CN106215209A - A kind of the sterilization effect method of inspection, Apparatus and system - Google Patents
A kind of the sterilization effect method of inspection, Apparatus and system Download PDFInfo
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- CN106215209A CN106215209A CN201610608239.6A CN201610608239A CN106215209A CN 106215209 A CN106215209 A CN 106215209A CN 201610608239 A CN201610608239 A CN 201610608239A CN 106215209 A CN106215209 A CN 106215209A
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- 230000001954 sterilising effect Effects 0.000 title claims abstract description 83
- 238000004659 sterilization and disinfection Methods 0.000 title claims abstract description 81
- 238000000034 method Methods 0.000 title claims abstract description 34
- 230000000694 effects Effects 0.000 title claims abstract description 20
- 238000007689 inspection Methods 0.000 title abstract description 13
- 230000003287 optical effect Effects 0.000 claims abstract description 56
- 230000005284 excitation Effects 0.000 claims abstract description 51
- 239000000090 biomarker Substances 0.000 claims abstract description 37
- 238000012544 monitoring process Methods 0.000 claims description 29
- 238000012360 testing method Methods 0.000 claims description 17
- 238000001514 detection method Methods 0.000 claims description 16
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 claims description 12
- 229910052710 silicon Inorganic materials 0.000 claims description 12
- 239000010703 silicon Substances 0.000 claims description 12
- 238000012545 processing Methods 0.000 claims description 9
- 238000001914 filtration Methods 0.000 claims description 6
- 239000004973 liquid crystal related substance Substances 0.000 claims description 6
- 238000012258 culturing Methods 0.000 claims description 4
- 238000006243 chemical reaction Methods 0.000 claims description 3
- 238000012795 verification Methods 0.000 claims 1
- 210000004215 spore Anatomy 0.000 description 23
- 230000008569 process Effects 0.000 description 16
- 239000000126 substance Substances 0.000 description 9
- 241000894006 Bacteria Species 0.000 description 7
- 244000005700 microbiome Species 0.000 description 6
- 102000004190 Enzymes Human genes 0.000 description 5
- 108090000790 Enzymes Proteins 0.000 description 5
- 239000003708 ampul Substances 0.000 description 4
- 230000001580 bacterial effect Effects 0.000 description 4
- 210000004666 bacterial spore Anatomy 0.000 description 4
- 239000002696 acid base indicator Substances 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 230000035784 germination Effects 0.000 description 3
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 238000004590 computer program Methods 0.000 description 2
- 230000006870 function Effects 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- 230000006698 induction Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000004083 survival effect Effects 0.000 description 2
- IAYPIBMASNFSPL-UHFFFAOYSA-N Ethylene oxide Chemical compound C1CO1 IAYPIBMASNFSPL-UHFFFAOYSA-N 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 238000009629 microbiological culture Methods 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 238000012414 sterilization procedure Methods 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/26—Accessories or devices or components used for biocidal treatment
- A61L2/28—Devices for testing the effectiveness or completeness of sterilisation, e.g. indicators which change colour
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2202/00—Aspects relating to methods or apparatus for disinfecting or sterilising materials or objects
- A61L2202/20—Targets to be treated
- A61L2202/24—Medical instruments, e.g. endoscopes, catheters, sharps
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- Health & Medical Sciences (AREA)
- Epidemiology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The embodiment of the invention discloses a kind of the sterilization effect method of inspection, Apparatus and system.The method includes: when inspection enabling signal being detected, send control instruction to excitation source, so that excitation source sends corresponding exciting light according to control instruction；Obtaining the light signal acquisition device signal of telecommunication according to the optical signal output collected, optical signal is that the biological indicator after sterilization treatment produces when the light that is excited irradiates；The magnitude of voltage of the signal of telecommunication is monitored in real time, and judges whether sterilizing success according to magnitude of voltage.The embodiment of the present invention by being converted into the signal of telecommunication by the optical signal that biological indicator produces, and monitors the magnitude of voltage of the signal of telecommunication, to judge sterilizing whether success, compared with prior art, has advantage short, easy and simple to handle Check-Out Time.
