CN106076442B - Centrifugation bottle for PRP multistage parallel extraction, and use method thereof - Google Patents
Centrifugation bottle for PRP multistage parallel extraction, and use method thereof Download PDFInfo
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- CN106076442B CN106076442B CN201610399268.6A CN201610399268A CN106076442B CN 106076442 B CN106076442 B CN 106076442B CN 201610399268 A CN201610399268 A CN 201610399268A CN 106076442 B CN106076442 B CN 106076442B
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0631—Purification arrangements, e.g. solid phase extraction [SPE]
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- Health & Medical Sciences (AREA)
- Clinical Laboratory Science (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- External Artificial Organs (AREA)
- Centrifugal Separators (AREA)
Abstract
The present invention provides a centrifugation bottle for PRP multistage parallel extraction, and a use method thereof. The centrifugation bottle comprises a sealed separation layer arranged inside the centrifugation bottle body, wherein the centrifugation bottle cavity of the centrifugation bottle is separated into a sealed first chamber and a sealed second chamber through the sealed separation layer, the first chamber and the second chamber are communicated through a U-shaped pipe, the bottle cover position of the first chamber is provided with a first air port, a first conducting pipe and a blood plasma injection port, and the bottle cover position of the second chamber is provided with a second air port and a second conducting pipe. With the centrifugation bottle of the present invention, the blood plasma can be subjected to multistage centrifugation, the transferring of the blood is not required, the pollution is not generated, and the purity is high.
Description
Technical field
The present embodiments relate to technical field of medical instruments, it is more particularly to a kind of for PRP multistage parallels extract from
Heart bottle and its using method.
Background technology
Platelet rich plasma (Platelet Rich Plasma, abbreviation PRP) is by containing height obtained from centrifugal blood
The thrombocyte plasma of concentration.The platelet of human body also contains in addition to it can provide cohesion when hemostasis in platelet
Have much relevant with a wound healing and bone regeneration various somatomedin, such as platelet derived growth factor, conversion growth because
Son, insulin like growth factor, epidermal growth factor and VEGF etc..Somatomedin in platelet,
Differentiation and propagation to cell plays facilitation, and these somatomedin have each other cooperative response, thin with other promotions
The factor interaction of cytoactive and impact, maintain the balance of organizational environment jointly, have to wound healing, reparation and regeneration
Important effect.Further, since PRP can be extracted from patient's self-blood, after extracted enrichment is processed, can be used for related disease
The treatment of disease, thus abundance, draw materials conveniently, and preparation method is simple and body can be allowed to absorb.Itself PRP is using can keep away
The problem for exempting from virus disseminating and immunologic rejection occurs.Therefore PRP is widely applied in different medical domains.Such as orthopaedics, mouth
Chamber section, decorative sursery, sports medical science and aesthetic medicine.
At present, PRP prepares the centrifuge bottle included with suit for carrying out centrifugally operated to blood.But current centrifugation
There is problems with bottle：1. outside contamination is easily subject to, is shifted in multiple containers, increased platelet activation and contaminated machine
Meeting；2. PRP collections purity is not high, inefficiency；3. easily affected by operation factors, preparation method is not sufficiently stable.
The content of the invention
To solve above-mentioned problem, the present invention provides a kind of centrifuge bottle extracted for PRP multistage parallels and its makes
With method, it is possible to achieve efficiently quickly PRP is extracted, and the PRP purity extracted is higher.
The present invention provides a kind of centrifuge bottle extracted for platelet rich plasma PRP multistage parallels, wherein：In centrifuge bottle sheet
It is provided with closed interlayer in vivo, centrifuge bottle cavity is divided into the closed interlayer first cavity and the second cavity of sealing, described
One cavity and the second cavity are connected by U-tube；Be provided with bottle cap on first cavity the first air scoop, the first conduit and
Blood plasma inlet；The second air scoop and the second conduit are provided with bottle cap on second cavity.
Further, the bottom of the centrifugation bottle body is coniform, the lower port of first conduit and the second conduit
And the two-port of U-tube is located at the cone shape bottom.
Further, first air scoop, the first conduit, blood plasma inlet, U-tube, the second air scoop, the second conduit
Sealing rubber ring is equipped with bottle cap junction.
Further, it is hose connection to be located at the centrifuge bottle body exterior in the U-tube, and in hose switch is provided with
Control device.
Further, first air scoop, the first conduit, blood plasma inlet, the second air scoop and the second conduit from
The outside of heart bottle body is equipped with closure.
