CN105866113A - Preparation method of urinalysis reagent kit with phenol nucleus amino acid and derivative thereof - Google Patents
Preparation method of urinalysis reagent kit with phenol nucleus amino acid and derivative thereof Download PDFInfo
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- CN105866113A CN105866113A CN201610236141.2A CN201610236141A CN105866113A CN 105866113 A CN105866113 A CN 105866113A CN 201610236141 A CN201610236141 A CN 201610236141A CN 105866113 A CN105866113 A CN 105866113A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
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- G01N21/82—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a precipitate or turbidity
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
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Abstract
A urinalysis reagent kit with phenol nucleus amino acid and a derivative thereof is composed of a contrast reagent and a test reagent. Reagent ingredients include mercurous nitrate, nickel nitrate, mercury sulfate, an emulsifier and corresponding solvents, namely, nitric acid and sulfuric acid. The contrast reagent and the test reagent are both prepared by mixing a solution A, a solution B, a solution C and a solution D at the volume ratio of 3:5:1:1, wherein the solution A is obtained by dissolving mercury sulfate in a sulfuric acid solution, the solution B is obtained by dissolving nickel nitrate in a nitric acid solution, the solution C is obtained by dissolving mercurous nitrate in a nitric acid solution, and the solution D is obtained by dissolving the emulsifier in a nitric acid solution. When the urinalysis reagent kit with phenol nucleus amino acid and the derivative thereof is used for testing confirmed malignant tumor patients and nonmalignant tumor patients, compared with a pathological diagnosis result, in all detection indexes, the sensitiveness is 96%, the specificity is 99%, the accuracy rate is 98%, the false positive rate is 1%, the false negative rate is 3.6%, the positive result predicted value is 98.2%, and the negative result predicted value is 98.1%.
Description
Technical field
The present invention relates to the preparation method of screening for cancer kit, especially a kind of phenol core amino acid and derivative urine examination thereof
The preparation method of kit.
Background technology
The early detection of cancer, especially naturally grows the preclinical phase discovery of history, is to reduce tumor incidence and cancer in tumour
The key of the disease death rate.In urine, phenol core amino acid and derivative thereof belong to cancer rationality metabolite, than other tumour marks
Will thing occurs earlier, and such detection reagent becomes the focus of existing market exploitation.In recent years, domestic and international correlation technique patent is equal
Innovate on the basis of millon reagent principle, have certain effect in the application, but, a large amount of it was verified that there is also not
In place of foot.First, false positive rate is the highest, reaches 13%, is not suitable for generaI investigation and the examination of cancer.Reason is the highly sensitive of reagent,
Detection limit reaches the 100mg/L(gauge with tyrosine), range of normal value clinically is less than 250mg/L；Second, reaction speed
Degree is slow, and (the urine specimen test experience of MEC is to concentrate to carry out, and the temperature of urine specimen is low, under the solubility of tyrosine etc.
Fall), cause the extraneous oxide oxidation to reaction system, deepen the color of reaction system, make reading become big；3rd, colorimetric card
Can not accomplish standard and generally applicable, the color of different urine specimen itself is different, though the detection substrate of same concentrations
The color of its reaction result is variant, and when being especially in critical value, the negative or positive is difficult to differentiate between.These weak points affect phase
Close the popularization and application of technical products.
The purpose of the present invention is to propose to a kind of new phenol core amino acid and derivative urinalysis reagent box thereof, be simultaneously introduced new component,
To improve the stability of reagent, solubilising and enhanced sensitivity.
Summary of the invention
The purpose of the present invention is aiming at defect and the deficiency of prior art, it is provided that a kind of good stability, the degree of accuracy are high
Phenol core amino acid and the preparation method of derivative urinalysis reagent box thereof.
