CN106568948A - Immunochromatographic detection device - Google Patents
Immunochromatographic detection device Download PDFInfo
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- CN106568948A CN106568948A CN201610886178.XA CN201610886178A CN106568948A CN 106568948 A CN106568948 A CN 106568948A CN 201610886178 A CN201610886178 A CN 201610886178A CN 106568948 A CN106568948 A CN 106568948A
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- 238000012360 testing method Methods 0.000 claims abstract description 58
- 239000000463 material Substances 0.000 claims abstract description 38
- 230000005284 excitation Effects 0.000 claims abstract description 20
- 238000002834 transmittance Methods 0.000 claims abstract description 20
- 238000003317 immunochromatography Methods 0.000 claims description 10
- 239000004425 Makrolon Substances 0.000 claims description 4
- 229920006217 cellulose acetate butyrate Polymers 0.000 claims description 4
- 229920000515 polycarbonate Polymers 0.000 claims description 4
- 229920000728 polyester Polymers 0.000 claims description 4
- -1 polyethylene terephthalate Polymers 0.000 claims description 4
- NIXOWILDQLNWCW-UHFFFAOYSA-N acrylic acid group Chemical group C(C=C)(=O)O NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 claims description 3
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- 238000004587 chromatography analysis Methods 0.000 description 15
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- 102000036639 antigens Human genes 0.000 description 7
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 7
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- 238000010382 chemical cross-linking Methods 0.000 description 3
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54366—Apparatus specially adapted for solid-phase testing
- G01N33/54386—Analytical elements
- G01N33/54387—Immunochromatographic test strips
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- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Analytical Chemistry (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Food Science & Technology (AREA)
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- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
The invention relates to the technical field of immunoassay, and particularly discloses an immunochromatographic detection device including a test strip and a detection device; the test strip includes a multilayer porous membrane and a support sheet; the support sheet is a material having less than or equal to 10% of light transmittance at the wavelength of less than 450 nm and having more than or equal to 90% of light transmittance at the wavelength of more than or equal to 500 nm; the detection device includes an excitation light source, a detection zone for placing the test strip and a light detector; the excitation light source and the light detector are respectively located at both sides of the detection zone. With adopting of a transmission method, the light detector can capture fluorescent signals of the surface and the interior of the multilayer porous membrane, and the detection result is more accurate.
Description
Technical field
The present invention relates to technical field of immunoassay, in particular it relates to a kind of immuno-chromatography detection device.
Background technology
Immunochromatographic method (immunochromatography) is a kind of quick diagnosis technology of external rise in recent years,
Its principle is a certain zone that special antibody is first fixed on nitrocellulose filter, when celluloid one end of the drying is soaked
After entering sample (urine or serum), due to capillarity, sample will be moved forward along the film, and when being moved to antibody is fixed with
Region when, corresponding antigen specifically binds with the antibody in sample, if with immune colloid gold or Immunoperoxidase Staining
The region can be made to show certain color provide the qualitative or half-quantitative detection data to sample, so as to realize specificity
Immunologic diagnosises.Compared with routine diagnostic method, the technical Analysis speed is fast, and whole detection process only need 5～30 minutes.Operation letter
Just, it is not necessary to other any instruments, without the need for professional, for real-time on-site detection condition is provided.Detection sample species is more, can
For the micro solution of the preparations such as the blood in medical treatment, health, food safety, environment measuring, saliva, food, water quality, soil.
Immuno-chromatographic test paper strip is the atopic and chromatographic technique for combining immune marker and corresponding antigen (antibody)
And made by.Immune chromatograph testing strip is generally made up of sample application zone, reaction zone and the part of suction zones three.Contain immune mark in sample application zone
Note composition granule, generally by glass fibre by immune colloid gold granular absorption in the area；Reaction zone then sprays two response lines, one
For detection line, one is nature controlling line.Detection line is to detect the envelope antigen of (antigen) material and immune labeled composition granule herein
The reactivity of (antigen that antibody or antibody are carried)；Nature controlling line is then to detect the coating protein matter on immune labeled composition granule
Activity and degree, after immune marker is by sample application zone release, reacted area partly proceeds to adsorption zone, chromatography is completed, so as to reach
To the purpose of quick detection.
