CN106544280B - A kind of resistance meat duck yeast for animal feeds bacterial strain and application - Google Patents
A kind of resistance meat duck yeast for animal feeds bacterial strain and application Download PDFInfo
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Abstract
The present invention provides a kind of resistance meat duck yeast for animal feeds bacterial strain and application, after the steps include: that A. takes the gastral content of meat duck to be diluted, plate streaking is carried out, selects and is further separated with the conventional consistent bacterium colony of S. cervisiae form.B. the S. cervisiae of acquisition heat resistance, acid resistance, resistance to nutrition starvation and bile tolerance is carried out to screen.C. isolated saccharomycete is subjected to Physiology and biochemistry identification and 26s rDNA Molecular Identification.For the Pseudomonas in saccharomyces cerevisiae, prominent characteristic is the ability of acidproof, bile tolerance with higher, heat-resisting and resistance to nutrition starvation.Being added in meat duck mixed feed with the bacterial strain is that mainly active probiotics can remarkably promote the production performance of meat duck, the use of alternative antibiotic.The saccharomyces cerevisiae (Saccharomyces cerevisiae) for WHY-7 in China typical culture collection center preservation, number is CCTCC NO:M 2016550.
Description
Technical field
The present invention relates to microorganisms technical fields, and in particular to a kind of resistance meat duck yeast for animal feeds bacterial strain, while also relating to
And a kind of purposes of resistance meat duck yeast for animal feeds bacterium.
Background technique
Abuse of antibiotics causes serious threat to the development of poultry industry, causes day to the health of animals and humans
The harm of benefit accumulation.So people need to find and develop the nontoxic spontaneous growth promotor having no adverse reaction, then probiotics
Feed addictive comes into being.Probiotics can improve the productivity of animal by adjusting the microecological balance of animal gastrointestinal tract
Can, improve the general level of the health of animal.As nontoxic, noresidue and without the feed addictive of drug resistance, probiotics has become antibiosis
One of plain effective substitute.
Saccharomycete (Saccharomyces cerevisiae) is allowed for by many countries and regions as probiotics
Animal-breeding.Saccharomycete is a kind of typical amphimicrobian type fungi, can adjust gastrointestinal function, inhibits pathogen.And ferment
Mother is able to maintain metabolic activity in gastrointestinal tract, ties up to a variety of Nutrition and Metabolism products of intestinal secretion, such as protein, fat, sugar and B race
Raw element etc., can also promote small intestine to absorb amino acid.Yeast cell wall internal layer contains glucan, these macromoleculars can excite non-specific
Property and specific immune response, activation T cell, B cell, macrophage and natural killer cells.Saccharomycete has tolerance thin simultaneously
The effect of mushroom antibiotic.But the factors such as strain, dosage and the application environment of saccharomycete have a significant impact function and effect, so
Ideal saccharomycete is preferably from the ancestral home bacterial strain of animal alimentary canal.Furthermore saccharomycete resistance is poor, such as non-refractory,
Acid is very strong to its lethality, intolerant to starvation etc., hinders its application in production practice.Although as mechanical processing technique
It continues to develop, using granulation, coating and low temperature drying technology have made application of the saccharomycete on feed industry have become possibility,
But the resistance of yeast is solved the problems, such as from strain, improve yeast cells heat resistance, the adaptation to gastrointestinal tract acidic environment
Property and hindgut environment nutrition starvation endurance be produce yeast source probiotics basic method.
Summary of the invention
It is that be the provision of a kind of resistance meat duck feeding present invention aims to overcome that the shortcomings that traditional yeast for animal feeds bacterium
Yeast strain, the bacterial strain are resistant to 25~42 DEG C of environment temperature, cultivate 24 hours at 42 DEG C, biomass 32.5g/L；Resistance to pH
Value 2.5~7.0 is cultivated 24 hours under the conditions of 3.0 pH, and biomass reaches 37.9g/L；Bile tolerance concentration 0~0.6%,
It is cultivated 24 hours under 0.3% fowl gallbladder salinity, biomass reaches 40.6g/L；With resistance to nutrition starvation 0~6 day, in nutrition starvation 6
Survival rate is up to 89.6% after it.Therefore the bacterial strain can survive and play one's part to the full in the stomach, enteron aisle of meat duck, can be effective
Substitute feeding antibiotic.
