CN106215232A - A kind of wound antibacterial promoting healing dressing and preparation method thereof - Google Patents
A kind of wound antibacterial promoting healing dressing and preparation method thereof Download PDFInfo
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- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0009—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form containing macromolecular materials
- A61L26/0028—Polypeptides; Proteins; Degradation products thereof
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- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0004—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form containing inorganic materials
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- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0009—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form containing macromolecular materials
- A61L26/0014—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form containing macromolecular materials obtained by reactions only involving carbon-to-carbon unsaturated bonds
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- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
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- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
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- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
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Abstract
The invention discloses a kind of wound antibacterial promoting healing dressing, its effective ingredient is to include the fibroin albumen raw material of mix homogeneously, bioactivity glass raw material, chitosan raw material and the paste of polyvinyl alcohol raw material.Also disclose the preparation method of this dressing, first by silk fibroin protein solution and bioactivity glass powder mix homogeneously, add chitosan solution and poly-vinyl alcohol solution stirs into mixed solution, thaw through multigelation and can be prepared by Hard agglut wound dressing materials, by adjusting raw material silk fibroin protein solution, chitosan solution, ratio between poly-vinyl alcohol solution and bioactivity glass, permeable gas performance, water absorption rate, water retention and the bacteriostasis property of the dressing obtained can be controlled so that it is meet the requirement promoting wound healing；And this preparation method has, and reaction condition is gentle, technique is simple, the advantage of low raw-material cost, is suitable to industrial amplification production.
Description
Technical field
The present invention relates to wound dressing preparation technique field, in particular to a kind of wound antibacterial promoting healing dressing and system thereof
Preparation Method.
Background technology
Along with living standard improves, Wound healing and bone regeneration receives publicity with nursing.For some Hard agglut wounds, common wrapping is controlled
Treatment is often difficult to healing, needs to use any special measures.Hard agglut wound generally comprises pressure ulcer, diabetic foot, arteriovenous ulcer, evil
Property the chronic difficult wound such as tumor, the surgical wound such as traffic accident injury, wound class acute wounds, burn shaping, cardiac stent operation, fat
The surgical wounds such as fat liquefaction, Abscess incision drain.Wound difficulty healing reason, has some systemic disease to cause human immunity thin
Born of the same parents are impaired；Old, malnutrition etc. causes cell regeneration scarce capacity；Traumatic infection, inflammatory reaction etc..It has its source in
Collage synthesis is bad, fibroblast metabolism is impaired.Partial clinical doctor is also being devoted to the treatment of Hard agglut wound, some doctors
Institute's wound dressing usage amount can reach annual more than more than 70 ten thousand times, and demand is very big.
The wound dressing used clinically at present mainly has traditional dressing and novel moist dressing.Traditional dressing i.e. gauze,
Iodoform gauzes etc., are dried with traditional holding wound surface and carry out dressing change, several effects without promoting healing for guideline.Novel moist
Dressing includes the cover type outer layer dressing with transparent film type dressing as representative；In with alginate dressing, wound glue as representative
Layer filling type dressing；Antibiotic property dressing etc. with silver ion dressing as representative, with wet union as principle, can create beneficially cell
Growing suitable moist environment, dressing is airtight or semi-hermetic wound can keep wound constant temperature, prevents antibacterial and extraneous graininess different
Thing invades, and reduces wound infection chance, more will not produce mechanical injuries, wound healing promoting during change dressings.But it is at moistening ring
The characteristic and the permeability that absorb sepage under border are poor, and final infection conditions occurs many, becomes a medically difficult problem.
In recent years, the third generation dressing of referred to as future technology type becomes exploitation focus.It is characterized in: there is intelligence and automatically adjust
Joint wound moist degree performance, allows tissue, cell be in optimal growth environment；The composition of dressing can be cell, tissue growth
Nutrition is provided, and there is suppression bacterial population.What the most representational i.e. D. funk etc. was invented has vacuum reservoir
Wound dressing, energy draining wound sepage in time, keep wound the most infected, wound face material tool trophic function, wound healing is fast,
But price is high, patient is difficult to bear.
