CN105532398A - Light-matrix degradable-nonwoven-fabric ex-vitro-rooting seedling raising method for Hosta plantaginea tissue culture seedlings - Google Patents
Light-matrix degradable-nonwoven-fabric ex-vitro-rooting seedling raising method for Hosta plantaginea tissue culture seedlings Download PDFInfo
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- CN105532398A CN105532398A CN201510957387.4A CN201510957387A CN105532398A CN 105532398 A CN105532398 A CN 105532398A CN 201510957387 A CN201510957387 A CN 201510957387A CN 105532398 A CN105532398 A CN 105532398A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
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Abstract
The invention discloses a light-matrix degradable-nonwoven-fabric ex-vitro-rooting seedling raising method for Hosta plantaginea tissue culture seedlings. The method specifically comprises the following steps of carrying out matrix preparation, carrying out transition seedling hardening, carrying out seedling selecting and plant division, carrying out rooting agent preparing and rooting agent treatment, carrying out tissue culture seedling transplanting and carrying out transplanted management. According to the method, the production cost can be reduced by 27%, and the tissue culture space is saved by 35%, so that the method is of great practical significance in ecological environmental protection and the industrialized production of excellent Hosta plantaginea varieties.
Description
Technical field
The invention belongs to field of plant tissue culture technique, be specifically related to a kind of jade hairpin plantlet in vitro Light media degradable nonwoven fabrics outside sprout-cultivating-bottle seedling method.
Background technology
Hosta (Hosta) plant is Liliaceae (Liliaceae) perennial root draft, originates in east, and wild species with Japan's distribution at most, is secondly China.Because it is wide in variety, form shows unique characteristics, and viewing period is long, strong adaptability, can be used as desirable sight leaf and sees flower ground quilt, underground, potted plantly to view and admire and cut-flower.In addition, containing the compositions such as abundant polysaccharide, saponin(e, trace element due to it, itself possess higher medical value, is one of the medicinal plant having DEVELOPMENT PROSPECT in future.Become at present one of herbage flower that sales volume in the world comes out at the top, widely cultivated all over the world.
Blue plantain-lily is that China originates in one of 4 large Hosta cultivars, and leafage is raw, and glossy, the florescence is time autumn in summer, and petal is like hairpin, and bluish violet, smell delicate fragrance, the Hosta ventricosa that is otherwise known as, beautiful Chinese bush cherry flower, have higher ornamental value.Floral leaf jade hairpin is external introduced variety, and leaf look distinct, blade face secondary color, the vein that visible train of thought is distinct, is also potted plant, greening kind that proterties is excellent.
Traditional jade hairpin propagation method is sowing and division propagation, and seed propagation needs 3 ~ 5a to bloom, and there is qualitative variation, degradation phenomena; Division propagation all can carry out in Growing season, but has the inferior position that reproduction coefficient is low, the cycle is long.Relative to Traditional breeding processes, tissue cultures can expand numerous improved seeds in a short time fast, in a large number, and the factorial praluction for jade hairpin has huge realistic meaning.In recent years, report for the group culturation rapid propagating technology of jade hairpin increases gradually, technology reaches its maturity, and mainly round aspects such as searching tissue cultures optimum condition, reduction production cost and shortening production cycles, substantially adopts the traditional approach of taking root in bottle in taking root.Take root, important step that hardening is traditional group training, simultaneously the new outside sprout-cultivating-bottle technology risen to carry out taking root with domestication in recent years, saving group training space while reduction production cost.Outside sprout-cultivating-bottle technology is at masson pine (patent No. CN103461121A), photinia glabra (patent No. CN103548685A), blueberry (patent No. CN103704109A), honeysuckle (patent No. CN103548653A) sugarcane (patent No. CN101904262A), the plants such as cuckoo (patent No. CN103371093A) all there is patent report, jade hairpin only there is one section, above method all selects traditional container for plant growth (seedling-raising box), generic media (turfy soil, loess, perlite, , river sand etc.) outside sprout-cultivating-bottle is carried out to plantlet in vitro, after taking root, container for plant growth not only k-index growth, there is nest root phenomenon, and can need reuse after reclaiming, matrix quality weight, be unfavorable for transfer transport.Disclose a kind of forest non-woven fabrics nursery light ground mass in patent publication No. CN102173929A, matrix comprises the material of main part and fertilizer, pasteurization material and water-loss reducer that are made up of peat, perlite, vermiculite, papermaking straw pulp waste material.But the material of main part peat in its matrix goes through the atmospheric swamp ground product formed for thousand, nutritious, but peat belongs to non-renewable resources, and a large amount of exploitation is very large to environmental disruption; Though the use of papermaking straw pulp waste material is twice laid, its source is subject to the restriction of production site in varying degrees.
