CN106577470B - A method of improving naked mole breeding potential - Google Patents
A method of improving naked mole breeding potential Download PDFInfo
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- CN106577470B CN106577470B CN201610910853.8A CN201610910853A CN106577470B CN 106577470 B CN106577470 B CN 106577470B CN 201610910853 A CN201610910853 A CN 201610910853A CN 106577470 B CN106577470 B CN 106577470B
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- naked mole
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- smear
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- 238000000034 method Methods 0.000 title claims abstract description 13
- 238000009395 breeding Methods 0.000 title claims abstract description 9
- 230000001488 breeding effect Effects 0.000 title claims abstract description 9
- 238000011121 vaginal smear Methods 0.000 claims abstract description 11
- 230000003542 behavioural effect Effects 0.000 claims abstract description 5
- 230000012173 estrus Effects 0.000 claims description 11
- 210000004027 cell Anatomy 0.000 claims description 6
- 210000002919 epithelial cell Anatomy 0.000 claims description 6
- 238000001514 detection method Methods 0.000 claims description 5
- 239000007788 liquid Substances 0.000 claims description 5
- 210000000436 anus Anatomy 0.000 claims description 4
- 230000001850 reproductive effect Effects 0.000 claims description 4
- 210000003708 urethra Anatomy 0.000 claims description 4
- 241000252229 Carassius auratus Species 0.000 claims description 3
- 239000013078 crystal Substances 0.000 claims description 3
- 238000002425 crystallisation Methods 0.000 claims description 3
- 230000008025 crystallization Effects 0.000 claims description 3
- 230000000994 depressogenic effect Effects 0.000 claims description 3
- 230000037213 diet Effects 0.000 claims description 3
- 235000005911 diet Nutrition 0.000 claims description 3
- 238000005286 illumination Methods 0.000 claims description 3
- 210000002510 keratinocyte Anatomy 0.000 claims description 3
- 239000007937 lozenge Substances 0.000 claims description 3
- 238000012544 monitoring process Methods 0.000 claims description 3
- 210000000056 organ Anatomy 0.000 claims description 3
- 238000012216 screening Methods 0.000 claims description 3
- 238000002834 transmittance Methods 0.000 claims description 3
- 238000004220 aggregation Methods 0.000 claims description 2
- 230000002776 aggregation Effects 0.000 claims description 2
- 241001465754 Metazoa Species 0.000 abstract description 7
- 230000013011 mating Effects 0.000 abstract description 7
- 230000007758 mating behavior Effects 0.000 abstract description 4
- 230000035935 pregnancy Effects 0.000 abstract description 3
- 241000699666 Mus <mouse, genus> Species 0.000 description 7
- 241000699670 Mus sp. Species 0.000 description 7
- 230000033458 reproduction Effects 0.000 description 7
- 230000006399 behavior Effects 0.000 description 3
- 238000010171 animal model Methods 0.000 description 2
- 238000004043 dyeing Methods 0.000 description 2
- 239000002504 physiological saline solution Substances 0.000 description 2
- 241000256844 Apis mellifera Species 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 244000144987 brood Species 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 210000003097 mucus Anatomy 0.000 description 1
- 230000016087 ovulation Effects 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000014639 sexual reproduction Effects 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/02—Breeding vertebrates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K29/00—Other apparatus for animal husbandry
- A01K29/005—Monitoring or measuring activity, e.g. detecting heat or mating
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Biophysics (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Eye Examination Apparatus (AREA)
Abstract
The present invention relates to and technical field of animal reproduction, and in particular to a method of improve naked mole breeding potential.The present invention is by detecting naked mole animal behavioral study, vaginal orifice state observation, vaginal smear, whether the naked mole of accurate judgement is in heat, and it further selects the naked mole of male in same cage source to be put into 1:1 in the naked mole rearging cage of female and mates, mating behavior observation is carried out, thus whether achieving the purpose that identify its mating.Propagation method of the invention is easy to operate, heat, mating accuracy of judgement, and naked mole Pregnancy Success rate is high.
Description
Technical field
The present invention relates to and technical field of animal reproduction, and in particular to a method of improve naked mole breeding potential.