Therefore, research and develop and a kind of have third generation dressing feature and to reduce the casting product of cost be the problem needing solution badly.
Summary of the invention
For above-mentioned the deficiencies in the prior art, the technical problem to be solved is: how to provide a kind of breathability
Good, water absorption rate, water retention and bacteriostasis property are superior, with low cost, it is possible to advantageously promote the antibacterial rush of wound of wound healing more
Close dressing and preparation method thereof so that it is be particularly well-suited to Hard agglut wound and use.
In order to solve above-mentioned technical problem, present invention employs following technical scheme:
A kind of wound antibacterial promoting healing dressing preparation method, it is characterised in that by silk fibroin protein solution and bioactivity glass powder
End mix homogeneously, adds chitosan solution and after poly-vinyl alcohol solution stirs into mixed solution, thaws through multigelation and obtain.
Wound antibacterial promoting healing dressing prepared by this method, owing to raw material have employed silk fibroin protein solution, bioactivity glass
Glass powder, and chitosan solution and four kinds of materials of poly-vinyl alcohol solution, fibroin albumen is the natural polymer extracted from cocoon shell
Sub-protein, containing 18 kinds of aminoacid, wherein glycine (Gly), alanine (Ala) and serine (Ser) account for always forming
80%.The aggregated structure of fibroin albumen includes crystallization and amorphous two large divisions.Fibroin has the property that both sexes are charged,
There is no toxicity, there is good biocompatibility and biodegradable, and the growth to epidermis cell has facilitation, can be
Wound tissue provides the nutrient substance such as aminoacid；Bioactivity glass has the table of uniqueness as lithotroph synthetic material
Face activity, the distinctive chemical composition of bioactivity glass, particularly its calcium, phosphorus plasma deposition generate a shelf-like hydroxy-apatite
Rock layers, thus make it have huge specific surface area, beneficially cell tactophily, beneficially nutrition and oxygen and enter, metabolite
Discharging, the most beneficially blood vessel and nerve is grown into, thus is promoted wound healing.Can also the epithelial growth of routinely inducing cell itself
The factor synthesizes, and provides the native epithelium somatomedin with complete biological function of patient self for wound surface local, fast to wound surface
Speed healing has played important function；Chitosan have pain relieving, stop blooding, promote wound healing, reduce cicatrix, antibacterial, good biology
The performance that the compatibility and biodegradability etc. are excellent, is very suitable as the raw material of wound dressing；Polyvinyl alcohol hydrogel
(Polyvinyl alcohol, PVA) is that one has three-dimensional cross-linked cancellated water-soluble polymer, has hydrophilic, soft
Soft, mildness and good biocompatibility, be widely used in biomedicine field.Utilize PVA when preparing repeatedly simultaneously
The characteristic that freeze thawing is thawed, when uniform PVA aqueous solution is cooled to be below the freezing point, macromolecular chain is squeezed ice (solid water)
Outside, occurs to be separated, and the local concentration of the strand in causing macromolecule mutually raises, and strand is mutually sufficiently close together and is formed
Hydrogen bond is down to the nuclei of crystallization.When freezing body and thawing, crystallization occurring further, the PVA micro-crystallization generated is constructed firmly
Three-dimensional net structure, is filled with water in this space.The PVA hydrogel water content that this method obtains is high, presents transparent on the whole.
As optimization, said method specifically includes following steps:
(1) silk fibroin powder is dissolved in deionized water, is made into the fibroin that quality concentration of volume percent is 2.0～5.0%
Protein solution；
Dissolve the chitosan in dilute acid soln, be made into the chitosan solution that quality concentration of volume percent is 1.5～2.5%；
Being dissolved in deionized water at 90 ~ 98 DEG C by polyvinyl alcohol, being made into quality concentration of volume percent is 5.0～10.0%
Poly-vinyl alcohol solution；
Obtain bioactivity glass powder；
(2) at room temperature, after silk fibroin protein solution step (1) obtained and bioactivity glass mix homogeneously, shell is added
Polysaccharide solution and poly-vinyl alcohol solution stir into mixed solution, and stir (about 30min), and wherein silk fibroin protein solution, shell gather
The volume ratio of sugar juice and poly-vinyl alcohol solution is 7 ~ 2:63 ~ 18:27 ~ 72；The ratio of bioactivity glass added is 0.5 ~
1.0% mass concentration of volume percent；Mixed solution is poured in mould, thaw again through repeatedly freezing, i.e. obtain dressing materials.
