CN105494447B - Biological seed coating agent and its preparation method and application for controlling soybean root disease - Google Patents
Biological seed coating agent and its preparation method and application for controlling soybean root disease Download PDFInfo
- Publication number
- CN105494447B CN105494447B CN201511009463.5A CN201511009463A CN105494447B CN 105494447 B CN105494447 B CN 105494447B CN 201511009463 A CN201511009463 A CN 201511009463A CN 105494447 B CN105494447 B CN 105494447B
- Authority
- CN
- China
- Prior art keywords
- seed coating
- coating agent
- soybean
- bacterial strain
- biological seed
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/10—Animals; Substances produced thereby or obtained therefrom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/06—Coating or dressing seed
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/02—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents
- A01N25/04—Dispersions, emulsions, suspoemulsions, suspension concentrates or gels
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/02—Sulfur; Selenium; Tellurium; Compounds thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/14—Boron; Compounds thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/16—Heavy metals; Compounds thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/16—Heavy metals; Compounds thereof
- A01N59/20—Copper
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/26—Phosphorus; Compounds thereof
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Chemical & Material Sciences (AREA)
- Inorganic Chemistry (AREA)
- Soil Sciences (AREA)
- Dispersion Chemistry (AREA)
- Toxicology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Virology (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The present invention provides a kind of biological seed coating agents for controlling soybean root disease, including mass ratio is 0.02-0.15%CuSO4, 0.05-0.26% (NH4)6Mo7O24·4H2O, 0.01-0.05%H3BO3, 0.1-0.5%MnSO4, 0.04-0.25%FeSO4, the KH of 1.5-6%2PO4, 0.02-1%K2SO4, 2-8% dispersants, 2-5% film forming agents, 0.1-1.2% warning coloration, 0.95-2.65% binders, remaining is biocontrol bacterial strain zymotic fluid.Biological seed coating agent provided by the invention is coated use to soya seeds, can have the function that while inhibit soybean cyst nematode and root rot, to effectively prevent soybean root disease, and soybean plant strain can be promoted to grow, and improves yield.
Description
Technical field
The invention belongs to microbial pesticide technical fields, and in particular to a kind of control soybean cyst nematode Heterodera glycines and root rot
Biological seed coating agent, and its preparation method and application.
Background technology
Soybean cyst nematode is caused by being infected by soybean cyst nematode Heterodera glycines (Soybean Cyst Nematode, SCN),
One of the important disease of world soybean production is endangered, soybean cyst nematode can occur in soybean entire breeding time, predominantly
Evil host's root system, is a kind of soil-borne disease of extremely difficult prevention, often causes heavy losses to Soybean production.The disease generally makes greatly
The beans underproduction 10%~30%, serious plot is up to 70%~90%, or even causes to have no harvest, caused by disease more single than any type
Harm it is all big.Currently, all reporting the generation of the disease in almost all of soybean producing region.In China, Heilungkiang, Jilin, the Liao Dynasty
Rather, (autonomous region is directly under the jurisdiction of for the province such as the Inner Mongol, Hebei, Henan, Shandong, Shanxi, Shaanxi, Anhui, Hubei, Jiangsu, Beijing, Shanghai
City) there is generation.In terms of world wide, the harm and sprawling of soybean cyst nematode have the tendency that increasingly aggravating, related soybean
The research of cyst nematode is increasingly paid attention to by various countries.
Root rot be it is a kind of it is distributed more widely, that harm is serious, Species of Pathogens is various and prevention is more difficult is important
Soil-borne disease, it is considered to be the principal element of limitation soybean yields in global range.The generation of this disease can make soybean plant strain
Root weight, root nodule weight, nitrogenase activity and lateral root number are influenced to different extents, and soybean quality is made to be decreased obviously.
Root rot causes the soybean underproduction, the general underproduction 10%~30%, up to 60%, or even total crop failure when serious throughout the year according to investigations.In recent years
Come being continuously increased with soybean area, meeting next batch of soybean situation is increasingly sharpened so that and the harm of root rot aggravates year by year,
Influence to yield is also increasing, and economic loss reaches several hundred million members caused by root rot every year for country.
However lack the measure of effectively control soybean cyst nematode evil and soybean root rot disease at present.Although efficent rotation
It is a kind of Main Agricultural control measure, but the time needed is longer, can be only achieved dynamic equilibrium after general 12 years, therefore, production
On be difficult to realize；Although chemical agent is capable of the generation of cost-effective control disease, there are problems that serious " 3R ".
