CN105350183A - Manufacturing method and device for nano-fiber three-dimensional support - Google Patents
Manufacturing method and device for nano-fiber three-dimensional support Download PDFInfo
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Abstract
本发明公开了一种纳米纤维三维支架制备方法及装置，包括供液装置、供气装置、同轴喷头和与同轴喷头相对设置的接收装置，同轴喷头具有出气口和出液口，出气口可产生将出液口内的溶液吹气拉伸并在同轴喷头与接收装置间形成对射流进行辅助拉伸的气流，接收装置包括转轴、可驱动转轴转动的传动装置以及设在转轴上的若干支撑臂，支撑臂随转轴转动时可形成正对同轴喷头的碗状回转面。本发明在纺丝过程中无需高压电场，在气流的剪力作用下，即可以实现射流拉伸，获得纳米纤维，安全可靠，材料兼容性好，尤其适用于导电性差或具有生物活性的材料。同时，相比传统平板收集器或封闭式收集器，可有效地使高速气流通过，避免产生反冲气流。
The invention discloses a preparation method and device for a nanofiber three-dimensional support, comprising a liquid supply device, an air supply device, a coaxial nozzle and a receiving device opposite to the coaxial nozzle. The air port can generate air blowing and stretching the solution in the liquid outlet and form an airflow between the coaxial nozzle and the receiving device to assist the stretching of the jet. The receiving device includes a rotating shaft, a transmission device that can drive the rotating shaft to rotate, and an airflow installed on the rotating shaft. A plurality of support arms, when the support arms rotate with the rotating shaft, can form a bowl-shaped rotating surface facing the coaxial nozzle. The invention does not need a high-voltage electric field in the spinning process, and under the shear force of the airflow, jet stretching can be realized to obtain nanofibers, which is safe and reliable, and has good material compatibility, and is especially suitable for materials with poor conductivity or biological activity. At the same time, compared with traditional flat collectors or closed collectors, it can effectively allow high-speed airflow to pass through and avoid backlash airflow.
Description
技术领域 technical field
本发明用于那么纤维支架技术领域，特别是涉及一种纳米纤维三维支架制备方法及装置。 The invention is used in the technical field of so-fibrous scaffolds, and in particular relates to a preparation method and device for a nanofiber three-dimensional scaffold.
背景技术 Background technique
近年来，三维纳米结构在组织工程等领域等到人们的高度关注和深入研究。组织工程的原理是诱导和促进细胞在体外或体内的生长、迁移以及增殖等一系列生理活动，最终形成具有三维结构的器官或组织。研究者利用工程学方法制造仿生结构，模拟天然组织的生理环境，包括结构的、物理的以及形态的特征。其中微观结构，对细胞在支架内的增殖、黏附、向内生长形成组织、营养物质及代谢产物的良好运输，以及对最终的组织构建起着至关重要的作用。 In recent years, three-dimensional nanostructures have received great attention and in-depth research in the fields of tissue engineering and other fields. The principle of tissue engineering is to induce and promote a series of physiological activities such as cell growth, migration and proliferation in vitro or in vivo, and finally form organs or tissues with three-dimensional structures. Researchers use engineering methods to create biomimetic structures that mimic the physiological environment of natural tissues, including structural, physical, and morphological characteristics. Among them, the microstructure plays a vital role in the proliferation, adhesion, inward growth of cells in the scaffold to form tissues, the good transportation of nutrients and metabolites, and the final tissue construction.
目前，制备纳米纤维组织工程支架多采用静电纺丝法。静电纺丝方法具有简便快捷、成本低廉等优点，有望成为理想的组织工程仿生支架的制备方法。但是，传统静电纺丝方法用于高效制备可控三维结构仍然存在一定的技术瓶颈。 At present, electrospinning is mostly used to prepare nanofibrous tissue engineering scaffolds. The electrospinning method has the advantages of simplicity, quickness, and low cost, and is expected to become an ideal method for preparing biomimetic scaffolds for tissue engineering. However, there are still certain technical bottlenecks in the efficient preparation of controllable three-dimensional structures by the traditional electrospinning method.
