CN105380732B - Bone renovating material with more-dimensional channels structure - Google Patents
Bone renovating material with more-dimensional channels structure Download PDFInfo
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- CN105380732B CN105380732B CN201510937064.9A CN201510937064A CN105380732B CN 105380732 B CN105380732 B CN 105380732B CN 201510937064 A CN201510937064 A CN 201510937064A CN 105380732 B CN105380732 B CN 105380732B
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- Prior art keywords
- boss
- bone renovating
- renovating material
- hole
- bone
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Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
- A61F2/00—Filters implantable into blood vessels; Prostheses, i.e. artificial substitutes or replacements for parts of the body; Appliances for connecting them with the body; Devices providing patency to, or preventing collapsing of, tubular structures of the body, e.g. stents
- A61F2/02—Prostheses implantable into the body
- A61F2/28—Bones
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/40—Composite materials, i.e. containing one material dispersed in a matrix of the same or different material
- A61L27/42—Composite materials, i.e. containing one material dispersed in a matrix of the same or different material having an inorganic matrix
- A61L27/425—Composite materials, i.e. containing one material dispersed in a matrix of the same or different material having an inorganic matrix of phosphorus containing material, e.g. apatite
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/40—Composite materials, i.e. containing one material dispersed in a matrix of the same or different material
- A61L27/42—Composite materials, i.e. containing one material dispersed in a matrix of the same or different material having an inorganic matrix
- A61L27/427—Composite materials, i.e. containing one material dispersed in a matrix of the same or different material having an inorganic matrix of other specific inorganic materials not covered by A61L27/422 or A61L27/425
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/54—Biologically active materials, e.g. therapeutic substances
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/56—Porous materials, e.g. foams or sponges
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
- A61F2/00—Filters implantable into blood vessels; Prostheses, i.e. artificial substitutes or replacements for parts of the body; Appliances for connecting them with the body; Devices providing patency to, or preventing collapsing of, tubular structures of the body, e.g. stents
- A61F2/02—Prostheses implantable into the body
- A61F2/30—Joints
- A61F2002/30001—Additional features of subject-matter classified in A61F2/28, A61F2/30 and subgroups thereof
- A61F2002/30003—Material related properties of the prosthesis or of a coating on the prosthesis
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
- A61F2/00—Filters implantable into blood vessels; Prostheses, i.e. artificial substitutes or replacements for parts of the body; Appliances for connecting them with the body; Devices providing patency to, or preventing collapsing of, tubular structures of the body, e.g. stents
- A61F2/02—Prostheses implantable into the body
- A61F2/30—Joints
- A61F2002/30001—Additional features of subject-matter classified in A61F2/28, A61F2/30 and subgroups thereof
- A61F2002/30108—Shapes
- A61F2002/30199—Three-dimensional shapes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
- A61F2/00—Filters implantable into blood vessels; Prostheses, i.e. artificial substitutes or replacements for parts of the body; Appliances for connecting them with the body; Devices providing patency to, or preventing collapsing of, tubular structures of the body, e.g. stents
- A61F2/02—Prostheses implantable into the body
- A61F2/30—Joints
- A61F2002/30001—Additional features of subject-matter classified in A61F2/28, A61F2/30 and subgroups thereof
- A61F2002/30316—The prosthesis having different structural features at different locations within the same prosthesis; Connections between prosthetic parts; Special structural features of bone or joint prostheses not otherwise provided for
- A61F2002/30329—Connections or couplings between prosthetic parts, e.g. between modular parts; Connecting elements
- A61F2002/30383—Connections or couplings between prosthetic parts, e.g. between modular parts; Connecting elements made by laterally inserting a protrusion, e.g. a rib into a complementarily-shaped groove
- A61F2002/30385—Connections or couplings between prosthetic parts, e.g. between modular parts; Connecting elements made by laterally inserting a protrusion, e.g. a rib into a complementarily-shaped groove the rib and groove having non-parallel, e.g. conically-tapered, cooperating sides, e.g. having a trapezoidal front cross-section
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
- A61L2300/30—Compounds of undetermined constitution extracted from natural sources, e.g. Aloe Vera
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/412—Tissue-regenerating or healing or proliferative agents
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- A—HUMAN NECESSITIES
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- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2430/00—Materials or treatment for tissue regeneration
- A61L2430/02—Materials or treatment for tissue regeneration for reconstruction of bones; weight-bearing implants
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Veterinary Medicine (AREA)
- Oral & Maxillofacial Surgery (AREA)
- Transplantation (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Medicinal Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Epidemiology (AREA)
- Dermatology (AREA)
- Biomedical Technology (AREA)
- Inorganic Chemistry (AREA)
- Composite Materials (AREA)
- Materials Engineering (AREA)
- Dispersion Chemistry (AREA)
- Molecular Biology (AREA)
- Orthopedic Medicine & Surgery (AREA)
- Cardiology (AREA)
- Heart & Thoracic Surgery (AREA)
- Vascular Medicine (AREA)
- Prostheses (AREA)
- Materials For Medical Uses (AREA)
Abstract
The present invention discloses the bone renovating material with more-dimensional channels structure, the basal body structure with stratiform；One surface of described matrix structure is provided with several columns boss I, and another surface is provided with several columns boss II；The boss I is spaced with boss II；Groove is constituted between boss II between adjacent boss I, adjacent；Several through holes are provided with perpendicular to the surface of the boss I.The bone renovating material that the present invention is provided, by the built-up cubic network passage of the through hole and groove of basal body structure, wherein it is filled with collagenous fibres, cell adherence growth and blood vessel and neural is conducive to grow into, the transmission of nutriment and the exclusion of cell metabolite are also allowed for, while cell can be made to be uniformly distributed in material internal is conducive to organizing the formation of for internal stent uniformity；Form firm biological chimeric between the structural material energy and freshman bone tissue, good structural stability can be also maintained in degradation process；Mechanical strength is high, can well realize the mechanics transmission between Cranial defect position and normal bone tissues.