At present China's major soybean production areas prevention seedling stage diseases and pests using it is more be seed coat agent, main insecticidal constituent is furans
Pellet, but the medicine is disabled, therefore the prevention of pest and disease damage is turned to biological control by people, is found and is developed effectively, to environment
Friendly pharmaceutical preparations become inevitable, and seed coat agent is usually made of active constituent and non-active ingredient, and active constituent includes desinsection
Agent, fungicide, plant growth regulator, fertilizer, nutrient, beneficial microbe and its product and biological pesticide etc.；It is non-live
Property ingredient refer to film forming agent and corresponding auxiliary aid in seed coat agent, be the physics and chemical property for maintaining seed coat agent, control clothing
The important component that active constituent is sustained in film.Biological seed coating agent action principle is mainly to promote the fertility of crop ambient enviroment, system
It makes nutrition and assists plant absorption nutrition, while improving the nutritional capacity of plant itself.In this environment, beneficial microbe and
Its metabolin can survive and keep its original activity, and the mesh of prevention pest and disease damage is reached by antagonism and Competition
's.
Country's biological seed coating agent variety protection is single at present, and large-scale production technology falls behind, although having developed, having registered not
Few biological pesticide kind, but the production level of many kinds is low, and preventive effect efficiency is low, in terms of microbial pesticide ferments kind still
Optimization is not achieved and accurately controls production；In post-processing and medicament development, fail the soybean for surrounding soybean root disease well
Cyst nematode and root rot are prevented, to advantageously promote plant strain growth.
Invention content
The purpose of the present invention is obtaining a kind of biological seed coating agent that can prevent soybean root Major Diseases, will have and kill line
Active bacterial strain routinely after liquid fermentation and culture, is pressed certain with the zymotic fluid of strains tested and other chemical elements, auxiliary agent etc.
The processing of ratio mixture, forms biological seed coating agent, to be coated soya seeds, reaches effectively control soybean cyst nematode and soybean
Root rot promotes Soybean Seedlings Growth, improves the purpose of yield.
For this purpose, the present invention provides a kind of biological seed coating agent for controlling soybean root disease, including following component, respectively
Component is prepared by mass percentage,
0.02%~0.15% CuSO4, 0.05%~0.26% (NH4)6Mo7O24·4H2O, 0.01%~0.05%
H3BO3, 0.1%~0.5% MnSO4, 0.04%~0.25% FeSO4, 1.5%~6% KH2PO4, 0.02%~
1.0% K2SO4, 2%~8% dispersant, 2%~5% film forming agent, 0.1%~1.2% warning coloration, 0.95%~
2.65% binder, remaining is with killing the active biocontrol bacterial strain zymotic fluid of line.
Preferably, biological seed coating agent provided by the invention includes following component, and each component is prepared by mass percentage：
0.04% CuSO4, 0.08% (NH4)6Mo7O24·4H2O, 0.02% H3BO3, 0.2% MnSO4, 0.22%
FeSO4, 5% KH2PO4, 0.05% K2SO4, 6% dispersant, 4.0% film forming agent, 1% warning coloration, 2.55%
Binder, remaining is with killing the active biocontrol bacterial strain zymotic fluid of line.
Preferably, the biocontrol bacterial strain zymotic fluid is simple fermentation of bacillus liquid.
It is obtained it is highly preferred that the simple bacillus detaches from soybean root system soil.
Preferably, the dispersant is calcium lignosulfonate；The film forming agent is methylcellulose；The warning coloration is hole
Sparrow is green；The binder is the mixture of the xanthans that mass percent is 1.5-20: 100 and converted starch composition.
Preferably, the biocontrol bacterial strain zymotic fluid be by bacterial strain to be seeded after test tube strains culture, then through expand send out
Made from ferment culture, specific preparation method is as follows：
It is respectively 0.2%~0.4% beef extract by weight percent, 0.4%~0.6% peptone, 0.1%~
0.2% yeast extract, 0.8%~1% sucrose, 1.2%~1.8% agar, remaining is distilled water, is adjusted after being uniformly mixed
It is 7 to save pH, and simple Bacillus strain to be seeded is inoculated on the slant medium, is placed in by bevel culture medium
25 DEG C of constant temperature incubations 2 days obtain test tube strains；
It is respectively 0.2%~0.4% beef extract by weight percent, 1.0%~1.2% peptone, 0.5%~
1% yeast extract, 1%-1.5% sucrose and excess water mixing adjust after pH is 6.8~7 and liquid fermentation and culture are made later
Base；
The test tube strains are inoculated into the liquid fermentation medium, at 25 DEG C~28 DEG C, with rotating speed
150r.min-1~200r.min-1In shaking table shaken cultivation for 24 hours~72h, obtain the biocontrol bacterial strain zymotic fluid.
