CN106110367A - Based on natural polymer MULTILAYER COMPOSITE medical dressing and preparation method thereof - Google Patents
Based on natural polymer MULTILAYER COMPOSITE medical dressing and preparation method thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/22—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing macromolecular materials
- A61L15/225—Mixtures of macromolecular compounds
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
- A61L15/425—Porous materials, e.g. foams or sponges
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
- A61L15/64—Use of materials characterised by their function or physical properties specially adapted to be resorbable inside the body
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
- A61L2300/23—Carbohydrates
- A61L2300/232—Monosaccharides, disaccharides, polysaccharides, lipopolysaccharides
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/412—Tissue-regenerating or healing or proliferative agents
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Abstract
The present invention is based on natural polymer MULTILAYER COMPOSITE medical dressing and preparation method thereof.It is to be made up of MULTILAYER COMPOSITE natural polymer, by differently configured natural polymer solution, lyophilization is carried out after prefreezing, then by carrying out lyophilization after coating another one or more natural polymer solution, circulation repeatedly, obtains the MULTILAYER COMPOSITE sponge medical dressing of inierpeneirating network structure.This dressing have flexible, biocompatibility is excellent, preferable water absorption, breathability, adhesion and antibacterial action.It can absorb wound exudate, it is ensured that fully drain, can be retained in dressing by liquid permeable portion again, maintains a local moist environment imitating the healing of wound surface physiological, beneficially wound granulation tissue and re-epithelilization, accelerates wound healing.
Description
Technical field
The invention belongs to medical technical field, be specifically related to based on natural polymer MULTILAYER COMPOSITE medical dressing and preparation thereof
Methods and applications.
Technical background
Medical dressing has many inconvenience on using at present, and some dressing uses nondegradable polyacrylonitrile material to make
For support, causing iatrogenic pollution, meanwhile, dressing absorbs transudate and sticks together with wound surface simultaneously, causes damage when removing new
Raw tissue.The most gauzes made with cotton fiber, cotton balls etc., although have certain moisture absorption and protective effect, but more
Easy adhesion wound during conjunction, thus cause secondary damage, cause great misery to patient.Along with various countries' medical circle is to hemostasis
The raising that material anthemorrhagic performance requires, the bleeding-stopping dressing of exploitation excellent performance becomes a kind of trend.Owing to natural polymer has
The characteristic such as nontoxic, good biocompatibility and degradability, thus paid much attention in the research of bleeding-stopping dressing.According to document report
Road, Tang N et al. (Tang N, Cai WZ, Deng L, Li SJ, Wang XJ, Li C.Preparation and
property of closed dressing intelligent scaffolds.Zhongguo Zuzhi Gongcheng
Yanjiu yu Linchuang Kangfu.2010；14 (29): 5325-5328.) pass through with chitosan, gelatin, polyvinyl alcohol
After Gong Huning, lyophilizing is prepared for seal wound dressing for wound healing, and result shows significantly to strengthen the water suction of dressing
Property, water content and breathability, but for the biological poison of the selection of macromolecular material, the complex method of macromolecular material and material
Property be all worth further research and inquire into.
Common natural polymer mainly has: sodium alginate, chitosan, hyaluronic acid, gelatin and sodium carboxymethyl cellulose
Deng.The natural polymer that sodium alginate (Sodium Algenate, SA) is present in Brown algae, is from Brown algae or antibacterial
The natural polysaccharide extracted, its good biocompatibility, toxicity are low, have hemostasis, antibacterial, the effect of wound healing.Shell
Polysaccharide (Chitosan, CS) is widely present in the Nature, is the deacetylated derivant of natural polysaccharide, glucamine (sloughs
Aminoethyl moiety) and ethyl glucuronide amine (part of second) composition, it is a kind of environmentally friendly natural polymer.
The advantages such as chitosan not only has nontoxic, good biocompatibility, biodegradability, also have cancer resistance, antibiotic property,
Hemostatic and strengthen the plurality of advantages such as human body immunity, it is widely used in organizational project, drug carrier material and wound
The aspects such as dressing.Hyaluronic acid (Hyaluronic acid, HA) has another name called hyaluronic acid, is present in extracellular base in biological tissue
A kind of acid mucopolysaccharide in matter.By β-D-N-acetylglucosamine and β-D-Glucose sulfonic acid be construction unit with β-1,
A kind of chain macromolecule that 4-glycosidic linkage is linked to be.The biocompatibility good due to it and biological degradability and be widely used
In fields such as organizational projects.Gelatin (Gelatin, GE) is the mixtures of polypeptides of a kind of solubility, by acid or alkaline extraction, by cattle
Or collagenous portion hydrolysis system contained by porcine skin.Different with the triple-helix structure of collagen, gelatin is made up of single chain molecule.Gelatin,
Due to its biocompatibility with change with temperature and take the characteristic of gelation, it is widely used in biomedical aspect.Carboxymethyl cellulose
Element sodium (CMC) is that cellulose ether apoplexy due to endogenous wind yield is maximum, purposes is the widest, uses product the most easily.Its odorless, tasteless,
Nontoxic and hygroscopicity, has excellent water solublity, conjunction shoes, newborn, diffusibility, antienzyme and stability, extensively should
For food pharmaceuticals industry.