Summary of the invention
The object of this invention is to provide a kind of jade hairpin plantlet in vitro Light media degradable nonwoven fabrics outside sprout-cultivating-bottle seedling method, utilize light ground mass as rooting media, degradable nonwoven fabrics bag is as container for plant growth, while Rapid Rooting, raising quality of rooting, reduction group training space, seedling cost, shorten growing-seedling period, convenient transport, transplanting.
The technical solution adopted in the present invention is, a kind of jade hairpin plantlet in vitro Light media degradable nonwoven fabrics outside sprout-cultivating-bottle seedling method, specifically comprises the following steps:
1) matrix prepares: select perlite, vermiculite, coconut palm chaff or ash to be that Medium Culture is tolerant, Homogeneous phase mixing, is configured to matrix; Matrix is loaded in non-woven bag, neatly put in pallet, soak into matrix with 1000 times of tpns and 1000 times of carbendazim mixed liquors and carry out disinfection, again spray before formal use;
2) transition hardening: bottle seedling of being grown thickly by the jade hairpin after group training propagation, from airtight hardening 2 ~ 3d group training room is transferred to natural lighting, then to unclamp after lid hardening 1 ~ 2d uncap hardening 2d completely, allows a bottle seedling progressively adapt to external environment condition;
3) seedling plant division is selected: bottle seedling of growing thickly after choosing propagation being highly 4 ~ 7cm, carefully clean the medium of root with running water after taking out from tissue culture bottle, with operating scissors, bottle seedling is divided into individual plant, if there is the budlet just sprouting on individual plant side, budlet is retained subsidiary by highly suitable seedling of taking root;
4) root-growing agent preparation and root-growing agent process: take root-promoting hormone in proportion, slowly pours clear water constant volume after dissolving with a small amount of ethanol, and the jade hairpin seedling base portion 1 ~ 2cm after plant division immerses in solvent of taking root and takes out after 5s by preparation root-growing agent;
5) plantlet in vitro is transplanted: with bamboo let in the punching of matrix central authorities, the degree of depth is 1.5cm, and seedling is placed in hole, and finger gently pinches cloth bag, and jade hairpin seedling base portion is fully combined with matrix;
6) management after transplanting: be placed in transparent plastic Small plastic shed after rooting and transplant, spray broad-spectrum germicide, on take sunshade net, taking root first week, all cover with film and sunshade net, keep air humidity, in shed, temperature is 18 ~ 30 DEG C, high temperature is opened shed two ends and is carried out aeration-cooling 1h daytime after one week, need not open when winter temperature is low, open sunshade net after taking root gradually and increase illuminance, midway alternately sprays weekly 800 ~ 1000 times of tpns and 800 ~ 1000 times of carbendazim, and soil humidity is accomplished not do not water; After seedling is substantially all taken root, open sunshade net and film gradually, make seedling adapt to external environment condition completely gradually.
Further, step 1) in take root container for size after filling matrix be diameter 5cm, the degradable nonwoven fabrics bag of height 7cm, in soil, the degradable time is 1 year.