The present invention also provides a kind of preparation methods for controlling the biological seed coating agent of soybean root disease, specifically,
It is respectively 0.02%~0.15% CuSO by weight percent4, 0.05%~0.26% (NH4)6Mo7O24·4H2O,
0.01%~0.05% H3BO3, 0.1%~0.5% MnSO4, 0.04%~0.25% FeSO4, 4%~6%
KH2PO4With 0.02%~0.08% K2SO4Attrition grinding after mixing, adds 2%~8% dispersant, 2%~5%
Film forming agent, 0.1%~1.2% warning coloration, 0.95%~2.65% binder, remaining is with killing the active biocontrol microorganisms of line
Strain zymotic fluid, said components stir into uniform viscous fluid, and oscillation is uniformly mixed, and prepares the biological seed coating agent.
Preferably, the CuSO for being 0.04% by quality accounting4, 0.08% (NH4)6Mo7O24·4H2O, 0.02%
H3BO3, 0.2% MnSO4, 0.22% FeSO4, 5% KH2PO4With 0.05% K2SO4Attrition grinding after mixing, adds
6% dispersant, 4% film forming agent, 1% warning coloration, 2.55% binder, remaining is with killing the active biocontrol microorganisms of line
Strain zymotic fluid, said components stir into uniform viscous fluid, and oscillation is uniformly mixed, and prepares the biological seed coating agent.
Biological seed coating agent made from preparation method of the present invention is for coating soya seeds to be sowed, in use, will
The biological seed coating agent that mass percent is 1-5: 70 is mixed with soya seeds, and biological seed coating agent is made to be uniformly wrapped on Soybean Species sublist
Face can be sowed after drying.
Since simple bacillus is to the Biocontrol Effect of soybean cyst nematode Heterodera glycines and root rot, biology provided by the invention
Seed coat agent using detaching the simple bacillus (Sneb545) obtained from soybean nodulation, zymotic fluid after fermented and cultured and its
The mixtures such as his chemical element, auxiliary agent, are made seed coat agent, are coated use to soya seeds, can reach while inhibit soybean
The effect of Cyst nematode and root rot to effectively prevent soybean root disease, and can promote soybean plant strain to grow, and improve production
Amount.
Specific implementation mode
In order to enable those skilled in the art to more fully understand, technical scheme of the present invention is practiced, with reference to specific
The invention will be further described for embodiment, but illustrated embodiment is not as a limitation of the invention.
Embodiment 1
It is a kind of for controlling the biological seed coating agent of soybean root disease, including weight percent is 90.4% to have and kill line
Active simple fermentation of bacillus liquid；And weight percent is respectively 0.09% CuSO4, 0.18% (NH4)6Mo7O24·4H2O, 0.04% H3BO3, 0.3% MnSO4, 0.04% FeSO4, 1.5% KH2PO4, 1% K2SO4,
3% calcium lignosulfonate, 2% methylcellulose, 0.5% peacock green, and become by 0.15% xanthans and 0.8%
Property starch composition binder, above-mentioned each component prepares by mass percentage.
Specifically preparation process is：By above-mentioned 0.09% CuSO4, 0.18% (NH4)6Mo7O24.4H2O, 0.04%
H3BO3, 0.3% MnSO4, 0.04% FeSO4, 1.5% KH2PO4, 1% K2SO4Attrition grinding after mixing, adds
3% calcium lignosulfonate, 2% methylcellulose, 0.5% peacock green, and become by 0.15% xanthans and 0.8%
Property starch composition binder and the 90.4% simple fermentation of bacillus liquid stir into uniform viscous fluid, vibrate
It is uniformly mixed, prepares the biological seed coating agent.The mixing ratio of obtained biological seed coating agent and grain weight is 1: 70, uniformly
It is wrapped in soya seeds surface, can be sowed after drying.