静电纺丝制备纤维支架具有如下局限性： The preparation of fibrous scaffolds by electrospinning has the following limitations:
1、随着薄膜支架厚度增加，喷头与收集板之间的电场将逐渐减弱，导致薄膜支架上表面电荷不断积累，最终导致电纺过程终止，限制了所能获得支架的厚度（通常为微米级厚度），制造大厚度的真正意义的三维结构仍然较为困难。 1. As the thickness of the film support increases, the electric field between the nozzle and the collecting plate will gradually weaken, resulting in the continuous accumulation of surface charges on the film support, which eventually leads to the termination of the electrospinning process, limiting the thickness of the support that can be obtained (usually micron level Thickness), it is still difficult to manufacture a real three-dimensional structure with a large thickness.
2、传统的静电纺丝是在电场的作用下，逐层被接地收集板吸引堆积，支架的纤维排列紧密、纤维之间的空隙过小，使细胞难以长入，无法构建出令人满意的三维组织。 2. The traditional electrospinning is under the action of an electric field, which is attracted and accumulated layer by layer by the grounded collecting plate. The fibers of the scaffold are closely arranged and the gaps between the fibers are too small, which makes it difficult for cells to grow in and cannot construct a satisfactory structure. three-dimensional organization.
3、传统喷头式静电纺丝效率低下，单喷头静电纺丝产量通常只有0.1-1g/h,难以实现大规模、高效率的三维支架生产制造。 3. The efficiency of traditional nozzle-type electrospinning is low, and the output of single-nozzle electrospinning is usually only 0.1-1g/h, which makes it difficult to realize large-scale and high-efficiency three-dimensional scaffold production.
4、静电纺丝需要高压电场，对于溶液材料的导电性有一定的要求，选择的材料必须对高压电场有一定的承受能力，不适用于打印导电率差的材料或者具有生物活性的材料，具有材料局限性。 4. Electrospinning requires a high-voltage electric field, and there are certain requirements for the conductivity of the solution material. The selected material must have a certain ability to withstand the high-voltage electric field. It is not suitable for printing materials with poor conductivity or materials with biological activity. Material limitations.
高速气吹聚合物溶液进行大规模制备纳米纤维的技术（高速气流溶液纺丝技术），可以使纳米纤维的制备速度较原来的静电纺丝速度提高10倍以上，但是，传统气纺多采用封闭式接收器或滚筒式接收器，在高速气流作用下，容易产生反冲气流，影响纤维的定向沉积，如何采用高速气纺制备具有三维蓬松结构的纳米纤维支架，仍然没有通用性的工艺。使用旋转敞开式接收器进行高速气流纺丝纤维收集和三维纤维支架的制备，尚未见报道。 The technology of large-scale preparation of nanofibers by high-speed air-blowing polymer solution (high-speed airflow solution spinning technology) can increase the preparation speed of nanofibers by more than 10 times compared with the original electrospinning speed. Under the action of high-speed airflow, it is easy to generate recoil airflow and affect the directional deposition of fibers. How to prepare nanofiber scaffolds with three-dimensional fluffy structure by high-speed air spinning is still not a universal process. The use of rotating open receivers for high-speed air-spun fiber collection and preparation of three-dimensional fiber scaffolds has not been reported.
发明内容 Contents of the invention
为解决上述问题，本发明提供一种纳米纤维三维支架制备方法及装置，其工艺简单，成本较低，适于用不同的纺丝材料制备不同力学强度、生物相容性及降解性能的三维纤维支架，并且可以通过气流纺丝的方法直接制备大量微纳米纤维支架。 In order to solve the above problems, the present invention provides a method and device for preparing a nanofiber three-dimensional scaffold, which has a simple process and low cost, and is suitable for preparing three-dimensional fibers with different mechanical strengths, biocompatibility and degradation properties by using different spinning materials Scaffolds, and a large number of micro-nanofiber scaffolds can be directly prepared by air spinning.
