CN105385590A

CN105385590A - Box type DNA sequenator achieving automatic test solution supply

Info

Publication number: CN105385590A
Application number: CN201510731398.0A
Authority: CN
Inventors: 任鲁风; 冯玉臣; 张睿; 蔡亦梅; 陈哲; 高静; 范东雨; 王绪敏; 王者馥; 殷金龙
Original assignee: Beijing Zhongkezixin Technology Co Ltd
Current assignee: Beijing Zhongkezixin Technology Co Ltd
Priority date: 2015-11-02
Filing date: 2015-11-02
Publication date: 2016-03-09
Anticipated expiration: 2035-11-02
Also published as: CN105385590B

Abstract

The invention relates to a box type DNA sequenator achieving automatic test solution supply. The box type DNA sequenator comprises a reaction recording subsystem, a reagent supply system, a controls system and a sequenator box for bearing the reaction recording subsystem, the reagent supply system and the controls system. The reagent supply system is arranged in a right box body, and comprises a buffer solution box loading and unloading platform assembly, a solution box lifting platform assembly and a pump valve integrated tube assembly. The buffer solution box loading and unloading platform assembly can load and unload various reagents, and the reagents are conveyed to the designated position through the vertical movement of the solution box lifting platform assembly. The pump valve integrated tube assembly selects needed reagent tubes, and the designated reagents are conveyed into a reaction bin assembly for reacting through driving of a hydraulic pump and opening and closing control over a hydraulic valve. According to the box type DNA sequenator achieving automatic rest solution supply, the solution box lifting platform assembly conveys the reagents to the designated position through guide rail movement, and control is convenient and precise. The pump valve integrated tube assembly and the buffer solution box loading and unloading platform assembly are vertically conveyed through a solution box lifting platform.

Description

Box-type DNA sequencer capable of automatically supplying test solution

Technical Field

The invention relates to the field of DNA sequencing, in particular to a box-type DNA sequencer capable of automatically supplying test solution.

Background

In the process of DNA sequencing, a chemical reaction occurs on a sequencing chip to generate visible light, and a CCD (Charge coupled device) camera captures an optical signal generated by the sequencing reaction, so that the required sequencing information can be obtained.

The reagent supply system provides various reagents for the reaction chip, and the DNA sequencer is required to be capable of storing and conveying the reagents according to requirements and is convenient to replace the reagents; in the processes of storing, conveying and replacing the reagent, the reagent supply device also has to be conveniently controlled so that the supplied reagent and the sequencing chip in the reaction bin continuously react to complete the sequencing process.

In view of the above-mentioned drawbacks, the present inventors have finally obtained the present creation through a long period of research and practice.

Disclosure of Invention

The invention aims to provide a box-type DNA sequencer with automatically supplied test solution, which is used for overcoming the technical defects.

In order to achieve the purpose, the invention provides a box-type DNA sequencer capable of automatically supplying test solution, which comprises a reaction recording subsystem, a reagent supply system, a control system and a sequencer box body for bearing the systems, wherein the reaction recording subsystem and the reagent supply system are respectively arranged in the left part and the right part of the sequencer box body and are connected through a reagent supply pipeline arranged in the box body; the control system is arranged on the rear side of the reaction recording subsystem in the left side interval of the box body;

a reagent supply system is arranged in the right box body and comprises a buffer liquid box loading and unloading platform assembly, a liquid box lifting platform assembly and a pump valve integrated pipe assembly; wherein,

the buffer liquid tank loading and unloading platform can load and unload various reagents, and the reagents are conveyed to a designated position up and down through the liquid tank lifting platform assembly and are driven by the pump valve integrated pipe assembly, the pump valve integrated pipe assembly selects a required reagent pipe, and the designated reagent is transmitted to the reaction bin assembly for reaction through the drive of the hydraulic pump and the open-close control of the hydraulic valve;

the actions of the elements are uniformly controlled by a control unit;

the box includes main part supporting component, sets up the interior welt subassembly in main part supporting component inboard, and bottom, lateral part, rear portion and the left and right sides two regional divisions of box of main part supporting component support box.

Furthermore, the liquid tank lifting platform assembly is an up-and-down reciprocating mechanism driven by a motor lead screw and is arranged in a right box body of the sequencer;

which comprises a driving motor, a supporting seat connected with the driving motor and supporting the driving motor, a screw rod component connected with the driving motor, and a reaction liquid box lifting seat arranged on the side part, wherein,

the reaction liquid tank lifting seat is internally provided with a buffer liquid tank loading and unloading platform assembly, is arranged on a mounting plate connected with the reaction liquid tank lifting seat, and bears the up-and-down motion of a reaction liquid tank lifting seat reagent pipe set under the driving of a screw rod mechanism.

Further, liquid case lift seat for reaction includes the intermediate lamella of being connected with the mounting panel, sets up the layer board in the intermediate lamella both sides, is provided with the horizontal end on the layer board for place buffer tank loading and unloading platform subassembly.

Furthermore, the pump valve pipe integrated unit comprises a pump valve integrated base plate, wherein the pump valve integrated base plate comprises a peristaltic pump mounting plate, a fixing plate connected with the peristaltic pump mounting plate, a base plate, a suction pipe hole group arranged on the base plate and a control valve assembly mounting plate in the middle of the base plate;

a peristaltic pump mounting hole is formed in the peristaltic pump mounting plate, and two peristaltic pumps are arranged on the peristaltic pump mounting hole;

and a plurality of reagent pipe clamps and wire clamps are arranged on the bottom plate at intervals.