So, using above steps, fibroin albumen is soluble in deionized water, uses spirit of vinegar to dissolve chitosan, it is possible to
Chitosan is completely dissolved, and polyvinyl alcohol can only dissolve under high temperature bath.Therefore it is molten preferably to promote each raw material to complete
Solve mixing.
As optimization, in described step (2), mixing material freeze-thaw concrete operations are: first pour mixed solution into mould
In tool, in-20 DEG C of freezing 8 ~ 10h, then put at room temperature until thawing, the most repeatedly for three times.
Adopt and carry out freeze-thaw in this way, it is possible to make polyvinyl alcohol can occur to be separated and crystallization, generate
PVA micro-crystallization also constructs firm three-dimensional net structure.
As optimization, in described step (1), the preparation method of silk fibroin powder is: be dipped to by husks cocoon shell ethers
Few 48h, cleans with distilled water, is dried, to remove waxiness；Then soak at least 48h with alcohols, clean with distilled water, be dried, with
Remove partial organic substances and impurity；The husks cocoon shell mass percent 0.5% Na2CO3 solution of wash clean will be boiled about again
3h, removes sericin；Dry after the cocoon shell removing sericin is washed with deionized water only, be placed in volume ratio CaCl2:C2H5OH:H2O=
In the solution of 1:2:8, make fibroin be completely dissolved at water-bath 80 DEG C, obtain transparent silk fibroin solution, through dialysis, sucking filtration, concentration, cold
Lyophilizing is dry, obtains pure silk fibroin powder.
Silk fibroin powder uses said method to prepare, it is possible to increase the purity of fibroin albumen also reduces cost.
As optimization, in described step (1), bioactivity glass powder obtains according to following preparation method: will constitute biology
Each raw material mix homogeneously according to a certain percentage of activity glass, under room temperature, airtight stirring 1 hour, is at room temperature aged 3d, obtains
Uniformly colloidal sol, puts into uniform colloidal sol 1d in 75 DEG C of air dry ovens, obtains uniform wet gel, wet gel is put 150 DEG C of air blast
In drying baker, obtaining dry gel powder, then put into by xerogel in Muffle furnace, 700 DEG C maintain 2h, take out after cooling, obtain
Bioactivity glass powder.
In above-mentioned bioactivity glass powder method preparation method, existing bioactivity glass preparation method can be used
In raw material and ratio, it is also possible to preferably employ tetraethyl orthosilicate, triethyl phosphate, four water-calcium nitrate according to 12.5 mass parts:
8.5 mass parts: the raw material of 1.46 mass parts is prepared so that it is have synthesis temperature low, the feature that uniformity is good.It addition, knot
Closing the sol-gel process principle that preparation method uses, the bioactivity glass powder of preparation can have nanometer level microporous, huge
Big specific surface area, higher chemism and characterization of adsorption.
As optimizing further, in described step (1), diluted acid is spirit of vinegar, and concentration is 0.5 ~ 1.5%(V/V).The most permissible
Preferably dissolve chitosan, and spirit of vinegar is the most volatile.
As optimizing further, in described step (1), bioactivity glass powder is that after grinding, the particle diameter obtained that sieves is
The bioactivity glass powder of 75 ~ 100 m.As such, it is possible to preferably protect mixed effect, it is ensured that during use, medicinal ingredient fills
Distribution is waved.
The invention also discloses a kind of wound antibacterial promoting healing dressing, it is characterised in that its effective ingredient is for including mixing
Uniform fibroin albumen raw material, bioactivity glass raw material, chitosan raw material and the paste of polyvinyl alcohol raw material.
Wherein, described bioactivity glass raw material, fibroin albumen raw material, chitosan raw material and the quality of polyvinyl alcohol raw material
Number ratio is 0.5 ~ 1:1 ~ 2:2 ~ 9:8 ~ 3.Use said ratio, experiment proves that, so that medicinal effects reaches optimal.