Newest immunochromatographic method is coupled to specific antibody using specific effect between chemical crosslinking or biomolecule
The surface of fluorescence molecule or fluorescent grain, respectively obtains the specific antibody of fluorescent dye modification and the Quality Control of fluorescent dye modification
Molecule.When fluorescent dye surface has active group, it directly can be reacted with specific antibody, be not required to chemical crosslinking
Agent；Conversely, then needing mutually to be coupled fluorescent dye with specific antibody or Quality Control molecule by chemical cross-linking agent.Wherein, chemistry is handed over
Connection agent includes 1- ethyl -3- (3- DimethylAminopropyls) carbodiimides (EDC), N-hydroxy-succinamide (NHS) and penta 2
Aldehyde etc..
In order to improve the discrimination of signal and background, the fluorescent wavelength ranges of fluorescent dye should be 550～800nm.Because
Under ultra-vioket radiation, the autofluorescence intensity of chromatographic film, base plate and buckle is understood much stronger than more than 550nm in below 550nm, so as to
Certain impact can be produced to the detection of low concentration albumen, therefore preferred emission wavelength is more than the fluorescent dye of 550nm；Additionally, layer
Analysis film, base plate and buckle are typically extremely weak near infrared region (600～800nm) fluorescence intensity, it is therefore preferable that wave-length coverage is
The fluorescent dye of 550～800nm is further improving sensitivity.Fluorescent dye includes organic fluorescent dye and rare earth element fluorescence
The fluorescein of dyestuff, such as AlexaFluro series.
The immunofluorescence chromatographic apparatus for adopting at present detect the fluorescence signal on perforated membrane, fluorescence detector using bounce technique
What is captured is the specific antibody of porous film surface fluorescent dye modification, and can't detect the fluorescence signal inside perforated membrane,
Detection sensitivity is caused to decline.
The content of the invention
Above-mentioned deficiency in order to overcome prior art of the invention, there is provided a kind of immuno-chromatography detection device, the device is created
Property adopt transmission beam method, photodetector can catch the fluorescence signal of multilayer porous film surface and inside, make testing result more accurate
Really.
To achieve these goals, the present invention is achieved by the following technical programs：
A kind of immuno-chromatography detection device, is made up of test strips and detector, the test strips include multilayer porous film and
Support chip, support chip is have less than 10% light transmittance less than 450nm wavelength, has more than 90% more than 500nm wavelength
Light transmittance material；Detector inside cavity includes excitation source, places the detection zone and photodetector of test strips, described to swash
Luminous source and photodetector are respectively positioned at the both sides of detection zone.
The building block of detector of the present invention is identical with the part composition of traditional immunochromatography detector, uniquely not
It is that the excitation source and photodetector in detector of the present invention is respectively positioned at the both sides of detection zone with part；And traditional inspection
The excitation source surveyed in device and photodetector are located at the same side of detection zone.This difference is that traditional detector is to utilize
Fluorescence signal on bounce technique detection multilayer porous film, and the present invention is that the fluorescence letter on multilayer porous film is detected by transmission beam method
Number.In order to coordinate the realization of transmission beam method, the present invention to improve the material of the support chip in traditional test strip, used
There is less than 10% light transmittance less than 450nm wavelength, there is the material of more than 90% light transmittance more than 500nm wavelength.This
Planting material can allow most of visible light-transmissive.So excitation source can penetrate the surface and inside of multilayer porous film,
Photodetector can catch the fluorescence signal of multilayer porous film surface and inside, make testing result more accurate.
According to support chip for the transmission of light sources with different wavelengths is required, support chip can be colorless or colored, plastics
The holder of material can mix dyestuff and pigment with the transmission for attracting the photon of certain wavelength to meet for light sources with different wavelengths
Require.Preferably, the support chip is polyester, acrylic, Merlon, polyacrylate, glycol-modified poly- to benzene two
Formic acid glycol ester or cellulose acetate-butyrate material.
It is highly preferred that the support chip is makrolon material.Merlon be in optical resin material refraction index compared with
High kind, its light transmittance is suitable with unorganic glass, and refractive index reaches 1.59, density for unorganic glass half, can freely into
Type is arbitrary shape, with good biocompatibility.
