CN105273980B - The DNA sequencer that a kind of reaction warehouse is adaptively adjusted - Google Patents
The DNA sequencer that a kind of reaction warehouse is adaptively adjusted Download PDFInfo
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- CN105273980B CN105273980B CN201510716595.5A CN201510716595A CN105273980B CN 105273980 B CN105273980 B CN 105273980B CN 201510716595 A CN201510716595 A CN 201510716595A CN 105273980 B CN105273980 B CN 105273980B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
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Abstract
The present invention relates to the DNA sequencer that a kind of reaction warehouse is adaptively adjusted, reaction warehouse component includes the door frame being vertically arranged being arranged on described platform component, turnable reaction warehouse component is provided with the top in the outside of door frame, turnable basement is fixed on the middle part of door frame and can rotated around described turnable basement；Reaction chip component is installed on the inside of the centre of described turnable warehouse, it may be reversed and be provided with adaptive adjustment part between reaction warehouse and reaction warehouse base, adaptive adjustment part passes through adaptive spring mounting hole, and the end contact of end spring and reaction warehouse base.The DNA sequencer that the reaction warehouse of the present invention is adaptively adjusted, when being fed using CCD camera, it can be adjusted by the way of movable, now, adaptive adjustment part can change the position of reaction chip component according to the change in location of camera, ensure that there is higher accuracy of detection, simultaneously, it is to avoid system is caused to damage.
Description
Technical field
The present invention relates to DNA sequencing field, the DNA sequencer that auditor and a kind of reaction warehouse are adaptively adjusted especially is related to.
Background technology
During DNA sequencing of the prior art, chemically reacted on sequence testing chip, produce visible ray, pass through CCD
Optical signal produced by (Charge Couple Device) cameras capture sequencing reaction, you can the sequencing letter required for obtaining
Breath.
In test, reaction chip is installed with reaction warehouse component, when taking pictures, the shifting that CCD camera passes through downside
Dynamic structure is moved and moves closer to reaction chip, and in CCD camera and reaction warehouse component touch, punching can be caused to reaction warehouse component
Hit, accordingly, it would be desirable to which reaction warehouse component has the performance adaptively adjusted.
In measuring technology, reaction warehouse with enough stability and should can be grasped as the main carriers of test reaction
The property made, can be according to actual reaction condition, convenient adjustment.
In view of drawbacks described above, creator of the present invention obtains this creation finally by prolonged research and practice.
The content of the invention
It is an object of the invention to provide the DNA sequencer that a kind of reaction warehouse is adaptively adjusted, to overcome above-mentioned technology
Defect.
To achieve the above object, the present invention provides a kind of adaptive reaction warehouse of DNA sequencer, the adaptive reaction warehouse bag
Reaction warehouse component is included, the reaction warehouse component includes the door frame being vertically arranged being arranged on platform component and its in door frame
Outside top set turnable reaction warehouse component；Turnable basement is connected with the lower end that reaction warehouse component may be reversed,
And can be rotated around described turnable basement, described turnable basement is fixed on the middle part of door frame；
The turnable reaction warehouse component includes reaction warehouse may be reversed；Inner side in the centre of described turnable reaction warehouse
Reaction chip component is installed, accordingly, the first chamber for accommodating reaction chip component is provided with the centre that reaction warehouse may be reversed
Body；
Described reaction chip component includes reaction chip mounting seat, reaction warehouse base assembly and by reaction warehouse base group
Part and the locking of reaction chip mounting seat, the shading positioning component opened, reaction chip mounting seat can be with reaction warehouse base assembly
Rotate against；
Reaction chip is installed in the center section of reaction chip mounting seat, cover plate is provided with reaction chip；Anti-
Answer the both sides of chip that chute pressing plate is installed, reaction chip to be fixed；Set in the center section of reaction warehouse base
There is temperature sensor mounting groove；
The surrounding of temperature sensor mounting groove is provided with four guide rod mounting holes, is provided with adaptively in its upper and lower sides
Spring mounting hole；It is also equipped with being oriented to rod aperture on the cover board；It may be reversed and be provided between reaction warehouse and reaction warehouse base adaptively
Adjustment part, adaptive adjustment part passes through adaptive spring mounting hole, and the end contact of end spring and reaction warehouse base；
On reaction warehouse base set be oriented to rod aperture, may be reversed reaction warehouse on set guide rod mounting hole, guide rod according to
It is secondary to pass through holes；
Reaction chip component, which is arranged on, may be reversed in reaction warehouse, install guide rod and adaptive adjustment part, and encapsulate
Good back shroud；Wherein, the back shroud is located at the center section that reaction warehouse may be reversed, and is set in the center section of back shroud
There is adaptive adjustment part.
Further, two rows are provided with the middle of the inner side of described back shroud raised line is installed, the centre of raised line is being installed
The first installation base and the second installation base are provided with, screwed hole is offered on installation base, adaptive adjustment part is arranged on
In the screwed hole.
