CN105969686A - Bacterial-wilt-resistant Streptomyces rochei strain and bio-organic fertilizer thereof - Google Patents
Bacterial-wilt-resistant Streptomyces rochei strain and bio-organic fertilizer thereof Download PDFInfo
- Publication number
- CN105969686A CN105969686A CN201610329907.1A CN201610329907A CN105969686A CN 105969686 A CN105969686 A CN 105969686A CN 201610329907 A CN201610329907 A CN 201610329907A CN 105969686 A CN105969686 A CN 105969686A
- Authority
- CN
- China
- Prior art keywords
- strain
- bacterial
- wilt
- bio
- fertilizer
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/465—Streptomyces
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
- C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F3/00—Fertilisers from human or animal excrements, e.g. manure
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Fertilizers (AREA)
Abstract
The invention discloses a bacterial-wilt-resistant antagonistic bacterium strain. The strain is named Streptomyces rochei L-9, and was collected by China General Microbiological Culture Collection Center on September 25th, 2013; and the collection number is CGMCC No.8264. The strain L-9 has high bacterial wilt resistance, and the antagonistic ring radius reaches 15mm. The invention provides a new microbial resource for biological control on bacterial wilt, and overcomes the defects in the prior art.
Description
Technical field
The invention belongs to biological technical field, particularly to the Antagonistic Fungi of a kind of resistance to bacterial wilt, be specifically related to a kind of Lou thorough
Family name streptomycete (Streptomyces rochei) L-9 bacterial strain, relates to the application in terms of resistance to bacterial wilt of this bacterial strain simultaneously.
Background technology
Tobacco bacterial wilt is one of Major Diseases of tropical and subtropical region Nicotiana tabacum L., at present in Yangtze River in China basin and
On the south cigarette district generally occur, wherein with Guangdong, Fujian, Taiwan, Hunan, Jiangxi, east Guangxi, Southern Anhui Province, Sichuan and Guizhou
Locally cigarette district causes harm seriously, usually outbreak of epidemic of indivedual times, causes crushing loss.In recent years, the morbidity of this disease and be
Evil scope has the trend of north cigarette district extension, and in Shandong, Henan, Shaanxi and Liaoning etc. save has generation, some areas to cause harm
Heavier.The loss that this disease causes at present has occupied the 4th of all kinds of disease.The conventional strategy preventing and treating bacterial wilt include chemical pesticide,
Soil improvement, disease-resistant variety, grafting and crop rotation etc., but effect is the most unstable.Additionally, excess uses the environment that chemical pesticide brings
Pollute and food-safety problem, cause people's extensive concern and worry.Therefore, how development environment friendly prophylactic measures,
Realize reducing pesticide dosage, suppression disease develops, recovery soil health is the problem needing solution at present badly.
Summary of the invention
The technical problem to be solved is to provide Lou Che Shi streptomycete bacterial strain and the biology thereof of a kind of resistance to bacterial wilt
Fertilizer, for the microbial resources that the Biological control offer of bacterial wilt is new.
The Antagonistic Fungi of resistance to bacterial wilt of the present invention, this Strain Designation is Lou Che Shi streptomycete (Streptomyces
Rochei) L-9, was deposited in China Committee for Culture Collection of Microorganisms's common micro-organisms center on 09 25th, 2013
(Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3), deposit number is CGMCC No.8264.
The bacterial strain L-9 of the present invention has the property that
1, L-9 is on Gause I solid medium, and mycelia is flourishing, and milky bit brownish, center is black, and gas silk is rich
Cyclopentadienyl, velvet-like, Lycoperdon polymorphum Vitt.Base silk is colourless, and after switching, slightly blush is adjusted sometimes.
2, L-9 shows the performance of stronger antagonism Ralstonia solanacearum, and antagonism circle radius reaches 15mm.
