CN106631953A - Preparation method of beta-carotene in paprika - Google Patents

Abstract

The invention belongs to the technical field of food, in particular to a preparation method of β-carotene in pepper. The extraction method comprises: drying, pulverizing, granulating and extracting the peppers to obtain a pigment leachate; after despicing the pigment leachate, adopting a wet method to continuously load samples for chromatographic separation to obtain two eluents respectively. The eluate is concentrated to obtain capsanthin and capsanthin; the capsanthin is saponified, precipitated, filtered, and vacuum-dried to obtain β-carotene products. The present invention uses capsicum as a raw material for the first time to prepare natural β-carotene. On the basis of separation of red and yellow, it not only makes up for the defect in the single development of capsicum products that use sweet peppers as raw materials, but also improves the utilization value of the pigment of capsanthin. It has opened up a new direction for the development of pepper industry.

Description

A kind of preparation method of β-carotene in capsicum

technical field

The invention belongs to the technical field of food additives, and in particular relates to a preparation method of beta-carotene in pepper.

Background technique

Paprika red pigment is a natural red pigment extracted from the peel of ripe red pepper, and its main components are capsanthin, capsorubin, β-carotene and Zeaxanthin, etc., whose color intensity is 10 times that of other pigments, is widely used in food, beverage, cosmetics and pharmaceutical industries, and is internationally recognized as the best class A red pigment. Capsanthin is the bright red part of red peppers. However, because it is mostly used in food and other fields, its added value is too low, which will eventually affect the long-term development of the entire red pepper industry and the pepper industry.

The main preparation process of traditional capsanthin: dry and pulverize capsicum, use solvent extraction method to obtain the crude extract of capsanthin, then use lye or ethanol and other solvents to extract and remove spicy substances, and distill out the Solvent to obtain refined capsanthin.

The main development direction of the existing peppers are capsanthin and capsicum oleoresin, which are mainly used in beverages, cold drinks, baking and other food fields, as well as beverages, cosmetics and other fields. Capsanthin itself contains a variety of active substances, such as capsanthin, capsanthin, β-carotene and so on. However, at present, reddish capsanthin has a wide range of application fields, and the development of capsicum yellow pigment capsicum products is not deep enough to reflect the value of β-carotene, zeaxanthin and lutein, etc. The yellow-red separation cost of capsanthin is too high, but its corresponding economic value cannot be received.

β-carotene is one of the carotenoids, and it is also an orange-yellow fat-soluble compound. It is the most common natural pigment in nature, such as green vegetables, sweet potatoes, carrots, peppers, salina, etc. are rich in β-carotene .

β-carotene is one of the carotenoids. Carotene has three isomers of α, β, and γ, and the content of β-isomer accounts for more than 80%. β-carotene, also known as β-carotene, has the following structural formula:

Its molecular formula is C ₄₀ H ₅₆ , and its molecular weight is 536.88. β-carotene has various cis-trans isomers, such as all-trans-β-carotene, 9-cis-β-carotene, 15-cis-β-carotene, etc. Natural β-carotene is a mixed configuration of trans and cis, and synthetic β-carotene is an all-trans configuration. But from the perspective of molecular structure, there is no difference between natural β-carotene and synthetic β-carotene, and there is no difference in chemical and physical properties.

β-carotene is currently widely used in food, feed additives, and cosmetic additives. Recent studies have shown that: β-carotene has antioxidant and photoprotective effects. People who often take in rich carotene in their diet have significantly lower chances of suffering from diseases such as arteriosclerosis, certain cancers, and degenerative eye diseases than those who take in less carotene. Many animal experiments have also proved this point of view. For example, the vision of the eye depends on the macula in the fundus. If there is not enough β-carotene for protection and support, degenerative lesions will occur in this part, that is, aging, vision will decline and even night blindness will eventually occur. This disease mostly occurs in the elderly. Although the medical profession believes that it is a manifestation of aging, it also points out that this degenerative eye disease can be prevented by taking enough β-carotene. This major discovery gave people a new understanding of carotene, and believed that it is not only a necessary substance for achieving balanced nutrition, but also helps people prevent diseases, prolong life, and improve physical fitness and quality of life.