本发明解决其技术问题所采用的技术方案是：一种纳米纤维三维支架制备装置，包括供液装置、供气装置、同轴喷头和与所述同轴喷头相对设置的接收装置，所述同轴喷头具有与供气装置连接的出气口和与供液装置连接的出液口，所述出气口可产生将出液口内的溶液吹气拉伸并在同轴喷头与接收装置间形成对射流进行辅助拉伸的气流，所述接收装置包括转轴、可驱动所述转轴转动的传动装置以及设在所述转轴上的若干支撑臂，支撑臂随转轴转动时可形成正对所述同轴喷头的碗状回转面。 The technical solution adopted by the present invention to solve the technical problem is: a nanofiber three-dimensional support preparation device, including a liquid supply device, an air supply device, a coaxial nozzle and a receiving device opposite to the coaxial nozzle. The axial spray head has an air outlet connected to the air supply device and a liquid outlet connected to the liquid supply device. The air outlet can blow and stretch the solution in the liquid outlet and form a jet flow between the coaxial spray head and the receiving device. The airflow for auxiliary stretching, the receiving device includes a rotating shaft, a transmission device that can drive the rotating shaft to rotate, and a number of support arms arranged on the rotating shaft. bowl-shaped rotary surface.
进一步作为本发明技术方案的改进，所述支撑臂采用弯曲的金属片或金属杆或塑料杆，各所述支撑臂均匀的分布在所述转轴上并形成碗状的爪结构。 As a further improvement of the technical solution of the present invention, the support arms are bent metal sheets or metal rods or plastic rods, and each of the support arms is evenly distributed on the rotating shaft and forms a bowl-shaped claw structure.
进一步作为本发明技术方案的改进，所述同轴喷头内设有气室，同轴喷头上设有伸入所述气室内的喷液头，所述喷液头在气室内的一端设有出液口，喷液头的另一端通过进液管与供液装置相连，所述气室上在出液口的对侧设有出气口，所述供气装置通过设有连接块的进气管与所述气室连接。 As a further improvement of the technical solution of the present invention, the coaxial spray head is provided with an air chamber, the coaxial spray head is provided with a liquid spray head extending into the air chamber, and one end of the liquid spray head is provided with an outlet in the air chamber. The other end of the liquid spray head is connected to the liquid supply device through the liquid inlet pipe. The air chamber is provided with an air outlet on the opposite side of the liquid outlet. The air supply device is connected to the liquid supply device through the inlet pipe provided with the connecting block. The air chamber is connected.
进一步作为本发明技术方案的改进，所述出液口和出气口在同一轴线上，出气口的孔径大于出液口的孔径。 As a further improvement of the technical solution of the present invention, the liquid outlet and the gas outlet are on the same axis, and the diameter of the gas outlet is larger than that of the liquid outlet.
进一步作为本发明技术方案的改进，所述出气口的孔径为0.6mm-1mm，所述出液口的孔径为0.1mm-0.6mm。 As a further improvement of the technical solution of the present invention, the aperture of the gas outlet is 0.6mm-1mm, and the aperture of the liquid outlet is 0.1mm-0.6mm.
进一步作为本发明技术方案的改进，所述喷液头在气室内具有锥形的尖端，通过所述尖端在气室内部形成向出气口汇集的气流通道。 As a further improvement of the technical solution of the present invention, the liquid spray head has a tapered tip in the air chamber, through which an airflow channel converging to the air outlet is formed inside the air chamber.
进一步作为本发明技术方案的改进，所述供液装置包括微量注射泵，所述进气管上设有精密气压调节阀。 As a further improvement of the technical solution of the present invention, the liquid supply device includes a micro-injection pump, and a precise air pressure regulating valve is provided on the air inlet pipe.
进一步作为本发明技术方案的改进，所述传动装置包括输出端与所述转轴相连的电机。 As a further improvement of the technical solution of the present invention, the transmission device includes a motor whose output end is connected to the rotating shaft.