3, bacterial genomes DNA small volume of reagent box (AxyPrep, the U.S.) high salt precipitation method is used to extract DNA.PCR expands
Universal primer is 27F/1541R.I.e. forward primer: 5 '-GAGAGTTTGATCCTGGCTCAG-3 '；Reverse primer: 5 '-
AAGGAGGTGATCCA GCCGCA-3′.16S rRNA gene order sequencing result shows, the 16S rRNA gene order of L-9 is
1488bp, the sequence Genbank in NCBI website online sequence alignment result shows, L-9 Yu Streptomyces rochei's
Homology, more than 99%, is accredited as Lou Che Shi streptomycete according to morphological characteristic and 16S rDNA.
The bacterial strain of the present invention is accessed the rapeseed cake fertilizer of rapeseed cake enzymolysis with cow dung compost with weight with the inoculation weight of 5%
Ratio fermentation culture in the fertilizer that 1:1 mixes, fermentation culture conditions is: temperature 30～45 DEG C, water content 30～40%, time
Between 4～5 days, rear water content of having fermented reaches 34.9%, is biological organic fertilizer.Bacterial wilt is had substantially by this biological organic fertilizer
Prevention effect.
Accompanying drawing explanation
Accompanying drawing 1 is the flat board antagonistic effect of Antagonistic Fungi L-9；
Accompanying drawing 2 is that the system that 16S rRNA gene order based on isolated strains and the close bacterial strain of its sibship builds is sent out
Educate tree (adjacent method)；
Accompanying drawing 3 is the quantity of Ralstonia solanacearum in potting media；
Accompanying drawing 4 is the quantity of Antagonistic Fungi L-9 in potting media.
Detailed description of the invention
The separation of embodiment 1 Antagonistic Fungi of the present invention L-9 and qualification
The separation of 1.1 Antagonistic Fungi L-9 and screening
Gather, from many places, Guizhou Province tobacco bacterial wilt morbidity field, the cigarette strain and Rhizosphere Soil, cryopreservation do not fallen ill, be used for
Separate Antagonistic Fungi.Process to cigarette strain sample: take rhizome tissue, first with 2% sodium hypochlorite surface sterilization 2min, then use
Deionization washing rinse 3～5 times after sterilizing.To pedotheque or or process after cigarette strain sample, according to dilution spread flat board
The soil liquid or Plant samples solution are diluted to 10 by method-1～10-6Stand-by.
The soil liquid or Plant samples solution are coated in Gause I culture medium uniformly, at 30 DEG C of dark conditions
Under, after cultivating 24h, after bacterium colony grows, Stalk Rot Ralstonia solanacearum bacteria suspension is uniformly sprayed on flat
On plate, carry out cultivating and observe.Separating from soil and cigarette strain, screen 219 strain antagonistic strains, its antagonism circle of comprehensive assessment is big
The indexs of correlation such as growing state under little, the speed of growth, lean nutrition, whether salt tolerant, finally choose a strain Antagonistic Fungi L-9.Often
Rule qualification result shows, L-9 is on Gause I solid medium, and mycelia is flourishing, milky bit brownish, and center is black,
Gas silk is luxuriant, velvet-like, Lycoperdon polymorphum Vitt.Base silk is colourless, and after switching, slightly blush is adjusted sometimes.L-9 shows stronger antagonism Ralstonia solanacearum
Performance, antagonism circle radius reaches 15mm.
The 16S rRNA gene identification of 1.2 Antagonistic Fungis and gene sequencing
Bacterial genomes DNA small volume of reagent box (AxyPrep, the U.S.) high salt precipitation method is used to extract DNA.PCR amplification is logical
It is 27F/1541R with primer.I.e. forward primer: 5 '-GAGAGTTTGATCCTGGCTCAG-3 '；Reverse primer: 5 '-
AAGGAGGTGATCCA GCCGCA-3′。
16S rRNA gene order sequencing result shows, the 16S rRNA gene order of L-9 is 1488bp, and sequence is at NCBI
The Genbank online sequence alignment result of website shows, the homology of L-9 Yu Streptomyces rochei more than 99%,
It is accredited as Lou Che Shi streptomycete (referring to Fig. 2) according to morphological characteristic and 16S rDNA.
1.3 bacterial strain L-9 production methods
1.3.1 prepared by bacterial strain primary seed solution
L-9 is in Gause I fluid medium, and in 30 DEG C of shaking tables, 170rpm cultivates 48h, makes primary seed solution.