Further, the interlayer is V-type, and centrifuge bottle cavity is divided into into first cavity and the second cavity of sealing, described
Volume of the volume of the first cavity more than second cavity.
The present invention also provides a kind of using method of the centrifuge bottle extracted using above-mentioned any one PRP multistage parallel, bag
Include：
Step one：The first air scoop is opened, will be injected in centrifuge bottle by blood plasma inlet containing anticoagulant fresh blood
The first cavity in, then by the first air scoop, the first conduit and blood plasma inlet seal, the centrifuge bottle is placed in into centrifuge
In be centrifuged；
Step 2：After the completion of centrifugation, the first air scoop is opened, extracted in the first chamber by the first conduit using syringe
In vivo the red blood cell layer of bottom, when syringe is extracted to red blood cell layer and the PRP layers demarcation line lower limb in intermediate layer, stops
Only extract, by the sealing of the first conduit mouth；
Step 3：The air of second cavity is outwards extracted by the second air scoop using syringe so that the first chamber
In vivo the PRP layers of bottom are acted on by air pressure, are input in second cavity by U-tube, when being drawn in the first cavity
PRP layers and supernatant layer demarcation line top edge when, stop extracting；
Step 4：First air scoop and the second air scoop are sealed, the centrifuge bottle is placed in a centrifuge to be carried out again
Centrifugation；
Step 5：After the completion of recentrifuge, the first air scoop and the second air scoop are opened, with syringe the second conduit is passed through
The second cavity inner bottom part red blood cell layer is extracted, when syringe is extracted to red blood cell layer and PRP layer demarcation line, new injection is exchanged for
Device extracts the PRP layers collected in the second cavity by the second conduit.
Further, also include between step 3 and step 4：First conduit is passed through by the first cavity using syringe
Interior supernatant layer is extracted, and then contains anticoagulant fresh blood to the injection of the first cavity again by blood plasma inlet.
Further, in PRP layers in the second cavity after collecting centrifugation, can extract not commensurability according to actual needs
Clear liquid, be mixedly configured into variable concentrations PRP.
The PRP centrifuge bottles provided using the present invention, with advantages below：
(1) secondary centrifuging can be carried out to blood plasma, and when secondary centrifuging is carried out, without the need for blood is transferred to outside centrifuge bottle
Portion, is directly shifted inside device, will not be contaminated；
(2) it is that centrifugal liquid is stored in smaller container when second is centrifuged, the boundary of PRP layers and red blood cell layer
Line is obvious, the higher rich platelet blood of easy DNA purity；
(3) can realize that parallel multi-level is extracted so that same centrifuge bottle, realize that multistage parallel is extracted, lift
Efficiency.
Description of the drawings
In order to be illustrated more clearly that the embodiment of the present invention or technical scheme of the prior art, below will be to embodiment or existing
The accompanying drawing to be used needed for having technology description is briefly described, it should be apparent that, drawings in the following description are these
Some bright embodiments, for those of ordinary skill in the art, without having to pay creative labor, can be with
Other accompanying drawings are obtained according to these accompanying drawings.
Fig. 1 is the structural representation of the centrifuge bottle embodiment one extracted provided by the present invention for PRP multistage parallels；
Fig. 2 is the structural representation of the bottle cap embodiment one in Fig. 1；
Fig. 3 is the structural representation of the bottle cap embodiment two in Fig. 1；
Fig. 4 is the centrifuge bottle using effect figure extracted provided by the present invention for PRP multistage parallels.
Reference：
The closed interlayer 3U type pipes of 1 centrifuge bottle 2
The conduit of 10 first cavity, 11 first air scoop 12 first
The air scoop of 13 blood plasma inlet, 20 second cavity 21 second
22 second conduits
Specific embodiment
To make purpose, technical scheme and the advantage of the embodiment of the present invention clearer, below in conjunction with the embodiment of the present invention
In accompanying drawing, the technical scheme in the embodiment of the present invention is clearly and completely described, it is clear that described embodiment is
The a part of embodiment of the present invention, rather than the embodiment of whole.Based on the embodiment in the present invention, those of ordinary skill in the art
The every other embodiment obtained under the premise of creative work is not made, belongs to the scope of protection of the invention.
The embodiment of the present invention provides a kind of centrifuge bottle extracted for platelet rich plasma PRP multistage parallels, such as Fig. 1~figure
Shown in 2, closed interlayer 2 is provided with centrifugation bottle body 1, centrifuge bottle cavity is divided into the closed interlayer 2 first chamber of sealing
The cavity 20 (outside of two cavitys can be according to using labelling high scale) of body 10 and second, the cavity of first cavity 10 and second
20 are connected by U-tube 3；The first air scoop 11, the first conduit 12 and blood plasma note is provided with bottle cap on first cavity 10
Entrance 13；The second air scoop 21 and the second conduit 22 are provided with bottle cap on second cavity 20.