Another object of the present invention is to be the provision of a kind of resistance meat duck yeast for animal feeds bacterial strain in fleshy duck fodder
Application, added in meat duck basal diet the saccharomycete be main active ingredient group meat duck in growth performance, feed conversion
It is considerably better than basal feed group in terms of rate and the death rate, growth rate 7% is improved, reduces feed-weight ratio 0.04, death rate reduction
3.1%.And with addition antibiotic group without significant difference.Show that the saccharomycete can substitute feeding antibiosis in fleshy duck fodder
The use of element.
In order to achieve the above purpose, the present invention uses following technical measures:
A kind of preparation method of resistance meat duck yeast for animal feeds bacterial strain, the steps include:
After A takes the gastral content of meat duck to be diluted, plate streaking is carried out, is selected and conventional S. cervisiae form
Consistent bacterium colony further separates.
The S. cervisiae of acquisition is carried out heat resistance, acid resistance, resistance to nutrition starvation and bile tolerance and screened by B.
Isolated saccharomycete is carried out Physiology and biochemistry identification and 26s rDNA Molecular Identification by C.
Saccharomyces cerevisiae (Saccharomyces cerevisiae) WHY-7 in the present invention, send on October 10th, 2016
Preservation, deposit number CCTCC NO:M 2016550: address: Wuhan, China Wuhan are carried out toward China typical culture collection center
University.
Yeast strain WHY-7 in the present invention derives from meat duck alimentary canal, has typical saccharomyces cerevisiae morphological feature: institute
The cell for the saccharomyces cerevisiae stated is circle, and polygon budding, bacterium colony projection is smooth, milky, the typical wine brewing ferment such as neat in edge
Female morphological feature, also have following features: be resistant to 25~42 DEG C of environment temperature, pH value 2.5~7.0, gallbladder salinity 0~
0.6% and nutrition starvation 0~6 day.
The physical and chemical experiment of saccharomyces cerevisiae WHY-7 of the invention the results are shown in Table 1 ("+" represent can, "-" represents no).
The physical and chemical experiment result of 1 saccharomyces cerevisiae WHY-7 of table
The bacterial strain the most suitable growth pH is 5.0, but all has good activity between Ph 2.5-7.0, under the conditions of pH3.0
Culture 24 hours, biomass is calculated as 37.9g/L with thallus weight in wet base, hence it is evident that is higher than routine saccharomyces cerevisiae CCTCC AY 92003.One
As, for the pH of animal gastric juice 3.0 or so, this acidic environment condition has very strong lethal effect to conventional yeasts bacterium, and
Saccharomycete of the invention has very high activity under similar animal gastric fluid conditions.
The bacterial strain all has good growth activity under 0~0.6% fowl gallbladder salinity, in 0.3% fowl gallbladder salinity
Lower culture 24 hours, biomass is calculated as 40.6g/L with thallus weight in wet base, hence it is evident that is higher than routine saccharomyces cerevisiae CCTCC AY 92003.
Under normal circumstances, gallbladder salinity fluctuates in 0.03%~0.30% range in animal small intestine.After conventional yeasts bacterium enters enteron aisle,
The hyperosmosis environment of cholate is unfavorable for its survival in small intestine, and saccharomycete of the invention is in similar animal intestinal juice gallbladder salinity
Under the conditions of, there is very high activity.
30 DEG C of the bacterial strain optimum growth temperature, but heat resistance with higher and extensive thermal adaptability, at 25-45 DEG C
With good growth performance, 42 DEG C are cultivated 24 hours, and biomass is calculated as 32.5g/L with thallus weight in wet base, hence it is evident that are higher than conventional make
Biomass of the brewer yeast CCTCC AY 92003 (being purchased from China typical culture collection center, CCTCC) under similarity condition
7.2g/L。
The nutrition that the bacterial strain can be resistant to 0~8d is hungry, and survival rate is 89.6% after nutrition starvation 6d, hence it is evident that is higher than normal
Advise saccharomyces cerevisiae CCTCC AY 92003.In general the nutrition in animal hindgut often relatively lacks, and conventional yeasts bacterium is not easy to deposit
It is living, and saccharomycete of the invention can still have very high activity after the starvation of long period.
