Description
Technical Field
The invention relates to the technical field of clinical detection, in particular to a colloid Jin Yingguang quantitative analysis all-in-one machine and a control method thereof.
Background
The traditional clinical detection laboratory usually adopts a colloidal gold method or an immunofluorescence method to detect various physicochemical data, and common instruments include a colloidal gold reader, an immunofluorescence analyzer and the like. However, due to the lack of an instrument for integrating two instruments, a laboratory is usually equipped with the two instruments, and the different test cards aimed at by the two instruments need to be marked and distinguished by a laboratory staff, which is not beneficial to improving the experimental efficiency.
Disclosure of Invention
The invention aims to solve the problems that the existing clinical detection instrument is single and can not detect a plurality of physicochemical data by adopting a colloidal gold method or an immunofluorescence method, and provides a colloid Jin Yingguang quantitative analysis integrated machine which has the characteristics of detecting a plurality of physicochemical data by adopting the colloidal gold method and the immunofluorescence method.
In order to achieve the purpose, the colloid Jin Yingguang quantitative analysis all-in-one machine designed by the invention comprises a machine body, a sample inlet and outlet opening and a display screen, wherein the sample inlet and outlet opening and the display screen are arranged on the machine body; a microprocessor, an illumination unit connected with the microprocessor, a shooting unit, an image processing unit, a laser and a first photoelectric sensor are arranged in the machine body;
the sample inlet and outlet opening is used for placing or taking out the test paper card;
the illumination unit is used for outputting illumination light to the test paper card;
the shooting unit is used for carrying out optical shooting on the test paper card and obtaining a shooting result;
the image processing unit is used for analyzing and processing the shooting result of the shooting unit to obtain a quality control line gray value and a detection line gray value of the test paper card;
the laser is used for carrying out laser irradiation on the test paper card so that the test paper card emits fluorescence;
the first photoelectric sensor is used for receiving the fluorescence and processing the fluorescence into an electric signal according to the fluorescence intensity to be sent to the microprocessor;
the microprocessor obtains a detection result by calculating the ratio of the gray value of the quality control line to the gray value of the detection line or processing the electric signal of the first photoelectric sensor;
the display screen is used for displaying the detection result.
Further, a sample injection device which is connected with the microprocessor and extends out of the machine body through the sample inlet and outlet opening is also arranged in the machine body; the sample injection device comprises a horizontal test paper tray for containing a plurality of test paper cards and a horizontal sample injection tray for fixing the test paper tray; the upper end face of the test paper tray is provided with a plurality of clamping grooves which are correspondingly matched with the test paper cards; the bottom of the sample feeding disc is provided with a sliding rail, and the side wall of the sample feeding disc is provided with a rack arranged along the direction of the side wall; a gear matched with the rack and a motor for driving the gear to rotate are also arranged in the machine body; the motor is connected with the microprocessor; the lighting unit, the shooting unit, the laser and the first photoelectric sensor are arranged right above the test paper tray.
The beneficial effects of the above-mentioned further scheme are: the plurality of test paper cards are placed in the clamping grooves of the test paper disc, so that one test paper disc can be fixedly provided with the plurality of test paper cards, and the detection efficiency is improved; and through the action of the sample feeding disc, the rack, the gear and the motor on the side wall of the sample feeding disc, sample feeding and sample discharging can be automatically controlled, sample feeding is stable, and the problems of inclination and shaking of the test paper card caused by manual operation can be avoided.
Preferably, the machine body is also internally provided with second photoelectric sensors arranged at two sides of the sample injection device, one side of each clamping groove of the test paper disc is provided with a convex baffle for cutting off signals of the second photoelectric sensors, and the second photoelectric sensors are connected with the microprocessor.
The beneficial effects of the above-mentioned preferred scheme are: through the positioning effect of the second photoelectric sensor and the baffle, the microprocessor can effectively control the motor to work, so that the moving distance of the sample feeding disc is limited to be the distance between two test paper cards each time when the test paper cards are detected, and each time one test paper card is detected, the sample feeding disc moves forward once to enable the next test paper card to be in a region to be detected.
Further, the test paper card also comprises an identification code which can be attached to the test paper tray or the test paper card and is used for distinguishing the test paper card into a colloidal gold test paper card or an immunofluorescence test paper card.
The beneficial effects of the above-mentioned further scheme are: through the effect of identification code, can make the all-in-one automatic divide into colloidal gold test paper card or immunofluorescence test paper card with the test paper card to carry out corresponding detection to this test paper card, less unnecessary experimental step improves detection efficiency.
