CN105267092A - Plant extract and preparation method and application thereof - Google Patents
Plant extract and preparation method and application thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
- A61Q17/005—Antimicrobial preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/52—Stabilizers
- A61K2800/522—Antioxidants; Radical scavengers
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/52—Stabilizers
- A61K2800/524—Preservatives
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/74—Biological properties of particular ingredients
- A61K2800/78—Enzyme modulators, e.g. Enzyme agonists
- A61K2800/782—Enzyme inhibitors; Enzyme antagonists
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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Abstract
The invention discloses plant extract and a preparation method and application thereof. The plant extract is prepared by red flower, saururus chinensis and green papaya. The plant extract has effects of relaxing muscles and stimulating blood circulation, activating meridians to stop pain, diminishing inflammation and sterilizing, removing old cutin, softening the skin, whitening and moisturizing and resisting aging and is capable of effectively removing calluses. In addition, the plant extract is safe to use and non-irritant to the skin.
Description
Technical field:
The present invention relates to cosmetic field, particularly Plant Extracts and its preparation method and application.
Background technology:
Along with the raising of people's living standard, the details place maintenance in the past ignored by people comes into one's own all the more, the maintenance nursing of such as foot.Industry data statistics in 2012 China pedicure shop and pedicure shop just have more than 50,000, and practitioner more than 150 ten thousand, and the market demand is increasing.
As the saying goes " bamboo is withered from leaf, and people is old from foot ", foot is the second heart of human body, all carries the weight of whole body every day, and the aging the easiest of human body embodies from foot.Foot is centrifugal dirty position farthest, and when it is cold, foot vasoconstriction or not smooth because extruding the QI and blood caused, easily brings out various diseases, need to use some products to improve local blood circulation, promote metabolism, play the effect of health care.The sun-proof of foot also often ignored by people, wears sandals and does not carry out again sun-proof meeting on instep, form uneven solarization trace, affect attractive in appearance.Many people especially female friend owing to wearing high-heel shoes, sandals cause the problems such as foot blood is smooth, swelling pain, callus, colour of skin inequality, foot odor, and it is single, larger to the stimulation of skin in the effect realizing the foot cosmetics found in process of the present invention on market, effectively cannot solve the skin problem of foot, and cannot alleviate foot be squeezed the pain that causes and QI and blood not smooth.Most of foot exfoliation product is on the market all use salicylic acid as effective ingredient with removing foot odor product, and salicylic acid improper use excessively can remove horny layer, the phylactic power defensive power of skin can be deteriorated, erythema, pruritus, twinge or allergic phenomena may be there is, also there is the symptom that a few peoples can occur salicylic acid allergy, be not suitable for the salicylic acid using any concentration.The foot frost that protects on the market only has effect of moisturizing and horny layer softening mostly, function singleness and fail effectively to solve callus problem.。
Prevailing along with " back to nature " agitation, the composition of extracted form natural plant is more and more subject to the favor of people, its use safety, effect gentle to skin obviously, there is no dependency.China is vast in territory, natural environment is complicated, plant resources is numerous, utilize long history to plant, but some conventional plant not yet fully develops due value.
Summary of the invention:
In order to overcome above-mentioned the deficiencies in the prior art, the present invention is foundation with theory of Chinese medical science, utilizes modern means of science and technology, provides the preparation method of a Plant Extracts and described plant extract and the application at cosmetic field thereof.
A Plant Extracts of the present invention is prepare with the raw material of following weight fraction: Flos Carthami 30%-40%, Rhizoma Saururi (Herba Saururi) 30%-40%, nonage Fructus Chaenomelis 30%-40%.
Preferably, a described Plant Extracts is prepare with the raw material of following weight fraction: Flos Carthami 33%, Rhizoma Saururi (Herba Saururi) 34%, nonage Fructus Chaenomelis 33%.