1.3.2 function strain L-9 bacterial strain first order seed amplification culture
Test through repeatedly first order seed lab scale amplification culture, excellent to L-9 bacterial strain first order seed amplification culture technical parameter
Change, preliminarily form following scale up test technical parameter.
Culture medium prescription: PDA improved culture medium 1；
Fermentation pH: before disappearing 7.2, after disappearing 7.0；
Inoculation weight: 1%；
Cultivation temperature: 37 degree；
First order seed rotating speed: 160～170 revs/min；
Incubation time: 16～18h；
Bacteria containing amount: 2～3 × 108cfu/mL。
1.3.3 function strain L-9 bacterial strain secondary seed amplification culture
Test through repeatedly secondary seed lab scale amplification culture, L-9 bacterial strain secondary seed amplification culture technical parameter optimized,
Preliminarily form following scale up test technical parameter.
Culture medium prescription: No. 2+0.1% defoamer of PDA improved culture medium；
Cultivate pH: before disappearing 7.2, after disappearing 7.0；
Inoculation weight: 5%；
Cultivation temperature: 35 DEG C；
Two grades of cultivation rotating speeds: 45～50Hz；
Ventilation: 35～40m3/h；
Pressure: 0.045～0.05MPa；
Dissolved oxygen: 80～85%；
Incubation time: 17～18h；
Bacteria containing amount: 1～2 × 109cfu/mL。
1.3.4 function strain L-9 bacterial strain 500L amplification culture
Test through repeatedly amplification culture lab scale, L-9 bacterial strain amplification culture technical parameter is optimized, preliminarily forms following pilot scale
Technological production norm.
Culture medium prescription: No. 3+0.15% defoamer of PDA improved culture medium；
Fermentation pH: before disappearing 7.2, after disappearing 7.0；
Inoculation weight: 10%；
Cultivation temperature: 32 DEG C；
Amplification culture rotating speed: 75～80Hz；
Ventilation: 60～80m3/h；
Pressure: 0.045～0.05MPa；
Dissolved oxygen: early stage 60～75%, mid-term 90～100%, the later stage 75～100%；
Incubation time: 24～27h；
Bacteria containing amount: 4～5 × 109Cfu/mL (spore rate >=90%).
The preparation of embodiment 2 biological organic fertilizer of the present invention
Cultivating Antagonistic Fungi L-9 in Gause I fluid medium, in 30 DEG C of shaking tables, 170rpm cultivates 48h.Organic
The fertile rapeseed cake fertilizer for rapeseed cake enzymolysis mixes with weight ratio 1:1 with cow dung compost, after measured containing organic matter 33.8%, ammonia
Base acid 4.3%, N4.20%, P2O52.26%, K2O1.08%.Antagonistic Fungi L-9 culture fluid is accessed with the inoculation weight of 5%
Stating in fertilizer, regulation temperature is 30～45 DEG C, and water content is 30～40%, ferments 4～5 days, and period constantly turns over throwing.Ferment
After one-tenth, water content reaches 34.9%, is biological organic fertilizer (hereinafter referred to as " BIO ").
Embodiment 3 BIO effect of resistance to bacterial wilt in seedling growth test
Experimental cultivar: the big gold dollar of K326 Flos Carthami.
Earthworm excrement seedling medium: preparation method is shown in patent ZL200710202167.6.
3.1 EXPERIMENTAL DESIGN
3.1.1 the BIO impact on Nicotiana tabacum L. germination percentage
Every 100 K326 seeds of flat board or Flos Carthami big gold dollar seed, substrate is added different proportion by conventional seedbed system substrate
Earthworm excrement seedling medium and BIO form, and process and are shown in Table 1.1, and about paving 1cm is thick, and each process repeats for 5 times, in illumination box 25 DEG C,
75% moisture content is cultivated, results of regular determination bud ratio.