Description
Technical Field
The embodiment of the invention relates to a microorganism inspection device, in particular to a sterilization effect inspection method, a device and a system.
Background
The sterilization effect detection generally refers to the monitoring of the treatment result of sterilization of all bacteria and other organic living bodies on the surface of medical equipment and surgical instruments during the use process. The traditional sterilization process comprises high-temperature steam sterilization, ethylene oxide sterilization, hydrogen peroxide low-temperature plasma sterilization, radiation sterilization and the like.
In order to verify the effectiveness of the sterilization process, it is often necessary to test the sterilization results. Currently, biological indicators are widely used to test the effectiveness of sterilization processes, which basically operate to perform a complete sterilization procedure on a biological indicator containing spores of a microorganism and a medical instrument to be sterilized, then place the sterilized indicator in an environment suitable for germination and growth of the spores, and detect the survival rate of the spores.
The most traditional detection method and apparatus is to observe the survival status of spores by using a microscope, specifically: the sterilized spores were cultured at a specific temperature in a culture medium for about 72 hours, and the culture medium was observed under a microscope to determine whether or not any microorganism survived.
Later, a detection method and a detection device for indicating the pH value change in the bacterial growth process by using an acid-base indicator are developed, and specifically, the method comprises the following steps: the biological indicator consists of a bacterial sheet carrying bacterial spores and an ampoule bottle filled with a culture solution containing an acid-base indicator, and after sterilization is finished, the ampoule bottle is crushed to ensure that the bacterial sheet is fully contacted with the culture solution. Depending on whether the sterilization is successful, the following two situations can occur: if the sterilization fails and spores are not completely killed, bacteria grow after the spores are contacted with the culture solution, acidic or alkaline substances are discharged during the growth of the bacteria, and the substances change the pH value of the culture solution, so that the acid-base indicator contained in the culture solution changes color. If the sterilization is successful and all the bacterial spores die, no bacteria grow after the bacterial spores are contacted with the culture solution, and the pH value of the culture solution is not changed, so that the color of the culture solution is not changed, and the original color is still kept, thereby judging the success of the sterilization.
In the process of implementing the embodiment of the invention, the inventor finds that the two conventional methods have the problems of overlong inspection time and complex operation.
Disclosure of Invention
An object of the embodiments of the present invention is to solve the problems of long inspection time and complicated operation in the prior art.
The embodiment of the invention provides a sterilization effect inspection method, which comprises the following steps:
when a detection starting signal is detected, sending a control instruction to an excitation light source so that the excitation light source sends out corresponding excitation light according to the control instruction;
acquiring an electric signal output by an optical signal acquisition device according to the acquired optical signal, wherein the optical signal is generated when the biological indicator subjected to the sterilization treatment is irradiated by the exciting light;
and monitoring the voltage value of the electric signal in real time, and judging whether the sterilization is successful according to the voltage value.
Preferably, the step of judging whether the sterilization is successful according to the voltage value specifically includes:
if the voltage value is larger than a set voltage threshold value and the duration time is larger than a set time threshold value within the preset time, judging that the sterilization is successful;
if not, sterilization fails.
Preferably, the method further comprises:
acquiring an externally input threshold value adjusting instruction;
and adjusting the set voltage threshold or the set time threshold according to the threshold adjusting instruction.
The invention also provides a sterilization effect testing device, which comprises:
the instruction sending module is used for sending a control instruction to an excitation light source when detecting a detection starting signal so that the excitation light source can send out excitation light according to the control instruction;
the acquisition module is used for acquiring an electric signal output by the optical signal acquisition device according to the acquired optical signal, wherein the optical signal is generated when the biological indicator subjected to the sterilization treatment is irradiated by the exciting light;
and the monitoring processing module is used for monitoring the voltage value of the electric signal in real time and judging whether the sterilization is successful or not according to the voltage value.
