CN106829202B - Constant low temperature storage system and the method for removing Dewar fog - Google Patents
Constant low temperature storage system and the method for removing Dewar fog Download PDFInfo
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- CN106829202B CN106829202B CN201710024975.1A CN201710024975A CN106829202B CN 106829202 B CN106829202 B CN 106829202B CN 201710024975 A CN201710024975 A CN 201710024975A CN 106829202 B CN106829202 B CN 106829202B
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D81/00—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents
- B65D81/18—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents providing specific environment for contents, e.g. temperature above or below ambient
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D81/00—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents
- B65D81/24—Adaptations for preventing deterioration or decay of contents; Applications to the container or packaging material of food preservatives, fungicides, pesticides or animal repellants
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D85/00—Containers, packaging elements or packages, specially adapted for particular articles or materials
- B65D85/50—Containers, packaging elements or packages, specially adapted for particular articles or materials for living organisms, articles or materials sensitive to changes of environment or atmospheric conditions, e.g. land animals, birds, fish, water plants, non-aquatic plants, flower bulbs, cut flowers or foliage
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Abstract
A kind of constant low temperature storage system and removal Dewar fog method, which includes Dewar: inside having sample storage room and refrigerant room, at least one that refrigerant is imported cold air in refrigerant room by being poured into the refrigerant input channel of refrigerant room, the closed barrier between sample storage room and refrigerant room, at least one outside Dewar for the airway tube external of refrigerant room gas discharge Dewar and at least one to the internal ventilation conduit of sample storage room；Remove Dewar fog method are as follows: dry filter air is pressed into refrigerant room by airway tube external and/or refrigerant input channel, sample storage room demisting is flowed by internal ventilation conduit after so that the gas is blistered in refrigerant and/or accelerates sample freezing.A large amount of cold air enter sample storage room when the present invention can avoid supplement refrigerant, guarantee that storage temperature is constant, improve refrigerant service efficiency, can drive away sample storage room fog by being pressed into dry filter air to the system and/or are rapidly frozen sample.
Description
Technical field
The present invention relates to low temperature technical field of memory more particularly to a kind of constant low temperature storages for storing biological sample
System and the method for removing Dewar fog.
Background technique
Vacuum dewar (vacuum flask) equipped with liquid nitrogen (refrigerant) is widely used in the cryo-conservation of biological sample.Sample
Cryo-conservation can both be realized by being stored in liquid nitrogen liquid, can also be by real in the cold air that is stored on liquid nitrogen liquid level
It is existing.Sample is stored in cold air in Dewar, can be described as gas phase storage, the existing advantage of this kind of storage mode also has disadvantage.Advantage
It is the propagation for occurring to pollute mutually and prevent infective agents between each sample that can avoid being co-located in a liquid nitrogen bath.Its disadvantage
It is not have since the cold air temperature on liquid nitrogen liquid level changes greatly in the volatilization intracorporal steady temperature of state liquid nitrogen liquid.
The rising of cold air temperature in Dewar and the height of liquid nitrogen liquid are proportional.To reduce the chill temperature on liquid nitrogen liquid level
Gradient is spent, sample is stored in the gas phase memory space of temperature more stable homogeneous, is in the inner wall of Dewar in the prior art
Add a temperature conduction layer.To make sample storage in the gas phase memory space of more steady temperature, can also be passed with temperature
There are one or more temperature cabinets of heat-insulated container (to refer to United States Patent (USP) for setting in the gas-phase space of the property led inner wall
US7278278B2).
Cold air temperature in Dewar can be disturbed when supplementing liquid nitrogen.When filling liquid nitrogen, liquid nitrogen can be from source pressure
Place loses pressure and boils with hotlist face contact.Liquid nitrogen can also be sent out when reaching Dewar after flowing through pipeline and hose from transfer cask
Raw boiling.It is cold as the temperature of gas temperature meeting and liquid nitrogen itself that liquid nitrogen mixes.The long pipeline of connection center transfer cask
It can be due to the function reduction there are bulk gas and liquid nitrogen mixing.In prior art, as shown in Figure 1, cryogenic storage system
Including Dewar 1 ', Dewar 1 ' is Dewar bottle body 2 ' and covers the 3 ' group of Dewar lid made of the foamed material on Dewar bottle body 2 '
At it is true between Dewar outer wall 4 ' and Dewar inner wall 5 ' that Dewar bottle body 2 ' is connected and composed by Dewar outer wall 4 ' and Dewar inner wall 5 '
Absolutely empty 6 ', the inner cavity lower part of Dewar bottle body 2 ' is refrigerant pond 7 ', is inside contained with refrigerant liquid nitrogen.Dewar bottle body 2 ' it is interior
Space of the chamber between refrigerant pond 7 ' and Dewar lid 3 ' is sample storage room 8 ', and storage sample deposits in sample storage room
8 ', which also includes several layers of reflexive insulating layer (not shown) to completely cut off the external environment of Dewar 1 ' and the sample of Dewar 1 '
Product locker room 8 '.Dewar lid 3 ' provides thermodynamics isolation at the top of Dewar 1 ', and provides into the logical of sample storage room 8 '
Road can be packed into and take out storage sample.One refrigerant input channel 9 ' is for being periodically perfused liquid refrigerant to refrigerant
In pond 7 ', the duration of refrigerant evaporates the low temperature to maintain the inside of Dewar 1 ', and liquid coolant is maintained at sufficiently low level
It is upper to can guarantee sufficient sample storage room 8 ' just to place storage sample.
When liquid nitrogen and its gaseous mixture are when being filled into the inner cavity of Dewar bottle body 2 ', cold air can only pass through Dewar lid 3 '
It is escaped with the gap 10 ' on 2 ' top of Dewar bottle body.In this process, cold air must rise through 2 ' inner cavity top of Dewar bottle body
Sample storage room 8 ', this will lead to addition liquid nitrogen when gas phase in temperature decline.