Background technique
Naked mole is that the unique mammality that eusociality life is sought as the insects such as honeybee, ant found so far moves
Object, only only male mouse are in dominant position and can raise up seed for the naked mole of a female and 1-3 in group.Naked mole battalion is very social
Sexual reproduction mode is unfavorable for naked mole quality of heredity control, also restricts the foundation of naked mole closed colony and inbred strais population.For
Standardized laboratory animals population is established, study group attempts to break the modes of reproduction of eusociality group, naked mole battalion, establish
In the modes of reproduction at the mating of male and female 1:1 ratio (more than the monthly age, male 12 is more than the monthly age for female mice 10), this modes of reproduction facilitates
Naked mole heredity and microbial quality are controlled, naked mole is made to become standardized experimental animal.Wherein, can naked mole normal numerous
It grows, depends primarily on and accurately find its heat and breed in time.
This laboratory early period is bred using primary community's modes of reproduction, and there are about tens naked mole groups for each group
At wherein only a female mice can be bred, other female mices are irreproducible, and this modes of reproduction reproductive efficiency is very low, cause naked
Mole fertility is very low.And when being cultivated according to mouse closed colony modes of reproduction early period, find not brood other male and female mouse
It can mutually be baited when mating, having more than 1/3 pairing individual can not normally breed.For this purpose, we are by exploring naked mole hair
Feelings phase behavior expression and feature mate time and the judgment method that mates etc., establish the higher naked mole heat of breeding potential and friendship
With judgment technology.
Summary of the invention
It is an object of the invention to take the deficiencies in the prior art, a kind of method for improving naked mole breeding potential is provided.
Main technical schemes of the invention are:
The purpose of the present invention is to provide a kind of methods for improving naked mole breeding potential, comprising:
1) naked mole behaviouristics primary dcreening operation: filter out in 1-3 days by a definite date time occur apathetic depressed, diet reduce,
The abnormal irritated naked mole of female；
2) naked mole vaginal orifice state secondary screening: between the anus and urethra outside of the naked mole of female filtered out to step 1)
Pale red whippletree shape gap is detected, and filters out cross bar color burn, gap is opened, visible obvious redness, forms the 2 of protrusion
Valve, and have the naked mole of female of transparency liquid adhesion；
3) vaginal smear detects: the naked mole of female of step 2) is carried out whether vaginal smear detection determines the naked mole of female
It is in heat；
4) male and female mouse mates: selection individual quite and with the naked mole of male in cage source be put into it is above-mentioned be in heat it is female
Property naked mole rearging cage in 1:1 mate, if there are mutual sniffs for naked mole, said with the phallic behavior of the head portion of going friction other side
The bright naked mole of female is in heat and can mate, and estrus animal, which puts together, to be easy to fight, bait；
5) male and female mouse mates: closing illumination, observes naked mole by monitoring system and mate behavioral activity, if observing male and female
Property naked mole reproductive organs contact last for several seconds clock more than, then mate success.
Further, the judgment criteria of the detection of vaginal smear described in step 3) is as follows:
A) proestrum: smear is cloud crystallization, flat epithelial cell；
B) oestrus: smear is typical fernlike crystal, and master is upright and thick, superficial cell aggregation；
C) the heat later period: smear leucocyte is in the majority, a small amount of superficial cell, and branch form is relatively fuzzy, and branch is few and dilute
Dredge into goldfish grass-like；
D) dioestrus: smear keratinocyte is constantly reduced, and leucocyte is increasing, and is converted into based on leucocyte, even
See flat epithelial cell, ellipse or lozenge, light transmittance are big.
Further, monitoring system described in step 5) is infrared monitor control device and video camera tracking system.
The present invention is by detecting naked mole animal behavioral study, vaginal orifice state observation, vaginal smear, the naked mole of accurate judgement
Whether it is in heat, and further selects the naked mole of male in same cage source to be put into 1:1 in the naked mole rearging cage of female and mate,
Mating behavior observation is carried out, thus whether achieving the purpose that identify its mating.
Propagation method of the invention is easy to operate, heat, mating accuracy of judgement, and naked mole Pregnancy Success rate is high.
Detailed description of the invention
Fig. 1 is naked mole oestrus and estrus vaginal orifice state vs；It wherein, a) is non-heat female mice；It b) is heat
Female mice；
Fig. 2 is naked mole oestrous cycle vaginal smear judgment criteria；It wherein, a) is proestrum；It b) is oestrus；C) it is
The heat later period；It d) is dioestrus.