Preferably, the test paper disc is detachably connected with the sample feeding disc.
The beneficial effects of the above-mentioned preferred scheme are: through with test paper dish and sampling dish detachable connection for after the test paper dish was dismantled, place test paper card more convenient and fast.
Further, a positioning through hole is formed in the paper test disc; and the sample feeding disc is provided with a positioning pin matched with the positioning through hole.
The beneficial effects of the above-mentioned further scheme are: through the action of the locating pin and the locating through hole, the sample tray is prevented from being displaced with the sample tray in the moving process.
Preferably, the test paper card is a colloidal gold test paper card or an immunofluorescence test paper card.
The beneficial effects of the invention are as follows: through the functions of the microprocessor, the lighting unit, the shooting unit, the image processing unit, the laser and the first photoelectric sensor which are connected with the microprocessor, the integrated machine can be used as a colloidal gold card reader and an immunofluorescence analyzer, the cost is saved, a plurality of test paper cards coated with samples can be detected by only one sample injection, the test paper cards are not required to be distinguished to be colloidal gold test paper cards or immunofluorescence test paper cards, the experimental steps are reduced, and the experimental efficiency is improved.
The invention also provides a control method of the colloid Jin Yingguang quantitative analysis all-in-one machine, which comprises the following steps:
step 1) putting the test paper card into a machine body through a sample inlet and outlet opening;
step 2) the test paper card enters an operation area of the illumination unit, the microprocessor controls the illumination unit to output illumination light to the test paper card, and controls the shooting unit to optically shoot the test paper card to obtain a shooting result;
step 3) the microprocessor controls the image processing unit to analyze and process the shooting result of the step 2),
when the analysis processing result obtains the gray value of the test paper card detection line and the gray value of the quality control line, obtaining a detection result according to the gray value of the quality control line and the gray value of the detection line or calculating the ratio of the gray value and the gray value of the quality control line, and performing step 7);
when the analysis processing result shows that the quality control line gray value and the detection line gray value are not detected on the test paper card, performing the step 4);
step 4) the microprocessor controls the laser to irradiate the test paper card so that the test paper card emits fluorescence;
step 5), the first photoelectric sensor receives the fluorescence in the step 4) and processes the fluorescence into an electric signal to be sent to the microprocessor;
step 6) the microprocessor processes the electric signals in the step 5) to obtain a fluorescent strip result;
when the fluorescent strip result is obtained, obtaining a quality control line fluorescent intensity value and a detection line fluorescent intensity value of the test paper card, and obtaining a detection result according to the quality control line fluorescent intensity value, the detection line fluorescent intensity value or the ratio of the two values;
step 7), the microprocessor feeds back the detection result to the display screen for display;
step 8) returning to the step 2) for the next test paper card detection.
The beneficial effects of the invention are as follows: the method detects the sample without distinguishing the colloidal gold test paper card or the immunofluorescence test paper card, and the detection results are displayed through the display screen, so that the experimental efficiency is improved.
Drawings
Fig. 1 is a schematic perspective view of a quantitative analysis integrated machine for colloid Jin Yingguang in the present invention.
Fig. 2 is a schematic diagram of a quantitative analysis integrated machine for colloid Jin Yingguang in the present invention.
Fig. 3 is a schematic structural diagram of a test paper disc of the colloid Jin Yingguang quantitative analysis integrated machine of the present invention mounted on a sample feeding disc.
Fig. 4 is a schematic perspective view of the sample tray in fig. 3.
Fig. 5 is a schematic top view of the test paper tray of fig. 3.
Fig. 6 is a schematic perspective view of the test paper tray of fig. 5 with the test paper card removed.
FIG. 7 is a schematic cross-sectional view of the A-A plane of FIG. 6.
In the figure, 1. A machine body; 2. a sample inlet and outlet opening; 3. a display screen; 4. a microprocessor; 5. a lighting unit; 6. a photographing unit; 7. an image processing unit; 8. a laser; 9. a first photosensor; 10. test paper card; 11. a test paper tray; 12. a sample introduction disc; 13. a clamping groove; 14. a rack; 15. a gear; 16. a motor; 17. a second photosensor; 18. a baffle; 19. numbering; 20. positioning the through hole; 21. a positioning pin; 22. and a sample injection device.
Detailed Description
The invention is described in further detail below with reference to the drawings and specific examples.