Furthermore, the buffer tank loading and unloading platform assembly is of a drawing structure, after reagents are arranged on the platform, the whole platform assembly is installed in the right box body, when the reagents need to be loaded and unloaded, the platform is pulled out, and after the reagents are placed, the platform assembly is pushed back to the original position;

the liquid carrying platform is a pulling plate with a handle, and the handle is fixed on the pulling plate;

connecting plates are arranged on two sides of the liquid carrying platform, and the horizontal parts of the connecting plates are connected with a supporting plate of a reaction liquid box lifting seat;

under the action of the reaction liquid box lifting seat, the liquid carrying platform moves upwards to the position of the needle suction pipe on the lower side of the pump valve integrated pipe, and the reagent is selectively sucked under the control of the control unit.

Further, a sliding rail component is arranged between the liquid carrying platform and the connecting plates on the two sides, and the liquid carrying platform moves along the connecting plates.

Furthermore, a plurality of holes are arranged in parallel in the straw hole group on the bottom plate, the needle straw penetrates through the straw hole, and the other end of the needle straw is connected with a reagent tube and is connected with a liquid path port of a corresponding control valve;

the control valve assembly is installed in the middle of the integrated base plate of the pump valve, the hydraulic pump is installed on one side of the integrated base plate, and the other side is provided with a liquid path control unit which is isolated from the liquid path part through a bending plate.

Compared with the prior art, the invention has the beneficial effects that: the box-type DNA sequencer with automatically supplied test solution controls the storage, the transportation and the selection of reagents through the control unit; the liquid tank lifting platform assembly transmits the reagent to a designated position through the motion of the guide rail, and the control is convenient and accurate; the liquid tank lifting platform conveys the pump valve integrated pipe assembly and the buffer liquid tank loading and unloading platform assembly up and down to finish reagent conveying and reaction processes.

The DNA sequencer is provided with a peripheral box body structure, and sequencing reaction and reagent supply equipment has a stable and closed operating environment; the whole structure is stable, and the sequencing efficiency is high. The sequencer should have continuous and controllable characteristics during reagent supply and sequencing reaction.

The DNA sequencer has a stable structure with multiple adjustments, and is convenient to install, compact in structure and high in stability. The reaction bin assembly can be installed, fixed and adjusted, so that the reaction chip can be ensured to react in a stable environment, and can be conveniently taken out and locked.

According to the invention, the box bodies are arranged on the peripheries of the reaction bin assembly and the reagent assembly for DNA sequencing, so that the reaction bin has enough precision, stability and clean space; the space is reasonable, easily transport the adjustment.

The box body is provided with a left space and a right space which respectively contain the reaction assembly and the reagent supply and storage assembly, and the devices in the left space and the right space respectively work to form streamlined operation, so that the testing efficiency is high.

The liquid supply system of the DNA sequencer controls the storage, the transportation and the selection of the reagent through the control unit; the liquid tank lifting platform assembly transmits the reagent to a designated position through the motion of the guide rail, and the control is convenient and accurate; the liquid tank lifting platform conveys the pump valve integrated pipe assembly and the buffer liquid tank loading and unloading platform assembly up and down to finish reagent conveying and reaction processes.

Drawings

FIG. 1 is a functional block diagram of a DNA sequencer according to the present invention;

FIG. 2a is a schematic diagram of the overall structure of the case of the DNA sequencer according to the present invention;

FIG. 2b is a schematic view of a first configuration of a body support assembly of the DNA sequencer housing of the present invention;

FIG. 2c is a schematic diagram of a second configuration of a body support assembly of the DNA sequencer housing of the present invention;

FIG. 3 is a schematic diagram showing the overall structure of a reaction recording subsystem of the DNA sequencer according to the present invention;

FIG. 4 is a schematic view of a first three-dimensional structure of the reaction chamber assembly of the present invention;

FIG. 5a is a schematic view of a first three-dimensional configuration of the reversible cartridge body of the present invention;

FIG. 5b is a schematic view of a second perspective structure of the reversible cartridge body of the present invention;

FIG. 6a is a schematic perspective view of a positioning block according to the present invention;

FIG. 6b is a schematic structural diagram of the electronic lock of the present invention;

FIG. 6c is a schematic structural view of an electronic lock seat according to the present invention;

FIG. 6d is a schematic cross-sectional view of the electronic lock of the present invention;

FIG. 7 is a schematic cross-sectional view of the reversible reaction cartridge assembly of the present invention along the first lug;

FIG. 8 is a schematic view of a third perspective structure of the reversible cartridge body of the present invention;

FIG. 9a is a schematic structural view of a reaction chip assembly according to the present invention;

FIG. 9b is a schematic structural view of a reaction chip mounting base according to the present invention;

FIG. 9c is a schematic structural view of the inner side of the reaction chamber base of the present invention;

FIG. 9d is a schematic structural view of the outer side of the reaction chamber base of the present invention;

FIG. 9e is a schematic structural diagram of a cover plate of the temperature control assembly of the present invention;

FIG. 10a is a schematic cross-sectional view of a reaction chip assembly of the present invention along the liquid inlet and outlet ports;

FIG. 10b is a schematic cross-sectional view of the reversible reaction chamber assembly of the present invention along the adaptive adjustment member;

FIG. 11 is a schematic cross-sectional view of the reversible reaction chamber assembly of the present invention;

FIG. 12a is a schematic cross-sectional view of the rear cover plate of the present invention;

FIG. 12b is a perspective view of the rear cover plate assembly of the present invention;

FIG. 12c is a schematic perspective view of a rear cover plate according to the present invention;

FIG. 13a is a schematic perspective view of the top plate assembly of the DNA sequencer housing of the present invention;

FIG. 13b is a schematic perspective view of the left compartment interior trim panel assembly of the box of the DNA sequencer;

FIG. 14a is a schematic perspective view of a liquid tank lifting platform assembly according to the present invention;

FIG. 14b is a side view of the liquid tank lift platform assembly of the present invention;

FIG. 15a is a schematic perspective view of a pump valve manifold assembly according to the present invention;

FIG. 15b is a schematic top view of the pump valve manifold assembly of the present invention;

FIG. 15c is a schematic structural view of the integrated base plate of the pump valve of the present invention;

FIG. 16a is a schematic perspective view of the buffer tank loading platform assembly of the present invention;

FIG. 16b is a schematic front view of the buffer tank loading dock assembly of the present invention.