In addition, a large number of scientific research data at home and abroad have confirmed that β-carotene has definite curative effect on cancer prevention and treatment. The flood of free oxygen in the body will not only damage normal cells, but also often cause distortion and form cancer. β-carotene is precisely the strongest "nemesis" of oxygen free radicals. Scientific research has confirmed that the β-carotene in the blood of cancer patients is far lower than that of normal people. When cancer patients receive radiotherapy and chemotherapy, β-carotene can reduce their toxic and side effects. Radiotherapy can induce the production of oxygen free radicals, and the integrity of cell microsomal membranes can prevent the production of oxygen free radicals, and β-carotene can maintain the integrity of microsomal membranes; chemotherapy drugs can cause mutations in normal cells while killing cancer cells, while β-carotene can -Carotene has an anti-mutagenic effect, thereby reducing its toxic and side effects.

At present, there are three main sources of β-carotene: synthesis, microbial fermentation and natural plant extraction. However, with the continuous improvement of people's health concept, the pursuit of natural has become a trend, and the demand for natural β-carotene is also increasing. However, there are not many plant resources that can provide enough raw materials for the extraction of β-carotene, which has become a bottleneck for the industrial production of naturally extracted β-carotene.

Contents of the invention

In order to overcome the deficiencies described in the background technology, the present invention provides a preparation method of β-carotene in capsicum. For the first time, capsicum is used as a raw material to prepare natural β-carotene. It is the defect of the unitary development of the pepper product as raw material, and improves the utilization value of the pigment of capsanthin, and opens up a new direction for the industrialization development of pepper.

The technical solution adopted by the present invention to solve its technical problems is:

A preparation method of beta-carotene in capsicum, comprising the following steps:

(1) Preparation of raw materials: drying mature peppers at 30-50° C., pulverizing and granulating to obtain pepper granules;

(2) Extraction of capsanthin: transport the capsicum granules into the continuous extractor, spray the extraction solvent through the spray pipe during the transportation process, so that the extraction agent and the capsicum granules are evenly mixed, and the capsanthin is extracted by the extraction solvent. Leach out to obtain the pigment leachate;

(3) Despicy treatment: mix the pigment extract and alcohol solvent in a liquid-liquid continuous extractor to remove the spicy substances to obtain the despiced pigment and despicy extract;

(4) Removal of alcohol solvent residues: mixing the pigment depigmentation extract and saturated saline in a liquid-liquid continuous extractor to remove alcohol solvent residues;

(5) Chromatographic separation: a) the pigment leachate obtained in step (4) is directly applied to a silica gel column for adsorption and separation, and the port effluent is collected; For elution, the eluate is combined with the port effluent to obtain a capsanthin eluate, and the capsanthin eluate is concentrated to obtain capsanthin; c) eluting the silica gel column with acetone to obtain an acetone eluate , concentrating the acetone eluent to obtain capsanthin;

(6) Saponification refining: a) mix capsicum yellow with alkaline alcohol solution, and carry out saponification reaction under water bath stirring condition; b) after saponification, use acidic solution to adjust pH to 4.0-9.0, use alcohol solvent in Dilution treatment is carried out under the condition of stirring in a water bath, standing for crystallization, and filtering to obtain the saponification filtrate and crude β-carotene; c) washing the crude β-carotene with an alcohol solvent, filtering to obtain a filter cake and a crude washing solution, and filtering The cake is the fine β-carotene, and the fine β-carotene is dried under reduced pressure to obtain the fine β-carotene; d) The saponification filtrate is combined with the crude product washing liquid, concentrated to the residue of absolute ethanol, and a paste-like by-product is obtained;

(7) Extraction of by-products: dissolving the paste-like by-products in water, and extracting with an organic solvent, standing, and layering, the upper layer solution is concentrated and recovered to obtain a paste-like pigment product; the lower layer is processed by removing the organic solvent, The powder capsicum yellow product is obtained by spray drying.