The present invention, by differently configured natural polymer solution, prepares MULTILAYER COMPOSITE by multistep freeze seasoning natural
Macromolecule medical dressing, its have flexible, biocompatibility is excellent, preferable water absorption, breathability, adhesion and antibacterial work
With.It can absorb wound exudate, it is ensured that fully drain, can be retained in dressing by liquid permeable portion again, maintains one to imitate wound
The local moist environment of rationality of looking unfamiliar healing, beneficially wound granulation tissue and re-epithelilization, accelerates wound healing.
Summary of the invention
It is an object of the invention to, based on natural polymer MULTILAYER COMPOSITE medical dressing and preparation method thereof, use above-mentioned system
The dressing that Preparation Method obtains has natural polymer base, compound layer by layer and inierpeneirating network structure and has good biofacies
Capacitive, breathability, poisture-penetrability, absorbency and water retainability.
Realize above-mentioned purpose, the following technical scheme that the present invention uses:
(1) preparation of sodium alginate soln: be dissolved in distilled water by sodium alginate, being configured to mass fraction is 1～4%
Sodium alginate soln, with motor stirrer stir to being completely dissolved, vacuum defoamation 24h, place stand-by.
(2) preparation of natural polymer solution: natural polymer is dissolved respectively, be configured to mass fraction be 1～
The natural polymer solution of 4%, stirs to being completely dissolved with motor stirrer, vacuum defoamation 24h, places stand-by.
(3) preparation of calcium alginate lyophilizing sponge: the sodium alginate soln configured is injected in mould ,-20～-70 DEG C
Low temperature precooling 1～5h, then in-80～-10 DEG C of temperature, in 1～600Pa vacuum, the sodium alginate soln that will freeze
Lyophilization 24～48h in freezer dryer, obtain sodium alginate lyophilizing sponge, then soak in cross-linking agent solution 1～5%
Bubble 1-5h cross-links, and distilled water cleans, and is then dried to horizontal heavy in 50～80 DEG C of baking ovens, obtains calcium alginate sponge.
(4) preparation based on natural polymer MULTILAYER COMPOSITE medical dressing: by preprepared natural polymer solution
It is coated on calcium alginate lyophilizing sponge side ,-20～-70 DEG C of low temperature precoolings 1～5h, then in-80～-10 DEG C of temperature, 1
～in 600Pa vacuum, it is coated with calcium alginate sponge lyophilization 24 in freezer dryer of natural polymer solution
～48h, obtaining its ground floor of lyophilizing composite sponge is alginic acid calcium layer, and the second layer is single or multiple lift natural polymer, then
Sodium alginate soln is coated on natural polymer sublayer side again, and-20～-70 DEG C of low temperature precoolings 1～5h, then-80
～in-10 DEG C of temperature, in 1～600Pa vacuum, by MULTILAYER COMPOSITE sponge in freezer dryer after lyophilization 24～48h
Soaking 1-5h in cross-linking agent solution 1～5% to cross-link, distilled water cleans, and is then dried in 50～80 DEG C of baking ovens to horizontal heavy,
Obtaining ground floor is calcium alginate, and the second layer is single or multiple lift natural polymer, and third layer is the MULTILAYER COMPOSITE of calcium alginate
Medical dressing.
Specifically, one during described natural polymer is chitosan, hyaluronic acid, gelatin and sodium carboxymethyl cellulose or
Several.
Specifically, described second layer natural polymer sublayer is in chitosan, hyaluronic acid, gelatin and sodium carboxymethyl cellulose
Single one or more be combined layer by layer.
Specifically, described cryogenic temperature-80～-10 DEG C, vacuum 1～600Pa, cooling time 12～48h.
Specifically, described sodium alginate molecular weight Mw=2 × 103-40×103g·mol-1, chitosan molecule amount Mw=1
×104-3×104g·mol-1, hyaluronan molecule amount Mw=8 × 105-12×105g·mol-1, gelatine molecular weight Mw=6 ×
104-30×104g·mol-1, sodium carboxymethyl cellulose molecular weight Mw=1.8 × 104-2.8×104g·mol-1。
Specifically, described cryogenic temperature-80～-10 DEG C, vacuum 1～600Pa, cooling time 12～48h.
Specifically, one or more during described cross-linking agent solution is the ethanol solution of the aqueous solution of calcium chloride, calcium chloride.
Specifically, the medical dressing of described preparation is multilamellar natural polymer composite sponge, and this dressing can be used for biological doctor
Medicine technical field.
The invention have the advantages that
1. the preparation method of the MULTILAYER COMPOSITE sponge medical dressing of the present invention uses natural polymer to be raw material, by freezing
The technology being dried, with calcium chloride as cross-linking agent, preparing ground floor is calcium alginate, and the second layer is single or multiple lift natural polymer
Son, third layer is the medical dressing sponge of calcium alginate.This dressing all uses natural polymer to be base material, has nontoxic, raw
Thing degraded, biocompatibility and being readily available property and the method using MULTILAYER COMPOSITE, preparation technology is novel, easy, produce into
This is low, it is simple to promoting the use of of medical dressing prepared by natural polymer.