Further, step 1) in matrix be content be ash, perlite, vermiculite and coconut palm chaff, volume ratio is the light environment protection matrix of 1:1:1:1.
Further, step 4) in root-growing agent be concentration be 100mg/LIBA+100mg/LNAA mixed liquor, root-growing agent soak time is 5s.
Further, step 6) in the shading rate of sunshade net be 50% ~ 75%; Described air humidity is 80% ~ 90%, and temperature of taking root is 18 ~ 30 DEG C.
The invention has the beneficial effects as follows: outside sprout-cultivating-bottle seedling method of the present invention all has no report in jade hairpin nursery and other plant.The present invention improves traditional outside sprout-cultivating-bottle seedling medium and container, select water binding capacity, air capacity of soils is good, transmit the strong Light media of nutritional capacity to make a living foundation matter, root system of plant easily penetrates, degradable, do not reclaim, the Non-woven cloth bag of nest root is not container of taking root, carry out the outside sprout-cultivating-bottle of blue plantain-lily and floral leaf jade hairpin, environment friendly and pollution-free, can transplant together with sack and transport after taking root, root system can penetrate cloth bag, realize explosion type growth, in addition, the method can reduce production cost, saving group training space, shorten growing-seedling period, the industrialization production of ecological environmental protection and jade hairpin improved seeds is of great immediate significance.
The present invention, on the basis of jade hairpin tissue culture technology maturation, has carried out the research of floral leaf jade hairpin and blue plantain-lily plantlet in vitro Light media degradable nonwoven fabrics outside sprout-cultivating-bottle seedling growing process.Study different root-promoting hormone kind and concentration by experiment to rooting rate and several impact of taking root, find suitable root-promoting hormone and concentration to be: 100mg/LIBA+100mg/LNAA mixed liquor; Research is different by experiment takes root matrix to the impact of taking root, and finds optimum matrix of taking root to be the light environment protection matrix that 1:1:1:1 mixes by ash, vermiculite, perlite, coconut palm chaff according to volume ratio; By the growing state of jade hairpin plantlet in vitro root in contrast common vessel and non-woven bag, can direct vision to non-woven bag degradable, without the need to de-bag transplantings, well developed root system, less than around root and the more powerful advantage of later stage growth.The present invention is perfect further jade hairpin tissue-culturing rapid propagation industrial breeding technique; rooting rate reaches 96.3%; mean elements reaches 13.8; whole rooting process can reduce production cost 27%; saving group training space 35%, to realizing ecological environmental protection and the extensive nursery of jade hairpin batch production has important practical significance.
Accompanying drawing explanation
Fig. 1 to be taken root situation map with the jade hairpin of common vessel plantation after taking root in bottle;
Fig. 2 is that the outer non-woven fabrics of blue plantain-lily bottle of the present invention is taken root nursery figure;
Fig. 3 is that the outer non-woven fabrics of floral leaf jade hairpin bottle of the present invention is taken root nursery figure.
Embodiment
Below in conjunction with embodiment, the present invention is described in detail.
Embodiment 1
Specifically comprise the following steps:
1) matrix prepares: select perlite, vermiculite, coconut palm chaff and ash to be that Medium Culture is tolerant, Homogeneous phase mixing, be configured to No. 1 matrix and No. 2 matrix, wherein, No. 1 matrix is: volume ratio is the ash of 1:1:1, perlite and vermiculite; The ash of No. 2 matrix to be volume ratio be 1:1:1:1, perlite, vermiculite and coconut palm chaff; Two kinds of matrix being filled respectively diameter to dress matrix is 5cm, be highly in the non-woven bag of 7cm, neatly put in pallet, soak into matrix with 1000 times of tpns and 1000 times of carbendazim mixed liquors and carry out disinfection, again spray before formal use, to strengthen Disinfection Effect.