Preferably, in step (2), the continuous extractor is a horizontal extractor, an annular extractor, a satellite extractor or a crawler extractor; the extraction solvent is a vegetable oil extraction solvent, and the standard for the vegetable oil extraction solvent is in accordance with GB 16629-2008 Vegetable Oil Extraction Solvent; the spray temperature of the spray pipe is 40-50°C, and the spray volume is 1000-1500L/h.

Preferably, in step (3), the alcoholic solvent is one of methanol, ethanol, propanol, isopropanol or butanol; the temperature of the reaction system is 30-50°C; the flow rate ^of the pigment leachate is 0.5-1.5m3 /h, the flow rate of the alcohol solvent is 1-3m ³ /h; the upper layer in the liquid-liquid continuous extractor is the pigment leachate, the lower layer is the alcohol solvent, and the alcohol solvent extracts capsaicin, dihydrocapsaicin and Pungent substances such as nordihydrocapsaicin are removed to obtain a despiced pigment despiced extract.

Preferably, in step (4), the flow rate of the pigment despicing leachate is 1-3m ³ /h, the flow rate of saturated saline is 0.5-1.5m ³ /h, the upper layer in the liquid-liquid continuous extractor is the pigment despicing leachate, the lower layer It is saturated saline, and the saturated saline extracts the residual alcohol solvent in the leachate of pigment despicing.

Preferably, in step (5), the eluent used in step b) is a vegetable oil extraction solvent, the vegetable oil extraction solvent elution rate is 0.2-2Bv/h, and the vegetable oil extraction solvent and silica gel column volume ratio is 2- 4:1; the concentration method used in step b) is vacuum concentration, the pressure is -0.03～-0.06Mpa, the concentration temperature is 40-100°C; the volume ratio of acetone to silica gel in step c) is 2-4:1 , Acetone elution rate is 0.2-2Bv/h.

Preferably, in step (6), the alkaline alcohol solution in step a) is an alcohol solution of KOH, the water bath temperature is 40-50°C, the stirring condition is 150-200r/min, and the saponification reaction temperature is 50-60°C, The saponification time is 2.5-3h; the acidic solution in step b) is hydrochloric acid, the alcoholic solvent is ethanol with a concentration of 60-70%, the water bath temperature is 40-50°C, the stirring condition is 150-200r/min, and the crystallization temperature is 40 DEG C, filter cloth pore diameter is 500 meshes during filtration; The alcoholic solvent that adopts in the step c) is the ethanol of concentration 60-70%, the vacuum degree of decompression drying is-0.08～-0.09MPa, and decompression drying temperature is 45 -50°C; step d) adopts the method of concentrating under reduced pressure, the concentration temperature is 70-75°C, and the vacuum degree of concentration is -0.06～-0.08Mpa.

Preferably, in step (7), the ratio of by-products to water is 1:1, the organic solvent is ethyl acetate, the ratio of by-product solution and organic solvent is 1:1, the spray drying inlet temperature is 185-195°C, and the spray drying The outlet temperature is 85-90°C.

The advantages of the present invention are: for the first time, capsicum is used as raw material to prepare natural β-carotene. On the basis of separation of red and yellow, it not only makes up for the defect of single development of capsicum products with sweet pepper as raw material, but also improves the pigment of capsanthin The utilization value has opened up a new direction for the development of pepper industry.

detailed description

The present invention will be further described below in conjunction with embodiment:

Example 1

a) Take 10t of mature peppers, dry them, crush them and granulate them, and extract them with a flat-rotating continuous extractor to obtain a pigment extract. Mix the pigment leachate with 70% ethanol in a liquid-liquid extractor at 45°C and 200r/min to remove the spicy substance, the flow rate of the pigment leachate is 1m ³ /h, and the flow rate of 70% ethanol is 2m ³ /h , to get off the peppery pigment spicy leachate. Mix the depigmented spicy extract with saturated saline at ^200r /min to remove residual ethanol in the depigmented extract. 0.6m ³ /h to obtain a despiced pigment extract.