2. the MULTILAYER COMPOSITE dressing sponge that prepared by the method is better than simple natural polymer dressing sponge and the most common
Mixed dressing sponge, effectively can substitute traditional wound dressing, is used for treating surgical wound, empyrosis wound surface etc. each
The skin trauma of type.
Accompanying drawing explanation
Fig. 1 is the structural representation of MULTILAYER COMPOSITE medical dressing；
In figure: alginic acid calcium layer 1, single or multiple lift natural polymer sublayer 2, alginic acid calcium layer 3.
Fig. 2 is the top view digital photograph of MULTILAYER COMPOSITE medical dressing；
Fig. 3 is the configuration of surface scanning electron microscope (SEM) photograph of MULTILAYER COMPOSITE medical dressing；
Fig. 4 is the front view digital photograph of MULTILAYER COMPOSITE medical dressing；
Fig. 5 is the configuration of surface scanning electron microscope (SEM) photograph of MULTILAYER COMPOSITE medical dressing；
Fig. 6 is the cross-sectional scans Electronic Speculum figure of MULTILAYER COMPOSITE medical dressing.
Specific embodiment
Embodiment one
1g sodium alginate is dissolved in 100g distilled water, is configured to the sodium alginate soln that mass fraction is 1%, electricity consumption
Dynamic agitator stirs to being completely dissolved, and vacuum defoamation 24h is standby.1g chitosan is dissolved in 100g 1% (v/v) formic acid solution
In, it is configured to the chitosan solution of 1%, is sufficiently stirred for making it uniform, standby.Sodium alginate soln is dispensed in mould, will
Mould is placed in pre-freeze in cryogenic refrigerator ,-20 DEG C of pre-freeze 1h, is then placed in freezer dryer by mould, controls in dry run
Vacuum is less than below 15Pa, processes 24h in-50 DEG C, and the dried demoulding obtains the sodium alginate sponge being dried, then 1%
Crosslinking curing 2h in calcium chloride ethanol solution, is placed on sponge after having solidified in 50 DEG C of vacuum drying ovens and is dried to constant weight, distillation
Water is dried to constant weight after cleaning repeatedly in 50 DEG C of vacuum drying ovens, obtains calcium alginate lyophilizing sponge, standby.Chitosan is molten
Liquid is coated on this lyophilizing sponge side of alginic acid ,-20 DEG C of low temperature precooling 1h, it is placed in freezer dryer, controls dry run
Middle vacuum is less than below 15Pa, processes 24h in-50 DEG C, and obtaining its first top layer of lyophilizing composite sponge is alginic acid calcium layer, the
Two layers is chitosan layer.Then sodium alginate soln is coated on chitosan side ,-20 DEG C of low temperature precooling 1h, is placed in freezing dry
In dry machine, control vacuum in dry run and, less than below 15Pa, process 24h in-50 DEG C, in 1% calcium chloride ethanol solution
Crosslinking curing 2h, is placed on sponge after having solidified in 50 DEG C of vacuum drying ovens and is dried to constant weight, in 50 after distilled water cleaning repeatedly
DEG C vacuum drying oven is dried to constant weight, obtain ground floor be calcium alginate, the second layer be chitosan, third layer be calcium alginate
MULTILAYER COMPOSITE medical dressing sponge (Fig. 1).Digital camera carries out pictorial diagram shooting top view (Fig. 2).Seen by scanning electron microscope
Examine and utilize the medical dressing prepared by case study on implementation 1 to show microscopic appearance (Fig. 3).
Embodiment two
2g sodium alginate is dissolved in 100g distilled water, is configured to the sodium alginate soln that mass fraction is 2%, electricity consumption
Dynamic agitator stirs to being completely dissolved, and vacuum defoamation 24h is standby.2g gelatin is dissolved in 100g distilled water, is configured to 2%
Gelatin solution, is sufficiently stirred for making it uniform, standby.Sodium alginate soln is dispensed in mould, mould is placed in cryogenic refrigerator
Middle pre-freeze ,-30 DEG C of pre-freeze 2h, then mould is placed in freezer dryer, control in dry run vacuum less than 20Pa with
Under, processing 28h in-40 DEG C, the dried demoulding obtains the sodium alginate sponge being dried, and then hands in 2% calcium chloride water
Connection solidification 1h, is placed on sponge after having solidified in 50 DEG C of vacuum drying ovens and is dried to constant weight, in 50 DEG C after distilled water cleaning repeatedly
Vacuum drying oven is dried to constant weight and obtains calcium alginate sponge.Gelatin solution is coated on calcium alginate lyophilizing sponge side ,-
30 DEG C of low temperature precooling 2h, are placed in freezer dryer, control vacuum in dry run and are less than below 20Pa, at-40 DEG C
Reason 28h, obtaining its first top layer of lyophilizing composite sponge is alginic acid calcium layer, and the second layer is gelatin layer.Finally that sodium alginate is molten
Liquid is coated on gelatin side ,-30 DEG C of low temperature precooling 2h, is placed in freezer dryer, controls vacuum in dry run and is less than
Below 20Pa, processes 28h in-40 DEG C, and in 2% calcium chloride water, crosslinking curing 1h, is placed on 50 by sponge after having solidified
Being dried in DEG C vacuum drying oven to constant weight, distilled water is dried to constant weight in 50 DEG C of vacuum drying ovens after cleaning repeatedly, obtains first
The MULTILAYER COMPOSITE medical dressing sponge that layer is calcium alginate, the second layer is gelatin, third layer is calcium alginate.Digital camera is carried out
Pictorial diagram shooting front view (Fig. 4).The medical dressing prepared by case study on implementation 2 is utilized to show microcosmic shape by scanning electron microscopic observation
Looks (Fig. 5).The cross section microscopic appearance (Fig. 6) of medical dressing prepared by case study on implementation is utilized by scanning electron microscopic observation.