In order to reduce the gas flow mixed with liquid nitrogen, some streamer systems can use " hot-gas bypass " system in filling.
Hot gas by-pass system can be before liquid nitrogen perfusion, and the hot gas in refrigerant input channel 9 ', which is ejected, prevents it from entering Dewar
1 ' inner space.It can maintain stable 1 ' internal temperature gradient of Dewar and prevent liquid nitrogen excessive evaporation to improve using effect
Rate.This functionality is useful especially for longer refrigerant input channel 9 '.One hot gas by-pass system generally comprises bypass electricity
Magnet valve door, temperature sensor, silencing means, refrigerant input channel 9 ' and electronic controller.When starting is perfused, electromagnetism is bypassed
Valve is first turned on, and hot gas is made to be discharged to ambient enviroment by silencing means；When temperature sensor detection temperature be reduced to it is pre-
When the temperature first designed, bypass solenoid valve door, which is closed, terminates hot-gas bypass function.General this temperature setting is -70 degree, but its
It can be adjusted.
When such system starts perfusion, the gas and liquid nitrogen of flowing by from supply line and hose water conservancy diversion to Du
In watt 1 ' environment in addition, so that supply line and hose are first fully cooled, fluid can be fully cooled before entering Dewar 1 '.
The main purpose of these systems is that enter Dewar 1 ' interior for hot gas before liquid nitrogen is perfused in order to prevent.Once there is enough liquid nitrogen streams
When dynamic, the gas mixed with liquid nitrogen has with the temperature as liquid nitrogen, and hot-air bypath can stop working.Liquid nitrogen and mixed with it
The cold air of conjunction is all allowed to flow into Dewar 1 '.But in entire filling process, still has a certain number of cold air and enter
In Dewar 1 '.When cold air flows through sample storage room 8 ' and overflows Dewar 1 ', due to the effect of convection current and conduction, these cold air meetings
Disturb the temperature of the storage sample in Dewar 1 '.
Existing United States Patent (USP) 7,278,278B2 is by placing multiple temperature cabinets in Dewar bottle intracavity, by each storage sample
Product are individually positioned in each temperature cabinet, can reduce gas phase temperature disturbing phenomenon when Dewar perfusion in this way, while filling in Dewar
There is provided necessary electric heating mechanism also during note to maintain the constant of gas phase temperature.However, insulation can only reduce the change of temperature
Change amount can not prevent this variation phenomenon.Feedback control system confirmation is before heater compensates, in memory space still
So there is thermal perturbation to a certain extent.
Summary of the invention
The purpose of the present invention is to provide a kind of constant low temperature storage system and the methods for removing Dewar fog, are supplementing
Sample storage space is avoided passing through when refrigerant, avoids the temperature of disturbed sample memory space, improves the use to refrigerant
Efficiency, and the frozen air by being inversely pressed into dry filter into the system can wash away sample storage room, be driven with reaching
Except fog, it is rapidly frozen the purpose of sample.
To achieve the goals above, the present invention provides a kind of constant low temperature storage system, comprising:
Dewar: the sample storage room of sample container is equipped with for sample storage or placed in it and for accommodating liquid freezing
The refrigerant room of agent and cryogenic gases；
At least one refrigerant input channel: for by refrigerant by being filled into the refrigerant room outside Dewar；
Wherein further include:
Closed barrier: being set between the sample storage room and refrigerant room, prevents the two from carrying out gas exchanges；
At least one airway tube external: for the gas of the refrigerant room is direct without the sample storage room
It is discharged outside Dewar.
Preferably, further includes: at least one internal ventilation conduit: for importing the indoor part cold air of the refrigerant
The sample storage room.
Preferably, internal ventilation valve is provided on the internal ventilation conduit.
Preferably, the caliber of the internal ventilation conduit is less than the caliber of airway tube external.
Preferably, the internal ventilation conduit is connected to the airway tube external.
Preferably, the internal ventilation conduit is set between the inside and outside wall of Dewar or is set to the internal chamber wall of Dewar.
Preferably, the airway tube external is made of the first efferent duct and the second efferent duct, first efferent duct
One end is connected to the refrigerant room, and the other end of first efferent duct is connect by triple valve with second efferent duct one end,
The third end of the triple valve is connect with the internal ventilation tube at one end, the other end and the sample of the internal ventilation conduit
The connection of product locker room.
Preferably, the part that the airway tube external stretches out Dewar is provided with pressure relief valve.
Preferably, the part for Dewar being stretched out on the airway tube external is provided with exhaust branch pipe, on the exhaust branch pipe
It is provided with external aeration valve.
Preferably, it is provided at least one thermal insulation cabinet in the sample storage room, the sample storage is in the thermal insulation cabinet
Interior, the thermal insulation cabinet includes the cabinet body made of heat-transfer metal material, and the cabinet body includes at least one chamber, the cabinet body
Upper and lower surfaces be respectively arranged with multi-layer heat-transfer insulating layer.
Preferably, the inner chamber bottom surface of the Dewar is placed with the support platform made of heat conducting material, and the support is flat
The upper surface of platform is located at below the closed barrier.
Preferably, further include level gauging pipe for measuring cryodose, one end of the level gauging pipe is protruded into cold
Freeze agent, the other end of the level gauging pipe connects liquid level sensor after stretching out Dewar.
A method of removal Dewar fog, by dry filter air by outer in the constant low temperature storage system
Portion's airway and/or refrigerant input channel are pressed into the refrigerant room, make dry filter air in refrigerant room
It blisters in liquid coolant, demisting in sample storage room is flowed by internal ventilation conduit later and/or accelerates sample freezing.
