CN105918355A - Special green muscardine fungus insecticide for preventing and treating Pleonomus canaliculatus, and preparation method thereof - Google Patents
Special green muscardine fungus insecticide for preventing and treating Pleonomus canaliculatus, and preparation method thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/36—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings
- A01N43/38—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings condensed with carbocyclic rings
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N57/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds
- A01N57/10—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-oxygen bonds or phosphorus-to-sulfur bonds
- A01N57/14—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-oxygen bonds or phosphorus-to-sulfur bonds containing aromatic radicals
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
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Abstract
The invention provides a special green muscardine fungus insecticide for preventing and treating Pleonomus canaliculatus, and a preparation method thereof. The insecticide comprises the following raw materials by mass, 14 to 23 parts of phoxim, 3 to 5.2 parts of Metarhizium anisopliae, 2.3 to 4 parts of balsam pear extract, 1.5 to 2.6 parts of powder of Cyrtomium fortune, 0.6 to 1.3 parts of indolebutyric acid, 1.5 to 2.5 parts of a dispersing agent, 1.9 to 3.3 parts of a wetting agent, 9 to 15 parts of disodium EDTA and 15 to 25 parts of water. The invention has the beneficial effects that the insecticide prepared in the invention has good prevention and treatment effect on insects in a field planted with wheat, and prevention effect on common insects in the field such as Pleonomus canaliculatus, Chinese mole crickets, grubs and cutworms reaches 97.5-99.8%, 94.9-98.4% and 93.2-96.7%, respectively.
Description
Technical field
The present invention relates to a kind of Metarhizium anisopliae insecticide being exclusively used in pleonomus canaliculatus preventing and treating and preparation method thereof, belong to
Technical field of pesticide.
Background technology
Metarhizium anisopliae (Metarhizium anisopliae), belong to Ascomycota, Hypocreales, Clavicipitaceae,
Metarhizium, is the disinsection fungal of a kind of broad spectrum activity.In nature, it is substantially carried out asexual propagation, insect body wall carries out actively
Invade and infect different insects, insecticide haemocoele can carry out yeast shape breeding, produce a large amount of hyphal body, destruction can be produced simultaneously
The Pesticidal toxins such as element (destuxin, Non-ribosomal peptide toxoid), suppress Insect immunity, acceleration of killing.At insect host not
In the presence of, Metarhizium anisopliae also can form rhizosphere symbiosis with plant root and harbor for a long time.
Metarhizium anisopliae includes adhesion, spore germination, generation appresorium, penetrates polypide, internal host's infection processs
Grow and lethal.This process is the comprehensive knot of appresorium, skin degradation enzyme and the Physiology and biochemistry effect destroying the materials such as rhzomorph
Really.Owing to its host range is wide, pathogenicity strong, harmless to people, animal, crops, easily carry out large-scale production, and can produce
Raw Sustainable Control effects etc., have wide biological control of insect pests application prospect.
Crops are all had a certain degree of Side effect by existing chemical insecticide, can cause soil in various degree
The environmental pollutions such as ground, water source, chemical insecticide improper use can cause crops to produce poisoning, causes crop growth abnormal,
Suppress its photosynthesis, affect crop yield, and second stubble crop be will also result in certain murder by poisoning.
Existing chemical insecticide, is divided into liquid and solid preparation two types, liquid preparation dispersible oil-suspending agent,
Cream, aqueous emulsion, microemulsion；Solid preparation has wettable powder, soluble powder, water dispersible granules.
The defect that existing chemical insecticide exists is as follows:
(1) existing insecticide, just for the insect pest in planting site, not notable or the most anti-to the diseases control in planting site
Effect, and existing insecticide is the most obvious to certain insect insecticidal effect；
(2), when existing insecticide is applied to Semen Tritici aestivi pest control, Semen Tritici aestivi is had certain side effect, poor stability, it is grown
There is certain inhibitory action, and then reduce crop yield.
(3) after existing insecticide has been used, easily causing insecticide toxin residual, the quality of Semen Tritici aestivi produces impact,
Affect the edible safety of Semen Tritici aestivi.