Yeast strain (Saccharomyces cerevisiae) WHY-7 provided by the invention is preserved in Chinese Typical Representative culture
Object collection, deposit number are CCTCC NO:M 2016550, and preservation date is on October 10th, 2016.Chinese Typical Representative culture
Object collection abbreviation CCTCC is located at Wuhan City, Hubei Province Wuhan University in the school, postcode 430072, phone: 027-
68752319, Email:cctcc@whu.edu.cn.
A kind of resistance meat duck yeast for animal feeds bacterial strain (probiotics containing saccharomycete WHY-7) is in fleshy duck fodder
Using process is:
A, Cherry Village Duckss are selected in experiment, if control group, antibiotic group and yeast group, every group of 5 repetitions, the culture-cycle is
35 days.
B, it weighs in experiment beginning and end, records feed food ration, record meat duck case fatality rate.
C, statistical experiment is as a result, carry out data processing.
Experiments have shown that: adding the probiotics containing yeast WHY-7 in meat duck basal diet can show compared with control group
It lands and improves the average daily gain of meat duck, and reduce feedstuff-meat ratio, while with addition antibiotic group without significant difference.Therefore, with ferment
Female bacterium WHY-7 is that the probiotics of main active can be used as the substitute of effective feeding antibiotic, to adjusting meat
Duck gastrointestinal microbiological group maintains intestinal flora balance, and improving production performance etc. has good facilitation.
Compared with prior art, the present invention having the following advantages that and effect:
1, yeast strain (Saccharomyces cerevisiae) WHY-7 provided by the invention is resistant to environment temperature
It 25~42 DEG C, is cultivated 24 hours at 42 DEG C, biomass 32.5g/L；Resistance to pH value 2.5~7.0 cultivates 24 under the conditions of 3.0 pH
Hour, biomass reaches 37.9g/L；Bile tolerance concentration 0~0.6% is cultivated 24 hours under 0.3% fowl gallbladder salinity, biology
Amount reaches 40.6g/L；With resistance to nutrition starvation 0~6 day, survival rate is up to 89.6% after nutrition hungry 6 days.Therefore the bacterial strain energy
It survives and plays one's part to the full in the stomach, enteron aisle of meat duck, can effectively substitute feeding antibiotic.
2, proved in fleshy duck fodder using result: addition is being grown with the meat duck that this saccharomycete is main active ingredient group
Be considerably better than basal feed group in terms of performance, efficiency of feed utilization and the death rate, can be improved growth rate 7%, reduce feed-weight ratio 0.04,
The death rate reduces 3.1%, and with addition antibiotic group without significant difference.Show that the saccharomycete can substitute in fleshy duck fodder
The use of feeding antibiotic.
Detailed description of the invention
Fig. 1 is a kind of bacterial strain WHY-7 and growth fraction of the routine saccharomyces cerevisiae CCTCC AY 92003 under condition of different pH
Compared with schematic diagram.
Wherein black column is bacterial strain WHY-7 biomass of the present invention, and white column is conventional saccharomyces cerevisiae CCTCCAY 92003
Biomass.
Fig. 2 is a kind of growth of bacterial strain WHY-7 and routine saccharomyces cerevisiae CCTCC AY 92003 in different fowl gallbladder salinities
Comparison schematic diagram.
Wherein black column is bacterial strain WHY-7 biomass of the present invention, and white column is conventional saccharomyces cerevisiae CCTCCAY 92003
Biomass.
Fig. 3 be a kind of bacterial strain WHY-7 and routine saccharomyces cerevisiae CCTCC AY 92003 at a temperature of growth compared with signal
Figure.
Wherein black column is bacterial strain WHY-7 biomass of the present invention, and white column is conventional saccharomyces cerevisiae CCTCCAY 92003
Biomass.
Fig. 4 is the 26s rDNA sequence PCR amplification electrophoresis schematic diagram of yeast strain WHY-7 a kind of.
