CN106071143B - A kind of granular pattern probiotics and preparation method thereof that ruminant production performance can be improved - Google Patents
A kind of granular pattern probiotics and preparation method thereof that ruminant production performance can be improved Download PDFInfo
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Abstract
The invention discloses a kind of granular pattern probiotics that ruminant production performance can be improved, formed by the bacillus subtilis mixing granulation that 25-35 parts of saccharomyces cerevisiae solid culture that deposit number is CCTCC NO:M 2015209,15-25 parts of the aspergillus niger solid culture that deposit number is CCTCC NO:M 2015722,15-25 parts of the aspergillus oryzae solid culture that bacterium numbering is CICC 2174,25-35 parts of the lactobacillus plantarum solid culture that deposit number is CCTCC M 2010150 and deposit number are CCTCC M 2010260.Invention additionally discloses the preparation methods of the granular pattern probiotics.Granular pattern probiotics of the invention can both well solve microbial forage additive in feed granulating the problem of loss of activity and the problem of powder-type feed addictive and granular pattern feed cannot mix；Nutrition well can be provided again for animal and play the beneficial effect of probiotics, have effects that improve ruminant production performance.
Description
Technical field
The present invention relates to a kind of granular pattern probiotics and preparation method thereof that ruminant production performance can be improved, and belong to
In microbial forage additive technical field.
Background technique
Powder refers to the powder mixed after cereal milling plus chaff money, fish meal, mineral powders and various additives etc.
Shape feed.The advantages of powder be preparation method is simple, production cost is low, animal be not easy it is particular about food；But powder palatability is weaker, and
And be easy to disperse, it causes to waste.Pellet is that diameter is made with granulator is 2.5-5.0 millimeters to the powder that will have been worked good
Particle.This pellet advantage for powder is that nutrition is perfect, and feed digestion absorption rate is high, and palatability is strong,
It can avoid waste to avoid partial eclipse, be convenient for mechanization feeding, save labour, be more suitable for the requirement of modernization, mechanization.
Pellet is widely used in piglet, broiler chicken, fishes and shrimps and experimental animal production at present.With process equipment day
Benefit is improved, and the range of increasingly improving for processing technology level and further decreasing for cost, the application of this feed processing technology will
It is increasing.It is fairly common in American-European some countries with pellet breeding milk cow, beef cattle and other ruminants.Using particle
Material daily ration has adapted to current ruminant aquaculture to intensive, scale, the needs of high-quality and efficient operation and development, is conducive to raise
The development and utilization for expecting resource is conducive to have using modern ruminant animal nutrition regulation theory and technology production commercialization daily ration
The situation to relatively lag behind is produced conducive to ruminant feed is fundamentally changed, reduces the gap with herding developed country.Therefore,
Particulate material breeding technology is undoubtedly current China ruminant breeding technology and the improved direction of system.
In China, due to technology, cost etc., ruminant is mainly using the yard feeding pattern of traditional smart rough segmentation feeding.
Currently, corpuscular feed has been widely used in the ruminant breeding process in some areas, and due to current Tiny ecosystem
Probiotics in feed addictive is in addition to bacillus due to the high-temperature maturing not being resistant in granular pattern feed preparation
Death, so current microbial forage additive is pulvis, and the feed addictive of powder-type is mixed with granular pattern feed
Lower layer can be fallen on when even use by particulate material gap, thus can not mix and use with granular pattern feed.Therefore, it develops a kind of suitable
Granular pattern probiotics for ruminant are a technical problem to be solved urgently.
Summary of the invention
For the above-mentioned prior art, for the present invention according to the physilogical characteristics of ruminant, providing one kind can be improved ruminant
The granular pattern probiotics of production performance, by yeast culture, aspergillus culture, lactic acid bacteria culture and bacillus subtilis
Compound compatibility is carried out, loss of activity and pulvis of the microbial forage additive in feed granulating can have both been well solved
The problem of type feed addictive and granular pattern feed cannot mix；Nutrition well can be provided again for animal and play Tiny ecosystem
The beneficial effect of preparation has effects that improve ruminant production performance.
To achieve the above object, the present invention adopts the following technical solutions:
The first aspect of the present invention provides a kind of granular pattern probiotics that ruminant production performance can be improved, by
25-35 parts of saccharomyces cerevisiae solid culture, 15-25 parts of aspergillus niger solid culture, 15-25 parts of aspergillus oryzae solid culture, cream
25-35 parts of sour bacterium solid culture and bacillus subtilis mixing granulation form；
In the granular pattern probiotics that ruminant production performance can be improved, the viable count of bacillus subtilis is not
Lower than 5 × 108cfu/g。
Preferably, the above-mentioned granular pattern probiotics that ruminant production performance can be improved, are by saccharomyces cerevisiae solid
30 parts of culture, 20 parts of aspergillus niger solid culture, 20 parts of aspergillus oryzae solid culture, 30 parts of lactic acid bacteria solid culture and withered
Careless bacillus mixing granulation forms.
Preferably, in the above-mentioned granular pattern probiotics that ruminant production performance can be improved, the bacillus subtilis
Bacterium is the bacillus subtilis Bacillus subtilis B7348 of deposit number CCTCC M 2010260.It can be with bacterium powder or hair
The form of zymotic fluid is added.
In the above-mentioned granular pattern probiotics that ruminant production performance can be improved, the saccharomyces cerevisiae solid culture
It is solid for saccharomyces cerevisiae (Saccharomyces cerevisiae) the BLNJ03 warp of CCTCC NO:M 2015209 by deposit number
Body fermenting and producing obtains.