In said structure, for the ease of collecting the liquid of bottom, it is preferable that the bottom of the centrifugation bottle body 1 is circular cone
Shape, the lower port of the conduit 22 of first conduit 12 and second and the two-port of U-tube 3 are located at the cone shape bottom.
In said structure, in order to increase sealing, it is preferable that first air scoop 11, the injection of the first conduit 12, blood plasma
Mouth 13, U-tube 3, the second air scoop 21, the second conduit 22 are equipped with sealing rubber ring in bottle cap junction.And it is described first empty
QI KOU 11, the first conduit 12, blood plasma inlet 13, the second air scoop 21 and the second conduit 22 are equal in the outside of centrifugation bottle body 1
It is provided with closure.
When extracting PRP using said structure, specific implementation step can include：
Step one：The first air scoop 11 is opened, centrifugation will be injected by blood plasma inlet 13 containing anticoagulant fresh blood
Bottle 1 in the first cavity 10 in, then the first air scoop 11, the first conduit 12 and blood plasma inlet 13 are sealed, by it is described from
Heart bottle 1 is placed in a centrifuge and is centrifuged；
Step 2：After the completion of centrifugation, blood plasma can be divided into three layers, i.e. supernatant layer, PRP layers and red blood cell layer.Now, open
First air scoop 11, using syringe the red blood cell layer of the bottom in the first cavity 10 is extracted by the first conduit 12, works as note
(purpose is to retain red blood cell layer and PRP when emitter is extracted to red blood cell layer and the PRP layers demarcation line lower limb in intermediate layer
Mixed liquor between layer), stop extracting, by the 12 mouthfuls of sealings of the first conduit；
Step 3：The air of second cavity 20 is outwards extracted by the second air scoop 21 using syringe.Now
One air scoop 11 is open mode, and the second air scoop 21 is sealing state, then can exist in the first cavity 10 and the second cavity 20
Draught head, so that the PRP layers of bottom are acted on by air pressure in the first cavity 10, by U-tube 3 second chamber is input to
In body 20, (PRP layers and supernatant are retained when the PRP layers being drawn in the first cavity 10 are with supernatant layer demarcation line top edge
Layer boundary mixing liquid), stop extracting；
Step 4：First air scoop 11 and the second air scoop 21 are sealed, the centrifuge bottle 1 is placed in a centrifuge to be carried out
Recentrifuge；
Step 5：After the completion of recentrifuge, the first air scoop 11 and the second air scoop 21 are opened, with syringe second is passed through
Conduit 22 extracts the inner bottom part red blood cell layer of the second cavity 20, when syringe is extracted to red blood cell layer and PRP layer demarcation line, exchanges for
New syringe extracts the PRP layers collected in the second cavity 20 by the second conduit 22.
Now, the PRP purity collected from the second cavity 20 is just very high, reason be on the one hand the second cavity 20 relative to
Overall centrifuge bottle, between scale value it is more accurate, be easier to distinguish the boundary between three layers produced after recentrifuge, the
What two aspects were centrifuged due to the second cavity 20 is exactly to pass through the PRP liquid being once centrifuged, on this basis recentrifuge
Operation, the purity for lifting will be higher.
For the ease of control U-tube 3, further, can also in the specific implementation, the U-tube 3 be located at it is described from
The outside of heart bottle body 1 is hose connection, and in hose switch controlling device is provided with.Herein switch controlling device does not do concrete limit
It is fixed, as long as energy closed flexible pipe so that U-tube 3 is not turned on.
In said method, in order to concurrent working can be realized, also include between step 3 and step 4：Using syringe
The supernatant layer in first cavity 10 is extracted by the first conduit 12, then again by blood plasma inlet 13 to the first cavity
10 injections contain anticoagulant fresh blood.Now, recentrifuge so that the first cavity 10 and the second cavity 20 it is simultaneously parallel from
The heart, design sketch as shown in figure 4, the first cavity 10 centrifugation be for extract primitive plasma, the second cavity 20 centrifugation be
PRP liquid after first time centrifugally operated.
Due to the PRP amounts contained in blood it is less, further in order to increase the efficiency of extraction, in structure design, institute
It can be V-type to state interlayer, concrete as shown in figure 3, centrifuge bottle cavity is divided into into first cavity 10 and the second cavity 20 of sealing, institute
State the volume of the volume more than second cavity 20 of the first cavity 10.Such structure can be caused as far as possible in the first chamber
Body 10 injects more primitive plasmas so that the PRP amounts of extraction are larger purer.