After adopting the above scheme, constant low temperature storage system of the present invention has the advantages that
1, by the way that the closed barrier of air-tightness and at least one external aeration are arranged between sample storage room and refrigerant room
Conduit, when refrigerant is added, generated liquid nitrogen cold air is by airway tube external spilling Dewar in this way, without in Dewar
Absorbed inside heat ensure that the sample temperature stored when liquid nitrogen perfusion is unaffected；
2, by the way that at least one internal ventilation conduit is arranged between sample storage room and refrigerant room, refrigerant can be made
Indoor part cold air imports sample storage room, provides when supplement refrigerant gas from refrigerant room and passes through internal ventilation conduit
Sample storage room is reached, to realize the service efficiency improved to refrigerant；
3, by the way that internal ventilation valve is arranged on internal airway, it is designed the opening of internal ventilation conduit all
At adjustable, keep the gas-flow resistance of internal ventilation conduit and its outlet adjustable, so as to when carrying out refrigerant perfusion, convection current
The air-flow of Dewar generates optimal regulating effect out, interphase is perfused in refrigerant, airway tube external is closed, and internal ventilation is opened
Valve can make the gas of refrigerant room lead to sample storage room, improve the service efficiency to refrigerant；
4, by the caliber less than airway tube external for doing the caliber of internal ventilation conduit, in this way in past refrigerant room
When refrigerant is perfused, the cold air of refrigerant room can be preferentially discharged from the big airway tube external of caliber, and after being perfused, it closes
The cold air of airway tube external, such refrigerant room can be passed through sample storage room from internal ventilation conduit, by simply tying
Structure realizes the temperature that frozen gas disturbed sample locker room is on the one hand avoided when supplementing refrigerant, in addition also substantially increases
The service efficiency of refrigerant；
5, by the way that thermal insulation cabinet is arranged in sample storage room, by sample storage in thermal insulation cabinet, and it is following in closed barrier
Platform is set, design keeps the temperature of refrigerant room more uniform in this way, and the temperature independent of institute's liquid storage nitrogen is uneven,
The stabilization for further ensuring sample storage temperature, keeps constant；
6, by setting level gauging pipe and liquid level sensor, user is facilitated to observe the level gauging that liquid level sensor provides
Refrigerant is supplemented in refrigerant room immediately when cryodose reaches the condition required supplementation with by the cryodose of pipe detection, is protected
Demonstrate,prove the stability of sample storage temperature；
7, it is provided by the invention it is a kind of remove Dewar fog method, by by dry filter air from outside by outside
Ventilation pipe or refrigerant input channel are pressed into the refrigerant room of Dewar, and dry filter air is made to be flowed into refrigerant room
And entered in sample storage room after blistering in liquid coolant, it can provide and wash away sample storage with dry filter air
Room is rapidly frozen the purpose of sample to reach expeling fog.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of existing cryogenic storage system；
Fig. 2 is one schematic cross-sectional view of embodiment of constant low temperature storage system of the present invention；
Fig. 3 is two schematic cross-sectional view of embodiment of constant low temperature storage system of the present invention；
Fig. 4 is three schematic cross-sectional view of embodiment of constant low temperature storage system of the present invention；
Fig. 5 is the example IV schematic cross-sectional view of constant low temperature storage system of the present invention；
Fig. 6 is five schematic cross-sectional view of embodiment of constant low temperature storage system of the present invention；
Fig. 7 is six schematic cross-sectional view of embodiment of constant low temperature storage system of the present invention；
Fig. 8 is seven schematic cross-sectional view of embodiment of constant low temperature storage system of the present invention；
Fig. 9 is eight schematic cross-sectional view of embodiment of constant low temperature storage system of the present invention；
Figure 10 is the schematic view of the front view of the embodiment nine of constant low temperature storage system of the present invention；
Figure 11 is that the embodiment nine of constant low temperature storage system of the present invention removes the overlooking structure diagram of foam lid.
Specific embodiment
The present invention is illustrated below according to attached drawing illustrated embodiment.This time disclosed embodiment can consider in all sides
Face is to illustrate, without limitation.The scope of the present invention is not illustrated to be limited by following implementation, only by claims
Shown in range, and including having all deformations in the same meaning and scope of the claims with scope of the claims.
Liquid nitrogen is used in the present invention and is only used as an application example, but application of the invention is not limited to liquid nitrogen.Cause
This, storage system described in the invention is also applied for other refrigerants.
Cryogenic storage system and its course of work according to the present invention are specifically illustrated with reference to the accompanying drawings of the specification.
One schematic cross-sectional view of embodiment of constant low temperature storage system of the present invention as shown in Figure 2, comprising:
Dewar 1: including bottle body 2 and it is installed on the foam lid 3 of 2 upper end of bottle body, bottle body 2 is by inner wall 4 and the integrated structure of outer wall 5
At being vacuum space 6 between inner wall 4 and outer wall 5, the inner cavity of bottle body 2 is equipped with for sample storage or places the sample of sample container
Product locker room 7 and refrigerant room 8 for accommodating liquid coolant and cryogenic gases, refrigerant room 8 is in sample storage room 7
Lower section；
At least one refrigerant input channel 9: for refrigerant to be filled into refrigerant room 8 by the outside of Dewar 1, this reality
It is a plurality of for applying refrigerant input channel 9 in example；
The closed barrier 10 of air-tightness: it is set to 1 inner cavity of Dewar between sample storage room 7 and refrigerant room 8, prevents sample
Product locker room 7 and refrigerant room 8 carry out gas exchanges；
At least one airway tube external 11: for directly arranging the gas in refrigerant room 8 without sample storage room 7
The outside of Dewar 1 out, airway tube external 11 is a plurality of in this embodiment.
The present embodiment between sample storage room 7 and refrigerant room 8 by being arranged the closed barrier 10 and at least one of air-tightness
Airway tube external 11, in this way when refrigerant is perfused in refrigerant room 8, closed barrier 10 can prevent perfusion refrigerant
When the liquid nitrogen cold air that generates enter in sample storage room 7, these liquid nitrogen cold air can pass through airway tube external 11 and overflow Dewar 1
Outside, without the absorbed inside heat in Dewar 1, it ensure that the sample temperature stored when liquid nitrogen perfusion is unaffected.