The method of above-mentioned solid fermentation production are as follows: by saccharomyces cerevisiae (Saccharomyces cerevisiae) BLNJ03's
Liquid seeds are inoculated in solid fermentation culture medium by the inoculum concentration of 4%-6% (volume fraction), and fermentation temperature is 28-30 DEG C,
The aerobic fermentation time is 20-36h, and the anaerobic fermentation time is 72-96h.
The solid fermentation culture medium is by matrix and water by 1g:(0.5-0.7) mL forms；Wherein, the composition of matrix are as follows:
By mass percentage, wheat bran 90%, maize flour 3%, dregs of beans 7%.
In the above-mentioned granular pattern probiotics that ruminant production performance can be improved, the aspergillus niger solid culture is
It is raw through solid fermentation for aspergillus niger (Aspergillus niger) BLCC6-0009 of CCTCC NO:M 2015722 by deposit number
Production obtains.
The method of above-mentioned solid fermentation production are as follows: by the spore of aspergillus niger (Aspergillus niger) BLCC6-0009
It is inoculated in solid fermentation culture medium by the inoculum concentration of 0.01%-0.02% (mass fraction), fermentation temperature is 28-30 DEG C, is had
Aerobe fermentation 20-28h.
The solid fermentation culture medium is by matrix and water by 1g:(0.5-0.7) mL forms；Wherein, the group of matrix becomes
Wheat bran 100%.
In the above-mentioned granular pattern probiotics that ruminant production performance can be improved, the aspergillus oryzae solid culture is
It is sent out by aspergillus oryzae (Aspergillus oryzae) CICC 2174 (being purchased from Chinese industrial Culture Collection) through solid
Ferment produces to obtain.
The method of above-mentioned solid fermentation production are as follows: the spore of aspergillus oryzae (Aspergillus oryzae) CICC 2174 is pressed
The inoculum concentration of 0.01%-0.02% (mass fraction) is inoculated in solid fermentation culture medium, and fermentation temperature is 28-30 DEG C, aerobic
Ferment 20-28h.
The solid fermentation culture medium is by matrix and water by 1g:(0.5-0.7) mL forms；Wherein, matrix is wheat bran.
In the above-mentioned granular pattern probiotics that ruminant production performance can be improved, the lactic acid bacteria solid culture is
It is produced by the lactobacillus plantarum Lactobacillus plantarum that deposit number is CCTCC M 2010150 through solid fermentation
It arrives.
The method of above-mentioned solid fermentation production are as follows: by the freeze-drying of lactobacillus plantarum (Lactobacillus plantarum)
Powder seed is inoculated in solid fermentation culture medium by the inoculum concentration of 0.1%-0.2% (mass fraction), fermentation temperature 20-37
DEG C, 20~72h of anaerobic fermentation.
The solid fermentation culture medium is by matrix and water by 1g:(0.5-0.7) mL forms；Wherein, the composition of matrix are as follows:
By mass percentage, wheat bran 60%, powdered rice hulls 30%, DDGS (vinasse albumen feed) 10%.
The second aspect of the present invention provides the system of the above-mentioned granular pattern probiotics that ruminant production performance can be improved
Preparation Method, step are as follows: weigh various raw materials in proportion, all solids raw material is ground into can be by 20 meshes (0.85mm sieve pore)
Fine powder, stir evenly, 25kg~40kg/ ton of water be added, with 15 DEG C~50 DEG C of feeding stuff cuber granulations.
Granular pattern probiotics of the invention are the solid culture and withered grass gemma resistant to high temperature by a variety of probiotics
Bacillus compounds, not only nutrition rich in, but also its active constituent will not be because of the high temperature of granular pattern probiotics
Pelletization and lose；Wherein:
Yeast solids culture is the zymotechnique using liquid solid-state bond, that is, utilizes liquid strain, solid-state Quchi hair
Ferment, by what is obtained after drying process.A large amount of yeast metabolism products of its major function in product, in ruminant
Metabolic activity can be kept under ruminal environment and feed granulating Elevated Temperature Conditions, there are more than 100 kinds of metabolites in yeast culture,
Some be people more known to, such as aromatic substance, flavour enhancing substance, amino acid, oligosaccharides, organic acid, peptide etc., also some are seldom
The metabolic nutrition substance seen, is mainly used in ruminant and aquaculture, plays nutrition and health care double action.It is both domestic and external big
Quantity research proves, yeast culture promote growth of animal, improve efficiency of feed utilization, prevention disease, improve immunity of organisms and
Play a significant role in terms of improving environment.But the difference for preparing bacterial strain used in yeast solids culture will affect yeast culture
Using effect, since saccharomycete is a major class bacterium, the performance difference highly significant between different strains, the present invention to production ferment
Screening is optimized in the bacterial strain of female solid culture, in conjunction with the production performance of bacterial strain itself and matching between other bacterial strains
Cooperation is used, and determines the saccharomyces cerevisiae (Saccharomyces cerevisiae) for being CCTCC NO:M 2015209 with deposit number
Bacterial strain of the BLNJ03 as production yeast solids culture.The bacterial strain has been preserved in Wuhan, China city force on 04 08th, 2015
The China typical culture collection center (CCTCC) of Chinese university, being documented in another patent application of applicant, " one plant for solid
In the saccharomyces cerevisiae of body fermentation and its application " (CN105062905A).
