CN105886387A - Integral-tubing packaging method for diagnostic reagent and diagnostic reagent bottle subjected to integral-tubing packaging - Google Patents
Integral-tubing packaging method for diagnostic reagent and diagnostic reagent bottle subjected to integral-tubing packaging Download PDFInfo
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- CN105886387A CN105886387A CN201610369624.XA CN201610369624A CN105886387A CN 105886387 A CN105886387 A CN 105886387A CN 201610369624 A CN201610369624 A CN 201610369624A CN 105886387 A CN105886387 A CN 105886387A
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- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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Abstract
The invention discloses an integral-tubing packaging method for diagnostic reagent and a diagnostic reagent bottle subjected to integral-tubing packaging. The packaging method comprises the following steps that the diagnostic reagent comprises two or more types of reaction reagent, no reaction reagent is not subjected to a chemical reaction, after the reaction reagent is mixed, a reaction is conducted, and the diagnosis purpose is achieved; separate package is conducted on each type of reaction reagent, and the reaction reagent is mixed through external force puncturing or centrifugation or heating. According to the integral-tubing packaging method for the diagnostic reagent and the diagnostic reagent bottle subjected to integral-tubing packaging, the diagnostic reagent is divided into two or more parts of reagent which is isolated and preserved, in the reaction process, the reaction reagent is combined together through external force puncturing or centrifugation or heating or other means, thus the situation that the diagnostic reagent loses efficacy or stability of a certain constituent is destroyed due to mixing of two or more parts of reagent before the reaction is avoided, and meanwhile operation steps are greatly simplified.
Description
Technical field
The present invention relates to a pipe packing method and the pipeization packaging diagnostic reagent bottle of a kind of diagnostic reagent.
Background technology
Existing diagnostic reagent reaction system is more complicated, such as diagnostic nucleic acid reagent, needs first by therein slow before using
Rushing the materials such as system, enzyme, primer and fluorescent probe and separately hold preservation, enzyme the most therein, primer and fluorescence are visited
Pin needs to be positioned in the low temperature environment of-20 DEG C and preserves.Each material could be mixed in use, preparation when therefore using
System is very inconvenient, in the urgent need to providing the packing method of the diagnostic reagent of a kind of simplicity.
Summary of the invention
It is an object of the invention to provide a pipe packing method and the pipeization packaging diagnostic reagent bottle of a kind of diagnostic reagent,
The present invention preserves by two parts of diagnostic reagent or many portion of reagent being kept apart, and is stung by external force during reaction again
The modes such as broken, centrifugal or heating by two parts or are partially bonded together more, thus avoid two parts or many before reaction
The mixing of portion of reagent and the diagnostic reagent that causes lost efficacy or destroyed stablizing of a certain composition, enormously simplify simultaneously
Operating procedure.
Present invention firstly provides a pipe packing method of a kind of diagnostic reagent, comprise the steps:
Described diagnostic reagent includes at least two reaction reagent, in every kind of described reaction reagent, chemical reaction does not the most occur,
Carry out reaction after the mixing of described reaction reagent and realize diagnostic purpose；
Every kind of described reaction reagent is individually packed, and is punctured by external force, be centrifuged or realize institute by the way of heating
State the mixing between reaction reagent.
In an above-mentioned pipe packing method, reaction reagent described in two or more can be simultaneously liquid reactions reagent, with
Time be solid reaction reagent or a kind of liquid reactions reagent and a kind of solid reaction reagent.
In an above-mentioned pipe packing method, the mode of the following individually packaging of employing:
Described reaction reagent is contained in same container, isolates described reaction reagent with isolating membrane.
In an above-mentioned pipe packing method, described isolating membrane can be thin film or paraffin, when using thin film, passes through external force
The mode punctured realizes the mixing between described reaction reagent, and when using paraffin, melting point of paraffin wax is about 50 DEG C, because of
This can realize the mixing between described reaction reagent by the way of heating.
In an above-mentioned pipe packing method, when at least one described reaction reagent is the reagent needing cryopreservation, use
Following 1) in-3), described reaction reagent is processed by any one mode:
1) described reaction reagent is carried out vitrification；
2) described reaction reagent is carried out lyophilization；
3) described reaction reagent is carried out gel preservation；
Low temperature in described cryopreservation refers to the temperature less than 4 DEG C；
By making the described reaction reagent needing cryopreservation can preserve with room temperature after above-mentioned process.