Description
Technical field
The invention belongs to field of biomedical materials, it is related to the bone renovating material of clinical practice, more particularly to intensity
It is enhanced with multidimensional it is porous and with slow release treatment effect bone renovating material.
Background technology
Cranial defect is very common disease in clinic, but bone defect healing is still the problem faced in Orthopedic Clinical,
Although scientific worker has done substantial amounts of work in prepared by the research of clinical bone renovating material for many years, make much progress,
But should so there are problems.
The sclerotin of natural bone is according to the spread pattern of hone lamella and the density of space structure is different is divided into compact bone and spongy bone,
Fine and close hard anti-pressure anti-torsion Qu Nengli is strong for compact bone, is distributed in the surface of bone, and compact bone has orderly anisotropic structure, main
It is satisfied with its mechanical performance needs.Bone tissue is mainly hydroxyapatite along collagenous fibres major axis mineralising with many
The composite of pore structure, its structure and mechanical property performance anisotropy.Exactly this anisotropic orientation knot of natural bone
Structure, thus with optimal mechanical advantage and physiologic function advantage.
It is histiocytic to grow into bone tissue reparation, the generation of new blood vessel, the exchange of nutrition and metabolism thing and support
The degraded of material requires that repair materials have the porosity and connected ratio of height.The loose structure system of bone renovating material is new
Bone Ingrowth and wound repair necessary requirement, do not have these loose structures, the material being implanted into can not complete cell creep and into
Bone alternative Process.Porosity is too low, and surface area is too small, is unfavorable for growing into for tissue, is also unfavorable for support degraded in vivo.Cause
Under the premise of the stability and mechanics for ensureing supporting structure, repair materials should have porosity and connected ratio high for this.Hole it is big
The small activity that can yet influence skeletonization, too small aperture does not have growth of enough spaces for cell, influence cell secretion matrix and
The infiltration of nutriment.Research shows that 15~50 μm of aperture can induce growing into for fiber tubing, 50~150 μm of aperture
The generation of osteoid tissue can be stimulated, and 150~500 μm of aperture can directly induce the generation of mineralising bone.Too big aperture meeting
Influence the mechanical property of material.Hole is not communicated with making to be connected with each other between new bone, and lacks continuity and conformability.Together
When cell can be made to be uniformly distributed in material internal be conducive to organizing the formation of for internal stent uniformity.
Although patent CN1325734A, CN101297980A, CN1325734A, CN1647826A, CN103830775A
With the bone renovating material that collagen and hydroxyapatite are prepared as main material, but it is even poisonous also to add part organic polymer
The organic solvent of property.These materials exist universal shortcoming be：(1) collagenous fiber material or mineralized collagen material are irregular
It is irregular with orientation each to adhesion is weak between material molecule, does not have enough mechanical strengths, and katabolism is fast, it is impossible to full
Sufficient skeletonization needs；(2) aperture of material is smaller, and connected ratio is low, and common problem is freshman bone tissue only in the outer of support
Edge is formed, and makes cell be difficult to penetrate into internal stent, hampers the exchange of internal stent body fluid, and ultimately result in timbering material
The apoptosis of portion's cell, can make be connected with each other between new bone, and lack continuity and conformability, influence the weight of big defect area
Build.(3) there is noxious material residual, be unfavorable for clinical practice.
Bone renovating material of the present invention is formed by stacking by multilayer material, and every layer of matrix material all has certain orientation,
The porous three-dimensional structure of the orientation material can well realize the mechanics transmission between Cranial defect position and normal bone tissues.Planting
Defect initial stage to the marrow, bone renovating material can provide certain mechanical support for defect.This network can be protected
Card hole connectivity, is conducive to that cell adherence grows and blood vessel and nerve grow into, and also allows for the transmission of nutriment and thin
The exclusion of born of the same parents' metabolin.
Form firm biological chimeric when support starts degraded, between the structure energy and freshman bone tissue, also can be in drop
Good structural stability is maintained in solution preocess.As osteocyte and blood vessel grow into neural, new bone gradually recovers itself
Biomechanical property, while material is also gradually degraded, and catabolite no side effectses, and with the nature of body
Physiological metabolism and excrete, completed reparation and the reconstruction of bone tissue.
The content of the invention
For the mechanical property for solving artificial bone renovating material is low, the defect that aperture is small and hole Penetration ration is poor, the present invention is provided
Artificial bone renovating material with more-dimensional channels structure, the present invention is realized using following scheme：
Bone renovating material with more-dimensional channels structure, the basal body structure with stratiform；One table of described matrix structure
Face is provided with several columns boss I, and another surface is provided with several columns boss II；The boss I is spaced with boss II；Adjacent is convex
Groove is constituted between boss II between platform I, adjacent；Several through holes are provided with perpendicular to the surface of the boss I.
Passage of the present invention includes main channel and non-main channel：
Main channel is made up of groove, through hole；
Non- main channel refers to the passage that the hole that the basal body structure in addition to groove, through hole has is constituted.