Compared with prior art, the present invention has the advantages that：
Traditional immunofluorescence chromatography method is that a certain amount of sample is added drop-wise in sample pad, by slab effect forward
Mobile, the labelled reagent being dissolved on pad reacts with sample, when conjugate is moved to Detection of antigen band, determinand and knot
The reaction that compound occurs specificity is trapped, and conjugate is enriched with detection line, and conjugate content contains with the determinand in sample
Amount is directly proportional.The fluorescence intensity produced under excitation source is directly proportional to the conjugate content in test strips, when light source is irradiated to
When the detection zone and quality control region of test strips, the fluorescent material of attachment, emission light gathering is excited simultaneously to be converted into the signal of telecommunication, the signal of telecommunication
Strong and weak related to fluorescence molecule quantity, detector calculates the content of determinand in sample.Transmission beam method is than traditional method for reflection
Advantage is that sensitivity is high.Because traditional method for reflection simply gathers the antibody signal of membrane surface, and transmission beam method connects in film
The antibody response signal in portion can be gathered, so signal can be higher.
Description of the drawings
Fig. 1 is the schematic perspective view of detector.
Fig. 2 is detector inside cavity structural representation.
Fig. 3 is the result schematic diagram of test card slot.
Fig. 4 is and examination in hospital data dependence comparative result.
Specific embodiment
The present invention is made with reference to Figure of description and specific embodiment further being elaborated, the embodiment
It is served only for explaining the present invention, is not intended to limit the scope of the present invention.Test method used in following embodiments is such as without spy
Different explanation, is conventional method；Material, reagent for being used etc., are the reagent for commercially obtaining if no special instructions
And material.
Embodiment 1
A kind of fluorescence immune chromatography detection means, including test strips and detection means, the test strips include multi-layer porous
Film and support chip, multilayer porous film is respectively sample pad, pad, nitrocellulose filter and absorbent paper, is fixed with pad
Fluorescent labeling reagent, nitrocellulose filter is provided with detection line and control line, sample pad, pad, nitrocellulose filter and water suction
Paper is sequentially mutually pasted onto on support chip overlap joint, so as to obtain test strips.Support chip is makrolon material in the present embodiment.
Makrolon material less than 400nm wavelength have less than 10% light transmittance, more than 500nm wavelength have more than 90% it is saturating
The material of light rate, can guarantee that ultraviolet light, it is seen that light and ultrared transmission.
The schematic perspective view of detector is shown in Fig. 1, and detector inside cavity structural representation is shown in Fig. 2.Detector includes housing
With as the excitation source in housing, place test strips test card slot, photodetector, microcontroller, the excitation source and
Photodetector is connected respectively by detection circuit positioned at the both sides of test card slot, excitation source and photodetector with microcontroller.
The surface of shell of detector is provided with display screen and a draw-in groove opening, can be test card slot from detector housing by opening
Release, the extraction mode of test card slot can be drawing and pulling type or Pop-up, and test card slot is the three-back-shaped card of middle opening
Groove (see Fig. 3), test strips is put in test card slot, and two of test strips is tried after rotary pressure plate or other devices are fixed
Detection line and control line on paper slip just falls in the central closing of square shape draw-in groove, and such excitation source is by test strips
Transmitted light can be detected without any stop by photodetector.
The using method of fluorescence immune chromatography detection means is described in the present embodiment, and a certain amount of sample to be tested is added drop-wise to
In sample pad, moved forward by slab effect, the labelled reagent being dissolved on pad reacts with sample, when conjugate movement
During to Detection of antigen line, the reaction that determinand occurs specificity with conjugate is trapped, and conjugate is enriched with detection line, with reference to
Thing content is directly proportional to the determinand content in sample.Conjugate in the fluorescence intensity produced under excitation source and test strips
Content is directly proportional, and when microprocessor controls excitation source is irradiated to the detection line and control line of test strips, excites the fluorescence of attachment
Material, photodetector is collected by the transmitted light in test strips, and is converted into the signal of telecommunication, the power and fluorescence molecule number of the signal of telecommunication
Amount is related, and the signal of telecommunication sends microcontroller to, and microcontroller receives current signal, carries out conversion process analysis, and test sample is treated in calculating
The content of determinand in product, microcontroller shows the result of Treatment Analysis by the display screen of detector surface of shell.