Specifically use the dressing of this ratio, it is possible to making its permeable gas rate at 2132 ~ 2850g/ (m2 d), optimal permeable gas rate can
To reach 2460g/ (m2D), closest to the ideal value 2500 g/ (m of medical dressing2D), meanwhile, water absorption rate and water retention
23.70 g/g and 8.10 g/g are respectively reached, it is possible to absorb wound exudate and wound keeps moistening.
Further, the dressing of this wound antibacterial promoting healing uses aforesaid wound dressing preparation method to prepare.
Beneficial effects of the present invention is, the wound dressing of gained of the present invention has good biocidal property, it is to avoid wound infection.
The wound dressing of gained of the present invention has good permeable gas performance, water absorption and water-retaining property.The wound dressing of gained of the present invention
There is preferable promoting healing performance.Preparation method of the present invention has the advantage that.
In sum, the wound antibacterial promoting healing dressing that the present invention is obtained, there is good permeability, water absorption rate, water retention
Superior with bacteriostasis property, with low cost, it is possible to the advantage advantageously promoting wound healing, it is particularly well-suited to Hard agglut wound and makes
With.Its preparation method also has that reaction condition is gentle, technique simple, the advantage of low raw-material cost simultaneously.
Accompanying drawing explanation
Fig. 1 is macroscopical picture of the wound dressing materials of embodiment 5 preparation.
Fig. 2 is the XRD figure of the bioactivity glass of preparation.
Fig. 3 is the biocidal property collection of illustrative plates of the dressing lixiviating solution of the wound dressing of embodiment 5 preparation.
Fig. 4 is wound dressing, sodium alginate dressing, bioactivity glass powder and the hospital gauze group of embodiment 5 preparation
Each time point wound healing situation map postoperative to diabetes rat.
Fig. 5 be embodiment 5 preparation wound dressing, existing sodium alginate dressing, independent bioactivity glass powder and
The healing time figure of hospital gauze group correspondence wound surface.
Fig. 6 be embodiment 5 preparation wound dressing, existing sodium alginate dressing, independent bioactivity glass powder and
The Wound healing rate figure of phase point when hospital gauze group wound surface is each.
Detailed description of the invention
With detailed description of the invention effect of the present invention done checking below in conjunction with the accompanying drawings further.
Detailed description of the invention: a kind of wound antibacterial promoting healing dressing, prepares in accordance with the following methods:
(1) silk fibroin powder is dissolved in deionized water, is made into the fibroin that quality concentration of volume percent is 2.0～5.0%
Protein solution；
Dissolve the chitosan in dilute acid soln, be made into the chitosan solution that quality concentration of volume percent is 1.5～2.5%；
Being dissolved in deionized water at 90 ~ 98 DEG C by polyvinyl alcohol, being made into quality concentration of volume percent is 5.0～10.0%
Poly-vinyl alcohol solution；
Obtain bioactivity glass powder；
(2) at room temperature, after silk fibroin protein solution step (1) obtained and bioactivity glass mix homogeneously, shell is added
Polysaccharide solution and poly-vinyl alcohol solution stir into mixed solution, stir, wherein silk fibroin protein solution, chitosan solution and poly-
The volume ratio of glycohol solution is 7 ~ 2:63 ~ 18:27 ~ 72；The ratio of the bioactivity glass added is 0.5 ~ 1.0% mass body
Long-pending percent concentration；Mixed solution is poured in mould, thaw again through repeatedly freezing, i.e. obtain dressing materials.
Wherein, in described step (2), mixing material freeze-thaw concrete operations are: first pour mixed solution into mould
In, in-20 DEG C of freezing 8 ~ 10h, then put at room temperature until thawing, the most repeatedly for three times.