Used as preferred technical scheme, basal body structure stacking of the material described at least two-layer is constituted；Adjacent two layers
The groove orientation of basal body structure is substantially vertical；Successively the through hole of basal body structure is correspondingly connected, and is easy to form vertical passage.
Used as preferred technical scheme, the hole wall of the through hole closes on groove has a crack, the through hole through crack with
Adjacent groove connection.
Used as preferred technical scheme, the thickness of described matrix structure (after isostatic cool pressing contracting) is 600~1500 μm；It is described
A diameter of 180~590 μm of through hole；The width of the groove is 180~590 μm, and depth is 180~590 μm.
Used as preferred technical scheme, in each column boss I, the spacing of through hole is 180~590 μm.
Used as preferred technical scheme, the orientation of the through hole and the angle of described matrix structure are 75~90 degree.
Used as preferred technical scheme, described matrix structure contains mineralized collagen, collagen, ceramic powder and golden yellow Portugal
Grape coccus fermentating liquid filtrate；The mineralized collagen, collagen, the weight ratio of ceramic powder are 10~13:1.5~4.5:1~3；
The addition of the S. aureus fermentation liquid filtrate is with staphylococcus aureus coagulase activity 1600 in basal body structure
~3200 μ g/g are meter.
As preferred technical scheme, the ceramic powder include following weight than silica 1~5, magnesia 1~5,
Calcirm-fluoride 1~5.
Collagenous fibres and staphylococcus aureus hair are filled as preferred technical scheme, in the groove and/or through hole
Zymotic fluid filtrate；The collagenous fibres are 5.0~15 with the weight ratio of S. aureus fermentation liquid filtrate:0.12~0.4.
Used as preferred technical scheme, the three-point bending strength of the bone renovating material is more than 40.0Mpa, and compressive strength is big
In 51.8Mpa.
The preparation method of the bone renovating material with more-dimensional channels structure, the bone renovating material is by least two stratiforms
Basal body structure stacking is constituted；Described matrix structure has through hole and groove；
Step one：Prepare matrix material
Mineralized collagen is mixed with ceramic powder, is stirred, adjust pH value 7.2~8.0, taking precipitate is centrifuged；Add golden yellow
Staphylococcal fermentation liquid filtrate and collagen, stirring, regulation pH value is 7.2~7.4, and vacuum outgas obtains matrix material；Mineralising
Collagen, collagen, ceramic powder and S. aureus fermentation liquid filtrate weight ratio are 10~13:1.5~5:1~3:0.5
~1；Regulation pH value uses phosphoric acid, NaOH, sodium carbonate or sodium acid carbonate.
Step 2：Prefabricated matrix (preparing the basal body structure of the stratiform before compression)
During matrix material prepared by step one is along same direction press-in die, prefabricated matrix thickness is set to be 8~12mm；
Recess width is 2~4mm, and depth of groove is 2~4mm；Boss I is 2~4mm with the width of boss II；Through-hole diameter is 3~5mm,
Through hole spacing is 2~4mm in each column boss I.Then under conditions of 30~40 DEG C, relative humidity are for more than 90%, ageing 8~
12h, obtains prefabricated matrix.
Step 3：Prepare filler
Collagenous fibres are mixed with S. aureus fermentation liquid filtrate, is stirred, vacuum outgas；Collagenous fibres with it is golden yellow
The weight ratio of color Staphylococcal fermentation liquid filtrate is 5.0~15:0.12~0.4.
Step 4：Filling and stacking
The filler that in the groove and through hole of the upper and lower surface of the prefabricated matrix prepared to step 2 prepared by filling step three,
Then more than stacking two-layer, communicate inter-level vias during stacking, through hole and matrix material planar shaped are angled 75~90 degree；So
After be laminated more than prefabricated matrix at least two-layer, obtain layered product.
Step 5：Sizing
Layered product prepared by step 4 freezes shaping in moving into liquid nitrogen, and vacuum freeze drying is cold using 200~400MPa
Isostatic pressed contracts.
Step 6：Hot-working
105~125 DEG C of 8~12h of heat treatment, are obtained sample under nitrogen protective condition.
Stirring of the present invention, it is consistent to be stirred or consistent to stir counterclockwise for clockwise using stirring in the same direction.
Bone renovating material finished product generous size long prepared by the present invention can be selected according to clinic needs, preferably length and width
No more than 5.0cm, thickness is no more than 1.0cm, or the treatment for carrying out material profile, it is also possible to carry out fragmented treatment, so as to
For the filling of micropore.
The bone renovating material with more-dimensional channels structure that the present invention is provided is used for repair of fractured bones, bone defect healing or fills out
Fill, bone is orthopedic, middle Cranial defect is replaced or overhauled in bone grafting fusion or joint prosthesis.The fracture includes closed or open bone
Folding；The Cranial defect includes Cranial defect after congenital bone exception, neoplastic lesion excision；The bone grafting fusion includes spinal vertebral
Between, between vertebral plate phalanx fusion；The Cranial defect filling includes the Cranial defect filling of cavity section.
Advantages of the present invention：
The bone renovating material that the present invention is provided, by the built-up cubic network passage of the through hole and groove of basal body structure,
Collagenous fibres are wherein filled with, are conducive to cell adherence growth and blood vessel and neural to grow into, also allow for the biography of nutriment
The exclusion with cell metabolite is passed, while can make cell that the group for being conducive to internal stent uniformity is uniformly distributed in material internal
Knit to be formed；Form firm biological chimeric between the structural material energy and freshman bone tissue, also can remain good in degradation process
Good structural stability；Using the method prepare bone renovating material mechanical strength it is high, can realize well Cranial defect position with
Mechanics transmission between normal bone tissues.At the implantation Cranial defect position initial stage, bone renovating material can provide certain for defect
Mechanical support；Bone renovating material degradation time is long, and consistent with the generation of freshman bone tissue, beneficial to damaged bone tissue reparation.
