CN105749250A - Application of antibacterial peptides of wood frogs to preparing medicines for treating onychomycosis - Google Patents

Abstract

The invention discloses a new application of the Rana antibacterial peptide, specifically the application of the Rana antibacterial peptide in the preparation of medicine for treating onychomycosis. The use method is simple, the effective rate and the cure rate are high, and it is suitable for different age groups, has no side effect, and has a low recurrence rate.

Description

Application of Rana antibacterial peptide in the preparation of medicine for treating onychomycosis

technical field

The invention relates to a new medical application of the Rana antibacterial peptide, in particular to its application in the preparation of medicine for treating onychomycosis.

Background technique

Onychomycosis, commonly known as "onychomycosis", refers to a disease caused by dermatophytes invading the nail plate or under the nail. Onychomycosis is often caused by various fungi such as Trichophyton rubrum, Trichophyton mentagrophytes, and Epidermophyton flocculum. A few are caused by other filamentous fungi, yeast-like bacteria and saccharomyces, and occasionally it can also be caused by sporozoites, Fusarium and Aspergillus terrescens, etc., mostly seen in malnourished nails.

Onychomycosis is the most stubborn and intractable type of dermatophytosis. It can be taken orally, externally, or surgically. The growth rate of fingernails and toenails is different. It takes 100 days to completely replace a new nail, while it takes about 300 days for toenails, so you must be patient in the treatment of onychomycosis. Currently commonly used treatments include:

(1) Surgical nail removal

It is suitable for single nail, under local anesthesia, remove the affected nail. This method has a large wound, bleeding, and easy to cause infection. After the operation, you will generally feel more pain, and it is easy to re-infect and relapse, so it is rarely used in clinical practice. Patients with heart disease, high blood pressure, and diabetes are not suitable for surgical treatment.

(2) Oral medicine

Suitable for multiple nails. For example, take 250 mg terbinafine tablets orally daily for 6 to 12 weeks; itraconazole 400 mg/day, take 7 days and rest for 21 days as a course of treatment for 3 to 6 courses; 1 to 2 weeks a week Take fluconazole 150mg every time for more than 4 months. More than 80% of onychomycosis and onychomycosis can be cured. However, because this type of drug must reach the deck where the fungus is parasitic, it can exert its antibacterial effect, the dosage is large, and the medication time is long, so the adverse drug reactions should be monitored regularly.

(3) External therapy

According to different medicaments, the main methods are topical application and packet cutting:

(1) Use a nail file (non-professionals should not use blades to avoid bleeding and infection of other diseases) to thin irregular nails, and the nail grinding cycle is preferably once every 2 days;

(2) Topically apply 30% glacial acetic acid or 10% glacial acetic acid to soak the diseased nails once a day for more than 3 to 6 months. If the sick nail is thinned before applying the medicine, the curative effect will be better. Pay attention to protect the skin around the nail with mild ointment before applying the nail;

(3) Use terbinafine tincture, amorolfine A liniment, ciclopirox ketone A varnish and other antifungal topical drugs for topical application.

It can be seen that the current treatment methods mostly have disadvantages such as long medication time, complicated treatment methods, unstable drug efficacy, high recurrence rate, susceptibility to infection, and toxic and side effects.

Contents of the invention

In order to solve the above-mentioned problems in the prior art, the present invention has carried out a large number of experiments in this field, and found that the Rana antibacterial peptide can be used to prepare a drug for treating onychomycosis.

Antimicrobial peptides (AMPs) are an important part of the internal defense system of organisms. Antimicrobial peptides have the ability to resist Gram-positive bacteria, Gram-negative bacteria, fungi, coated viruses, protozoa and drug-resistant bacteria, and are currently used in the field of food preservation boxes and medicine.

Rana is an amphibian, and its habitat environment makes its skin have active substances with complex functions. In the Chinese patent application with publication number CN1216768A, a method for preparing Rana antimicrobial peptides is disclosed.