Wherein swimming lane M is DNA molecular amount standard, and swimming lane 26s is produced for the 26s rDNA gene order PCR amplification of WHY-7
Object.
Fig. 5 is the 26s for the saccharomyces cerevisiae reported in 26s rDNA sequence in a kind of yeast strain WHY-7 and GenBank
RDNA sequence homology comparison schematic diagram.
Wherein KM655848.1 is the saccharomyces cerevisiae 26s rDNA sequence reported in GenBank, and WHY-7 is bacterial strain of the present invention
26s rDNA sequence in WHY-7.
Specific embodiment
The present invention is further explained in the light of specific embodiments, but the present invention is not limited to following embodiments.
In following embodiments, unless otherwise specified, used experimental method is conventional method；Experiment reagent and material used
Material can be obtained through commercial channels.
Embodiment 1:
A kind of preparation method of resistance meat duck yeast for animal feeds bacterial strain Saccharomyces cerevisiae WHY-7,
Step is:
A, the separation and screening of bacterial strain:
(1) anatomical acquisition meat duck alimentary canal content, after diluting 10 times with sterile saline, with rubbing method in brewer's wort
2 or 3d is cultivated on agar plate under the conditions of 30 DEG C, picking and periphery bacterium colony have the single colonie of certain distance further to be drawn with plate
Collimation method purifies 2 or 3 times.The thallus of one ring of picking after purification is inoculated in YPD (containing yeast extract in every 1000ml culture medium later
10g, peptone 20g, glucose 20g, agar powder 20g) on slant medium, 30 DEG C of 2 or 3d of culture in constant incubator.
(2) smooth, milky, side by the surface similar with conventional saccharomyces cerevisiae bacterium colony grown in solid medium tablets
The neat single colonie of edge is connected to the inclined-plane YPD, 30 DEG C stationary culture 48 hours.Microscopically observation cellular morphology and size, pass through
It is compared with conventional brewing yeast cell, obtains oval cell, polygon budding, size and conventional saccharomyces cerevisiae basic one
The single colonie of cause.Obtain 7 Accharomyces cerevisiae bacterium altogether, respectively name WHY-1, WHY-2, WHY-3, WHY-4, WHY-5, WHY-6 and
WHY-7。
(3) a ring somatic cells are connect respectively in 2ml liquid YPD (agar powder is not added) from the inclined-plane YPD, 30 DEG C of shaking tables (200
Rev/min) culture 18 hours, then the 10ml YPD liquid that pH is 3.0 is transferred to by the inoculum concentration that percent by volume is 10%
In culture medium, 30 DEG C (200 revs/min) of shaking table are cultivated 24 hours, are centrifuged (5000 revs/min, 5 minutes) collection thallus, thallus
Cell is primary with distillation washing, is as above centrifuged, and weighs to cell precipitation, calculate cellular biomass (g/L, it is wet in every liter of fermentation liquid
Cell weight).Experiment in triplicate, while being control with conventional saccharomyces cerevisiae CCTCC AY 92003.It is separated to from above-mentioned 7 plants
In single colonie obtain three under the conditions of pH3.0 cellular biomass be higher than control saccharomyces cerevisiae bacterial strain, respectively WHY-7,
WHY-2 and WHY-5.
(4) a ring somatic cells are connect respectively in 2ml liquid YPD from the inclined-plane YPD of WHY-7, WHY-2 and WHY-5,30 DEG C
(200 revs/min) of shaking table are cultivated 18 hours, are then transferred to the training of 10ml YPD liquid by the inoculum concentration that percent by volume is 10%
It supports in base, is cultivated 24 hours at (200 revs/min) of 37 DEG C of shaking tables, be centrifuged (5000 revs/min, 5 minutes) collection thallus, thallus
Cell is primary with distillation washing, is as above centrifuged, and weighs to cell precipitation, calculates cellular biomass.It tests in triplicate, while with
Conventional saccharomyces cerevisiae CCTCC AY 92003 is control.As a result under the above conditions, bacterial strain WHY-7 shows good growth
Can, cellular biomass is apparently higher than control Wine brewing yeast strain and bacterial strain WHY-2 and WHY-5.