The present invention carries out the treatment of up to 200~400MPa isostatic cool pressings using by sample, after basal body structure is through overcompression,
The distance between molecule diminishes, and the active force between Van der Waals force, hydrogen bond equimolecular is stronger, particularly across material after hot dehydrogenation crosslinking
The intensity of material is bigger；The aperture of collagenous fibres body is 130~450 μm in material；Micropore occupation rate is 60~90%.Realize true
The three-dimensional hole connectivity structure of positive meaning, and this aperture structure is adapted to growing into for cell, and cell can be made uniform in material internal
Organizing the formation of beneficial to internal stent uniformity is distributed with.
The component ratio of collagen and hydroxyapatite is basically identical with nature bone in material.The pottery that basal body structure is used
Porcelain powder is inorganic porous material, not only to the inducing action that is grown with of cell, is also had to the mechanical strength for improving bone renovating material
Positive role, it is to avoid material too fast degraded.
All contain the S. aureus fermentation liquid filtrate for promoting fracture to have Healing in matrix and filler.It is golden yellow
The main component of color Staphylococcal fermentation liquid filtrate is protein, polypeptide, 18 kinds of amino acid, free coagulases.Main pharmacological
Effect is to promote union, treats osteoporosis.Mainly intramuscular injection, effect are carried out in clinical practice in diseased region
Very well, but side effect is to cause the phenomenons such as local swelling sense, pain, heating, patient is allowed to produce repulsion.The handle of the invention
S. aureus fermentation liquid filtrate is added in bone renovating material, keeps S. aureus fermentation liquid filtrate in material
Middle sustained release, plays its unique pharmacological action, golden in collagenous fibres in advance with the infiltration and the degraded of material of tissue fluid
The pharmaceutical component of staphylococcus aureus fermentating liquid filtrate is first quickly discharged, and the pharmaceutical component in subsequent matrix material is with base
The decomposition of body material carries out follow-up sustained release, plays long-acting.
Brief description of the drawings
The width of accompanying drawing of the present invention 4,
Fig. 1 is bone renovating material structural representation of the present invention；
Fig. 2 is the scanning electron microscope (SEM) photograph that embodiment 4 prepares sample；
The non-main channel section scanning electron microscope image of Fig. 3 bone renovating materials, the visible bone renovating material from scanning figure
Structure be multi-C stereo, non-main channel section aperture be 30-180 μm, micropore occupation rate be 85%；
Pore-size distribution in Fig. 4 bone renovating materials main channel, it is seen that the aperture of collagenous fibres is mainly 130-450 in main channel
μm；
In figure, 1 basal body structure；2 boss I；3 boss II；4 grooves；5 through holes；6 cracks.
Specific embodiment
Following non-limiting examples can make one of ordinary skill in the art be more fully understood the present invention, but not with
Any mode limits the present invention.
Raw material of the present invention is to be capable of achieving invention effect using art technology versatile material, below for embodiment is preferred
Pretreatment of raw material scheme：
1st, mineralized collagen：Preparation method is referring to Masanori Kikuchi, Soichiro Itoh, ect.Self-
organization mechanism in a Bone-Like Hydroxyapatite/Collagen Nanocomposite
synthesized in vitro and Its Biological Raction in vivo.Biomaterials 22(2001)
1705-1711.Wherein Ca:P=1~2:1, hydroxyapatite:Collagen=4:0.8~1.2, the c-axis of hydroxyapatite and
Collagenous fibres length is parallel, and electrical conductivity is less than 20us/cm, water content 80% (g/g).
2nd, ceramic powder：To mix with PLA by weight silica 1~5, magnesia 1~5, calcirm-fluoride 1~5, into
Type, 200 DEG C of drying, then calcines 5h at 1100~1300 DEG C, and particle diameter is ground into after cooling for 80~300 μm, and aperture is 60~
210 μm, porosity is more than 80%, and partial hole is the cell structure for communicating.
3rd, S. aureus fermentation liquid filtrate：The golden yellow of commercially available (reaching biochemical Pharma Inc. purchased from Changchun profound scholar)
It is the milipore filter ultrafiltration of 20000 dalton that Staphylococcal fermentation liquid filtrate uses molecular cut off, and film top is divided into containing coagulase
Concentrate, then 40 DEG C of cryogenic vacuum concentrations of warp -0.098MPa prepare S. aureus fermentation liquid filtrate solidification enzymatic activity not
Less than the concentrate of 3200 μ g/ml.
4th, collagenous fibres：
A. ox heel string that purity is more than 99.5% and/or the I collagens of horse heel string are dissolved in concentration for 20mM acetic acid or phosphorus
In acid solution, collagen concentration is 2.0g/L, and to alkali lye is added dropwise in collagen solution, concentration is 70mM, and keeps 10mM Mg2+、20mM
K+、20mM HPO4 3+Ion, pH value 7.0.Collagenous fibres are obtained, 4h is aged, then in 4 DEG C of 6000rpm centrifugation 30min, collected
Collagenous fibres are precipitated, water content 85% (g/g).