Embodiment three
3g sodium alginate is dissolved in 100g distilled water, is configured to the sodium alginate soln that mass fraction is 3%, electricity consumption
Dynamic agitator stirs to being completely dissolved, and vacuum defoamation 24h is standby.3g chitosan is dissolved in 100g 1% (v/v) formic acid solution
In, it is configured to the chitosan solution of 3%, is sufficiently stirred for making it the most standby.3g sodium carboxymethyl cellulose is dissolved in 100g distillation
In water, it is configured to the carboxymethylcellulose sodium solution of 3%, is sufficiently stirred for making it uniform, standby.Sodium alginate soln is dispensed into
In mould, mould is placed in pre-freeze in cryogenic refrigerator ,-40 DEG C of pre-freeze 3h, then mould is placed in freezer dryer, control dry
During dry, vacuum is less than below 30Pa, processes 30h in-60 DEG C, and the dried demoulding obtains the sodium alginate sponge being dried, so
After in 3% calcium chloride water crosslinking curing 3h, sponge is placed in 50 DEG C of vacuum drying ovens after having solidified and is dried to constant weight,
Distilled water is dried to constant weight after cleaning repeatedly in 50 DEG C of vacuum drying ovens, obtains calcium alginate lyophilizing sponge.Chitosan is molten
Liquid is coated on calcium alginate lyophilizing sponge side ,-40 DEG C of low temperature precooling 3h, is placed in freezer dryer, controls dry run
Middle vacuum is less than below 30Pa, processes 30h in-60 DEG C, and obtaining its first top layer of lyophilizing composite sponge is sodium alginate layer, the
Two layers is chitosan layer.Then carboxymethylcellulose sodium solution is coated on chitosan side ,-40 DEG C of low temperature precooling 3h, puts
In freezer dryer, control vacuum in dry run and, less than below 30Pa, process 30h in-60 DEG C, obtain ground floor for sea
Sodium alginate, the second layer are the composite sponge of chitosan-sodium carboxymethyl cellulose.Finally sodium alginate soln is coated on carboxymethyl
Sodium cellulosate side ,-40 DEG C of low temperature precooling 3h, it is placed in freezer dryer, controls vacuum in dry run and be less than 30Pa
Hereinafter, process 30h, crosslinking curing 3h in 3% calcium chloride water in-60 DEG C, after having solidified, sponge is placed on 50 DEG C very
Being dried in empty baking oven to constant weight, distilled water is dried to constant weight after cleaning repeatedly in 50 DEG C of vacuum drying ovens, and obtaining ground floor is
Calcium alginate, the second layer be chitosan-carboxymethyl cellulose sodium, third layer be the MULTILAYER COMPOSITE medical dressing sponge of calcium alginate.
Embodiment four
4g sodium alginate is dissolved in 100g distilled water, is configured to the sodium alginate soln that mass fraction is 4%, electricity consumption
Dynamic agitator stirs to being completely dissolved, and vacuum defoamation 24h is standby.4g hyaluronic acid is dissolved in 100g distilled water, is configured to
The hyaluronic acid solution of 4%, is sufficiently stirred for making it uniform, standby.4g gelatin is dissolved in 100g distilled water, is configured to 4%
Gelatin solution, is sufficiently stirred for making it uniform, standby.Sodium alginate soln is dispensed in mould, mould is placed in cryogenic refrigerator
Middle pre-freeze ,-50 DEG C of low temperature precooling 4h, then mould is placed in freezer dryer, controls vacuum in dry run and be less than
Below 15Pa, processes 24h in-50 DEG C, and the dried demoulding obtains the sodium alginate sponge being dried, then at 1% calcium chloride ethanol
Crosslinking curing 2h in solution, is placed on sponge in 50 DEG C of vacuum drying ovens after having solidified and is dried to constant weight, obtain calcium alginate and freeze
Dry sponge.To hyaluronic acid solution being coated on calcium alginate lyophilizing sponge side ,-50 DEG C of low temperature precooling 4h, it is placed in freezing
In drying machine, control vacuum in dry run and, less than below 15Pa, process 24h in-50 DEG C, obtain lyophilizing composite sponge its
One top layer is sodium alginate layer, and the second layer is hyaluronic acid.Then gelatin solution is coated on hyaluronic acid side ,-50 DEG C
Low temperature precooling 4h, is placed in freezer dryer, controls vacuum in dry run and is less than below 15Pa, in-50 DEG C of process
24h, obtain ground floor be sodium alginate, the second layer be the composite sponge of hyaluronic acid-gelatin.Finally sodium alginate soln is coated with
It is overlying on gelatin side ,-50 DEG C of low temperature precooling 4h, is placed in freezer dryer, control vacuum in dry run and be less than 15Pa
Hereinafter, process 24h, crosslinking curing 2h in 1% calcium chloride ethanol solution in-50 DEG C, after having solidified, sponge is placed on 50 DEG C
Vacuum drying oven is dried to constant weight, obtain ground floor be calcium alginate, the second layer be hyaluronic acid-gelatin, third layer be Sargassum
The MULTILAYER COMPOSITE medical dressing sponge of acid calcium.