Summary of the invention
The present invention solves the deficiency that prior art exists, it is provided that a kind of green deadlock of chafer being exclusively used in pleonomus canaliculatus preventing and treating
Bacteria pesticide and preparation method thereof, to realize following goal of the invention:
(1) insecticide prepared by the present invention is good to the prevention effect of the insect in wheat planting ground, to common in wheat planting ground
Insect: pleonomus canaliculatus, Gryllotalpa, Holotrichia diomphalia Bates, the preventive effect of cutworm significantly improve；
(2) while insecticide prepared by the present invention insect in carrying out wheat planting ground, the disease control to wheat plant
There is effect equally；
(3), during insecticide prepared by the present invention is applied to salt-soda soil Semen Tritici aestivi during pest control, Semen Tritici aestivi is had no side effect, safety
High.Insect host not in the presence of, Metarhizium anisopliae also can with plant root formed rhizosphere symbiosis and carry out long-term place
Deposit, promote plant growing.
For solving above technical problem, the technical solution used in the present invention is as follows:
A kind of Metarhizium anisopliae insecticide being exclusively used in pleonomus canaliculatus preventing and treating, described insecticide is green including phoxim, chafer
Stiff bacterium, Fructus Momordicae charantiae extract, Rhizoma Osmundae powder, indolebutyric acid.
The following is and the further of technique scheme is improved:
Described insecticide, the mass parts content of each raw material components is: phoxim 14-23 part, Metarhizium anisopliae 3*5.2 part, hardship
Melon extract 2.3-4 part, Rhizoma Osmundae powder 1.5-2.6 part, indolebutyric acid 0.6-1.3 part, dispersant 1.5-2.5 part, wetting agent 1.9-
3.3 parts, EDETATE DISODIUM 9-15 part, water 15-25 part.
Described wetting agent is sodium butylnaphthalenesulfonate salt.
Described dispersant is naphthalenesulfonate formaldehyde condensation compound.
Described Fructus Momordicae charantiae extract contains Charantin and Fructus Momordicae charantiae fat eliminating element, and Charantin content is 35%, and Fructus Momordicae charantiae fat eliminating cellulose content is
2%。
Described Rhizoma Osmundae powder contains thick filicin and tannic acid, and thick filicin content is 49.17%, and tanin content is 14.5%.
A kind of Metarhizium anisopliae insecticide being exclusively used in pleonomus canaliculatus preventing and treating and preparation method thereof, described preparation method bag
Including the preparation of Fructus Momordicae charantiae extract, the preparation of described Fructus Momordicae charantiae extract includes reflux, extract, step, absorb-elute step, extraction step
Suddenly, concentration step.
Described reflux, extract, step, takes Fructus Momordicae charantiae and removes seed, pulverizes, reflux, extract, after soaking with 14 times amount 80% ethanol waters
For several times, extract 5h every time, filter, obtain ethanol extract and filtering residue, merge ethanol extract, stand 48h, filter off precipitation, reclaim
Solvent obtains crude extract.
Described absorb-elute step, crude extract reflux, extract, step prepared is water-soluble, upper macroporous adsorptive resins, washing
After desaccharide, with 95% ethanol water eluting, collecting ethanol elution, recycling design obtains extract.
Described insecticide consumption is 20-45g/ mu.
Use above technical scheme, the invention have the benefit that
(1) insecticide prepared by the present invention is good to the prevention effect of the insect in wheat planting ground, to common in wheat planting ground
Insect: pleonomus canaliculatus, Gryllotalpa, Holotrichia diomphalia Bates, the preventive effect of cutworm respectively reach 97.5-99.8%, 94.9-98.4%, 93.2-
96.7%。
(2) while insecticide prepared by the present invention insect in carrying out wheat planting ground, the disease to wheat plant
Preventing and treating has effect equally.Disease to common in wheat planting ground: take-all, Semen Tritici aestivi Cyst nematode, banded sclerotial blight, disease
The preventive effect of viral disease respectively reaches 85.9-89.6%, 86.5-92.3%, 81.8-85.4%, 78.3-80.9%.