The mould that embodiment 1-4 is used to suppress basal body structure is processed according to following structure：
Matrix has the basal body structure 1 of stratiform；One surface of described matrix structure 1 is provided with several columns boss I 2, another
Surface is provided with several columns boss II 3；The boss I 2 is spaced with boss II 3；Between adjacent boss I 2, adjacent boss
Groove 4 is constituted between II 3；Several through holes 5 are provided with perpendicular to the surface of the boss I 2.Matrix of the material as described at least two-layer
The stacking of structure 1 is constituted；The groove 4 of adjacent two layers basal body structure 1 is orientated substantially vertical；Successively the through hole 5 of basal body structure 1 is correspondingly
Connection.The hole wall of through hole 5 closes on has crack 6 at groove 4, the through hole 5 is connected through crack 6 with adjacent groove 4.
Embodiment 1
It is prepared by bone renovating material
Step one prepares matrix material
50g mineralized collagens material (water content is 80%) is weighed, ceramic powder 2g is added thereto to, it is suitable with the rotating speed of 100rpm
Hour hands are stirred, and pH value is adjusted to 7.0 with 1.0M phosphoric acid liquids and 1.0M sodium hydroxide lyes, then use 0.5M sodium carbonate liquors
To 7.2, electrical conductivity is 1.5ms/cm to regulation pH value.4 DEG C of centrifugation 30min of static 30min, 10000rpm remove supernatant.Xiang Chen
S. aureus fermentation liquid filtrate is added in shallow lake, makes solidification enzymatic activity for 1600 μ g/g, add 37.5g collagen materials
(purity of collagen be 40mg/g), continues same direction uniform stirring, and adjusts precipitation to make compound mineralization collagen matrices
PH value is 7.2, and electrical conductivity is 3.0ms/cm.More than 1.01 × 102Deaerated under Pa vacuum conditions, be further continued for using 1.0M phosphoric acid liquids
Electrical conductivity is set to be 7.0ms/cm with the regulation of 1.0M sodium hydroxide lyes.
The prefabricated matrix of step 2 (prepares the stratiform basal body structure before compression)
The matrix material of preparation is complied with into direction press-in die to same, makes the thickness of prefabricated matrix for 8mm, groove depth
It is 2mm to spend, and recess width is 2mm；Boss I and the width 2mm of boss II；Through-hole aperture 3mm, Fracture Width 1mm；Each column boss I
Middle through hole spacing is 2mm.Then at 30 DEG C, relative humidity be more than 90% under conditions of be aged 12h, obtain prefabricated matrix.
Step 3 prepares filler
Weighing collagenous fibres adds water to be diluted to 15mg/g, is added thereto to S. aureus fermentation liquid filtrate, makes
S. aureus fermentation liquid filtrate coagulation activity is 400 μ g/g, is sequentially stirred, 1.01 × 103It is true under Pa pressure
Sky degassing.
Step 4 filling, stacking
The filler that in the groove and through hole of the prefabricated matrix upper and lower surface prepared to step 2 prepared by filling step three；Fill out
The lateral opposite side levels of Shi Congyi are filled to spread into or be filled with；Then the three-dimensional prefabricated matrix of stacking is carried out, the matrix number of plies is 4 layers, stacking
When communicate inter-level vias, through hole and matrix material planar shaped are angled 75~90 degree, obtain layered product.
Step 5 is shaped
Layered product prepared by step 4 is according to freezing shaping, then vacuum in slowly moving into liquid nitrogen perpendicular to liquid level direction
Freeze-drying, when product moisture content is 10~15% (g/g), carries out the isostatic cool pressing contracting of 200MPa, is then further continued for true
It is less than 5% that sky is dried to moisture.
After compression, bone renovating material thickness is 4000 μm；Non- main channel section aperture is 30~180 μm, main channel section
Aperture is 130~450 μm.
Step 6 hot-working
Material is carried out into the lower 110 DEG C of crosslinkings 10h of high pure nitrogen protective condition, product is obtained.
Embodiment 2
Step one prepares matrix material
65g mineralized collagens material (water content is 80%) is weighed, ceramic powder 2g is added thereto to, it is suitable with the rotating speed of 100rpm
Hour hands stir, and then adjust pH value to 7.0 with 1.0M phosphoric acid liquids and 1.0M sodium hydroxide lyes, then use 0.5M sodium carbonate
Solution adjusts pH value to 7.2, and electrical conductivity is 1.5ms/cm.4 DEG C of centrifugation 30min of static 30min, 10000rpm remove supernatant.
To S. aureus fermentation liquid filtrate is added in precipitation, make solidification enzymatic activity for 3200 μ g/g, add 75g collagen materials
Material (purity of collagen be 40mg/g), continues same direction uniform stirring, and adjusts precipitation to make compound mineralization collagen matrices
PH value be 7.2, electrical conductivity is 3.0ms/cm.More than 1.01 × 102Deaerated under Pa vacuum conditions, be further continued for using 1.0M phosphoric acid
Liquid and the regulation of 1.0M sodium hydroxide lyes make electrical conductivity be 7.0ms/cm.
The prefabricated matrix of step 2 (prepares the basal body structure of the stratiform before compression)
Matrix material will be prepared and comply with direction press-in die to same, make the thickness of prefabricated matrix for 9mm, depth of groove
It is 4mm, recess width is 3mm；Boss I and the width 3mm of boss II；Through-hole aperture 4mm, Fracture Width 1mm；In each column boss I
Through hole spacing is 3mm.Then at 30 DEG C, relative humidity be more than 90% under conditions of be aged 12h, obtain prefabricated matrix.