Aspergillus oryzae and aspergillus niger are the bacterial strains for producing complex enzyme, can generate the protease, cellulase, starch of high stability
Enzyme, phytase etc., can tolerate higher temperature, and culture is to add in dedicated Zymolysis Equipment through strict control fermentation condition
Fungi preparation made from work, aspergillus oryzae culture and aspergillus niger culture are added in feed can pass through stimulation cellulose-decomposing bacterium
Growth improves the yield of cellulase, improves digestibility of fiber, stablizes ruminal pH value, improves ruminant tumor gastric function, thus
It improves ruminant production performance and improves body condition, reduces metabolic disease.Aspergillus culture bacterial strain uses therefor difference equally will affect
Its using effect needs to select strain excellent for the effect for reinforcing aspergillus culture.The aspergillus oryzae culture produced in the present invention
Bacterial strain uses therefor is aspergillus oryzae Aspergillus oryzae, which is purchased from Chinese industrial Culture Collection, bacterium
Kind number is CICC 2174；Production aspergillus niger culture bacterial strain uses therefor is aspergillus niger (Aspergillus niger) BLCC6-
0009, which is preserved in China typical culture collection center, deposit number CCTCC on December 04th, 2015
NO:M 2015722, another patent application for being documented in applicant " aspergillus niger of a plant height malaga carbohydrate oxidase and its are answered
With " in (CN105385609A).
Lactic acid bacteria is one of most commonly used probiotics in animal microecological formulation, is to be added in the form of viable bacteria, utilizes
The prebiotic effect of thallus, and the solid fermentation culture of lactic acid bacteria can produce a large amount of beneficial metabolic product, it is special such as to generate
Enzyme system, including generating the enzyme system of organic acid, synthesizing the enzyme system of polysaccharide, the enzyme system of decomposing lactic acid bacterium growth factor, decompose nitrous
The enzyme system of amine, the enzyme system for reducing cholesterol, the endotoxic enzyme system of control, lipolytic enzyme system, the enzyme for synthesizing various vitamins
System and the enzyme system etc. for decomposing cholic acid.These enzymes can not only accelerate the growth of lactic acid bacteria, and intestinal microecology can be maintained to balance；This
Outside, a large amount of organic acid, bacteriocin, hydrogen peroxide etc. also be can produce, pH value is adjusted to have, inhibit pathogenic bacteria growth, increase
The effect of strong immunity of organisms.For with regard to the lactic acid bacteria culture the effect of, select bacterial strain difference that can significantly affect its using effect,
It is lactobacillus plantarum Lactobacillus plantarum, the bacterium that lactic acid bacteria used in lactic acid bacteria culture is produced in the present invention
Strain was preserved in China typical culture collection center on 06 21st, 2010, and deposit number is CCTCC M 2010150,
It is documented in another patent of invention " one plant of lactic acid bacteria that can be used for biological preservation and its application " of applicant
(CN101914475B) in.
Bacillus subtilis is a kind of aerobic gram-positive bacteria that can form gemma, has resistance to temperature change, replies immediately fastly
Living and the features such as can produce a variety of enzyme activity, sporogenic form exists within producing bacillus subtilis product, to drying, high temperature, height
The resistance of the poor environments such as pressure, oxidation is very strong, can be resistant to the high temperature during feed granulating without suffering a loss, withered grass gemma
Bacillus enter after alimentary canal can the digestive enzymes such as the very strong protease of secretion activity, cellulase, lipase, amylase, these
Organized enzyme can directly improve the digestibility of nutritional ingredient in feed, reduce feedstuff-meat ratio, can be with indirect stimulation body enzyme itself
Activity, to improve the production performance of animal；In addition bacillus is aerobic bacteria, can be taken by force in oxygen support cud by biology and be detested
The growth of oxygen bacterium, maintains microecological balance in cud, reduces diarrhea and prevention disease, stimulates carbohydrate (forage) hair in cud
Ferment generates a large amount of volatile fatty acid, and wherein acetic acid and butyric acid content are most, and butyric acid and acetic acid are most in building butter fat
The primary raw material of number short chain fatty acids.The bacillus subtilis added in the present invention is bacterial strain Bacillus subtilis
B7348, the bacterial strain are preserved in China typical culture collection center, deposit number CCTCC on October 12nd, 2010
M 2010260, be documented in applicant another patent of invention " one plant of bacillus subtilis with stronger bacteriostasis and its
Using " in (CN102120975B).
The present inventor passes through in-depth study and creative labor, and optimized screening obtains from a large amount of test strain
The raw material prescription of granular pattern probiotics of the invention.Wherein, on the one hand consider different type bacterial strain and cultured products it
Between interaction relationship, on the other hand also contemplate in same class bacterium production performance and cultured products activity between different strains
Difference.In R&D process, by being combined to a large amount of different strains and its cultured products, obtained after research a combination thereof
The pellet performance of product formula, and the influence to ruminant production performance.As a result, it has been found that: with saccharomyces cerevisiae solid culture
Object, aspergillus niger solid culture, aspergillus oryzae solid culture, lactic acid bacteria solid culture and bacillus subtilis are combined,
Its pellet performance, the combination for being better than other different type bacterial strains and its cultured products to ruminant production performance.Herein
On the basis of, inventor is further respectively to saccharomyces cerevisiae, aspergillus niger, aspergillus oryzae, lactic acid bacteria and this kind of bacterium of bacillus subtilis
In different strains screening is optimized, due to the work of production performance and cultured products between the different strains of same class bacterium
Property also has differences, therefore, the performance of the product obtained after a combination thereof also significant difference.As a result, it has been found that: be by deposit number
25-35 parts of saccharomyces cerevisiae solid culture, the deposit number of CCTCC NO:M 2015209 is the black of CCTCC NO:M 2015722
15-25 parts of aspergillus solid culture, 15-25 parts of the aspergillus oryzae solid culture that bacterium numbering is CICC 2174, deposit number are
The withered grass bud that 25-35 parts of the lactobacillus plantarum solid culture of CCTCC M 2010150 and deposit number are CCTCC M 2010260
The viable count of spore bacillus, the granular pattern probiotics obtained after mixing granulation, enzyme activity and bacillus is before and after granulation
It there is no loss, reduce the loss of active constituent of the micro-ecological additive in pelletization to the greatest extent；Through dynamic
Object experimental verification, the granular pattern probiotics prepared with above-mentioned specific bacterium and its cultured products are to ruminant production performance
Improvement it is optimal.Raw material in product of the present invention prescription is deleted or replaced, effect obviously weakens；And in this base
Raw material is further added by plinth, unobvious to the improvement of product effect, even discovery overall effect is weakened during the test.