Further, the connection end of described adaptive adjustment part and back shroud is the end of thread, the company with reaction warehouse component
It is the polished rod for being provided with spring to connect end, and nut is provided between the ends and is positioned, after installation is complete, nut is close to installation
Boss；
It is passage between two described row's raised lines, enables to Reagent Tube to pass through；Be additionally provided with back shroud with it is convex
The vertical stiffener plate of bar；The upper surface of raised line and boss is plane.
Further, it is provided with first boss in the bottom of the first described cavity, first boss is to carry described
Reaction chip component, four guide rod mounting holes are provided with first boss, and guide rod mounting hole, which is passed through, may be reversed reaction warehouse
Two ends, reaction chip component, reaction chip component is connected with turnable reaction warehouse；
Second boss is provided with the upper end of described first boss, second boss is to position described support member；
Described second boss upper end is provided with locating surface, and locking screw nail is provided with locating surface；
The described reaction chip component of first cavity body structure positioning, also, reaction chip component is in normal work, it is impossible to
Enough rotations, are merely able to move along guide rod.
Beneficial effects of the present invention are compared with prior art：The DNA sequencer that the reaction warehouse of the present invention is adaptively adjusted,
,, can be using front and rear shifting when adjusting camera position when camera is with reaction chip component touch when being fed using CCD camera
Dynamic mode is adjusted, now, and adaptive adjustment part can change the position of reaction chip component according to the change in location of camera, protect
Card has higher accuracy of detection, simultaneously, it is to avoid system is caused to damage.
Brief description of the drawings
Fig. 1 records the overall structure diagram of subsystem for the DNA sequencer reaction of the present invention；
Fig. 2 is the first dimensional structure diagram of the reaction warehouse component of the present invention；
Fig. 3 is the second dimensional structure diagram of the reaction warehouse component of the present invention；
Fig. 4 is the positive structure diagram of the reaction warehouse component of the present invention；
Fig. 5 a are the dimensional structure diagram of the turnable reaction warehouse component of the present invention；
Fig. 5 b are the first dimensional structure diagram of the turnable reaction warehouse of the present invention；
Fig. 5 c are the second dimensional structure diagram of the turnable reaction warehouse of the present invention；
Fig. 5 d are the first dimensional structure diagram of the locating piece of the present invention；
Fig. 5 e are the second dimensional structure diagram of the locating piece of the present invention；
Fig. 6 a are the structural representation of the electronic lock of the present invention；
Fig. 6 b are the structural representation of the electronics lock of the present invention；
Fig. 6 c are the cross section structure diagram of the electronic lock of the present invention；
Cross section structure diagrams of the Fig. 7 for turnable reaction warehouse component of the invention along first lug；
Fig. 8 is the 3rd dimensional structure diagram of the turnable reaction warehouse of the present invention；
Fig. 9 a are the structural representation of the reaction chip component of the present invention；
Fig. 9 b are the structural representation of the reaction chip mounting seat of the present invention；
Fig. 9 c are the structural representation of the medial surface of the reaction warehouse base of the present invention；
Fig. 9 d are the structural representation of the lateral surface of the reaction warehouse base of the present invention；
Fig. 9 e are the structural representation of the temperature control component cover plate of the present invention；
Figure 10 a are the structural representation of the shading positioning component of the present invention；
Figure 10 b are the first structure schematic diagram of the slidably positioning and locking block of the present invention；
Figure 10 c are the second structural representation of the slidably positioning and locking block of the present invention；
Figure 10 d are the structural representation of the turnable basement component of the present invention；
Figure 10 e are the structural representation of the spring assembly of the present invention；
Figure 11 a are the cross section structure diagram along liquid inlet and outlet of the reaction chip component of the present invention；
Cross section structure diagrams of Figure 11 b for turnable reaction warehouse component of the invention along adaptive adjustment part；
Cross section structure diagrams of Figure 11 c for turnable reaction warehouse component of the invention along guide rod；
Figure 12 for the present invention turnable reaction warehouse component along the A-A in Fig. 4 to cross section structure diagram；
Figure 13 a are the cross section structure diagram of the back shroud of the present invention；
Figure 13 b are the dimensional structure diagram of the bonnet board component of the present invention；
Figure 13 c are the dimensional structure diagram of the back shroud of the present invention；
Figure 14 a are the structural representation of the sealing ring of the present invention；
Figure 14 b are sealing ring cross section structure diagram of the invention；
Figure 14 c observe the use state schematic diagram of the sealing ring of right side reaction chip for the present invention.
Embodiment
Below in conjunction with accompanying drawing, the forgoing and additional technical features and advantages are described in more detail.
Refer to shown in Fig. 1, it records the overall structure diagram of subsystem for the DNA sequencer reaction of the present invention, this
In inventive embodiments reaction record subsystem include reaction warehouse component 12, motion platform component 14, motion control unit 15,
CCD camera 101 and platform support component 13, wherein,
Described reaction warehouse component 12, setting reaction chip component in it, in the presence of control system, reaction solution enters
Chemically reacted in reaction chip, produce visible ray；Described motion platform component 14, CCD camera is fed into suitably by it
Picture-taking position, collection of taking pictures is carried out to the visible optical information that is produced in reaction chip；Described platform component 13, it is propped up
The described reaction warehouse component 12 of support, motion platform component 14；Described motion control unit, it is to CCD camera and reaction core
The operation of piece is controlled in real time.