Embodiment 2
A kind of fluorescence immune chromatography detection means, including test strips and detection means, the test strips include multi-layer porous
Film and support chip, multilayer porous film is respectively sample pad, pad, nitrocellulose filter and absorbent paper, is fixed with pad
Fluorescent labeling reagent, nitrocellulose filter is provided with detection line and control line, sample pad, pad, nitrocellulose filter and water suction
Paper is sequentially mutually pasted onto on support chip overlap joint, so as to obtain test strips.Support chip is acrylic material in the present embodiment.It is sub-
Gram force material 400nm wavelength has less than 10% light transmittance, the material more than 500nm wavelength with more than 90% light transmittance
Material, can guarantee that ultraviolet light, it is seen that light and ultrared transmission.
Detector is constituted and fluorescent chromatography detector phase commonly used in the prior art with the component of embodiment 1 or detector
Together, unique except for the difference that excitation source and photodetector are respectively positioned at the both sides of detection zone.
Embodiment 3
A kind of fluorescence immune chromatography detection means, including test strips and detection means, the test strips include multi-layer porous
Film and support chip, multilayer porous film is respectively sample pad, pad, nitrocellulose filter and absorbent paper, is fixed with pad
Fluorescent labeling reagent, nitrocellulose filter is provided with detection line and control line, sample pad, pad, nitrocellulose filter and water suction
Paper is sequentially mutually pasted onto on support chip overlap joint, so as to obtain test strips.Support chip is polyester material in the present embodiment.Polyester
Material has less than 10% light transmittance less than 400nm wavelength, the material more than 500nm wavelength with more than 90% light transmittance
Material, can guarantee that ultraviolet light, it is seen that light and ultrared transmission.
Detector is constituted and fluorescent chromatography detector phase commonly used in the prior art with the component of embodiment 1 or detector
Together, unique except for the difference that excitation source and photodetector are respectively positioned at the both sides of detection zone.
Embodiment 4
A kind of fluorescence immune chromatography detection means, including test strips and detection means, the test strips include multi-layer porous
Film and support chip, multilayer porous film is respectively sample pad, pad, nitrocellulose filter and absorbent paper, is fixed with pad
Fluorescent labeling reagent, nitrocellulose filter is provided with detection line and control line, sample pad, pad, nitrocellulose filter and water suction
Paper is sequentially mutually pasted onto on support chip overlap joint, so as to obtain test strips.Support chip is glycol-modified in the present embodiment
Pet material.Glycol-modified pet material is less than 400nm wavelength
With less than 10% light transmittance, there is the material of more than 90% light transmittance more than 500nm wavelength, can guarantee that ultraviolet light, can
See light and ultrared transmission.
Detector is constituted and fluorescent chromatography detector phase commonly used in the prior art with the component of embodiment 1 or detector
Together, unique except for the difference that excitation source and photodetector are respectively positioned at the both sides of detection zone.
Embodiment 5
A kind of fluorescence immune chromatography detection means, including test strips and detection means, the test strips include multi-layer porous
Film and support chip, multilayer porous film is respectively sample pad, pad, nitrocellulose filter and absorbent paper, is fixed with pad
Fluorescent labeling reagent, nitrocellulose filter is provided with detection line and control line, sample pad, pad, nitrocellulose filter and water suction
Paper is sequentially mutually pasted onto on support chip overlap joint, so as to obtain test strips.Support chip is cellulose acetate butyrate in the present embodiment
Cellulosic material.Cellulose acetate-butyrate material has less than 10% light transmittance less than 400nm wavelength, has more than 500nm wavelength
There is the material of more than 90% light transmittance, can guarantee that ultraviolet light, it is seen that light and ultrared transmission.
Detector is constituted and fluorescent chromatography detector phase commonly used in the prior art with the component of embodiment 1 or detector
Together, unique except for the difference that excitation source and photodetector are respectively positioned at the both sides of detection zone.
Embodiment 6
A kind of fluorescence immune chromatography detection means, including test strips and detection means, the test strips include multi-layer porous
Film and support chip, multilayer porous film is respectively sample pad, pad, nitrocellulose filter and absorbent paper, is fixed with pad
Fluorescent labeling reagent, nitrocellulose filter is provided with detection line and control line, sample pad, pad, nitrocellulose filter and water suction
Paper is sequentially mutually pasted onto on support chip overlap joint, so as to obtain test strips.Support chip is polyacrylate material in the present embodiment
Material.Polyacrylate material less than 450nm wavelength have less than 10% light transmittance, more than 500nm wavelength have 90% with
On light transmittance material, can guarantee that ultraviolet light, it is seen that light and ultrared transmission.
