CN105494475A - Method for removing impurity liverwort in bletilla acclimating process - Google Patents
Method for removing impurity liverwort in bletilla acclimating process Download PDFInfo
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- CN105494475A CN105494475A CN201510876152.2A CN201510876152A CN105494475A CN 105494475 A CN105494475 A CN 105494475A CN 201510876152 A CN201510876152 A CN 201510876152A CN 105494475 A CN105494475 A CN 105494475A
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- bletilla
- allium
- marchantia
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- 241001313855 Bletilla Species 0.000 title claims abstract description 72
- 238000000034 method Methods 0.000 title claims abstract description 35
- 235000016626 Agrimonia eupatoria Nutrition 0.000 title abstract 5
- 241000196323 Marchantiophyta Species 0.000 title abstract 5
- 239000012535 impurity Substances 0.000 title abstract 2
- 241000234282 Allium Species 0.000 claims abstract description 58
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 18
- 238000002360 preparation method Methods 0.000 claims abstract description 12
- 238000005507 spraying Methods 0.000 claims abstract description 7
- 241000196322 Marchantia Species 0.000 claims description 35
- 239000007921 spray Substances 0.000 claims description 34
- 238000005516 engineering process Methods 0.000 claims description 15
- 239000000463 material Substances 0.000 claims description 12
- 238000000874 microwave-assisted extraction Methods 0.000 claims description 12
- 238000001914 filtration Methods 0.000 claims description 7
- 239000000706 filtrate Substances 0.000 claims description 6
- 235000002732 Allium cepa var. cepa Nutrition 0.000 claims description 4
- 240000002234 Allium sativum Species 0.000 claims description 4
- 235000004611 garlic Nutrition 0.000 claims description 4
- 240000006108 Allium ampeloprasum Species 0.000 claims description 3
- 235000005254 Allium ampeloprasum Nutrition 0.000 claims description 3
- 230000012010 growth Effects 0.000 abstract description 27
- 239000000843 powder Substances 0.000 abstract description 2
- 238000000338 in vitro Methods 0.000 description 12
- 230000000007 visual effect Effects 0.000 description 7
- 239000002689 soil Substances 0.000 description 6
- 241000196324 Embryophyta Species 0.000 description 5
- 201000010099 disease Diseases 0.000 description 5
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 5
- 239000007788 liquid Substances 0.000 description 5
- ODINCKMPIJJUCX-UHFFFAOYSA-N Calcium oxide Chemical compound [Ca]=O ODINCKMPIJJUCX-UHFFFAOYSA-N 0.000 description 4
- 239000003337 fertilizer Substances 0.000 description 4
- 241000238631 Hexapoda Species 0.000 description 3
- 241000607479 Yersinia pestis Species 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 238000009333 weeding Methods 0.000 description 3
- 239000005739 Bordeaux mixture Substances 0.000 description 2
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- 241001494479 Pecora Species 0.000 description 2
- 230000032683 aging Effects 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 239000000292 calcium oxide Substances 0.000 description 2
- 235000012255 calcium oxide Nutrition 0.000 description 2
- 238000004140 cleaning Methods 0.000 description 2
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 description 2
- 238000010899 nucleation Methods 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 239000000047 product Substances 0.000 description 2
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- 238000011160 research Methods 0.000 description 2
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- 229960005486 vaccine Drugs 0.000 description 2
- WURBVZBTWMNKQT-UHFFFAOYSA-N 1-(4-chlorophenoxy)-3,3-dimethyl-1-(1,2,4-triazol-1-yl)butan-2-one Chemical compound C1=NC=NN1C(C(=O)C(C)(C)C)OC1=CC=C(Cl)C=C1 WURBVZBTWMNKQT-UHFFFAOYSA-N 0.000 description 1
- 241000218475 Agrotis segetum Species 0.000 description 1
- TWFZGCMQGLPBSX-UHFFFAOYSA-N Carbendazim Natural products C1=CC=C2NC(NC(=O)OC)=NC2=C1 TWFZGCMQGLPBSX-UHFFFAOYSA-N 0.000 description 1
- 229920002307 Dextran Polymers 0.000 description 1
- 208000000616 Hemoptysis Diseases 0.000 description 1
- 208000032843 Hemorrhage Diseases 0.000 description 1
- 239000005802 Mancozeb Substances 0.000 description 1
- 235000008331 Pinus X rigitaeda Nutrition 0.000 description 1
- 235000011613 Pinus brutia Nutrition 0.000 description 1
- 241000018646 Pinus brutia Species 0.000 description 1
- 241000382353 Pupa Species 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 1