In embodiments of the present invention, described reaction warehouse component 12 can be installed, fixed and adjust described reaction chip,
Ensure that reaction chip is reacted in stable environment, also, reaction chip conveniently can take out and lock.
Refer to shown in Fig. 2-4, described reaction warehouse component 12 includes being arranged on described platform component 13
The door frame 121 being vertically arranged, turnable reaction warehouse component 124 is provided with the top in the outside of door frame 121, and reaction is may be reversed
The lower end of storehouse component 124 is connected with turnable basement 122, and turnable basement 122 is fixed on the middle part of door frame 121, may be reversed anti-
Storehouse component 124 is answered to be rotated around described turnable basement 122, to take out or be put into reaction chip.
Electronics lock set 125 is additionally provided with the left of the upper end of described door frame 121, only in electronics lock set 125
During opening, turnable reaction warehouse component 124 can be rotated, reaction chip is operated.In embodiments of the present invention, if
Door frame 121, support reaction chip and dependency structure are equipped with, the stability of system is substantially increased.
Reaction warehouse component 124, which may be reversed, to be included reaction warehouse may be reversed, and is pacified in the center section of described turnable reaction warehouse
Equipped with back shroud 1242, to encapsulate Reagent Tube, and reaction warehouse component is adjusted by adaptive adjustment part 1751 thereon and can
Overturn the position of reaction warehouse.
The bottom that reaction warehouse may be reversed is provided with the first rotating part, and it includes the first accommodating chamber 1243, can be turned over to accommodate
Turn base 122, the first accommodating chamber 1243 is arranged on the centre position of bottom；It is symmetrically arranged first in the first accommodating chamber both sides
Lug 1244, through hole 1246 is provided with the centre of first lug 1244；To be connected with described turnable basement 122.Can
Base is overturn to rotate in the first accommodating chamber 1243 relative to turnable reaction warehouse, when turnable reaction warehouse turns to horizontal level,
Reaction chip component is operated；With higher stability, while ensureing that the transmission of reagent and data is unimpeded.
Refer to shown in Fig. 5 a-5c, reaction chip component be installed on the inside of the centre of described turnable reaction warehouse,
Accordingly, the first cavity 1785 for accommodating reaction chip component is provided with the centre that reaction warehouse may be reversed；In the first cavity
1785 bottom is provided with first boss 1784, and first boss 1784 is convex first to carry described reaction chip component
Platform 1784 is provided with four guide rod mounting holes 1786, and guide rod mounting hole 1786 passes through the two ends that reaction warehouse may be reversed, reaction
Chip assembly, reaction chip component is connected with turnable reaction warehouse；Is provided with the upper end of described first boss 1784
Two boss 1783, second boss 1784 is to position described support member；It is fixed to be provided with the described upper end of second boss 1784
Plane 1782, is provided with locking screw nail 1787 on locating surface 1782.The described reaction core of above-mentioned first cavity body structure positioning
Piece component, also, reaction chip component is in normal work, it is impossible to and rotate, be merely able to move along guide rod.
Reagent Tube mounting hole 1788 is provided with the upper surface of the first accommodating chamber 1243 of described turnable reaction warehouse
With electric wire mounting hole, being provided with to pass through in pipe clamp 03, pipe clamp 03 in Reagent Tube mounting hole 1788 has Reagent Tube 02 and electric wire 01.
The dead bolt for being additionally provided with the dead bolt 1252 in electronics lock set 125 in the sidepiece of described turnable reaction warehouse is installed
Hole；In the presence of locking member, reaction warehouse may be reversed and is fixed together with door frame 121.
Incorporated by reference to shown in Fig. 6 a and 6b, electronics lock set 125 of the invention includes electronics lock and inductive switch 1251, institute
The inductive switch stated is arranged on electronics lock, and electronics lock is arranged on the sidepiece of turnable reaction warehouse, in the embodiment of the present invention
In, electronics lock includes sidepiece installing plate 1843, and mounting hole 1844 is offered on sidepiece installing plate 1483；Electronic lock is made to pass through
Mounting hole 1844 and mounting screw are fixed on turnable reaction warehouse.It is provided with the described side of sidepiece installing plate 1483 pair
The first framework 1841 and second framework 1842 claimed, installation inductive switch 1251 is left in the centre of the first framework and second framework
Space.
In embodiments of the present invention, described inductive switch 1251 includes being arranged on the framework 1255 of outside, framework 1255
One side on be provided with connection screw thread hole 1256, it is fixed with described electronics lock by attachment screw 1254；Described
Attachment screw 1254 is by the framework 1841 of lug 1258 and first, the second framework 1842 on side, to increase electronics lock
Bonding strength.Control circuit 1252 is also associated with described inductive switch 1251, to be carried out to inductive switch 1251
Control.