B, the physiological property of bacterial strain WHY-7:
(1) acid resistance: by bacterial strain WHY-7 and conventional saccharomyces cerevisiae CCTCC AY 92003 as control respectively in 5ml
It cultivates 18 hours for 30 DEG C (200 revs/min) of shaking table in liquid YPD, is then transferred to by the inoculum concentration that percent by volume is 10%
45ml is cultivated 24 hours in the YPD fluid nutrient medium of different pH (2.5-7.0) at (200 revs/min) of 30 DEG C of shaking tables.Such as
Upper thalline were collected by centrifugation, and weighing calculates biomass.Experiment is in triplicate.The result shows that the bacterial strain WHY-7 most adaptability screened
Long pH is 5.0, but good activity is all had between pH 2.5-7.0, is cultivated 24 hours under the conditions of pH 3.0, biomass reaches
It is 1.9 times (Fig. 1) of conventional 92003 biomass of saccharomyces cerevisiae CCTCC AY to 37.9g/L.
(2) Bile salt resistance: bacterial strain WHY-7 and the conventional saccharomyces cerevisiae CCTCC AY92003 as control are existed respectively
It cultivates 18 hours for 30 DEG C (200 revs/min) of shaking table in 5ml liquid YPD, then transfers by the inoculum concentration that percent by volume is 10%
In the YPD fluid nutrient medium to 45ml with (0%-0.6%) of various concentration fowl cholate, at 30 DEG C of shaking tables (200 revs/min)
Culture 24 hours.As above thalline were collected by centrifugation, and weighing calculates biomass.Experiment is in triplicate.The result shows that bacterial strain WHY-7 exists
It grows best when not adding fowl cholate, but all has good growth activity under 0%-0.6% fowl gallbladder salinity,
It is cultivated 24 hours under 0.3% fowl gallbladder salinity, biomass reaches 40.6g/L, hence it is evident that be higher than saccharomyces cerevisiae CCTCC AY 92003
(Fig. 2).
(3) heat resistance: by bacterial strain WHY-7 and conventional saccharomyces cerevisiae CCTCC AY 92003 as control respectively in 5ml
It cultivates 18 hours for 30 DEG C (200 revs/min) of shaking table in liquid YPD, is then transferred to by the inoculum concentration that percent by volume is 10%
In 45ml YPD fluid nutrient medium, respectively in 30 DEG C, 33 DEG C, 37 DEG C, 40 DEG C, 42 DEG C and 45 DEG C (200 revs/min) of shaking table cultures
24 hours.As above thalline were collected by centrifugation, and weighing calculates biomass.Experiment is in triplicate.Fig. 3 is the result shows that the bacterial strain screened
WHY-7 have 30 DEG C of optimum growth temperature identical with conventional saccharomyces cerevisiae, but bacterial strain WHY-7 have better heat resistance, 42
DEG C culture 24 hours, biomass 32.5g/L, hence it is evident that higher than conventional saccharomyces cerevisiae CCTCC AY 92003 under similarity condition
Biomass 7.2g/L；45 DEG C are cultivated 24 hours, and bacterial strain WHY-7 biomass is 18.5g/L, and routine saccharomyces cerevisiae CCTCC AY
92003 cannot grow completely at 45 DEG C.
By bacterial strain WHY-7 and routine saccharomyces cerevisiae CCTCC AY 92,003 30 DEG C of shaking tables (200 in 5ml liquid YPD respectively
Rev/min) culture 18 hours, 5000 revs/min of centrifugations, 5 minutes collection thallus are claimed with being as above centrifuged after sterile water washing thalline
Thallus, is resuspended in sterile water in 0.05g/ml wet thallus ratio, takes 0.5ml bacteria suspension to be added to 4.5ml sterile by weight
In water, 80 DEG C of incubation processing were coated on YPD solid plate, 30 DEG C quiet after carrying out gradient dilution every 10 seconds sampling 0.2ml
Culture 48 hours is set, clump count is calculated.Three repetitions are set every time, and experiment is in triplicate.According to the clump count of different disposal time
Calculate survival rate, it may be assumed that survival rate (%)=((clump count × extension rate of different disposal time)/(processing starting clump count)
× extension rate)) × 100.The result shows that bacterial strain WHY-7 is handled 20 seconds at 80 DEG C, survival rate is maintained at 78.5%, processing 30 seconds
Survival rate is able to maintain 64.9%, and as the conventional saccharomyces cerevisiae CCTCC AY 92003 of control 80 DEG C handle 20 seconds it is complete
Lose activity (table 1) entirely.
