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CN106396958A - Preparation method and application of compound preparation for controlling physiological diseases of apples - Google Patents

Preparation method and application of compound preparation for controlling physiological diseases of apples Download PDF

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Publication number
CN106396958A
CN106396958A CN201610812420.9A CN201610812420A CN106396958A CN 106396958 A CN106396958 A CN 106396958A CN 201610812420 A CN201610812420 A CN 201610812420A CN 106396958 A CN106396958 A CN 106396958A
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China
Prior art keywords
preparation
apple
spray
chlorella
nitric acid
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Application number
CN201610812420.9A
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Chinese (zh)
Inventor
韩明三
李贇
王芝云
刘学才
王珍青
孙吉录
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Priority to CN201610812420.9A priority Critical patent/CN106396958A/en
Publication of CN106396958A publication Critical patent/CN106396958A/en
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    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G5/00Fertilisers characterised by their form
    • C05G5/20Liquid fertilisers
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G13/00Protecting plants
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00Botany in general
    • A01G7/06Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N61/00Biocides, pest repellants or attractants, or plant growth regulators containing substances of unknown or undetermined composition, e.g. substances characterised only by the mode of action
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/10Animals; Substances produced thereby or obtained therefrom
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F1/00Fertilisers made from animal corpses, or parts thereof
    • C05F1/005Fertilisers made from animal corpses, or parts thereof from meat-wastes or from other wastes of animal origin, e.g. skins, hair, hoofs, feathers, blood
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Environmental Sciences (AREA)
  • Zoology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Dentistry (AREA)
  • Plant Pathology (AREA)
  • Agronomy & Crop Science (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Ecology (AREA)
  • Forests & Forestry (AREA)
  • Microbiology (AREA)
  • Virology (AREA)
  • Biotechnology (AREA)
  • Toxicology (AREA)
  • Environmental & Geological Engineering (AREA)
  • Botany (AREA)
  • Medicines Containing Plant Substances (AREA)

Abstract

The invention discloses a preparation method and application of a compound preparation for controlling physiological diseases of apples. The preparation method includes (1), respectively cleaning surfaces of oyster shells and abalone shells, drying and grinding the cleaned oyster shells and abalone shells, and sieving ground powder via a 200-mesh sieve; (2), mixing the ground oyster shells and the ground abalone shells in a mass ratio of 2 to 1; (3), adding 50% nitric acid to the mixed shell powder with stirring evenly, treating the mixture for 24 hours, filtering and collecting to obtain filtrate with the total salt content of about 55%, wherein the mass of the 50% nitric acid is 1.8-2.0 times of that of the mixed shell powder; (4), collecting a culture solution without chlorella, mixing the culture solution with the filtrate, and regulating the total salt content to be about 40% so as to obtain the compound preparation for controlling the physiological diseases of the apples. The preparation method and application of the compound preparation for controlling the physiological diseases of the apples have the advantages that the shells are aquaculture by-products and the chlorella culture solution is waste liquid discharged by a chlorella farms, and accordingly turning waste into wealth is achieved and a remarkable environmental protection effect is achieved; the compound preparation is mainly absorbed by leaves, and the residual compound preparation seeps into soil along with rain wash and can be absorbed by apple root systems, so that the purpose of controlling the physiological diseases of the apples can be achieved.