b) The pigment leachate from which residual ethanol has been removed is subjected to silica gel column chromatography at a flow rate of 1 Bv/h by continuous wet loading, and the effluent from the lower port is collected. First use 5000L vegetable oil extraction solvent to elute at a flow rate of 0.3Bv/h, and combine the eluate with the liquid collected from the lower port to obtain the capsanthin eluate. The capsanthin eluate was concentrated under reduced pressure to obtain 201.6 Kg of dark yellow capsanthin with a color value of 173.5 and A ₄₇₀ /A ₄₅₄ of 0.848. Then, 7000 L of acetone was used to elute the silica gel column at a flow rate of 0.3 Bv/h to obtain an acetone eluate. The acetone eluate was concentrated under reduced pressure to obtain 298.7 Kg of deep red capsanthin with a color value of 338.7 and A ₄₇₀ /A ₄₅₄ of 1.024.

c) Take 200Kg of capsicum yellow pigment and place it in a saponification reaction tank, add 240Kg of potassium hydroxide-70% ethanol (concentration of potassium hydroxide is 20%) under the stirring condition of 150r/min, and saponify in a 60°C water bath for 3h. After the reaction is completed, adjust the pH to 7.5 with 10% hydrochloric acid, then add 480Kg of 70% ethanol to the reaction solution, and stir at 40°C and 150r/min for 30min. After stopping the stirring, pass through a 400-mesh filter cloth while it is hot, collect the filter cake, and obtain the crude product of β-carotene. Add 200Kg of 70% ethanol to the filter cake, and stir for 30min at 40°C and 150r/min. After stopping stirring, pass through a 400-mesh filter cloth while hot to collect the filter cake. The filter cake was vacuum-dried at 55°C and -0.09Mpa for 15 hours to obtain 15.1Kg of refined β-carotene with a color value of 1084.6 and A ₄₇₀ /A ₄₅₄ of 0.868. Combine the two filtrates, concentrate under reduced pressure at 70°C and -0.08Mpa to recover ethanol-free, add 150Kg ethyl acetate, and stir at room temperature and 150r/min for 20min. After stopping stirring, let the layers stand for 1h. Take the lower layer and recover the residual ethyl acetate under the conditions of 60°C and -0.06Mpa to obtain a pigment aqueous solution, add 10Kg of maltodextrin and mix well, then spray dry at an inlet temperature of 190°C and an outlet temperature of 90°C to obtain a powder The body pepper yellow product is 148Kg, and the color value is 23.8. The ethyl acetate solution in the upper layer was concentrated at 50°C and -0.06Mpa to recover the solvent to obtain 89.7Kg of oily capsanthin with a color value of 150.9 and A ₄₇₀ /A ₄₅₄ of 0.862.

Example 2

a) Take 10t of mature peppers, dry them, crush them and granulate them, and extract them with a flat-rotating continuous extractor to obtain a pigment extract. Mix the pigment extract and 70% ethanol in a liquid ^- liquid extractor at 50°C and ^200r /min to remove the spicy substances. h, to obtain the hot-pigmented spicy leachate. Mix the ^despiced pigmented leachate with saturated saline at 200r/min to remove the residual ethanol in the depigmented extract. m ³ /h to obtain a despiced pigment extract.

b) The pigment leachate from which residual ethanol has been removed is subjected to silica gel column chromatography at a flow rate of 0.8 Bv/h by continuous wet loading, and the effluent from the lower port is collected. First use 6500L vegetable oil extraction solvent to elute at a flow rate of 0.5Bv/h, and combine the eluate with the liquid collected from the lower port to obtain the capsanthin eluate. The capsanthin eluate was concentrated under reduced pressure to obtain 178Kg of dark yellow capsanthin with a color value of 186.8 and A ₄₇₀ /A ₄₅₄ of 0.862. Then, 7800 L of acetone was used to elute the silica gel column at a flow rate of 0.5 Bv/h to obtain an acetone eluate. The acetone eluate was concentrated under reduced pressure to obtain 279.8 Kg of deep red capsanthin with a color value of 344.6 and A ₄₇₀ /A ₄₅₄ of 1.023.