Wherein, above-mentioned to have that kill the active simple fermentation of bacillus liquid of line be to train bacterial strain to be seeded through test tube strains
After supporting, then through expanding made from fermented and cultured, specific preparation method is as follows：
It is respectively 0.3% beef extract, 0.5% peptone, 0.1% yeast extract, 1% sugarcane by weight percent
Sugar, 1.5% agar, remaining is distilled water, and it is 7 that pH is adjusted after being uniformly mixed, bevel culture medium, by letter to be seeded
Single Bacillus strain is inoculated on the slant medium, is placed in 25 DEG C of constant temperature incubations 2 days, obtains simple bacillus test tube
Strain.
It is respectively 0.2% beef extract by weight percent again, 1% peptone, 0.5% yeast extract, 1.0%
Sucrose, remaining is distilled water, and said components adjust after pH is 6.8 after being sufficiently mixed and simple fermentation of bacillus culture medium is made.
The simple bacillus test tube strains are inoculated into liquid simple fermentation of bacillus culture medium, 28
At DEG C, with rotating speed 150rmin-1The shaken cultivation 48h in shaking table obtains simple fermentation of bacillus liquid.
The all analytical reagents bought on the market of above-mentioned chemical reagent, the simple bacillus used in of the invention
(Bacillus simplex) sneb545 is detached from soybean root system soil to be obtained, on October 11st, 2012 is deposited in
State's Microbiological Culture Collection administration committee common micro-organisms center, preserving number are CGMCC No.6650.
Specifically, above-mentioned simple fermentation of bacillus liquid can carry out large-scale production by large fermentation tank, by simple bud
The fermentation tank that spore bacillus is inoculated into 50L~100L ferments, and is cultivated at 25 DEG C, initial pH8.0, fermentation time 48h.Hair
Activity of the zymotic fluid to target nematode is surveyed sample during ferment, and the tank time is put according to the determination of activity criteria's curve.
Embodiment 2
It is a kind of for controlling the biological seed coating agent of soybean root disease, including 80.84% has that kill line active simple
Fermentation of bacillus liquid, wherein 0.04% CuSO4, 0.08% (NH4)6Mo7O24·4H2O, 0.02% H3BO3, 0.2%
MnSO4, 0.22% FeSO4, 5% KH2PO4, 0.05% K2SO4, 6.0% calcium lignosulfonate, 4% methyl fibre
Dimension is plain, 1% peacock green, and the binder being mixed to form by 0.05% xanthans and 2.5% converted starch, above-mentioned each group
Divide and prepares by mass percentage.
Specifically preparation process is：By above-mentioned 0.04% CuSO4, 0.08% (NH4)6Mo7O24·4H2O, 0.02%
H3BO3, 0.2% MnSO4, 0.22% FeSO4, 5% KH2PO4, 0.05% K2SO4, attrition grinding after mixing, then add
Enter 6% calcium lignosulfonate, 4% methylcellulose, 1.0% peacock green, and by 0.05% xanthans and 2.5%
The fermentation broth agitation of the binder of converted starch composition and the 80.84% simple Bacillus strain is at uniform viscous
Magma, oscillation are uniformly mixed, and prepare the biological seed coating agent.The ratio of obtained biological seed coating agent and grain weight is 3:
70, it is uniformly wrapped in soya seeds surface, can be sowed after drying.
Wherein, above-mentioned with the active bacterial strain fermentation liquor of line is killed as simple fermentation of bacillus liquid, specific preparation method is such as
Under：
It is respectively 0.2% beef extract by weight percent, 0.4% peptone, 0.2% yeast extract, 0.8%
Sucrose, 1.6% agar, remaining is distilled water, and it is 7.0 to adjust pH after being uniformly mixed, and bevel culture medium will be to be seeded
Simple bacillus be inoculated on the slant medium, be placed in 25 DEG C of constant temperature incubations 2 days, obtain the test tube strains of bacterial strain.
It is being respectively 0.3% beef extract by weight percent, 1.2% peptone, 0.8% yeast extract,
1.2% sucrose, remaining is distilled water, and said components adjust the liquid fermented and cultured that bacterial strain is made after pH is 7.0 after being sufficiently mixed
Base.
The simple Bacillus is inoculated into liquid simple fermentation of bacillus culture medium, at 28 DEG C
Under, with rotating speed 180rmin-1Shaken cultivation for 24 hours, obtains the zymotic fluid of bacterial strain in shaking table.