Two schematic cross-sectional view of embodiment of constant low temperature storage system of the present invention as shown in Figure 3, comprising:
Dewar 1: including bottle body 2 and it is installed on the foam lid 3 of 2 upper end of bottle body, bottle body 2 is by inner wall 4 and the integrated structure of outer wall 5
At being vacuum space 6 between inner wall 4 and outer wall 5, the inner cavity of bottle body 2 is equipped with for sample storage or places the sample of sample container
Product locker room 7 and refrigerant room 8 for accommodating liquid coolant and cryogenic gases, refrigerant room 8 is in sample storage room 7
Lower section；
At least one refrigerant input channel 9: for refrigerant to be filled into refrigerant room 8 by the outside of Dewar 1, this reality
It is a plurality of for applying refrigerant input channel 9 in example；
The closed barrier 10 of air-tightness: it is set to 1 inner cavity of Dewar between sample storage room 7 and refrigerant room 8, prevents sample
Product locker room 7 and refrigerant room 8 carry out gas exchanges；
At least one airway tube external 11: for directly arranging the gas in refrigerant room 8 without sample storage room 7
The outside of Dewar 1 out, airway tube external 11 is a plurality of in this embodiment；
At least one internal ventilation conduit 12: for depositing the part liquid nitrogen cold air generated in refrigerant room 8 importing sample
Storage chamber 7, internal ventilation conduit 12 is a plurality of in this embodiment, true between the inside and outside wall that internal ventilation conduit 12 passes through Dewar 1
Absolutely empty 6.
In the embodiment, airway tube external 11 is made of the first efferent duct 13 and the second efferent duct 14, the first efferent duct
13 one end is connected to refrigerant room 8, and the other end of the first efferent duct 13 passes through one end of triple valve 15 and the second efferent duct 14
Connection, the third end of triple valve 15 are connect with one end of internal ventilation conduit 12, the other end and sample of internal ventilation conduit 12
Locker room 7 is connected to, which is located in vacuum space 6.
When supplementing perfusion refrigerant to refrigerant room 8, the liquid nitrogen cold air of generation can be passed through by the setting of triple valve 15
Airway tube external 11 is directed to the outside for flowing to Dewar 1, and perfusion will not affect that the temperature in sample storage room 7；Work as perfusion
When stopping, liquid nitrogen cold air is directly sent to sample storage room 7 from refrigerant room 8 by internal ventilation conduit 12 by triple valve 15,
Not only have the characteristics of not influencing stored sample temperature in liquid nitrogen perfusion, while can also reach for the more preferable of liquid nitrogen
Service efficiency.By triple valve 15, can allow from refrigerant room 8 and come liquid nitrogen cold air lead to sample storage room 7 or
It is to lead to outside Dewar 1 easily to be converted.
Three schematic cross-sectional view of embodiment of constant low temperature storage system of the present invention as shown in Figure 4, comprising:
Dewar 1: including bottle body 2 and it is installed on the foam lid 3 of 2 upper end of bottle body, bottle body 2 is by inner wall 4 and the integrated structure of outer wall 5
At being vacuum space 6 between inner wall 4 and outer wall 5, the inner cavity of bottle body 2 is equipped with for sample storage or places the sample of sample container
Product locker room 7 and refrigerant room 8 for accommodating liquid coolant and cryogenic gases, refrigerant room 8 is in sample storage room 7
Lower section；
At least one refrigerant input channel 9: for refrigerant to be filled into refrigerant room 8 by the outside of Dewar 1, this reality
It is a plurality of for applying the refrigerant input channel 9 in example；
The closed barrier 10 of air-tightness: it is set to 1 inner cavity of Dewar between sample storage room 7 and refrigerant room 8, prevents sample
Product locker room 7 and refrigerant room 8 carry out gas exchanges；
At least one airway tube external 11: for directly arranging the gas in refrigerant room 8 without sample storage room 7
The outside of Dewar 1 out, airway tube external 11 is a plurality of in this embodiment, in the embodiment, is stretched out on airway tube external 11
The part of Dewar 1 is equipped with pressure relief valve 17, and the part that Dewar 1 is stretched out on airway tube external 11 is additionally provided with exhaust branch pipe
18, exhaust branch pipe 18 is equipped with external aeration valve 19；
At least one internal ventilation conduit 12: for depositing the part liquid nitrogen cold air generated in refrigerant room 8 importing sample
Storage chamber 7, internal ventilation conduit 12 is a plurality of in this embodiment, true between the inside and outside wall that internal ventilation conduit 12 passes through Dewar 1
Absolutely empty 6.In the present embodiment, the part that sample storage room is protruded on internal ventilation conduit 12 is equipped with internal ventilation valve 16.
The present embodiment when supplementing perfusion liquid nitrogen to refrigerant room 8, close, and external aeration valve by internal ventilation valve 16
Door 19 can be opened, and the liquid nitrogen cold air in refrigerant room 8 is forced to leave Dewar 1 by exhaust branch pipe 18, without depositing by sample
Storage chamber 7；When stopping is perfused in liquid nitrogen, internal ventilation valve 16 will be opened, and external aeration valve 19 will close, and force freezing
The liquid nitrogen cold air of agent room 8 flows into sample storage room 7 by internal ventilation conduit 12；If the pressure in refrigerant room 8 is unexpectedly
It increases, pressure relief valve 17 will be opened, and walk high-pressure liquid nitrogen cold air by the excessive release of airway tube external 11, be to protect
System is protected from the harm of pipeline blockage and valve invalidation.The present embodiment in addition to have the advantages that above-described embodiment two it is all other than,
Internal ventilation valve 16, pressure relief valve 17 and external aeration valve 19 in its system are arranged at closed vacuum space
Except 6, it is more convenient construction and maintenance.