Additionally, in PRP layers in the second cavity 20 after collecting centrifugation, can extract different amounts of according to actual needs
Clear liquid, is mixedly configured into variable concentrations PRP.
The PRP centrifuge bottles provided using the present invention and using method, with pollution-free, purity is high, efficiency high, extracted amount is big
Advantage.
Although more herein used the terms such as such as centrifuge bottle, blood plasma inlet, conduit mouth, air scoop, and
It is not excluded for using the probability of other terms.It is used for the purpose of more easily describing and explaining the sheet of the present invention using these terms
Matter；It is all contrary with spirit of the invention to be construed as any additional restriction.
Finally it should be noted that：Various embodiments above only to illustrate technical scheme, rather than a limitation；To the greatest extent
Pipe has been described in detail with reference to foregoing embodiments to the present invention, it will be understood by those within the art that：Its according to
So the technical scheme described in foregoing embodiments can be modified, either which part or all technical characteristic are entered
Row equivalent；And these modifications or replacement, do not make the essence disengaging various embodiments of the present invention technology of appropriate technical solution
The scope of scheme.
Claims (9)
1. it is a kind of for platelet rich plasma PRP multistage parallels extract centrifuge bottle, it is characterised in that：In centrifugation bottle body (1)
Closed interlayer (2) is inside provided with, centrifuge bottle cavity is divided into the closed interlayer (2) first cavity (10) and the second cavity of sealing
(20), first cavity (10) is connected with the second cavity (20) by U-tube (3)；Bottle cap on first cavity (10)
Place is provided with the first air scoop (11), the first conduit (12) and blood plasma inlet (13)；At bottle cap on second cavity (20)
It is provided with the second air scoop (21) and the second conduit (22).
2. the centrifuge bottle that PRP multistage parallels according to claim 1 are extracted, it is characterised in that：Centrifugation bottle body (1)
Bottom for coniform, the two-port of the lower port and U-tube (3) of first conduit (12) and the second conduit (22) is located at
The cone shape bottom.
3. the centrifuge bottle that PRP multistage parallels according to claim 1 are extracted, it is characterised in that：First air scoop
(11), the first conduit (12), blood plasma inlet (13), U-tube (3), the second air scoop (21), the second conduit (22) are tied in bottle cap
Sealing rubber ring is equipped with conjunction.
4. the centrifuge bottle that PRP multistage parallels according to claim 1 are extracted, it is characterised in that：In the U-tube (3) position
It is hose connection outside centrifugation bottle body (1), in hose switch controlling device is provided with.
5. the centrifuge bottle that PRP multistage parallels according to claim 1 are extracted, it is characterised in that：First air scoop
(11), the first conduit (12), blood plasma inlet (13), the second air scoop (21) and the second conduit (22) are in centrifugation bottle body (1)
Outside be equipped with closure.
6. the centrifuge bottle that the PRP multistage parallels according to any one of Claims 1 to 5 are extracted, it is characterised in that：It is described every
Layer is V-type, and centrifuge bottle cavity is divided into into first cavity (10) and the second cavity (20) of sealing, the appearance of first cavity (10)
Volume of the product more than second cavity (20).
7. the using method of the centrifuge bottle that a kind of PRP multistage parallels any one of employing claim 1~6 are extracted, its
It is characterised by, including：
Step one：The first air scoop (11) is opened, will be containing anticoagulant fresh blood by blood plasma inlet (13) injection centrifugation
It is then that the first air scoop (11), the first conduit (12) and blood plasma inlet (13) is close in the first cavity (10) in bottle (1)
Envelope, the centrifuge bottle (1) is placed in a centrifuge and is centrifuged；
Step 2：After the completion of centrifugation, the first air scoop (11) is opened, extracted in the by the first conduit (12) using syringe
The red blood cell layer of the interior bottom of one cavity (10), when syringe is extracted to red blood cell layer and under the PRP layers demarcation line in intermediate layer
During edge, stop extracting, by the sealing of the first conduit (12) mouth；
Step 3：The air of second cavity (20) is outwards extracted by the second air scoop (21) using syringe so that the
The PRP layers of the interior bottom of one cavity (10) are acted on by air pressure, are input in second cavity (20) by U-tube (3), when taking out
When getting the PRP layers in the first cavity (10) with supernatant layer demarcation line top edge, stop extracting；
Step 4：First air scoop (11) and the second air scoop (21) are sealed, by the centrifuge bottle (1) be placed in a centrifuge into
Row recentrifuge；
Step 5：After the completion of recentrifuge, the first air scoop (11) and the second air scoop (21) are opened, with syringe second is passed through
Conduit (22) extracts the second cavity (20) inner bottom part red blood cell layer, when syringe is extracted to red blood cell layer and PRP layer demarcation line,
Exchange new syringe for and the PRP layers collected in the second cavity (20) are extracted by the second conduit (22).