On the basis of preparing the Rana antibacterial peptide, the present invention discovers a new application of the Rana antibacterial peptide, that is, it is used to prepare medicine for treating onychomycosis.

The Rana antimicrobial peptide of the present invention can be obtained by purchase or preparation. The preparation method includes but not limited to extraction method, ultrasonic extraction method, microwave extraction method and the like. The Rana antimicrobial peptide obtained by any method can be used to prepare medicine for treating onychomycosis.

The specific preparation method of the Rana antimicrobial peptide is such as: in the Chinese patent application with the publication number CN101456910A, a method for preparing the Rana antimicrobial peptide by leaching method is disclosed; in the Chinese patent application with the publication number CN103073628A, an ultrasonic A method for preparing Rana antimicrobial peptides by extraction method; in the Chinese patent application with publication number CN1216768A, a method for preparing Rana antimicrobial peptides by leaching method is disclosed.

The medicine for preparing and treating onychomycosis can be in various dosage forms, and the dosage forms include but are not limited to Rana antimicrobial peptide gel, Rana antimicrobial peptide spray, Rana antimicrobial peptide disinfection preparation, Rana antimicrobial peptide ointment, Rana antibacterial peptide lotion, etc.

The authorized patent whose notification number is CN1583165A discloses a Rana antimicrobial peptide gel and a preparation method thereof.

The Chinese patent application whose publication number is CN1583166A discloses a Rana antimicrobial peptide spray and a preparation method thereof.

A Chinese patent application with publication number CN101491245A discloses a disinfectant preparation containing Rana antibacterial peptide and a preparation method thereof.

The Chinese authorized patent with the publication number CN101496773A discloses a Rana antibacterial peptide lotion and a preparation method thereof.

The Chinese patent application with the publication number CN104173382A discloses a wood frog extract mixed liquid ointment and a preparation method thereof.

The present invention finds that any dosage form containing the Rana antibacterial peptide can be used for treating onychomycosis. Onychomycosis is treated with the preparation made of Rana antimicrobial peptide, the method of use is simple, the effective rate and cure rate are high, the applicable population is wide, there is no side effect, and the recurrence rate is low.

detailed description

The present invention will be further described below in conjunction with embodiment.

Example 1

According to the method described in Example 1 in the CN1583165A patent, the Rana antimicrobial peptide gel was prepared, and its pharmacodynamics was studied.

The present invention carries out clinical observation and treatment to 200 patients, including 118 males and 82 females aged 21 to 70 years old, all of whom are onychomycosis.

(1) Treatment method:

For external use, apply the Rana antimicrobial peptide gel to the affected area 4 times a day, cover the affected area evenly, and use about 1-2ml each time for each affected area, and apply it continuously for 60 days.

(2) Evaluation criteria for curative effect:

Cure: Discoloration, sunken or warped deck, rough and uneven clinical symptoms and signs disappear, and functional activities return to normal.

Effective: the clinical symptoms were significantly improved or basically disappeared.

Ineffective: no improvement in clinical symptoms and signs.

(3) Treatment effect:

168 cases were clinically cured, accounting for 84%; 34 cases were effective, accounting for 17%; 0 cases were ineffective; the total effective rate was 100%.

No recurrence was found 30 days after drug withdrawal.

Example 2

According to the method described in Example 1 of the patent application whose publication number is CN1583166A, the Rana antimicrobial peptide spray was prepared, and its pharmacodynamic study was carried out.

The present invention carries out clinical observation and treatment to 100 patients, including 63 males and 37 females, aged from 21 to 70 years old, all of whom are onychomycosis.

(1) Treatment method:

For external use, the Rana antimicrobial peptide spray is sprayed on the affected part 4 times every day, and the affected part will be evenly covered when spraying. Each dosage of each affected part is about 1-2ml, and it is used continuously for 60 days.

(2) Evaluation criteria for curative effect:

Cure: Discoloration, sunken or warped deck, rough and uneven clinical symptoms and signs disappear, and functional activities return to normal.