c) Take 175Kg of capsicum yellow pigment and place it in a saponification reaction tank, add 140Kg of potassium hydroxide-80% ethanol (concentration of potassium hydroxide is 30%) under stirring condition of 150r/min, and saponify in a 60°C water bath for 3h. After the reaction is completed, adjust the pH to 6.2 with 10% hydrochloric acid, then add 300Kg of 80% ethanol to the reaction solution, and stir at 40°C and 150r/min for 30min. After stopping the stirring, pass through a 500-mesh filter cloth while it is hot, collect the filter cake, and obtain the crude product of β-carotene. Add 150Kg of 80% ethanol to the filter cake, and stir for 30min at 40°C and 150r/min. After stopping stirring, pass through a 500-mesh filter cloth while hot to collect the filter cake. The filter cake was vacuum-dried at 50°C and -0.09Mpa for 12 hours to obtain 13.8Kg of refined β-carotene with a color value of 1206.4 and A ₄₇₀ /A ₄₅₄ of 0.872. Combine the two filtrates, concentrate under reduced pressure at 70°C and -0.08Mpa to recover ethanol-free, add 100Kg ethyl acetate, and stir at room temperature and 150r/min for 30min. After stopping stirring, let the layers stand for 1h. Take the lower layer liquid and recover residual ethyl acetate under the condition of 50°C and -0.06Mpa to obtain a pigment aqueous solution, add 10Kg of maltodextrin and mix well, and spray dry at an inlet temperature of 180°C and an outlet temperature of 80°C to obtain powdered pepper The yellow product weighs 136.8Kg and has a color value of 25.6. The ethyl acetate solution in the upper layer was concentrated at 50°C and -0.06Mpa to recover the solvent to obtain 73.8Kg of capsanthin in the form of ointment, with a color value of 153.7 and A ₄₇₀ /A ₄₅₄ of 0.844.

Example 3

a) Take 10t of mature peppers, dry them, crush them and granulate them, and extract them with a flat-rotating continuous extractor to obtain a pigment extract. Mix the pigment extract and 70% ethanol in a liquid ^- liquid extractor at 50°C and ^200r /min to remove the spicy substances. h, to obtain the hot-pigmented spicy leachate. Mix the ^despiced pigmented leachate with saturated saline at 200r/min to remove the residual ethanol in the depigmented extract. m ³ /h to obtain a despiced pigment extract.

b) The pigment leachate from which residual ethanol has been removed is subjected to silica gel column chromatography at a flow rate of 0.8 Bv/h by continuous wet loading, and the effluent from the lower port is collected. First use 5000L vegetable oil extraction solvent to elute at a flow rate of 0.5Bv/h, and combine the eluate with the liquid collected from the lower port to obtain the capsanthin eluate. The capsanthin eluate was concentrated under reduced pressure to obtain 214.3 Kg of dark yellow capsanthin with a color value of 169.2 and A ₄₇₀ /A ₄₅₄ of 0.865. Then, 7200 L of acetone was used to elute the silica gel column at a flow rate of 0.5 Bv/h to obtain an acetone eluent. The acetone eluate was concentrated under reduced pressure to obtain 304.2 Kg of deep red capsanthin with a color value of 327.3 and A ₄₇₀ /A ₄₅₄ of 1.027.