Embodiment five
1g sodium alginate is dissolved in 100g distilled water, is configured to the sodium alginate soln that mass fraction is 1%, electricity consumption
Dynamic agitator stirs to being completely dissolved, and vacuum defoamation 24h is standby.1g sodium carboxymethyl cellulose is dissolved in 100g distilled water, joins
It is set to the carboxymethylcellulose sodium solution of 1%, is sufficiently stirred for making it uniform, standby.1g gelatin is dissolved in 100g distilled water, joins
It is set to the gelatin solution of 1%, is sufficiently stirred for making it uniform, standby.Sodium alginate soln is dispensed in mould, mould is placed in
Pre-freeze in cryogenic refrigerator ,-60 DEG C of low temperature precooling 5h, then mould is placed in freezer dryer, controls in dry run true
Reciprocal of duty cycle is less than below 15Pa, processes 24h in-50 DEG C, and the dried demoulding obtains the sodium alginate sponge being dried, then at 1% chlorine
Change crosslinking curing 2h in calcium ethanol solution, sponge is placed in 50 DEG C of vacuum drying ovens after having solidified and is dried to constant weight, distilled water
It is dried to constant weight in 50 DEG C of vacuum drying ovens after cleaning repeatedly, obtains calcium alginate lyophilizing sponge.By sodium carboxymethyl cellulose
Solution is coated on this lyophilizing sponge side of alginic acid ,-60 DEG C of low temperature precooling 5h, is placed in freezer dryer, controls dried
In journey, vacuum is less than below 15Pa, processes 24h in-50 DEG C, and obtaining its first top layer of lyophilizing composite sponge is sodium alginate layer,
The second layer is sodium carboxymethyl cellulose layer.Then gelatin solution is coated on sodium carboxymethyl cellulose side ,-60 DEG C of low temperature pre-coolings
Freeze 5h, be placed in freezer dryer, control vacuum in dry run and, less than below 15Pa, process 24h in-50 DEG C, obtain the
One layer is composite sponge that sodium alginate, the second layer are sodium carboxymethyl cellulose-gelatin.Finally sodium alginate soln is coated on
Gelatin side ,-60 DEG C of low temperature precooling 5h, it is placed in freezer dryer, controls vacuum in dry run and be less than below 15Pa,
Process 24h, crosslinking curing 2h in 1% calcium chloride ethanol solution in-50 DEG C, after having solidified, sponge is placed on 50 DEG C of vacuum and dries
Being dried in case to constant weight, distilled water is dried to constant weight after cleaning repeatedly in 50 DEG C of vacuum drying ovens, and obtaining ground floor is Sargassum
Acid calcium, the second layer be sodium carboxymethyl cellulose-gelatin, third layer be the MULTILAYER COMPOSITE medical dressing sponge of calcium alginate.
Embodiment six
1g sodium alginate is dissolved in 100g distilled water, is configured to the sodium alginate soln that mass fraction is 1%, electricity consumption
Dynamic agitator stirs to being completely dissolved, and vacuum defoamation 24h is standby.1g chitosan is dissolved in 100g 1% (v/v) formic acid solution
In, it is configured to the chitosan solution of 1%, is sufficiently stirred for making it uniform, standby.1g sodium carboxymethyl cellulose is dissolved in 100g distillation
In water, it is configured to the carboxymethylcellulose sodium solution of 1%, is sufficiently stirred for making it uniform, standby.1g gelatin is dissolved in 100g distillation
In water, it is configured to the gelatin solution of 1%, is sufficiently stirred for making it uniform, standby.Sodium alginate soln is dispensed in mould, will
Mould is placed in pre-freeze in cryogenic refrigerator ,-70 DEG C of pre-freeze 1h, is then placed in freezer dryer by mould, controls in dry run
Vacuum is less than below 15Pa, processes 24h in-50 DEG C, and the dried demoulding obtains the sodium alginate sponge being dried, then 1%
Crosslinking curing 2h in calcium chloride ethanol solution, is placed on sponge after having solidified in 50 DEG C of vacuum drying ovens and is dried to constant weight, distillation
Water is dried to constant weight after cleaning repeatedly in 50 DEG C of vacuum drying ovens, obtains calcium alginate lyophilizing sponge, standby.Chitosan is molten
Liquid is coated on this lyophilizing sponge side of alginic acid ,-70 DEG C of low temperature precooling 1h, is placed in freezer dryer, controls dry run
Middle vacuum is less than below 15Pa, processes 24h in-50 DEG C, and obtaining its first top layer of lyophilizing composite sponge is alginic acid calcium layer, the
Two layers is chitosan layer.Then carboxymethylcellulose sodium solution is coated on chitosan side ,-70 DEG C of low temperature precooling 1h, is placed in
In freezer dryer, controlling vacuum in dry run and, less than below 15Pa, process 24h in-50 DEG C, obtaining ground floor is Sargassum
Acid calcium, the MULTILAYER COMPOSITE sponge of the second layer-sodium carboxymethyl cellulose.Gelatin solution is coated on sodium carboxymethyl cellulose side ,-
70 DEG C of low temperature precooling 1h, are placed in freezer dryer, control vacuum in dry run and are less than below 15Pa, at-50 DEG C
Reason 24h, obtaining ground floor is calcium alginate, the MULTILAYER COMPOSITE sponge of the second layer-sodium carboxymethyl cellulose-gelatin.Finally by sea
Solution of sodium alginate is coated on gelatin side ,-70 DEG C of low temperature precooling 1h, is placed in freezer dryer, controls in dry run true
Reciprocal of duty cycle is less than below 15Pa, processes 24h, crosslinking curing 2h in 1% calcium chloride ethanol solution in-50 DEG C, will after having solidified
Sponge is placed in 50 DEG C of vacuum drying ovens and is dried to constant weight, and distilled water is dried to perseverance after cleaning repeatedly in 50 DEG C of vacuum drying ovens
Weight, obtain ground floor be calcium alginate, the second layer be chitosan-sodium carboxymethyl cellulose-gelatin, third layer be calcium alginate
MULTILAYER COMPOSITE medical dressing sponge.