The invention also provides a sterilization effect inspection system, which comprises:
the spore culture tank is used for culturing the biological indicator after the sterilization treatment;
the optical signal acquisition device is used for acquiring optical signals generated when the biological indicator is irradiated by exciting light and converting the acquired optical signals into electric signals;
the main processor is used for sending a control instruction to the excitation light source when detecting the detection starting signal so as to enable the excitation light source to send excitation light to the biological indicator; and monitoring the voltage value of the received electric signal in real time to judge whether the sterilization is successful according to the voltage value.
Preferably, the optical signal acquisition device includes: the first filter, the second filter, the silicon photodiode and the integrator;
the first filter is positioned between the excitation light source and the spore culture tank and is used for filtering the excitation light emitted by the excitation light source;
the second filter is positioned between the spore culture tank and the silicon photodiode and is used for filtering an optical signal generated by the biological indicator;
the silicon photodiode is used for converting the received optical signal into an electric signal and transmitting the electric signal to the integrator;
and the integrator is used for amplifying the electric signal.
Preferably, the method further comprises the following steps: a first slave processor;
the first slave processor is used for carrying out analog-to-digital conversion processing on the received electric signals; and collecting the temperature of the spore culture tank, and controlling a heater according to the collected temperature so as to adjust the temperature of the spore culture tank.
Preferably, the method further comprises the following steps: a second slave processor;
the second slave processor is respectively connected with the master singlechip, the liquid crystal display device and the button and is used for receiving monitoring data sent by the master processor through a USART (Universal asynchronous receiver transmitter) and forwarding the monitoring data to the liquid crystal display device for display; and forwarding an operation instruction input by a user through the button to the main processor through a USART transmitter so as to enable the main processor to execute corresponding operation.
Preferably, the method further comprises the following steps: an EEPROM memory;
the EEPROM memory is used for storing monitoring data, and the monitoring data is transmitted to the EEPROM memory by the main processor through the SPI interface.
Preferably, the master processor, the first slave processor and the second slave processor are all single-chip microcomputers.
According to the technical scheme, the sterilization effect testing method, the device and the system provided by the embodiment of the invention convert the optical signal generated by the biological indicator into the electric signal, and monitor the voltage value of the electric signal to judge whether sterilization is successful.
Drawings
The features and advantages of the present invention will be more clearly understood by reference to the accompanying drawings, which are illustrative and not to be construed as limiting the invention in any way, and in which:
fig. 1 is a schematic flow chart illustrating a sterilization effect testing method according to an embodiment of the present invention;
fig. 2 is a schematic structural diagram of a sterilization effect testing apparatus according to an embodiment of the present invention;
fig. 3 is a schematic structural diagram of a sterilization effect testing system according to an embodiment of the present invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are some, but not all, embodiments of the present invention. All other embodiments, which can be obtained by a person skilled in the art without any inventive step based on the embodiments of the present invention, are within the scope of the present invention.
Fig. 1 is a schematic flow chart of a sterilization effect testing method according to an embodiment of the present invention, and referring to fig. 1, the sterilization effect testing method includes:
110. when a detection starting signal is detected, the processor sends a control instruction to the excitation light source so that the excitation light source sends out corresponding excitation light according to the control instruction;
the processor generates a corresponding control instruction when receiving a detection start signal input from the outside, and sends the control instruction to the laser light source to trigger the laser light source to emit corresponding excitation light according to the control instruction;
it is understood that after the sterilization process is complete, the user places the biological indicator into the spore growth tank; the inspection starting signal is a signal for starting inspection, which is input by a user through a button, a switch and the like;
wherein the corresponding excitation light is excitation light of a wavelength associated with the bacteria to be detected.
120. Acquiring an electric signal output by an optical signal acquisition device according to the acquired optical signal, wherein the optical signal is generated when the biological indicator subjected to the sterilization treatment is irradiated by the exciting light;
the biological indicator is excited to generate fluorescence after the excitation light source emits excitation light to the biological indicator, the fluorescence is collected by the optical signal collecting device in the form of optical signals, and the optical signal collecting device converts the collected optical signals into electric signals and transmits the electric signals to the processor;
130. and monitoring the voltage value of the electric signal in real time, and judging whether the sterilization is successful according to the voltage value.