Detector is constituted and fluorescent chromatography detector phase commonly used in the prior art with the component of embodiment 1 or detector
Together, unique except for the difference that excitation source and photodetector are respectively positioned at the both sides of detection zone.
Application examples 1
It is used for the Immunofluorescence test of troponin using the immuno-chromatography detection device described in embodiment 1, step is as follows：
1st, test strip and whole blood/serum/plasma specimen are recovered to room temperature (20～30 DEG C) using front, takes out reaction
Buffer is simultaneously placed 20 minutes and makes it restore to room temperature.
2nd, test strips are placed on clean flat table top, drawn with suction pipe instill after finger tip blood or venous blood 1 drip whole blood/
Serum/plasma (about 25ml) is in sample pad.
3rd, test strips are placed in the test card slot of detector at 15 minutes or so, detector read test result.
4th, result judgement is carried out according to detector data.
To be contrasted with the correlation data of hospital using the testing result of immuno-chromatography detection device of the present invention,
The results are shown in Table 1 and Fig. 4.As seen from Figure 4, illustrate that the result detected using instrument of the present invention is had with hospital testing result
Good dependency, the accuracy rate of instrument of the present invention is higher.
Table 1 is the result that troponin is detected using immuno-chromatography detection device of the present invention
Claims (3)
1. a kind of immuno-chromatography detection device, is made up of test strips and detector, and the test strips include multilayer porous film and prop up
Blade；Detector inside cavity includes excitation source, places the detection zone and photodetector of test strips, it is characterised in that described
The support chip of test strips is have less than 10% light transmittance less than 450nm wavelength, has more than 90% more than 500nm wavelength
The material of light transmittance；The excitation source and photodetector are respectively positioned at the both sides of detection zone.
2. device according to claim 1, it is characterised in that the support chip is polyester, acrylic, Merlon, poly-
Acrylate, glycol-modified polyethylene terephthalate or cellulose acetate-butyrate material.
3. device according to claim 1, it is characterised in that the support chip is makrolon material.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610886178.XA CN106568948A (en) | 2016-10-10 | 2016-10-10 | Immunochromatographic detection device |
| PCT/CN2017/098112 WO2018068582A1 (en) | 2016-10-10 | 2017-08-18 | Immunochromatography detection apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610886178.XA CN106568948A (en) | 2016-10-10 | 2016-10-10 | Immunochromatographic detection device |
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| CN106568948A true CN106568948A (en) | 2017-04-19 |
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| CN201610886178.XA Pending CN106568948A (en) | 2016-10-10 | 2016-10-10 | Immunochromatographic detection device |
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| WO (1) | WO2018068582A1 (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107505459A (en) * | 2017-07-03 | 2017-12-22 | 广州瑞博奥生物科技有限公司 | Quantitatively detect people H FABP time-resolved fluoroimmunoassay chromatograph test strip, kit and preparation method thereof |
| CN107655889A (en) * | 2017-09-22 | 2018-02-02 | 暨南大学 | Colorimetric chromatograph test strip based on smart mobile phone reads detection means and application |
| WO2018068582A1 (en) * | 2016-10-10 | 2018-04-19 | 广州瑞博奥生物科技有限公司 | Immunochromatography detection apparatus |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
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Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1134750A (en) * | 1993-11-12 | 1996-10-30 | 尤尼帕思有限公司 | Reading devices for teststrips |
| US20040191119A1 (en) * | 2003-03-24 | 2004-09-30 | Zanzucchi Peter J. | Analyte concentration detection devices and methods |