Embodiment seven
2g sodium alginate is dissolved in 100g distilled water, is configured to the sodium alginate soln that mass fraction is 2%, electricity consumption
Dynamic agitator stirs to being completely dissolved, and vacuum defoamation 24h is standby.2g gelatin is dissolved in 100g distilled water, is configured to 2%
Gelatin solution, is sufficiently stirred for making it uniform, standby.2g hyaluronic acid is dissolved in 100g distilled water, is configured to the hyalomitome of 2%
Acid solution, is sufficiently stirred for making it uniform, standby.2g sodium carboxymethyl cellulose is dissolved in 100g distilled water, is configured to the carboxylic of 2%
Methylcellulose sodium solution, is sufficiently stirred for making it uniform, standby.Sodium alginate soln is dispensed in mould, mould is placed in
Pre-freeze in cryogenic refrigerator ,-30 DEG C of pre-freeze 2h, then mould is placed in freezer dryer, controls vacuum in dry run low
In below 20Pa, processing 28h in-40 DEG C, the dried demoulding obtains the sodium alginate sponge being dried, then at 2% calcium chloride water
Crosslinking curing 1h in solution, is placed on sponge after having solidified in 50 DEG C of vacuum drying ovens and is dried to constant weight, and distilled water cleans repeatedly
After be dried in 50 DEG C of vacuum drying ovens to constant weight and obtain calcium alginate sponge.Gelatin solution is coated on calcium alginate lyophilizing sea
Continuous side ,-30 DEG C of pre-freeze 2h, it is placed in freezer dryer, controls vacuum in dry run and be less than below 20Pa, in-40 DEG C
Processing 28h, obtaining its first top layer of lyophilizing composite sponge is alginic acid calcium layer, and the second layer is gelatin layer.Then hyaluronic acid is molten
Liquid is coated on gelatin side, is placed in freezer dryer, controls vacuum in dry run and is less than below 15Pa, at-50 DEG C
Reason 24h, obtain ground floor be calcium alginate, the second layer be the MULTILAYER COMPOSITE sponge of gelatin-hyaluronic acid.By carboxymethyl cellulose
Sodium solution is coated on sodium carboxymethyl cellulose side ,-30 DEG C of pre-freeze 2h, is placed in freezer dryer, controls in dry run true
Reciprocal of duty cycle is less than below 15Pa, processes 24h in-50 DEG C, obtain ground floor be calcium alginate, the second layer be gelatin-hyaluronic acid-carboxylic
The MULTILAYER COMPOSITE sponge of sodium carboxymethylcellulose pyce.Finally sodium alginate soln is coated on gelatin side ,-30 DEG C of pre-freeze 2h, is placed in
In freezer dryer, control vacuum in dry run and, less than below 20Pa, process 28h in-40 DEG C, water-soluble at 2% calcium chloride
Crosslinking curing 1h in liquid, is placed on sponge after having solidified in 50 DEG C of vacuum drying ovens and is dried to constant weight, after distilled water cleans repeatedly
Be dried to constant weight in 50 DEG C of vacuum drying ovens, obtain ground floor be calcium alginate, the second layer be gelatin-hyaluronic acid-carboxylic first
Base sodium cellulosate, third layer are the MULTILAYER COMPOSITE medical dressing sponge of calcium alginate.