The example IV schematic cross-sectional view of constant low temperature storage system of the present invention as shown in Figure 5, comprising:
Dewar 1: including bottle body 2 and it is installed on the foam lid 3 of 2 upper end of bottle body, bottle body 2 is by inner wall 4 and the integrated structure of outer wall 5
At being vacuum space 6 between inner wall 4 and outer wall 5, the inner cavity of bottle body 2 is equipped with for sample storage or places the sample of sample container
Product locker room 7 and refrigerant room 8 for accommodating liquid coolant and cryogenic gases, refrigerant room 8 is in sample storage room 7
Lower section；
At least one refrigerant input channel 9: for refrigerant to be filled into refrigerant room 8 by the outside of Dewar 1, this reality
It is a plurality of for applying refrigerant input channel 9 in example；
The closed barrier 10 of air-tightness: it is set to 1 inner cavity of Dewar between sample storage room 7 and refrigerant room 8, prevents sample
Product locker room 7 and refrigerant room 8 carry out gas exchanges；
At least one airway tube external 11: for directly arranging the gas in refrigerant room 8 without sample storage room 7
The outside of Dewar 1 out, airway tube external 11 is a plurality of in this embodiment, and the part of Dewar 1 is stretched out on airway tube external 11
Equipped with pressure relief valve 17, the part that Dewar 1 is stretched out on airway tube external 11 is additionally provided with exhaust branch pipe 18, exhaust branch pipe 18
It is equipped with external aeration valve 19；
At least one internal ventilation conduit 12: for depositing the part liquid nitrogen cold air generated in refrigerant room 8 importing sample
Storage chamber 7, internal ventilation conduit 12 is a plurality of in this embodiment, true between the inside and outside wall that internal ventilation conduit 12 passes through Dewar 1
Absolutely empty 6.In the present embodiment, the caliber of internal ventilation conduit 12 is less than the caliber of airway tube external 11, makes by refrigerant room 8
And the cold air come, it is preferentially exported except Dewar 1 by airway tube external 11 when refrigerant is perfused in supplement, to guarantee sample
7 internal temperature of locker room it is constant.In the embodiment, internal ventilation conduit 12 and airway tube external 11 are in vacuum space 6
It is connected.
When supplementing perfusion liquid nitrogen to refrigerant room 8, external aeration valve 19 is opened, due to the pipe of internal ventilation conduit 12
Diameter is less than the caliber of airway tube external 11, therefore liquid nitrogen cold air can be more likely to flow to airway tube external 11 rather than internal
Airway 12, more liquid nitrogen cold air will be flowed to except Dewar 1 by airway tube external 12, rather than flow to sample storage
Room 7；Liquid nitrogen be perfused stop when, external aeration valve 19 is closed, liquid nitrogen cold air will be by internal ventilation conduit 12 from freezing
Agent room 8 is flowed into sample storage room 7, if the pressure in refrigerant room 8 unexpectedly increases, pressure relief valve 17 will be opened,
It walks high-pressure liquid nitrogen cold air by the excessive release of airway tube external 11, pipeline blockage is protected from protection system and valve loses
The harm of effect.The present embodiment except have the advantages that above-described embodiment two it is all in addition to, valve quantity is not only minimum but also is set
Except vacuum space 6, construction and maintenance are facilitated.
Five schematic cross-sectional view of embodiment of constant low temperature storage system of the present invention as shown in Figure 6, comprising:
Dewar 1: including bottle body 2 and it is installed on the foam lid 3 of 2 upper end of bottle body, bottle body 2 is by inner wall 4 and the integrated structure of outer wall 5
At being vacuum space 6 between inner wall 4 and outer wall 5, the inner cavity of bottle body 2 is equipped with for sample storage or places the sample of sample container
Product locker room 7 and refrigerant room 8 for accommodating liquid coolant and cryogenic gases, refrigerant room 8 is in sample storage room 7
Lower section；
At least one refrigerant input channel 9: for refrigerant to be filled into refrigerant room 8 by the outside of Dewar 1, this reality
It is a plurality of for applying refrigerant input channel 9 in example；
The closed barrier 10 of air-tightness: it is set to 1 inner cavity of Dewar between sample storage room 7 and refrigerant room 8, prevents sample
Product locker room 7 and refrigerant room 8 carry out gas exchanges；
At least one airway tube external 11: for directly arranging the gas in refrigerant room 8 without sample storage room 7
The outside of Dewar 1 out, airway tube external 11 is a plurality of in this embodiment, and the part of Dewar 1 is stretched out on airway tube external 11
It is provided with pressure relief valve 17, the part that Dewar 1 is stretched out on airway tube external 11 is additionally provided with exhaust branch pipe 18, exhaust branch pipe
18 are equipped with external aeration valve 19；
At least one internal ventilation conduit 12: for depositing the part liquid nitrogen cold air generated in refrigerant room 8 importing sample
Storage chamber 7, internal ventilation conduit 12 is a plurality of in this embodiment, true between the inside and outside wall that internal ventilation conduit 12 passes through Dewar 1
Absolutely empty 6.In the present embodiment, the caliber of internal ventilation conduit 12 is less than the caliber of airway tube external 11.It is interior in the embodiment
Portion's airway 12 and airway tube external 11 are arranged independently of each other.
When supplementing perfusion liquid nitrogen to refrigerant room 8, when external aeration valve 19 is opened, generated from refrigerant room 8
Most of liquid nitrogen cold air will be flowed to except Dewar 1 by airway tube external 11；When stopping is perfused in liquid nitrogen, external aeration valve
11 close, and all liquid nitrogen cold air generated from the liquid nitrogen vaporization of refrigerant room 8 can be flowed into sample by internal ventilation conduit 12
Product locker room 7 enters heat inside Dewar 1 to absorb from the external environment of Dewar 1, the use of liquid nitrogen is made to reach highest
Efficiency, if the pressure in refrigerant room 8 unexpectedly increases, pressure relief valve 17 will be opened, and pass through high-pressure liquid nitrogen cold air
The excessive release of airway tube external 11 is walked, and the harm of pipeline blockage and valve invalidation is protected from protection system.