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- VQKFNUFAXTZWDK-UHFFFAOYSA-N alpha-methylfuran Natural products CC1=CC=CO1 VQKFNUFAXTZWDK-UHFFFAOYSA-N 0.000 description 1
- 208000002399 aphthous stomatitis Diseases 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
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- 208000034158 bleeding Diseases 0.000 description 1
- 230000000740 bleeding effect Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 244000144987 brood Species 0.000 description 1
- YYRMJZQKEFZXMX-UHFFFAOYSA-N calcium;phosphoric acid Chemical compound [Ca+2].OP(O)(O)=O.OP(O)(O)=O YYRMJZQKEFZXMX-UHFFFAOYSA-N 0.000 description 1
- 239000002775 capsule Substances 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- JNPZQRQPIHJYNM-UHFFFAOYSA-N carbendazim Chemical compound C1=C[CH]C2=NC(NC(=O)OC)=NC2=C1 JNPZQRQPIHJYNM-UHFFFAOYSA-N 0.000 description 1
- 239000006013 carbendazim Substances 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
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- 150000001875 compounds Chemical class 0.000 description 1
- 239000002537 cosmetic Substances 0.000 description 1
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- 239000004495 emulsifiable concentrate Substances 0.000 description 1
- 239000003995 emulsifying agent Substances 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
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- 238000009413 insulation Methods 0.000 description 1
- JEIPFZHSYJVQDO-UHFFFAOYSA-N iron(III) oxide Inorganic materials O=[Fe]O[Fe]=O JEIPFZHSYJVQDO-UHFFFAOYSA-N 0.000 description 1
- 238000003973 irrigation Methods 0.000 description 1
- 230000002262 irrigation Effects 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 230000035800 maturation Effects 0.000 description 1
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- 239000000203 mixture Substances 0.000 description 1
- 230000003020 moisturizing effect Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 239000002686 phosphate fertilizer Substances 0.000 description 1
- ATROHALUCMTWTB-OWBHPGMISA-N phoxim Chemical compound CCOP(=S)(OCC)O\N=C(\C#N)C1=CC=CC=C1 ATROHALUCMTWTB-OWBHPGMISA-N 0.000 description 1
- 229950001664 phoxim Drugs 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
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- 230000002786 root growth Effects 0.000 description 1
- 230000011218 segmentation Effects 0.000 description 1
- 241000894007 species Species 0.000 description 1
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- 230000035882 stress Effects 0.000 description 1
- 239000000375 suspending agent Substances 0.000 description 1
- NFACJZMKEDPNKN-UHFFFAOYSA-N trichlorfon Chemical compound COP(=O)(OC)C(O)C(Cl)(Cl)Cl NFACJZMKEDPNKN-UHFFFAOYSA-N 0.000 description 1
- 230000002792 vascular Effects 0.000 description 1
- 230000003442 weekly effect Effects 0.000 description 1
- 238000009736 wetting Methods 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/40—Liliopsida [monocotyledons]
- A01N65/42—Aloeaceae [Aloe family] or Liliaceae [Lily family], e.g. aloe, veratrum, onion, garlic or chives
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protection of plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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- Developmental Biology & Embryology (AREA)
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- Botany (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Cell Biology (AREA)
- Ecology (AREA)
- Forests & Forestry (AREA)
- Wood Science & Technology (AREA)
- Agronomy & Crop Science (AREA)
- Toxicology (AREA)
- Microbiology (AREA)
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Abstract
The invention relates to a method for removing an impurity liverwort in a bletilla acclimating process. The method comprises the step of spraying an allium bulb extracting solution when liverwort appears in the bletilla acclimating process, so as to remove liverwort. A preparation method of the allium bulb extracting solution comprises the following steps: smashing allium bulbs; adding water into the allium bulb powder according to the weight ratio of (30 to 50):1, so as to obtain the allium bulb extracting solution. The spraying quantity of the allium bulb extracting solution is 15 to 20 kg/mu or 10 to 15 kg/mu. According to the method provided by the invention, liverwort is killed by adopting the allium bulb extracting solution, the removing time is short, and the extracting solution is natural and pollution-free, and does no harm to growth of bletilla seedlings.