Embodiment 3
It is a kind of for controlling the biological seed coating agent of soybean root disease, including 84.7% has and kills the active simple bud of line
Spore bacillus fermentation liquid, wherein 0.12% CuSO4, 0.25% (NH4)6Mo7O24·4H2O, 0.05% H3BO3, 0.4%
MnSO4, 0.06% FeSO4, 2.2% KH2PO4, 1% K2SO4, 5% calcium lignosulfonate, 2.8% Methyl cellulose
Element, 1% peacock green, and the binder that is made of 0.12% xanthans and 2.3% converted starch, above-mentioned each component press matter
Percentage is measured to prepare.Specifically preparation process is：By above-mentioned 0.12% CuSO4, 0.25% (NH4)6Mo7O24·4H2O,
0.05% H3BO3, 0.4% MnSO4, 0.06% FeSO4, 2.2% KH2PO4, 1% K2SO4, crush and grind after mixing
Mill, adds 5% calcium lignosulfonate, 2.8% methylcellulose, 1% peacock green, and by 0.12% xanthans
It is stirred into uniformly with the binder of 2.3% converted starch composition and 84.7% mixed fermentation liquid of three kinds of bacterial strains
Viscous fluid, oscillation are uniformly mixed, and prepare the biological seed coating agent.The ratio of obtained biological seed coating agent and grain weight is 5:
70, it is uniformly wrapped in soya seeds surface, can be sowed after drying.
Wherein, above-mentioned with the active bacterial strain fermentation liquor of line is killed as simple fermentation of bacillus liquid, specific preparation method is such as
Under：
It is respectively 0.4% beef extract, 0.6% peptone, 0.2% yeast extract, 1% sugarcane by weight percent
Sugar, 1.8% agar, remaining is distilled water, and it is 7 that pH is adjusted after being uniformly mixed, bevel culture medium, by letter to be seeded
Single bacillus is inoculated on the slant medium, is placed in 25 DEG C of constant temperature incubations 2 days, obtains the test tube strains of bacterial strain.
It is being respectively 0.4% beef extract by weight percent, 1.2% peptone, 1.0% yeast extract,
1.5% sucrose, remaining is distilled water, and said components adjust the liquid fermented and cultured that bacterial strain is made after pH is 7.0 after being sufficiently mixed
Base.
The simple Bacillus is inoculated into liquid simple fermentation of bacillus culture medium, at 25 DEG C
Under, with rotating speed 200rmin-1The shaken cultivation 72h in shaking table obtains the zymotic fluid of bacterial strain.
Embodiment 4：
To through implementing to fall biological seed coating agent treated soya seeds made from 1 and embodiment 2 respectively in 2012 in the Liao Dynasty
The Kangping Counties Ning Sheng, Heilongjiang Province Daqing carry out field plot sowing test.Plot experiment takes the side of 5 repetitions of random district's groups
Method.5 row areas of each processing, row long 2.5m, line-spacing 0.65m, processing interval 1m, plot area 8.1m2, gross area 450m2.With not
The seed of soya seeds and commodity the seed coat agent processing of coating is respectively as comparison.Wherein, commodity seed coat agent is that BFA continuous cropping is red,
Production unit：Middle Inner Mongol Mo Qi branch companies of wealth flourishing age (Beijing) biotechnology Development Co., Ltd, main component is biological species
Clothing agent " BFA " 20%, standard code：Q/CHS03-2001.
The variation of 1 soybean biological seed coating agent processed soybeans root of table outer cyst and Gen Nei nematode populations
Note：Letter is that Duncan's multiple range tests indicate poor respectively as a result, different size writes English alphabet after number
Heteropolar significantly (p < 0.01) and significant difference (p < 0.05), following table is same.
