CN106344966A - Method for improving cell adhesion of polyglycolic acid stent by using mesoporous silica - Google Patents
Method for improving cell adhesion of polyglycolic acid stent by using mesoporous silica Download PDFInfo
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- CN106344966A CN106344966A CN201510408546.5A CN201510408546A CN106344966A CN 106344966 A CN106344966 A CN 106344966A CN 201510408546 A CN201510408546 A CN 201510408546A CN 106344966 A CN106344966 A CN 106344966A
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- polyglycolic acid
- powder
- silicon oxide
- cell adhesion
- mesoporous silica
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Abstract
The invention discloses a method for improving the cell adhesion of a composite stent by preparing the composite stent by adding a small amount of mesoporous silica with high specific surface area, large pore volume and proper aperture into polyglycolic acid under a selective laser sintering process. Meanwhile, mesoporous silica particles serving as crosslinking points and the polyglycolic acid form a meshed crosslinking structure, so that the intensity and the hardness of the stent are improved; silicon is a trace element necessary to a human body, so that collagen synthesis and bone matrix calcification can be promoted; during repairing of bone tissue damage or deficiency, silicon plays a very important regulation role in formation of new bones.
Description
Technical field
The present invention relates to a kind of improved the cell adhesion performance of polyglycolic acid bone support, carry using mesopore silicon oxide simultaneously
Its mechanical performance high and the method for biological activity, belong to bone tissue engineer field.
Background technology
Polyglycolic acid (also known as Poly-L-lactic acid) is U.S.'s food and Drug Administration's certification and ratifies can be applicable to
The biomaterial of Tissue Engineering Study, has good biocompatibility and degradability, higher toughness plasticity, easily
The advantages of processing, it is widely used to field of tissue engineering technology at present.But find in application process its exist intensity and
Hardness is not enough, and cell adhesion ability and the poor shortcoming of biological activity, greatly limit it further
Application.
Mesopore silicon oxide is a kind of degradable biomaterial, because its have higher specific surface area (specific surface area 600～
800m2/ g), larger pore volume (pore volume 1.02m3/ g) and orderly meso-hole structure (mesopore diameter 6～11nm),
And pore size can be kept homogeneous in bone support preparation process, duct is regularly arranged and is interconnected, and these are intrinsic
Characteristic makes it have preferable hydrophilic and stronger absorbability, roomy surface area and space, and this is conducive to cell
Adhesion growth, nutrient substance and oxygen enter and metabolite is discharged, and be also beneficial to blood vessel and nerve is grown into.
In sum, the present invention passes through to add mesopore silicon oxide to form composite powder, using selectivity in polyglycolic acid
Laser sintering technology prepares mesopore silicon oxide/polyglycolic acid compound rest, and mesopore silicon oxide therein can improve the thin of support
Born of the same parents' adhesive capacity, simultaneously mesopore silicon oxide granule formed as crosslinking points and polyglycolic acid and there is cross-linked network, energy
Improve intensity and the hardness of support；Additionally, the element silicon included in mesopore silicon oxide is trace element needed by human,
Collage synthesis and calcified bone matrix can be promoted, the formation to new bone plays highly important regulating and controlling effect.
Content of the invention
The present invention passes through to add mesopore silicon oxide formation composite powder in polyglycolic acid, and utilizes selective laser sintering
Technology prepares mesopore silicon oxide/polyglycolic acid compound rest, its objective is there is high-specific surface area, big pore volume by interpolation
To improve the cell adhesion ability of support with the mesopore silicon oxide in suitable aperture, mesopore silicon oxide granule is as crosslinking simultaneously
Point is formed with polyglycolic acid has cross-linked network, improves intensity and the hardness of support；Additionally, silicon be human body must
The trace element needing, can promote collage synthesis and calcified bone matrix, and the formation to new bone plays highly important regulation and control work
With.
Embodiment of the present invention includes:
The preparation process of mesopore silicon oxide/polyglycolic acid compound rest:
A) weigh 5% mesopore silicon oxide respectively by mass fraction and 95% polyglycolic acid powder carries out simple and mechanical mixing
Close；
B) load weighted for step a) mixed-powder is added in a certain amount of anhydrous alcohol, abundant using magnetic stirrer
Use ultrasonic disperse instrument to disperse after stirring, eventually pass and after filtering and being vacuum dried, be configured to composite powder；
C) composite powder made in step b) is placed in selective laser sintering system powder device, in argon protection
Under, selective sintering is carried out to powder.
The described ultrasonic disperse time is 30 minutes, and vacuum drying temperature is 40～50 DEG C, and drying time is little for 7～9
When.
Described selective laser sintering technological parameter is spot diameter 0.6～0.8mm, powdering thickness 0.1～0.15mm,
Laser power is 2.75w, scanning speed 500mm/min.
Advantage of the present invention is as follows:
1. utilize the feature that mesopore silicon oxide specific surface area is high, pore volume is big, improve the cell adhesion ability of support.
2. form cross-linked network as crosslinking points with polyglycolic acid by the use of mesopore silicon oxide granule, improve the intensity of support
And hardness.
3. utilize the element silicon contained by mesopore silicon oxide to promote collage synthesis and calcified bone matrix, promote the formation of new bone.
Specific embodiment
With reference to an embodiment, the specific embodiment of the present invention is further described, but the content of the present invention is simultaneously
It is not limited to this.