2) transition hardening: bottle seedling of being grown thickly by the blue plantain-lily after group training propagation, from airtight hardening 2 ~ 3d group training room is transferred to natural lighting, then to unclamp after lid hardening 1 ~ 2d uncap hardening 2d completely, allows a bottle seedling progressively adapt to external environment condition.
3) seedling plant division is selected: bottle seedling of growing thickly after choosing propagation being highly 4 ~ 7cm, carefully clean the medium of root with running water after taking out from tissue culture bottle, with operating scissors, bottle seedling is divided into individual plant, if there is the budlet just sprouting on individual plant side, budlet can be retained subsidiary by highly suitable seedling of taking root.
4) root-growing agent preparation and root-growing agent process: take root-promoting hormone methyl α-naphthyl acetate NAA and indolebutyric acid IBA in proportion, slowly pours clear water constant volume after dissolving with a small amount of ethanol.Compound concentration is 200mg/LNAA solution, and 100mg/LIBA+100mg/LNAA mixed liquor two kinds of root-growing agents.Blue plantain-lily seedling base portion 1 ~ 2cm after plant division is immersed in above two kinds of solvents of taking root respectively and takes out after 5s.
5) plantlet in vitro is transplanted: with bamboo let No. 1 and the punching of No. 2 matrix central authorities, the degree of depth is about 1.5cm, and seedling is placed in hole, and finger gently pinches cloth bag, and blue plantain-lily seedling base portion is fully combined with matrix.
6) management after transplanting: be placed in transparent plastic Small plastic shed after rooting and transplant, spray broad-spectrum germicide thiophanate methyl 1000 times of liquid, above take the sunshade net that shading rate is 50% ~ 75%.Taking root first week, all cover with film and sunshade net, air humidity is kept to be 80% ~ 90%, in shed, temperature is 18 ~ 30 DEG C, high temperature can be opened shed two ends and carries out aeration-cooling 1h daytime after one week, can open when winter temperature is low, can open sunshade net after taking root gradually increases illuminance.The every first quarter moon in midway alternately sprays 800 ~ 1000 times of tpns and 800 ~ 1000 times of carbendazim, and soil humidity is accomplished not do not water.After seedling is substantially all taken root, open sunshade net and film gradually, make seedling adapt to external environment condition completely gradually.Take root number and rooting rate is added up after 40d.
The impact that table 1 different disposal condition is taken root on blue plantain-lily
As shown in Table 1, the condition of the applicable blue plantain-lily outside sprout-cultivating-bottle that the present invention obtains is: blue plantain-lily subculture tufted seedling is divided into individual plant, ash is implanted by after quick for base portion 1 ~ 2cm 100mg/LIBA+100mg/LNAA dipping 5s, vermiculite, perlite and coconut palm chaff volume ratio are carry out culture of rootage in the light environment protection matrix of 1:1:1:1, rooting rate can reach 96.3%, number of on average taking root reaches 13.8. and rooting efficiency is as shown in Figure 2, can know from Fig. 1, after utilizing common vessel and matrix to plant a period of time after taking root in jade hairpin bottle there is obvious nest root phenomenon in root system, can obtain from Fig. 2, the distance rooting treatment time is 40d, obviously can see that part blue plantain-lily root system penetrates cloth bag.
Embodiment 2
Floral leaf jade hairpin plantlet in vitro Light media degradable nonwoven fabrics bag outside sprout-cultivating-bottle seedling method, specifically comprises the following steps:
1) transplanting medium prepares: select perlite, vermiculite, coconut palm chaff and ash to be that Medium Culture is tolerant, respectively Homogeneous phase mixing, and be configured to No. 1 matrix and No. 2 matrix, wherein, No. 1 matrix is: volume ratio is the ash of 1:1:1, perlite and vermiculite; The ash of No. 2 matrix to be volume ratio be 1:1:1:1, perlite, vermiculite and coconut palm chaff; Two kinds of matrix being filled respectively diameter to dress matrix is 5cm, be highly in the non-woven bag of 7cm, neatly put in pallet, soak into matrix with 1000 times of tpns and 1000 times of carbendazim mixed liquors and carry out disinfection, again spray before formal use, to strengthen Disinfection Effect.