一种纳米纤维三维支架制备方法，包括以下步骤： A method for preparing a nanofiber three-dimensional scaffold, comprising the following steps:
S10.纺丝溶液的制备，将生物降解材料溶于溶剂中，密闭搅拌，得到纺丝溶液；将所得纺丝溶液置于供液装置中，启动供液装置，纺丝溶液由进液管进入同轴喷头的喷液头； S10. Preparation of spinning solution, dissolving the biodegradable material in a solvent, and stirring in a closed manner to obtain a spinning solution; placing the obtained spinning solution in the liquid supply device, starting the liquid supply device, and the spinning solution enters through the liquid inlet pipe The liquid spray head of the coaxial spray head;
S20.启动接收装置，接收装置包括转轴、可驱动所述转轴转动的传动装置以及设在所述转轴上的若干支撑臂，支撑臂随转轴转动时可形成碗状回转面； S20. Start the receiving device, the receiving device includes a rotating shaft, a transmission device capable of driving the rotating shaft to rotate, and a plurality of support arms arranged on the rotating shaft, and the supporting arms can form a bowl-shaped turning surface when rotating with the rotating shaft;
S30.同轴喷头上的喷液头伸入所述气室内，所述喷液头在气室内的一端设有出液口，所述气室上在出液口的对侧设有出气口，打开供气装置，调节精密气压调节阀，气体经过精密气压调节阀进行气压调节后，流经进气管进入同轴喷头的气室，最终从出气口处喷出，并产生将喷液头的出液口内的溶液吹气拉伸并在同轴喷头与接收装置间形成对射流进行辅助拉伸的气流，在气流的持续作用下，实现射流拉伸，获得纳米纤维； S30. The liquid spray head on the coaxial spray head extends into the air chamber, the liquid spray head is provided with a liquid outlet at one end of the air chamber, and the air chamber is provided with an air outlet on the opposite side of the liquid outlet, Open the air supply device and adjust the precision air pressure regulating valve. After the air pressure is adjusted by the precision air pressure regulating valve, the gas flows through the air inlet pipe and enters the air chamber of the coaxial nozzle, and finally sprays out from the air outlet, and produces the output of the liquid ejection head. The solution in the liquid port is blown and stretched, and an airflow that assists the stretching of the jet is formed between the coaxial nozzle and the receiving device. Under the continuous action of the airflow, the jet stretching is realized to obtain nanofibers;
S40.在气流推力作用下，纳米纤维往接收装置方向沉积，同时，气流从接收装置的侧端导出，并辅助纤维在接收装置上沉积成为三维支架结构。 S40. Under the thrust of the airflow, the nanofibers are deposited toward the receiving device, and at the same time, the airflow is guided from the side of the receiving device, and assists the deposition of the fibers on the receiving device to form a three-dimensional scaffold structure.
本发明的有益效果：本发明在纺丝过程中无需高压电场，在气流的剪力作用下，即可以实现射流拉伸，获得纳米纤维，安全可靠，材料兼容性好，尤其适用于导电性差或具有生物活性的材料。同时，由于生产原理有本质创新，相比静电纺丝，无残余电荷积累和电场积累等因素造成的厚度限制，可获得极高厚度的三维支架结构。而且，在高速气流的作用下，生产效率极大地提高，是传统静电纺丝的10-40倍。 Beneficial effects of the present invention: the present invention does not need a high-voltage electric field in the spinning process, and under the action of the shear force of the airflow, jet stretching can be realized to obtain nanofibers, which is safe and reliable, and has good material compatibility, especially suitable for poor electrical conductivity or biologically active material. At the same time, due to the essential innovation of the production principle, compared with electrospinning, there is no thickness limitation caused by factors such as residual charge accumulation and electric field accumulation, and a three-dimensional scaffold structure with extremely high thickness can be obtained. Moreover, under the action of high-speed airflow, the production efficiency is greatly improved, which is 10-40 times that of traditional electrospinning.