Six schematic cross-sectional view of embodiment of constant low temperature storage system of the present invention as shown in Figure 7, comprising:
Dewar 1: including bottle body 2 and it is installed on the foam lid 3 of 2 upper end of bottle body, bottle body 2 is by inner wall 4 and the integrated structure of outer wall 5
At being vacuum space 6 between inner wall 4 and outer wall 5, the inner cavity of bottle body 2 is equipped with for sample storage or places the sample of sample container
Product locker room 7 and refrigerant room 8 for accommodating liquid coolant and cryogenic gases, refrigerant room 8 is in sample storage room 7
Lower section；
At least one refrigerant input channel 9: for refrigerant to be filled into refrigerant room 8 by the outside of Dewar 1, this reality
It is a plurality of for applying refrigerant input channel 9 in example；
The closed barrier 10 of air-tightness: it is set to 1 inner cavity of Dewar between sample storage room 7 and refrigerant room 8, prevents sample
Product locker room 7 and refrigerant room 8 carry out gas exchanges；
At least one airway tube external 11: for directly arranging the gas in refrigerant room 8 without sample storage room 7
The outside of Dewar 1 out, airway tube external 11 is a plurality of in this embodiment, and the part of Dewar 1 is stretched out on airway tube external 11
It is provided with pressure relief valve 17, the part that Dewar 1 is stretched out on airway tube external 11 is additionally provided with exhaust branch pipe 18, exhaust branch pipe
18 are equipped with external aeration valve 19；
At least one internal ventilation conduit 12: for depositing the part liquid nitrogen cold air generated in refrigerant room 8 importing sample
Storage chamber 7, internal ventilation conduit 12 is a plurality of in this embodiment.In the present embodiment, the caliber of internal ventilation conduit 12 is less than outside
The caliber of airway 11.In the embodiment, internal ventilation conduit 12 protrudes into sample storage room 7 by the interior cavity wall of Dewar 1, interior
Portion's airway 12 and airway tube external 11 are arranged independently of each other.
The use process of the present embodiment and above-described embodiment five is essentially identical, and details are not described herein again.
Seven schematic cross-sectional view of embodiment of constant low temperature storage system of the present invention as shown in Figure 8, comprising:
Dewar 1: including bottle body 2 and it is installed on the foam lid 3 of 2 upper end of bottle body, bottle body 2 is by inner wall 4 and the integrated structure of outer wall 5
At being vacuum space 6 between inner wall 4 and outer wall 5, the inner cavity of bottle body 2 is equipped with for sample storage or places the sample of sample container
Product locker room 7 and refrigerant room 8 for accommodating liquid coolant and cryogenic gases, refrigerant room 8 is in sample storage room 7
Lower section；
At least one refrigerant input channel 9: for refrigerant to be filled into refrigerant room 8 by the outside of Dewar 1, this reality
It is a plurality of for applying refrigerant input channel 9 in example；
The closed barrier 10 of air-tightness: it is set to 1 inner cavity of Dewar between sample storage room 7 and refrigerant room 8, prevents sample
Product locker room 7 and refrigerant room 8 carry out gas exchanges；
At least one airway tube external 11: for directly arranging the gas in refrigerant room 8 without sample storage room 7
The outside of Dewar 1 out, airway tube external 11 is a plurality of in this embodiment, and the part of Dewar 1 is stretched out on airway tube external 11
It is provided with pressure relief valve 17；
At least one internal ventilation conduit 12: for depositing the part liquid nitrogen cold air generated in refrigerant room 8 importing sample
Storage chamber 7, internal ventilation conduit 12 is a plurality of in this embodiment.In the present embodiment, the caliber of internal ventilation conduit 12 is less than outside
The caliber of airway 11.In the embodiment, internal ventilation conduit 12 protrudes into sample storage room 7 by the interior cavity wall of Dewar 1, interior
Portion's airway 12 and airway tube external 11 are arranged independently of each other.
The difference of the present embodiment and above-described embodiment six is: being only provided with a pressure on airway tube external 11 and releases
Valve 17 is put, the starting threshold value of the pressure relief valve 17 can be set by selection, supplement perfusion liquid nitrogen to refrigerant room 8
When, only the pressure rise of refrigerant room 8 when pressure relief valve 17 just open, when activated, it is outer that air-flow can be more prone to flow direction
Portion's airway 11 and pressure relief valve 17 rather than the restrictive internal ventilation conduit 12 of air-flow；It is not filled in liquid nitrogen
When note, the pressure inside refrigerant room 8 is low, and pressure relief valve 17 is closed, all liquid nitrogen cold air meetings come from refrigerant room 8
It is flowed into sample storage room 7 by internal ventilation conduit 11.
Eight schematic cross-sectional view of embodiment of constant low temperature storage system of the present invention as shown in Figure 9, comprising:
Dewar 1: including bottle body 2 and it is installed on the foam lid 3 of 2 upper end of bottle body, bottle body 2 is by inner wall 4 and the integrated structure of outer wall 5
At being vacuum space 6 between inner wall 4 and outer wall 5, the inner cavity of bottle body 2 is equipped with for sample storage or places the sample of sample container
Product locker room 7 and refrigerant room 8 for accommodating liquid coolant and cryogenic gases, refrigerant room 8 is in sample storage room 7
Lower section；
At least one refrigerant input channel 9: for refrigerant to be filled into refrigerant room 8 by the outside of Dewar 1, this reality
It is a plurality of for applying refrigerant input channel 9 in example；
The closed barrier 10 of air-tightness: it is set to 1 inner cavity of Dewar between sample storage room 7 and refrigerant room 8, prevents sample
Product locker room 7 and refrigerant room 8 carry out gas exchanges；
At least one airway tube external 11: for directly arranging the gas in refrigerant room 8 without sample storage room 7
The outside of Dewar 1 out, airway tube external 11 is a plurality of in this embodiment, and the part of Dewar 1 is stretched out on airway tube external 11
It is provided with pressure relief valve 17, the part that Dewar 1 is stretched out on airway tube external 11 is additionally provided with exhaust branch pipe 18, exhaust branch pipe
18 are equipped with external aeration valve 19；
At least one internal ventilation conduit 12: for depositing the part liquid nitrogen cold air generated in refrigerant room 8 importing sample
Storage chamber 7, internal ventilation conduit 12 is a plurality of in this embodiment.In the present embodiment, the caliber of internal ventilation conduit 12 is less than outside
The caliber of airway 11.In the embodiment, internal ventilation conduit 12 and airway tube external 11 are arranged independently of each other.
