[go: up one dir, main page]
More Web Proxy on the site http://driver.im/

CN105748409A - Protopanoxadiol submicroemulsion preparation as well as preparation method and application thereof - Google Patents

Protopanoxadiol submicroemulsion preparation as well as preparation method and application thereof Download PDF

Info

Publication number
CN105748409A
CN105748409A CN201610115501.3A CN201610115501A CN105748409A CN 105748409 A CN105748409 A CN 105748409A CN 201610115501 A CN201610115501 A CN 201610115501A CN 105748409 A CN105748409 A CN 105748409A
Authority
CN
China
Prior art keywords
preparation
oil
protopanoxadiol
emulsifying agent
submicron emulsion
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201610115501.3A
Other languages
Chinese (zh)
Inventor
赵树民
廖永红
杨飞飞
周晶
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Xu Qingtong
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to CN201610115501.3A priority Critical patent/CN105748409A/en
Publication of CN105748409A publication Critical patent/CN105748409A/en
Pending legal-status Critical Current

Links

Classifications

    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00Medicinal preparations containing organic active ingredients
    • A61K31/56Compounds containing cyclopenta[a]hydrophenanthrene ring systems; Derivatives thereof, e.g. steroids
    • A61K31/575Compounds containing cyclopenta[a]hydrophenanthrene ring systems; Derivatives thereof, e.g. steroids substituted in position 17 beta by a chain of three or more carbon atoms, e.g. cholane, cholestane, ergosterol, sitosterol
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/44Oils, fats or waxes according to two or more groups of A61K47/02-A61K47/42; Natural or modified natural oils, fats or waxes, e.g. castor oil, polyethoxylated castor oil, montan wax, lignite, shellac, rosin, beeswax or lanolin
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00Medicinal preparations characterised by special physical form
    • A61K9/10Dispersions; Emulsions
    • A61K9/107Emulsions ; Emulsion preconcentrates; Micelles
    • A61K9/1075Microemulsions or submicron emulsions; Preconcentrates or solids thereof; Micelles, e.g. made of phospholipids or block copolymers

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Epidemiology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Dispersion Chemistry (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Medicinal Preparation (AREA)

Abstract

The invention provides a protopanoxadiol submicroemulsion preparation and a preparation method thereof. The protopanoxadiol submicroemulsion preparation comprises protopanoxadiol, oil, an emulsifier and a co-emulsifier, average particle size of the protopanoxadiol submicroemulsion preparation is 110-130nm, and Zeta potential ranges from -40mV to -20mV. The invention also provides an application of the protopanoxadiol submicroemulsion preparation in preparation of a medicine with the effects of promoting hematopoietic cell proliferation and alleviating bone marrow inhibiting effect caused by tumour treatment.