同时，纳米纤维三维支架制备装置根据线动成面的思想，创新地采用旋转的敞开式接收装置，相比传统平板收集器或者封闭式收集器，可有效地使高速气流通过，从而避免产生反冲气流，影响纤维沉积。相反地，高速气流将帮助纤维在收集器上沉积成为三维支架结构。传统的静电纺丝是在电场的作用下，逐层被接地收集板吸引堆积，支架的纤维排列紧密、纤维之间的空隙过小，使细胞难以长入，无法构建出令人满意的三维组织。而采用气流拉升形成纤维并辅助沉积，可获得疏松、低密度的结构，更有利于细胞生长。 At the same time, the nanofiber three-dimensional scaffold preparation device innovatively adopts a rotating open receiving device based on the idea of linear movement into a surface, which can effectively allow high-speed airflow to pass through compared with traditional flat collectors or closed collectors, thereby avoiding reaction. Impulse air flow affects fiber deposition. Conversely, the high-velocity gas flow will help deposit the fibers into a three-dimensional scaffold structure on the collector. Traditional electrospinning is under the action of an electric field, which is attracted and accumulated layer by layer by a grounded collecting plate. The fibers of the scaffold are tightly arranged, and the gaps between the fibers are too small, making it difficult for cells to grow in, and it is impossible to construct a satisfactory three-dimensional tissue. . However, the use of airflow to form fibers and assist in deposition can obtain a loose, low-density structure, which is more conducive to cell growth.
通过本发明获得的纳米纤维三维支架具有优越的蓬松结构，同时还具有良好的生物相容性、机械性能和生物可降解性能，为细胞外基质建立提供良好的环境，有利于细胞的繁殖和组织的愈合，将推动组织工程领域在模拟体内环境发展。 The nanofiber three-dimensional scaffold obtained by the present invention has a superior fluffy structure, and also has good biocompatibility, mechanical properties and biodegradability, and provides a good environment for the establishment of extracellular matrix, which is conducive to cell reproduction and tissue The healing of the tissue engineering field will promote the development of simulated in vivo environment.
附图说明 Description of drawings
下面结合附图对本发明作进一步说明： The present invention will be further described below in conjunction with accompanying drawing:
图1是本发明结构示意图； Fig. 1 is a structural representation of the present invention;
图2是本发明同轴喷头结构示意图。 Fig. 2 is a schematic diagram of the structure of the coaxial nozzle of the present invention.
具体实施方式 detailed description
参照图1、图2，其显示出了本发明之较佳实施例的具体结构。以下将详细说明本发明各元件的结构特点，而如果有描述到方向(上、下、左、右、前及后)时，是以图1所示的结构为参考描述，但本发明的实际使用方向并不局限于此。 With reference to Fig. 1, Fig. 2, it has shown the specific structure of preferred embodiment of the present invention. The structural features of each element of the present invention will be described in detail below, and if there is description to direction (up, down, left, right, front and back), it is described with reference to the structure shown in Fig. 1, but the actual The direction of use is not limited to this.
本发明提供了一种纳米纤维三维支架制备装置，包括供液装置1、供气装置2、同轴喷头3和与所述同轴喷头3相对设置的接收装置4，所述供液装置1包括微量注射泵，所述同轴喷头3具有与供气装置2连接的出气口31和与供液装置1连接的出液口32，所述出气口31可产生将出液口32内的溶液吹气拉伸并在同轴喷头3与接收装置4间形成对射流进行辅助拉伸的气流，所述接收装置4包括转轴41、可驱动所述转轴41转动的传动装置42以及设在所述转轴41上的若干支撑臂43，所述传动装置42包括输出端与所述转轴41相连的电机，所述支撑臂43采用弯曲的金属片或金属杆或塑料杆，各所述支撑臂43均匀的分布在所述转轴41上并形成碗状的爪结构。支撑臂43随转轴41转动时可形成正对所述同轴喷头3的碗状回转面。 The present invention provides a nanofiber three-dimensional scaffold preparation device, comprising a liquid supply device 1, an air supply device 2, a coaxial nozzle 3 and a receiving device 4 arranged opposite to the coaxial nozzle 3, and the liquid supply device 1 includes A micro-injection pump, the coaxial nozzle 3 has an air outlet 31 connected to the gas supply device 2 and a liquid outlet 32 connected to the liquid supply device 1, and the air outlet 31 can blow the solution in the liquid outlet 32 Air stretching and forming an airflow that assists the stretching of the jet between the coaxial nozzle 3 and the receiving device 4. The receiving device 4 includes a rotating shaft 41, a transmission device 42 that can drive the rotating shaft 41 to rotate, and is arranged on the rotating shaft. Several support arms 43 on 41, the transmission device 42 includes a motor whose output end is connected to the rotating shaft 41, the support arms 43 adopt curved metal sheets or metal rods or plastic rods, and each of the support arms 43 is uniform Distributed on the rotating shaft 41 and form a bowl-shaped claw structure. When the supporting arm 43 rotates with the rotating shaft 41 , it can form a bowl-shaped rotating surface facing the coaxial spray head 3 .