The heat resistance of 1 bacterial strain WHY-7 of table and routine saccharomyces cerevisiae CCTCC AY 92003 compares
(4) nutrition starvation endurance: by bacterial strain WHY-7 and routine saccharomyces cerevisiae CCTCC AY 92003 respectively in 5ml liquid
It is cultivated 18 hours for 30 DEG C (200 revs/min) of shaking table in body YPD, 5000 revs/min of centrifugations, 5 minutes collection thallus, with sterile washing
It is as above centrifuged after washing thallus, collects thallus, wash 3 times with aseptic deionized water, again suspension thalline to the sterile deionization of 50mL
In aqueous solution, the concentration of thallus is made to reach 108Cell/mL is sampled in 30 DEG C of cultures on 200 revs/min of shaking tables every 48h, is fitted
YPD plate is coated with after dilution, 30 DEG C of culture 72h calculate clump count.Using initial time as control, cell survival rate is calculated,
Its nutrition starvation endurance is characterized with cell survival rate.The nutrition Starvation sexuality of thallus indicates with survival rate, survival rate
(%)=(Ct/C0) × 100%, C0And CtRespectively indicate the remaining viable cell concentrations that incubation time is 0 and t hours.2 result of table
Showing bacterial strain WHY-7, survival rate is up to 89.6% after nutrition is 6 days hungry, and there are also 51.2% for survival rate of the starvation after 10 days, and
Conventional saccharomyces cerevisiae CCTCCAY 92003 is then all dead after nutrition is 8 days hungry.
The nutrition starvation endurance of 2 bacterial strain WHY-7 of table and routine saccharomyces cerevisiae CCTCC AY 92003 compares
C, the identification of saccharomycete:
From connecing a ring bacterial strain WHY-7 cell on the inclined-plane YPD in 2ml YPD fluid nutrient medium, 30 DEG C (200 revs/min of shaking table
Clock) culture 18 hours.50 μ l bacterium solutions are taken to be connected in 2ml sterile water, 30 DEG C (200 revs/min) of shaking table are cultivated 5 hours；Respectively take 50 μ
The above-mentioned bacteria suspension of l, be connected to equipped with 3.5ml using variety classes sugar or alcohol as carbon source yeast minimal medium (0.67%YNB, 2%
Different types of sugar or alcohol, natural pH) in, wherein detecting sugared fermentation gas situation equipped with inverted Du Shi is effective, 30 DEG C quiet
Set culture, every 24 hours observation fermentation gas situations.The results show that bacterial strain WHY-7 can with glucose fermentation, maltose, sucrose,
Galactolipin and gossypose, but unfermentable lactose, salicin, cellobiose, citrate, xylitol and methanol etc..According to bacterium
Assimilation and fermentation character of the strain to different carbon source, tentatively judge that bacterial strain WHY-7 may belong to saccharomyces cerevisiae.