In the present embodiment, be provided at least one thermal insulation cabinet 20 in sample storage room 7, sample storage in thermal insulation cabinet 20,
Thermal insulation cabinet 20 includes the cabinet body 21 made of heat-transfer metal material, and cabinet body 21 is divided the inner cavity of cabinet body 21 by multiple partitions 22
At multiple chambers, partition 22 is made of insulating heat conduction holes metal material.The upper and lower surfaces of cabinet body 21 are respectively arranged with multi-layer heat-transfer
Insulating layer 23, the present embodiment heat transfer insulating layer 23 are made of a foam material.The inner chamber bottom surface of Dewar 1 is placed with support platform
24, which is also to be made of heat conducting material.The upper surface of support platform 24 is located at below closed barrier 10.
Use process basic phase of the present embodiment when supplementing perfusion refrigerant to refrigerant room 8 and when not needing perfusion
Together, and advantage is also essentially identical, unlike: when the foam lid 3 of Dewar 1 is opened, the liquid nitrogen that is generated by refrigerant room 8
Cold airflow can also remove the thick fog in refrigerant room 8, to improve visibility, and maintain the low temperature of sample storage room 7 simultaneously.
As shown in Figure 10, Figure 11, nine structure of embodiment of constant low temperature storage system of the present invention includes:
Dewar 1: including bottle body 2 and it is installed on the foam lid 3 of 2 upper end of bottle body, bottle body 2 is by inner wall 4 and the integrated structure of outer wall 5
At being vacuum space 6 between inner wall 4 and outer wall 5, the inner cavity of bottle body 2 is equipped with for sample storage or places the sample of sample container
Product locker room 7 and refrigerant room 8 for accommodating liquid coolant and cryogenic gases, refrigerant room 8 is in sample storage room 7
Lower section；
At least one refrigerant input channel 9: for refrigerant to be filled into refrigerant room 8 by the outside of Dewar 1, this reality
It is a plurality of for applying refrigerant input channel 9 in example, naturally it is also possible to be one, be the scope of protection of the invention；
The closed barrier 10 of air-tightness: it is set to 1 inner cavity of Dewar between sample storage room 7 and refrigerant room 8, prevents sample
Product locker room 7 and refrigerant room 8 carry out gas exchanges；
At least one airway tube external 11: for directly arranging the gas in refrigerant room 8 without sample storage room 7
The outside of Dewar 1 out, airway tube external 11 is a plurality of in this embodiment；
At least one internal ventilation conduit 12: for depositing the part liquid nitrogen cold air generated in refrigerant room 8 importing sample
Storage chamber 7, internal ventilation conduit 12 is a plurality of in this embodiment, true between the inside and outside wall that internal ventilation conduit 12 passes through Dewar 1
Absolutely empty 6.In the present embodiment, the caliber of internal ventilation conduit 12 is less than the caliber of airway tube external 11.It is interior in the embodiment
Portion's airway 12 and airway tube external 11 are arranged independently of each other；
The present embodiment further includes the level gauging pipe 25 for measuring cryodose, and one end of level gauging pipe 25 is protruded into cold
Freeze in the refrigerant of agent room 8, the other end of level gauging pipe 25 connects liquid level sensor 26, liquid level sensor after stretching out Dewar 1
26 connect with refrigerant fluid infusion control system；
In the present embodiment, the bottom surface of Dewar 1 is connected with multiple support legs 27, and the present embodiment is four, each support leg 27
Lower end is separately installed with idler wheel 28.
The use process and advantage of the present embodiment are most of identical as embodiment described in Fig. 6, are a difference in that: passing through setting
Level gauging pipe 25 and liquid level sensor 26 can be convenient user and detected by the level gauging pipe 25 that liquid level sensor 26 provides
Cryodose, at any time to refrigerant room 8 supplement refrigerant, the method for supplementing refrigerant can be automatic control refrigerant is mended
Enter refrigerant room 8, refrigerant manually can also be filled into refrigerant room 8, guarantee the stability of sample storage temperature.
By the way that dry filter air is inversely pressed into the storage of the described in any item constant low temperatures of above-described embodiment 2- embodiment 9
System can remove the fog in Dewar 1, basic operation method are as follows: by dry filter air by constant low temperature storage system
In airway tube external 11 be pressed into refrigerant room 8 in, play dry filter air in the liquid coolant in refrigerant room 8
Bubble is flowed into demisting in sample storage room 7 by internal ventilation conduit 12 later.
Now in conjunction with embodiment described in Fig. 4, the method for the fog in removal Dewar 1 is specifically introduced:
(1) dry filter air is injected into airway tube external 11, opens control 11 on-off of airway tube external
External aeration valve 19 communicates airway tube external 11 with refrigerant room 8；
(2) the dry filter air injected blisters in the liquid coolant in refrigerant room 8, and it is internal logical to open control
The internal ventilation valve 16 of 12 on-off of airway, chilled dose of room 8 of dry filter air, internal ventilation conduit 12 are flowed into sample
In locker room 7.
Pass through the method for above-mentioned removal Dewar fog, it is possible to provide sample storage room 7 is washed away with dry filter air, to reach
Drive away the purpose of fog.