Detailed Description

The above and further features and advantages of the present invention are described in more detail below with reference to the accompanying drawings.

Please refer to fig. 1, which is a functional block diagram of a box of a DNA sequencer of the present invention, wherein the DNA sequencer of the present invention includes a reaction recording subsystem, a reagent supply system, a control system, and a box of the DNA sequencer for carrying the above systems, in the present embodiment, the reaction recording subsystem and the reagent supply system are respectively disposed in the left and right side sections of the box of the DNA sequencer and connected through a reagent supply pipeline disposed in the box; the control system is arranged at the rear side of the reaction recording subsystem in the left side interval of the box body and controls the reaction subsystem and the reagent supply system to act according to a preset time sequence.

The control system controls the reagent supply system to transmit the reagent to the reaction recording subsystem, a reaction bin assembly is arranged in the reaction recording subsystem, the reagent flows through a reaction chip arranged in the reaction bin assembly to react, and a CCD camera in the reaction recording subsystem acquires photos in the reaction process and obtains a measured DNA sequence after analysis.

Referring to fig. 2a, which is a schematic view of the overall structure of the box body of the DNA sequencer of the present invention, the box body of the DNA sequencer of the present invention includes a main body supporting component 21, a lining component disposed inside the main body supporting component 21, a left side door assembly component 23, a right side door assembly component 26, a buffer tank loading and unloading platform component 25, a left side cover plate component 30, a rear side cover plate component, and an upper cover plate 3110.

The interior lining component comprises a left side bin interior lining component 24, a right side bin left side interior lining component and a right side bin right side interior lining component 22.

In the embodiment of the invention, the box body is divided into a left part and a right part, and a reaction recording subsystem, namely a reaction bin assembly and a CCD camera, is arranged in the box body on the left side; and a reagent supply system is arranged in the right box body and comprises a pump valve pipe integrated unit, a liquid box lifting platform and a display, and a control assembly is arranged on the rear side of the box body. When the device works, the pump valve integration unit conveys reagent liquid to the reaction bin assembly in sequence to finish chemical reaction, the CCD camera finishes photographing under the control of the control unit, and finally, the result of photographing is analyzed through data to draw a conclusion.

Wherein, the main body supporting component 21 supports the bottom, the side part, the rear part and the dividing parts of the left and right box body areas of the box body; the inner side supporting components of the left box body area and the right box body area are provided with decorative plates, so that the effects of heat insulation and structural stability increase are achieved; a left side door assembly component 23 is arranged at the front part of the left side box body, and when the left side door assembly component is opened, a reaction bin component arranged in the left box body can be operated; a right door assembly 26 is provided on the right side of the right casing to operate the pump valve integrated unit when opened, and various reagent tubes and control valve lines are provided in the partial area.

Please refer to fig. 2b-2c, which are schematic structural views of the main body supporting assembly of the present invention; the main body supporting assembly is formed by splicing steel plates and is fixed in a splicing or screw fastening mode; it includes base assembly 212, top plate assembly 2112, support frame assembly 211 connected to base assembly 212 to form the side frame of the cabinet, and bumper system mount 213. The supporting frame assembly 211 includes a left vertical plate assembly 2116, a middle vertical plate assembly 2115 for separating the left box from the right box, a right vertical plate assembly 2117, a right reinforcing plate 2119 disposed at the front of the right vertical plate assembly, a rear vertical plate assembly 2118, a first cable fixing seat 2113 and a first cable fixing seat 2114 disposed on the top plate assembly 2112, and a connecting plate 2133 for connecting the assemblies together.

Wherein, the left vertical plate component 2116 and the middle vertical plate component 2115 enclose a reaction chamber accommodating cavity 200 for accommodating the reaction chamber component; a first cable fixing seat 2114 is arranged on the top plate assembly, and the left box body is divided into a reaction bin accommodating cavity 200 at the front side and a circuit board accommodating cavity 20 at the rear side by the steel plate; middle riser assembly 2115 and right riser assembly 2117 enclose reagent chamber 2001. The middle steel plate of the top plate assembly is provided with a first bending plate 2141 and a second bending plate 2142 which are integrally formed, the two sides of the steel plate are respectively bent upwards to form a part, and a wire groove is formed between the first bending plate 2141 and the second bending plate 2142 for a wire to pass through.

The buffer system mounting seat 213 comprises a platform supporting plate 2132 and a reagent tube supporting seat 2131 which are respectively arranged on the left vertical plate assembly 2116 and the middle vertical plate assembly 2115, and the platform supporting plate 2132 is provided with a platform supporting assembly and a reaction bin assembly thereon.

The box bodies are connected by plates, so that the mounting is convenient, the structural strength of the connection of all the components is high, the weight of the reaction bin component, the camera and the pump valve integrated unit can be supported, and a stable and reliable environment can be provided.