The colloid Jin Yingguang quantitative analysis all-in-one machine shown in fig. 1-7 comprises a machine body 1, a sample inlet and outlet opening 2 and a display screen 3, wherein the sample inlet and outlet opening 2 and the display screen 3 are arranged on the machine body 1; the machine body 1 is internally provided with a microprocessor 4, an illumination unit 5 connected with the microprocessor 4, a shooting unit 6, an image processing unit 7, a laser 8 and a first photoelectric sensor 9;
the sample inlet and outlet opening 2 is used for putting in or taking out the test paper card 10;
the illumination unit 5 is used for outputting illumination light to the test paper card 10;
the shooting unit 6 is used for carrying out optical shooting on the test paper card 10 and obtaining a shooting result;
the image processing unit 7 is used for analyzing and processing the shooting result of the shooting unit 6 to obtain a quality control line gray value and a detection line gray value of the test paper card 10;
the laser 8 is used for irradiating laser to the test paper card 10 to make the test paper card 10 emit fluorescence;
the first photoelectric sensor 9 is used for receiving fluorescence and processing the fluorescence into an electric signal according to the fluorescence intensity to be sent to the microprocessor 4;
the microprocessor 4 obtains a detection result by calculating the ratio of the gray value of the quality control line to the gray value of the detection line or processing the electric signal of the first photoelectric sensor 9;
the display screen 3 is used for displaying the detection result.
The body 1 is also internally provided with a sample injection device 22 which is connected with the microprocessor 4 and extends out of the body 1 through the sample inlet and outlet opening 2; the sample introduction device 22 includes a horizontal sample introduction tray 11 for holding a plurality of sample cards 10 and a horizontal sample introduction tray 12 for fixing the sample introduction tray 11; the upper end surface of the test paper tray 11 is provided with a plurality of clamping grooves 13 correspondingly matched with the test paper cards 10; a sliding rail (not shown) is arranged at the bottom of the sample introduction disc 12, and racks 14 arranged along the side wall direction are arranged on the side wall of the sample introduction disc 12; a gear 15 matched with the rack 14 and a motor 16 for driving the gear 15 to rotate are also arranged in the machine body 1; the motor 16 is connected with the microprocessor 4; the illumination unit 5, the photographing unit 6, the laser 8, and the first photosensor 9 are disposed directly above the test paper tray 11. By placing a plurality of test paper cards 10 in the clamping groove 13 of the test paper disc 11, one test paper disc 11 can fixedly arrange the plurality of test paper cards 10, so that the detection efficiency is improved; and through the action of the sample feeding disc 12, the rack 14, the gear 15 and the motor 16 on the side wall of the sample feeding disc 12, sample feeding and sample discharging can be automatically controlled, sample feeding is stable, and the problems of inclination, shaking and the like of the test paper card 10 caused by manual operation can be avoided.
The machine body 1 is also internally provided with second photoelectric sensors 17 arranged at two sides of the sample introduction device 22, the paper test disc 11 is provided with a convex baffle 18 for cutting off signals of the second photoelectric sensors 17 at one side of each clamping groove 13, and the second photoelectric sensors 17 are connected with the microprocessor 4. Through the positioning action of the second photoelectric sensor 17 and the baffle plate 18, the microprocessor 4 can effectively control the motor 16 to work, so that the moving distance of the sample introduction disc 12 each time is limited to be the distance between two test paper cards 10 when the test paper cards 10 are detected, and each time one test paper card 10 is detected, the sample introduction disc 12 moves forward once to enable the next test paper card 10 to be in the area to be detected.
And an identification code (not shown) which can be attached to the test paper tray 11 or the test paper card 10 and is used for distinguishing the test paper card 10 into a colloidal gold test paper card or an immunofluorescence test paper card. The identification code can be a two-dimensional code or a paper sheet coated with different colors; the experimenter pastes the identification code representing the colloidal gold test paper card at the corresponding position of the corresponding colloidal gold test paper card, and pastes the identification code representing the immunofluorescence test paper card at the corresponding position of the corresponding immunofluorescence test paper card. In the process of shooting the test paper card 10, the shooting unit 6 shoots the identification code and uploads the identification code to the image processing unit 7 for processing to obtain information corresponding to the identification code, and the microprocessor 4 judges that the test paper card 10 is a colloidal gold test paper card or an immunofluorescence test paper card according to the information, so that corresponding operation is performed.
The upper end surface of the test tray 11 is provided with a number 19 or a label for identifying the card slot 13 on the side of each card slot 13. By arranging the serial number 19 or the label corresponding to the clamping groove 13 on the test paper disc 11, the data of the corresponding test paper card 10 can be conveniently recorded, and the data disorder is prevented.