The preparation method of plant extract of the present invention, comprises the following steps:
(1) Flos Carthami, Rhizoma Saururi (Herba Saururi) and nonage Fructus Chaenomelis are pulverized respectively, mixing, cross 80 mesh sieves, obtain plant extract, be placed in ultrosonic extractor;
(2) ethanol of 60%-80% will be added in the plant mixture in step (1), wherein the solid-liquid ratio g/ml of plant mixture and ethanol is 1:16-1:20, frequency is adopted to be the ultrasonic Treatment 40-60 minute of 20kHz, in ultrasonic Treatment, ethanol temperature is 40-60 DEG C, filter, collect filtrate A and filtering residue;
(3) filtering residue of step (2) gained is repeated to extract once by ethyl acetate again, wherein filtering residue and ethyl acetate solid-liquid ratio g/ml be 1:8-1:10, frequency is adopted to be the ultrasonic Treatment 30-50 minute of 20kHz, in ultrasonic Treatment, ethyl acetate temperature is 40-50 DEG C, filter, collect liquor B, filtrate A and liquor B are merged to obtain liquor C;
(4) liquor C is placed in Rotary Evaporators, takes out negative pressure to-0.08--0.1MPa, under the condition of 40-45 DEG C, carry out distilling under reduced pressure, be distilled to that volume is original volume 20%, obtain plant extract, bottle for subsequent use.
Preferably, the preparation method of described plant extract, comprises the following steps:
(1) Flos Carthami, Rhizoma Saururi (Herba Saururi) and nonage Fructus Chaenomelis are pulverized respectively, mixing, cross 80 mesh sieves, obtain plant extract, be placed in ultrosonic extractor;
(2) ethanol of 70% will be added in the plant mixture in step (1), wherein the solid-liquid ratio g/ml of plant mixture and ethanol is 1:18, frequency is adopted to be the ultrasonic Treatment 50 minutes of 20kHz, in ultrasonic Treatment, ethanol temperature is 50 DEG C, filter, collect filtrate A and filtering residue;
(3) filtering residue of step (2) gained is repeated to extract once by ethyl acetate again, wherein filtering residue and ethyl acetate solid-liquid ratio g/ml be 1:9, frequency is adopted to be the ultrasonic Treatment 40 minutes of 20kHz, in ultrasonic Treatment, ethyl acetate temperature is 45 DEG C, filter, collect liquor B, filtrate A and liquor B are merged to obtain liquor C;
(4) liquor C is placed in Rotary Evaporators, takes out negative pressure to-0.08--0.1MPa, under the condition of 40-45 DEG C, carry out distilling under reduced pressure, be distilled to that volume is original volume 20%, obtain plant extract, bottle for subsequent use.
Ultrasound wave refers to that frequency is greater than the sound wave of 20kHz, feature be that frequency is high, wavelength is short and penetration power strong etc.The principle of ultrasonic extraction is that ultrasound wave produces cavitation in a liquid, and this effect can destroy the cell wall structure of plant, adds release and the stripping capacity of effective ingredient; The mechanical vibration of ultrasound wave under certain frequency, improve the diffusivity of effective ingredient simultaneously.Hyperacoustic advantage is that equipment is simple, improves raw material availability and productive rate, shortens extraction time, without the need to heating, protect thermally labile component simultaneously.
Flos Carthami is the good medicine of promoting blood circulation to remove obstruction in the collateral, stasis-dispelling and pain-killing, also once uses Flos Carthami decoct to treat callus in traditional Chinese medical science field.Research finds, Flos Carthami extract can make the vasodilation of laboratory animal, the open number increase of capillary network and Blood cell accumulation degree can also be made to alleviate, effective blood circulation promoting.And there is certain anti-inflammatory and antalgic activity, loosen muscular swelling.Adopt DPPH method to carry out research to the antioxygenic property of Flos Carthami to find, Flos Carthami has antioxidant activity, effectively can reduce cell oxidative damage, delay skin aging, skin texture is attenuated greasy.