Please refer to fig. 3, which is a schematic diagram of an overall structure of a reaction recording subsystem of a DNA sequencer according to an embodiment of the present invention, the reaction recording subsystem includes a reaction chamber assembly 12, a motion platform assembly 14, a motion control unit 15, a CCD camera 101 and a platform support assembly 13, wherein,

the reaction bin assembly 12 is internally provided with a reaction chip assembly, and under the action of the control system, reaction liquid enters the reaction chip to carry out chemical reaction to generate visible light; the motion platform assembly 14 feeds the CCD camera to a proper photographing position to photograph and collect visible light information generated in the reaction chip; the platform supporting component 13 supports the reaction bin component 12 and the moving platform component 14; and the motion control unit controls the operation of the CCD camera and the reaction chip in real time.

In the embodiment of the present invention, the reaction chamber assembly 12 can mount, fix and adjust the reaction chip, so as to ensure that the reaction chip reacts in a stable environment, and the reaction chip can be conveniently taken out and locked.

Referring to fig. 4, the reaction chamber assembly 12 includes a vertically disposed door frame 121 disposed on the platform supporting assembly 13, an invertible reaction chamber assembly 124 disposed on an upper portion of an outer side of the door frame 121, an invertible base 122 connected to a lower end of the invertible reaction chamber assembly 124, the invertible base 122 fixed to a middle portion of the door frame 121, and the invertible reaction chamber assembly 124 rotatable around the invertible base 122 for taking out or placing a reaction chip.

An electronic lock assembly 125 is further disposed on the left side of the upper end of the door frame 121, and only when the electronic lock assembly 125 is opened, the reversible reaction chamber assembly 124 can be rotated to operate the reaction chip. In the embodiment of the invention, the portal 121 is arranged to support the reaction chip and related structures, so that the stability of the system is greatly improved.

The reversible reaction chamber assembly 124 comprises a reversible chamber body, and a back cover plate 1242 is arranged in the middle part of the reversible chamber body and used for packaging the reagent tube; a first rotating part is arranged at the lower part and comprises a first accommodating cavity 1243 for accommodating the reversible base 122, and the first accommodating cavity 1243 is arranged at the middle position of the lower part; first lugs 1244 symmetrically arranged are arranged at two sides of the first accommodating cavity, and a through hole 1246 is arranged in the middle of the first lugs 1244; for attachment to the reversible base 122. The reversible base can rotate in the first accommodating cavity 1243 relative to the reversible bin body, so that the stability is high, and the smooth transmission of reagents and data is ensured. The door frame 121 has a structure having a side barrier 1211 on one side, and is integrally formed.

Referring to fig. 4, the reversible base 122 of the present invention is a connection base having two second lugs 1221, the second lugs 1221 are symmetrically disposed on one side 1223 of the base, a through hole 1224 is disposed therebetween, the through hole 1246 is coaxially disposed with a through hole 1246 disposed in the middle of a first lug 1244 of the reversible cartridge body, and the second lugs 1221 are disposed inside the first lug 1244.

Referring to fig. 2, in the embodiment of the present invention, two sides of the reversible base 122 are disposed in the side barrier 1211 and are in contact with the side barrier 1211 for positioning; the first lug 1224, when coaxially mounted with the second lug 1221, contacts the upper end of the invertible base 122 and the side of the invertible cartridge contacts the inner surface of the side stop 1211 of the door 121, ensuring that the invertible cartridge and the invertible base 122 are just inside the side stop 1211.

Referring to fig. 5a and 5b, a reaction chip assembly is mounted on the inner side of the middle of the reversible bin body, correspondingly, a first cavity 1785 for accommodating a reagent tube is disposed in the middle of the reversible bin body, a first boss 1784 is disposed at the bottom of the first cavity 1785, the first boss 1784 is used for supporting a support of the reaction chip assembly, four guide bar mounting holes 1786 are disposed on the first boss 1784, and the guide bar mounting holes 1786 penetrate through two ends of the reversible bin body, the reaction chip assembly and the support to connect the reaction chip assembly with the reversible bin body; a second boss 1783 is arranged at the upper end of the first boss 1784, and the second boss 1784 is used for positioning the support; a positioning surface 1782 is arranged at the upper end of the second boss 1784, and a locking screw hole 1787 is arranged on the positioning surface 1782. The reaction chip assembly is positioned by the first cavity structure, and the reaction chip assembly cannot rotate and can only move along the guide rod when working normally.

The upper end surface of the first accommodating cavity 1243 of the reversible cabin body is provided with a reagent tube mounting hole 1788 and an electric wire mounting hole, a tube clamp 03 is mounted in the reagent tube mounting hole 1788, and a reagent tube 02 and an electric wire 01 penetrate through the tube clamp 03.

A bolt mounting hole of a bolt 1252 in the electronic lock assembly 125 is also arranged at the side part of the reversible cabin body; under the action of the locking piece, the reversible cabin body is fixed with the door frame 121.

The upper end of the outer side of the reversible cabin body is provided with a pressing piece 1241, and the reversible cabin body can be rotated only when the pressing piece 1241 is pressed. In the embodiment of the present invention, please refer to fig. 8, wherein an opening 1730 is disposed at one end of the pressing member 1241, and a claw is disposed at one end of the pressing member 1241, the claw is connected to the upper end baffle of the door frame, when the pressing member 1241 is subjected to a downward acting force, the claw is separated from the door frame baffle, and the reversible reaction cabin body can rotate relative to the door frame.

In the embodiment of the invention, the pressing part 1241 is installed in a positioning block 1762, the lower end of the positioning block 1762 is provided with a spring 1761, the spring is arranged in a spring seat 1763, and the spring seat 1763 is installed in a spring seat hole 1781 on the upper end surface of a middle through hole 1785 of a reversible reaction cabin body in a threaded manner.