Refer to shown in Fig. 6 c, described electronics lock set 125 is arranged on the sidepiece that reaction warehouse and door frame 121 may be reversed,
Dead bolt 1253 enters after stretching out to be arranged in the dead bolt hole 1247 of turnable reaction warehouse sidepiece, realizes locking；When dead bolt 1253 exists
In the presence of electro photoluminescence, when being retracted from dead bolt hole 1247 is interior, described turnable reaction warehouse can be rotated.
The upper end in the outside of described turnable reaction warehouse is provided with pressing piece 1241, is only passing through pressing piece 1241
Pressing, which is set, can rotate described turnable reaction warehouse.Refer to shown in Figure 12, it is in embodiments of the present invention, described
Pressing piece 1241 is that one end is provided with the structure that opening 1730, one end are provided with hook, described hook and the upper terminal block of door frame
Plate is connected, when pressing piece 1241 is by downward active force, and hook departs from door frame baffle plate, and turnable reaction warehouse can be relative
Door frame rotates.
In embodiments of the present invention, described pressing piece 1241 is arranged in a positioning block 1762, under locating piece 1762
End is provided with spring 1761, and spring is arranged in spring base 1763, and spring base 1763 is threadably mounted in turnable reaction warehouse
Between the upper surface of through hole 1785 spring base hole 1781 in.
Incorporated by reference to shown in Fig. 5 b-5d, locating piece 1762 is arranged on the locating piece installation cavity 1791 that reaction warehouse upper end may be reversed
In, positioning step 1790 is provided with cavity.Locating piece 1762 in the embodiment of the present invention includes upper through hole 1794 and lower logical
Hole 1797, one of both passes through described pressing piece 1241；Locating and machining hole 1796 is set in the side of locating piece and installed
Hole 1795.Spring eye is provided with the lower end for the through hole for installing pressing piece 1241, spring passes through the hole and the bottom of pressing piece 1241
Portion is contacted.
The embodiment of the present invention sets electronics lock set and pressing piece, and two are carried out to reaction warehouse component and door frame may be reversed
Secondary locking, is respectively adopted automatically controlled and mechanical structure mode, it is ensured that reaction chip is run in security context, it is to avoid maloperation.
Refer to shown in Fig. 2 and 7, the turnable basement 122 in the embodiment of the present invention is with two the second lugs 1221
Connect base, the second lug 1221 is symmetricly set on a side 1223 of base, is provided with through hole 1224 in the middle of it,
It is co-axially mounted with the through hole 1246 of setting in the middle of the first lug 1244 on described turnable reaction warehouse, also, second convex
Ear 1221 is arranged on the inner side of first lug 1244.Turnable basement 122 is the intermediate connector set, and reaction warehouse may be reversed and leads to
This is crossed the realization of connecting seat 122 upset may be reversed and positions, it is easy to operate.
Incorporated by reference to shown in Fig. 2, in embodiments of the present invention, door frame 121 is the structure that one side has sidepiece baffle plate 1211,
It is integrally formed, and the both sides of described turnable basement 122 are arranged in sidepiece baffle plate 1211 and contacted with sidepiece baffle plate 1211
Positioning.
Described first lug 1224 with the second lug 1221 after being co-axially mounted, and end is upper with turnable basement 122
End in contact, also, may be reversed reaction warehouse sidepiece contacted with the medial surface of sidepiece baffle plate 1211 of door frame 121, it is ensured that may be reversed instead
Ying Cangyu turnable basements 122 are located just in sidepiece baffle plate 1211.
The receiving hole passed through for reaction chip component is additionally provided with door frame 121.The use of door frame, be not only able to so that
The Stability Analysis of Structures of reaction chip component, moreover, also causing reaction warehouse and turnable basement compact conformation may be reversed, is easy to operation；
The positioning of CCD camera and reaction chip is more accurate.
The first mounting hole 1210 is provided with the bottom of door frame 121, to be connected with motion platform component 14；In door
The second mounting hole being connected with turnable basement sidepiece is additionally provided with the middle part of frame.
Incorporated by reference to shown in Fig. 7, in embodiments of the present invention, in order to save structure space and ensure the steady of reagent delivery
It is fixed, it is provided with reagent in the through hole of the lug 1221 of through hole 1246 and second set in the middle of described first lug 1244
The fixation kit of pipe first, it includes the pipe joint 1265, the first packing ring 1264, the first rotary shaft being arranged in the lug of left side
1261, and be arranged on right side lug in reagent pipe joint 1266, second packing ring 1267, the second rotary shaft 1269, wherein, institute
The first packing ring 1264 and second packing ring 1267 stated are arranged between turnable reaction warehouse and turnable basement, in the present embodiment
In, the diameter of the through hole 1246 set in the middle of described first lug 1244 is less than the straight of the through hole of the second lug 1221
Footpath, both medial ends formed to packing ring position positioning rib, after two packing rings are installed, inside diameter of gasket just with
The diameter of the through hole of second lug 1221 is identical.Positioning rib and packing ring are set in embodiments of the present invention, it is ensured that can be turned over
Turn gapless between reaction warehouse and turnable basement, both stability after combining are good, will not be rocked because there is gap,
Also, packing ring adds both contacts area so that stress reduction between the two, extension device service life.