Therefore, the formula of the granular pattern probiotics that ruminant production performance can be improved of the invention is an organic whole,
It is not that conventional raw material replacement institute is getable.
Beneficial effects of the present invention:
(1) in the granular pattern probiotics that ruminant production performance can be improved of the invention, the training of saccharomyces cerevisiae solid
Supporting mutually coordinated can promote between object, aspergillus niger solid culture, aspergillus oryzae solid culture and lactic acid bacteria solid culture,
Its nutritional ingredient rich in can promote growth of animal, improve efficiency of feed utilization, improve ruminant tumor gastric function, from
And improve ruminant production performance and improve body condition, reduce metabolic disease；In conjunction with bacillus subtilis viable bacteria resistant to high temperature
Prebiotic effect, effectively maintain microecological balance in ruminant tumor gastric, reduce the generation of diarrhea.
(2) the granular pattern probiotics that ruminant production performance can be improved of the invention can both well solve micro-
Ecological feed additive loss of activity and powder-type feed addictive in feed granulating are asked with what granular pattern feed cannot mix
Topic, and good nutrition can be provided for animal and play the beneficial effect of probiotics, have and improves ruminant production
The effect of performance.
Specific embodiment
The present invention is further illustrated in conjunction with the embodiments, it should which explanation, following the description is merely to explain this
Invention, is not defined its content.
Embodiment 1: the preparation of the granular pattern probiotics of ruminant production performance can be improved
1. formula: 30 parts of saccharomyces cerevisiae solid culture, 20 parts of aspergillus niger solid culture, aspergillus oryzae solid culture 20
Part, 30 parts of lactic acid bacteria solid culture, bacillus subtilis powder.
Wherein, saccharomyces cerevisiae solid culture is the saccharomyces cerevisiae for being CCTCC NO:M 2015209 by deposit number
(Saccharomyces cerevisiae) BLNJ03 produces to obtain through solid fermentation；
The method of solid fermentation production are as follows: by the liquid of saccharomyces cerevisiae (Saccharomyces cerevisiae) BLNJ03
Seed is inoculated in solid fermentation culture medium by the inoculum concentration of 5% (volume fraction), and fermentation temperature is 30 DEG C, the aerobic fermentation time
For 30h, the anaerobic fermentation time is 80h.
The solid fermentation culture medium is made of matrix and water by 1g:0.6mL；Wherein, the composition of matrix are as follows: press quality
Percentages, wheat bran 90%, maize flour 3%, dregs of beans 7%.
Aspergillus niger solid culture is the aspergillus niger (Aspergillus for being CCTCC NO:M 2015722 by deposit number
Niger) BLCC6-0009 produces to obtain through solid fermentation.
The method of solid fermentation production are as follows: press the spore of aspergillus niger (Aspergillus niger) BLCC6-0009
The inoculum concentration of 0.01% (mass fraction) is inoculated in solid fermentation culture medium, and fermentation temperature is 30 DEG C, and aerobic fermentation is for 24 hours.
The solid fermentation culture medium is made of matrix and water by 1g:0.6mL；Wherein, the group of matrix becomes wheat bran
100%.
Aspergillus oryzae solid culture is passed through by the aspergillus oryzae (Aspergillus oryzae) that bacterium numbering is CICC 2174
Solid fermentation produces to obtain.
The method of solid fermentation production are as follows: press the spore of aspergillus oryzae (Aspergillus oryzae) CICC 2174
The inoculum concentration of 0.01% (mass fraction) is inoculated in solid fermentation culture medium, and fermentation temperature is 30 DEG C, and aerobic fermentation is for 24 hours.
The solid fermentation culture medium is made of matrix and water by 1g:0.6mL；Wherein, the group of matrix becomes wheat bran
100%.
Lactic acid bacteria solid culture is the lactobacillus plantarum Lactobacillus for being CCTCC M 2010150 by deposit number
Plantarum produces to obtain through solid fermentation；
The method of solid fermentation production are as follows: by the freeze-dried powder seed of lactobacillus plantarum Lactobacillus plantarum
It is inoculated in solid fermentation culture medium by the inoculum concentration of 0.2% (mass fraction), fermentation temperature is 30 DEG C, anaerobic fermentation 36h.
The solid fermentation culture medium is made of matrix and water by 1g:0.6mL；Wherein, the composition of matrix are as follows: press quality
Percentages, wheat bran 60%, powdered rice hulls 30%, DDGS 10%.
Bacillus subtilis powder is the bacillus subtilis Bacillus of deposit number CCTCC M 2010260
Subtilis B7348 is spray-dried after liquid fermentation and obtains.
The method of liquid fermentation production are as follows: by the liquid triangle of bacillus subtilis Bacillus subtilis B7348
Bottle seed is inoculated in seeding tank, and 37 DEG C of aerobic fermentation 15h are transferred in fermentor and put tank after 37 DEG C of aerobic fermentation 36h.