Referring to fig. 5b and 6a, the positioning block 1762 is mounted in a positioning block mounting cavity 1791 at the upper end of the reversible reaction cabin, and a positioning step 1790 is formed in the cavity. The positioning block 1762 of the present embodiment includes an upper through hole 1794 and a lower through hole 1797, one of which passes through the pressing member 1241; a positioning hole 1796 and a mounting hole 1795 are formed in the side surface of the positioning block. A spring hole is provided at a lower end of the through hole where the pressing member 1241 is installed, and the spring is in contact with the bottom of the pressing member 1241 through the hole.

The embodiment of the invention is provided with the electronic lock assembly and the pressing piece, the reversible reaction bin assembly and the portal frame are locked twice, and the reaction chip is ensured to run in a safe environment and misoperation is avoided by adopting electric control and mechanical structure modes respectively.

Referring to fig. 6b and 6c, the electronic lock assembly 125 of the present invention includes an electronic lock seat and a sensing switch 1251, wherein the sensing switch is installed on the electronic lock seat, and the electronic lock seat is disposed on the side portion of the reversible cabin body, in the embodiment of the present invention, the electronic lock seat includes a side portion mounting plate 1843, and a mounting hole 1844 is disposed on the side portion mounting plate 1843; the electronic lock is fixed on the reversible bin body through the mounting hole 1844 and the mounting screw. A first housing 1841 and a second housing 1842 are provided symmetrically on the side of the side mounting plate 1843, and a space for mounting the inductive switch 1251 is left between the first housing and the second housing.

In the embodiment of the present invention, the inductive switch 1251 includes an external frame 1255, a connection threaded hole 1256 is provided on one side surface of the frame 1255, and the connection threaded hole 1256 and the electronic lock seat are fixed by a connection screw 1254; the connection screw 1254 is connected to the first frame body 1841 and the second frame body 1842 through a lug 1258 on the side surface, so as to increase the connection strength of the electronic lock seat. The inductive switch 1251 is further connected to a control circuit 1252 for controlling the inductive switch 1251.

Referring to fig. 6d, the electronic lock assembly 125 is installed at the side of the reversible bin body and the door frame 121, and the bolt 1253 extends out and enters the bolt hole 1247 formed at the side of the reversible bin body to lock; the reversible cartridge body can only be rotated when the deadbolt 1253 is electrically stimulated to retract from the deadbolt opening 1247.

The use of the portal not only can stabilize the structure of the reaction chip assembly, but also can make the reversible bin body and the reversible base compact in structure and convenient to operate; the CCD camera and the reaction chip are more accurately positioned.

A first mounting hole 1210 is formed at the lower part of the door frame 121 and is used for connecting and fixing the moving platform assembly 14; the middle part of the door frame is also provided with a second mounting hole connected with the side part of the turnable base.

Referring to fig. 7, in the embodiment of the present invention, in order to save the space and ensure the stability of reagent delivery, a first reagent tube fixing member is provided in the through hole 1246 provided in the middle of the first lug 1244 and the through hole of the second lug 1221, which includes a line coupler 1265 disposed in a left hand boss, a first washer 1264, a first rotating shaft 1261, and a reagent tube fitting 1266, a second washer 1267, a second rotation shaft 1269 disposed in the right hand lug, wherein the first washer 1264 and the second washer 1267 are arranged between the invertible cabin body and the invertible base, in this embodiment, the diameter of the through hole 1246 provided in the middle of the first lug 1244 is smaller than that of the through hole of the second lug 1221, the inner ends of the two are formed with positioning ribs for positioning the washers, and the inner diameters of the washers are just the same as the diameter of the through hole of the second lug 1221 after the two washers are mounted. The positioning flange and the gasket are arranged in the embodiment of the invention, so that no gap is left between the reversible bin body and the reversible base, the stability of the reversible bin body and the reversible base is good after the two are combined, the two can not shake due to the gap, in addition, the contact area of the two is increased by the gasket, the stress between the two is reduced, and the service life of the equipment is prolonged.

A first rotating shaft 1261 and a second rotating shaft 1269 are mounted on the inner side of a through hole 1246 arranged in the middle of the first lug 1244 and the inner side of the washer, and the two rotating shafts have the same structure and are hollow shafts with positioning convex rings; a positioning convex ring is arranged at one end of the rotating shaft, a connecting threaded hole is arranged on the positioning convex ring and is used for being connected and fixed with a lug connecting part 1263 of the reversible cabin body, and correspondingly, a positioning notch for positioning the positioning convex ring is arranged at the end part of the through hole 1246. A recess is formed in the middle of each end of the rotary shaft 1269, and the inner recess is used for positioning the line connector 1265 and the reagent connector 1266. Electric wires and reagent tubes 02, which lead to the reaction chip module, are respectively installed in the line connector 1265 and the reagent tube connector 1266. Because the components which can be mutually overturned are arranged in the embodiment of the invention, in order to avoid influencing the pipe fittings during overturning, the reagent pipe is arranged in the through hole, and the safety performance of the reagent pipe is greatly improved.

Referring to fig. 8, in the embodiment of the present invention, the reaction chip assembly is installed inside the reversible bin body. An observation part 1724 contacting with the CCD camera is arranged at the inner side of the reaction chip assembly, and a transparent baffle is arranged on the observation part so as to photograph the reaction process.

Referring to FIG. 9a, the reaction chip assembly of the present invention includes a reaction chip mounting seat 127, a reaction chamber base assembly 126, and a light-shielding positioning assembly 128 for locking and unlocking the reaction chamber base assembly 126 and the reaction chip mounting seat 127; in order to facilitate the removal and closing of the reaction chip, the reaction chip mounting block 127 can be rotated relative to the reaction chamber base assembly 126.