Tobacco seed is sprouted and is affected EXPERIMENTAL DESIGN by table 1.1 BIO
3.1.2 the impact that tobacco in seedling stage is grown by BIO
The result affected Nicotiana tabacum L. germination percentage according to BIO, using to sprout Nicotiana tabacum L. does not has ratio (the i.e. earthworm excrement of inhibitory action
Seedling medium 68%, BIO 2%) apply Nicotiana tabacum L. conventional seedbed system substrate.Being respectively adopted two kinds of method for culturing seedlings, a kind of is conventional drift
Floating nursery, another kind of in view of the growth and breeding problem of Antagonistic Fungi in BIO, use black plastic seedlings nursing plate educating relative to floating
Nursery under conditions of Seedling is more arid, is i.e. directly placed in black plastic seedlings nursing plate in nursery groove, and about 1cm is only saved in bottom
Water.Test processes and is shown in Table 1.2.
Table 1.2 BIO nursery processes
3.1.3 the BIO impact on tobacco seedling growth in pot experiment
Process by table 1.3 transplants in new seedling medium by tobacco seedlings, every basin 10Kg seedling medium, often processes 12 weights
Multiple.In seedling medium, add a certain proportion of Ralstonia solanacearum bacteria suspension, BIO or rapeseed cake fertilizer fermented, make Rs
Concentration reaches 107cfu g-1Substrate, and BIO and rapeseed cake fertilizer add with the ratio of every basin 50g respectively.Respectively with nursery stage pair
Compare substrate-pathogen substrate two according to substrate-transplant health substrate and nursery stage to process as comparison.Transplant and gathered the same day
Different substrates sample, gathered a rhizosphere substrate every 10 days and adds up sickness rate, measures base with fluorescence quantifying PCR method later
Pathogen and the quantity of Antagonistic Fungi in quality sample.
The potted plant process of table 1.3 BIO nursery
Note: be process during nursery before "-".Being the process after transplanting after "-", wherein, CK represents that blank processes, n
Representing that substrate seedling processes, p represents potted plant process；+ Rs represents addition Ralstonia solanacearum in soil；OF refers to not add antagonism
The fertilizer of bacterium L-9.
3.2 results:
3.2.1 the BIO impact on Nicotiana tabacum L. germination percentage
Bud ratio testing result is shown in Table 2.1.Compared with the control, when BIO concentration is within 2%, the germination percentage of Nicotiana tabacum L. with
Comparison is compared, without significant difference；When BIO concentration is higher than 2%, germination percentage is substantially reduced.
Table 2.1 BIO impact on Tobacco Seed Germination rate
| Process | CK | T1 | T2 | T3 | T4 | T5 |
| Germination percentage (%) | 97.3 | 93.3 | 95.6 | 68.3 | 53.7 | 53.3 |
3.2.2 BIO nursery passes the impact of bacterial wilt sickness rate to Nicotiana tabacum L. germination percentage and soil
Tobacco seedlings is transplanted the sickness rate of latter 30 days statistics pot experiments and is referred to table 2.2.From table 2.2 it can be seen that K326 green grass or young crops is withered
Sick sickness rate is the most relatively low, the most only investigates in the substrate of the big gold dollar of Flos Carthami pathogen and Antagonistic Fungi in the change of different times
Change.
Table 2.2 different disposal passes the impact of bacterial wilt sickness rate to Nicotiana tabacum L. soil
30 days sampling results from Fig. 3 it can be seen that BIO2-BIOp+Rs HD (i.e. drought cultivates Seedling, nursery and potted plant disease
Soil all applies BIO) process relative to other, pathogen quantity can be preferably minimized.This result is likely due to nursery
Period Antagonistic Fungi is grown at tobacco seedlings rhizosphere the most surely, and therefore, in the presence of having pathogen in pot experiment substrate, Antagonistic Fungi is in the same old way
Can play a role, the quantity of pathogen can be reduced from the beginning, play the effect of protection cigarette strain.