| US20050153277A1 (en) * | 2004-01-13 | 2005-07-14 | Kai-Tsung Teng | Immunochemical assay device |
| US20060019265A1 (en) * | 2004-04-30 | 2006-01-26 | Kimberly-Clark Worldwide, Inc. | Transmission-based luminescent detection systems |
| CN1854738A (en) * | 2005-04-20 | 2006-11-01 | 北京怡成生物电子技术有限公司 | Microdose urine protein testing chip and its tester |
| CN101115994A (en) * | 2005-02-04 | 2008-01-30 | 斯塔戈诊断公司 | Immunochromatographic method for the quantitative measurement of analytes in a liquid sample |
| CN202002929U (en) * | 2010-11-18 | 2011-10-05 | 苏州生物医学工程技术研究所 | Multilayer dry membrane sheet for biochemical analysis |
| CN103308489A (en) * | 2012-01-02 | 2013-09-18 | 何爱民 | Laterally-flowing immunoassay method using time resolution up-converting phosphor technology |
| CN104160277A (en) * | 2012-01-11 | 2014-11-19 | 嘉泉大学校产学协力团 | Blood glucose measurement unit, blood glucose measurement system comprising same, and blood glucose measurement method |
| CN105992952A (en) * | 2014-02-13 | 2016-10-05 | 莫勒库拉尔维森有限公司 | Assay device |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20130273563A1 (en) * | 2012-04-17 | 2013-10-17 | Joel R. L. Ehrenkranz | Device for performing an enzyme-based diagnostic test and methods for use thereof |
| US9726605B2 (en) * | 2013-03-14 | 2017-08-08 | Furukawa Electric Co., Ltd. | Fluorescence immuno-chromatography, kit and test strip for the same |
| CN203759019U (en) * | 2014-01-28 | 2014-08-06 | 上海道原生物科技有限公司 | Immunochromatographic detection device |
| CN105242039A (en) * | 2015-10-29 | 2016-01-13 | 詹爱军 | Fluorescent immunochromatography quantitative analyzer and method |
| CN106568948A (en) * | 2016-10-10 | 2017-04-19 | 广州瑞博奥生物科技有限公司 | Immunochromatographic detection device |
-
2016
- 2016-10-10 CN CN201610886178.XA patent/CN106568948A/en active Pending
-
2017
- 2017-08-18 WO PCT/CN2017/098112 patent/WO2018068582A1/en active Application Filing
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1134750A (en) * | 1993-11-12 | 1996-10-30 | 尤尼帕思有限公司 | Reading devices for teststrips |
| US20040191119A1 (en) * | 2003-03-24 | 2004-09-30 | Zanzucchi Peter J. | Analyte concentration detection devices and methods |
| US20050153277A1 (en) * | 2004-01-13 | 2005-07-14 | Kai-Tsung Teng | Immunochemical assay device |
| US20060019265A1 (en) * | 2004-04-30 | 2006-01-26 | Kimberly-Clark Worldwide, Inc. | Transmission-based luminescent detection systems |
| CN101115994A (en) * | 2005-02-04 | 2008-01-30 | 斯塔戈诊断公司 | Immunochromatographic method for the quantitative measurement of analytes in a liquid sample |
| CN1854738A (en) * | 2005-04-20 | 2006-11-01 | 北京怡成生物电子技术有限公司 | Microdose urine protein testing chip and its tester |
| CN202002929U (en) * | 2010-11-18 | 2011-10-05 | 苏州生物医学工程技术研究所 | Multilayer dry membrane sheet for biochemical analysis |
| CN103308489A (en) * | 2012-01-02 | 2013-09-18 | 何爱民 | Laterally-flowing immunoassay method using time resolution up-converting phosphor technology |
| CN104160277A (en) * | 2012-01-11 | 2014-11-19 | 嘉泉大学校产学协力团 | Blood glucose measurement unit, blood glucose measurement system comprising same, and blood glucose measurement method |
| CN105992952A (en) * | 2014-02-13 | 2016-10-05 | 莫勒库拉尔维森有限公司 | Assay device |
Non-Patent Citations (2)
| Title |
|---|
| 《化工百科全书》编辑委员会: "《化工百科全书》", 30 June 1995, 化学工业出版社 * |
| 唐磊等: "《轻量化材料技术》", 31 January 2014, 国防工业出版社 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2018068582A1 (en) * | 2016-10-10 | 2018-04-19 | 广州瑞博奥生物科技有限公司 | Immunochromatography detection apparatus |
| CN107505459A (en) * | 2017-07-03 | 2017-12-22 | 广州瑞博奥生物科技有限公司 | Quantitatively detect people H FABP time-resolved fluoroimmunoassay chromatograph test strip, kit and preparation method thereof |
| CN107505459B (en) * | 2017-07-03 | 2019-12-24 | 广州瑞博奥生物科技有限公司 | Time-resolved fluorescence immunochromatographic test strip and kit for quantitatively detecting human H-FABP and preparation method thereof |
| CN107655889A (en) * | 2017-09-22 | 2018-02-02 | 暨南大学 | Colorimetric chromatograph test strip based on smart mobile phone reads detection means and application |
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