2) transition hardening: bottle seedling of being grown thickly by the floral leaf jade hairpin after group training propagation, from airtight hardening 2 ~ 3d group training room is transferred to natural lighting, then to unclamp after lid hardening 1 ~ 2d uncap hardening 2d completely, allows a bottle seedling progressively adapt to external environment condition.
3) seedling plant division is selected: be highly that the floral leaf jade hairpin of 4 ~ 7cm is grown thickly a bottle seedling after choosing propagation, carefully clean the medium of root with running water after taking out from tissue culture bottle, with operating scissors, bottle seedling is divided into individual plant, if there is the budlet just sprouting on individual plant side, budlet can be retained subsidiary by highly suitable seedling of taking root.
4) root-growing agent preparation and root-growing agent process: take root-promoting hormone methyl α-naphthyl acetate NAA and indolebutyric acid IBA in proportion, clear water constant volume after dissolving with a small amount of ethanol.Compound concentration is 200mg/LNAA solution, and 100mg/LIBA+100mg/LNAA mixed liquor two kinds of root-growing agents.Floral leaf jade hairpin seedling base portion 1 ~ 2cm after plant division is immersed in above two kinds of solvents of taking root respectively and takes out after 5s.
5) plantlet in vitro is transplanted: with bamboo let No. 1 and the punching of No. 2 matrix central authorities, the degree of depth is about 1.5cm, and seedling is placed in hole, and finger gently pinches cloth bag, and floral leaf jade hairpin seedling base portion is fully combined with matrix.
6) management after transplanting: rooting treatment and the seedling of taking root after transplanting are placed in Small plastic shed together with pallet, spray 1000 times of wide-spectrum bactericide thiophanate methyls, above take the sunshade net that shading rate is 50% ~ 75%.Taking root first week, all cover with film and sunshade net, air humidity is kept to be 80% ~ 90%, in shed, temperature is 18 ~ 30 DEG C, high temperature can be opened shed two ends and carries out aeration-cooling 1h daytime after one week, can open, if Daily minimum temperature is lower than 8 DEG C when winter temperature is low, big arch shed can be built outside Small plastic shed, be beneficial to insulation.Sunshade net can be opened after taking root gradually and increase illuminance.The every two weeks in midway alternately sprays 800 ~ 1000 times of tpns and 800 ~ 1000 times of carbendazim, and soil humidity is accomplished not do not water.After seedling is substantially all taken root, open sunshade net and film gradually, make seedling adapt to external environment condition completely gradually.
The impact that table 2 different disposal condition is taken root on floral leaf jade hairpin
As shown in Table 2; the condition of the applicable floral leaf jade hairpin outside sprout-cultivating-bottle that the present invention obtains is: floral leaf jade hairpin subculture tufted seedling is divided into individual plant, is carry out culture of rootage in the light environment protection matrix of 1:1:1:1 by implanting ash, vermiculite, perlite and coconut palm chaff volume ratio after quick for base portion 1 ~ 2cm 100mg/LIBA+100mg/LNAA dipping 5s.Rooting rate can reach 96.0%, and number of on average taking root reaches 13.7, and after rooting treatment 40d, effect as shown in Figure 3, can see that the long root system of part has penetrated cloth bag growth.Whole system of taking root not only is beneficial to the growth of jade hairpin self root system explosion type, has environment friendly and pollution-free feature, can reduce production cost simultaneously, and saving group training space, has important practical significance to realizing the extensive nursery of jade hairpin batch production.