Step 3 prepares filler
Weighing collagenous fibres adds water to be diluted to 10mg/g, is added thereto to S. aureus fermentation liquid filtrate, makes
S. aureus fermentation liquid filtrate coagulation activity is 400 μ g/ml, is sequentially stirred, 1.01 × 103It is true under Pa pressure
Sky degassing.
Step 4 filling, stacking
To the upper and lower surface of the prefabricated matrix of step 2 preparation, groove and through hole filler from a lateral opposite side level
Spread into or be filled with；Then the three-dimensional prefabricated matrix of stacking is carried out, the matrix material number of plies is 4 layers, communicates inter-level vias during stacking, is led to
Hole and matrix material planar shaped are angled 75~90 degree, obtain layered product.
Step 5 is shaped
Layered product prepared by step 4 is according to freezing shaping, then vacuum in slowly moving into liquid nitrogen perpendicular to liquid level direction
Freeze-drying, when product moisture content is 10~15% (g/g), carries out the isostatic cool pressing contracting of 200MPa, is then further continued for true
It is less than 5% that sky is dried to moisture.
After compression, bone renovating material thickness is 4000 μm；Non- main channel section aperture is 30~180 μm, main channel section
Aperture is 130~450 μm.
Step 6 hot-working
Material is carried out into the lower 105 DEG C of crosslinkings 12h of high pure nitrogen protective condition, product is obtained.
Embodiment 3
Step one prepares matrix material
50g mineralized collagens material (water content is 80%) is weighed, ceramic powder 1g is added thereto to, with turning for 100rpm
Speed stirs clockwise, then adjusts pH value to 7.0 with 1.0M phosphoric acid liquids and 1.0M sodium hydroxide lyes, then uses 0.5M carbon
Acid sodium solution adjusts pH value to 7.2, and electrical conductivity is 1.5ms/cm.Static 30min, 10000rpm4 DEG C of centrifugation 30min removes supernatant
Liquid.To S. aureus fermentation liquid filtrate is added in precipitation, make solidification enzymatic activity for 1600 μ g/g, add 75g collagen eggs
White material (purity of collagen is 40mg/g), continues same direction uniform stirring, and adjusts precipitation to make compound mineralization collagen
The pH value of matrix is 7.2, and electrical conductivity is 3.0ms/cm.More than 1.01 × 102Deaerated under Pa vacuum conditions, be further continued for using 1.0M
Phosphoric acid liquid and the regulation of 1.0M sodium hydroxide lyes make electrical conductivity be 8.0ms/cm.
The prefabricated matrix of step 2 (prepares the basal body structure of the stratiform before compression)
The matrix material of preparation is complied with into direction press-in die to same, makes prefabricated matrix thickness for 8mm, depth of groove
It is 2.5mm, recess width is 2.5mm；Boss I and the width 2.5mm of boss II；Through-hole aperture 3.5mm, Fracture Width 1mm；Each column
Through hole spacing is 2.5mm in boss I.Then at 30 DEG C, relative humidity be more than 90% under conditions of be aged 12h, obtain prefabricated base
Body.
Step 3 prepares filler
Weighing collagenous fibres adds water to be diluted to 8mg/g, is added thereto to S. aureus fermentation liquid filtrate, makes gold
Staphylococcus aureus fermentating liquid filtrate coagulation activity is 400ug/g, is sequentially stirred, 1.01 × 103Pa vacuum under pressure
Degassing.
Step 4 filling, stacking
The groove and through hole filler of the upper and lower surface of the prefabricated matrix prepared to step 2 are from a lateral opposite side water
Tile into or be filled with；Then the three-dimensional prefabricated matrix of stacking is carried out；The matrix material number of plies is 4 layers, communicates inter-level vias during stacking,
Through hole and matrix material planar shaped are angled 75~90 degree, obtain layered product.
Step 5 is shaped
Layered product prepared by step 4 is according to freezing shaping, then vacuum in slowly moving into liquid nitrogen perpendicular to liquid level direction
Freeze-drying, when product moisture content is 10~15% (g/g), carries out the isostatic cool pressing contracting of 200MPa, is then further continued for true
It is less than 5% that sky is dried to moisture.
After compression, bone renovating material thickness is 4000 μm；Non- main channel section aperture is 30~180 μm, main channel section
Aperture is 130~450 μm.
Step 6 hot-working
Material is carried out into the lower 110 DEG C of crosslinkings 10h of high pure nitrogen protective condition, product is obtained.
Embodiment 4
Step one prepares matrix material
65g mineralized collagens material (water content is 80%) is weighed, ceramic powder 3g is added thereto to, it is suitable with the rotating speed of 100rpm
Hour hands stir, and then adjust pH value to 7.0 with 1.0M phosphoric acid liquids and 1.0M sodium hydroxide lyes, then use 0.5M sodium carbonate
Solution adjusts pH value to 7.2, and electrical conductivity is 2.5ms/cm.4 DEG C of centrifugation 30min of static 30min, 10000rpm remove supernatant.
To S. aureus fermentation liquid filtrate is added in precipitation, make solidification enzymatic activity for 3200 μ g/g, add 112.5g collagen eggs
White material (purity of collagen is 40mg/g), continues same direction uniform stirring, and adjusts precipitation to make compound mineralization collagen
The pH value of matrix is 7.2, and electrical conductivity is 3.0ms/cm.More than 1.01 × 102Deaerated under Pa vacuum conditions, be further continued for using 1.0M
Phosphoric acid liquid and the regulation of 1.0M sodium hydroxide lyes make electrical conductivity be 10.0ms/cm.