The preparation process of mesopore silicon oxide/polyglycolic acid support:
(1) weigh 5 grams of mesopore silicon oxides and 95 grams of polyglycolic acid powder carry out simple and mechanical mixing；
(2) load weighted for step (1) mixed-powder is added in 1000 milliliters of anhydrous alcohols, filled using magnetic stirrer
Carry out disperseing 30 minutes with ultrasonic disperse instrument again after dividing stirring, eventually pass filter and be vacuum dried 8 hours at 45 DEG C
It is configured to composite powder afterwards；
(3) composite powder preparing step (2) is placed in selective laser sintering system powder device, protects in argon
Under shield, selective sintering is carried out to powder, sintering process parameter is spot diameter 0.7mm, powdering thickness 0.12mm,
Laser power 2.75w, scanning speed 500mm/min.
Claims (3)
1. the present invention relates to a kind of cell adhesion performance improving polyglycolic acid bone support using mesopore silicon oxide, with
Its mechanical performance of Shi Tigao and the method for biological activity.Its preparation technology is:
(1) weigh 5% mesopore silicon oxide respectively by mass fraction and 95% polyglycolic acid powder carries out simple and mechanical mixing
Close；
(2) load weighted for step (1) mixed-powder is added in a certain amount of anhydrous alcohol, filled using magnetic stirrer
Disperseed with ultrasonic disperse instrument after dividing stirring, eventually pass and after filtering and being vacuum dried, be configured to composite powder；
(3) composite powder preparing is placed in selective laser sintering system powder device, under argon protection,
Selective sintering is carried out to powder.
2. according to the method described in claim 1 it is characterised in that: the described ultrasonic disperse time be 30 minutes,
Vacuum drying temperature is 40～50 DEG C, and drying time is 7～9 hours.
3. according to the method described in claim 1 it is characterised in that: described selective laser sintering technique ginseng
Number is spot diameter 0.6～0.8mm, powdering thickness 0.1～0.15mm, and laser power is 2.75w, scanning speed
500mm/min.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510408546.5A CN106344966A (en) | 2015-07-13 | 2015-07-13 | Method for improving cell adhesion of polyglycolic acid stent by using mesoporous silica |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510408546.5A CN106344966A (en) | 2015-07-13 | 2015-07-13 | Method for improving cell adhesion of polyglycolic acid stent by using mesoporous silica |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106344966A true CN106344966A (en) | 2017-01-25 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510408546.5A Pending CN106344966A (en) | 2015-07-13 | 2015-07-13 | Method for improving cell adhesion of polyglycolic acid stent by using mesoporous silica |
Country Status (1)
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108686271A (en) * | 2018-06-29 | 2018-10-23 | 中南大学 | A kind of preparation method with antibacterial functions Ag-DMBG/PLLA Composite Bone holders |
| CN108744029A (en) * | 2018-06-29 | 2018-11-06 | 中南大学 | A method of preparing PGA/DMBG Composite Bone holders using poly-dopamine modification mesoporous bioglass |
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| CN101721751A (en) * | 2008-10-10 | 2010-06-09 | 张阳德 | Human tissue engineering support loaded with controlled-release cell growth factor and provided with hollow silicon dioxide ball with kernel and preparation method and applications thereof |
| CN102051702A (en) * | 2010-12-03 | 2011-05-11 | 华东理工大学 | Mesoporous silicon oxide particle/degradable polymer nano composite fiber, preparation method and application thereof |
| CN102321269A (en) * | 2011-09-26 | 2012-01-18 | 浙江工业大学 | Method for preparing meso-porous silica/polylactic acid foamed material by supercritical CO2 foaming |
| CN103656737A (en) * | 2012-09-25 | 2014-03-26 | 中南大学 | Preparation method of HAP/TCP/TTCP (hydroxylapatite/tricalcium phosphate/tetracalcium) multiphase porous ceramic artificial bone |
| CN103709737A (en) * | 2013-12-05 | 2014-04-09 | 吴江中瑞机电科技有限公司 | High-molecular composite powder material for SLS and preparation method therefor |
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2015
- 2015-07-13 CN CN201510408546.5A patent/CN106344966A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101721751A (en) * | 2008-10-10 | 2010-06-09 | 张阳德 | Human tissue engineering support loaded with controlled-release cell growth factor and provided with hollow silicon dioxide ball with kernel and preparation method and applications thereof |
| CN102051702A (en) * | 2010-12-03 | 2011-05-11 | 华东理工大学 | Mesoporous silicon oxide particle/degradable polymer nano composite fiber, preparation method and application thereof |
| CN102321269A (en) * | 2011-09-26 | 2012-01-18 | 浙江工业大学 | Method for preparing meso-porous silica/polylactic acid foamed material by supercritical CO2 foaming |
| CN103656737A (en) * | 2012-09-25 | 2014-03-26 | 中南大学 | Preparation method of HAP/TCP/TTCP (hydroxylapatite/tricalcium phosphate/tetracalcium) multiphase porous ceramic artificial bone |
| CN103709737A (en) * | 2013-12-05 | 2014-04-09 | 吴江中瑞机电科技有限公司 | High-molecular composite powder material for SLS and preparation method therefor |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108686271A (en) * | 2018-06-29 | 2018-10-23 | 中南大学 | A kind of preparation method with antibacterial functions Ag-DMBG/PLLA Composite Bone holders |
| CN108744029A (en) * | 2018-06-29 | 2018-11-06 | 中南大学 | A method of preparing PGA/DMBG Composite Bone holders using poly-dopamine modification mesoporous bioglass |
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Application publication date: 20170125 |
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