c) Take 210Kg of capsicum yellow pigment and place it in a saponification reaction tank, add 130Kg of potassium hydroxide-70% ethanol (concentration of potassium hydroxide is 40%) under stirring condition of 150r/min, and saponify in a 50°C water bath for 3h. After the reaction is completed, adjust the pH to 5.0 with 10% hydrochloric acid, then add 400Kg of 70% ethanol to the reaction solution, and stir at 50°C and 150r/min for 20min. After stopping the stirring, pass through a 500-mesh filter cloth while it is hot, collect the filter cake, and obtain the crude product of β-carotene. Add 200Kg of 70% ethanol to the filter cake, and stir for 30min at 50°C and 150r/min. After stopping stirring, pass through a 400-mesh filter cloth while hot to collect the filter cake. The filter cake was vacuum-dried at 60°C and -0.09Mpa for 12 hours to obtain 13.8Kg of fine β-carotene with a color value of 1394.1 and A ₄₇₀ /A ₄₅₄ of 0.865. Combine the two filtrates, concentrate under reduced pressure at 70°C and -0.08Mpa to recover ethanol-free, add 120Kg ethyl acetate, and stir at room temperature and 150r/min for 40min. After stopping stirring, let the layers stand for 1.5h. Take the lower layer and recover the residual ethyl acetate under the condition of 70°C and -0.07Mpa to obtain a pigment aqueous solution, add 8Kg of maltodextrin, and spray dry at an inlet temperature of 190°C and an outlet temperature of 90°C to obtain a powdered capsicum yellow product 157.4Kg, color value is 20.3. The ethyl acetate solution in the upper layer was concentrated at 60°C and -0.04Mpa to recover the solvent to obtain 73.2Kg of capsanthin in ointment form, with a color value of 155.2 and A ₄₇₀ /A ₄₅₄ of 0.868.

The content of β-carotene in the β-carotene products prepared in the above examples was detected. The test results are shown in Table 1.

Table 1 embodiment β-carotene product component detection result

Example 1 Example 2 Example 3 color price 1084.6 1206.4 1394.1 Absorbance ratio 0.868 0.872 0.865 β-carotene/% 32.4 38.5 41.9

Finally, it should be noted that obviously, the above-mentioned embodiments are only examples for clearly illustrating the present invention, rather than limiting the implementation. For those of ordinary skill in the art, on the basis of the above description, other changes or changes in different forms can also be made. It is not necessary and impossible to exhaustively list all the implementation manners here. However, the obvious changes or variations derived therefrom are still within the protection scope of the present invention.

Claims (7)

1. in a kind of Fructus Capsici beta-carotene preparation method, it is characterised in that described preparation method is comprised the following steps：

(1) preparing raw material：By ripe Fructus Capsici in 30-50 DEG C of drying, crush, pelletize obtains Fructus Capsici granule；

(2) capsicum red pigment is extracted：Fructus Capsici granule is delivered into inside continuous way infuser, by spray in course of conveying Shower pipe spraying and extracting solvent, makes extractant mix homogeneously with Fructus Capsici granule, is leached out capsicum red pigment by extractant, Obtain pigment leachate；

(3) peppery process is taken off：Pigment leachate is mixed with alcohols solvent in liquid-liquid continuous extraction device, spicy substance is sloughed, is obtained Peppery leachate is taken off to de- peppery pigment；

(4) alcohols solvent residual is sloughed：Pigment is taken off into peppery leachate and saturated aqueous common salt to be mixed in liquid-liquid continuous extraction device Row sloughs alcohols solvent residual treatment；

(5) chromatography：A) the pigment leachate that step (4) is obtained directly is gone up into silicagel column carries out adsorbing separation, and collecting terminal Mouth effluent；B) after silicagel column adsorption saturation, eluting, eluent and port flow are carried out to silicagel column using alkanes eluant Go out liquid and merge to obtain Fructus Capsici flavochrome eluent, Fructus Capsici flavochrome eluent is carried out being concentrated to give Fructus Capsici flavochrome；C) with acetone pair Silicagel column carries out eluting, obtains acetone eluant, by acetone eluant concentration, obtains capsicum red pigment；

(6) saponification is refined：A) Fructus Capsici flavochrome is mixed with alkaline alcohol solution, saponification is carried out under the conditions of stirring in water bath anti- Should；B) after saponification is finished, pH to 4.0-9.0 is adjusted using acid solution, is carried out under the conditions of stirring in water bath using alcohols solvent Dilution is processed, and stands crystallize, is filtered, and obtains saponification filtrate and beta-carotene crude product；C) using alcohols solvent to beta-carotene Crude product is scrubbed, is filtrated to get filter cake and crude product cleaning mixture, and filter cake is beta-carotene fine work, drying under reduced pressure beta-carotene essence Product obtain beta-carotene fine work；D) saponification filtrate is merged with crude product cleaning mixture, is concentrated into dehydrated alcohol residual, obtain cream Shape by-product；

(7) by-product is extracted：By paste by-product water dissolution, and extracted with organic solvent, stood, be layered, upper strata is molten The concentrated recycling design of liquid, obtains ointment shape pigment product；Lower floor obtains powder Jing after removing organic solvent is processed using spray drying Body Fructus Capsici Huang product.