The prefabricated matrix of step 2 (prepares the basal body structure of the stratiform before compression)
The matrix material of preparation is complied with into direction press-in die to same, makes prefabricated matrix thickness for 9mm, depth of groove
It is 4mm, recess width is 4mm；Boss I and the width 4mm of boss II；Through-hole aperture 5mm, Fracture Width 1mm；In each column boss I
Pitch of holes is 4mm.Then at 30 DEG C, relative humidity be more than 90% under conditions of be aged 12h, obtain prefabricated matrix.
Step 3 prepares filler
Weighing collagenous fibres adds water to be diluted to 5mg/g, is added thereto to S. aureus fermentation liquid filtrate, makes gold
Staphylococcus aureus fermentating liquid filtrate coagulation activity is 400ug/g, is sequentially stirred, 1.01 × 103Pa vacuum under pressure
Degassing.
Step 4 filling, stacking
The groove and through hole filler of the upper and lower surface of the prefabricated matrix prepared to step 2 are from a lateral opposite side water
Tile into or be filled with, then carry out the three-dimensional prefabricated matrix of stacking；The matrix material number of plies is 4 layers, and upper lower opening is corresponded during lamination,
Obtain layered product.
Step 5 is shaped
Layered product prepared by step 4 is according to freezing shaping, then vacuum in slowly moving into liquid nitrogen perpendicular to liquid level direction
Freeze-drying, when product moisture content is 10~15% (g/g), carries out the isostatic cool pressing contracting of 200MPa, is then further continued for true
It is less than 5% that sky is dried to moisture.
After compression, bone renovating material thickness is 4200 μm；Non- main channel section aperture is 30~180 μm, main channel section
Aperture is 130~450 μm.
Step 6 hot-working
Material is carried out into the lower 125 DEG C of crosslinkings 8h of high pure nitrogen protective condition, sample is obtained.
Result is detected
1 bending strength is detected
Bone renovating material sample is prepared according to embodiment 1, the sample number of plies is 4 layers, and after 200Mpa compressions, a length of 20mm is wide
10mm, thickness is 4.0mm.Sample is carried out into three-point bending detection with the Apparatus for Bending at low-temp of Instron (instron) 5569, by span
15mm is set to, loading velocity is 3mm/min, after sample is loaded, load and displacement is returned to zero, load is set to 1000N.It is real
Test speed and be set to 3mm/min, click on the operation button on computer interface, carry out bend test, testing result is as follows：
The bending strength testing result of the sample of table one
2 compressive strengths are detected
Bone renovating material sample is prepared according to embodiment 4, the sample number of plies is 4 layers, and after 200Mpa compressions, a length of 20mm is wide
10mm, thickness is 4.2mm, and sample is carried out into intensity detection experiment, compression strength with Instron (instron) 5500R testing machines
The maximum pressure that unit area is born during by sample deformation 1mm, compression speed is 0.3mm/min, and testing result is as follows：
The compressive strength testing result of the sample of table two
3 product structures are detected
Sample is prepared according to embodiment 4, the sample number of plies is 4 layers, after 200Mpa compressions, a length of 20mm, 10mm wide, thickness is
4.2mm.By sample metal spraying, with SEM EVO18 (SEM)) carry out micro-structural scanning, non-main channel section scanning figure
Spectrum is shown in Fig. 3, and bone renovating material main channel section aperture ratio is shown in Fig. 4.
Claims (10)
1. there is the bone renovating material of more-dimensional channels structure, the basal body structure (1) with stratiform, the one of described matrix structure (1)
Individual surface is provided with several columns boss I (2), and another surface is provided with several columns boss II (3), between adjacent boss I (2), adjacent
Boss II (3) between constitute groove (4), it is characterised in that：
The boss I (2) is spaced with boss II (3)；
Several through holes (5) are provided with perpendicular to the surface of the boss I (2).
2. the bone renovating material with more-dimensional channels structure according to claim 1, it is characterised in that：
Basal body structure (1) stacking of the material described at least two-layer is constituted；
Groove (4) orientation of adjacent two layers basal body structure (1) is substantially vertical；
Successively the through hole (5) of basal body structure (1) is correspondingly connected.
3. the bone renovating material with more-dimensional channels structure according to claim 1, it is characterised in that：
The hole wall of the through hole (5) closes on groove (4) place has a crack (6), and the through hole (5) is recessed with adjacent through crack (6)
Groove (4) is connected.
4. the bone renovating material with more-dimensional channels structure according to claim 1, it is characterised in that：
The thickness of described matrix structure (1) is 600~1500 μm；
A diameter of 180~590 μm of the through hole (5)；
The width of the groove (4) is 180~590 μm, and depth is 180~590 μm.
5. the bone renovating material with more-dimensional channels structure according to claim 1, it is characterised in that：
In each column boss I (2), the spacing of through hole is 180~590 μm.
6. the bone renovating material with more-dimensional channels structure according to claim 1, it is characterised in that：
The orientation of the through hole (5) and the angle of described matrix structure (1) are 75~90 degree.
7. the bone renovating material with more-dimensional channels structure according to claim 1, it is characterised in that：
Described matrix structure contains mineralized collagen, collagen, ceramic powder and S. aureus fermentation liquid filtrate；The ore deposit
It is 10~13 to change collagen, collagen, the weight ratio of ceramic powder:1.5~4.5:1~3；The S. aureus fermentation liquid
The addition of filtrate solidifies the μ g/g of enzymatic activity 1600~3200 to count with S. aureus fermentation liquid filtrate in basal body structure.