Since the D1/D2 sector sequence of the 26Sr DNA in saccharomyces cerevisiae (Saccharomyces cerevisiae) is height
Conserved region is spent, therefore chooses the identification that this section of sequence carries out molecular level to bacterial strain WHY-7.According to the 26Sr reported in GenBank
D1/D2 sector sequence (KM655848) design and synthesis primer of DNA is as follows:
Upstream primer NL1:5'-GCATATCAATAAGCGGAGGAAAAG-3'
Downstream primer NL4:5'-GGTCCGTGTTTCAAGACGG-3'
The genomic DNA for extracting WHY-7 bacterial strain, as template, using PCR method to the 26S rDNA for obtaining saccharomycete
D1/D2 section fragment is expanded and is sequenced.PCR reaction system is 50 μ L, and 10 × Taq Buffer, 5.0 μ L is added in every pipe,
2.5mol/L dNTP 1.0 μ L of 4.0 μ L, each 1.0 μ L of 20 μm of ol/L upstream and downstream primers, 10 μ L, 2.5U/ μ L Taq of template DNA,
28.0 μ L of sterile water.Reaction condition: 94 DEG C of initial denaturation 5min；94 DEG C of denaturation 45s；55 DEG C of annealing 60s；72 DEG C of extension 70s, altogether into
30 PCR cycles of row, finally again 72 DEG C continue extend 10min, 4 DEG C of preservations.Agarose gel electrophoresis detects PCR as a result, discovery
The DNA fragmentation that about 650bp can be amplified using bacterial strain WHY-7 genomic DNA as template, it is (Fig. 4) in the same size with expection.To bacterium
The wine brewing ferment reported in pcr amplification product (about 650bp) the progress sequence analysis and comparison of strain WHY-7, discovery and Gen Bank
The 26S rDNA D1/D2 section fragment sequence identity of female (Saccharomyces carevisiae) is 99.68% (Fig. 5).
It is above-mentioned the experimental results showed that, bacterial strain WHY-7 should be accredited as saccharomyces cerevisiae.
The bacterial strain WHY-7 that the present invention screens is preserved in Chinese microorganism strain preservation management on October 10th, 2016
Committee's common micro-organisms center (abbreviation CCTCC, address are as follows: Wuhan City, Hubei Province Wuhan University is in the school), specific name are to make
Brewer yeast (Saccharomyces cerevisiae), deposit number are CCTCC NO:M 2016550.
The bacterial strain has typical saccharomyces cerevisiae morphological feature: cell is ellipse, polygon budding；Bacterium colony projection, it is smooth,
Milky, neat in edge；With typical saccharomyces cerevisiae physiological and biochemical property, glucose fermentation, maltose, sucrose, galactolipin
And gossypose, but unfermentable lactose, salicin, cellobiose, citrate, xylitol and methanol etc..But than conventional wine brewing
Yeast shows the performance of higher heat-resisting, acidproof, bile tolerance and resistance to starvation.
Embodiment 2:
A kind of resistance meat duck yeast for animal feeds bacterial strain (probiotics containing saccharomycete WHY-7) is in fleshy duck fodder
Using the steps include:
A, 1 age in days Cherry Village Duckss, 450 plumage is selected, is randomly divided into 3 groups, every group of 5 repetitions, every 30 plumage of repetition.
B, test duck free-ranging under indoor same environment.Each 1 column of repetition, for 24 hours illumination are freely eaten and drink water, with weight
Feed injected volume and surplus are recorded daily for unit again, clean water fountain and hopper 1 time daily.
C, immunity inoculation and disease prevention, disinfection carry out according to a conventional method.Experiment is divided into control group (basal feed), antibiosis
Plain group (basal feed+4mg flavomycoin), yeast group (every kg basal feed+1 × 109A yeast cells).Experimental period is 35d.It is real
Testing place is farm, Wuhan City's immortality Ya Ye company.Cherry Village Duckss were carried out on an empty stomach respectively in test the 1st day and the 35th day
Weighing calculates counterpoise, average daily gain and feed-weight ratio.
The experimental results showed that adding yeast in feed compared with control group, the rate of body weight gain of Cherry Village Duckss can be significantly improved, reduced
Feed-weight ratio and case fatality rate, but with antibiotic group without significant difference.Therefore, this strain can be made in fleshy duck fodder with substitute antibiotics
With.
Influence of the yeast to cherry village ducks production performance is added in 3 feed of table
| Group | Average daily gain (g) | Feed-weight ratio | Case fatality rate (%) |
| Control group | 57.49 | 2.17 | 4.5 |
| Antibiotic group | 61.38 | 2.13 | 2.5 |
| Yeast group | 61.52 | 2.13 | 1.4 |
Claims (2)
1. a kind of high activity meat duck tailored version yeast for animal feeds bacterial strain, it is characterised in that: the bacterial strain is saccharomyces cerevisiae
(Saccharomyces cerevisiae) WHY-2, CCTCC NO:M 2016549.
2. a kind of application of the saccharomyces cerevisiae described in claim 1 in fleshy duck fodder.
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