Description

Protopanoxadiol Submicron Emulsion preparation and its production and use
Technical field
The present invention relates to a kind of protopanoxadiol Submicron Emulsion preparation and its production and use, belong to pharmaceutical preparation neck Territory.
Background technology
Protopanoxadiol (PPD) is diol type ginsengenin, is mainly derived from the Araliaceaes such as ginseng, American Ginseng, pseudo-ginseng Plant.It is the aglycon of glycol group ginsenoside, is also glycol group ginsenoside end product after microorganism glycolysis.Research table The sodium channel that bright PPD has suppression kinds of tumors growth, antidepression, activation chloride channel, suppression to be activated depolarizes, suppression people Many activity such as the growth of embryonic kidney cell HEK-293 cell and growth of H. pylori.Meanwhile, cerebral damage is had by PPD Protective effect.
PPD is lipophilic compound, and in water, dissolubility is poor, and oral administration biaavailability is low, and the half-life is short.Need protoplast The preparation of ginseng glycol carries out studying to improve bioavilability.The researchs such as hair Jingjing are prepared for PPD dry suspensoid agent, and this formulation is improved Drug absorption, and do not affected by food, particle is big at intestines and stomach distribution areas, absorbs fast, and bioavilability is carried Height (sees a mao Jingjing, Zhang Tong, Wang Bing etc..The prescription of 20 (S)-protopanoxadiol dry suspensoid agents and preliminary quality research.Middle one-tenth Medicine, 2013,34 (9): 1680-1684).Jin Shengxuan etc. are prepared for PPD liposome for intravenous injection, pass to realizing targeting Medicine, plays antitumor action and (sees Jin Shengxuan, Lei Rongjian, Sun Jingyun.HPLC measures containing of 20 (s)-Protopanaxadiol liposome Amount and envelop rate.Nanjing University of Traditional Chinese Medicine's journal, 2009,25 (3): 197-198).Jin Xin etc. are prepared for the protoplast of PPD and join lipid Cubic liquid crystal nanoparticle, lipid cubic liquid crystal by polar lipid in aqueous environment by adsorb quantitative water formed have special The gellike of internal structure cube skeleton structure mutually, has stronger adhesiveness, thus promotes that medicine cross-film absorbs.Preparation is set up After side's liquid crystal, adding the medicine holdup time in vivo, Relative oral bioavailability is 1.66 times of bulk drug and (sees gold Prosperous, Zhang Zhenhai, Sun E etc., protopanoxadiol lipid-based cubic liquid crystalline nanoparticle pharmacokinetic studies in rat body.Chinese medicine Magazine, 2013,38 (2): 263-268).Drug carrier passed by emulsion can increase solubility and the stability of insoluble drug, oil Phase and emulsifying agent can degrade and absorb in vivo, and stable in properties does not produce superman's body standard osmotic pressure after intravenous.Warp of the present invention Patent consulting and literature search, the most not yet find PPD is prepared as emulsion for injection, carry out the report of antitumor research.
Summary of the invention
It is an object of the present invention to provide a kind of protopanoxadiol Submicron Emulsion preparation;
Second object of the present invention is to provide the preparation method of this protopanoxadiol Submicron Emulsion preparation;
Third object of the present invention is to provide the Submicron Emulsion preparation prepared by protopanoxadiol and the present invention in preparation There is the application in the medicine promoting hematopoietic cell proliferation, alleviation bone marrow inhibition.
A kind of protopanoxadiol Submicron Emulsion preparation, including protopanoxadiol, oil, emulsifying agent, assistant for emulsifying agent, its average grain diameter Being 110~130nm, Zeta potential is-20~-40mV.This protopanoxadiol Submicron Emulsion preparation is through high pressure, centrifugal, dilution process The most moderately good, in 4,20,30 DEG C of stability tests 4 months, have good stability.