Wherein, in described step (1), the preparation method of silk fibroin powder is: soaked at least by husks cocoon shell ethers
48h, cleans with distilled water, is dried, to remove waxiness；Then soak at least 48h with alcohols, clean with distilled water, be dried, to remove
Remove partial organic substances and impurity；The husks cocoon shell mass percent 0.5% Na2CO3 solution of wash clean will boil about 3h again,
Remove sericin；The cocoon shell removing sericin is washed with deionized water clean after dry, be placed in volume ratio CaCl2:C2H5OH:H2O=1:2:
In the solution of 8, make fibroin be completely dissolved at water-bath 80 DEG C, obtain transparent silk fibroin solution, do through dialysis, sucking filtration, concentration, freezing
Dry, obtain pure silk fibroin powder.
Wherein, in described step (1), bioactivity glass powder obtains according to following preparation method: will constitute biological activity
Each raw material mix homogeneously according to a certain percentage of glass, under room temperature, airtight stirring 1 hour, is at room temperature aged 3d, obtains uniformly
Colloidal sol, puts into uniform colloidal sol 1d in 75 DEG C of air dry ovens, obtains uniform wet gel, and wet gel is put 150 DEG C of forced air dryings
In case, obtaining dry gel powder, then put into by xerogel in Muffle furnace, 700 DEG C maintain 2h, take out after cooling, obtain biology
Activity glass powder.Wherein, raw material prepared by bioactivity glass and ratio, use tetraethyl orthosilicate, triethyl phosphate, four water
Calcium nitrate is according to 12.5 mass parts: 8.5 mass parts: the raw material of 1.46 mass parts is prepared.
Wherein, in described step (1), diluted acid is spirit of vinegar, and concentration is 0.5 ~ 1.5%(V/V).
Wherein, in described step (1), bioactivity glass powder is that after grinding, the particle diameter obtained that sieves is 75 ~ 100 m's
Bioactivity glass powder.
Below in conjunction with choosing representative several embodiments and the accompanying drawing of different parameters, the present invention is carried out further
Concrete checking.
Embodiment 1, the wound antibacterial promoting healing dressing obtained in the present embodiment, its preparation method and above-mentioned embodiment system
Preparation Method is identical, and difference is that the parameter of each value range is chosen for: the quality volume basis of silk fibroin protein solution in step 1
Specific concentration is 5%；The quality concentration of volume percent of chitosan solution is 1.5%；The quality percent by volume of poly-vinyl alcohol solution
Concentration is 5%；In step 2, the volume ratio of silk fibroin protein solution, chitosan solution and poly-vinyl alcohol solution is 7:63:27；Add
The quality concentration of volume percent shared by bioactivity glass be 0.5%.
Embodiment 2, the wound antibacterial promoting healing dressing obtained in the present embodiment, its preparation method and above-mentioned embodiment system
Preparation Method is identical, and difference is that the parameter of each value range is chosen for: the quality volume basis of silk fibroin protein solution in step 1
Specific concentration is 3%；The quality concentration of volume percent of chitosan solution is 2%；The quality percent by volume of poly-vinyl alcohol solution is dense
Degree is 6%；In step 2, the volume ratio of silk fibroin protein solution, chitosan solution and poly-vinyl alcohol solution is 6:54:36.Add
Quality concentration of volume percent shared by bioactivity glass is 0.5%.