The first rotation is installed on the inside of the inner side of the through hole 1246 set in the middle of first lug 1244 and packing ring
The rotary shaft 1269 of axle 1261 and second, the structure of two rotary shafts is identical, is the quill shaft with positioning convex ring；In rotary shaft
One end set positioning convex ring, connection screw thread hole is provided with, to the lug connecting portion with described turnable reaction warehouse
Portion 1263 is connected, accordingly, and the positioning recess for positioning the positioning convex ring is provided with the end of described through hole 1246.
It is recess in the centre at the both ends of rotary shaft 1269, inner side recess is used to and described pipe joint 1265, reagent pipe joint
1266 install positioning.Live wire and Reagent Tube 02 are respectively mounted in pipe joint 1265, reagent pipe joint 1266, it is distinguished
Lead in reaction chip component.Due to setting the component that can mutually overturn in the embodiment of the present invention, in upset, to avoid to pipe
Part is impacted, and Reagent Tube is installed in through-holes, substantially increases the security performance of Reagent Tube.
Refer to shown in Fig. 8, in embodiments of the present invention, described reaction chip component is arranged on described turnable anti-
Answer the inner side in storehouse.The observation portion 1724 contacted with CCD camera is provided with the inside of reaction chip component, is provided with transparent
Baffle plate, to be taken pictures to course of reaction.
Refer to shown in Fig. 9 a, reaction chip component of the invention includes reaction chip mounting seat 127, reaction warehouse base group
Part 126 and the shading positioning component 128 that reaction warehouse base assembly 126 and reaction chip mounting seat 127 are locked, opened；For
Easily reaction chip can be taken out and be closed, reaction chip mounting seat 127 can be relative with reaction warehouse base assembly 126
Rotation.
Refer to shown in Fig. 9 b, it is the structural representation of the reaction chip mounting seat of the present invention, is installed in reaction chip
The center section of seat 127 is provided with reaction chip, and cover plate 1277 is provided with reaction chip；Installed in the both sides of reaction chip
There is chute pressing plate 1279, reaction chip to be fixed.A side portion of reaction chip mounting seat 127 is provided with installation
Seat supports component, it is used in taking-up or chip placement, the described reaction chip mounting seat of support；It includes torsional-spring supported portion
1273, it is arranged in mounting seat, the described torsion spring to support, described torsional-spring supported portion 1273 carries protruding end 1274 for one
Recess, in the reaction chip mounting seat 127 described in rotation, protruding end 1274 is contacted with 1261 at first, in the present embodiment,
Described protruding end 1274 is arranged on the middle part of recess and the edge of chip installation position；Using the structure of mounting seat in itself
Reaction chip mounting seat is supported, better reliability, it is ensured that chip can be safely removed and placed.
The drawing that described mounting seat support component also includes torsion spring 1272, is connected with torsion spring 1272 and is arranged on torsion spring two ends
Mandrel 1271, described tension axle 1271 is arranged on lug boss 1275, when starting reaction chip mounting seat, described torsion spring
1272 support mounting seats, and the positioning of protruding end 1274 is finally relied on, realize support.
The first rotary shaft hole 1276 is additionally provided with the end of described lug boss 1275, to reaction warehouse base 1261
Connection.Locating slot 1278 is additionally provided with the sidepiece of reaction chip mounting seat.
Refer to shown in Fig. 9 c, it is the structural representation of the medial surface of the reaction warehouse base of the present invention, in reaction warehouse
Reaction pond body 1266 is provided with medial surface, reaction tank 1267 is set in reaction pond body 1266 center section, in reaction tank 1267
Surrounding 1706 be provided with sealing ring, prevent reaction reagent from leaking.One end of reaction warehouse base 1261 is provided with raised line 1705,
To be positioned with the cover plate after installation.The torsional-spring supported structure corresponding with reaction chip mounting seat is provided with the other end, its
Including symmetrically arranged two protruding ends 1268, the second rotary shaft hole 1269 is being provided with, itself and the first rotary shaft hole
1276 is coaxial, and rotary shaft sequentially passes through holes；The region 1260 and localization region for torsion spring activity are additionally provided with the end
1707, it is provided with the region the screwed hole 1712 for reacting Hatch Board will be installed.
Refer to shown in Fig. 9 d, it is the structural representation of the lateral surface of the reaction warehouse base of the present invention, at reaction warehouse bottom
The center section of the lateral surface of seat is provided with temperature control component, and temperature sensor installation is provided with the center section of reaction warehouse base
Groove 1262, heating wire mounting groove 1704 is provided with the bottom of mounting groove 1262, and heating wire and temperature sensor are placed respectively.
A positioning boss 1703 is additionally provided with mounting groove, it when being installed with temperature control component cover plate 1263 to position.Such as Fig. 9 e institutes
Show, positioning recess 1702 corresponding with positioning boss 1703 is provided with temperature control component cover plate 1263.