In some embodiments, described protopanoxadiol content is 0.1-0.5%, oil phase content 10-30%, emulsifying agent With assistant for emulsifying agent content 3-4%;Preferably, emulsifying agent is 2:1~2 with the ratio of assistant for emulsifying agent;It is further preferred that oil phase contains Amount is 10%, and emulsifying agent is 2:1.5 with the ratio of assistant for emulsifying agent.
In some embodiments, described grease separation is from vegetable oil, animal oil, mineral oil or artificial oil;Described plant grease separation Any one or a few in soybean oil, corn oil, peanut oil, olive oil, castor oil, palm oil;Described animal oil is selected from Fish oil or oleic acid;Described mineral oil is selected from any one or a few in aliphatic hydrocarbon, cerul hydrocarbon, aromatic hydrocarbon or paraffin oil;Described Artificial oil is selected from linoleic acid, ethyl oleate, rilanit special, hydrogenated groundnut, cotmar, ethyl linoleate, self-emulsifying Any one or a few in monostearate, pungent/glycerol decanoate, isopropyl myristate or triethylglycerides;Further Preferably, described oil is soybean oil or peanut oil.
In some embodiments, described emulsifying agent is selected from Fabaceous Lecithin, yolk phospholipid, phosphatidyl-ethanolamine, serine phosphorus Any one or a few in fat, lipositol, phosphatidic acid;The most described emulsifying agent is egg yolk lecithin or soybean ovum phosphorus Fat.
In some embodiments, described assistant for emulsifying agent is selected from poloxamer (F68) or solutol.
The preparation method that present invention also offers described protopanoxadiol Submicron Emulsion preparation comprises the steps:
Step 1, the preparation of oil phase: add emulsifying agent and effective dose PPD in appropriate oil, dispersion obtains oil phase;
Step 2, the preparation of aqueous phase: qs glycerin, assistant for emulsifying agent are dissolved in suitable quantity of water, make aqueous phase;
Step 3, just emulsification: under the conditions of 60~70 DEG C, oil phase is added aqueous phase, make colostrum;
Step 4, homogeneous: taking the primary emulsion of step 3 preparation, homogeneous, emulsification, is 110~130nm to emulsion droplet average grain diameter;
Step 5, filling, sterilizing.
In some embodiments, step 3 carries out ultrasonically treated after oil phase adds aqueous phase, and ultrasonic time is 5-60min; Step 4 homogeneous is carried out in high pressure homogenizer, and homogenization pressure is 500-2000bar, and cycle-index is 5-20 time.Preferably, super The sound time is 10-20min;Step 4 homogenization pressure is 1000-2000bar, and cycle-index is 5-15 time.It is further preferred that it is super The sound time is 10min;Homogenization pressure is 2000bar, and cycle of higher pressure number of times is 10 times.
The present invention also provides for protopanoxadiol and has the bone promoting hematopoietic cell proliferation, alleviating oncotherapy generation in preparation Application in the medicine of marrow inhibitory action.
Internal experiment in vitro all prove protopanoxadiol emulsion for injection can be obviously promoted bone marrow cell grain system (CFU-GM), Red system (CFU-E/BFU-E) hematopoietic progenitor population generates.Show that protopanoxadiol emulsion for injection has promotion hematopoietic cell The effect of the bone marrow suppression that propagation, alleviation oncotherapy produce.
Accompanying drawing explanation
Fig. 1 PPD Submicron Emulsion formulation aesthetics form
Form under Fig. 2 PPD Submicron Emulsion preparation transmission electron microscope
Specific embodiment mode
Embodiment 1
Prescription:
Embodiment 2
Embodiment 3
Prescription:
Embodiment 4
Prescription:
Embodiment 5
Prescription:
Embodiment 6
The preparation method of the arbitrary prescription of embodiment 1~5: oil phase (soybean oil or peanut oil) is stirred in 80 DEG C and adds breast Agent, high speed dispersion, to becoming settled solution, then adds protopanoxadiol at 60 DEG C, and high speed dispersion uniformly obtains oil phase;Separately Adding 20ml glycerine and assistant for emulsifying agent in 800ml water, 60 DEG C are uniformly mixing to obtain aqueous phase.Under 60 DEG C of high-speed stirred, by oil Slowly it is added drop-wise to aqueous phase mutually, continues ultrasonic agitation 10 minutes, be settled to 1000ml.By colostrum through high-pressure homogeneous 2000bar pressure Circulate 10 times, filling, sterilizing, both.