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation description, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.It is based on
Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts every other
Embodiment shall fall within the protection scope of the present invention.
Naked mole used in experimental implementation is that Second Military Medical University, PLA's experiment is dynamic in following embodiment
The naked mole population of object center raising in 2014 to 2016, proceeds as follows:
Embodiment 1
1) naked mole behaviouristics primary dcreening operation:
Filter out the naked mole of female for occurring that apathetic depressed, diet is reduced, exception is irritated in 1-3 days by a definite date time
Mouse；In estrus, the naked mole animation of female, which has no feature, to follow；
2) naked mole vaginal orifice state secondary screening
Pale red whippletree shape gap between the anus and urethra outside of the naked mole of female filtered out to step 1) carries out
Detection, there is pale red whippletree shape gap (Fig. 1 a), the female mice in the oestrus cross between the female mice anus and urethra outside of non-heat
Bar color burn, gap are opened, it is seen that and it is apparent red and swollen, 2 valves of protrusion are formed, and have transparency liquid adhesion (Fig. 1 b)；
3) vaginal smear detects
HE dyeing is carried out again after the naked mole individual of female of step 2) is carried out Ovulation prediction smear, whether determines naked mole
It is in heat；Specific steps are as follows: by naked mole Baoding, overturn tail and exposed introitus, then with a small amount of physiological saline of suction
Introitus is instilled, squeezing naked mole waist flows out mucus with physiological saline, sucks in dropper, drips on glass slide, and smear is certainly
It is so dry, Rui Shi-Jim Sa liquid dyeing is added dropwise, the phosphate buffer of equivalent is added dropwise after 1min, jiggles slide, makes two liquid
It is uniformly mixed, continues 10-15min flowing water and wash away dye liquor, microscopically observation cellular morphology after smear air-dries；
Naked mole oestrous cycle vaginal smear judgment criteria is as follows:
1. proestrum: smear is cloud crystallization, flat epithelial cell (Fig. 2 a)；
2. oestrus: smear is typical fernlike crystal, and master is upright and thick, and superficial cell assembles (Fig. 2 b)；
3. the heat later period: smear leucocyte is in the majority, a small amount of superficial cell, and branch form is relatively fuzzy, and branch is few and dilute
Dredge into goldfish grass-like (Fig. 2 c)；
4. dioestrus: smear keratinocyte is constantly reduced, and leucocyte is increasing, and is converted into based on leucocyte, even
See flat epithelial cell, ellipse or lozenge, light transmittance is big (Fig. 2 d).
4) male and female mouse mates
Different cage domesticated animals, which put together, to be easy to fight, bait, therefore should be selected individual quite and with cage source
The naked mole of male is put into 1:1 in the naked mole rearging cage of female and mates, and there are mutual sniffs for such as naked mole, goes friction other side with head
Phallic behavior both illustrate to mate；
5) mating behavior is observed
Illumination is closed to observe naked mole using infrared monitor control device and video camera tracking system and mate behavioral activity,
If it is observed that the female naked mole reproductive organs of male contacts clock for 10 seconds or more and proves to mate successfully.
By being observed altogether it has been observed that the naked mole raised uses the above method to determine female naked mole oestrus
130 female naked moles are in heat.What the naked mole of heat had mating behavior after mating has 112, this 112 all pregnancies,
Accuracy is 100%.
The preferred embodiment of the present invention has been described in detail above, but the invention be not limited to it is described
Embodiment, those skilled in the art can also make various equivalent on the premise of not violating the inventive spirit of the present invention
Variation or replacement, these equivalent variation or replacement are all included in the scope defined by the claims of the present application.