Embodiment 3, the wound antibacterial promoting healing dressing obtained in the present embodiment, its preparation method and above-mentioned embodiment system
Preparation Method is identical, and difference is that the parameter of each value range is chosen for: the quality volume basis of silk fibroin protein solution in step 1
Specific concentration is 4%；The quality concentration of volume percent of chitosan solution is 2.5%；The quality percent by volume of poly-vinyl alcohol solution
Concentration is 8%；In step 2, the volume ratio of silk fibroin protein solution, chitosan solution and poly-vinyl alcohol solution is 5:45:45.Add
The quality concentration of volume percent shared by bioactivity glass be 1%
Embodiment 4, the wound antibacterial promoting healing dressing obtained in the present embodiment, its preparation method and above-mentioned embodiment preparation side
Method is identical, and difference is that the parameter of each value range is chosen for: in step 1, the quality percent by volume of silk fibroin protein solution is dense
Degree is 2.5%；The quality concentration of volume percent of chitosan solution is 2%；The quality concentration of volume percent of poly-vinyl alcohol solution
It is 9%；In step 2, the volume ratio of silk fibroin protein solution, chitosan solution and poly-vinyl alcohol solution is 4:36:54.The life added
Quality concentration of volume percent shared by thing activity glass is 1%
Embodiment 5, the wound antibacterial promoting healing dressing obtained in the present embodiment, its preparation method and above-mentioned embodiment preparation side
Method is identical, and difference is that the parameter of each value range is chosen for: in step 1, the quality percent by volume of silk fibroin protein solution is dense
Degree is 2%；The quality concentration of volume percent of chitosan solution is 2%；The quality concentration of volume percent of poly-vinyl alcohol solution is
10%；In step 2, the volume ratio of silk fibroin protein solution, chitosan solution and poly-vinyl alcohol solution is 3:27:63.The biology added
Quality concentration of volume percent shared by activity glass is 0.5%
Embodiment 6, the wound antibacterial promoting healing dressing obtained in the present embodiment, its preparation method and above-mentioned embodiment preparation side
Method is identical, and difference is that the parameter of each value range is chosen for: in step 1, the quality percent by volume of silk fibroin protein solution is dense
Degree is 2%；The quality concentration of volume percent of chitosan solution is 1.5%；The quality concentration of volume percent of poly-vinyl alcohol solution
It is 10%；In step 2, the volume ratio of silk fibroin protein solution, chitosan solution and poly-vinyl alcohol solution is 2:18:72.The life added
Quality concentration of volume percent shared by thing activity glass is 1%
The wound antibacterial promoting healing dressing prepared the various embodiments described above below, carries out physics detection and physical property is examined
Survey.
Detecting through physics, in the dressing obtained by six groups of embodiments, bioactivity glass raw material, fibroin albumen are former
The mass fraction ratio of material, chitosan raw material and polyvinyl alcohol raw material each falls within the proportion of 0.5 ~ 1:1 ~ 2:2 ~ 9:8 ~ 3.
Table one is seen, it can be seen that the wound dressing that above-mentioned six embodiments are obtained through physical property testing result, its
Water absorption rate scope is 16.03 ~ 33.50g/g, and water retention scope is 6.01 ~ 11.40g/g, vapor pervious rate scope is 2132 ~
2850g/ (m2 d), therefore the wound antibacterial promoting healing dressing of each embodiment acquisition can reach good medicinal effects scope.
Wherein the permeable gas rate of embodiment 5 can reach 2460g/ (m2D), closest to ideal value 2500 g/ of medical dressing
(m2D), meanwhile, water absorption rate and water retention have respectively reached 23.70 g/g and 8.10 g/g, it is possible to absorb wound exudate and hinder
Mouth keeps moistening；Therefore embodiment 5 dressing that to be effect best.
Table 1
| Embodiment | Water absorption rate (g/g) | Water retention (g/g) | Vapor pervious rate g/(m2D) |
| 1 | 30.45 | 10.15 | 2205 |
| 2 | 28.14 | 9.03 | 2300 |
| 3 | 16.03 | 6.01 | 2850 |
| 4 | 19.52 | 7.32 | 2611 |
| 5 | 23.70 | 8.10 | 2460 |
| 6 | 33.50 | 11.40 | 2132 |
Testing inspection checking is used to implement the medicinal effects of 5 wound dressings prepared further below.
Fig. 1 is the dressing macro morphology figure of embodiment 5 preparation, it can be seen that its properties of transparency and aqueous performance, and can
Find out that there is preferable processability.
Fig. 2 is the intermediate product prepared in above-described embodiment: the XRD figure of bioactivity glass, it can be seen that biological activity
Glass presents broad, disperse, sharp-pointed diffraction maximum, and explanation is amorphous state or amorphous state inorganic solid particles.Wherein Si-O-
The bond angle of Si, P-O-P, Si-O-P key changes the most within the specific limits, does not have fixing bond angle and spacing of lattice so that amorphous
State material has the structure of shortrange order, longrange disorder, and they are with amorphous state as principal character.