Description
Technical field
The present invention relates to the minimizing technology of foreign material marchantia in a kind of bletilla domestication process, belong to traditional Chinese medicine field of planting.
Background technology
Bletilla is that the orchid family bletilla belongs to herbaceos perennial.For a kind of important traditional Chinese medicine, be mainly distributed in the province such as Yunnan, Guizhou; In addition, also there is a small amount of distribution on the ground such as Anhui, Jiangxi.In several sylvan life all over the whole province height above sea level 1600-2600m in Yunnan, cheuch rock seam, on moist hillside and rock, like warm, shade tolerance is strong.Medicinal pseudobulb, gathered when cauline leaf Huang is withered in October.Cold nature, bitter, hinders hemoptysis for lung, traumatism and bleeding, canker sores etc.In recent years, bletilla, except making medicine materical crude slice and directly using, is also widely used in the compatibility of herbal mixture.Along with people utilize the research and development of bletilla, now many novelty teabag and new product are developed, as " appetizing sheet ", vascular occlusive agent, bletilla capsule, Compound Burn Spray, the bletilla dextran made with bletilla gluing segment, the adhesive etc. made with its starch.Bletilla can also make emulsifier, suspending agent, is also widely used in these external cosmetics and health products.Due to more than, the continuous minimizing of bletilla wild resource, output is just in downward trend year by year, and the market demand of bletilla is in recent years increasing, and price also constantly rises, and market price rises to about 50 yuan by original per kilogram 4-5 yuan.According to statistics, the bletilla raw material of domestic market demand exceedes a few kiloton, and the natural quantity delivered of reality only has hundreds of ton, gives amount wretched insufficiency.Along with many, whole nation pharmacy corporation increases bletilla raw materials requirement amount, many pharmaceutical factories source of goods is not enough, and source of goods has turned to other kind bletilla outside the province.Estimate at a few years from now on, between supply and demand, breach can constantly widen, and situation of sale can turn to strong sales from current situation of selling well, and price yet can climb up New step.Therefore, bletilla introduction and acclimatization and standardized planting have become and have solved the inevitable choice that bletilla natural resources is deficient, improve interior quality, the introduction and acclimatization of bletilla and large-scale planting thereof and demonstration imperative.The wild bletilla degree of meeting with of China's most area is excavated in recent years, causes its wild natural resources sharply to reduce, endangered, is classified as lays special stress on protecting one of Wild Medicinal by country.Bletilla seed is tiny of endosperm, difficult germination and growth under natural situation, and the cultivation of seedling is comparatively difficult.Due to huge market demand, the bletilla market price is in sharply soaring gesture in recent years.Therefore, the research accelerating bletilla artificial propagation and planting technology is imperative.At present, about the breeding of bletilla plantlet in vitro, there are reports, but have no the report for training tissue culture seedling technical research.Marchantia is a kind of Harmful species in bletilla domestication process of growth, its harm: absorption domestication process claims the nutrient in soil, suppresses the growth of bletilla seedling.Marchantia large area is bred, and be attached to soil surface and soil dissolved oxygen amount is reduced, bletilla belongs to the minimizing of root aerial root dissolved oxygen and root aerobic respiration can be impelled to weaken, and soil carbon dioxide increases formation acid and indirectly causes rotten of plant even dead.The existing method killing marchantia is that copper sulphate and quicklime are made into bordeaux mixture sprinkling, and shortcoming is: by stoping marchantia spore maturation to kill marchantia, and length consuming time often, suppresses seedling growth aging, can not kill marchantia completely.