Claims (2)
1. a kind of method for improving naked mole breeding potential, comprising:
1) it naked mole behaviouristics primary dcreening operation: filters out in 1-3 days by a definite date time and apathetic depressed, diet reduction, exception occurs
The irritated naked mole of female；
2) naked mole vaginal orifice state secondary screening: light red between the anus and urethra outside of the naked mole of female filtered out to step 1)
Color whippletree shape gap is detected, and cross bar color burn, gap opening, visible obvious 2 valves that are red and swollen, forming protrusion are filtered out,
And there is the naked mole of female of transparency liquid adhesion；
3) vaginal smear detects: the naked mole of female of step 2) being carried out vaginal smear detection and determines whether the naked mole of female is in
Oestrus；
4) male and female mouse mates: it is naked that selection individual quite and with the naked mole of male in cage source is put into the above-mentioned female being in heat
1:1 is mated in mole rearging cage, if there are mutual sniffs for naked mole, is illustrated with the phallic behavior of the head portion of going friction other side female
The naked mole of property is in heat and can mate；
5) male and female mouse mates: closing illumination, observes naked mole by monitoring system and mate behavioral activity, female male is naked if observing
Mole reproductive organs contacts clock for 10 seconds or more, then mate success；
The judgment criteria of the detection of vaginal smear described in step 3) is as follows:
A) proestrum: smear is cloud crystallization, flat epithelial cell；
B) oestrus: smear is typical fernlike crystal, and master is upright and thick, superficial cell aggregation；
C) the heat later period: smear leucocyte is in the majority, a small amount of superficial cell, and branch form is relatively fuzzy,
Branch is few and sparse at goldfish grass-like；
D) dioestrus: smear keratinocyte is constantly reduced, and leucocyte is increasing, and is converted into based on leucocyte, accidental flat
Flat epithelial cell, ellipse or lozenge, light transmittance are big.
2. a kind of method for improving naked mole breeding potential according to claim 1, it is characterised in that: supervised described in step 5)
Control system is infrared monitor control device and video camera tracking system.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610910853.8A CN106577470B (en) | 2016-10-19 | 2016-10-19 | A method of improving naked mole breeding potential |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610910853.8A CN106577470B (en) | 2016-10-19 | 2016-10-19 | A method of improving naked mole breeding potential |
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| CN106577470A CN106577470A (en) | 2017-04-26 |
| CN106577470B true CN106577470B (en) | 2019-07-02 |
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| CN107771705A (en) * | 2017-09-08 | 2018-03-09 | 东北林业大学 | A kind of fox ovulation period monitors new method |
| US12057374B2 (en) | 2019-01-23 | 2024-08-06 | Qorvo Us, Inc. | RF devices with enhanced performance and methods of forming the same |
| CN110514849A (en) * | 2019-08-24 | 2019-11-29 | 牡丹江师范学院 | A kind of super early pregnancy detection method of Northeast Female tiger |
| CN110447599B (en) * | 2019-08-30 | 2021-09-24 | 广西医科大学 | Comprehensive evaluation method for fertility of male mice |
| CN113229172A (en) * | 2021-05-07 | 2021-08-10 | 广州医药研究总院有限公司 | Method for releasing natural mating of dogs at proper time |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1150886A (en) * | 1996-09-24 | 1997-06-04 | 梁建友 | Method for artificial raising, training and breeding bamboo rat |
| CN104054632A (en) * | 2014-07-04 | 2014-09-24 | 神农架圣旺生态养殖有限公司 | Bamboo rat breeding method |
| WO2015013575A2 (en) * | 2013-07-25 | 2015-01-29 | Ingenious Targeting Laboratory, Inc. | Knockout mice derived from site specific recombinase |
| CN104396862A (en) * | 2014-10-17 | 2015-03-11 | 何兵 | Rhizomyidae traditional Chinese medicine feed reproduction-promoting method |
-
2016
- 2016-10-19 CN CN201610910853.8A patent/CN106577470B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1150886A (en) * | 1996-09-24 | 1997-06-04 | 梁建友 | Method for artificial raising, training and breeding bamboo rat |
| WO2015013575A2 (en) * | 2013-07-25 | 2015-01-29 | Ingenious Targeting Laboratory, Inc. | Knockout mice derived from site specific recombinase |
| CN104054632A (en) * | 2014-07-04 | 2014-09-24 | 神农架圣旺生态养殖有限公司 | Bamboo rat breeding method |
| CN104396862A (en) * | 2014-10-17 | 2015-03-11 | 何兵 | Rhizomyidae traditional Chinese medicine feed reproduction-promoting method |
Non-Patent Citations (1)
| Title |
|---|
| 发情雌鼠鉴定对交配成功率的影响;李雨珊等;《中国现代医药杂志》;20111130;第13卷(第11期);第5-7页 |
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