Description

A kind of preparation method and application of the compound formulation of preventing and treating apple physiology sexually transmitted disease (STD) evil
Technical field
The present invention relates to a kind of fruit diseases control agent, it is used for preventing and prevent and treat apple development particularly to one kind and developed Compound formulation of physiological disturbance occurring in journey and preparation method and application.
Background technology
Apple physiology sexually transmitted disease (STD) evil is to lead to one of important disease of apple development exception, fruit output and quality decline.As Fruit bitter pit, Petiole Spot etc. can make fruit lose value, and yellowing leaf disease, little leaf affect the growth promoter of tree body, sternly Severe one can not form economic flow rate completely, and therefore, the preventing and treating of physiological disturbance is the important content of orchard management.Although apple physiology Sexually transmitted disease (STD) evil performance symptom difference is very big, and the extent of injury differs, but is currently known that most of apple physiology sexually transmitted disease (STD)s evil is many and orchard soil Earth Mineral Elements Content and tree body are directly relevant to absorbing of mineral element.Therefore, prepare mineral element abundant species, contain Measure the effective way that compound formulation sufficient and easy to use is preventing and treating apple physiology sexually transmitted disease (STD) evil.
How shell is made up of the organic principle such as mineral and protein and polysaccharide.Mineral types are enriched, such as Concha Ostreae calcium Content up to more than 37%, also contains magnesium simultaneously(Content is 870mg/kg), ferrum(Content is 270mg/kg), zinc(Content is 80mg/kg), strontium(Content is 63mg/kg)Deng mineral element.Abalone shell calcium content is up to 40%, and magnesium and strontium are respectively 1.5% He 0.18%, main trace element also includes ferrum (content is 245mg/kg), zinc(Content is 152mg/kg).These elements are all right Preventing and treating apple physiology sexually transmitted disease (STD) does harm to effective mineral element.As calcium can prevent and prevents and treats Fructus Mali pumilae bitter pit, ferrum can prevent and prevent and treat Herba Marsileae Quadrifoliae Fruit yellowing leaf disease, zinc can prevent and prevent and treat Apple little leaf, and magnesium can prevent the speckle aetiolation of Apple Leaves, calcium, ferrum, zinc Synthesis lack and imbalance can cause fruit Petiole Spot, and strontium can promote the absorption to potassium, magnesium and ferrum for the fruit tree.
Using the mineral element preventing and treating apple physiology sexually transmitted disease (STD) evil in shell, Major Difficulties are how to realize Fructus Mali pumilae can be effective Absorb the mineral element in shell.And at present to shell using being only that shell is pulverized, this processing method is more difficult Effective ingredient effectively in release shell, the action time of preventing and treating apple physiology sexually transmitted disease (STD) evil aspect is slow, efficiency is low.
Content of the invention
For the problems referred to above, it is an object of the invention to provide a kind of system of the compound formulation of preventing and treating apple physiology sexually transmitted disease (STD) evil Preparation Method and its application, improve the utilization ratio of the wherein mineral element of shell.
The present invention seeks to being achieved through the following technical solutions:(1)It is respectively washed Concha Ostreae and abalone shell surface, dry rear powder Broken, cross 200 eye mesh screens;(2)After pulverizing, Concha Ostreae and abalone shell are pressed quality 2 to 1 and are mixed;(3)Quality pressed by mixed conch meal The 1.8-2.0 times of nitric acid adding 50% stir, process 24 hours, filter, collect and obtain total salinity and reach 55% about Filtrate;(4)Collect the culture fluid of reject chlorella, the filtrate being obtained with above-mentioned process is mixed, adjustment mixed liquor saliferous is total Amount 40% about, that is, obtains the compound formulation of described preventing and treating apple physiology sexually transmitted disease (STD) evil.
Described nitric acid is industrial nitric acid or chemical pure nitric acid.
The culture of described chlorella adopts common BG-11 micro-algae culture medium, and its composition is on the books in the literature.
Abundant for guaranteeing the organic principle in bead algae culturing liquid, the bead algae culturing liquid being used is continuous at least 3 batches Secondary culture chlorella cells concentration is to 107The culture fluid of/more than ml.
Above-mentioned compound formulation prevents and treats the application of apple physiology sexually transmitted disease (STD) evil, comprises the following steps:(1)Above-mentioned compound formulation is pressed Ratio adds water, salinity 0.1%-0.5% of adjustment spray liquid;(2)In apple flower one week after foliage-spray once, early June Spray again once, the generation of Fructus Mali pumilae bitter pit can be prevented completely;(3)Can prevent for 2 times in late May and foliage-spray mid-June The yellowing leaf being caused due to soil alkaline with preventing and treating;In Fructus Mali pumilae foliage-spray mid-July, can effectively prevent late July to 8 The apple leaf yellow that rainy season in month causes;(4)It is diluted with water to 0.5% foliage-spray and spend latter two during Fructus Mali pumilae germinates It is diluted to 0.3% foliage-spray in week, can effectively prevent and prevent and treat Apple little leaf and fruit Petiole Spot;(5)In apple flower It is diluted to 0.3% foliage-spray in latter two week, be diluted to 0.2% mid-June and carry out foliage-spray, can effectively prevent and cure Apple Leaves speckle yellows.