The Submicron Emulsion preparation physical parameter that embodiment 1~5 prescription is prepared by the present embodiment method
Embodiment 7
The preparation method of the arbitrary prescription of embodiment 1~5, substantially the same manner as Example 6, difference is: the ultrasonic agitation time It it is 15 minutes.
Its average grain diameter of Submicron Emulsion preparation that the present embodiment method prepares is 115~125nm, and Zeta potential is-30 ~-40mV.
Embodiment 8
The preparation method of the arbitrary prescription of embodiment 1~5, substantially the same manner as Example 6, difference is: the ultrasonic agitation time It it is 20 minutes.
Its average grain diameter of Submicron Emulsion preparation that the present embodiment method prepares is 115~125nm, and Zeta potential is-30 ~-40mV.
Embodiment 9
The preparation method of the arbitrary prescription of embodiment 1~5, substantially the same manner as Example 6, difference is: high-pressure homogeneous pressure For 1000bar, high-pressure homogeneous cycle-index is 10.
Its average grain diameter of Submicron Emulsion preparation that the present embodiment method prepares is 115~125nm, and Zeta potential is-30 ~-40mV.
Embodiment 10
The preparation method of the arbitrary prescription of embodiment 1~5, substantially the same manner as Example 6, difference is: high-pressure homogeneous pressure For 700bar, high-pressure homogeneous cycle-index is 15.
Its average grain diameter of Submicron Emulsion preparation that the present embodiment method prepares is 115~125nm, and Zeta potential is-30 ~-40mV.
Embodiment 11
The preparation method of the arbitrary prescription of embodiment 1~5, substantially the same manner as Example 6, difference is: first emulsifying temperature is 70℃。
Its average grain diameter of Submicron Emulsion preparation that the present embodiment method prepares is 115~125nm, and Zeta potential is-30 ~-40mV.
Embodiment 12
The preparation method of the arbitrary prescription of embodiment 1~5, substantially the same manner as Example 6, difference is: first emulsifying temperature is 40℃。
Its average grain diameter of Submicron Emulsion preparation that the present embodiment method prepares is 230~240nm.
Embodiment 13
The preparation method of the arbitrary prescription of embodiment 1~5, substantially the same manner as Example 6, difference is: first emulsifying temperature is 80℃。
Its average grain diameter of Submicron Emulsion preparation that the present embodiment method prepares is 240~250nm.
Submicron Emulsion preparation stability is investigated:
Test Submicron Emulsion preparation is the Submicron Emulsion preparation that embodiment 1 prescription is prepared by embodiment 6 preparation method.
The sign of Submicron Emulsion:
Proterties: outward appearance presents dispersed, milky Submicron Emulsion preparation, weak blue fluorescence (see Fig. 1) seen from bottle wall.
PH value: PPD Submicron Emulsion between pH value 8.2-8.4, has prepared unpasteurized PPD Submicron Emulsion pH when being prepared as colostrum Value is between 7.9-8.0, and the PPD Submicron Emulsion pH value after sterilizing is between 7.0-7.2.
Submicron Emulsion morphologic observation: take the appropriate distilled water diluting of Submicron Emulsion to debita spissitudo, dripped by micro emulsion on copper mesh, uses 2% phosphotungstic acid negative staining 1min, with observing the form of emulsion droplet, for black even scattered spherical (see Fig. 2) under transmission electron microscope.
Stability:
1, the autoclaving impact on Submicron Emulsion
Take with a collection of Submicron Emulsion, be divided into 2 parts, and a pressure sterilizing (105 DEG C, 45min;115 DEG C, 30min and 121 DEG C, 15min), a without pressure sterilizing, observe the outward appearance of 2 parts of Submicron Emulsions, compare the change of particle diameter, current potential and medicament contg simultaneously Change.The results are shown in Table 1.
The impact on PPD Submicron Emulsion of table 1 autoclaving
Result shows PPD Submicron Emulsion stable in physicochemical property after autoclaving.
2, dewatering ability