The sample tray 11 is detachably connected with the sample tray 12. Through with test paper tray 11 and sampling dish 12 detachable connection for after test paper tray 11 dismantles, place test paper card 10 more convenient and fast.
The test paper tray 11 is provided with a positioning through hole 20; the sample introduction disc 12 is provided with a positioning pin 21 matched with the positioning through hole 20. The sample tray 12 is prevented from being displaced from the test tray 11 during the movement by the action of the positioning pins 21 and the positioning through holes 20.
The test paper card 10 is a colloidal gold test paper card or an immunofluorescence test paper card.
Through the functions of the microprocessor 4, the illumination unit 5, the shooting unit 6, the image processing unit 7, the laser 8 and the first photoelectric sensor 9 which are connected with the microprocessor, the integrated machine can be used as a colloidal gold card reader and an immunofluorescence analyzer, the cost is saved, a plurality of test paper cards 10 coated with samples can be detected only by one sample injection, and the test paper cards 10 are not required to be distinguished to be colloidal gold test paper cards or immunofluorescence test paper cards, so that experimental steps are reduced, and experimental efficiency is improved.
The invention also provides a control method of the colloid Jin Yingguang quantitative analysis all-in-one machine, which comprises the following steps:
step 1), putting a test paper card 10 into a machine body 1 through a sample inlet and outlet opening 2;
step 2), the test paper card 10 enters an operation area of the illumination unit 5, the microprocessor 4 controls the illumination unit 5 to output illumination light to the test paper card 10, and controls the shooting unit 6 to optically shoot the test paper card 10 to obtain a shooting result;
step 3) the microprocessor 4 controls the image processing unit 7 to analyze and process the shooting result of the step 2),
when the analysis processing result is obtained to obtain the gray value of the detection line and the gray value of the quality control line of the test paper card 10, calculating the ratio of the gray value of the quality control line to the gray value of the detection line to obtain a detection result, and performing the step 7);
when the analysis processing result shows that the gray value of the quality control line of the test paper card 10 is obtained and the gray value of the detection line is not obtained, the detection result is 0, and the step 7 is carried out;
when the analysis processing result shows that the gray value of the detection line of the test paper card 10 is obtained and the gray value of the quality control line is not obtained, the detection result is invalid, and the step 7 is carried out;
when the analysis processing result shows that the quality control line gray value and the detection line gray value are not detected on the test paper card 10, performing step 4);
step 4), the microprocessor 4 controls the laser 8 to irradiate the test paper card 10 with laser, so that the test paper card 10 emits fluorescence;
step 5) the first photoelectric sensor 9 receives the fluorescence in the step 4) and processes the fluorescence into an electric signal to be sent to the microprocessor 4;
step 6) the microprocessor 4 processes the electric signal in the step 5) to obtain a fluorescent strip result;
when the fluorescent strip result is obtained to obtain a quality control line fluorescent intensity value and a detection line fluorescent intensity value of the test paper card 10, calculating the ratio of the quality control line fluorescent intensity value to the detection line fluorescent intensity value to obtain a detection result;
when the fluorescent strip result shows that the fluorescent intensity value of the quality control line of the test paper card 10 is obtained and the fluorescent intensity value of the detection line is not obtained, the detection result is 0;
when the fluorescent strip result is obtained, the fluorescent intensity value of the test paper card 10 detection line is obtained, and the quality control line fluorescent intensity value is not obtained, the detection result is invalid;
step 7), the microprocessor 4 feeds back the detection result to the display screen 3 for display;
step 8) returning to step 2) for the next test card 10 detection.
It should be noted that the body 1 of the present invention is provided with corresponding control switch buttons.