Embodiment eight
4g sodium alginate is dissolved in 100g distilled water, is configured to the sodium alginate soln that mass fraction is 4%, electricity consumption
Dynamic agitator stirs to being completely dissolved, and vacuum defoamation 24h is standby.4g chitosan is dissolved in 100g 1% (v/v) formic acid solution
In, it is configured to the chitosan solution of 4%, is sufficiently stirred for making it uniform, standby.4g hyaluronic acid is dissolved in 100g distilled water,
It is configured to the hyaluronic acid solution of 4%, is sufficiently stirred for making it uniform, standby.4g gelatin is dissolved in 100g distilled water, is configured to
The gelatin solution of 4%, is sufficiently stirred for making it uniform, standby.4g sodium carboxymethyl cellulose is dissolved in 100g distilled water, is configured to
The carboxymethylcellulose sodium solution of 4%, is sufficiently stirred for making it uniform, standby.Sodium alginate soln is dispensed in mould, by mould
Have and be placed in pre-freeze in cryogenic refrigerator ,-50 DEG C of low temperature precooling 4h, then mould is placed in freezer dryer, controls dried
In journey, vacuum is less than below 15Pa, processes 24h in-50 DEG C, and the dried demoulding obtains the sodium alginate sponge being dried, then exists
Crosslinking curing 2h in 1% calcium chloride ethanol solution, is placed on sponge in 50 DEG C of vacuum drying ovens after having solidified and is dried to constant weight,
Calcium alginate lyophilizing sponge.Chitosan solution is coated on calcium alginate lyophilizing sponge side ,-50 DEG C of low temperature precooling 4h, puts
In freezer dryer, control vacuum in dry run and, less than below 15Pa, process 24h in-50 DEG C, obtain lyophilizing compounding sea
Its first top layer continuous is sodium alginate layer, and the second layer is chitosan layer.Then hyaluronic acid solution is coated on chitosan one
Side ,-50 DEG C of low temperature precooling 4h, it is placed in freezer dryer, controls vacuum in dry run and be less than below 15Pa, in-50
DEG C process 24h, obtain ground floor be sodium alginate, the second layer be the composite sponge of Chitosan-Hyaluronic Acid.Then gelatin is molten
Liquid is coated on hyaluronic acid side ,-50 DEG C of low temperature precooling 4h, is placed in freezer dryer, controls vacuum in dry run
Less than below 15Pa, process 24h in-50 DEG C, obtain ground floor be sodium alginate, the second layer be Chitosan-Hyaluronic Acid-gelatin
Composite sponge.Then carboxymethylcellulose sodium solution is coated on gelatin side ,-50 DEG C of low temperature precooling 4h, is placed in freezing
In drying machine, controlling vacuum in dry run and, less than below 15Pa, process 24h in-50 DEG C, obtaining ground floor is alginic acid
Sodium, the second layer are the composite sponge of Chitosan-Hyaluronic Acid-gelatin-sodium carboxymethyl cellulose.Finally sodium alginate soln is coated with
It is overlying on sodium carboxymethyl cellulose side ,-50 DEG C of low temperature precooling 4h, is placed in freezer dryer, control vacuum in dry run
Degree, less than below 15Pa, processes 24h in-50 DEG C, and crosslinking curing 2h in 1% calcium chloride ethanol solution, by sea after having solidified
Silk floss is placed in 50 DEG C of vacuum drying ovens and is dried to constant weight, obtain ground floor be calcium alginate, the second layer be Chitosan-Hyaluronic Acid-
Gelatin-sodium carboxymethyl cellulose, third layer are the MULTILAYER COMPOSITE medical dressing sponge of calcium alginate.
Claims (7)
1. based on natural polymer MULTILAYER COMPOSITE medical dressing and preparation method thereof, it is characterized in that: this dressing is by the natural height of multilamellar
Molecule is compound to be constituted, wherein ground floor be calcium alginate, the second layer be single or multiple lift natural polymer, third layer be alginic acid
Calcium, this preparation method comprises the following steps:
(1) preparation of sodium alginate soln: be dissolved in distilled water by sodium alginate, is configured to the sea that mass fraction is 1～4%
Solution of sodium alginate, stirs to being completely dissolved with motor stirrer, vacuum defoamation 24h, places stand-by；
(2) preparation of natural polymer solution: dissolved respectively by natural polymer, being configured to mass fraction is 1～4%
Natural polymer solution, stirs to being completely dissolved with motor stirrer, vacuum defoamation 24h, places stand-by；
(3) preparation of calcium alginate lyophilizing sponge: the sodium alginate soln configured is injected in mould ,-20～-70 DEG C of low temperature
Precooling 1～5h, then in-80～-10 DEG C of temperature, in 1～600Pa vacuum, by the sodium alginate soln that freezes cold
Lyophilization 24～48h in lyophilizer, obtain sodium alginate lyophilizing sponge, then soak 1-1～5% in cross-linking agent solution
5h cross-links, and distilled water cleans, and is then dried to horizontal heavy in 50～80 DEG C of baking ovens, obtains calcium alginate sponge；
(4) preparation based on natural polymer MULTILAYER COMPOSITE medical dressing: preprepared natural polymer solution is coated
In calcium alginate lyophilizing sponge side ,-20～-70 DEG C of low temperature precoolings 1～5h, then in-80～-10 DEG C of temperature, 1～
In 600Pa vacuum, be coated with the calcium alginate sponge of natural polymer solution in freezer dryer lyophilization 24～
48h, obtaining its ground floor of lyophilizing composite sponge is alginic acid calcium layer, and the second layer is single or multiple lift natural polymer, then will
Sodium alginate soln is coated on single or multiple lift natural polymer sublayer side again ,-20～-70 DEG C of low temperature precoolings 1～5h, so
After in-80～-10 DEG C of temperature, in 1～600Pa vacuum, by MULTILAYER COMPOSITE sponge lyophilization 24 in freezer dryer
～1～5%, cross-linking agent solution soaks 1-5h after 48h and cross-link, distilled water cleans, and is then dried in 50～80 DEG C of baking ovens
To horizontal heavy, obtaining ground floor is calcium alginate, and the second layer is single or multiple lift natural polymer, and third layer is that calcium alginate is many
Layer based composite dressing for medical use.