By Submicron Emulsion three batches, 3500rmp, centrifugal 20min, seen whether that medicine crystal grain separates out, mensuration particle diameter, current potential, Medicament contg.The results are shown in Table 2.
The dewatering ability of table 2 PPD Submicron Emulsion
The dewatering ability of result display PPD Submicron Emulsion is good.
3, dilution stability
By Submicron Emulsion three batches, after diluting 10,50,100,200,500,1000,2000 times respectively, see whether that medicine is brilliant Size separation goes out, and measures particle diameter, current potential.The results are shown in Table 3.
The dilution stability of table 3 PPD Submicron Emulsion
Multiple Particle diameter (nm) PDI Current potential (-mV)
0 119.7±0.9 0.142±0.022 -35.6±1.6
10 132.9±0.8 0.198±0.019 -33.5±0.3
50 120.1±1.3 0.145±0.021 -33.4±1.0
100 119.7±0.9 0.142±0.022 -35.6±1.6
200 120.6±1.0 0.143±0.009 -38.8±3.9
500 118.5±0.7 0.149±0.024 -37.6±0.6
1000 120.7±0.3 0.154±0.009 -28.1±0.8
2000 124.4±0.2 0.203±0.026 -23.4±3.7
Result display dilution is stable, dilutes 2000 times of change of size still less than 5%.
4, PPD Submicron Emulsion places the stability of 1,2,3,4,5 months respectively
Table 41 month shelf-stability of PPD Submicron Emulsion
Table 52 months shelf-stabilities of PPD Submicron Emulsion
Table 63 months shelf-stabilities of PPD Submicron Emulsion
Table 74 months shelf-stabilities of PPD Submicron Emulsion
PPD Submicron Emulsion preparation prepared by the present invention, respectively at 4,20,30 DEG C of stability tests 4 months, has good stability.
The impact on chemotherapy mouse hematopoetic cell of the Submicron Emulsion preparation
1, the external dosing of protopanoxadiol emulsion for injection is to bone marrow suppression bone marrow cells in mice grain system (CFU-GM), red system (CFU-E/BFU-E) impact that hematopoietic progenitor population generates.
After experiment uses endoxan 200mg/kg, modeling of lumbar injection, modeling three days, extracting marrow cell collects Fall to cultivating, in cultivating system, add the medicine of variable concentrations simultaneously.The results are shown in Table 8.
Protopanoxadiol emulsion for injection can be obviously promoted three kinds of HPCs increasings in vitro as can be seen from the results Growing, colony productivity is obviously higher than model group (P < 0.05 or 0.01).
The impact on bone marrow suppression mouse hemopoietic progenitor population number of the table 8 protopanoxadiol emulsion for injection
Compare with model comparison*P < 0.05,**P < 0.01,***P<0.001
2, in protopanoxadiol emulsion for injection body, dosing is thin to bone marrow suppression bone marrow cells in mice grain system, erythroid hematopoiesis ancestral The impact of born of the same parents' colony formation.
Experimental day intraperitoneal injection of cyclophosphamide (CP) modeling, after modeling, the intravenous injection immediately of administration group gives protoplast and joins two Alcohol emulsion for injection medicine, Normal group and model group give the distilled water of same volume, be administered volume and be 0.2ml/10g, Successive administration 7 days, 7d often group takes 3 and carries out HPC cultivation the most upon administration, within the 1st, 3,5,7 day after modeling, takes Blood surveys routine blood test.
1. Colony cultivation result: result can be seen that the protopanoxadiol emulsion for injection of various dose is little from following table Three kinds of proliferation of hematopoietic progenitors can be obviously promoted in mouse body;
The impact on three kinds of proliferation of hematopoietic progenitors of mouse of the table 9 protopanoxadiol emulsion for injection
* P < 0.05, * * P < 0.01, * * * P < 0.001 is compared with model comparison
2. routine blood test result: from following table, the 7th day 75 and 150mg/kg dosage group murine interleukins after administration (WBC) raise clearly.
Table 10 routine blood test measurement result
Basis 3rd day 5th day 7th day
Normal group 4.43±1.75 6.92±0.59 7.10±0.76 8.05±1.44
Model group 4.43±1.73 2.17±0.33 1.90±0.37 5.17±0.37
75mg/kg group 4.57±2.19 1.87±0.47 1.89±0.81 19.24±5.16*
150mg/kg group 4.60±2.30 2.17±0.34 1.71±0.37 21.86±2.25***
* P < 0.05, * * P < 0.01, * * * P < 0.001 is compared with model comparison.