When in use, the test paper cards 10 coated with the samples are arranged in the clamping grooves 13 of the test paper disc 11, and the test paper cards 10 and the corresponding numbers 19 are marked, so that the test results can be recorded conveniently; after the test paper card 10 is placed on the test paper tray 11, the test paper tray 11 is fixed on the sample feeding tray 12, and the positioning pin 21 is matched with the positioning through hole 20. When sample injection is needed, the microprocessor 4 controls the motor 16 to work through a corresponding switch button on the machine body 1 so as to drive the gear 15 to rotate, the rack 14 matched with the motor is the sample injection disc 12 for sample injection, and the sample injection disc 12 advances to a corresponding position due to the action of the second photoelectric sensor 17 and the baffle piece 18; the microprocessor 4 controls the illumination unit 5 to output illumination light to the first test paper card 10 on the test paper disc 11, and controls the shooting unit 6 to optically shoot the test paper card 10 to obtain a shooting result; the microprocessor 4 controls the image processing unit 7 to analyze and process the shooting result to obtain the gray value of the quality control line and the gray value of the detection line of the test paper card 10; the microprocessor 4 obtains a detection result by calculating the ratio of the gray value of the quality control line to the gray value of the detection line, and feeds back the detection result to the display screen 3 for display, then the microprocessor 4 controls the motor 16 to work, and the sample tray 12 moves forward by the distance between the two test paper cards 10 under the limiting action of the second photoelectric sensor 17 and the baffle sheet 18, so that the second test paper card 10 on the paper tray is in the illumination range of the illumination unit 5, and the operation is sequentially performed.
If the microprocessor 4 cannot obtain the detection result of the first test paper card 10 by calculating the ratio of the gray value of the quality control line to the gray value of the detection line, it can be inferred that the first test paper card 10 is an invalid card or an immunofluorescence test paper card, and the microprocessor 4 controls the laser 8 to irradiate the laser to the test paper card 10, so that the test paper card 10 emits fluorescence; the first photoelectric sensor 9 receives the fluorescence and processes the fluorescence into an electric signal to be sent to the microprocessor 4; the microprocessor 4 processes the electric signal to obtain a detection result, and feeds back the detection result to the display screen 3 for display, so that the next test paper card 10 is detected; and sequentially carrying out the steps until the test paper cards 10 on the test paper tray 11 are completely detected, controlling the motor 16 to work by the microprocessor 4, and withdrawing the sample feeding tray 12 from the sample inlet and outlet opening 2.
Claims (7)
1. A control method of a colloid Jin Yingguang quantitative analysis integrated machine is characterized by comprising the following steps of: the colloid Jin Yingguang quantitative analysis all-in-one machine comprises a machine body (1), a sample inlet and outlet opening (2) and a display screen (3), wherein the sample inlet and outlet opening (2) and the display screen (3) are formed in the machine body (1); a microprocessor (4), an illumination unit (5) connected with the microprocessor (4), a shooting unit (6), an image processing unit (7), a laser (8) and a first photoelectric sensor (9) are arranged in the machine body (1); the sample inlet and outlet opening (2) is used for placing or taking out the test paper card (10); the illumination unit (5) is used for outputting illumination light to the test paper card (10); the shooting unit (6) is used for carrying out optical shooting on the test paper card (10) and obtaining a shooting result; the image processing unit (7) is used for analyzing and processing the shooting result of the shooting unit (6) to obtain a quality control line gray value and a detection line gray value of the test paper card (10); the laser (8) is used for carrying out laser irradiation on the test paper card (10) so that the test paper card (10) emits fluorescence; the first photoelectric sensor (9) is used for receiving the fluorescence and processing the fluorescence into an electric signal according to the fluorescence intensity to be sent to the microprocessor (4); the microprocessor (4) obtains a detection result by calculating the ratio of the gray value of the quality control line to the gray value of the detection line or processing the electric signal of the first photoelectric sensor (9); the display screen (3) is used for displaying the detection result;
the control method comprises the following steps:
step 1), putting a test paper card (10) into a machine body (1) through a sample inlet and outlet opening (2);
step 2), a test paper card (10) enters an operation area of an illumination unit (5), a microprocessor (4) controls the illumination unit (5) to output illumination light to the test paper card (10), and controls a shooting unit (6) to optically shoot the test paper card (10) to obtain a shooting result;
step 3) the microprocessor (4) controls the image processing unit (7) to analyze and process the shooting result of the step 2):
when the analysis processing result is used for obtaining the gray value of the test paper card detection line and the gray value of the quality control line, calculating the ratio of the gray value of the quality control line to the gray value of the detection line to obtain a detection result, and performing the step 7);
when the analysis processing result obtains the gray value of the quality control line of the test paper card and the gray value of the detection line is not obtained, the detection result is 0, and the step 7) is carried out;
when the analysis processing result obtains the gray value of the test paper card detection line and the gray value of the quality control line is not obtained, the detection result is invalid, and the step 7) is carried out;
when the analysis processing result shows that the quality control line gray value and the detection line gray value are not detected on the test paper card (10), performing the step 4);
step 4), the microprocessor (4) controls the laser (8) to irradiate the test paper card (10) with laser so that the test paper card (10) emits fluorescence;
step 5) a first photoelectric sensor (9) receives the fluorescence in the step 4) and processes the fluorescence into an electric signal to be sent to the microprocessor (4);
step 6) the microprocessor (4) processes the electrical signal in step 5) to obtain a fluorescence band result:
when the fluorescent strip result is obtained, calculating the ratio of the quality control line fluorescent intensity value to the detection line fluorescent intensity value to obtain a detection result;
when the fluorescent strip result is obtained, the quality control line fluorescent intensity value of the test paper card is obtained, and the detection line fluorescent intensity value is not obtained, the detection result is 0;
when the fluorescent strip results obtain the fluorescent intensity value of the test paper card detection line and the fluorescent intensity value of the quality control line is not obtained, the detection result is invalid;
step 7), the microprocessor (4) feeds back the detection result to the display screen (3) for display;
step 8) returning to the step 2) for the next test paper card (10) detection.