Summary of the invention
The invention provides the minimizing technology of foreign material marchantia in a kind of bletilla domestication process, solve the existing method length consuming time killing marchantia often, suppress seedling growth aging, the problem of marchantia can not be killed completely.
For solving the problems of the technologies described above, the technical solution used in the present invention is:
A minimizing technology for foreign material marchantia in bletilla domestication process, in bletilla domestication process in there is marchantia, sprays allium bulb extract, removes marchantia.
Further, a preferred embodiment of the present invention: described allium is onion, garlic, verdant or leek.
Further, a preferred embodiment of the present invention: the preparation method of described allium bulb extract: pulverized by allium bulb, then according to weight ratio 1:30-50, add water, both obtained allium bulb extract.
Further, a preferred embodiment of the present invention: the preparation method of described allium bulb extract: allium bulb is pulverized for 200-300 order, then according to weight ratio 1:30-50, add water, Microwave Extraction 0.5-1h, the filtrate of filtration, both obtained allium extract.
Further, a preferred embodiment of the present invention: described Microwave Extraction is microwave power 1300-1500W, frequency: 1500-2000Hz, time 15-20min, temperature 30-50 degree.
Further, a preferred embodiment of the present invention: the amount of spraying of described allium extract is 15-20kg/ mu or 10-15kg/ mu.
Beneficial effect of the present invention:
Method of the present invention adopts allium bulb extract to go to kill marchantia, and the time is fast, and extract is natural pollution-free right, on the growth of bletilla seedling without impact.
Embodiment
Be clearly and completely described to the technical scheme in the embodiment of the present invention below, obviously, described embodiment is only the present invention's part embodiment, instead of whole embodiments.Based on the embodiment in the present invention, those of ordinary skill in the art, not making the every other embodiment obtained under creative work prerequisite, belong to the scope of protection of the invention.
Embodiment 1
The minimizing technology of foreign material marchantia in a kind of bletilla domestication process, bletilla domestication adopts Tissue vaccine to cultivate, specific as follows: to transplant when plantlet in vitro grows to 3 ~ 5cm, ensure hardening early stage air humidity between 80 ~ 90%, need plantlet in vitro to be placed in 12 hours lower sunshine of natural daylight before bottle outlet, in-bottle seeding about 3d, then seedling is taken out from medium, seedling will be polluted separate with qualified seedling and clean, qualified seedling running water cleaning twice, pollute Miao Zexian clean in running water after be soak in 0.3% carbendazim in concentration again, cleaning process is wanted rapidly and light and fast.
Then by plantlet in vitro field planting on seedbed, the temperature and humidity will controlled well in booth after field planting; Will close window insulation when temperature is low, loose temperature of windowing during temperature height, watering in canopy, the watering time is after at 15 in afternoon, and watering adopts sprinkling irrigation formula, and canopy humidity is not easily too high, and greenhouse temperature should control between 15 DEG C ~ 35 DEG C, and humidity duty controls between 50 ~ 70%;
Fertilising, field planting, after 15 days, executes sheep excrement every two weeks, sheep excrement must process by fermentation, not so can cause burning seedling, sprays weekly the foliage fertilizer of 0.1% ~ 0.2% after seedling survives, impose composite fertilizer, high-temperature time will be circumvented during fertilising and select 15 later fertilization effects better.
The hardening phase will note the extermination of disease and insect pest, bletilla plantation does not find disease, only finds that main insect pest is soil insect black cutworm.Sting food with larva or bite bletilla seedling and tender shoots broken, affecting bletilla growth.There are for 3 ~ 4 generations in 1 year in disease feature, survives the winter with larva and pupa.3 ~ April is that adult moth appearance contains the phase.4 ~ May is that first brood of larvae is caused harm the heaviest period.Cause harm later and alleviate gradually.Prevention and controls 80% chlorophos wetting powder, 800 times of liquid or 50% phoxim emulsifiable concentrate, 1000 times of liquid irrigating roots, spraying time is in the afternoon after 6, and disease control will be accomplished early to find early control.