DPPH is 1,1-diphenyl-2-trinitrophenyl-hydrazine
The abbreviation of (1,1-Diphenyl-2-picrylhydrazylradical2,2-Diphenyl-1-(2,4,6-trinitrophenyl) hydrazyl).DPPH is a kind of stable free radical in organic solvent, and its alcoholic solution is purple, and needs low-temperature dark to store, and has single electronics, therefore can accept an electronics or hydrion, under wavelength is 517nm, have absorption maximum.When having free radical scavenger to exist, the single electron of DPPH is captured and makes its lighter, and the light absorption value at maximum light absorption wavelength place declines, and decline degree is linear, the reduction of level of absorbance shows the increase of non-oxidizability, thus with the oxidation resistance of evaluation test sample.DPPH method be exactly when utilizing DPPH to have free radical scavenger to exist in organic solvent its single electron pairing and make the character that its light absorption fades away, spectrophotometer is utilized to carry out quantitative analysis fast, and then the method for the oxidation resistance of mensuration free radical scavenger.Be widely used in the oxidation resistance of quantitative assay Biosample and food.
Rhizoma Saururi (Herba Saururi) is commonly used for the effect of removing toxic substances, diuresis, detumescence in the traditional Chinese medical science.Adopt B16 melanoma cell as subject cell, research finds that Rhizoma Saururi extract can suppress the propagation of B16 melanoma cell and the activity of intracellular tyrosine enzyme, and obviously reduce the quantity of intracellular melanin, play good whitening effect, by the contrast test of same concentrations, the whitening effect of Rhizoma Saururi (Herba Saururi) be better than arbutin and heliosensitivity far below arbutin, also can use safely in illumination.Adopt paper disk method, the bacteriostasis property of research Rhizoma Saururi extract, result illustrates that Rhizoma Saururi extract has good inhibitory action to mainly causing the staphylococcus aureus of foot odor.
Nonage Fructus Chaenomelis is a kind of common vegetables and fruits, also uses as Chinese medicine simultaneously, has suppressing the hyperactive liver relaxing muscles and tendons, the effect of stomach function regulating removing dampness.Fructus Chaenomelis ferment in nonage Fructus Chaenomelis, vitamin A and fruit acid energy gentleness remove old cutin effectively, promote the homergy of keratodermatitis, improve skin to the absorption efficiency of active component, keep skin delicate.Flavone in nonage Fructus Chaenomelis effectively can remove DPPH, has good antioxidation, and is rich in polysaccharide in nonage Fructus Chaenomelis, as natural wetting agent, skin beauty water can be kept to moisten fine and smooth.Be rich in vitamin C, melanin can be made thin out, make skin recover pale.
Plant extract of the present invention proves to have strong anti-oxidation through overtesting, prevent the tissue of radical pair body and cell from causing damage, thus delay skin aging; There is obvious inhibitory action to tyrosinase activity, reduce melanic formation, there is obvious white-skinned face function; There is inhibitory action to escherichia coli and staphylococcus aureus, multiplying of pernicious bacteria on skin can be reduced, alleviate because bacterial reproduction decomposes the stink that keratin and lactic acid causes; Can promote skin metabolism, the sebum and aging cutin piled up in pore are dissolved in help, make the soft exquisiteness of skin; Inflammatory reaction capable of inhibiting cell, minimizing expression of inflammatory cytokines, the symptom reduced inflammation; The vasodilation of laboratory animal can be made, the open number increase of capillary network and Blood cell accumulation degree can also be made to alleviate, improve hemorheological property, effective blood circulation promoting.Described plant extract makes its sensitization reduce via the synergism of three kind of plant compositions.
The plant extract containing Flos Carthami, Rhizoma Saururi (Herba Saururi), nonage Fructus Chaenomelis using method of the present invention to extract can be used as effective ingredient and directly joins in cosmetics.
The invention provides described plant extract and preparing the application in foodcare cosmetics, wherein in some embodiments, described cosmetics for protecting sufficient frost, protect sufficient emulsion, foot mask essence.