Claims (10)

1. a protopanoxadiol Submicron Emulsion preparation, it is characterised in that described Submicron Emulsion preparation includes protopanoxadiol, oil, breast Agent, assistant for emulsifying agent, its average grain diameter is 110~130nm, and Zeta potential is-20~-40mV.
2. preparation as claimed in claim 1, it is characterised in that in described Submicron Emulsion preparation, each component content is: protoplast joins two Alcohol 0.1-0.5%, oil 10-30%, emulsifying agent and assistant for emulsifying agent 3-4%.
3. preparation as claimed in claim 2, it is characterised in that described oil content is 10~15%, emulsifying agent and assistant for emulsifying agent Ratio be 2:1~2.
4. preparation as claimed in claim 1, it is characterised in that described grease separation is from vegetable oil, animal oil, mineral oil or synthesis Oil;Described emulsifying agent is in Fabaceous Lecithin, yolk phospholipid, phosphatidyl-ethanolamine, serinephosphatide, lipositol, phosphatidic acid Any one or a few;Described assistant for emulsifying agent is selected from poloxamer or solutol.
5. preparation as claimed in claim 1, it is characterised in that described vegetable oil is soybean oil or peanut oil;Described emulsifying agent For Fabaceous Lecithin or yolk phospholipid.
6. the preparation method of protopanoxadiol Submicron Emulsion preparation as described in any one of claim 1-5, it is characterised in that described side Method comprises the steps:
Step 1, the preparation of oil phase: add emulsifying agent and effective dose protopanoxadiol in appropriate oil, dispersion obtains oil phase;
Step 2, the preparation of aqueous phase: qs glycerin, assistant for emulsifying agent are dissolved in suitable quantity of water, make aqueous phase;
Step 3, just emulsification: under the conditions of 60~70 DEG C, oil phase is added aqueous phase, make colostrum;
Step 4, homogeneous: taking the primary emulsion of step 3 preparation, homogeneous, emulsification, is 110~130nm to emulsion droplet average grain diameter;
Step 5, filling, sterilizing.
7. method as claimed in claim 6, it is characterised in that step 3 carries out ultrasonically treated after oil phase adds aqueous phase, ultrasonic Time is 5-60min;Step 4 homogenization pressure is 500-2000bar, and cycle-index is 5-20 time.
8. method as claimed in claim 7, it is characterised in that ultrasonic time is 10-20min;Homogenization pressure is 1000- 2000bar, cycle-index is 5-15 time;Preferably, ultrasonic time is 10min;Homogenization pressure is 2000bar, cycle of higher pressure time Number is 10 times.
9. the protopanoxadiol Submicron Emulsion preparation that as claimed in claim 6 prepared by method promotes hematopoietic cell proliferation or slow in preparation Solve the application in the medicine of the bone marrow inhibition that oncotherapy produces;Preferably facilitate bone marrow cell grain system and/or erythroid hematopoiesis Progenitor population generates.
10. protopanoxadiol promotes hematopoietic cell proliferation in preparation or alleviates the medicine of the bone marrow inhibition that oncotherapy produces In application;Preferably facilitate bone marrow cell grain system and/or hemopoietic progenitor cell of red blood cell line colony formation.
CN201610115501.3A 2016-03-01 2016-03-01 Protopanoxadiol submicroemulsion preparation as well as preparation method and application thereof Pending CN105748409A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201610115501.3A CN105748409A (en) 2016-03-01 2016-03-01 Protopanoxadiol submicroemulsion preparation as well as preparation method and application thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201610115501.3A CN105748409A (en) 2016-03-01 2016-03-01 Protopanoxadiol submicroemulsion preparation as well as preparation method and application thereof