Referring to fig. 9b, which is a schematic structural view of the reaction chip mounting base of the present invention, a reaction chip is mounted in the middle of the reaction chip mounting base 127, and a cover plate 1277 is disposed on the reaction chip; slide groove pressing plates 1279 are installed at both sides of the reaction chip to fix the reaction chip. A mounting seat supporting component is arranged at one side edge part of the reaction chip mounting seat 127 and used for supporting the reaction chip mounting seat when a chip is taken out or placed; the reaction chip mounting base comprises a torsion spring supporting part 1273 which is arranged on the mounting base and used for supporting the torsion spring, wherein the torsion spring supporting part 1273 is a notch with a protruding end 1274, when the reaction chip mounting base 127 is rotated, the protruding end 1274 is firstly contacted with the 1261, and in the embodiment, the protruding end 1274 is arranged in the middle of the edge of the notch and the chip mounting part; the reaction chip mounting seat is supported by the structure of the mounting seat, so that the reliability is better, and the chips can be safely taken out and placed.

The mounting seat supporting assembly further comprises a torsion spring 1272 and tensioning shafts 1271 which are connected with the torsion spring 1272 and arranged at two ends of the torsion spring, wherein the tensioning shafts 1271 are arranged on the bosses 1275, and when the reaction chip mounting seat is lifted, the torsion spring 1272 supports the mounting seat and finally supports the reaction chip mounting seat by means of the positioning of the bosses 1274.

A first rotation shaft hole 1276 is further formed at an end of the protrusion 1275 to be connected to the reaction chamber base 1261. A positioning groove 1278 is further provided at the side of the reaction chip holder.

Please refer to fig. 9c, which is a schematic structural diagram of an inner side surface of a reaction chamber base according to the present invention, wherein a reaction tank 1266 is disposed in the inner side surface of the reaction chamber, a reaction tank 1267 is disposed in a middle portion of the reaction tank 1266, and a sealing ring is disposed around 1706 of the reaction tank 1267 to prevent a reaction reagent from leaking out. A protrusion 1705 is disposed at one end of the reaction chamber base 1261 for positioning with the installed cover plate. The other end is provided with a torsion spring supporting structure corresponding to the reaction chip mounting seat, the torsion spring supporting structure comprises two symmetrically arranged protruding ends 1268, a second rotating shaft hole 1269 is arranged on the torsion spring supporting structure and is coaxial with the first rotating shaft hole 1276, and the rotating shaft sequentially passes through the two holes; also provided at this end are a region 1260 in which the torsion spring is movable and a locating region 1707 in which a threaded hole 1712 is provided to which the reaction chamber cover plate will be mounted.

Please refer to fig. 9d, which is a schematic structural diagram of an outer side surface of a reaction chamber base according to the present invention, wherein a temperature control assembly is disposed at a middle portion of the outer side surface of the reaction chamber base, a temperature sensor mounting groove 1262 is disposed at the middle portion of the reaction chamber base, and a heating wire mounting groove 1704 is disposed at a bottom of the mounting groove 1262, in which a heating wire and a temperature sensor are respectively disposed. A positioning boss 1703 is also provided in the mounting slot for positioning with the temperature control assembly cover plate 1263 when mounted. A positioning notch 1702 corresponding to the positioning boss 1703 is provided on the temperature control assembly cover plate 1263.

Four guide rod mounting holes 1742 are arranged around the temperature sensor mounting groove 1262, and self-adaptive spring mounting holes 1741 are arranged at the upper and lower sides; the cover plate 1263 is also provided with a guide bar hole 1701.

A light-shielding positioning assembly mounting groove 1265 is further formed on the reaction chamber base for mounting the light-shielding positioning assembly 128, and a mounting boss 1744 is arranged therein.

Referring to fig. 10a-10b, an electric heating wire 1754 is disposed in the middle of the base of the reaction chamber and disposed in the heating wire mounting groove 1704 of the base; a temperature sensor 1720 and a control component are arranged in the temperature sensor mounting groove 1262 at the upper part of the electric heating wire, and are used for detecting the temperature of the reaction tank and adjusting the temperature according to a set threshold value; a seal 1263, which is a potting resin, is also molded into the temperature sensor 1720 and the control module 1753. Reagent tube mounting threaded holes 1721 are respectively formed in two ends of the reaction tank and are used for being connected with reagent tubes; the end of the reagent tube mounting threaded hole 1721 is provided with a liquid inlet 1723 and a liquid outlet 1713, and the liquid inlet and the liquid outlet are communicated with two ends of the reaction tank for circularly detecting reagents.

A sealing ring 1713 is arranged on the side of the inner groove of the reaction tank. A reaction chip 04 is mounted in the middle of the reaction chip mounting base, and a reagent flows through the reaction chip to react.

In the embodiment of the invention, the reagent tube 02 passes through the reagent tube mounting hole 1788 of the reversible bin body, the first cavity 1785 for accommodating the reagent tube is arranged in the middle of the reversible bin body and is finally connected with the reaction tank body, and the reagent tube is sealed by various cavities, so that the stability is high and the damage is not easy to damage.

A rear cover plate 1242 is fixed on the rear side of the reaction chamber body, a self-adaptive adjusting piece 1751 is arranged in the middle of the rear cover plate 1242, please refer to fig. 12a-12c, two rows of mounting convex strips 1809 are arranged in the middle of the inner side of the rear cover plate 1242, a first mounting boss 1812 and a second mounting boss 1811 are arranged in the middle of the mounting convex strips 1809, a threaded hole 1802 is formed in the mounting boss, and the self-adaptive adjusting piece 1751 is installed in the threaded hole 1802.