2. preparation method: all solids raw material, which is ground into, to be stirred by the fine powder of 20 meshes (0.85mm sieve pore)
Uniformly, 25kg~40kg/ tons of water (water of 25kg~40kg is added in raw material i.e. per ton) is added, is made with 25 DEG C of feeding stuff cuber
, the viable count of bacillus subtilis is 5.40 × 10 in the granular pattern probiotics of preparation8cfu/g。
Embodiment 2: the preparation of the granular pattern probiotics of ruminant production performance can be improved
Formula: 25 parts of saccharomyces cerevisiae solid culture, 25 parts of aspergillus niger solid culture, aspergillus oryzae solid culture 15
Part, 35 parts of lactic acid bacteria solid culture, bacillus subtilis powder.
Wherein, saccharomyces cerevisiae solid culture, aspergillus niger solid culture, aspergillus oryzae solid culture, lactic acid bacteria solid
The production method of culture and bacillus subtilis powder is the same as embodiment 1.
The preparation method is the same as that of Example 1, and the viable count of bacillus subtilis is 5.10 in the granular pattern probiotics of preparation
×108cfu/g。
Embodiment 3: the preparation of the granular pattern probiotics of ruminant production performance can be improved
Formula: 35 parts of saccharomyces cerevisiae solid culture, 15 parts of aspergillus niger solid culture, aspergillus oryzae solid culture 25
Part, 25 parts of lactic acid bacteria solid culture, bacillus subtilis powder.
Wherein, saccharomyces cerevisiae solid culture, aspergillus niger solid culture, aspergillus oryzae solid culture, lactic acid bacteria solid
The production method of culture and bacillus subtilis powder is the same as embodiment 1.
The preparation method is the same as that of Example 1, and the viable count of bacillus subtilis is 5.60 in the granular pattern probiotics of preparation
×108cfu/g。
Comparative example 1:
Formula: 30 parts of saccharomyces cerevisiae solid culture, 20 parts of aspergillus niger solid culture, aspergillus oryzae solid culture 20
Part, 30 parts of lactic acid bacteria solid culture, bacillus subtilis powder.
Wherein, saccharomyces cerevisiae solid culture is the feed saccharomyces cerevisiae for being CICC 1355 by deposit number
Saccharomyces cerevisiae produces to obtain through solid fermentation；
Aspergillus niger solid culture be by deposit number be CICC 40273 aspergillus niger Aspergillus niger through solid
Fermenting and producing obtains；
Aspergillus oryzae solid culture is solid for the aspergillus oryzae Aspergillus oryzae warp of CICC 41383 by deposit number
Body fermenting and producing obtains；
Lactic acid bacteria solid culture is the lactobacillus plantarum Lactobacillus for being CICC 21790 by deposit number
Plantarum produces to obtain through solid fermentation；
Bacillus subtilis powder is the bacillus subtilis Bacillus of deposit number CCTCC M 2010260
Subtilis B7348 is spray-dried after liquid fermentation and obtains.
Wherein, saccharomyces cerevisiae solid culture, aspergillus niger solid culture, aspergillus oryzae solid culture, lactic acid bacteria solid
The production method of culture and bacillus subtilis powder is the same as embodiment 1.
Preparation method with implementing 1, in the granular pattern probiotics of preparation the viable count of bacillus subtilis be 5.16 ×
108cfu/g。
Comparative example 2:
Formula: 30 parts of saccharomyces cerevisiae solid culture, 20 parts of aspergillus niger solid culture, aspergillus oryzae solid culture 20
Part, 30 parts of lactic acid bacteria solid culture, bacillus subtilis powder.
Wherein, saccharomyces cerevisiae solid culture is the feed saccharomyces cerevisiae for being CICC 1421 by deposit number
Saccharomyces cerevisiae produces to obtain through solid fermentation；
Aspergillus niger solid culture be by deposit number be CICC 40970 aspergillus niger Aspergillus niger through solid
Fermenting and producing obtains；
Aspergillus oryzae solid culture is solid for the aspergillus oryzae Aspergillus oryzae warp of CICC 40188 by deposit number
Body fermenting and producing obtains；
Lactic acid bacteria solid culture is the lactobacillus plantarum Lactobacillus for being CICC 21818 by deposit number
Plantarum produces to obtain through solid fermentation；
Bacillus subtilis is the bacillus subtilis Bacillus subtilis of deposit number CCTCC M 2010260
B7348。
Wherein, saccharomyces cerevisiae solid culture, aspergillus niger solid culture, aspergillus oryzae solid culture, lactic acid bacteria solid
The production method of culture and bacillus subtilis powder is the same as embodiment 1.
The preparation method is the same as that of Example 1, and the viable count of bacillus subtilis is 5.29 in the granular pattern probiotics of preparation
×108cfu/g。
Comparative example 3:
Formula: 30 parts of saccharomyces cerevisiae solid culture, 20 parts of aspergillus niger solid culture, aspergillus oryzae solid culture 20
Part, 30 parts of lactic acid bacteria solid culture, bacillus subtilis powder.