From fig. 4, it can be seen that Antagonistic Fungi also can present a kind of trend first dropping and rising afterwards in substrate.And relative to traditional
Floating seedlings, the Antagonistic Fungi of drought training is more easy to existence, and therefore after tobacco seedlings is transplanted, the quantity of its Antagonistic Fungi is also most.During nursery
Just adding Antagonistic Fungi, add the process of Antagonistic Fungi than after tobacco seedlings is transplanted, Antagonistic Fungi can enter build phase quickly, by
This ensure that Antagonistic Fungi quantity in substrate, so provides for sound assurance for antagonism pathogen.
Claims (6)
1. an Antagonistic Fungi for resistance to bacterial wilt, this Strain Designation is Lou Che Shi streptomycete (Streptomyces rochei) L-9,
Being deposited in China Committee for Culture Collection of Microorganisms's common micro-organisms center on 09 25th, 2013, deposit number is
CGMCC No.8264。
The bacterial strain the most according to claim 1 application in resistance to bacterial wilt.
3. the preparation method of a biological organic fertilizer, it is characterised in that: by the bacterial strain described in claim 1 with 5% inoculation weight
Amount accesses fermentation culture in fertilizer.
Preparation method the most according to claim 3, it is characterised in that: described fertilizer is the rapeseed cake fertilizer of rapeseed cake enzymolysis
Mix with weight ratio 1:1 with cow dung compost.
Preparation method the most according to claim 3, it is characterised in that described fermentation culture conditions is: temperature 30～45 DEG C,
Water content 30～40%, time 4～5 days, rear water content of having fermented reaches 34.9%.
6. the biological organic fertilizer prepared according to method described in claim 3～5 any claim application in resistance to bacterial wilt.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610329907.1A CN105969686A (en) | 2016-05-18 | 2016-05-18 | Bacterial-wilt-resistant Streptomyces rochei strain and bio-organic fertilizer thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610329907.1A CN105969686A (en) | 2016-05-18 | 2016-05-18 | Bacterial-wilt-resistant Streptomyces rochei strain and bio-organic fertilizer thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105969686A true CN105969686A (en) | 2016-09-28 |
Family
ID=56956572
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610329907.1A Withdrawn CN105969686A (en) | 2016-05-18 | 2016-05-18 | Bacterial-wilt-resistant Streptomyces rochei strain and bio-organic fertilizer thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105969686A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108300681A (en) * | 2018-04-20 | 2018-07-20 | 海南大学 | One plant of Lou Che Shi streptomycete and its application |
| CN109874808A (en) * | 2019-01-25 | 2019-06-14 | 青岛农业大学 | The application of arbuscular mycorrhizal fungi and/or plant symbiosis actinomyces in the biological prevention and control agent that preparation is used for capsicum and eggplant |
| CN114456973A (en) * | 2022-01-11 | 2022-05-10 | 湖北省生物农药工程研究中心 | Streptomyces rochei strain in tobacco and application thereof in prevention and control of tobacco diseases |
| CN118497064A (en) * | 2024-06-06 | 2024-08-16 | 江苏沿海生态科技发展有限公司 | Saline-alkali soil salt-tolerant actinomycetes and application thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105309082A (en) * | 2015-10-21 | 2016-02-10 | 贵州省烟草科学研究院 | Comprehensive preventing and controlling method for tobacco bacterial wilt |
-
2016
- 2016-05-18 CN CN201610329907.1A patent/CN105969686A/en not_active Withdrawn
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105309082A (en) * | 2015-10-21 | 2016-02-10 | 贵州省烟草科学研究院 | Comprehensive preventing and controlling method for tobacco bacterial wilt |
Non-Patent Citations (2)
| Title |
|---|
| YANXIA LIU等: "Tobacco bacterial wilt can be biologically controlled by the application of antagonistic strains in combination with organic fertilizer", 《BIOL. FERTIL. SOILS.》 * |
| 刘艳霞: "土传烟草青枯病的生物防控及其机理研究", 《中国博士学位论文全文数据库 农业科技辑》 * |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108300681A (en) * | 2018-04-20 | 2018-07-20 | 海南大学 | One plant of Lou Che Shi streptomycete and its application |