In plantlet in vitro field planting process, there is marchantia in seedbed, affect the growth of bletilla seedling.Spray allium extract in time.The preparation method of allium bulb extract: pulverized by onion, then according to weight ratio 1:50, add water, both obtained allium bulb extract.Then then will dilute 10 times by allium bulb, according to 20kg/ mu, spray.
Once, spray in the morning in spray, and afternoon, visual effects, affected bletilla seedling growth nothing.
Embodiment 2
The minimizing technology of foreign material marchantia in a kind of bletilla domestication process, bletilla domestication adopts Tissue vaccine to cultivate, specific as follows: the stem tuber filtering out tender shoots from wild bletilla, adjoins to deliver a child cut from old bulb, segmentation pseudobulb was planted annual March, open cave according to each 10cm of seeding row spacing, dark 10cm, every cave plants 1, sprouting upwards, bletilla is put after in ethnic group plant hole, be mixed with fertilizer fine earth cover, then with pine needle cover, moisturizing.Field management: (1) weeding: bletilla plant is short and small, extirpate weeds in time.First time after April bletilla tidy seedlings output, second time June bletilla for a long time prosperous, third time, the 4th time in October in August.Intertill appropriate to the occasion shallow, uproot loose furrow face soil, shovel is weeds to the greatest extent, avoid hindering its root.(2) apply fertilizer: after tidy seedlings output, impose urea 75kg/hm in conjunction with weeding
2once, at growth animated period, normal superphosphate 600kg/hm is imposed in conjunction with weeding
2, rhizome is expanded fast, and active principle content improves greatly.General cultivation is bloomed for latter 2 months, and Hua Houzhi executes 1 phosphate fertilizer mid-August, and block root growth can be made to enrich.(3) water management: bletilla likes shady and cool moist environment, avoids flood, milpa will keep moistening, and will often water during weather arid, rainy season will dredge ditch water discharge in time, prevents ponding from causing stem tuber to rot.(4) extermination of disease and insect pest: often rust occurs, their early stage can be sprayed, or prevent and treat with sulfonat spraying with 25% triadimefon, 600 ~ 800 times of liquid, 62.25% celestial raw 600 times of liquid, 80% mancozeb, 600 ~ 800 times of liquid.
In domestication process, there is marchantia, affect the growth of bletilla seedling.After 3 months, there is marchantia in seedbed to booth in plantlet in vitro field planting, affect the growth of bletilla seedling.Spray allium extract in time.The preparation method of allium bulb extract: pulverized by garlic, then according to weight ratio 1:30, add water, both obtained allium bulb extract.Then then will dilute 10 times by allium bulb, according to 15kg/ mu, spray.
Once, spray in the morning in spray, and afternoon, visual effects, affected bletilla seedling growth nothing.
Embodiment 3
Substantially the same manner as Example 1, difference is: in plantlet in vitro incubation, marchantia appears in seedbed, affects the growth of bletilla seedling.Spray allium extract in time.The preparation method of allium bulb extract: by verdant pulverizing, then according to weight ratio 1:40, add water, both obtained allium bulb extract.Then then will dilute 10 times by allium bulb, according to 18kg/ mu, spray.
Once, spray in the morning in spray, and afternoon, visual effects, affected bletilla seedling growth nothing.
Embodiment 4
Substantially the same manner as Example 2, difference is: in plantlet in vitro field planting process, marchantia appears in seedbed, affect the growth of bletilla seedling.Spray allium extract in time.The preparation method of allium bulb extract: pulverize leek for 200-300 order, then according to weight ratio 1:30, add water, Microwave Extraction 0.5h, the filtrate of filtration, both obtained allium extract.Microwave Extraction is microwave power 1300W, frequency: 2000Hz, temperature 50 degree.Then will dilute 15 times by allium bulb, according to 10kg/ mu, spray.
Once, spray in the morning in spray, and afternoon, visual effects, affected bletilla seedling growth nothing.