The soya seeds that experimental data as shown in Table 1 can be seen that the biological seed coating agent processing of the preparation of the present embodiment 1 exist
Kangping, grand celebration preventive effect cyst inhibiting rate on root is respectively increased compared with the soya seeds of blank control not being coated
35.36% and 39.29%, the soya seeds of biological seed coating agent processing prepared by the present embodiment 2 are in Kangping, preventive effect and the sky of grand celebration
The soya seeds not being coated compareed in vain are compared has been respectively increased 49.7% and 54.2% to cyst inhibiting rate on root, and difference is aobvious
It writes；Also reach the significance level of difference with the soya seeds of commodity seed coat agent processing.The biological seed coating agent that the embodiment of the present invention 1 provides
The root interior lines borer population of the soya seeds of processing is compared with the soya seeds of blank control not being coated, to nematode inhibiting rate in root point
60.93% and 40.2% are not improved, and the root interior lines of the soya seeds for the biological seed coating agent processing that the embodiment of the present invention 2 provides
77.23% He has been respectively increased compared with the soya seeds of blank control not being coated, to nematode inhibiting rate in root in borer population
55.1%.Meanwhile the soya seeds of biological seed coating agent processing provided by the invention can also significantly inhibit cyst quantity in soil
Proliferation, implement fall 1 compared with the soya seeds of blank control not being coated difference reach the pole level of signifiance, inhibiting rate is respectively increased
36.60% and 37.66%, the difference compared with the soya seeds of blank control not being coated of embodiment 2 reaches the pole level of signifiance,
54.6% and 57.23% has been respectively increased in inhibiting rate.Since Kangping is different with the field plot incidence of grand celebration, nematode microspecies
Difference, it is possible that result is caused to slightly have difference.
Influence of 2 soybean biological seed coating agent of table to plant strain growth
Pass through data analysis shown in table 2, it can be seen that in seedling stage, biological species prepared by Example 1 and Example 2 of the present invention
Clothing agent can safely and effectively promote the growth of soybean seedling；In the soybean mature phase to Kangping and grand celebration proving ground using the present invention
After the soya seeds nursery of biological seed coating agent processing prepared by embodiment 1 and embodiment 2, plant height, the list of soybean are significantly promoted
Strain English number and single-strain grain number Isoquant constituent element.
2 measurement data of table shows that there were significant differences with blank control is not coated for Adult plant soybean plant height, single-strain legumen number and sky
White control is compared to there were significant differences；Single plant English grain number is compareed also above blank control and commodity seed coat agent.Illustrate implementation of the present invention
The biological seed coating agent that example 1 and embodiment 2 provide is not only able to inhibit the generation of soybean root disease well, but also can promote
Into the growth of soybean and the increase of 100-grain weight, to reach volume increase purpose.
In addition, 2012 carry out field in Helen experiment station of Heilongjiang Province and Heihe experiment station not to be coated for blank control
Between cell sowing test, specific experiment data are as shown in table 3 below.
The variation of 3 soybean biological seed coating agent processed soybeans root rot of table
Note：Indicate no preventive effect
Biological seed coating agent provided by the invention is in Helen experiment station to the preventive effect of root rot and soybean cyst nematode
Compared with blank control, embodiment 1 is respectively 46.15% He to root rot and soybean cyst nematode inhibiting rate
70.72%, embodiment 2 is respectively 58.9% and 82.3% to root rot and soybean cyst nematode inhibiting rate, is all reached
The difference pole level of signifiance.And biological seed coating agent provided by the invention can significantly improve yield, the biological seed coating that embodiment 1 provides
22.67% and 25.72% has been respectively increased in Helen experiment station and Heihe experiment station in agent per mu yield compared with the control, and embodiment 2 carries
42.8% and 44.6% has been respectively increased in Helen experiment station and Heihe experiment station in the biological seed coating agent per mu yield of confession compared with the control.
Preferably prevent the above test results show that this biological seed coating agent has soybean cyst nematode and root rot
Effect, and soybean yields can be significantly improved, show good development prospect.
Embodiment described above is only to absolutely prove that preferred embodiment that is of the invention and being lifted, protection domain are unlimited
In this.Those skilled in the art on the basis of the present invention made by equivalent substitute or transformation, the present invention protection
Within the scope of, protection scope of the present invention is subject to claims.
Claims (9)
1. a kind of purposes of biological seed coating agent in controlling soybean root disease, which is characterized in that the soybean root disease packet
Include soybean cyst nematode and root rot；
The biological seed coating agent includes following component, and each component is prepared by mass percentage：
0.02%~0.15% CuSO4, 0.05%~0.26% (NH4)6Mo7O24·4H2O, 0.01%~0.05%
H3BO3, 0.1%~0.5% MnSO4, 0.04%~0.25% FeSO4, 1.5%~6% KH2PO4, 0.02%~1%
K2SO4, 2%~8% dispersant, 2%~5% film forming agent, 0.1%~1.2% warning coloration, 0.95%~2.65%
Binder, remaining is with killing the active biocontrol bacterial strain zymotic fluid of line, and the biocontrol bacterial strain zymotic fluid is simple bacillus
Zymotic fluid.