(3) insecticide that prepared by the present invention is without any side effects to wheat plant, and safety is high, also obtains beyond thought
Effect, the effect of being significantly improved of the yield of wheat plant, per mu yield reaches 1090-1250 jin；Protein and dietary fiber in Semen Tritici aestivi
Content also significantly improves, and protein content is 10.5-11.9g/100g, and crude fiber content is 8.3-9.1g/100g.
(4) insect host not in the presence of, Metarhizium anisopliae also can with plant root formed rhizosphere symbiosis and enter
Row harbors for a long time, promotes plant growing.
Detailed description of the invention
Hereinafter the preferred embodiments of the present invention are illustrated, it will be appreciated that preferred embodiment described herein is only used
In the description and interpretation present invention, it is not intended to limit the present invention.
Embodiment 1 one kinds is exclusively used in the Metarhizium anisopliae insecticide of pleonomus canaliculatus preventing and treating
Described insecticide, the mass ratio of each raw material components is:
Phoxim 14 parts, Metarhizium anisopliae 3 parts, Fructus Momordicae charantiae extract 2.3 parts, 1.5 parts of Rhizoma Osmundae powder, indolebutyric acid 0.6 part, dispersion
Agent 1.5 parts, wetting agent 1.9 parts, EDETATE DISODIUM 9 parts, 15 parts of water.
Described phoxim, the weight/mass percentage composition of effective ingredient is 25%；
Described Metarhizium anisopliae, the mass content of effective ingredient is 20,000,000,000 spores/g；
Described Fructus Momordicae charantiae extract contains Charantin and Fructus Momordicae charantiae fat eliminating element, and Charantin content is 35%, and Fructus Momordicae charantiae fat eliminating cellulose content is 2%；
Described Rhizoma Osmundae powder contains thick filicin and tannic acid, and thick filicin content is 49.17%, and tanin content is 14.5%；
Described indolebutyric acid, the weight/mass percentage composition of effective ingredient is 98%；
Described wetting agent is sodium butylnaphthalenesulfonate salt,；
Described dispersant is naphthalenesulfonate formaldehyde condensation compound；
Described EDETATE DISODIUM, the weight/mass percentage composition of effective ingredient is 99%.
Embodiment 2 one kinds is exclusively used in the Metarhizium anisopliae insecticide of pleonomus canaliculatus preventing and treating
Described insecticide, the mass ratio of each raw material components is:
Phoxim 18 parts, Metarhizium anisopliae 4 parts, Fructus Momordicae charantiae extract 3 parts, 2 parts of Rhizoma Osmundae powder, indolebutyric acid 1 part, dispersant 2 parts,
Wetting agent 2.5 parts, EDETATE DISODIUM 12 parts, 20 parts of water.
Described phoxim, the weight/mass percentage composition of effective ingredient is 25%；
Described Metarhizium anisopliae, the mass content of effective ingredient is 20,000,000,000 spores/g；
Described Fructus Momordicae charantiae extract contains Charantin and Fructus Momordicae charantiae fat eliminating element, and Charantin content is 35%, and Fructus Momordicae charantiae fat eliminating cellulose content is 2%；
Described Rhizoma Osmundae powder contains thick filicin and tannic acid, and thick filicin content is 49.17%, and tanin content is 14.5%；
Described indolebutyric acid, the weight/mass percentage composition of effective ingredient is 98%；
Described wetting agent is sodium butylnaphthalenesulfonate salt,；
Described dispersant is naphthalenesulfonate formaldehyde condensation compound；
Described EDETATE DISODIUM, the weight/mass percentage composition of effective ingredient is 99%.
Embodiment 3 one kinds is exclusively used in the Metarhizium anisopliae insecticide of pleonomus canaliculatus preventing and treating
Described insecticide, the mass ratio of each raw material components is:
Phoxim 23 parts, Metarhizium anisopliae 5.2 parts, Fructus Momordicae charantiae extract 4 parts, 2.6 parts of Rhizoma Osmundae powder, indolebutyric acid 1.3 parts, dispersion
Agent 2.5 parts, wetting agent 3.3 parts, EDETATE DISODIUM 15 parts, 25 parts of water.