Described vitrification refers to when Cucumber is in the " glass transition temperature (glass less than or equal to this material
transition temperature；Tg) glass (glasses) will be formed time ", now, because molecule is almost without movable and expansion
Dissipate, therefore permanent stabilization can be kept at normal temperatures.Owing in During Vitrification in vitro, material does not crystallize, but form a kind of pole
" super freezing (super cooled) " liquid of viscous.Now, carry on as before and remain the molecular disorder as fluid characteristics,
Therefore glass is also referred to as " amorphous solid (amorphous solid) ", and it is different from real solid with this.For mesh
The front reagent needing cryopreservation and low-temperature transport, not only considerably increases cost, but also adds risk, if
Arbitrary link of cold chain goes wrong, the reliability of reagent just it cannot be guaranteed that, therefore by method for vitrification by described instead
Answering reagent to carry out vitrification, the vitreous thing obtained can be realized as room temperature and preserves and room temperature transport, and described vitreous liquid
Described reaction reagent do not had any impact.
In an above-mentioned pipe packing method, the vitreous liquid that can be selected for routine carries out described vitrification, as one-tenth glass carrier can
Using saccharide, such as Ficoll 400 and/or melezitose, protective agent can use heat shock protein and/or dehydrated protein class etc..
In an above-mentioned pipe packing method, described lyophilization, also known as sublimation drying, is to be chilled to by water-containing materials
Below freezing, make water be changed into ice, then under high vacuum, ice is changed into steam and the drying means that removes.Thing
Material can be first freezing in refrigerating plant, then is dried；But also can directly in hothouse through being evacuated rapidly and cold
Freeze.The steam that distillation generates borrows condenser to remove, and heat of vaporization required in sublimation process typically supplies with heat radiation
Give.
In an above-mentioned pipe packing method, described gelation preserves and refers to utilize the phenomenon of solution gels, to institute
State the gel that reaction reagent formed through gelation to preserve.Gelation refers to have cold-water-soluble and the most not
The material of dissolubility, when temperature improves, the aqueous solution of this material can occur gel, such as cellulose ether compound such as Methyl cellulose
Element and derivant, i.e. methocel solution raise with temperature, and viscosity is first gradually lowered, when arriving a certain temperature,
Solution viscosity steeply rises and is gelled.
In an above-mentioned pipe packing method, described diagnostic reagent is diagnostic nucleic acid reagent, as PCR-based (includes general
Logical PCR and quantitative fluorescent PCR) diagnostic nucleic acid reagent, based on constant-temperature amplification (include LAMP, RPA, PSR,
The constant-temperature amplification methods such as CPA) diagnostic nucleic acid reagent etc..As archaeal dna polymerase, primer and dNTP are contained in
In one container, PCR reaction system is contained in another independent container, such as Tris-HCl, KCl, (NH4)2SO4、
MgSO4, Tween20 and Betaine (glycine betaine).
Invention further provides a pipeization packaging diagnosis examination of the pipe packing method realizing above-mentioned diagnostic reagent
Agent bottle, it includes the first reaction tube of an open at both ends, is fastenedly connected a sealing lid in one end of described first reaction tube；
The other end at described first reaction tube is removably disposed one second reaction tube, described second reaction tube open at one end one
End seal is closed and opening end is connected with the other end of described first reaction tube；In described first reaction tube, a barrier film is set.
In an above-mentioned pipeization packaging diagnostic reagent bottle, a groove is set in described sealing lid, is used for holding described glass
Compound.
In an above-mentioned pipeization packaging diagnostic reagent bottle, at described first reaction tube and the outer tube wall of described second reaction tube
Upper it is nested with a damping ring respectively, the described membrane ruptures when being collided with by external force can be avoided；
Described barrier film uses thin film or paraffin.
In an above-mentioned pipeization packaging diagnostic reagent bottle, described first reaction tube, described second reaction tube and described sealing
Lid all uses plastic material to make.
The application in packaging diagnostic reagent of the above-mentioned pipeization packaging diagnostic reagent bottle falls within protection scope of the present invention.