2. purposes according to claim 1, which is characterized in that including following component, each component is prepared by mass percentage：
0.04% CuSO4, 0.08% (NH4)6Mo7O24·4H2O, 0.02% H3BO3, 0.2% MnSO4, 0.22%
FeSO4, 5% KH2PO4, 0.05% K2SO4, 6% dispersant, 4% film forming agent, 1% warning coloration, 2.55% it is viscous
Agent is tied, remaining is with killing the active biocontrol bacterial strain zymotic fluid of line.
3. purposes according to claim 1, which is characterized in that the simple bacillus detaches from soybean root system soil
It obtains.
4. purposes according to claim 1, which is characterized in that the biocontrol bacterial strain zymotic fluid is to pass through bacterial strain to be seeded
After test tube strains culture, then through expanding made from fermented and cultured, specific preparation method is as follows：
It is respectively 0.2%~0.4% beef extract by weight percent, 0.4%~0.6% peptone, 0.1%~
0.2% yeast extract, 0.8%~1.0% sucrose, 1.2%~1.8% agar, remaining is distilled water, after being uniformly mixed
It is 7 to adjust pH, and simple Bacillus strain to be seeded is inoculated on the slant medium, sets by bevel culture medium
In 25 DEG C of constant temperature incubations 2 days, test tube strains are obtained；
It is respectively 0.2%~0.4% beef extract by weight percent, 1%~1.2% peptone, 0.5%~1%
Yeast extract, 1%-1.5% sucrose and excess water mixing adjust after pH is 6.8~7 and liquid fermentation medium are made later；
The test tube strains are inoculated into the liquid fermentation medium, at 25 DEG C~28 DEG C, with rotating speed 150rmin-1
~200rmin-1In shaking table shaken cultivation for 24 hours~72h, obtain the biocontrol bacterial strain zymotic fluid.
5. purposes according to claim 1, which is characterized in that the dispersant is calcium lignosulfonate；The film forming agent
For methylcellulose；The warning coloration is peacock green.
6. purposes according to claim 5, which is characterized in that the binder is that mass percent is 1.5-20: 100
The mixture of xanthans and converted starch composition.
7. purposes according to claim 1, which is characterized in that the preparation method of the biological seed coating agent is as follows：
It is respectively 0.02%~0.15% CuSO by weight percent4, 0.05%~0.26% (NH4)6Mo7O24·4H2O,
0.01%~0.05% H3BO3, 0.1%~0.5% MnSO4, 0.04%~0.25% FeSO4, 1.5%~6%
KH2PO4With 0.02%~1.0% K2SO4Attrition grinding after mixing, adds 2%~8% dispersant, 2%~5% at
Film, 0.1%~1.2% warning coloration, 0.95%~2.65% binder, remaining is with killing the active biocontrol bacterial strain of line
Zymotic fluid, said components stir into uniform viscous fluid, and oscillation is uniformly mixed, and prepares the biological seed coating agent.
8. purposes according to claim 7, which is characterized in that
The CuSO that the quality accounting of each component is 0.04%4, 0.08% (NH4)6Mo7O24·4H2O, 0.02% H3BO3,
0.2% MnSO4, 0.22% FeSO4, 5% KH2PO4With 0.05% K2SO4Attrition grinding after mixing, adds 6%
Dispersant, 4% film forming agent, 1% warning coloration, 2.55% binder, remaining is with killing line active biocontrol bacterial strain hair
Zymotic fluid.