In the embodiment of the invention, the connecting end of the adaptive adjusting piece 1751 and the rear cover plate is a threaded end, the connecting end of the adaptive adjusting piece 1751 and the reaction bin assembly is a polished rod 1805 provided with a spring 1806, a nut 1804 is arranged between the two ends for positioning, and after the installation is finished, the nut 1804 is tightly close to the installation boss.

A channel 1803 is arranged between the two convex strips 1809, so that a reagent tube can pass through the channel; a reinforcing rib plate 1812 vertical to the raised line 1809 is also arranged on the rear cover plate; the upper end surface 1810 of the convex strip 1809 and the boss is located on a plane; fastening screw holes 1801 are arranged in the middle and four corners of a peripheral frame body 1807 of the rear panel and are used for being connected and fixed with the reversible reaction cabin body.

Above-mentioned back shroud subassembly not only can carry out face seal to the reaction storehouse subassembly, can also install fixed self-adaptation regulating part, simple structure.

In the embodiment of the invention, a self-adaptive adjusting piece 1751 is arranged between the reversible reaction bin body and the reaction bin base 126, and a spring at the tail end of the self-adaptive adjusting piece 1751 is contacted with the end surface of the reaction bin base; when using the CCD camera to feed, when camera and reaction chip subassembly contact, when adjusting the camera position, can adopt the mode adjustment of back-and-forth movement, at this moment, self-adaptation regulating part can change the position of reaction chip subassembly according to the position change of camera, guarantees to have higher detection accuracy, simultaneously, avoids causing the damage to the system.

Referring to fig. 13a-13b, a top plate assembly 2112 in an embodiment of the present invention includes a front side plate 2124, a right side plate 2125, a rear side plate 2143, and a left side plate 2153, which are sequentially connected to each other, and each plate is a bent plate including a vertical plate and a horizontal plate, the vertical plate is disposed outside the horizontal plate, and the horizontal plate is connected to each other in a front-rear direction to form a supporting portion.

An intermediate plate 2123 is connected between the front side plate 2124 and the rear side plate 2143, and a spacer plate 2128 is provided between the intermediate plate 2123 and the left side plate 2153; a fluid path receiving chamber is formed between the middle plate 2123 and the right side plate 2143, and a reaction chamber receiving chamber is formed between the front side plate 2124 and the partition plate 2128.

In the present embodiment, the upper end of the top plate assembly 2112 is connected to the upper cover plate 3110, and the upright portion of the right side plate 2125 is provided with an integrally formed connecting piece 2151; a connecting plate 2133 is provided at the vertical portion of the rear side plate 2143.

The middle plate 2123 is provided with a wire groove formed by a bent portion thereof, which includes a rear side wire groove 2127, and a wire is led into the reaction chamber assembly from the rear side wire groove 2127 through the first cable fixing seat 2114. A front connecting part 2122 is arranged on the front side, a left connecting part 2121 is arranged on the left side, and a threaded hole is formed in the left connecting part 2121 and used for being connected with an upper cover plate; a first rear connecting part 2130 on the left side and a second rear connecting part 2129 on the rear side; the second rear connecting portion 2129 is provided with a latch 2150.

A connection portion 2152 is provided at a corner of the top plate.

The left side cabin interior trim panel assembly component of the present invention includes a first interior trim panel 241 disposed in the middle, and a second interior trim panel 242 and a third interior trim panel 243 disposed at both sides of the first interior trim panel 241 and connected thereto, respectively, and a first interior trim thickening plate 244 and a second interior trim thickening plate 245 are further connected to the outer side of the second interior trim panel 242.

The reagent supply system comprises a control unit, a buffer liquid tank loading and unloading platform assembly, a liquid tank lifting platform assembly and a pump valve integrated tube assembly, wherein the buffer liquid tank loading and unloading platform can load and unload various reagents, the reagents are conveyed to a specified position through the liquid tank lifting platform assembly up and down and are driven by the pump valve integrated tube assembly, the pump valve integrated tube assembly selects a required reagent tube, and the specified reagent is transmitted to a reaction bin assembly for reaction through the driving of a hydraulic pump and the opening and closing control of the hydraulic valve; the operations of the above-mentioned respective elements are collectively controlled by a control unit.

The liquid tank lifting platform assembly provided by the embodiment of the invention is an up-and-down reciprocating mechanism driven by a motor lead screw and is arranged in a right box body of a sequencer. The device comprises a driving motor 401, a supporting base 402 which is connected with the driving motor and supports the driving motor, a screw assembly which is connected with the driving motor, and a reaction liquid tank lifting base 408 which is arranged on the side part, wherein a buffer liquid tank loading and unloading platform assembly is arranged in the reaction liquid tank lifting base 408, the reaction liquid tank lifting base 408 is arranged on a mounting plate 404 which is connected with the reaction liquid tank lifting base, and a reaction liquid tank lifting base reagent tube group is driven by the screw mechanism to move up and down.

Reaction is with liquid case lift seat 408 includes the intermediate lamella 4081 of being connected with the mounting panel, sets up the layer board 4082 in the intermediate lamella both sides, is provided with the horizontal end on the layer board 4082 for place buffer tank loading and unloading platform subassembly.

An output shaft of the driving motor 401 is connected with a lead screw 403, the lead screw is arranged in a base 405, and the base 405 is connected with the end part of the driving motor 401 through a supporting seat 402; a stroke detection switch 406 is provided on a side portion of the base 405. An output shaft of the driving motor is connected with a connecting end 4034 of the lead screw through a coupler 4035; the screw rod is connected with a nut 4031 which is fixed on a nut seat 4032; the lead screw lower extreme passes through the bearing support, and the bearing end is provided with bearing end cover 4011.