Embodiment 5
Substantially the same manner as Example 2, difference is: in domestication process, occur marchantia, affects the growth of bletilla seedling.After 3 months, there is marchantia in seedbed to booth in plantlet in vitro field planting, affect the growth of bletilla seedling.Spray allium extract in time.The preparation method of allium bulb extract: be 200-300 order by onion, then according to weight ratio 1:50, adds water, Microwave Extraction 0.5h, the filtrate of filtration, both allium extract.Microwave Extraction is microwave power 1500W, frequency: 1800Hz, temperature 30 degree.Then will dilute 15 times by allium bulb, according to 15kg/ mu, spray.
Once, spray in the morning in spray, and afternoon, visual effects, affected bletilla seedling growth nothing.
Embodiment 6
Substantially the same manner as Example 1, difference is: in plantlet in vitro field planting process, marchantia appears in seedbed, affect the growth of bletilla seedling.Spray allium extract in time.The preparation method of allium bulb extract: pulverize garlic for 200-300 order, then according to weight ratio 1:40, add water, Microwave Extraction 0.8h, the filtrate of filtration, both obtained allium extract.Microwave Extraction is microwave power 1400, frequency: 2000Hz, temperature 40 degree.Then will dilute 15 times by allium bulb, according to 13kg/ mu, spray.
Once, spray in the morning in spray, and afternoon, visual effects, affected bletilla seedling growth nothing.
Embodiment 7
Substantially the same manner as Example 1, difference is: in plantlet in vitro field planting process, marchantia appears in seedbed, affect the growth of bletilla seedling.Spray allium extract in time.The preparation method of allium bulb extract: be 200-300 order by verdant pulverizing, then according to weight ratio 1:50, adds water, Microwave Extraction 0.5h, the filtrate of filtration, both allium extract.Microwave Extraction is microwave power 1300W, frequency: 1500Hz, temperature 30 degree.Then will dilute 15 times by allium bulb, according to 10kg/ mu, spray.
Once, spray in the morning in spray, and afternoon, visual effects, affected bletilla seedling growth nothing.
Comparative example 1
Substantially identical with embodiment, difference is: in plantlet in vitro field planting process, marchantia appears in seedbed, affect the growth of bletilla seedling.The concrete proportioning of configuration bordeaux mixture is as follows: copper sulphate: quicklime: water is directly spray after the solution of 1:0.7:200 times of ratio is accompanied, and once, spraying 4-5 time can eliminate the 15 days sprays in interval.
But inhibit the growth of bletilla seedling.
The foregoing is only preferred embodiment of the present invention, not in order to limit the present invention, all any amendments, equivalent replacement etc. done in protection scope of the present invention, all should be included within protection scope of the present invention.
Claims (6)
1. the minimizing technology of foreign material marchantia in bletilla domestication process, is characterized in that: in bletilla domestication process in there is marchantia, spray allium extract, removes marchantia.
2. the minimizing technology of foreign material marchantia in a kind of bletilla domestication process according to claim 1, is characterized in that: described allium is onion, garlic, verdant or leek.
3. the minimizing technology of foreign material marchantia in a kind of bletilla domestication process according to claim 1 and 2, it is characterized in that: the preparation method of described allium extract: allium is pulverized for 300-500 order, then according to weight ratio 1:30-50, add water, both obtained allium extract.
4. the minimizing technology of foreign material marchantia in a kind of bletilla domestication process according to claim 1 and 2, it is characterized in that: the preparation method of described allium extract: allium bulb is pulverized for 200-300 order, then according to weight ratio 1:30-50, add water, Microwave Extraction 0.5-1h, the filtrate of filtering, both obtained allium extract.
5. the minimizing technology of foreign material marchantia in a kind of bletilla domestication process according to claim 4, is characterized in that: described Microwave Extraction is microwave power 1300-1500W, frequency: 1500-2000Hz, time 15-20min, temperature 30-50 degree.
6. the minimizing technology of foreign material marchantia in a kind of bletilla domestication process according to claim 1, is characterized in that: the amount of spraying of described allium extract is 15-20kg/ mu.
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