Fig. 3 is that the lixiviating solution of the dressing of embodiment 5 carries out the biocidal property collection of illustrative plates that bacteriostasis property research obtains, and therefrom can see
Going out dressing lixiviating solution and escherichia coli, staphylococcus aureus, bacillus pyocyaneus are respectively provided with stronger biocidal property, material lixiviating solution is dense
Spending the biggest, biocidal property is the strongest.On the one hand the amino (NH2+) being possibly due in chitosan solution, can be combined with bacteria cell wall
Form negative electricity environment, destroy the integrity of bacteria cell wall, and then destroy antibacterial until death；On the other hand it is possibly due to silk
Some amino of fibroin can adsorb antibacterial, the normal activities of interference antibacterial, produces corresponding biocidal property；Also has a side
Face is probably the bioactivity glass inhibitory action to antibacterial, has document to show, bioactivity glass can be partially formed alkali
Property environment, can play certain bacteriostasis.To sum up stating analysis, material lixiviating solution is higher than golden yellow to colibacillary biocidal property
Staphylococcus and bacillus pyocyaneus, this may be relevant with the structure of antibacterial and drug resistance, and escherichia coli belong to gram negative bacteria, carefully
Cell wall is two-dimensional structure and relatively thin；Staphylococcus aureus belongs to gram positive bacteria, and cell wall is three dimensional structure and thicker；Green
Pus bacillus belongs to pseudomonas, is the gram negative bacteria that the most common drug resistance is stronger.
Fig. 4 is that the dressing of embodiment 5, sodium alginate dressing, bioactivity glass powder and hospital gauze group are to diabetes
The postoperative each time point wound healing situation map of rat, by the perusal of wound surface it can be seen that during 2d, sodium alginate dressing group
Wound has part granulation tissue to be formed, and Hard agglut wound dressing group wound surface has one layer of whiteness to generate.During 4d, difficulty is more
Closing wound dressing group wound surface to reduce, other two groups all there is not significant change.During 8d, Hard agglut wound dressing group wound surface substantially subtracts
Little, granulation tissue is full of wound surface, and wound surface color is thin out, and sodium alginate group also forms granulation tissue, and wound surface has reduced, medical yarn
Cloth group has part granulation tissue to generate.During 10d, Hard agglut wound dressing group new life epithelial tissue major part flap coverage, sea
Sodium alginate group wound surface is obviously reduced, and hospital gauze group wound surface reduces inconspicuous.During 12d, Hard agglut wound dressing group new life epithelium
Tissue has been completely covered wound surface, and wound heals completely, and sodium alginate group wound surface reduces but heals the most completely, and hospital gauze group has meat
Bud organizes the formation of, and wound surface has certain reduction.
Fig. 5 is that the dressing of embodiment 5, sodium alginate dressing, bioactivity glass powder and hospital gauze group are to diabetes
Rat healing postoperative wound surface time diagram, from wound healing time (Fig. 5) it can be seen that difficult healing dressings group the average healing is
(11.2 ± 0.5) d, sodium alginate dressing group is (16.1 ± 0.67) d, and hospital gauze group is (20.5 ± 1.2) d, and difficult healing
Dressing group has significant difference (p < 0.05), difficult healing dressings group healing time to be significantly shorter than alginic acid compared with hospital gauze group
Sodium dressing group (p < 0.05).
Fig. 6 is that the dressing of embodiment 5, sodium alginate dressing, bioactivity glass powder and hospital gauze group are to diabetes
Rat healing postoperative wound surface rate figure, time same, the healing rate of phase point difficulty healing dressings group is apparently higher than sodium alginate dressing group, life
Thing activity glass group and hospital gauze group (p < 0.05), healing is very fast.
Claims (10)
1. a wound antibacterial promoting healing dressing preparation method, it is characterised in that by silk fibroin protein solution and bioactivity glass
Powder mix homogeneously, adds chitosan solution and after poly-vinyl alcohol solution stirs into mixed solution, thaws through multigelation
Arrive.