The invention has the beneficial effects as follows:The mixing of two kinds of shells is so that the mineral element species of compound formulation and main The content of mineral element more horn of plenty;The shell that nitric acid treatment is pulverized, can decompose two kinds of shells, the release overwhelming majority therein ore deposit Prime element, pulverizes shell using nitric acid rather than salt acid degradation, not only can reach the purpose of degraded shell, also can supplement certain Amount nitrate nitrogen and do not increase the absorption of the chloride ion unfavorable to fruit tree;Add bead algae culturing liquid to mainly use chlorella to exist Secrete in incubation to the small organic molecule of culture fluid, these Organic substances do not contain only substantial amounts of free amino acid, polysaccharide, The also bioactive ingredients containing abundant species, these compositions not only contribute to the slow release of shell mineral element and absorption is fixed, Improve the absorption efficiency to free mineral element for the Fructus Mali pumilae, also there is the effect promoting growth and development of fruit tree.
Shell used by the present invention is aquaculture side-product, and the bead algae culturing liquid being used discharges for chlorella plant Waste liquid, such use can be turned waste into wealth, and has obvious environmental protection effect;Made compound formulation is except being absorbed by blade Outward, remainder can enter soil with rain drop erosion or root system of the apple absorbs, and equally can reach prevents apple physiology The purpose of sexually transmitted disease (STD) evil.
Specific embodiment
Below by specific embodiment, the present invention is described in detail.
Embodiment 1:
With Concha Ostreae and abalone shell as raw material, surface clean, mechanical activation comminution respectively, cross 200 eye mesh screens, 2 to 1 mixing, after pulverizing 1.8 times of addition 50% industrial nitric acid of shell quality, stir, process 24 hours;Filter, collect filtrate;Add chlorella Culture fluid is mixed, and keeps mixed liquor saliferous total amount 40% about, that is, obtain the compound of described preventing and treating apple physiology sexually transmitted disease (STD) evil Preparation.During use, the adjustment total salinity that adds water is 0.4%, and in apple flower one week after, once, early June sprays foliage-spray again Once, the generation of Fructus Mali pumilae bitter pit can be prevented completely, and the apple tree bitter pit compareing spray clear water occurs in 8-25%(Not the same year Part sickness rate is different).Draw and use on Fuji apple in loud, high-pitched sound simultaneously, loud, high-pitched sound Fructus Mali pumilae soluble solids also can be made averagely to improve 0.5%, make Fuji apple soluble solids averagely improve 0.8%.
Embodiment 2:
With Concha Ostreae and abalone shell as raw material, surface clean, mechanical activation comminution respectively, cross 200 eye mesh screens, 2 to 1 mixing, after pulverizing 2.0 times of chemical pure nitric acid of addition of shell quality, stir, process 24 hours;Filter, collect filtrate;Add chlorella training Nutrient solution and a certain amount of water final total salinity of adjustment are 0.3%, late May and foliage-spray mid-June can prevent for 2 times and Prevent and treat the yellowing leaf causing due to soil alkaline.In Fructus Mali pumilae foliage-spray mid-July, can effectively prevent late July to August The apple leaf yellow that part rainy season causes.
Embodiment 3:
With Concha Ostreae and abalone shell as raw material, surface clean, mechanical activation comminution respectively, cross 200 eye mesh screens, 2 to 1 mixing, after pulverizing 1.8 times of chemical pure nitric acid of addition of Concha Ostreae quality, stir, process 24 hours;Filter, collect filtrate;Add chlorella Culture fluid, is mixed, and keeps mixed liquor saliferous total amount 40% about, that is, obtains answering of described preventing and treating apple physiology sexually transmitted disease (STD) evil Close preparation.It is diluted with water to total salinity 0.5% foliage-spray during Fructus Mali pumilae germinates, be diluted with water in latter two week of flower 0.3% foliage-spray.Can effectively prevent and prevent and treat Apple little leaf and fruit Petiole Spot, and Fuji apple solubility can be put forward Solid content 0.8%.
Embodiment 4:
With Concha Ostreae and abalone shell as raw material, surface clean, mechanical activation comminution, cross 200 eye mesh screens, by Concha Ostreae quality after pulverizing 1.8 times of chemical pure nitric acid of addition, stir, process 24 hours;Filter, collect filtrate;Bead algae culturing liquid is added to be mixed Close, keep mixed liquor saliferous total amount 40% about, that is, obtain the compound formulation of described preventing and treating apple physiology sexually transmitted disease (STD) evil.In Fructus Mali pumilae It is diluted with water to total salinity 0.3% foliage-spray in latter two week of flower, be diluted to 0.2% mid-June and carry out foliage-spray, can have The prevention of effect and healing Apple Leaves speckle yellows.
Above-described embodiment only technology design to illustrate the invention and feature, can not limit the protection model of the present invention with this Enclose.All equivalent transformations being done according to spirit of the invention or modification, all should cover within the scope of the present invention.