It should be noted that, the voltage values at different positions of the electric signal can be obtained according to the waveform of the electric signal, and the voltage values are monitored to determine whether the voltage values meet the sterilization requirements.
Therefore, the embodiment converts the optical signal generated by the biological indicator into the electric signal and monitors the voltage value of the electric signal to judge whether the sterilization is successful.
In this embodiment, step 130 specifically includes:
if the voltage value is larger than a set voltage threshold value and the duration time is larger than a set time threshold value within the preset time, judging that the sterilization is successful;
if not, sterilization fails.
It should be noted that, in the present embodiment, the detection of the inspection start signal is used as the start time, the length of the preset time is used as the range to monitor the voltage value, and if the voltage value is not monitored to be greater than the set voltage threshold within the preset time, it is determined that the sterilization fails;
if the electric signal with the voltage value larger than the set voltage threshold value is monitored in the preset time, further monitoring whether the duration time is larger than the set time threshold value, and if not, judging that the sterilization fails; if yes, the sterilization is judged to be successful.
The preset time, the set voltage threshold and the set time threshold are all adjustable.
In order to improve the applicability of the scheme, the scheme further comprises the following steps:
acquiring an externally input threshold value adjusting instruction;
and adjusting the set voltage threshold or the set time threshold according to the threshold adjusting instruction.
It should be noted that, since the characteristics of bacteria are different, the threshold values to be set may be different; therefore, the threshold is set to be adjustable in this embodiment, specifically: the user can write the adjusted command into the processor through the external device, so that the processor executes the adjusted command, and the threshold size is adjusted.
Method embodiments are described as a series of acts or combinations for simplicity of explanation, but it should be understood by those skilled in the art that the present invention is not limited by the order of acts or acts described, as some steps may occur in other orders or concurrently with other steps in accordance with the embodiments of the invention. Furthermore, those skilled in the art will appreciate that the embodiments described in the specification are presently preferred and that no particular act is required to implement the invention.
Fig. 2 is a schematic structural view of a sterilization effect testing apparatus according to an embodiment of the present invention, and referring to fig. 2, the sterilization effect testing apparatus includes: the monitoring system comprises an instruction sending module 21, an acquisition module 22 and a monitoring processing module 23; wherein,
the instruction sending module 21 is configured to send a control instruction to an excitation light source when detecting a detection start signal, so that the excitation light source sends out excitation light according to the control instruction;
the acquisition module 22 is configured to acquire an electrical signal output by the optical signal acquisition device according to the acquired optical signal, where the optical signal is generated when the biological indicator subjected to the sterilization treatment is irradiated by the excitation light;
and the monitoring processing module 23 is configured to monitor the voltage value of the electrical signal in real time, and determine whether sterilization is successful according to the voltage value.
It should be noted that, when detecting that the detection start signal is detected, the instruction sending module 21 sends a control instruction to the excitation light source, so that the excitation light source sends out excitation light according to the control instruction; at this time, the optical signal collecting device collects the optical signal generated when the biological indicator after the sterilization treatment is irradiated by the excitation light, converts the optical signal into an electrical signal and transmits the electrical signal to the obtaining module 22; the acquisition module 22 transmits the electrical signal to the monitoring processing module 23, the monitoring processing module 23 monitors the voltage value of the electrical signal in real time, and if the voltage value is larger than the set voltage threshold and the duration is larger than the set time threshold, it is determined that the sterilization is successful.
In order to improve the applicability of the scheme, the device further comprises: an instruction adjustment module;
and the instruction adjusting module is used for acquiring an externally input threshold adjusting instruction and adjusting the set voltage threshold or the set time threshold according to the threshold adjusting instruction.
As for the apparatus embodiment, since it is basically similar to the method embodiment, the description is relatively simple, and for the relevant points, reference may be made to the partial description of the method embodiment.