2. wound antibacterial promoting healing dressing preparation method as claimed in claim 1, it is characterised in that specifically include following step
Rapid:
(1) silk fibroin powder is dissolved in deionized water, is made into the sericin that quality concentration of volume percent is 2.0～5.0%
Protein solution；
Dissolve the chitosan in dilute acid soln, be made into the chitosan solution that quality concentration of volume percent is 1.5～2.5%；
Being dissolved in deionized water at 90 ~ 98 DEG C by polyvinyl alcohol, being made into quality concentration of volume percent is 5.0～10.0%
Poly-vinyl alcohol solution；
Obtain bioactivity glass powder；
(2) at room temperature, after silk fibroin protein solution step (1) obtained and bioactivity glass mix homogeneously, shell is added
Polysaccharide solution and poly-vinyl alcohol solution stir into mixed solution, stir, wherein silk fibroin protein solution, chitosan solution and poly-
The volume ratio of glycohol solution is 7 ~ 2:63 ~ 18:27 ~ 72；The ratio of the bioactivity glass added is 0.5 ~ 1.0% mass body
Long-pending percent concentration；Mixed solution is poured in mould, thaw again through repeatedly freezing, i.e. obtain dressing materials.
Wound the most according to claim 2 antibacterial promoting healing dressing preparation method, it is characterised in that in described step (2)
Mixing material freeze-thaw concrete operations are: first pour in mould by mixed solution, in-20 DEG C of freezing 8 ~ 10h, then be placed in room
Temperature is lower until thawing, the most repeatedly for three times.
Wound the most according to claim 2 antibacterial promoting healing dressing preparation method, it is characterised in that in described step (1)
The preparation method of silk fibroin powder is: husks cocoon shell ethers is soaked at least 48h, cleans with distilled water, is dried, to remove
Waxiness；Then soak at least 48h with alcohols, clean with distilled water, be dried, to remove partial organic substances and impurity；To wash dry again
Clean husks cocoon shell mass percent 0.5% Na2CO3 solution boils about 3h, removes sericin；The cocoon shell removing sericin is used
Deionized water dries after cleaning, and is placed in the solution of volume ratio CaCl2:C2H5OH:H2O=1:2:8, makes fibroin at water-bath 80 DEG C
It is completely dissolved, obtains transparent silk fibroin solution, through dialysis, sucking filtration, concentration, lyophilization, obtain pure silk fibroin powder.
Wound the most according to claim 2 antibacterial promoting healing dressing preparation method, it is characterised in that in described step (1)
Bioactivity glass powder obtains according to following preparation method: mixed according to a certain percentage by each raw material constituting bioactivity glass
Closing uniformly, under room temperature, airtight stirring 1 hour, is at room temperature aged 3d, obtains uniform colloidal sol, and uniform colloidal sol is put into 75 DEG C of air blast
1d in drying baker, obtains uniform wet gel, is put by wet gel in 150 DEG C of air dry ovens, obtains dry gel powder, then will be dry solidifying
Glue is put in Muffle furnace, and 700 DEG C maintain 2h, take out after cooling, obtain bioactivity glass powder.
Wound the most according to claim 2 antibacterial promoting healing dressing preparation method, it is characterised in that in described step (1)
Diluted acid is spirit of vinegar, and concentration is 0.5 ~ 1.5%(V/V).
Wound the most according to claim 2 antibacterial promoting healing dressing preparation method, it is characterised in that in described step (1)
Bioactivity glass powder be grind after sieve the bioactivity glass powder that particle diameter is 75 ~ 100 m obtained.
8. a wound antibacterial promoting healing dressing, it is characterised in that its effective ingredient is to include that the fibroin albumen of mix homogeneously is former
Material, bioactivity glass raw material, chitosan raw material and the paste of polyvinyl alcohol raw material.
9. wound antibacterial promoting healing dressing as claimed in claim 8, it is characterised in that described bioactivity glass raw material, silk
The mass fraction ratio of fibroin raw material, chitosan raw material and polyvinyl alcohol raw material is 0.5 ~ 1:1 ~ 2:2 ~ 9:8 ~ 3.
10. wound antibacterial promoting healing dressing as claimed in claim 8, it is characterised in that have employed in claim 1-7 arbitrary
Item preparation method described in claim prepares.
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