Claims (5)

1. a kind of preparation method of the compound formulation of preventing and treating apple physiology sexually transmitted disease (STD) evil is it is characterised in that comprise the following steps:(1)Point Not Qing Xi Concha Ostreae and abalone shell surface, after drying pulverize, cross 200 eye mesh screens;(2)After pulverizing, Concha Ostreae and abalone shell press quality 2 Than 1 mixing;(3)Mixed conch meal stirs by 1.8-2.0 times of the quality nitric acid adding 50%, processes 24 hours, mistake Filter, collects and obtains the filtrate that total salinity reaches 55% about;(4)Collect the culture fluid of reject chlorella, with above-mentioned process acquisition Filtrate mixes, and adjustment mixed liquor saliferous total amount 40% about, that is, obtains the compound formulation of described preventing and treating apple physiology sexually transmitted disease (STD) evil.
2. preparation method according to claim 1 is it is characterised in that described nitric acid is industrial nitric acid or chemical pure nitric acid.
3. preparation method according to claim 1 is it is characterised in that the culture of described chlorella adopts BG-11 microdisk electrode Base.
4. preparation method according to claim 1 is it is characterised in that described bead algae culturing liquid is continuous at least 3 batch trainings Foster chlorella cells concentration is to 107The culture fluid of/more than ml.
5. compound formulation prevents and treats the application of apple physiology sexually transmitted disease (STD) evil according to claim 1, comprises the following steps:(1)By institute State compound formulation to add water in proportion, salinity 0.1%-0.5% of adjustment spray liquid;(2)Foliage-spray in apple flower one week after Once, early June sprays once again, can prevent the generation of Fructus Mali pumilae bitter pit completely;(3)In late May and blade face spray mid-June Apply 2 times and can prevent and prevent and treat the yellowing leaf causing due to soil alkaline;In Fructus Mali pumilae foliage-spray mid-July, can be effectively pre- The apple leaf yellow that anti-late July caused to August part rainy season;(4)It is diluted with water to 0.5% blade face spray during Fructus Mali pumilae germinates Apply and in spending latter two week, be diluted to 0.3% foliage-spray, can effectively prevent and prevent and treat Apple little leaf and fruit pore Disease;(5)It is diluted to 0.3% foliage-spray within latter two week of apple flower, be diluted to 0.2% mid-June and carry out foliage-spray, can have The prevention of effect and healing Apple Leaves speckle yellows.
CN201610812420.9A 2016-09-09 2016-09-09 Preparation method and application of compound preparation for controlling physiological diseases of apples Pending CN106396958A (en)

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Application Number Priority Date Filing Date Title
CN201610812420.9A CN106396958A (en) 2016-09-09 2016-09-09 Preparation method and application of compound preparation for controlling physiological diseases of apples

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113197225A (en) * 2021-04-30 2021-08-03 上海交通大学 Resource utilization method of polyphenol waste liquid from fucoxanthin extraction from brown algae
CN113999068A (en) * 2021-11-26 2022-02-01 北海宝农农业科技有限公司 Method for producing full water-soluble sugarcane yield-increasing and sugar-increasing fertilizer by using marine wastes

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103232303A (en) * 2013-04-23 2013-08-07 韩明三 Special-purpose fruit tree biological organic fertilizer and preparation method thereof
CN105087016A (en) * 2015-09-09 2015-11-25 南宁汉和生物科技有限公司 Soil conditioner rich in marine substances

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103232303A (en) * 2013-04-23 2013-08-07 韩明三 Special-purpose fruit tree biological organic fertilizer and preparation method thereof
CN105087016A (en) * 2015-09-09 2015-11-25 南宁汉和生物科技有限公司 Soil conditioner rich in marine substances

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113197225A (en) * 2021-04-30 2021-08-03 上海交通大学 Resource utilization method of polyphenol waste liquid from fucoxanthin extraction from brown algae
CN113999068A (en) * 2021-11-26 2022-02-01 北海宝农农业科技有限公司 Method for producing full water-soluble sugarcane yield-increasing and sugar-increasing fertilizer by using marine wastes

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Application publication date: 20170215

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