According to same principle, after dry filter air is imported into refrigerant room 8 by refrigerant input channel 9, open
The internal ventilation valve 16(of control 12 on-off of internal ventilation conduit, into Figure 11, is not provided with this valve in Fig. 5), dry filter
Chilled dose of room 8 of air, internal ventilation conduit 12, which are flowed into, can achieve the purpose for being rapidly frozen sample in sample storage room 7.
Although having used liquid nitrogen and liquid nitrogen gas as application example, in the description of the present invention with main idea of the present invention
Speech, in the description of the present invention, is equally applicable to other types of refrigerant and its gas.
Those skilled in the art after considering the specification and implementing the invention disclosed here, will readily occur to of the invention its
Its embodiment.This application is intended to cover any variations, uses, or adaptations of the invention, these modifications, purposes or
Person's adaptive change follows general principle of the invention and including undocumented common knowledge in the art or usual
Technological means.The description and examples are only to be considered as illustrative, and true scope and spirit of the invention are wanted by following right
It asks and points out.
It should be understood that the present invention is not limited to the precise structure already described above and shown in the accompanying drawings, and
And various modifications and changes may be made without departing from the scope thereof.The scope of the present invention is limited only by the attached claims.
Claims (10)
1. a kind of constant low temperature storage system, comprising:
Dewar: be equipped with for sample storage or place in it sample container sample storage room and for accommodate liquid coolant and
The refrigerant room of cryogenic gases；
At least one refrigerant input channel: for by refrigerant by being filled into the refrigerant room outside Dewar；
It is characterized by further comprising:
Closed barrier: being set between the sample storage room and refrigerant room, prevents the two from carrying out gas exchanges；
At least one airway tube external: for the gas of the refrigerant room to be expelled directly out without the sample storage room
Outside Dewar；
Further include: at least one internal ventilation conduit: deposited for the indoor part cold air of the refrigerant to be imported the sample
Storage chamber；
The caliber of the internal ventilation conduit is less than the caliber of airway tube external, and the cold air from the refrigerant room is made to exist
It is preferentially exported except the Dewar by the airway tube external when supplement perfusion refrigerant, to guarantee the sample storage
Chamber interior temperature it is constant.
2. constant low temperature storage system according to claim 1, which is characterized in that be provided on the internal ventilation conduit
Internal ventilation valve.
3. constant low temperature storage system according to claim 1, which is characterized in that the internal ventilation conduit and described outer
The connection of portion's airway.
4. constant low temperature storage system according to claim 1, which is characterized in that the internal ventilation conduit is set to Du
Watt inside and outside wall between or be set to the internal chamber wall of Dewar.
5. constant low temperature storage system according to claim 1-4, which is characterized in that the airway tube external
The part for stretching out Dewar is provided with pressure relief valve.
6. constant low temperature storage system according to claim 5, which is characterized in that stretch out Du on the airway tube external
Watt part be provided with exhaust branch pipe, external aeration valve is provided on the exhaust branch pipe.
7. constant low temperature storage system according to claim 6, which is characterized in that be provided in the sample storage room to
A few thermal insulation cabinet, for the sample storage in the thermal insulation cabinet, the thermal insulation cabinet includes made of heat-transfer metal material
Cabinet body, the cabinet body include at least one chamber, and the upper and lower surfaces of the cabinet body are respectively arranged with multi-layer heat-transfer insulating layer.
8. according to claim 1,2,3,4,6,7 described in any item constant low temperature storage systems, which is characterized in that the Dewar
Inner chamber bottom surface be placed with the support platform made of heat conducting material, the upper surface of the support platform is located at the closed resistance
Below.
9. constant low temperature storage system according to claim 6, which is characterized in that further include for measuring cryodose
Refrigerant is protruded into level gauging pipe, one end of the level gauging pipe, and the other end of the level gauging pipe connects after stretching out Dewar
Connect liquid level sensor.
10. a kind of method for removing Dewar fog, which is characterized in that by dry filter air by any one of claim 1-9 institute
The airway tube external in constant low temperature storage system and/or refrigerant input channel stated are pressed into the refrigerant room, are made
Dry filter air blisters in the liquid coolant in refrigerant room, is flowed into sample storage by internal ventilation conduit later
Demisting and/or quickening sample freezing in room.
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| CN110168274A (en) * | 2017-10-26 | 2019-08-23 | 二十一世纪药学股份有限公司 | Cryogenic storage system with improved temperature stability |
| EP3924675B1 (en) * | 2019-02-17 | 2023-07-05 | National Scientific And Technical Research Council - Argentina (Conicet) | System for accessing biological samples in a cryogenic dewar vessel |
| CN110742057B (en) * | 2019-10-09 | 2021-10-29 | 北京润美玉之光医疗美容门诊部 | Low-temperature preservation method for autologous fat particles |
| CN115930095B (en) * | 2022-12-23 | 2024-04-23 | 成都西南交通大学设计研究院有限公司 | Liquid nitrogen filling device and method for magnetic levitation train |
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Effective date of registration: 20190118 Address after: Room 019, Beijing International Medical Service Area, No. 1 Canal East Street, Tongzhou District, Beijing Applicant after: Beijing Huachen Century Biotechnology Co., Ltd. Address before: Hongkong, China Applicant before: Cai Chenyu |
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Inventor after: Cai Chen Yu Inventor after: John Dean Barry Inventor after: Brian Gregory Walker Inventor after: Limdo Zhou Inventor after: Yang Zhen Ling Inventor after: Deng Ke Jia Inventor before: Cai Chenyu |
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Address after: 100012 Room 101, floor 1-5, building 351, Shuian manor, Chaoyang District, Beijing (04) 401058 Patentee after: Linghang Zhifei management consulting (Beijing) Co.,Ltd. Address before: 101149 room 019, Beijing international medical service area, No.1 Yunhe East Street, Tongzhou District, Beijing Patentee before: Beijing Huachen Century Biotechnology Co.,Ltd. |