2. in a kind of Fructus Capsici as claimed in claim 1 beta-carotene preparation method, it is characterised in that in step (2), even Continuous formula infuser is flat rotary extractor, loop type extractor, satellite type infusion device or crawler type infuser；Extractant is plant Oil extraction solvent；Shower spray temperature is 40-50 DEG C, and spray flux is 1000-1500L/h.

3. in a kind of Fructus Capsici as claimed in claim 2 beta-carotene preparation method, it is characterised in that in step (3), alcohol Class solvent is the one kind in methanol, ethanol, propanol, isopropanol or butanol；The temperature of reaction system is 30-50 DEG C；Pigment is leached Flow quantity is 0.5-1.5m³/ h, the flow of alcohols solvent is 1-3m³/h；Liquid-liquid continuous extraction device is at the middle and upper levels pigment leachate, Lower floor is alcohols solvent, the pungent thing such as capsaicin, dihydro Capsaicin and drop dihydro Capsaicin in alcohols solvent extraction pigment leachate Matter, after removing spicy substance, obtains de- peppery pigment and takes off peppery leachate.

4. in a kind of Fructus Capsici as claimed in claim 3 beta-carotene preparation method, it is characterised in that in step (4), color The de- peppery leaching flow quantity of element is 1-3m³/ h, saturated common salt discharge is 0.5-1.5m³/ h, liquid-liquid continuous extraction device is at the middle and upper levels Pigment takes off peppery leachate, and lower floor is saturated aqueous common salt, and saturated aqueous common salt extraction pigment takes off the alcohols solvent remained in peppery leachate.

5. in a kind of Fructus Capsici as claimed in claim 4 beta-carotene preparation method, it is characterised in that in step (5), step It is rapid b) in eluant be vegetable oil extraction solvent, vegetable oil extraction solvent elution speed is 0.2-2Bv/h, plant oil extraction Solvent is 2-4 with silicagel column volume ratio:1；The condensing mode adopted in step b) for concentrating under reduced pressure, pressure for -0.03～- 0.06Mpa, the temperature of concentration is 40-100 DEG C；Acetone and silica gel volume ratio are 2-4 in step c):1, acetone elution speed is 0.2-2Bv/h。

6. in a kind of Fructus Capsici as claimed in claim 5 beta-carotene preparation method, it is characterised in that in step (6), step Rapid a) neutral and alkali alcohol solution for KOH alcoholic solution, bath temperature is 40-50 DEG C, and stirring condition is 150-200r/min, saponification Reaction temperature is 50-60 DEG C, and saponification time is 2.5-3h；Acid solution is hydrochloric acid in step b), and alcohols solvent is concentration 60- 70% ethanol, bath temperature is 40-50 DEG C, and stirring condition is 150-200r/min, and the temperature of crystallize is 40 DEG C, is filtered during filtration Cloth aperture is 500 mesh；The alcohols solvent adopted in step c) for concentration 60-70% ethanol, the vacuum of drying under reduced pressure is- 0.08～-0.09MPa, drying under reduced pressure temperature is 45-50 DEG C；In step d) by the way of concentrating under reduced pressure, thickening temperature is 70- 75 DEG C, the vacuum of concentration is -0.06～-0.08Mpa.

7. in a kind of Fructus Capsici as claimed in claim 6 beta-carotene preparation method, it is characterised in that it is secondary in step (7) The ratio of product and water is 1:1, organic solvent is ethyl acetate, and by-product solution is 1 with organic solvent:1, it is spray-dried import Temperature is 185-195 DEG C, and spray drying exit temperature is 85-90 DEG C.