According to the embodiment of the invention, the liquid tank lifting platform assembly transfers the reagent to the designated position through the motion of the guide rail, so that the control is convenient and accurate; the liquid tank lifting platform conveys the pump valve integrated pipe assembly and the buffer liquid tank loading and unloading platform assembly up and down to finish reagent conveying and reaction processes.

The pump valve integrated pipe assembly of the embodiment of the invention sucks the reagent from the reagent pipe on the buffer liquid tank loading and unloading platform assembly through the needle suction pipe 507 on the lower side, and drives the control valve assembly 509 and the hydraulic pump 506 to select through the selection of the liquid path control unit 502, so that the reagent is discharged into the reagent pipeline for a sequencing reaction.

The pump and valve integrated tube assembly comprises a pump and valve integrated base plate 508, wherein the pump and valve integrated base plate 508 comprises a peristaltic pump mounting plate 5011, a fixing plate 5083 connected with the peristaltic pump mounting plate 5011, a bottom plate 5084, a suction pipe hole group 5085 arranged on the bottom plate and a control valve assembly mounting plate 5081 in the middle of the bottom plate; a peristaltic pump mounting hole 5082 is formed in the peristaltic pump mounting plate 5011, and two peristaltic pumps 505 are arranged on the peristaltic pump mounting hole; a plurality of reagent tube clamps and wire clamps 5015 are also spaced apart on the base 5084.

Accordingly, the control valve assembly 509 is installed at a middle portion of the pump valve integrated base plate 508, the hydraulic pump 506 is installed at a side portion thereof, and the fluid path control unit 502 is provided at the other side portion, and the fluid path control unit 502 is separated from the fluid path portion by a bending plate 503.

In the embodiment of the invention, in order to perform sequencing reaction on a plurality of reagents, a plurality of holes are arranged in parallel in the straw hole group 5085 on the bottom plate, the needle straw 507 passes through the straw hole, and the other end of the needle straw is connected with a reagent pipe and is connected with a liquid path port of a corresponding control valve; a tube clamp 5013 is provided on the tube hole to prevent the needle tubing from tilting.

According to the embodiment of the invention, the pump valve integrated pipe assembly is adopted, so that multiple reagents can be simultaneously selected, sequencing reaction is carried out according to a preset sequence, and the testing efficiency is high.

Buffer tank loading platform subassembly is the structure of pull formula, sets up reagent back on the platform, and whole platform unit mount is in the right side box, when needs loading and unloading reagent, pulls out the platform, places the back, pushes back the original position again.

It includes a liquid carrying platform 310, which in this embodiment is a pull plate with a handle 3101, and the handle is fixed on the pull plate; connecting plates 311 are arranged on two sides of the liquid carrying platform 310, and the horizontal part of the connecting plate 311 is connected with the supporting plate of the reaction liquid box lifting seat 408; under the action of the reaction liquid tank lifting seat, the liquid carrying platform 310 moves upwards to the needle suction tube 507 at the lower side of the pump valve integrated tube, and the reagent is selectively sucked under the control of the control unit.

Between the liquid bearing platform 310 and the connecting plates 311 on both sides, a sliding rail assembly 312 is disposed, which enables the liquid bearing platform to move along the connecting plates.

The above detailed description is specific to one possible embodiment of the present invention, and the embodiment is not intended to limit the scope of the present invention, and all equivalent implementations or modifications without departing from the scope of the present invention should be included in the technical scope of the present invention.

Claims

1. A box-type DNA sequencer capable of automatically supplying test solution is characterized by comprising a reaction recording subsystem, a reagent supply system, a control system and a sequencer box body for bearing the systems, wherein the reaction recording subsystem and the reagent supply system are respectively arranged in a left part interval and a right part interval of the sequencer box body and are connected through a reagent supply pipeline arranged in the box body; the control system is arranged on the rear side of the reaction recording subsystem in the left side interval of the box body;

the actions of the elements are uniformly controlled by a control unit;

2. The box-type DNA sequencer with automatic test solution supply of claim 1, wherein the liquid box lifting platform assembly is a motor screw-driven up-and-down reciprocating mechanism which is arranged in a right box body of the sequencer;

3. The chamber-type DNA sequencer as set forth in claim 2, wherein the reaction solution chamber elevator comprises a middle plate connected to the mounting plate, and support plates disposed on both sides of the middle plate and having horizontal ends for receiving the buffer solution chamber platform assembly.

4. The chamber-type DNA sequencer of claim 2, wherein the pump-valve tube assembly unit comprises a pump-valve assembly base plate, the pump-valve assembly base plate comprises a peristaltic pump mounting plate, a fixing plate connected to the peristaltic pump mounting plate, a base plate, a set of pipette holes provided on the base plate, and a control valve assembly mounting plate in the middle of the base plate;

5. The box-type DNA sequencer capable of automatically supplying test solutions according to claim 2, wherein the buffer solution box loading and unloading platform assembly is of a pull-out structure, the whole platform assembly is installed in the right box body after reagents are arranged on the platform, and when the reagents need to be loaded and unloaded, the platform is pulled out and is pushed back to the original position after being placed;

6. The chamber type DNA sequencer of claim 5, wherein a slide rail assembly is provided between the carrier platform and the connection plates at both sides, and the carrier platform is moved along the connection plates.

7. The chamber-type DNA sequencer of claim 4, wherein a plurality of holes are arranged in parallel in the set of pipette holes on the bottom plate, the needle pipette passes through the pipette hole, and the other end of the needle pipette is connected to a reagent tube and connected to a liquid path port of a corresponding control valve;