所述同轴喷头3内设有气室33，同轴喷头3上设有伸入所述气室33内的喷液头34，所述喷液头34在气室33内的一端设有出液口32，喷液头34的另一端通过进液管与供液装置1相连，所述气室33上在出液口32的对侧设有出气口31，所述供气装置2通过设有连接块36的进气管与所述气室33连接，所述进气管上设有精密气压调节阀37。所述出液口32和出气口31在同一轴线上，出气口31的孔径大于出液口32的孔径，作为优选，所述出气口31的孔径为0.6mm-1mm，所述出液口32的孔径为0.1mm-0.6mm。所述喷液头在气室33内具有锥形的尖端，通过所述尖端在气室33内部形成向出气口31汇集的气流通道35，由气流通道35吹出的气流包裹住出液口32，从而在高速气流的剪力作用下，实现射流拉伸，获得纳米纤维。 The coaxial spray head 3 is provided with an air chamber 33, the coaxial spray head 3 is provided with a liquid spray head 34 extending into the air chamber 33, and one end of the liquid spray head 34 in the air chamber 33 is provided with an outlet The liquid port 32, the other end of the liquid spray head 34 is connected with the liquid supply device 1 through the liquid inlet pipe, and the opposite side of the liquid outlet 32 is provided with an air outlet 31 on the described air chamber 33, and the described air supply device 2 is provided with An air intake pipe with a connection block 36 is connected to the air chamber 33, and a precise air pressure regulating valve 37 is arranged on the air intake pipe. The liquid outlet 32 and the gas outlet 31 are on the same axis, and the aperture of the gas outlet 31 is larger than the aperture of the liquid outlet 32. As preferably, the aperture of the gas outlet 31 is 0.6mm-1mm, and the aperture of the liquid outlet 32 The hole diameter is 0.1mm-0.6mm. The liquid jet head has a tapered tip in the air chamber 33, through which an airflow passage 35 that gathers to the air outlet 31 is formed inside the air chamber 33, and the airflow blown out by the airflow passage 35 wraps around the liquid outlet 32, Thus, under the shear force of high-speed air flow, jet stretching is realized to obtain nanofibers.
一种纳米纤维三维支架制备方法，包括以下步骤： A method for preparing a nanofiber three-dimensional scaffold, comprising the following steps:
S10.纺丝溶液的制备，将生物降解材料溶于溶剂中，密闭搅拌，得到纺丝溶液；将所得纺丝溶液置于供液装置1中，启动供液装置1，纺丝溶液由进液管进入同轴喷头3的喷液头34； S10. Preparation of spinning solution, dissolving the biodegradable material in a solvent, and stirring in an airtight manner to obtain a spinning solution; placing the obtained spinning solution in the liquid supply device 1, starting the liquid supply device 1, and the spinning solution is fed into the liquid The pipe enters the liquid spray head 34 of the coaxial spray head 3;
S20.启动接收装置4，接收装置4中传动装置带动转轴旋转，支撑臂随转轴转动时可形成碗状回转面； S20. Start the receiving device 4, the transmission device in the receiving device 4 drives the rotating shaft to rotate, and the support arm can form a bowl-shaped rotating surface when rotating with the rotating shaft;
S30.同轴喷头3上的喷液头34伸入所述气室33内，所述喷液头34在气室33内的一端设有出液口32，所述气室33上在出液口32的对侧设有出气口31，打开供气装置2，调节精密气压调节阀37，气体经过精密气压调节阀37进行气压调节后，流经进气管进入同轴喷头3的气室33，最终从出气口31处喷出，并产生将喷液头34的出液口32内的溶液吹气拉伸并在同轴喷头3与接收装置4间形成对射流进行辅助拉伸的气流，在气流的持续作用下，实现射流拉伸，获得纳米纤维； S30. The liquid spray head 34 on the coaxial spray head 3 extends into the air chamber 33, and one end of the liquid spray head 34 in the air chamber 33 is provided with a liquid outlet 32. An air outlet 31 is provided on the opposite side of the port 32. Open the air supply device 2 and