Claims (5)
1. a jade hairpin plantlet in vitro Light media degradable nonwoven fabrics outside sprout-cultivating-bottle seedling method, is characterized in that, specifically comprise the following steps:
1) matrix prepares: select perlite, vermiculite, coconut palm chaff or ash to be that Medium Culture is tolerant, Homogeneous phase mixing, is configured to matrix; Matrix is loaded in non-woven bag, neatly put in pallet, soak into matrix with 1000 times of tpns and 1000 times of carbendazim mixed liquors and carry out disinfection, again spray before formal use;
2) transition hardening: bottle seedling of being grown thickly by the jade hairpin after group training propagation, from airtight hardening 2 ~ 3d group training room is transferred to natural lighting, then to unclamp after lid hardening 1 ~ 2d uncap hardening 2d completely, allows a bottle seedling progressively adapt to external environment condition;
3) seedling plant division is selected: bottle seedling of growing thickly after choosing propagation being highly 4 ~ 7cm, carefully clean the medium of root with running water after taking out from tissue culture bottle, with operating scissors, bottle seedling is divided into individual plant, if there is the budlet just sprouting on individual plant side, budlet is retained subsidiary by highly suitable seedling of taking root;
4) root-growing agent preparation and root-growing agent process: take root-promoting hormone in proportion, slowly pours clear water constant volume after dissolving with a small amount of ethanol, and the jade hairpin seedling base portion 1 ~ 2cm after plant division immerses in solvent of taking root and takes out after 5s by preparation root-growing agent;
5) plantlet in vitro is transplanted: with bamboo let in the punching of matrix central authorities, the degree of depth is 1.5cm, and seedling is placed in hole, and finger gently pinches cloth bag, and jade hairpin seedling base portion is fully combined with matrix;
6) management after transplanting: be placed in transparent plastic Small plastic shed after rooting and transplant, spray broad-spectrum germicide, on take sunshade net, taking root first week, all cover with film and sunshade net, keep air humidity, in shed, temperature is 18 ~ 30 DEG C, high temperature is opened shed two ends and is carried out aeration-cooling 1h daytime after one week, need not open when winter temperature is low, open sunshade net after taking root gradually and increase illuminance, midway alternately sprays weekly 800 ~ 1000 times of tpns and 800 ~ 1000 times of carbendazim, and soil humidity is accomplished not do not water; After seedling is substantially all taken root, open sunshade net and film gradually, make seedling adapt to external environment condition completely gradually.
2. jade hairpin plantlet in vitro Light media degradable nonwoven fabrics outside sprout-cultivating-bottle seedling method according to claim 1, it is characterized in that, described step 1) in take root container for size after filling matrix be diameter 5cm, the degradable nonwoven fabrics bag of height 7cm, in soil, the degradable time is 1 year.
3. jade hairpin plantlet in vitro Light media degradable nonwoven fabrics outside sprout-cultivating-bottle seedling method according to claim 1; it is characterized in that; described step 1) in matrix be content be ash, perlite, vermiculite and coconut palm chaff, volume ratio is the light environment protection matrix of 1:1:1:1.
4. jade hairpin plantlet in vitro Light media degradable nonwoven fabrics outside sprout-cultivating-bottle seedling method according to claim 1, is characterized in that, described step 4) in root-growing agent be concentration be 100mg/LIBA+100mg/LNAA mixed liquor, root-growing agent soak time is 5s.
5. jade hairpin plantlet in vitro Light media degradable nonwoven fabrics outside sprout-cultivating-bottle seedling method according to claim 1, is characterized in that, described step 6) in the shading rate of sunshade net be 50% ~ 75%; Described air humidity is 80% ~ 90%, and temperature of taking root is 18 ~ 30 DEG C.
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| CN109792930A (en) * | 2019-01-09 | 2019-05-24 | 安徽省农业科学院农业工程研究所 | A kind of preparation method of azalea potting |
| CN111066504A (en) * | 2019-12-04 | 2020-04-28 | 广州花卉研究中心 | Method for inhibiting 'Pinguanjun anthurium' cluster seedlings from occurring through non-woven fabric substrate block cutting cultivation |
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