The surrounding of temperature sensor mounting groove 1262 is provided with four guide rod mounting holes 1742, is provided with upper and lower sides
Adaptive spring mounting hole 1741；It is also equipped with being oriented to rod aperture 1701 on cover plate 1263.
Shading positioning component mounting groove 1265 is additionally provided with reaction warehouse base, to install described shading positioning group
Part 128, is provided with installation base 1744 in it.
Refer to shown in Figure 10 a, it is the structural representation of the shading positioning component of the present invention, and it includes slidably positioning
Latch segment 1281 and turnable basement component 1283, wherein, it is provided with locking jaw on described slidably positioning and locking block 1281
1282, after installation is complete, the upper surface of described slidably positioning and locking block 1281 and the inner side of reaction chip mounting seat 127
Face is contacted, and locking jaw 1282 is hooked on the outboard end of reaction chip mounting seat 127；Described turnable basement component 1283 is pacified
Mounted in the medial end of reaction warehouse base, screwed hole 1830 is provided with.After installation is complete, described shading positioning component
128 are fixed together with reaction warehouse base, when opening reaction chip mounting seat, outwards press slidably positioning and locking block
1281, under the action of the spring, slidably positioning and locking block 1281 is outward-dipping, and the reaction warehouse mounting seat of locking jaw more than 1282 takes off
From in the presence of the torsion spring of reaction warehouse mounting seat end, the upset of reaction warehouse mounting seat can be changed to reaction chip.
Incorporated by reference to shown in Figure 10 b, described slidably positioning and locking block 1281 includes being arranged on the pressing plate 1823 on top
And the elastic locking component of bottom homonymy, convex joint face 1821 under being in the center section of pressing plate 1823, its with it is described
Turnable basement component 1283 coordinate.Incorporated by reference to shown in Figure 10 c, described elastic locking component includes symmetrically arranged first
Spring base 1822, second spring seat 1828, the first recess 1827 being arranged between two spring bases；Divide in two spring bases
It is not provided with spring assembly, spring assembly includes upper spring card slot 1281, the lower spring neck 1283 shown in Figure 10 f, up and down two
Individual spring card slot surrounds spring eye 1771, and spring eye is shoulder hole, is provided with spring 1820 in it；Wherein, in upper spring card slot
External part is provided with 1281, sets hook claw thereon, be connected to the end with described pressing plate 1823.
In embodiments of the present invention, spring base and the first recess are formed by the isolation of continuous vertical baffle 1826, and two
Side sets vertical rib 1829；Elastic locking component be arranged on on integrally formed first flat board 1825 of pressing plate, its with by
Pressing plate 1823 is vertically arranged.
Refer to shown in Figure 10 d, the slidably positioning and locking block 1281 in the embodiment of the present invention and turnable basement component
1283 can symmetrically coordinate, and the first projection 1832 is provided with the centre of turnable basement, and it is fastened on the first recess 1827
Together；The space of vertical baffle 1826 can be accommodated by being provided with the both sides of the first projection 1832；Accordingly, in 1832 liang of the first projection
Side is symmetrically arranged with the second recess 1833 of the spring base described in accommodating, and the second recess is surrounded by continuous vertical edge 1834；
Said structure is arranged on integrally formed second flat board 1831；When in use, spring assembly is pressed against in spring base, by cunning
Dynamic positioning and locking block 1281 is snapped together with turnable basement component 1283.The embodiment of the present invention, sets shading to position
Component, is not only able to stable locking reaction chip, and when changing reaction chip, it is simple to operate, it need to only press slip fixed
Position locking block 1281 is opened, and without dismounting reaction chip mounting seat, saves the time.
Refer to shown in Figure 11 a and 11b, the middle part of reaction warehouse base is provided with electrical heating wire 1754, it is set
In the heating wire mounting groove 1704 of base；Temperature biography is provided with the temperature sensor mounting groove 1262 on electrical heating wire top
Sensor 1720 and control assembly, to detect the temperature of reaction tank and be adjusted according to the threshold value of setting；In temperature sensor
1720 and control assembly 1753 also pour and be inoculated with seal 1263, its be encapsulating resin.
Reagent Tube is respectively arranged with the two ends of reaction tank screwed hole 1721 is installed, to be connected with Reagent Tube；In reagent
The end of pipe installation screwed hole 1721 is provided with the two ends of inlet 1723 and liquid outlet 1713, liquid inlet and outlet and reaction tank
Communicate, to cycle detection reagent.
The avris of the interior grooves of reaction tank is provided with sealing ring 1713.It is provided with the centre of reaction chip mounting seat
Reaction chip 04, reagent flows through reaction chip and reacted.
In embodiments of the present invention, Reagent Tube 02 is by the Reagent Tube mounting hole 1788 of turnable reaction warehouse, and being arranged on can
The centre of upset reaction warehouse is provided with the first cavity 1785 for accommodating reagent pipeline, final coupled reaction pond body, reagent cartridge warp
The sealing of various cavitys is crossed, stability is high, is hardly damaged.