The phenol core amino acid of the present invention and derivative urinalysis reagent box thereof, be made up of contrast agents and test agent, reagent
Composition include mercurous nitrate (Hg2(NO3)2.2H2O), nickel nitrate (Ni (NO3)2.6H2O), mercuric sulfate (HgSO4) and polyoxyethylene nonylphenol ether
And corresponding solvent nitric acid (HNO3) and sulfuric acid (H2SO4), its preparation method, comprise the following steps:
1, the preparation of contrast agents:
A1Prepared by solution: weigh 75-125g HgSO4It is dissolved in the sulfuric acid solution of 1500ml 3-7M；
B1Prepared by solution: weigh 250-320g Ni (NO3)2.6H2O is dissolved in the salpeter solution of 2500ml 5-10M；
C1Prepared by solution: weigh 11g Hg2(NO3)2.2H2O is dissolved in the salpeter solution of 500ml 5-10M；
D1Prepared by solution: weigh 25-45g polyoxyethylene nonylphenol ether and be dissolved in the salpeter solution of 500ml 5-10M；
By above four kinds of liquid A by volume1Solution: B1Solution: C1Solution: D1Solution=3:5:1:1 mixing, i.e. obtains
Contrast agents.
2, the preparation of test agent:
A2Prepared by liquid: weigh 75-125g HgSO4It is dissolved in the sulfuric acid solution of 1500ml 3-7M；
B2Prepared by liquid: weigh 250-320g Ni (NO3)2.6H2O is dissolved in the salpeter solution of 2500ml 5-10M；
C2Prepared by solution: weigh 87-156g Hg2(NO3)2.2H2O is dissolved in the salpeter solution of 500ml 5-10M；
D2Prepared by solution: weigh 25-45g polyoxyethylene nonylphenol ether and be dissolved in the salpeter solution of 500ml 5-10M；
By above four kinds of liquid A by volume2Solution: B2Solution: C2Solution: D2Solution=3:5:1:1 mixing, i.e. obtains
Test agent.
The phenol core amino acid of the present invention and derivative urinalysis reagent box thereof, when malignant tumour examination and auxiliary diagnosis,
Respectively take urina sanguinis 3ml, be respectively dropped into contrast agents and each 0.5ml of test agent of this kit, shake up, stand 1-3 minute, observe
Color changes.If two colors are identical, white/yellow precipitation or light red precipitation person the most all occur for feminine gender；If two
Color is all red and the color of test agent reaction is deeper, then be positive.
The phenol core amino acid of the present invention and derivative urinalysis reagent box thereof, reagent, can be real in treatment when curative effect monitoring
Execute and front and back detect.As being still positive after treatment, then curative effect is inconspicuous；As before treating for positive reaction, for turning out cloudy after treatment
Property reaction, then treatment is effectively.
The phenol core amino acid of the present invention and derivative urinalysis reagent box thereof, reagent, when state of illness monitoring, can use above-mentioned side
Method periodic detection, in the process, as occurred, the color of precipitation is gradually deepened, or is transferred to positive reaction by negative reaction, then carry
Show tumor recurrence or transfer.
The phenol core amino acid of the present invention and derivative urinalysis reagent box thereof, to the 965 example malignant tumor patients made a definite diagnosis and
Non-malignant tumors patient test, wherein malignant tumour 337 example, non-malignant tumors 628 example, and result is: reactor 341
Example, negative reaction person 624 example.After compareing with pathological diagnosis result, false positive 6 example, false negative 12 example, positive 325 examples, negative
622 examples.Every Testing index is: susceptibility 96%, specific 99%, and accuracy rate 98%, false positive rate 1%, false negative rate 3.6%, sun
Property prediction of result value 98.2%, negative findings predicted value 98.1%.
The phenol core amino acid of the present invention and derivative urinalysis reagent box thereof, be applied to curative effect monitoring, 57 examples made a definite diagnosis evil
Property tumour 65 chemotherapy treatments carry out therapeutic evaluation, the index of employing be complete incidence graph, part alleviate, stable and progress.Inspection
Surveying result is that 19 chemotherapy treatments are complete incidence graph and part is alleviated, (clinical evaluation, complete incidence graph compared with same period clinical evaluation
The chemotherapy treatment alleviated with part is 21), the coincidence rate of this Evaluation is 90%.
93 examples have been treated the malignant tumor patient left hospital by the phenol core amino acid of the present invention and derivative urinalysis reagent box thereof
The state of an illness carrying out a year and a half follows the tracks of observation, and 16 examples gradually show positive reaction, the recurrence of prompting tumour or transfer.With facing of the same period
Bed data is compared, and the person of making a definite diagnosis is 14 examples, and accuracy rate is 87.5%.
Detailed description of the invention
Embodiment 1: a kind of phenol core amino acid and derivative urinalysis reagent box thereof, contrast agents and test agent are prepared respectively
5000ml:
Prepared by contrast agents:
A1Prepared by liquid: weigh 75g HgSO4It is dissolved in the sulfuric acid solution of 1500ml 7M；
B1Prepared by liquid: weigh 250gNi (NO3)2.6H2O is dissolved in the salpeter solution of 2500ml 5M；
C1Prepared by solution: weigh 11g Hg2(NO3)2.2H2O is dissolved in the salpeter solution of 500ml 5M；
D1Prepared by solution: weigh 25g polyoxyethylene nonylphenol ether and be dissolved in the salpeter solution of 500ml 5M；
By above four kinds of liquid A by volume1Solution: B1Solution: C1Solution: D1Solution=3:5:1:1 mixing, i.e. obtains
Contrast agents test agent.
Prepared by test agent: compared with contrast agents, only C2Solution preparation difference, other A1With A2、B1With B2、D1With D2
Identical, C2Prepared by solution: weigh 87g Hg2(NO3)2.2H2O is dissolved in the salpeter solution of 500ml 5M；Above four kinds of liquid are pressed body
Amass and compare A2Solution: B2Solution: C2Solution: D2Solution=3:5:1:1 mixing, i.e. obtains test agent.
Embodiment 2: a kind of phenol core amino acid and derivative urinalysis reagent box thereof, contrast agents and test agent are prepared respectively
5000ml:
Prepared by contrast agents:
A1Prepared by liquid: weigh 125g HgSO4It is dissolved in the sulfuric acid solution of 1500ml 3M；
B1Prepared by liquid: weigh 320gNi (NO3)2.6H2O is dissolved in the salpeter solution of 2500ml 10M；
C1Prepared by solution: weigh 11g Hg2(NO3)2.2H2O is dissolved in the salpeter solution of 500ml 10M；
D1Prepared by solution: weigh 45g polyoxyethylene nonylphenol ether and be dissolved in the salpeter solution of 500ml 10M；
By above four kinds of liquid A by volume1Solution: B1Solution: C1Solution: D1Solution=3:5:1:1 mixing, i.e. obtains
Contrast agents test agent.
Prepared by test agent: compared with contrast agents, only C2Solution preparation difference, other A1With A2、B1With B2、D1With D2
Identical, C2Prepared by solution: weigh 156g Hg2(NO3)2.2H2O is dissolved in the salpeter solution of 500ml 10M；Above four kinds of liquid are pressed
Volume ratio A2Solution: B2Solution: C2Solution: D2Solution=3:5:1:1 mixing, i.e. obtains test agent.
Embodiment 3: a kind of phenol core amino acid and derivative urinalysis reagent box thereof, contrast agents and test agent are prepared respectively
5000ml:
Prepared by contrast agents:
A1Prepared by liquid: weigh 95g HgSO4It is dissolved in the sulfuric acid solution of 1500ml 5M；
B1Prepared by liquid: weigh 300gNi (NO3)2.6H2O is dissolved in the salpeter solution of 2500ml 8M；
C1Prepared by solution: weigh 11g Hg2(NO3)2.2H2O is dissolved in the salpeter solution of 500ml 8M；
D1Prepared by solution: weigh 30g polyoxyethylene nonylphenol ether and be dissolved in the salpeter solution of 500ml 8M；
By above four kinds of liquid A by volume1Solution: B1Solution: C1Solution: D1Solution=3:5:1:1 mixing, i.e. obtains
Contrast agents test agent.
Prepared by test agent: compared with contrast agents, only C2Solution preparation difference, other A1With A2、B1With B2、D1With D2
Identical, C2Prepared by solution: weigh 126g Hg2(NO3)2.2H2O is dissolved in the salpeter solution of 500ml 8M；Above four kinds of liquid are pressed
Volume ratio A2Solution: B2Solution: C2Solution: D2Solution=3:5:1:1 mixing, i.e. obtains test agent.
Claims (1)
1. a phenol core amino acid and the preparation method of derivative urinalysis reagent box thereof, it is characterised in that: it comprises the following steps:
(1), the preparation of contrast agents:
A1Prepared by solution: weigh 75-125g HgSO4It is dissolved in the sulfuric acid solution of 1500ml 3-7M；
B1Prepared by solution: weigh 250-320g Ni (NO3)2.6H2O is dissolved in the salpeter solution of 2500ml 5-10M；
C1Prepared by solution: weigh 11g Hg2(NO3)2.2H2O is dissolved in the salpeter solution of 500ml 5-10M；
D1Prepared by solution: weigh 25-45g polyoxyethylene nonylphenol ether and be dissolved in the salpeter solution of 500ml 5-10M；
By above four kinds of liquid A by volume1Solution: B1Solution: C1Solution: D1Solution=3:5:1:1 mixing, i.e. obtains
Contrast agents；
(2), the preparation of test agent:
A2Prepared by liquid: weigh 75-125g HgSO4It is dissolved in the sulfuric acid solution of 1500ml 3-7M；
B2Prepared by liquid: weigh 250-320g Ni (NO3)2.6H2O is dissolved in the salpeter solution of 2500ml 5-10M；
C2Prepared by solution: weigh 87-156g Hg2(NO3)2.2H2O is dissolved in the salpeter solution of 500ml 5-10M；
D2Prepared by solution: weigh 25-45g polyoxyethylene nonylphenol ether and be dissolved in the salpeter solution of 500ml 5-10M；
By above four kinds of liquid A by volume2Solution: B2Solution: C2Solution: D2Solution=3:5:1:1 mixing, i.e. obtains
Test agent.
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| CN201610236141.2A CN105866113A (en) | 2016-04-18 | 2016-04-18 | Preparation method of urinalysis reagent kit with phenol nucleus amino acid and derivative thereof |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107167471A (en) * | 2017-06-26 | 2017-09-15 | 青岛浩铂生物科技有限公司 | A kind of preparation method of the reagent of the single hydroxyl phenol derivatives of utilization nitration reaction detection |
| CN109406502A (en) * | 2018-10-22 | 2019-03-01 | 南京邮电大学 | A kind of detection reagent for the amino acid solution containing phenolic group |
| CN114088703A (en) * | 2020-08-24 | 2022-02-25 | 台湾永光化学工业股份有限公司 | Quick screening sleeve set and method for detecting activity of aluminum anode dye liquor |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107167471A (en) * | 2017-06-26 | 2017-09-15 | 青岛浩铂生物科技有限公司 | A kind of preparation method of the reagent of the single hydroxyl phenol derivatives of utilization nitration reaction detection |
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| CN109406502A (en) * | 2018-10-22 | 2019-03-01 | 南京邮电大学 | A kind of detection reagent for the amino acid solution containing phenolic group |
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| CN114088703A (en) * | 2020-08-24 | 2022-02-25 | 台湾永光化学工业股份有限公司 | Quick screening sleeve set and method for detecting activity of aluminum anode dye liquor |
| CN114088703B (en) * | 2020-08-24 | 2024-04-02 | 台湾永光化学工业股份有限公司 | Quick screen set and method for detecting activity of aluminum anode dye liquor |
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Application publication date: 20160817 |