The present invention preserves by two parts of diagnostic reagent or many portion of reagent being kept apart, by outward during reaction
The modes such as power punctures, centrifugal or heating bond them together, thus two parts or many part examinations before avoiding reaction
The mixing of agent and the diagnostic reagent that causes lost efficacy or destroyed stablizing of a certain composition, enormously simplify operation step simultaneously
Suddenly.Present invention also offers the pipeization packaging diagnostic reagent bottle realizing above-mentioned packing method, employing thin film or paraffin will
Two kinds of reaction reagents are kept apart, and can be positioned over by the reaction reagent after vitrification in the groove sealed in lid, it is simple to
Holding of solid reaction reagent；And on the outer tube wall of the first reaction tube and the second reaction tube, it is nested with a damping ring respectively,
Therefore the membrane ruptures when being collided with by external force can be avoided.
Accompanying drawing explanation
Fig. 1 is the result schematic diagram of the present invention one pipeization packaging diagnostic reagent bottle.
In figure, each labelling is as follows:
1 first reaction tube, 2 sealing lid, 3 second reaction tubes, 4 barrier films.
Fig. 2 is the reaction result that in the embodiment of the present invention 2, a pipeization packaging is packed with a non-pipeization.
Fig. 3 is shigella sensitivity results in the embodiment of the present invention 3, and wherein, Fig. 3 (A) is a pipeization packaging
Method detection shigella sensitivity, curve 1 represent be sample nucleic acid concentration be 4.5ng/ul, curve 2 represent be
Sample nucleic acid concentration is 2.450pg/ul, curve 3 represent be sample nucleic acid concentration be 3.45pg/ul, curve 4 represents
Be sample nucleic acid concentration be 4.4.5pg/ul, curve 5 represent be sample nucleic acid concentration be 5.450fg/ul, curve 6
Represent be sample nucleic acid concentration be 6.45fg/ul；Fig. 3 (B) is regular-PCR method electrophoresis result, wherein, band
That M represents is DNA marker 2000, band 1 represent be sample nucleic acid concentration be 4.5ng/ul, band 2 represents
Be sample nucleic acid concentration be 2.450pg/ul, band 3 represent be sample nucleic acid concentration be 3.45pg/ul, band 4
Represent be sample nucleic acid concentration be 4.5pg/ul, band 5 is negative control.
Fig. 4 is Salmeterol fluticasone propionate test kit specificity experiments result in the embodiment of the present invention 4, wherein, and curve 1 table
Show is Salmonella, and what curve 2 represented is vibrio parahaemolyticus, and what curve 3 represented is staphylococcus aureus,
That curve 4 represents is escherichia coli O157, and what curve 5 represented is shigella dysenteriae, and what curve 6 represented is single
Increasing Listerella, what curve 7 represented is Shigella sonnei, and what curve 8 represented is escherichia coli, curve 9 table
Show is shigella flexneri, and what curve 10 represented is Pseudomonas aeruginosa, and what curve 11 represented is bacillus botulinus,
What curve 12 represented is stenotrophomonas maltophilia, and what curve 13 represented is distilled water negative control.
Detailed description of the invention
Experimental technique used in following embodiment if no special instructions, is conventional method.
Material used in following embodiment, reagent etc., if no special instructions, the most commercially obtain.
Embodiment 1, one pipeization packaging diagnostic reagent bottle
As it is shown in figure 1, the present invention one pipeization packaging diagnostic reagent bottle includes the first reaction tube 1 of an open at both ends,
One end of first reaction tube 1 is fastenedly connected a sealing lid 2, arranges one and hold the recessed of solid reactants in sealing lid 2
Groove 21.The other end at the first reaction tube 1 is removably disposed one second reaction tube 3, and second reaction tube 3 one end opens
Mouth one end is closed, and the other end of opening end and the first reaction tube 1 connects.It is fastenedly connected one in the first reaction tube 1
Barrier film 4 vertically arranged with surrounding inner tubal wall.Reactant within stopping the first reaction tube 1 of barrier film 4 and second anti-
Should the reactant of pipe 3 contact in a non-operative state.
In above-described embodiment, barrier film 4 can use thin film or paraffin.
In above-described embodiment, first reaction tube the 1, second reaction tube 3 and sealing lid 2 can use transparent plastic material
Make.
In above-described embodiment, the outer tube wall of the first reaction tube 1 and the second reaction tube 3 can be nested with a damping ring respectively
5, it is used for avoiding this device to cause when being collided with by external force barrier film 4 to rupture and makes reactant react in a non-operative state.