9. purposes according to claim 1 or 2, which is characterized in that for coating soya seeds to be sowed, in use,
The biological seed coating agent that mass percent is 1-5: 70 is mixed with soya seeds, biological seed coating agent is made to be uniformly wrapped on soya seeds
Surface can be sowed after drying.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201511009463.5A CN105494447B (en) | 2015-12-23 | 2015-12-23 | Biological seed coating agent and its preparation method and application for controlling soybean root disease |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201511009463.5A CN105494447B (en) | 2015-12-23 | 2015-12-23 | Biological seed coating agent and its preparation method and application for controlling soybean root disease |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105494447A CN105494447A (en) | 2016-04-20 |
| CN105494447B true CN105494447B (en) | 2018-11-02 |
Family
ID=55703060
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201511009463.5A Active CN105494447B (en) | 2015-12-23 | 2015-12-23 | Biological seed coating agent and its preparation method and application for controlling soybean root disease |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105494447B (en) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106986719A (en) * | 2016-10-26 | 2017-07-28 | 沈阳农业大学 | And anti-root rot and seed coat agent, the preparation method of soybean cyst nematode |
| CN106818188A (en) * | 2017-03-03 | 2017-06-13 | 河南科技学院 | A kind of good quality and high output abridged edition protects excellent wheat cultivating method |
| NL2020261B1 (en) * | 2018-01-10 | 2019-07-15 | Rijk Zwaan Zaadteelt En Zaadhandel Bv | Method for treating plant seed |
| CN109923975A (en) * | 2019-03-21 | 2019-06-25 | 陆福 | A kind of implantation methods of semen sojae atricolor kind |
| CN110218665B (en) * | 2019-05-13 | 2021-12-14 | 沈阳农业大学 | Bacillus with corn leaf blight resistance inducing effect and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102308853A (en) * | 2011-03-02 | 2012-01-11 | 沈阳农业大学 | The biological seed coating agent of control soybean SCN |
| CN102925387A (en) * | 2012-10-26 | 2013-02-13 | 沈阳农业大学 | Bacillus simplex for inducing soybean to generate soybean cyst nematode resistance and application |
-
2015
- 2015-12-23 CN CN201511009463.5A patent/CN105494447B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102308853A (en) * | 2011-03-02 | 2012-01-11 | 沈阳农业大学 | The biological seed coating agent of control soybean SCN |
| CN102925387A (en) * | 2012-10-26 | 2013-02-13 | 沈阳农业大学 | Bacillus simplex for inducing soybean to generate soybean cyst nematode resistance and application |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105494447A (en) | 2016-04-20 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105494447B (en) | Biological seed coating agent and its preparation method and application for controlling soybean root disease | |
| CN103642714B (en) | For the production of the engineering strain and uses thereof of azophenlyene-1-methane amide | |
| US20220369648A1 (en) | Endophytic falciphora oryzae fo-r20 and its application | |
| CN108277177B (en) | Streptomyces microflavus solid fermentation medium, preparation method and fermentation method thereof, fermentation product, biocontrol product and application | |
| CN110627537A (en) | Multifunctional microbial fertilizer and preparation method thereof | |
| CN108676755A (en) | A kind of microbial liquid fertilizer and its preparation method and application containing bacillus | |
| CN112970780A (en) | Special microbial agent for ginseng and method for reducing incidence rate of ginseng rust rot | |
| CN105779360B (en) | A kind of bacillus subtilis and its application | |
| CN102851245A (en) | Tobacco endophyte strain inhibiting tobacco bacterial wilt, and microbial agent thereof | |
| CN109055274B (en) | Caragana rhizobium and fermentation culture method and application thereof | |
| CN102648714B (en) | Method for preparing biopesticide through liquid fermentation | |
| CN109749940B (en) | Preparation method of fungal inoculant, product and application thereof | |
| CN104195076A (en) | Bacillus methylotrophicus Sanju-04 and application thereof | |
| CN103667083B (en) | A kind of mould, its cultural method of top spore and the application in preparation original spore powder of meloidogyne pesticide thereof | |
| CN108531404B (en) | Culture method of Fusarium proliferatum and application thereof | |
| CN115197853B (en) | Endophyte Epicoccum thailandicum LF-28 strain and application thereof | |
| CN101818124B (en) | Stenotrophomonas capable of improving herbicide resistance in soybeans, method for preparing same and application thereof | |
| WO2023049961A1 (en) | Zopfiella fungal strains for improving plant productivity | |
| CN109207393A (en) | One bacillus amyloliquefaciens and its application in prevention and treatment celery root rot | |
| CN104193549A (en) | Biological additive for promoting alfalfa green manure to increase crop yield | |
| CN103937729A (en) | Method for improving salt resistance of tall fescue by virtue of air-dried sludge microbial agent | |
| CN106967615A (en) | One plant of gibberella and its application | |
| Narayanasamy et al. | Impact of Moisture Stress and Bacillus altitudinis FD48 on Physiological Modulation and Seed Germination in Rice (Oryza sativa L.). | |
| CN106929444B (en) | Bacillus and application thereof | |
| CN117660190B (en) | Trametes salina CGM001 and application thereof in improving salt tolerance of plants |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |