CN105750324B - A method of repairing DDT, polycyclic aromatic hydrocarbon composite pollution soil - Google Patents
A method of repairing DDT, polycyclic aromatic hydrocarbon composite pollution soil Download PDFInfo
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- CN105750324B CN105750324B CN201610129260.8A CN201610129260A CN105750324B CN 105750324 B CN105750324 B CN 105750324B CN 201610129260 A CN201610129260 A CN 201610129260A CN 105750324 B CN105750324 B CN 105750324B
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- aromatic hydrocarbon
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
- B09C1/105—Reclamation of contaminated soil microbiologically, biologically or by using enzymes using fungi or plants
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C2101/00—In situ
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- Engineering & Computer Science (AREA)
- Mycology (AREA)
- Soil Sciences (AREA)
- Environmental & Geological Engineering (AREA)
- Health & Medical Sciences (AREA)
- Botany (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Processing Of Solid Wastes (AREA)
Abstract
A method of repairing DDT, polycyclic aromatic hydrocarbon composite pollution soil, it is related to a kind of method of repairing polluted soil, the method uses the form of in-situ immobilization to DDT, polycyclic aromatic hydrocarbon composite pollution soil, applies mixed surfactant in the 0-20cm topsoil of contaminated soil；Dosage is 70-100mg/kg soil, and is mixed, and Methylotrophic bacillus is then inoculated with（Bacillus methylotrophicus）500 ml/m of bacterium solution2；Field capacity DDT, the content of polycyclic aromatic hydrocarbon in 50-60%, periodic monitoring soil are kept, aforesaid operations can be repeated according to monitoring result after 1 month, until the DDT, polycyclic aromatic hydrocarbon content in soil reach safety of soil environment standard；This method is of great significance to soil DDT, polycyclic aromatic hydrocarbons contaminated prevention and treatment and crop production safety, to the improvement and reparation of such soil.
Description
Technical field
The present invention relates to a kind of methods of repairing polluted soil, multiple more particularly to a kind of reparation DDT, polycyclic aromatic hydrocarbon
The method for closing contaminated soil.
Background technique
In recent years, China's soil contamination problem is on the rise, and Environmental Quality of Cultivated Land constantly declines, pollutant in agricultural land soil
Have become a big focus of attention in Environmental security field by agricultural product bring potential risk.With country and the public couple
The raising of food-safety problem attention rate, for rapid test paper environment by more and more extensive concern, soil is the core of environment
Medium, soil pollution have catholicity and seriousness, the organic pollutant in soil（Such as pesticide, polycyclic aromatic hydrocarbon, Polychlorinated biphenyls
Deng）The normal operation that soil-plant system can be destroyed is coerced the mankind and biological by directly exposed or food chain transmitting and is good for
Health.Therefore, it is badly in need of exploiting economy, the practical technique that efficient, safety soil Fl is alleviated and repaired, to ensure soil-
Botanical system ecological environment security realizes the sustainable utilization of Agricultural Clean and land resource.
For how to be repaired and recycled, restore it by pesticide DDT-polycyclic aromatic hydrocarbon composite pollution soil
Production capacity, improves its environmental functional, and the market demand of this respect is very huge and urgent.The present invention is to utilize degradation
Bacterium-mixed surfactant joint repairs DDT and polycyclic aromatic hydrocarbon composite pollution soil, is a low consumption, efficiently handles skill
Art, using DDT and polycyclic aromatic hydrocarbon-degrading bacteria to organic pollutant DDT, the degradation of polycyclic aromatic hydrocarbon, in combination with mixed
Solubilization of the surfactant to organic contaminants in soil is closed, enhances soil pollutant in the solubility of water phase, raising has
The bioavailability of machine pollutant has small investment, at low cost, easy to operate and can be big to improve soil remediation efficiency
The features such as area is promoted.
Summary of the invention
The purpose of the present invention is to provide a kind of reparation DDT, the method for polycyclic aromatic hydrocarbon composite pollution soil, this method
Combined using DDT, polycyclic aromatic hydrocarbon efficient degrading bacteria and mixed surfactant and repairs DDT, polycyclic aromatic hydrocarbon composite pollution
Soil；Have to soil DDT, polycyclic aromatic hydrocarbons contaminated prevention and treatment and crop production safety, to the improvement and reparation of such soil
It is significant.
The purpose of the present invention is what is be achieved through the following technical solutions：
A method of DDT, polycyclic aromatic hydrocarbon composite pollution soil being repaired, the method includes following procedure：
The form that in-situ immobilization is used to DDT, polycyclic aromatic hydrocarbon composite pollution soil, in the 0-20cm table of contaminated soil
Mixed surfactant is applied in layer soil；Dosage is 70-100mg/kg soil, and is mixed, and Methylotrophic bud is then inoculated with
Spore bacillus（Bacillus methylotrophicus）500 ml/m of bacterium solution2；It keeps field capacity in 50-60%, periodically supervises
DDT, the content of polycyclic aromatic hydrocarbon in soil are surveyed, aforesaid operations can be repeated according to monitoring result after 1 month, until the drop in soil
DDT, polycyclic aromatic hydrocarbon content reach safety of soil environment standard；The DDT and polycyclic aromatic hydrocarbon efficient degrading bacteria bacterium solution used be
Methylotrophic bacillus（Bacillus methylotrophicus）Bacterium solution, wherein the Colony Forming Unit of bacterium be 1 ×
108It is every milliliter a；The mixed surfactant used is neopelex（SDBS）And tween（Tween-80）, mix
Composition and division in a proportion example is 2:3.
A kind of reparation DDT, the method for polycyclic aromatic hydrocarbon composite pollution soil, the mixed surfactant make
Dosage is more suitable in 40-100mg/kg.
Advantages of the present invention is with effect：
It is of the present invention to combine reparation DDT, polycyclic aromatic hydrocarbon composite pollution with mixed surfactant by degradation bacteria
The method of soil, using degradation bacteria to the degradation of DDT and polycyclic aromatic hydrocarbon, in combination with mixed surfactant to dirt
The solubilization of object is contaminated, and then realizes the purpose of DDT and polycyclic aromatic hydrocarbon in removal soil, there is low in cost, operation letter
Advantage single, repairing effect is good, has most important theories to soil DDT, polycyclic aromatic hydrocarbons contaminated prevention and treatment and crop production safety
And realistic meaning, improvement and reparation for such soil of China have great importance.
Detailed description of the invention
Fig. 1 is the residual of mixed surfactant with DDT in soil after strain-combined handle 1 month, 3 months, 5 months
Situation；
Fig. 2 is the residual of mixed surfactant with PAHs in soil after strain-combined handle 1 month, 3 months, 5 months
Situation.
Specific embodiment
The following describes the present invention in detail with reference to examples.
Embodiment：Field plot experiment
Specific implementation step is as follows：
1) degradation germ liquid is configured, used DDT and polycyclic aromatic hydrocarbon-degrading bacteria are Methylotrophic bacillus
（Bacillus methylotrophicus）, the Colony Forming Unit of bacterium solution is 1 × 108It is every milliliter a.
2) mixed surfactant is configured, used mixed surfactant is neopelex（SDBS）
And tween（Tween-80）Mixture, mixed proportion 2:3.
3）It tests in the greenhouse that place is Shenyang City Shenbeixin District industrialized agriculture, using field experiment mode, hillslope processes
It is set as 1m*1m, the content of DDT is 47.94 μ g/kg, polycyclic aromatic hydrocarbon content 0.69mg/kg in soil.In contaminated soil
0-20cm topsoil in apply various dose mixed surfactant（Dosage is respectively 40,70,100,200,
300mg/kg soil）And mix, then it is inoculated with Methylotrophic bacillus（Bacillus methylotrophicus）Bacterium solution
500 ml/m2.Keep field capacity in 50-60%, respectively 1 month, 3 months, 5 months periodic monitorings after experiment starts
DDT, the content of polycyclic aromatic hydrocarbon in soil.
Mixed surfactant and after the strain-combined processing of degradation bacteria 1 month, 3 months, 5 months in soil DDT residual
Situation is as shown in Figure 1.Mixed surfactant is with after strain-combined processing 1 month, and the degradation rate of DDT is in 38.23- in soil
In 63.53% range, the DDT degradation rate highest that wherein mixed surfactant H70 is handled, up to 63.53%.With the processing time
Increase（1,3,5 months）, the degradation rate of mixed surfactant and DDT in the soil of strain-combined processing gradually decreases,
After processing 5 months, the DDT degradation rate highest of H70, up to 63.98%.After handling 5 months in soil in terms of the residual condition of DDT,
The DDT degradation rate of H40, H70, H100 processing is respectively 55.85%, 63.98% and 54.07%, and mixed surfactant usage amount exists
40-100mg/kg is more suitable.
The residual feelings of Fig. 1 mixed surfactant and DDT in soil after strain-combined handle 1 month, 3 months, 5 months
Condition.
Mixed surfactant and after the strain-combined processing of degradation bacteria 1 month, 3 months, 5 months in soil PAHs residual
Situation is as shown in Figure 2.Mixed surfactant is with after strain-combined processing 1 month, and the degradation rate of PAHs is in 24.90- in soil
In 38.54% range, the PAHs degradation rate highest that wherein mixed surfactant H100 is handled, up to 38.54%.When with processing
Between increase（1,3,5 months）, the degradation rate of mixed surfactant and PAHs in the soil of strain-combined processing gradually decreases,
After processing 5 months, the PAHs degradation rate highest of H100, up to 41.06%.After processing 5 months in soil PAHs residual feelings
Condition sees that the DDT degradation rate of H70, H100, H200 processing is respectively 35.24%, 41.06% and 39.58%, and mixed surfactant makes
Dosage is more suitable in 70-200mg/kg.
The residual feelings of Fig. 2 mixed surfactant and PAHs in soil after strain-combined handle 1 month, 3 months, 5 months
Condition.
Comprehensively consider mixed surfactant and strain-combined processing to DDT in soil and the PAHs repairing effect polluted and
Cost, the final usage amount for determining mixed surfactant are more suitable in 70-100 mg/kg.In the present embodiment, 5 months
After the reparation phase, mixed surfactant is in 70-100 mg/kg dosage range, and mixed surfactant and degradation bacteria are to soil
The degradation rate of middle DDT and PAHs can respectively reach 54.07-63.98% and 35.24-41.06%.
Claims (1)
1. a kind of reparation DDT, the method for polycyclic aromatic hydrocarbon composite pollution soil, which is characterized in that the method includes following mistakes
Journey：
The form that in-situ immobilization is used to DDT, polycyclic aromatic hydrocarbon composite pollution soil, in the 0-20cm topsoil of contaminated soil
Middle application mixed surfactant；Dosage is 70-100mg/kg soil, and is mixed, and Methylotrophic gemma bar is then inoculated with
Bacterium（Bacillus methylotrophicus）500 ml/m of bacterium solution2；Keep field capacity in 50-60%, periodic monitoring soil
DDT, the content of polycyclic aromatic hydrocarbon in earth can repeat aforesaid operations according to monitoring result after 1 month, until DDT in soil,
Polycyclic aromatic hydrocarbon content reaches safety of soil environment standard；Wherein the Colony Forming Unit of Methylotrophic bacillus bacterium solution is 1
×108It is every milliliter a；The mixed surfactant used is neopelex（SDBS）With tween Tween-80, mix
Composition and division in a proportion example is 2:3.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106734187A (en) * | 2017-01-05 | 2017-05-31 | 沈阳大学 | A kind of method for strengthening combine d bioremediation polycyclic aromatic hydrocarbon pollution |
| CN106825033A (en) * | 2017-01-05 | 2017-06-13 | 沈阳大学 | A kind of biological reinforced method for repairing DDT polycyclic aromatic hydrocarbon composite pollution soil |
| CN108941177B (en) * | 2018-09-10 | 2019-05-07 | 江西夏氏春秋环境股份有限公司 | It is a kind of for repairing the eluent and its application method of Cu, Pb, As combined contamination soil |
| CN110090859A (en) * | 2019-04-12 | 2019-08-06 | 沈阳大学 | A kind of method that plant enhancement microbiological repairs PAHs pollution in agricultural land soil |
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Effective date of registration: 20201223 Address after: People's Government of Jinshan Town, building 15, Qingshan langu community, Tangquan Road, Jinshan Town, Yuanbao District, Dandong City, Liaoning Province Patentee after: Dandong Jinshan Investment Construction Development Co.,Ltd. Address before: 110044 Liaoning Province, Shenyang City District wanghuanan Street No. 21 Patentee before: SHENYANG University |
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