| CN108300681B (en) * | 2018-04-20 | 2021-07-23 | 海南大学 | Streptomyces rochei and application thereof |
| CN109874808A (en) * | 2019-01-25 | 2019-06-14 | 青岛农业大学 | The application of arbuscular mycorrhizal fungi and/or plant symbiosis actinomyces in the biological prevention and control agent that preparation is used for capsicum and eggplant |
| CN109874808B (en) * | 2019-01-25 | 2021-02-02 | 青岛农业大学 | Application of arbuscular mycorrhizal fungi and/or plant symbiotic actinomycetes in preparation of biocontrol preparation for hot pepper and solanaceae vegetables |
| CN114456973A (en) * | 2022-01-11 | 2022-05-10 | 湖北省生物农药工程研究中心 | Streptomyces rochei strain in tobacco and application thereof in prevention and control of tobacco diseases |
| CN114456973B (en) * | 2022-01-11 | 2022-08-05 | 湖北省生物农药工程研究中心 | Streptomyces rochei in tobacco and application thereof in prevention and control of tobacco diseases |
| CN118497064A (en) * | 2024-06-06 | 2024-08-16 | 江苏沿海生态科技发展有限公司 | Saline-alkali soil salt-tolerant actinomycetes and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| WO2021185021A1 (en) | Microbial agent yf beneficial to corn growth in saline-alkali soil and application thereof | |
| CN112266881B (en) | Bacillus amyloliquefaciens and application thereof in preventing and treating apple continuous cropping obstacle | |
| CN104726383A (en) | Bacillus amyloliquefaciens JK6 and biological fertilizer and application | |
| CN106222118B (en) | One streptomycete category actinomyces and application thereof | |
| CN109097303B (en) | Paenibacillus polymyxa, spore suspension of paenibacillus polymyxa, microbial seedling culture substrate and preparation method of microbial seedling culture substrate | |
| CN110669691B (en) | Bacillus megaterium for preventing and treating plant nematode diseases and application thereof | |
| CN103642734B (en) | Microbacterium maritypicum and application thereof in preventing sugar beet disease-causing organisms | |
| CN111763643A (en) | Compound flora for preventing and treating peanut root rot and application thereof | |
| CN105969686A (en) | Bacterial-wilt-resistant Streptomyces rochei strain and bio-organic fertilizer thereof | |
| CN111073827B (en) | Bacillus beilesensis JTB8-2 and application thereof in biological control of orobanche meloidogyne | |
| CN106701631A (en) | Strain of streptomyces roseoflavus and application thereof | |
| CN106399148A (en) | Kosakonia radicincitans and application thereof | |
| CN103173386B (en) | Bio-control strain G1 for preventing and controlling pepper phytophthora blights and applications thereof | |
| CN108913625B (en) | Salt-tolerant streptomycete, microbial inoculum thereof and application of microbial inoculum thereof in promoting plant growth | |
| CN104789494B (en) | The method for improving turf salt-resistance using garbage compost microbial bacterial agent is strengthened | |
| CN115960766A (en) | Microorganism for preventing and treating bacterial wilt and application thereof | |
| CN103981101A (en) | DSE (Dark Septate Endophyte) strain and application of DSE strain in production of sugarcane | |
| CN106148250B (en) | One plant of Costa Rica streptomyces actinomyces and application thereof | |
| CN104108964A (en) | Preparation method and application of total nutrient microorganism biocontrol organic fertilizer | |
| CN110205249B (en) | Method for promoting plant growth and alternaria alternate fungus used by same | |
| CN107325980A (en) | A kind of radiation hardness series bacillus KH9 and its application in biological antitranspirant | |
| CN114934000B (en) | Salt-tolerant bacillus amyloliquefaciens and screening and application thereof | |
| CN113897316B (en) | Bacillus licheniformis BLc06, functional melon and fruit seedling biological matrix prepared from same and application | |
| CN116676234A (en) | Salt-tolerant bacillus JK-25, microbial inoculum, preparation method and application thereof | |
| CN105462882A (en) | Pseudomonas aeruginosa for preventing and treating vertieillium wilt in crops and application of pseudomonas aeruginosa |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WW01 | Invention patent application withdrawn after publication | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20160928 |