Back shroud 1242 is fixed with rear side of reaction warehouse, the centre of back shroud 1242 is provided with adaptive adjustment part
1751, refer to shown in Figure 13 a-13c, two rows are provided with the middle of the inner side of back shroud 1242 raised line 1809 is installed, installing
The centre of raised line 1809 is provided with the first installation base 1812 and the second installation base 1811, and screw thread is offered on installation base
Hole 1802, adaptive adjustment part 1751 is arranged in the screwed hole 1802.
In embodiments of the present invention, the connection end of described adaptive adjustment part 1751 and back shroud is the end of thread, and anti-
The connection end for answering storehouse component is the polished rod 1805 for being provided with spring 1806, and nut 1804 is provided between the ends and is positioned,
After installation is complete, nut 1804 is close to installation base.
It is passage 1803 between two described raised lines 1809, enables to Reagent Tube to pass through；Also set on back shroud
It is equipped with the stiffener plate 1812 vertical with raised line 1809；In raised line 1809 and the bit plane of upper surface 1810 of boss；In plate below
Peripheral framework 1807 centre and corner be provided with trip bolt hole 1801, to be connected with described turnable reaction warehouse
It is fixed.
Above-mentioned bonnet board component, is not only able to carry out face sealing to reaction warehouse component, additionally it is possible to install fixed adaptive tune
One integral piece, it is simple in construction.
In embodiments of the present invention, it may be reversed and adaptive adjustment part be provided between reaction warehouse and reaction warehouse base 126
1751, refer to shown in Figure 12, the end spring of adaptive adjustment part 1751 and the end contact of reaction warehouse base；Using
,, can be using movable side when adjusting camera position when camera is with reaction chip component touch when CCD camera is fed
Formula is adjusted, now, and adaptive adjustment part can change the position of reaction chip component according to the change in location of camera, it is ensured that have
Higher accuracy of detection, simultaneously, it is to avoid system is caused to damage.
In embodiments of the present invention, can coaxially it be moved with reaction chip component to ensure may be reversed reaction warehouse component,
Convenient adjustment, is additionally provided with guide rod 1758, refers to shown in Figure 11 c, guide rod is set on reaction warehouse base between the two
Hole 1742, sets guide rod mounting hole 1786, guide rod 1742 sequentially passes through holes on reaction warehouse may be reversed.Described guiding
Bar 1758 includes reaction warehouse locating flange 1757, to be positioned with being oriented to rod aperture 1742；In the other end by nut 1755 with leading
Positioned to the end face of bar mounting hole 1786, pad 1756 is additionally provided between.
In embodiments of the present invention, tested in order to the diverse location to reaction chip, use can change survey
Try the sealing ring 1713 of area；Refer to shown in Figure 14 a-14c, described sealing ring 1713 is hexagon, and it includes outer rim
1716 and the first blend stop 1714 and the second blend stop 1715 of opposite side are arranged on, the first blend stop 1714 and the second blend stop 1715 will examinations
Agent is divided into three parts.In embodiment, the upper end of sidepiece rib and blend stop is respectively the first projection 1718 and second
Projection 1719, two projections cause reagent to be flowed in the range of restriction, and reagent liquid inlet and outlet is arranged between two blend stops
In chamfering.When without using blend stop, reagent is flowed into inside whole sealing ring by the chamfering between two blend stops, now, can
Observe the reaction of whole reaction chip；In state as shown in figures 14a, reaction chip between two blend stops can only be observed
Region；In state as shown in figure 14 c, region that can only be on the right side of observing response chip.
The embodiment of the present invention uses foldable sealing ring, substantially increases the flexibility of reaction test, can be as needed
Reaction chip is sampled.
In embodiments of the present invention, when described reaction warehouse component 12 works, above-mentioned reaction chip component is arranged on can
Overturn in reaction warehouse, install guide rod 1758 and adaptive adjustment part 1751, and packaged back shroud；With turnable basement
After the installation of component 122, locked by electronics lock set and pressing piece and reaction warehouse and door frame may be reversed, and will installed
The reaction warehouse component 12 of completion is arranged on platform component 13.
Above-mentioned detailed description is illustrating for one of them possible embodiments of the present invention, and the embodiment is simultaneously not used to
The scope of the claims of the present invention is limited, all equivalence enforcements or change without departing from carried out by the present invention are intended to be limited solely by the technology of the present invention
In the range of scheme.