8. the using method of the centrifuge bottle that PRP multistage parallels according to claim 7 are extracted, it is characterised in that in step
Also include between three and step 4：Using syringe the supernatant layer in the first cavity (10) is taken out by the first conduit (12)
Remove, then contain anticoagulant fresh blood to the first cavity (10) injection again by blood plasma inlet (13).
9. the using method of the centrifuge bottle that PRP multistage parallels according to claim 7 are extracted, it is characterised in that：Collecting
After centrifugation during PRP layers in the second cavity (20), according to actual needs different amounts of clear liquid can be extracted, be mixedly configured into not
With concentration PRP.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610399268.6A CN106076442B (en) | 2016-06-07 | 2016-06-07 | Centrifugation bottle for PRP multistage parallel extraction, and use method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610399268.6A CN106076442B (en) | 2016-06-07 | 2016-06-07 | Centrifugation bottle for PRP multistage parallel extraction, and use method thereof |
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| Publication Number | Publication Date |
|---|---|
| CN106076442A CN106076442A (en) | 2016-11-09 |
| CN106076442B true CN106076442B (en) | 2017-05-17 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201610399268.6A Active CN106076442B (en) | 2016-06-07 | 2016-06-07 | Centrifugation bottle for PRP multistage parallel extraction, and use method thereof |
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Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112934287A (en) * | 2021-02-26 | 2021-06-11 | 深圳市金灿华实业有限公司 | Centrifugal tube and PRP extraction method using same |
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|---|---|---|---|---|
| CN1058551A (en) * | 1990-08-03 | 1992-02-12 | 让·吉根 | Device with blood plasma in the centrifugal separation separating whole blood |
| CN1583213A (en) * | 2004-06-11 | 2005-02-23 | 西安瑞捷生物医疗技术有限公司 | Apparatus for preparing thrombocyte enriched blood plasma and preparing method thereof |
| CN1658941A (en) * | 2002-04-08 | 2005-08-24 | 热起源公司 | Blood component separation method and apparatus |
| CN103974777A (en) * | 2011-11-01 | 2014-08-06 | 朴铉贞 | Container for centrifugal separation providing rapid centrifugal separation |
| CN104694382A (en) * | 2015-03-09 | 2015-06-10 | 余家昌 | Rapid nucleic acid extraction device and method |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1261183A (en) * | 2000-02-21 | 2000-07-26 | 华南理工大学 | Management information automatization system and its autocontrol method and program for passenger traffic stations on highway |
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2016
- 2016-06-07 CN CN201610399268.6A patent/CN106076442B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1058551A (en) * | 1990-08-03 | 1992-02-12 | 让·吉根 | Device with blood plasma in the centrifugal separation separating whole blood |
| CN1658941A (en) * | 2002-04-08 | 2005-08-24 | 热起源公司 | Blood component separation method and apparatus |
| CN1583213A (en) * | 2004-06-11 | 2005-02-23 | 西安瑞捷生物医疗技术有限公司 | Apparatus for preparing thrombocyte enriched blood plasma and preparing method thereof |
| CN1261183C (en) * | 2004-06-11 | 2006-06-28 | 西安瑞捷生物医疗技术有限公司 | Apparatus for preparing thrombocyte enriched blood plasma and preparing method thereof |
| CN103974777A (en) * | 2011-11-01 | 2014-08-06 | 朴铉贞 | Container for centrifugal separation providing rapid centrifugal separation |
| CN104694382A (en) * | 2015-03-09 | 2015-06-10 | 余家昌 | Rapid nucleic acid extraction device and method |
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| CN106076442A (en) | 2016-11-09 |
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Effective date of registration: 20210305 Address after: 530000 no.66-303, Gaoling Road, Nanning, Guangxi Zhuang Autonomous Region Patentee after: NANNING TECBOD BIOTECHNOLOGY Co.,Ltd. Address before: 5 / F, No.8 workshop, 2879 Dongfu Avenue, Haicang District, Xiamen City, Fujian Province, 361027 Patentee before: TECBOD (XIAMEN) BIOTECHNOLOGY Co.,Ltd. |