Embodiment 2, a pipeization packaging of diagnostic nucleic acid reagent
Diagnostic nucleic acid reagent:
Reaction reagent A: need the reagent that low temperature (-20 DEG C to 4 DEG C) transports, Bst archaeal dna polymerase, four kinds of dNTP
With primer (F3:CGCTGACAATCGCTTCTCAT；B3:GTTCTTCGCTTTGGCAATGT；FIP:
CTGTCACCGAGTGCAACCACTT-AACCACACGATCTGGAGCA；BIP:
GCATCACAATGGCGCTTCCC-ACCGTTGGAGAAGTGACCTA；LF:
CTGCATTGCTGCGTCGT；LB:TAACCCGAACAGCTGGTTCT)；
Reaction reagent B: can room temperature transport liquid reactions reagent, Tris-HCl, KCl, (NH4)2SO4、MgSO4、
Tween20 and Betaine (glycine betaine).
Reaction reagent A is carried out vitrification: vitreous liquid is made up of two parts: a part is into glass carrier, belongs to saccharifying
Thing；Another part is protective agent, belongs to heat shock protein and dehydrated protein class, by reaction reagent A and vitreous liquid according to
The volume ratio mixing of 10:1, is placed in the ventilated environment of 50 DEG C 10 hours, i.e. completes vitreous process.
Vitreous thing obtained above is positioned in embodiment 1 one pipeization packaging diagnostic reagent bottle and seals the groove in lid 2
In 21, reaction reagent B is contained in the second reaction tube 3 in embodiment 1 one pipeization packaging diagnostic reagent bottle, will
First reaction tube 1 coordinates with the second reaction tube 3, and isolates with paraffin, then with sealing lid 2 sealing the
One reaction tube 1, so far, is achieved that the pipeization packaging to diagnostic nucleic acid reagent.
Storage and transport will be carried out, during use, by dropping sample under above-mentioned packaged diagnostic nucleic acid reagent room temperature
Nucleic acid on vitreous thing, gets rid of after dissolving vitreous thing at the bottom of pipe, then by reaction reagent A and reaction examination by the way of heating
Agent B mixes, and is placed in real-time transmissometer by the mixed liquor obtained, and 65 DEG C of reaction 60min, by record 650nm
Light absorption value at wavelength, every 6s monitors the turbidity of reaction tube in real time, and is depicted as curve, as in figure 2 it is shown, by this figure
It can be seen that the reaction reagent of the reaction reagent of a pipeization packaging and a non-pipeization packaging is at response time and amplification efficiency
Upper the most basically identical, there is no significant difference, it was demonstrated that after the inventive method preserves, do not affect the effective of diagnostic reagent
Property and stability.
Embodiment 3, a pipeization packaging of bacillus dysenteriae diagnostic reagent
Diagnostic nucleic acid reagent:
Reaction reagent A: need the reagent that low temperature (-20 DEG C to 4 DEG C) transports, Bst archaeal dna polymerase, four kinds of dNTP
With primer (F3:AAGTCTACCTGGCCTTCCA；B3:CCGTACGCTTCAGTACAGC；FIP:
CCGACACGCCATAGAAACGCAT-CCATGCTCGCAGAGAAACT；BIP:
CAAATGACCTCCGCACTGCCG-CCAGAGGGAGAACCAGTCC；LF:
CGGCAGTGGAGAGCTGA；LB:AAGCTATGGTCAGAAGCCGT)；
Reaction reagent B: can room temperature transport liquid reactions reagent, Tris-HCl, KCl, (NH4)2SO4、MgSO4、
Tween20 and Betaine (glycine betaine).
Reaction reagent A is carried out vitrification, and detailed process is with identical in embodiment 2.
Vitreous thing obtained above is positioned in embodiment 1 one pipeization packaging diagnostic reagent bottle and seals the groove in lid 2
In 21, reaction reagent B is contained in the second reaction tube 3 in embodiment 1 one pipeization packaging diagnostic reagent bottle, will
First reaction tube 1 coordinates with the second reaction tube 3, and isolates with paraffin, then with sealing lid 2 sealing the
One reaction tube 1, so far, is achieved that the pipeization packaging to diagnostic nucleic acid reagent.