8. the bone renovating material with more-dimensional channels structure according to claim 7, it is characterised in that：
The ceramic powder include following weight than silica 1~5, magnesia 1~5, calcirm-fluoride 1~5.
9. the bone renovating material with more-dimensional channels structure according to claim 1 or 2 or 3, it is characterised in that：
Filling collagenous fibres and S. aureus fermentation liquid filtrate in the groove (4) and/or through hole (5)；
The collagenous fibres are 5.0~15 with the weight ratio of S. aureus fermentation liquid filtrate:0.12~0.4.
10. the bone renovating material with more-dimensional channels structure according to claim 1, it is characterised in that：
The three-point bending strength of the bone renovating material is more than 40.0Mpa, and compressive strength is more than 51.8Mpa.
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| CN106726010B (en) * | 2016-12-14 | 2018-07-24 | 深圳先进技术研究院 | Three-dimensional bone repairing support with very low power and its preparation method and application |
| CN107441552B (en) * | 2017-08-28 | 2020-06-12 | 中国科学院上海硅酸盐研究所 | Bioactive scaffold with bionic lotus root structure and preparation method and application thereof |
| CN110301997A (en) * | 2018-03-23 | 2019-10-08 | 中国科学院化学研究所 | Biomaterial, intravascular stent and wound dressing |
| CN110151361B (en) * | 2019-01-09 | 2023-10-27 | 上海交通大学医学院附属第九人民医院 | Bone defect repairing device, preparation method thereof and slow-release pharmaceutical composition |
| CN114681688B (en) * | 2022-04-15 | 2022-11-08 | 东南大学 | Tissue regeneration membrane for promoting vascularization by using micro-channel and preparation method thereof |
Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0719529A1 (en) * | 1994-12-26 | 1996-07-03 | Kyocera Corporation | Living body-supporting member and preparation process thereof |
| CN1290182A (en) * | 1998-02-06 | 2001-04-04 | 骨科技术公司 | Osteoimplant and method for its manufacture |
| WO2003075973A1 (en) * | 2002-03-12 | 2003-09-18 | National Institute Of Advanced Industrial Science And Technology | Spherical calcium phosphate molding and use thereof |
| CN1775306A (en) * | 2005-10-10 | 2006-05-24 | 胡庆柳 | Method for preparing porous collagen composite nano hydroox apatite artificial bone |
| CN101766843A (en) * | 2010-02-05 | 2010-07-07 | 清华大学 | Artificial bone with porous laminated structure and passages and preparation method thereof |
| CN102107020A (en) * | 2011-02-14 | 2011-06-29 | 温宁 | Method for preparing artificial bone with three-dimensional bone-like structure and product prepared by method |
| CN102512709A (en) * | 2011-12-16 | 2012-06-27 | 四川大学 | Calcium phosphate-collagen nanometer fiber compound nanometer artificial bone and preparation method thereof |
| CN102641523A (en) * | 2012-03-07 | 2012-08-22 | 中南大学 | Porous hydroxyapatite biological ceramic and preparation method thereof |
| CN102935019A (en) * | 2012-10-19 | 2013-02-20 | 华中科技大学 | Gradient laminated porous scaffold based on microsphere selective laser sintering and preparation method thereof |
| CN103845762A (en) * | 2012-11-29 | 2014-06-11 | 中南大学 | Method for preparing porous bone scaffold by laser and increasing performance by adding zinc oxide |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7857860B2 (en) * | 2003-04-30 | 2010-12-28 | Therics, Llc | Bone void filler and method of manufacture |
-
2015
- 2015-12-14 CN CN201510937064.9A patent/CN105380732B/en not_active Expired - Fee Related
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0719529A1 (en) * | 1994-12-26 | 1996-07-03 | Kyocera Corporation | Living body-supporting member and preparation process thereof |
| CN1290182A (en) * | 1998-02-06 | 2001-04-04 | 骨科技术公司 | Osteoimplant and method for its manufacture |
| WO2003075973A1 (en) * | 2002-03-12 | 2003-09-18 | National Institute Of Advanced Industrial Science And Technology | Spherical calcium phosphate molding and use thereof |
| CN1775306A (en) * | 2005-10-10 | 2006-05-24 | 胡庆柳 | Method for preparing porous collagen composite nano hydroox apatite artificial bone |
| CN101766843A (en) * | 2010-02-05 | 2010-07-07 | 清华大学 | Artificial bone with porous laminated structure and passages and preparation method thereof |
| CN102107020A (en) * | 2011-02-14 | 2011-06-29 | 温宁 | Method for preparing artificial bone with three-dimensional bone-like structure and product prepared by method |
| CN102512709A (en) * | 2011-12-16 | 2012-06-27 | 四川大学 | Calcium phosphate-collagen nanometer fiber compound nanometer artificial bone and preparation method thereof |
| CN102641523A (en) * | 2012-03-07 | 2012-08-22 | 中南大学 | Porous hydroxyapatite biological ceramic and preparation method thereof |
| CN102935019A (en) * | 2012-10-19 | 2013-02-20 | 华中科技大学 | Gradient laminated porous scaffold based on microsphere selective laser sintering and preparation method thereof |
| CN103845762A (en) * | 2012-11-29 | 2014-06-11 | 中南大学 | Method for preparing porous bone scaffold by laser and increasing performance by adding zinc oxide |
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