Wherein, saccharomyces cerevisiae solid culture is the feed saccharomyces cerevisiae for being CICC1562 by deposit number
Saccharomyces cerevisiae produces to obtain through solid fermentation；
Aspergillus niger solid culture be by deposit number be CICC 41125 aspergillus niger Aspergillus niger through solid
Fermenting and producing obtains；
Aspergillus oryzae solid culture is solid for the aspergillus oryzae Aspergillus oryzae warp of CICC 41478 by deposit number
Body fermenting and producing obtains；
Lactic acid bacteria solid culture is the lactobacillus plantarum Lactobacillus for being CICC 21822 by deposit number
Plantarum produces to obtain through solid fermentation；
Bacillus subtilis is the bacillus subtilis Bacillus subtilis of deposit number CCTCC M 2010260
B7348。
Wherein, saccharomyces cerevisiae solid culture, aspergillus niger solid culture, aspergillus oryzae solid culture, lactic acid bacteria solid
The production method of culture and bacillus subtilis powder is the same as embodiment 1.
The preparation method is the same as that of Example 1, and the viable count of bacillus subtilis is 5.33 in the granular pattern probiotics of preparation
×108cfu/g。
The variation of comparing embodiment 1 and each comparative example granulation front and back Enzyme activity, the results are shown in Table 1.
Each comparative example of table 1 and the granulation of embodiment 1 front and back Enzyme activity change
The display of table 1, the neutral protein that different saccharomyces cerevisiaes, aspergillus niger, aspergillus oryzae, lactic acid bacteria strains solid fermentation generate
Enzyme, amylase, cellulase, Xylanase activity are different, each equal highest of enzyme activity vigor of embodiment 1, and by granulation processing
Its each enzyme activity vigor does not lose substantially afterwards, and comparative example 1, comparative example 2, each enzyme activity vigor of comparative example 3 granulation processing after its
Loss late is 50% or more；Bacillus is since itself resistance is very strong, therefore viable count does not lose substantially before and after granulation.
Experiment results proved, the solid culture enzyme activity of each probiotics strain used in embodiment 1 and bacillus living number in granulation plus
It is not lost substantially before and after work, shows that its effective active composition is kept well, thereby may be ensured that it in animal productiong
In probiotic effects.
Compliance test result:
Application examples 1: the application effect test on mutton sheep
1 materials and methods
1.1 experimental animals and grouping
Test is carried out in Jining City in Shandong Province Jiaxiang County Small-fat-tail sheep cultivation base, and all fattening lambs use drylot feeding mode
Raising carries out under the conditions of the environmental conditions such as illumination ventilation are consistent.Select that body condition is good, weight is close to (20.45 ± 0.77)
Healthy fattening period Small-fat-tail sheep 360 without disease of kg, male and female is fifty-fifty, random by every group of fattening lamb 40 (+20 ♂ of 20 ♀)
It is divided into II group of control group, I group of test group and test, every group sets 3 repetitions.Control group fattening lamb feeds basal diet, examination
Test granular pattern probiotics prepared by the embodiment of the present invention 1 that I group is added 50g/ day on the basis of basal diet, examination
The competing product probiotics product that II group of feeding is bought in the market is tested, additive amount is 50g/ day.Test is selected with fattening lamb
Preliminary trial period 5d, formal test period 30d are set after fixed.Fattening lamb is freely eaten and drinking-water, other daily managements routinely carry out.
1.2 testing indexs and method
1.2.1 the forage volume of addition is recorded during growth performance test daily, daily early morning clears up hopper 1 time, weighs surplus
Clout weight calculates daily ingestion amount.On-test and the end same day weigh on an empty stomach in early morning, calculate average daily gain, averagely adopt day
Appetite and feed-weight ratio.
1.2.2 daily ration Nutrient Apparent Digestibility measured diet dry substance using full excrement method of receiving in positive examination the phase the 11st to 15 day
(DM), the nutrients such as organic matter (OM), thick protein (CP), neutral detergent fiber (NDF), acid detergent fiber (ADF) apparently disappear
Rate.
1.2.3 in early morning empty stomach jugular vein blood collection when the measurement off-test of Biochemical Indices In Serum, every group 4, every is taken out
It takes blood 10mL or so, 1500r/min to be centrifuged 15min, serum is taken to be sub-packed in 1.5mL centrifuge tube, is saved in -20 DEG C of refrigerators standby
With for measuring Biochemical Indices In Serum, including total serum protein (TP), albumin (ALB), urea nitrogen (BUN), total cholesterol
(TC), the content of triglycerides (TG) and blood glucose (Glu).
The measurement of TP content uses biuret colorimetric method, and the measurement of ALB content uses Bromocresol green, the survey of BUN content
Surely ultraviolet-glutamate dehydrogenase enzyme process is used, the measurement of TG, TC, Glu content uses enzymatic measurement.It is analyzed using full-automatic biochemical
Instrument (KHB brilliance 330, Shanghai Kehua Bio-engineering Co., Ltd) is measured, and kit is purchased from China, Shanghai section biology
Engineering stock Co., Ltd.
1.3 statistical analysis test datas are handled through Excel, are carried out using 16.0 statistical software One-Way ANOVA of SPSS
Variance analysis, LSD method carry out group difference significance test, and test data is indicated with mean+SD, with the work of P < 0.05
For significant difference judgment criteria.
2 results and analysis
The influence of 2.1 pairs of growth performances
As shown in Table 2, it tests I group and tests II group of average daily gain and increase separately 12.88% and 7.36% compared with control group,
It wherein tests I group of average daily gain index to be significantly higher than control group (P < 0.05), tests II group of average daily gain and control group
Comparing difference is not significant；The average daily gain for testing I group and II group of test increases separately 5.52% and 4.29% compared with control group,
Equal difference is not significant (P > 0.05).Testing I group and testing II group of feed-weight ratio reduces by 6.50% and 2.83% compared with control group respectively,
Significant difference (P < 0.05).It tests I group of average daily gain and is higher than II group of test, while the feed-weight ratio for testing I group is substantially less than
II group is tested, I group of test is substantially less than control group with the feed-weight ratio for testing II group.It is real that the present invention is added in daily ration as the result is shown
The granular pattern probiotics for applying the preparation of example 1 can improve the daily gain of fattening lamb to a certain extent, reduce its feed-weight ratio, show
Granular pattern probiotics prepared by the embodiment of the present invention 1 have certain facilitation to the growth performance of fattening lamb, and imitate
Fruit is better than competing product group.
Influence of the table 2 to fattening lamb growth performance
Note: colleague data shoulder mark difference capitalization person indicates that difference is extremely significant (P < 0.01)；Shoulder mark difference lowercase
It indicates significant difference (P < 0.05)；Shoulder mark same letter indicates that difference is not significant (P > 0.05) without letter.Similarly hereinafter.
The influence of 2.2 pairs of daily ration Nutrient Apparent Digestibilities
As shown in Table 3, after testing 30 days, I, II group of the apparent digestibility test of OM, NDF are extremely significant to be higher than control group (P
< 0.01)；I group of the apparent digestibility test of DM is extremely significant to be higher than control group (P < 0.01), and II group of test is significantly higher than control group
(P < 0.05), I group of test is higher than II group of test；The apparent digestibility of CP tests I, II group and is all remarkably higher than control group (P <
0.05), while I group of test is significantly higher than II group (P < 0.05) of test；I group of ADF apparent digestibility test is extremely significant to be higher than control
Group (P < 0.01), test II group with test I group, control group compared to equal difference not significantly (P > 0.05).
Table 3 to daily ration nutrient fattening lamb all-digestive tract apparent digestibility influence
The influence of 2.3 pairs of Biochemical Indices In Serums
As shown in Table 4, after testing 30d, the serum Glu content of I, II group of test is all remarkably higher than control group (P < 0.05),
And the serum Glu content of I group of test is higher than II group of test；The BUN content for testing I, II group of serum is substantially less than control group (P
< 0.05), and I group of serum BUN content is tested lower than II group of test；Each test group of TC, TG, TP, ALB content is compared and is respectively tried
Testing group, equal difference is not significant compared with the control group, but tests I group and II group of TP and ALB content of test has compared with control group and increases
Trend.
Influence of the table 4 to fattening lamb Biochemical Indices In Serum
3 discuss
The influence probiotics of 3.1 pairs of growth performances are to cultivate probiotics through artificial screening, then through modern biotechnology work
Journey the factorial production, dedicated for the product of animal nutritious health, daily ration palatability can be improved by making an addition in livestock and poultry diet, be improved
Daily ration conversion ratio enhances the immunity and premunition of animal body.The group average daily gain of this test results test is significantly higher than pair
According to group, feed-weight ratio is substantially less than control group, due to the particles used type probiotics of this test, containing a large amount of probiotics and disappears
Change the metabolites such as enzyme, is applied to fattening lamb and produces, its daily gain can be improved, feed-weight ratio is reduced, to improve growth performance.
The granular pattern for influencing the addition embodiment of the present invention 1 preparation in this test of 3.2 pairs of feed digestion absorption rates is micro-
The test group of ecological agent extremely significant can improve daily ration DM, OM, NDF and ADF in the apparent digestibility of fattening lamb all-digestive tract
(P < 0.01) significantly improves daily ration CP at fattening lamb all-digestive tract apparent digestibility (P < 0.05), to be conducive to improve
Its production performance.
The protein utilization rate for influencing serum urea nitrogen and daily ration of 3.3 pairs of Biochemical Indices In Serums it is related (Stanley etc.,
2002), correlation analysis the result shows that, urea nitrogen content and internal nitrogen retention rate, protein or rate of ultilization of amino acid are negatively correlated
(Dong Zhiyan etc., 2010).In this test, the serum urea nitrogen content of test group is substantially less than control group (P < 0.05), and daily ration
CP digestibility significantly improve.It is reported that 6.1mmol/L is not to be exceeded in animal blood glucose concentration, the high yield in normal concentration range
Animal blood glucose content be higher than low yield animal (Li Jianguo etc., 2006；Stanley etc., 2002), this test result also demonstrates this
The blood-sugar content of point, test group is apparently higher than control group, can preferably meet the needs that body synthesizes glucose, to be conducive to
The growth of fattening lamb.This test each group total serum protein, albumin, cholesterol, the equal difference of content of triglycerides is not significant,
But test group total serum protein and albumin have the tendency that increasing compared with control group, this is almost the same with the performance of growth performance.
4 conclusions
Granular pattern probiotics prepared by the present invention are added in daily ration can reduce the serum urea nitrogen content of fattening lamb,
Blood sugar concentration is improved in normal concentration range, improves but do not influence total protein and albumin content in serum, is improved in daily ration
The apparent digestibility of DM, OM, CP, NDF, ADF improve daily gain, reduce feed-weight ratio, improve growth performance, and indices are equal
Better than market competition product.
Application examples 2: the application effect test on milk cow
1 materials and methods
1.1 experimental animals and grouping
Test carries out in Feicheng, Shandong Province Ya Aote Cow product base.Test choose the age, parity, lactation number of days,
The output of milk is similar to be produced lactating cow 45, is randomly divided into II group, every group 15 of control group, I group of test group and test.Control
Group feeding basal diet is tested the embodiment of the present invention 1 that I group is added 500g/ (previous day) on the basis of basal diet and is prepared
Granular pattern probiotics, the competing product probiotics product bought in the market of II group of feeding of test, additive amount 500g/
(previous day), milk cow day feed twice, and day milks 2 times.Test sets preliminary trial period 7 days, and formal test period 15 days, the daily management of milk cow
According to conventional progress.
1.2 testing index
Daily morning and dusk record the output of milk of every group of every cow head during test, calculate test group and control group is average
The output of milk of daily every cow head, every 7 days outputs of milk, which are done, once summarizes comparative analysis.
2 test results
The influence of 2.1 pairs of outputs of milk
5 output of milk test result of table
By table 5 as it can be seen that every cow head can increase daily after granular pattern probiotics prepared by the feeding embodiment of the present invention 1
Add 7% or more the output of milk (2% or more concurrent control group decline), the same period every cow head of market competition product can increase milk production daily
4% or more amount (2% or more concurrent control group decline).Test result confirms granular pattern Tiny ecosystem prepared by the embodiment of the present invention 1
Effect of increasing production of the preparation on milk cow is better than market competition product.
Application examples 3: the application effect test on beef cattle
1 materials and methods
1.1 experimental animals and grouping
Test is carried out in Tai'an theatre beef cattle, and the nearly 180kg of average weight, the wean in 6~August age is selected to hybridize bull
36, it is randomly divided into II group, every group 12 of control group, I group of test group and test.Control group fed basal diet, test I group
Granular pattern probiotics prepared by the embodiment of the present invention 1 on 200g/ (previous day), test II are added on the basis of basal diet
The competing product probiotics product that group feeding is bought in the market, additive amount are 200g/ (previous day).Each group ox is only every during test
The smart feed supplement of its feeding phase homogenous quantities and roughage, experimental period 45 days.
1.2 testing index
Each group ox weight is weighed before test and after test, calculates the daily gain index of beef cattle.
2 test results
Influence of the table 6 to beef cattle production performance indicator
By table 6 as it can be seen that granular pattern probiotics group beef cattle prepared by the feeding embodiment of the present invention 1 is increased production compared with control group
On the 0.11kg/ previous day, compared with control group volume increase 13.92%, competing product group beef cattle increases production the 0.06kg/ previous day compared with control group, relatively compares
Volume increase 7.59%, the more competing product group of granular pattern probiotics group beef cattle prepared by the feeding embodiment of the present invention 1 increase production 0.05kg/
The previous day, volume increase 5.88%.
Claims (6)
1. a kind of granular pattern probiotics that ruminant production performance can be improved, which is characterized in that by deposit number be CCTCC
25-35 parts of the saccharomyces cerevisiae solid culture of NO:M 2015209, the aspergillus niger that deposit number is CCTCC NO:M 2015722 are solid
15-25 parts of body culture, 15-25 parts of the aspergillus oryzae solid culture that bacterium numbering is CICC 2174, deposit number are CCTCC M
The bacillus subtilis that 25-35 parts of 2010150 lactobacillus plantarum solid culture and deposit number are CCTCC M 2010260 is mixed
Conjunction is pelletized；
Wherein, the production method of saccharomyces cerevisiae solid culture are as follows: the wine brewing ferment for being CCTCC NO:M 2015209 by deposit number
Female liquid seeds are inoculated in solid fermentation culture medium, and fermentation temperature is 28-30 DEG C, and the aerobic fermentation time is 20-36h, are detested
The aerobe fermentation time is 72-96h；
The production method of aspergillus niger solid culture are as follows: connect the spore for the aspergillus niger that deposit number is CCTCC NO:M 2015722
Kind is in solid fermentation culture medium, and fermentation temperature is 28-30 DEG C, aerobic fermentation 20-28h；
The production method of aspergillus oryzae solid culture are as follows: the aspergillus oryzae that bacterium numbering is CICC 2174 is inoculated in solid fermentation
In culture medium, fermentation temperature is 28-30 DEG C, aerobic fermentation 20-28h；
The production method of lactobacillus plantarum solid culture are as follows: the lactobacillus plantarum for being CCTCC M 2010150 by deposit number
Freeze-dried powder seed is inoculated in solid fermentation culture medium, and fermentation temperature is 20-37 DEG C, 20~72h of anaerobic fermentation.
2. the granular pattern probiotics of ruminant production performance can be improved as described in claim 1, which is characterized in that by
30 parts of saccharomyces cerevisiae solid culture that deposit number is CCTCC NO:M 2015209, deposit number are CCTCC NO:M 2015722
20 parts of aspergillus niger solid culture, 20 parts of the aspergillus oryzae solid culture that bacterium numbering is CICC 2174, deposit number be
The withered grass gemma that 30 parts of the lactobacillus plantarum solid culture of CCTCC M 2010150 and deposit number are CCTCC M 2010260
Bacillus mixing granulation forms.
3. the granular pattern probiotics of ruminant production performance can be improved as claimed in claim 1 or 2, feature exists
In in the probiotics, the viable count of bacillus subtilis is not less than 5 × 108cfu/g。
4. the granular pattern probiotics of ruminant production performance can be improved as described in claim 1, which is characterized in that institute
Solid fermentation culture medium is stated by matrix and water by 1g:(0.5-0.7) mL forms；Wherein, the composition of matrix are as follows: press quality percentage
Than meter, wheat bran 90%, maize flour 3%, dregs of beans 7%.
5. the system of the granular pattern probiotics described in any one of Claims 1-4 that ruminant production performance can be improved
Preparation Method, which is characterized in that step are as follows: solid material is crushed, is stirred evenly, the water of 25kg~40kg/ tons of addition, 15 DEG C~
50 DEG C granulation to get.
6. the feeding that granular pattern probiotics of any of claims 1-4 improve ruminant production performance in preparation
Application in material.
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