It should be noted that, in the respective components of the apparatus of the present invention, the components therein are logically divided according to the functions to be implemented thereof, but the present invention is not limited thereto, and the respective components may be newly divided or combined as necessary.
Fig. 3 is a schematic structural diagram of a sterilization effect testing system according to an embodiment of the present invention, and referring to fig. 3, the sterilization effect testing system includes: a spore culture tank 31, an optical signal acquisition device 32 and a main processor 33; wherein,
a spore culturing tank 31 for culturing the biological indicator after the sterilization treatment;
the optical signal acquisition device 32 is used for acquiring an optical signal generated when the biological indicator is irradiated by exciting light and converting the acquired optical signal into an electrical signal;
a main processor 33, configured to send a control instruction to an excitation light source when detecting a test start signal, so that the excitation light source emits excitation light to the biological indicator; and monitoring the voltage value of the received electric signal in real time to judge whether the sterilization is successful according to the voltage value.
The biological indicator after sterilization treatment is inserted into a spore culture tank 31, and is cultured at a certain temperature, the bacterial spores which are not killed germinate and grow in a culture solution contained in the biological indicator, an active enzyme is generated in the growth and propagation process and reacts with substances in the culture solution to generate fluorescent substances, excitation light emitted by an excitation light source irradiates the fluorescent substances to excite the fluorescent substances to generate fluorescence, the fluorescence is collected by an optical signal collection device 32 and is converted into an electric signal and then is transmitted to a main processor 33, the main processor 33 processes and monitors the collected signal, when the signal value is continuously greater than a set threshold value, sterilization failure is determined, otherwise, sterilization success is determined; the main processor 33 is preferably a single chip microcomputer.
In this embodiment, the optical signal acquisition device 32 includes: the first filter, the second filter, the silicon photodiode and the integrator;
the first filter is positioned between the excitation light source and the spore culture tank and is used for filtering the excitation light emitted by the excitation light source;
the second filter is positioned between the spore culture tank and the silicon photodiode and is used for filtering an optical signal generated by the biological indicator;
the silicon photodiode is used for converting the received optical signal into an electric signal and transmitting the electric signal to the integrator;
and the integrator is used for amplifying the electric signal.
It should be noted that, the optical signal acquisition device 32 filters the acquired optical signal through the second optical filter, converts the optical signal into a weak electrical signal through the silicon photodiode, amplifies the electrical signal through the integrator, converts the amplified electrical signal into an output voltage of 0 to 5 volts, and sends the output voltage to the main single chip microcomputer through the IIC bus, and the main single chip microcomputer processes and monitors the acquired optical signal.
In another possible embodiment, the apparatus further comprises: a first slave processor;
the first slave processor is used for carrying out analog-to-digital conversion processing on the received electric signals; collecting the temperature of the spore culture tank, and controlling a heater according to the collected temperature to adjust the temperature of the spore culture tank; the first slave processor is preferably a single chip microcomputer, namely the first slave single chip microcomputer in fig. 3.
Referring to fig. 3, after the integrator amplifies the electrical signal, the first slave single chip sends the output electrical signal to the master single chip through the IIC bus after the ADC processes the output electrical signal;
moreover, the first slave single chip microcomputer also has the function of controlling the temperature of the culture tank, and the implementation process is as follows: acquiring temperature information of a culture tank acquired by a temperature sensor in real time, comparing the current temperature with a preset temperature to generate a control instruction, and sending the control instruction to a temperature induction control system to enable the temperature induction control system to execute heating action;
the temperature sensing control system consists of a third slave processor, a thermocouple and a heating belt, and the temperature range of the temperature sensing control system is controlled to be 45-70 ℃.
In another possible embodiment, the apparatus further comprises: a second slave processor;
the second slave processor is respectively connected with the master singlechip, the liquid crystal display device and the button and is used for receiving monitoring data sent by the master processor through a USART (Universal asynchronous receiver transmitter) and forwarding the monitoring data to the liquid crystal display device for display; and forwarding an operation instruction input by the user through the button to the main processor through the USART transmitter so that the main processor executes a corresponding operation, wherein the second processor is preferably a single chip microcomputer, namely a second slave single chip microcomputer in fig. 3.