2. The method for controlling a gel Jin Yingguang quantitative analysis all-in-one machine according to claim 1, wherein: a sample injection device (22) which is connected with the microprocessor (4) and extends out of the machine body (1) through the sample inlet and outlet opening (2) is also arranged in the machine body (1); the sample injection device (22) comprises a horizontal test paper disc (11) for containing a plurality of test paper cards (10) and a horizontal sample injection disc (12) for fixing the test paper disc (11); the upper end face of the test paper disc (11) is provided with a plurality of clamping grooves (13) which are correspondingly matched with the test paper cards (10); the bottom of the sample feeding disc (12) is provided with a sliding rail, and the side wall of the sample feeding disc (12) is provided with a rack (14) arranged along the side wall direction; a gear (15) matched with the rack (14) and a motor (16) for driving the gear (15) to rotate are also arranged in the machine body (1); the motor (16) is connected with the microprocessor (4); the illuminating unit (5), the shooting unit (6), the laser (8) and the first photoelectric sensor (9) are arranged right above the test paper disc (11).
3. The control method of the integrated quantitative analysis machine for colloid Jin Yingguang according to claim 2, wherein: the device is characterized in that second photoelectric sensors (17) arranged on two sides of the sample injection device (22) are further arranged in the machine body (1), a convex baffle (18) for cutting off signals of the second photoelectric sensors (17) is arranged on one side of each clamping groove (13) of the test paper disc (11), and the second photoelectric sensors (17) are connected with the microprocessor (4).
4. A control method of a colloid Jin Yingguang quantitative analysis all-in-one machine according to claim 2 or 3, wherein: the test paper card also comprises an identification code which can be adhered to the test paper disc (11) or the test paper card (10) and is used for distinguishing the test paper card (10) into a colloidal gold test paper card or an immunofluorescence test paper card.
5. A control method of a colloid Jin Yingguang quantitative analysis all-in-one machine according to claim 2 or 3, wherein: the test paper disc (11) is detachably connected with the sample injection disc (12).
6. A control method of a colloid Jin Yingguang quantitative analysis all-in-one machine according to claim 2 or 3, wherein: a positioning through hole (20) is formed in the test paper disc (11); and a positioning pin (21) matched with the positioning through hole (20) is arranged on the sample feeding disc (12).
7. The control method of the integrated quantitative analysis machine for colloid Jin Yingguang according to claim 1, 2 or 3, wherein: the test paper card (10) is a colloidal gold test paper card or an immunofluorescence test paper card.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710046763.3A CN106855515B (en) | 2017-01-22 | 2017-01-22 | Colloid Jin Yingguang quantitative analysis integrated machine and control method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710046763.3A CN106855515B (en) | 2017-01-22 | 2017-01-22 | Colloid Jin Yingguang quantitative analysis integrated machine and control method thereof |
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| CN108303423A (en) * | 2018-02-12 | 2018-07-20 | 广州眺望电子科技有限公司 | A kind of simple efficiently fluorescin reagent card digital independent device and method |
| CN109738622B (en) * | 2018-12-29 | 2020-12-11 | 北京化工大学 | Lateral flow paper strip rapid detection device based on micro-fluidic chip |
| CN110531097A (en) * | 2019-10-11 | 2019-12-03 | 北京中航赛维生物科技有限公司 | A kind of colloidal gold fluorescence one immunity analysis instrument |
| CN111795959A (en) * | 2020-07-24 | 2020-10-20 | 芯动森林(重庆)医疗科技有限公司 | Colloidal gold and fluorescence two-in-one data acquisition device and method |
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