Described phoxim, the weight/mass percentage composition of effective ingredient is 25%；
Described Metarhizium anisopliae, the mass content of effective ingredient is 20,000,000,000 spores/g；
Described Fructus Momordicae charantiae extract contains Charantin and Fructus Momordicae charantiae fat eliminating element, and Charantin content is 35%, and Fructus Momordicae charantiae fat eliminating cellulose content is 2%；
Described Rhizoma Osmundae powder contains thick filicin and tannic acid, and thick filicin content is 49.17%, and tanin content is 14.5%；
Described indolebutyric acid, the weight/mass percentage composition of effective ingredient is 98%；
Described wetting agent is sodium butylnaphthalenesulfonate salt,；
Described dispersant is naphthalenesulfonate formaldehyde condensation compound；
Described EDETATE DISODIUM, the weight/mass percentage composition of effective ingredient is 99%.
Metarhizium anisopliae used by above example 1 to embodiment 3 is buied by existing product on market.
Embodiment 4 one kinds is exclusively used in Metarhizium anisopliae insecticide of pleonomus canaliculatus preventing and treating and preparation method thereof
Described vegetable insecticide, dosage form is water dispersible granules, and preparation method includes:
(1) preparation of (1) Fructus Momordicae charantiae extract:
A, reflux, extract,
Taking Fructus Momordicae charantiae and remove seed, pulverize, after soaking with 14 times amount 80% ethanol waters, reflux, extract, is for several times, extracts 5h every time, filters,
Obtaining ethanol extract and filtering residue, merge ethanol extract, stand 48h, filter off precipitation, recycling design obtains crude extract；
B, absorption, eluting
By water-soluble for above-mentioned crude extract, upper macroporous adsorptive resins, after washing desaccharide, with 95% ethanol water eluting, collect ethanol
Eluent, recycling design obtains extract；
C, extraction
By water-soluble for said extracted thing, successively with 15 times amount dichloromethane, ethyl acetate, n-butanol extraction；
D, concentration
Being concentrated by remaining aqueous phase after above-mentioned extraction, being washed till concentrate with methanol can not be dissolved again, obtains final extract.
(2) weigh
According to the insecticidal formulation described in embodiment 1-3, weigh each composition.
(3) mixing
By phoxim, Metarhizium anisopliae, Fructus Momordicae charantiae extract, Rhizoma Osmundae powder, indolebutyric acid, dispersant, wetting agent and EDETATE SODIUM
Salt puts in blender and fully mixes；
(4) pulverize
Solid material after above-mentioned mixing is put in jet mill, is crushed to suitable fineness through jet mill and divides
Cloth；
(5) mediate
Putting in kneader by above-mentioned crushing rear material, uniformly add water in kneader kneading；
(6) pelletize, be dried
Above-mentioned kneaded wet stock is put into extruding pelletization in comminutor, the wet granular of extrusion is gone in fluid bed,
Being dried under the airflow function of proper temperature, in product, residual moisture content controls to be advisable in 0.5～1.0%, prepares insecticide.
Embodiment 5 one kinds is exclusively used in the Metarhizium anisopliae insecticide of pleonomus canaliculatus preventing and treating and applies in wheat planting ground
Four experimental plots, respectively experimental plot A, experimental plot B, experimental plot C and examination is hewed out in wheat planting ground
Testing community D, the length of each experimental plot is about 10-15m, and insecticide consumption is 20-45g/ mu.
Experimental plot A: by 800 times, product in embodiment 1 is watered dilution, uses the wheat plant in the A of experimental plot
Parasite killing liquid irrigating root, in Semen Tritici aestivi sowing time, seedling stage, turn green jointing-booting stage and heading the flowering and grouting phase respectively execute 2 medicines, dispenser
Time is separated by 7-10 days.