adjust the precision air pressure regulating valve 37. After the air pressure is adjusted by the precision air pressure regulating valve 37, the gas flows through the air intake pipe and enters the air chamber 33 of the coaxial nozzle 3. Finally, it is ejected from the air outlet 31, and the solution in the liquid outlet 32 of the liquid ejection head 34 is blown and stretched to form an airflow that assists the stretching of the jet between the coaxial nozzle 3 and the receiving device 4. Under the continuous action of airflow, jet stretching is realized to obtain nanofibers;
S40.在气流推力作用下，纳米纤维往接收装置4方向沉积，同时，气流从接收装置4的侧端导出，并辅助纤维在接收装置上沉积成为三维支架结构。 S40. Under the thrust of the airflow, the nanofibers are deposited in the direction of the receiving device 4, and at the same time, the airflow is led out from the side of the receiving device 4, and assists the deposition of the fibers on the receiving device to form a three-dimensional scaffold structure.
实施例： Example:
1.纺丝溶液制备，称取1200毫克PLLA（分子量=20万道尔顿），溶于20ml（9:1,CH2Cl2/DMF,v/v）溶剂中，制得6%PLLA溶液，用封口膜封口，磁力搅拌4小时，待用。 1. Preparation of spinning solution, weigh 1200 mg of PLLA (molecular weight = 200,000 Daltons), dissolve it in 20 ml (9:1, CH 2 Cl 2 /DMF, v/v) solvent, and prepare a 6% PLLA solution , sealed with a parafilm, magnetically stirred for 4 hours, and set aside.
2.调节微量注射泵参数，容量10毫升，推进速度5毫升/小时，运行。 2. Adjust the parameters of the micro-injection pump, the volume is 10 ml, the propulsion speed is 5 ml/hour, and it is running.
3.调节传动机构，使爪结构在电机或其他传动机构带动下进行旋转，可形成虚拟的碗状回转面； 3. Adjust the transmission mechanism so that the claw structure rotates under the drive of the motor or other transmission mechanisms to form a virtual bowl-shaped rotary surface;
4.打开供气装置，调节精密气压调节阀至0.75MPa，流经进气管进入同轴喷头的气室，最终从出气口处喷出； 4. Open the air supply device, adjust the precision air pressure regulating valve to 0.75MPa, flow through the air inlet pipe into the air chamber of the coaxial nozzle, and finally spray out from the air outlet;
5.在高速气流的剪力作用下，可以使液滴拉伸形成射流，并在气流的持续作用下，实现射流拉伸，获得PLLA纳米纤维； 5. Under the shear force of high-speed airflow, the droplet can be stretched to form a jet, and under the continuous action of the airflow, the jet stretch can be realized to obtain PLLA nanofibers;
6.在高速气流推力作用下，PLLA纳米纤维往接收装置方向沉积，同时，气流从接收装置形成的碗状回转面的侧端导出，并辅助纤维在收集器上沉积成为PLLA纳米纤维三维支架结构。 6. Under the thrust of the high-speed airflow, PLLA nanofibers are deposited towards the receiving device. At the same time, the airflow is guided from the side end of the bowl-shaped rotating surface formed by the receiving device, and assists the deposition of fibers on the collector to form a three-dimensional scaffold structure of PLLA nanofibers. .
本发明与现有技术相比，具有如下有益效果： Compared with the prior art, the present invention has the following beneficial effects:
本发明中纳米纤维三维支架制备装置结构简单，能促使三维结构的气纺纳米纤维支架在高速气纺的过程中直接形成。 The preparation device of the nanofiber three-dimensional support in the present invention has a simple structure, and can promote the direct formation of the three-dimensional air-spun nanofiber support in the high-speed air-spinning process.