The most according to claim 1 based on natural polymer MULTILAYER COMPOSITE medical dressing and preparation method thereof, its feature exists
One or more in described natural polymer is chitosan, hyaluronic acid, gelatin and sodium carboxymethyl cellulose.
The most according to claim 1 based on natural polymer MULTILAYER COMPOSITE medical dressing and preparation method thereof, its feature exists
It is single one or several in chitosan, hyaluronic acid, gelatin and sodium carboxymethyl cellulose in described second layer natural polymer sublayer
Plant and be combined layer by layer.
4. according to described in claim 1 and 2 based on natural polymer MULTILAYER COMPOSITE medical dressing and preparation method thereof, its feature
It is described sodium alginate molecular weight Mw=2 × 103-40×103g·mol-1, chitosan molecule amount Mw=1 × 104-3×
104g·mol-1, hyaluronan molecule amount Mw=8 × 105-12×105g·mol-1, gelatine molecular weight Mw=6 × 104-30×
104g·mol-1, sodium carboxymethyl cellulose molecular weight Mw=1.8 × 104—2.8×104g·mol-1。
The most according to claim 1 based on natural polymer MULTILAYER COMPOSITE medical dressing and preparation method thereof, its feature exists
In the cryogenic temperature-80 described in above-mentioned steps (3)～-10 DEG C, vacuum 1～600Pa, cooling time 12～48h.
The most according to claim 1 based on natural polymer MULTILAYER COMPOSITE medical dressing and preparation method thereof, its feature exists
One or more in cross-linking agent solution is the ethanol solution of the aqueous solution of calcium chloride, calcium chloride described in above-mentioned steps (3).
The most according to claim 1 based on natural polymer MULTILAYER COMPOSITE medical dressing and preparation method thereof, its feature exists
Medical dressing in described preparation is multilamellar natural polymer composite sponge, and this dressing can be used for biomedicine technical field.
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Cited By (5)
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| CN108066807A (en) * | 2018-02-07 | 2018-05-25 | 苏州元禾医疗器械有限公司 | The preparation method of the absorption styptic dressing of high imbibition ability |
| CN110448714A (en) * | 2019-09-23 | 2019-11-15 | 南通大学 | A kind of antibacterial chitosan of moisture absorption/sodium alginate intertexture type dressing and preparation method |
| WO2020062282A1 (en) * | 2018-09-30 | 2020-04-02 | 成都远山原力生物科技有限公司 | Low-temperature preservation solution for cells, sodium alginate gel, cell gel, preparation method, use method and application thereof |
| CN114177346A (en) * | 2021-12-24 | 2022-03-15 | 中国人民解放军军事科学院军事医学研究院 | Hemostatic composition, hemostatic patch and application thereof |
| CN114681654A (en) * | 2020-12-31 | 2022-07-01 | 广州迈普再生医学科技股份有限公司 | Absorbable sponge dressing with wound repair function and preparation method thereof |
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| CN1774272A (en) * | 2000-12-07 | 2006-05-17 | 株式会社日本组织工程 | Substrate for tissue regeneration, material for transplantation, and processes for producing these |
| WO2010084481A1 (en) * | 2009-01-23 | 2010-07-29 | Royal College Of Surgeons In Ireland | Layered scaffold suitable for osteochondral repair |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108066807A (en) * | 2018-02-07 | 2018-05-25 | 苏州元禾医疗器械有限公司 | The preparation method of the absorption styptic dressing of high imbibition ability |
| WO2020062282A1 (en) * | 2018-09-30 | 2020-04-02 | 成都远山原力生物科技有限公司 | Low-temperature preservation solution for cells, sodium alginate gel, cell gel, preparation method, use method and application thereof |
| CN110448714A (en) * | 2019-09-23 | 2019-11-15 | 南通大学 | A kind of antibacterial chitosan of moisture absorption/sodium alginate intertexture type dressing and preparation method |
| CN110448714B (en) * | 2019-09-23 | 2022-02-25 | 南通大学 | Moisture-absorbing and bacteriostatic chitosan/sodium alginate interwoven dressing and preparation method thereof |
| CN114681654A (en) * | 2020-12-31 | 2022-07-01 | 广州迈普再生医学科技股份有限公司 | Absorbable sponge dressing with wound repair function and preparation method thereof |
| CN114177346A (en) * | 2021-12-24 | 2022-03-15 | 中国人民解放军军事科学院军事医学研究院 | Hemostatic composition, hemostatic patch and application thereof |
| CN114177346B (en) * | 2021-12-24 | 2023-05-26 | 中国人民解放军军事科学院军事医学研究院 | Hemostatic composition and hemostatic patch and application thereof |
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