Storage and transport will be carried out, during use, by dropping sample under above-mentioned packaged diagnostic nucleic acid reagent room temperature
Nucleic acid on vitreous thing, gets rid of after dissolving vitreous thing at the bottom of pipe, then by reaction reagent A and reaction examination by the way of heating
Agent B mixes, and is placed in real-time transmissometer by the mixed liquor obtained, and 65 DEG C of reaction 60min, by record 650nm
Light absorption value at wavelength, every 6s monitors the turbidity of reaction tube in real time, and is depicted as curve, as it is shown on figure 3, a pipe
The detection kit of packaging is 100 times of regular-PCR in sensitivity, demonstrates again that a pipeization packaging does not affect diagnosis
The effectiveness of reagent and stability.
Embodiment 4, a pipeization packaging of Salmonella diagnostic reagent
Diagnostic nucleic acid reagent:
Reaction reagent A: need the reagent that low temperature (-20 DEG C to 4 DEG C) transports, Bst archaeal dna polymerase, four kinds of dNTP
With primer (F3:GGCGATATTGGTGTTTATGGGG；B3:AACGATAAACTGGACCACGG；
FIP:GACGACTGGTACTGATCGAT-AGTTTTTCAACGTTTCCTGCGG；BIP:
CCGGTGAAATTATCGCCAC-ACAAAACCCACCGCCAGG；LF:
GACGAAAGAGCGTGGTAATTAAC；LB:GGGCAATTCGTTATTGGCGATAG)；
Reaction reagent B: can room temperature transport liquid reactions reagent, Tris-HCl, KCl, (NH4)2SO4、MgSO4、
Tween20 and Betaine (glycine betaine).
Reaction reagent A is carried out vitrification, and detailed process is with embodiment 2.
Vitreous thing obtained above is positioned in embodiment 1 one pipeization packaging diagnostic reagent bottle and seals the groove in lid 2
In 21, reaction reagent B is contained in the second reaction tube 3 in embodiment 1 one pipeization packaging diagnostic reagent bottle, will
First reaction tube 1 coordinates with the second reaction tube 3, and isolates with paraffin, then with sealing lid 2 sealing the
One reaction tube 1, so far, is achieved that the pipeization packaging to diagnostic nucleic acid reagent.
Storage and transport will be carried out, during use, by dropping sample under above-mentioned packaged diagnostic nucleic acid reagent room temperature
Nucleic acid on vitreous thing, gets rid of after dissolving vitreous thing at the bottom of pipe, then by reaction reagent A and reaction examination by the way of heating
Agent B mixes, and is placed in real-time transmissometer by the mixed liquor obtained, and 65 DEG C of reaction 60min, by record 650nm
Light absorption value at wavelength, every 6s monitors the turbidity of reaction tube in real time, and is depicted as curve, as shown in Figure 4, by this figure
It can be seen that the Salmonella diagnostic reagent through a pipeization packaging also show good result, only on specificity
Have Salmonella to there occurs positive reaction, all there is not positive reaction in other bacterium, it was demonstrated that preserves through the inventive method
After, do not affect effectiveness and the stability of diagnostic reagent.
Claims (9)
1. a pipe packing method of diagnostic reagent, comprises the steps:
Described diagnostic reagent includes at least two reaction reagent, in every kind of described reaction reagent, chemical reaction does not the most occur,
Carry out reaction after the mixing of described reaction reagent and realize diagnostic purpose；
Every kind of described reaction reagent is individually packed, and is punctured by external force, be centrifuged or realize institute by the way of heating
State the mixing between reaction reagent.
A pipe packing method the most according to claim 1, it is characterised in that: use following individually packaging
Mode:
Described reaction reagent is contained in same container, isolates described reaction reagent with isolating membrane.
A pipe packing method the most according to claim 2, it is characterised in that: described isolating membrane be thin film or
Paraffin.
4. according to the pipe packing method according to any one of claim 1-3, it is characterised in that: at least one institute
State reaction reagent when being the reagent needing cryopreservation, use following 1)-3) in any one mode to described reaction reagent
Process:
1) described reaction reagent is carried out vitrification；
2) described reaction reagent is carried out lyophilization；
3) described reaction reagent is carried out gel preservation.
Described low temperature refers to the temperature less than 4 DEG C.
5. according to a described pipe packing method arbitrary in claim 1-4, it is characterised in that: described diagnostic reagent
For diagnostic nucleic acid reagent.