Experimental plot B: by 800 times, product in embodiment 2 is watered dilution, uses the wheat plant in the B of experimental plot
Parasite killing liquid irrigating root, in Semen Tritici aestivi sowing time, seedling stage, turn green jointing-booting stage and heading the flowering and grouting phase respectively execute 2 medicines, dispenser
Time is separated by 7-10 days.
Experimental plot C: by 800 times, product in embodiment 3 is watered dilution, uses the wheat plant in the C of experimental plot
Parasite killing liquid irrigating root, in Semen Tritici aestivi sowing time, seedling stage, turn green jointing-booting stage and heading the flowering and grouting phase respectively execute 2 medicines, dispenser
Time is separated by 7-10 days.
Experimental plot D: as controlled trial community, does not use this insecticide, replaces insecticide to experimental plot D with clear water
In the uniform pouring root of wheat plant.
Investigation and statistical method: investigation in 20 days after heading flowering and grouting phase dispenser, it is little that 100 strains are investigated in each experimental plot
Wheat, adds up killed strain number, checks prevention effect.
Result of the test:
(1) insecticide prepared by the present invention is good to the prevention effect of the insect in wheat planting ground, to common in wheat planting ground
Insect: pleonomus canaliculatus, Gryllotalpa, Holotrichia diomphalia Bates, the preventive effect of cutworm respectively reach 97.5-99.8%, 94.9-98.4%, 93.2-
96.7%, specific targets are shown in Table 1.
Insecticide prepared by table 1 present invention prevention effect to the insect in wheat planting ground.
(2) while insecticide prepared by the present invention insect in carrying out wheat planting ground, the disease to wheat plant
Preventing and treating has effect equally.
Disease to common in wheat planting ground: take-all, Semen Tritici aestivi Cyst nematode, banded sclerotial blight, virosis anti-
Effect respectively reaches 85.9-89.6%, 86.5-92.3%, 81.8-85.4%, 78.3-80.9%, and specific targets are shown in Table 2.
Insecticide prepared by table 2 present invention prevention effect to the disease in wheat planting ground.
(3) insecticide that prepared by the present invention is without any side effects to wheat plant, and safety is high, also obtains beyond thought
Effect, the effect of being significantly improved of the yield of wheat plant, per mu yield reaches 1090-1250 jin；Protein and dietary fiber in Semen Tritici aestivi
Content also significantly improves, and protein content is 10.5-11.9g/100g, and crude fiber content is 8.3-9.1g/100g, is specifically shown in Table
3。
Protein and dietary fiber content in the yield of table 3 Semen Tritici aestivi, Semen Tritici aestivi.
Comparison: do not use insecticide of the present invention, replaces insecticide to spray with clear water.
Finally it is noted that the foregoing is only the preferred embodiments of the present invention, it is not limited to the present invention,
Although being described in detail the present invention with reference to previous embodiment, for a person skilled in the art, it still may be used
So that the technical scheme described in foregoing embodiments to be modified, or wherein portion of techniques feature is carried out equivalent.
All within the spirit and principles in the present invention, any modification, equivalent substitution and improvement etc. made, should be included in the present invention's
Within protection domain.
Claims (10)
1. the Metarhizium anisopliae insecticide being exclusively used in pleonomus canaliculatus preventing and treating, it is characterised in that: described insecticide, including pungent
Sulfur phosphorus, Metarhizium anisopliae, Fructus Momordicae charantiae extract, Rhizoma Osmundae powder, indolebutyric acid.
A kind of Metarhizium anisopliae insecticide being exclusively used in pleonomus canaliculatus preventing and treating the most according to claim 1, its feature exists
In: described insecticide, the mass parts content of each raw material components is: phoxim 14-23 part, Metarhizium anisopliae 3*5.2 part, Fructus Momordicae charantiae
Extract 2.3-4 part, Rhizoma Osmundae powder 1.5-2.6 part, indolebutyric acid 0.6-1.3 part, dispersant 1.5-2.5 part, wetting agent 1.9-3.3
Part, EDETATE DISODIUM 9-15 part, water 15-25 part.