Effective: the clinical symptoms were significantly improved or basically disappeared.

Ineffective: no improvement in clinical symptoms and signs.

(3) Treatment effect:

79 cases were clinically cured, accounting for 79%; 21 cases were effective, accounting for 21%; 0 cases were ineffective; the total effective rate was 100%.

No recurrence was found 30 days after drug withdrawal.

Example 3

According to the method described in Example 1 in the CN101491245A patent, a disinfection preparation containing Rana antimicrobial peptide was prepared, and its pharmacodynamics was studied.

The present invention carries out clinical observation and treatment to 70 patients, including 39 males and 31 females, aged 25 to 60, all of whom are onychomycosis.

(1) Treatment method:

For external use, the affected part is soaked in the described Rana antibacterial peptide disinfection preparation 4 times every day, soaked for 5 minutes each time, and used continuously for 60 days.

(2) Evaluation criteria for curative effect:

Cure: Discoloration, sunken or warped deck, rough and uneven clinical symptoms and signs disappear, and functional activities return to normal.

Effective: the clinical symptoms were significantly improved or basically disappeared.

Ineffective: no improvement in clinical symptoms and signs.

(3) Treatment effect:

61 cases were clinically cured, accounting for 87%; 9 cases were effective, accounting for 13%; 0 cases were ineffective; the total effective rate was 100%.

No recurrence was found 30 days after drug withdrawal.

Example 4

According to the method described in Example 1 in the CN101496773A patent, the Rana antibacterial peptide lotion was prepared, and its pharmacodynamics was studied.

The present invention carries out clinical observation and treatment to 70 patients, wherein 32 cases are males, 38 cases are females, aged 25 to 55 years old, all of them are onychomycosis.

(1) Treatment method:

For external use, the affected part is soaked in the Rana antimicrobial peptide lotion 4 times every day, soaked for 5 minutes each time, and used continuously for 60 days.

(2) Evaluation criteria for curative effect:

Cure: Discoloration, sunken or warped deck, rough and uneven clinical symptoms and signs disappear, and functional activities return to normal.

Effective: the clinical symptoms were significantly improved or basically disappeared.

Ineffective: no improvement in clinical symptoms and signs.

(3) Treatment effect:

59 cases were clinically cured, accounting for 84%; 11 cases were effective, accounting for 16%; 0 cases were ineffective; the total effective rate was 100%.

No recurrence was found 30 days after drug withdrawal.

Example 5

According to the method described in Example 2 of the CN104173382A patent application, the Rana antimicrobial peptide ointment was prepared, and its pharmacodynamics was studied.

The present invention carries out clinical observation and treatment to 64 patients, wherein 35 cases are males, 29 cases are females, aged 25 to 70 years old, all of them are onychomycosis.

(1) Treatment method:

For external use, apply the Rana antibacterial peptide ointment to the affected part 4 times every day, and cover the affected part evenly when smearing. The dosage of each affected part is about 1-2ml, and it is used continuously for 60 days.

(2) Evaluation criteria for curative effect:

Cure: Discoloration, sunken or warped deck, rough and uneven clinical symptoms and signs disappear, and functional activities return to normal.

Effective: the clinical symptoms were significantly improved or basically disappeared.

Ineffective: no improvement in clinical symptoms and signs.

(3) Treatment effect:

55 cases were clinically cured, accounting for 86%; 9 cases were effective, accounting for 14%; 0 cases were ineffective; the total effective rate was 100%.

No recurrence was found 30 days after drug withdrawal.

Claims (2)

1. The use of Rana antibacterial peptide in the preparation of medicine for the treatment of onychomycosis. 2. purposes according to claim 1, it is characterized in that: the medicine of described treatment onychomycosis is Rana antimicrobial peptide gel, Rana antimicrobial peptide spray, Rana antimicrobial peptide disinfection preparation, Rana antimicrobial peptide ointment agent or Rana antimicrobial peptide lotion.