In another possible embodiment, the apparatus further comprises: an EEPROM memory;
the EEPROM memory is used for storing monitoring data, and the monitoring data is transmitted to the EEPROM memory by the main processor through the SPI interface.
It should be noted that, in the present embodiment, the existing information is erased and reprogrammed by the EEPROM memory, so as to improve the operability of the system.
The operation of the system is illustrated by way of example as follows:
commercially available biological indicators are placed in the sterilizer along with the instruments to be sterilized to perform the sterilization process, wherein the biological indicators are placed in the sterilizer in the areas where sterilization is least effective (the specific location of the area may be consulted with the sterilizer manufacturer). After the sterilization process is finished, placing the biological indicator for the sterilization process in the culture tank, setting the temperature of the culture tank to be 56 ℃, breaking an ampoule bottle of the biological indicator by squeezing, enabling a culture solution in the ampoule bottle to be in contact with spores, carrying out microbial culture on the non-killed spores in the environment of the culture solution and at a set temperature, generating special enzyme in the germination and culture processes of the spores, and reacting the enzyme with an indicator added in the culture solution to generate a substance capable of generating fluorescence; starting microorganism culture and starting an ultraviolet light source (excitation light source), wherein the light source irradiates a biological indicator through a first optical filter, and almost no fluorescent substance exists in the initial stage of microorganism germination, so that a silicon photodiode cannot receive a fluorescent signal, the amount of generated active enzyme is increased along with the growth of microorganisms, the enzyme decomposes an indicator in a culture solution to generate a large amount of fluorescent substance to be increased, the light signal received by the silicon photodiode is enhanced, the light signal is amplified by an integrator to output a voltage signal, the voltage signal is received and processed by a singlechip, when the voltage signal is continuously greater than a set value, the invention outputs a positive signal to indicate sterilization failure, and if the voltage signal is not detected to exceed the set value within 1 hour, the sterilization is judged to be successful.
Therefore, the scheme can more sensitively discover the growth of bacteria in the biological indicator when the sterilization fails, the time for detecting and discovering bacterial colonies is short, the detection can be completed within 1 hour, and the method is very convenient and fast.
Various component embodiments of the invention may be implemented in hardware, or in software modules running on one or more processors, or in a combination thereof. In the device, the PC remotely controls the equipment or the device through the Internet, and accurately controls each operation step of the equipment or the device. The present invention may also be embodied as apparatus or device programs (e.g., computer programs and computer program products) for performing a portion or all of the methods described herein. The program for realizing the invention can be stored on a computer readable medium, and the file or document generated by the program has statistics, generates a data report and a cpk report, and the like, and can carry out batch test and statistics on the power amplifier. It should be noted that the above-mentioned embodiments illustrate rather than limit the invention, and that those skilled in the art will be able to design alternative embodiments without departing from the scope of the appended claims. In the claims, any reference signs placed between parentheses shall not be construed as limiting the claim. The word "comprising" does not exclude the presence of elements or steps not listed in a claim. The word "a" or "an" preceding an element does not exclude the presence of a plurality of such elements. The invention may be implemented by means of hardware comprising several distinct elements, and by means of a suitably programmed computer. In the unit claims enumerating several means, several of these means may be embodied by one and the same item of hardware. The usage of the words first, second and third, etcetera do not indicate any ordering. These words may be interpreted as names.
Although the embodiments of the present invention have been described in conjunction with the accompanying drawings, those skilled in the art may make various modifications and variations without departing from the spirit and scope of the invention, and such modifications and variations fall within the scope defined by the appended claims.