6. realize a pipeization packaging diagnosis of a pipe packing method of diagnostic reagent according to any one of claim 1-5
Reagent bottle, it is characterised in that: it includes the first reaction tube of an open at both ends, tight in one end of described first reaction tube
It is solidly connected a sealing lid；The other end at described first reaction tube is removably disposed one second reaction tube, and described second
Reaction tube open at one end one end is closed and opening end is connected with the other end of described first reaction tube；React described first
One barrier film is set in pipe.
A pipeization packaging diagnostic reagent bottle the most according to claim 6, it is characterised in that: seal lid described
One groove is inside set.
8. according to the pipeization packaging diagnostic reagent bottle described in claim 6 or 7, it is characterised in that: described the
A damping ring it is nested with respectively on the outer tube wall of one reaction tube and described second reaction tube；
Described barrier film uses thin film or paraffin.
9. pipeization packaging diagnostic reagent bottle application in packaging diagnostic reagent according to any one of claim 6-8.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610369624.XA CN105886387B (en) | 2016-05-30 | 2016-05-30 | One pipe packing method of diagnostic reagent and a pipeization pack diagnostic reagent bottle |
| PCT/CN2016/085076 WO2017206194A1 (en) | 2016-05-30 | 2016-06-07 | One-tube packaging method for diagnostic reagent and bottle for one-tube packaged diagnostic reagent |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610369624.XA CN105886387B (en) | 2016-05-30 | 2016-05-30 | One pipe packing method of diagnostic reagent and a pipeization pack diagnostic reagent bottle |
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| CN105886387A true CN105886387A (en) | 2016-08-24 |
| CN105886387B CN105886387B (en) | 2018-08-07 |
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| WO (1) | WO2017206194A1 (en) |
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| CN108531377A (en) * | 2018-06-26 | 2018-09-14 | 广东省第二人民医院（广东省卫生应急医院） | It is a kind of can pre-filled reagent diaphragm type nucleic acid amplification airtight reactor tube |
| CN112516092A (en) * | 2020-12-14 | 2021-03-19 | 上海简逸生物科技有限公司 | Freeze-dried preparation manufacturing process with co-existing redissolution and freeze-dried spheres and freeze-dried preparation |
| CN112535478A (en) * | 2020-12-29 | 2021-03-23 | 江西金域医学检验所有限公司 | Multifunctional vacuum blood collection tube |
| CN113736868A (en) * | 2021-09-16 | 2021-12-03 | 广州生凌医疗科技有限公司 | RNA nucleic acid detection reagent storage method, kit and use method |
| CN114657236A (en) * | 2020-12-23 | 2022-06-24 | 广州达安基因股份有限公司 | Method for separating nucleic acid amplification system and separating agent |
| CN117025379A (en) * | 2023-10-09 | 2023-11-10 | 迪飞医学科技(南京)有限公司 | RAPID isothermal amplification nucleic acid detection device and detection method |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109355405B (en) * | 2018-08-30 | 2022-10-25 | 华南理工大学 | Primer, kit and method for detecting vibrio parahaemolyticus by PSR isothermal amplification reaction |
| CN109355407B (en) * | 2018-09-26 | 2021-09-21 | 华南理工大学 | Primer, kit and method for detecting pseudomonas aeruginosa through PSR isothermal amplification reaction |
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| CN114657236B (en) * | 2020-12-23 | 2024-06-18 | 广州达安基因股份有限公司 | Method for separating nucleic acid amplification system and separating agent |
| CN112535478A (en) * | 2020-12-29 | 2021-03-23 | 江西金域医学检验所有限公司 | Multifunctional vacuum blood collection tube |
| CN113736868A (en) * | 2021-09-16 | 2021-12-03 | 广州生凌医疗科技有限公司 | RNA nucleic acid detection reagent storage method, kit and use method |
| CN113736868B (en) * | 2021-09-16 | 2023-01-24 | 广州生凌医疗科技有限公司 | RNA nucleic acid detection reagent storage method, kit and use method |
| CN117025379A (en) * | 2023-10-09 | 2023-11-10 | 迪飞医学科技(南京)有限公司 | RAPID isothermal amplification nucleic acid detection device and detection method |
| CN117025379B (en) * | 2023-10-09 | 2023-12-15 | 迪飞医学科技(南京)有限公司 | RAPID isothermal amplification nucleic acid detection device and detection method |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2017206194A1 (en) | 2017-12-07 |
| CN105886387B (en) | 2018-08-07 |
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