本发明中纳米纤维三维支架制备方法工艺简单，成本较低，适于用不同的纺丝材料制备不同力学强度、生物相容性及降解性能的三维纤维支架，并且可以通过气流纺丝的方法直接制备大量微纳米纤维支架； The preparation method of the nanofiber three-dimensional scaffold in the present invention has simple process and low cost, and is suitable for preparing three-dimensional fiber scaffolds with different mechanical strength, biocompatibility and degradation performance with different spinning materials, and can be directly processed by air spinning. Prepare a large number of micro-nano fiber scaffolds;
通过本发明所述的技术方案无需高压电场，在高速气流的剪力作用下，即可以实现射流拉伸，获得纳米纤维，安全可靠，材料兼容性好，尤其适用于导电性差或具有生物活性的材料。同时，由于生产原理有本质创新，相比静电纺丝，无残余电荷积累和电场积累等因素造成的厚度限制，可获得极高厚度的三维支架结构。而且，在高速气流的作用下，生产效率极大地提高，是传统静电纺丝的10-40倍。 The technical solution of the present invention does not require a high-voltage electric field, and under the shear force of the high-speed airflow, jet stretching can be realized to obtain nanofibers, which are safe and reliable, and have good material compatibility, especially suitable for materials with poor conductivity or biological activity. Material. At the same time, due to the essential innovation of the production principle, compared with electrospinning, there is no thickness limitation caused by factors such as residual charge accumulation and electric field accumulation, and a three-dimensional scaffold structure with extremely high thickness can be obtained. Moreover, under the action of high-speed airflow, the production efficiency is greatly improved, which is 10-40 times that of traditional electrospinning.
所述接收装置，根据线动成面的思想，创新地采用旋转的敞开式收集器，相比传统平板收集器或者封闭式收集器，可有效地使高速气流通过，从而避免产生反冲气流，影响纤维沉积。相反地，高速气流将帮助纤维在收集器上沉积成为三维支架结构。传统的静电纺丝是在电场的作用下，逐层被接地收集板吸引堆积，支架的纤维排列紧密、纤维之间的空隙过小，使细胞难以长入，无法构建出令人满意的三维组织。而采用气流拉升形成纤维并辅助沉积，可获得疏松、低密度的结构，更有利于细胞生长。 The receiving device innovatively adopts a rotating open collector based on the idea of linear movement into a surface. Compared with traditional flat collectors or closed collectors, the receiving device can effectively allow high-speed airflow to pass through, thereby avoiding the generation of recoil airflow. Affects fiber deposition. Conversely, the high-velocity gas flow will help deposit the fibers into a three-dimensional scaffold structure on the collector. Traditional electrospinning is under the action of an electric field, which is attracted and accumulated layer by layer by a grounded collecting plate. The fibers of the scaffold are tightly arranged, and the gaps between the fibers are too small, making it difficult for cells to grow in, and it is impossible to construct a satisfactory three-dimensional tissue. . However, the use of airflow to form fibers and assist in deposition can obtain a loose, low-density structure, which is more conducive to cell growth.
通过本发明获得的三维纤维支架具有优越的蓬松结构，同时还具有良好的生物相容性、机械性能和生物可降解性能，为细胞外基质建立提供良好的环境，有利于细胞的繁殖和组织的愈合，将推动组织工程领域在模拟体内环境发展。 The three-dimensional fiber scaffold obtained by the present invention has a superior fluffy structure, and also has good biocompatibility, mechanical properties and biodegradability, and provides a good environment for the establishment of extracellular matrix, which is beneficial to the proliferation of cells and the formation of tissues. Healing will advance the field of tissue engineering in simulating in vivo environments.
当然，本发明创造并不局限于上述实施方式，熟悉本领域的技术人员在不违背本发明精神的前提下还可作出等同变形或替换，这些等同的变型或替换均包含在本申请权利要求所限定的范围内。 Of course, the present invention is not limited to the above-mentioned embodiments. Those skilled in the art can also make equivalent modifications or replacements without violating the spirit of the present invention. These equivalent modifications or replacements are all included in the claims of this application. within a limited range.
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