Claims (10)
1. A sterilization effect testing method is characterized by comprising the following steps:
when a detection starting signal is detected, sending a control instruction to an excitation light source so that the excitation light source sends out corresponding excitation light according to the control instruction;
acquiring an electric signal output by an optical signal acquisition device according to the acquired optical signal, wherein the optical signal is generated when the biological indicator subjected to the sterilization treatment is irradiated by the exciting light;
and monitoring the voltage value of the electric signal in real time, and judging whether the sterilization is successful according to the voltage value.
2. The method according to claim 1, wherein the step of determining whether the sterilization is successful according to the voltage value specifically comprises:
if the voltage value is larger than a set voltage threshold value and the duration time is larger than a set time threshold value within the preset time, judging that the sterilization is successful;
if not, sterilization fails.
3. The method of claim 2, further comprising:
acquiring an externally input threshold value adjusting instruction;
and adjusting the set voltage threshold or the set time threshold according to the threshold adjusting instruction.
4. A sterilization effect testing apparatus, comprising:
the instruction sending module is used for sending a control instruction to an excitation light source when detecting a detection starting signal so that the excitation light source can send out excitation light according to the control instruction;
the acquisition module is used for acquiring an electric signal output by the optical signal acquisition device according to the acquired optical signal, wherein the optical signal is generated when the biological indicator subjected to the sterilization treatment is irradiated by the exciting light;
and the monitoring processing module is used for monitoring the voltage value of the electric signal in real time and judging whether the sterilization is successful or not according to the voltage value.
5. A sterilization effectiveness verification system, comprising:
the spore culture tank is used for culturing the biological indicator after the sterilization treatment;
the optical signal acquisition device is used for acquiring optical signals generated when the biological indicator is irradiated by exciting light and converting the acquired optical signals into electric signals;
the main processor is used for sending a control instruction to the excitation light source when detecting the detection starting signal so as to enable the excitation light source to send excitation light to the biological indicator; and monitoring the voltage value of the received electric signal in real time to judge whether the sterilization is successful according to the voltage value.
6. The system of claim 5, wherein the optical signal collection device comprises: the first filter, the second filter, the silicon photodiode and the integrator;
the first filter is positioned between the excitation light source and the spore culture tank and is used for filtering the excitation light emitted by the excitation light source;
the second filter is positioned between the spore culture tank and the silicon photodiode and is used for filtering an optical signal generated by the biological indicator;
the silicon photodiode is used for converting the received optical signal into an electric signal and transmitting the electric signal to the integrator;
and the integrator is used for amplifying the electric signal.
7. The system of claim 5, further comprising: a first slave processor;
the first slave processor is used for carrying out analog-to-digital conversion processing on the received electric signals; and collecting the temperature of the spore culture tank, and controlling a heater according to the collected temperature so as to adjust the temperature of the spore culture tank.
8. The system of claim 5, further comprising: a second slave processor;
the second slave processor is respectively connected with the master singlechip, the liquid crystal display device and the button and is used for receiving monitoring data sent by the master processor through a USART (Universal asynchronous receiver transmitter) and forwarding the monitoring data to the liquid crystal display device for display; and forwarding an operation instruction input by a user through the button to the main processor through a USART transmitter so as to enable the main processor to execute corresponding operation.
9. The system of claim 5, further comprising: an EEPROM memory;
the EEPROM memory is used for storing monitoring data, and the monitoring data is transmitted to the EEPROM memory by the main processor through the SPI interface.
10. The system of any one of claims 5-9, wherein the master processor, the first slave processor, and the second slave processor are each a single-chip microcomputer.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610608239.6A CN106215209A (en) | 2016-07-28 | 2016-07-28 | A kind of the sterilization effect method of inspection, Apparatus and system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201610608239.6A CN106215209A (en) | 2016-07-28 | 2016-07-28 | A kind of the sterilization effect method of inspection, Apparatus and system |
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| CN114917371A (en) * | 2021-05-24 | 2022-08-19 | 广州市鸿利秉一光电科技有限公司 | Sterilization device and sterilization method with visible sterilization effect |
| US11565015B2 (en) | 2016-09-15 | 2023-01-31 | Asp Global Manufacturing Gmbh | Biological indicator with variable resistance |
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