Claims (3)
1. the adaptive reaction warehouse of a kind of DNA sequencer, it is characterised in that the adaptive reaction warehouse includes reaction warehouse component, institute
Stating reaction warehouse component includes the door frame being vertically arranged that is arranged on platform component and its is set on the top in the outside of door frame
The turnable reaction warehouse component put；Turnable basement is connected with the lower end that reaction warehouse component may be reversed, and can around described
Base rotation is overturn, described turnable basement is fixed on the middle part of door frame；
The turnable reaction warehouse component includes reaction warehouse may be reversed；Installed in the inner side of the centre of described turnable reaction warehouse
There is reaction chip component, accordingly, the first cavity for accommodating reaction chip component is provided with the centre that reaction warehouse may be reversed；
Described reaction chip component include reaction chip mounting seat, reaction warehouse base assembly and by reaction warehouse base assembly and
The locking of reaction chip mounting seat, the shading positioning component opened, reaction chip mounting seat can be relative with reaction warehouse base assembly
Rotation；
Reaction chip is installed in the center section of reaction chip mounting seat, cover plate is provided with reaction chip；In reaction core
The both sides of piece are provided with chute pressing plate, reaction chip to be fixed；The center section of reaction warehouse base is provided with temperature
Spend sensor mounting groove；
The surrounding of temperature sensor mounting groove is provided with four guide rod mounting holes, its upper and lower sides is provided with adaptive spring
Mounting hole；It is also equipped with being oriented to rod aperture on the cover board；It may be reversed and adaptive adjustment is provided between reaction warehouse and reaction warehouse base
Part, adaptive adjustment part passes through adaptive spring mounting hole, and the end contact of end spring and reaction warehouse base；
Set on reaction warehouse base and be oriented to rod aperture, guide rod mounting hole is set on reaction warehouse may be reversed, guide rod is worn successively
Cross holes；
Reaction chip component, which is arranged on, may be reversed in reaction warehouse, install guide rod and adaptive adjustment part, and it is packaged after
Cover plate；Wherein, the back shroud is located at the center section that reaction warehouse may be reversed, and is provided with certainly in the center section of back shroud
Adapt to adjustment part.
2. the adaptive reaction warehouse of DNA sequencer according to claim 1, it is characterised in that in described back shroud
Two rows are provided with the middle of inner side raised line are installed, the centre for installing raised line is provided with the first installation base and the second installation base,
Screwed hole is offered on installation base, adaptive adjustment part is arranged in the screwed hole.
3. the adaptive reaction warehouse of DNA sequencer according to claim 2, it is characterised in that described adaptive adjustment
The connection end of part and back shroud is the end of thread, and the connection end with reaction warehouse component is the polished rod for being provided with spring, between the ends
It is provided with nut to be positioned, after installation is complete, nut is close to installation base；
It is passage between two described row's raised lines, enables to Reagent Tube to pass through；It is additionally provided with and is hung down with raised line on back shroud
Straight stiffener plate；The upper surface of raised line and boss is plane.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510716595.5A CN105273980B (en) | 2015-10-29 | 2015-10-29 | The DNA sequencer that a kind of reaction warehouse is adaptively adjusted |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510716595.5A CN105273980B (en) | 2015-10-29 | 2015-10-29 | The DNA sequencer that a kind of reaction warehouse is adaptively adjusted |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105273980A CN105273980A (en) | 2016-01-27 |
| CN105273980B true CN105273980B (en) | 2017-09-08 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201510716595.5A Expired - Fee Related CN105273980B (en) | 2015-10-29 | 2015-10-29 | The DNA sequencer that a kind of reaction warehouse is adaptively adjusted |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102703311A (en) * | 2012-05-24 | 2012-10-03 | 中国科学院北京基因组研究所 | Reaction bin for adaptive regulation of DNA (Deoxyribose Nucleic Acid) sequencer |
| CN102703312A (en) * | 2012-05-24 | 2012-10-03 | 中国科学院北京基因组研究所 | DNA (Deoxyribose Nucleic Acid) sequencer |
| CN102703301A (en) * | 2012-05-24 | 2012-10-03 | 中国科学院北京基因组研究所 | Darkroom used between installing seat of sequencing chip and CCD (Charge Coupled Device) camera |
| CN102703302A (en) * | 2012-05-24 | 2012-10-03 | 中国科学院北京基因组研究所 | Installing seat for automatically clamping sequencing chip |
| CN102766574A (en) * | 2012-05-24 | 2012-11-07 | 中国科学院北京基因组研究所 | Reaction chamber for DNA sequenator |
-
2015
- 2015-10-29 CN CN201510716595.5A patent/CN105273980B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102703311A (en) * | 2012-05-24 | 2012-10-03 | 中国科学院北京基因组研究所 | Reaction bin for adaptive regulation of DNA (Deoxyribose Nucleic Acid) sequencer |
| CN102703312A (en) * | 2012-05-24 | 2012-10-03 | 中国科学院北京基因组研究所 | DNA (Deoxyribose Nucleic Acid) sequencer |
| CN102703301A (en) * | 2012-05-24 | 2012-10-03 | 中国科学院北京基因组研究所 | Darkroom used between installing seat of sequencing chip and CCD (Charge Coupled Device) camera |
| CN102703302A (en) * | 2012-05-24 | 2012-10-03 | 中国科学院北京基因组研究所 | Installing seat for automatically clamping sequencing chip |
| CN102766574A (en) * | 2012-05-24 | 2012-11-07 | 中国科学院北京基因组研究所 | Reaction chamber for DNA sequenator |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105273980A (en) | 2016-01-27 |
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Granted publication date: 20170908 Termination date: 20191029 |