A kind of Metarhizium anisopliae insecticide being exclusively used in pleonomus canaliculatus preventing and treating the most according to claim 2, its feature exists
In: described wetting agent is sodium butylnaphthalenesulfonate salt.
A kind of Metarhizium anisopliae insecticide being exclusively used in pleonomus canaliculatus preventing and treating the most according to claim 2, its feature exists
In: described dispersant is naphthalenesulfonate formaldehyde condensation compound.
A kind of Metarhizium anisopliae insecticide being exclusively used in pleonomus canaliculatus preventing and treating the most according to claim 2, its feature exists
In: described Fructus Momordicae charantiae extract contains Charantin and Fructus Momordicae charantiae fat eliminating element, and Charantin content is 35%, and Fructus Momordicae charantiae fat eliminating cellulose content is 2%.
A kind of Metarhizium anisopliae insecticide being exclusively used in pleonomus canaliculatus preventing and treating the most according to claim 2, its feature exists
In: described Rhizoma Osmundae powder contains thick filicin and tannic acid, and thick filicin content is 49.17%, and tanin content is 14.5%.
7. Metarhizium anisopliae insecticide being exclusively used in pleonomus canaliculatus preventing and treating and preparation method thereof, it is characterised in that: described
Preparation method includes that the preparation of Fructus Momordicae charantiae extract, the preparation of described Fructus Momordicae charantiae extract include that reflux, extract, step, absorb-elute walk
Suddenly, extraction step, concentration step.
A kind of Metarhizium anisopliae insecticide being exclusively used in pleonomus canaliculatus preventing and treating the most according to claim 7 and preparation side thereof
Method, it is characterised in that: described reflux, extract, step, take Fructus Momordicae charantiae and remove seed, pulverize, return after soaking with 14 times amount 80% ethanol waters
Stream extracts for several times, extracts 5h every time, filters, and obtains ethanol extract and filtering residue, merges ethanol extract, stands 48h, and it is heavy to filter off
Forming sediment, recycling design obtains crude extract.
A kind of Metarhizium anisopliae insecticide being exclusively used in pleonomus canaliculatus preventing and treating the most according to claim 7 and preparation side thereof
Method, it is characterised in that: described absorb-elute step, crude extract reflux, extract, step prepared is water-soluble, upper macroporous adsorbent resin
Post, after washing desaccharide, with 95% ethanol water eluting, collects ethanol elution, and recycling design obtains extract.
10. one kind is exclusively used in the Metarhizium anisopliae insecticide of pleonomus canaliculatus preventing and treating and answering in the wheat planting of salt-soda soil thereof
With, it is characterised in that: described insecticide consumption is 20-45g/ mu.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106490066A (en) * | 2016-10-31 | 2017-03-15 | 郑州思辩科技有限公司 | A kind of microbial environment protecting insecticide and preparation method thereof |
| CN110169421A (en) * | 2019-05-20 | 2019-08-27 | 华中农业大学 | Application of the Metarhizium anisopliae in preparation prevention and treatment plant root-knot nematodes drug |
| CN110438016A (en) * | 2019-09-05 | 2019-11-12 | 江西省科学院微生物研究所 | A kind of Metarhizium anisopliae bacterial strain and its application |
| GB2577250A (en) * | 2018-09-14 | 2020-03-25 | Bionema Ltd | Insect-pathogenic fungus, spores, composition and use of same |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106490066A (en) * | 2016-10-31 | 2017-03-15 | 郑州思辩科技有限公司 | A kind of microbial environment protecting insecticide and preparation method thereof |
| GB2577250A (en) * | 2018-09-14 | 2020-03-25 | Bionema Ltd | Insect-pathogenic fungus, spores, composition and use of same |
| CN110169421A (en) * | 2019-05-20 | 2019-08-27 | 华中农业大学 | Application of the Metarhizium anisopliae in preparation prevention and treatment plant root-knot nematodes drug |
| CN110438016A (en) * | 2019-09-05 | 2019-11-12 | 江西省科学院微生物研究所 | A kind of Metarhizium anisopliae bacterial strain and its application |
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