Publications (1)

Publication Number Publication Date
CN105748409A true CN105748409A (en) 2016-07-13

Family

ID=56332203

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201610115501.3A Pending CN105748409A (en) 2016-03-01 2016-03-01 Protopanoxadiol submicroemulsion preparation as well as preparation method and application thereof

Country Status (1)

Country Link
CN (1) CN105748409A (en)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110623926A (en) * 2019-10-31 2019-12-31 沈阳药科大学 Panaxatriol supersaturated self-microemulsion and preparation method thereof
CN112263585A (en) * 2020-11-04 2021-01-26 复旦大学附属妇产科医院 Application of protopanoxadiol PPD in preparing medicine for treating infertility and abortion
CN114425038A (en) * 2022-01-27 2022-05-03 沈阳信康药物研究有限公司 20(S) -PPD liposome emulsion complex oral administration preparation and preparation method and application thereof
CN114831935A (en) * 2022-05-18 2022-08-02 沈阳信康药物研究有限公司 Sterile 20(S) -PPD oral liquid preparation capable of being administrated by nasal feeding and preparation method and application thereof

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110623926A (en) * 2019-10-31 2019-12-31 沈阳药科大学 Panaxatriol supersaturated self-microemulsion and preparation method thereof
CN110623926B (en) * 2019-10-31 2022-05-20 沈阳药科大学 Panaxatriol supersaturated self-microemulsion and preparation method thereof
CN112263585A (en) * 2020-11-04 2021-01-26 复旦大学附属妇产科医院 Application of protopanoxadiol PPD in preparing medicine for treating infertility and abortion
CN114425038A (en) * 2022-01-27 2022-05-03 沈阳信康药物研究有限公司 20(S) -PPD liposome emulsion complex oral administration preparation and preparation method and application thereof
CN114425038B (en) * 2022-01-27 2023-03-10 沈阳信康药物研究有限公司 20 (S) -PPD liposome emulsion complex oral administration preparation and preparation method and application thereof
CN114831935A (en) * 2022-05-18 2022-08-02 沈阳信康药物研究有限公司 Sterile 20(S) -PPD oral liquid preparation capable of being administrated by nasal feeding and preparation method and application thereof
CN114831935B (en) * 2022-05-18 2023-04-07 沈阳信康药物研究有限公司 Sterile 20 (S) -PPD oral liquid preparation capable of being administrated by nasal feeding and preparation method and application thereof

Similar Documents

Publication Publication Date Title
CA2194226C (en) Stable oil-in-water emulsions incorporating a taxine (taxol) and method of making same
CN100569294C (en) A kind of used for intravenous injection high stable long-circulation fat fat breast carrying medicine
CN104427977B (en) Depot formulations of local anesthetic and preparation method thereof
CN101396343B (en) Paclitaxel submicron emulsion using lipid composite as middle carrier
CN106456541A (en) Compositions of nanoemulsion delivery systems
CN105748409A (en) Protopanoxadiol submicroemulsion preparation as well as preparation method and application thereof
CN102686217B (en) Submicro emulsion of paclitaxel using steroid complex as intermediate carrier
CN101909614A (en) Nanodispersion
CN101244053B (en) Novel dispersed system with docetaxel as main component
CN100496609C (en) Stable liposome composition
CN101829052B (en) Self-emulsifying preparation of taxane compound and preparation method thereof
CN108159055A (en) Treat long-acting delivery system, preparation method and the application of breast cancer
CN114796110A (en) Insoluble drug concentrated solution without ethanol and micelle solution prepared from insoluble drug concentrated solution
CN102552137B (en) Triptolide fat emulsion injection and preparation method thereof
CN103405385A (en) Temozolomide intravenous injection fat emulsion and preparation method thereof
JPH10510267A (en) Emulsion suitable for administration of sphingolipid and use thereof
CN100375621C (en) Vinorelbine liposome micro ball injection and its prepn
DK173596B1 (en) Emulsion for parenteral administration
CN101322688B (en) Flumazenil oil-in-water emulsion for vein and preparation thereof
CN100515389C (en) Medicinal emulsion adapted for difficultly soluble medicine and method for preparing the same
CN105832744B (en) A kind of Alprostadil freeze-dried emulsion composition of injection
TW201642834A (en) Injecting emulsion of protopanaxadiol and preparation method thereof
CN113456592A (en) Antiseptic and anti-inflammatory liposome and preparation method thereof
CN100569226C (en) A kind of long-cycled lipid emulsion profofol preparation
CN102228431A (en) Self-emulsified medicinal composition for taxane compounds

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C41 Transfer of patent application or patent right or utility model
TA01 Transfer of patent application right

Effective date of registration: 20170105

Address after: 100044 Beijing city Haidian District Shangyuan Village Hospital No. 3 east 5 Building No. 142

Applicant after: Xu Qingtong

Address before: 100085 Zhongguancun biological medicine Park, Haidian District, Beijing, No. 5 on the road to open up B-201

Applicant before: Zhao Shumin

WD01 Invention patent application deemed withdrawn after publication
WD01 Invention patent application deemed withdrawn after publication

Application publication date: 20160713