The invention provides one and protect sufficient frost, described in protect the component that sufficient frost comprises following percentage by weight:
Sorbitan stearate: 0.6-1.0wt%,
PEG-60 Span60: 1-1.4wt%,
Glyceryl stearate/PEG-100 stearate: 0.5-1.5wt%,
Cetearyl alcohol: 2-6wt%,
Stearic acid: 1-3wt%,
Palmic acid: 1-3wt%,
White mineral oil: 3-7wt%,
Vaseline: 1-3wt%,
Polydimethylsiloxane: 0.3-0.7wt%,
Caprylic/capric triglyceride: 3-7wt%,
Methyl hydroxybenzoate: 0.1-0.14wt%,
Propyl hydroxybenzoate: 0.06-0.1wt%,
Glycerol: 3-7wt%,
Propylene glycol: 2-4wt%,
EDETATE SODIUM: 0.02-0.04wt%,
Xanthan gum: 0.06-0.1wt%,
Silicon stone: 0.3wt%,
Potassium hydroxide: 0.06wt%,
Sodium acrylate/sodium acryloyldimethyl taurate copolymers/Polyoxyethylene Sorbitan Monooleate/2-Methylpentadecane: 0.3-0.7wt%,
Methylisothiazolinone: 0.06-0.1wt%,
Plant extract: 2-8wt%,
Deionized water: surplus.
Described plant extract be obtain by the preparation method of plant extract of the present invention Flos Carthami, Rhizoma Saururi (Herba Saururi) and Fructus Chaenomelis mixed extract.
The invention provides one and protect sufficient emulsion, described in protect the component that sufficient emulsion comprises following percentage by weight:
Sorbitan stearate: 1.3-1.7wt%
PEG-60 Span60: 1.6-2.0wt%,
Glyceryl stearate/PEG-100 stearate: 1-3wt%,
Cetearyl alcohol: 0.3-0.7wt%,
Isopropyl myristate: 4-5wt%,
White mineral oil: 8-10wt%,
Polydimethylsiloxane: 1-3wt%,
Butylated hydroxytoluene: 0.03-0.07wt%,
Methyl hydroxybenzoate: 0.1-0.14wt%,
Propyl hydroxybenzoate: 0.06-0.1wt%,
Glycerol: 3-5wt%,
Sorbitol: 6-10wt%,
Xanthan gum: 0.1-0.14wt%,
Acrylate/C10-30 alkyl acrylate cross-linked polymer: 0.1-0.3wt%,
Silicon stone: 0.3-0.7wt%,
Citric acid: 0.03-0.05wt%,
Polyacrylamide/C13-14 isoparaffin/laureth-7:0.3-0.5wt%,
Bisabolol: 0.2-0.4wt%,
Potassium hydroxide: 0.06-0.08wt%,
Vitamin A palmitate: 0.3-0.5wt%,
Carbamide: 3-5wt%,
Methylisothiazolinone: 0.06-0.1wt%,
Plant extract: 2-8wt%,
Deionized water: surplus.
Described plant extract be obtain by the preparation method of plant extract of the present invention Flos Carthami, Rhizoma Saururi (Herba Saururi) and Fructus Chaenomelis mixed extract.
The invention provides a kind of foot mask essence, described foot mask essence comprises the component of following percentage by weight:
Polyvinyl alcohol: 10-20wt%,
Glycyrrhizic acid dipotassium: 0.1-0.3wt%,
Cremophor RH40: 0.6-0.8wt%,
Diazolidinyl urea/iodine propilolic alcohol butyl mephenesin Carbamate: 0.2-0.4wt%,
Ethanol: 4-6wt%,
Plant extract: 2-8wt%,
Deionized water: surplus.
Described plant extract be obtain by the preparation method of plant extract of the present invention Flos Carthami, Rhizoma Saururi (Herba Saururi) and Fructus Chaenomelis mixed extract.
When preparing cosmetics, described plant extract accounts for the 2-8% of total formulation weight.
To sum up, the present invention has the following advantages for the plant extract of foodcare:
Preparation technology is simple, and extraction efficiency is high, and preparation time is short, the recyclable recycling of solvent, and Extracting temperature mental retardation available protecting bioactive ingredients is from high temperature.
Have relax through invigorating blood circulation, removing obstruction in the collateral to relieve pain, anti-inflammation and sterilization, the old cutin of removal, softening skin, whitening skin and preserving moisture, antidotal effect, lower relative to sensitization folk prescription extract, to skin milder.There is obvious non-oxidizability and certain bacteriostasis property, the use amount of antiseptic in cosmetics can be reduced, alleviate the stimulation to skin further.
Multiple-effect is unified, and reduces skin protection step, easy to use.
Detailed description of the invention:
Below with reference to embodiment, the present invention will be further described.
Table 1 embodiment 1-4 and comparative example 1-3 adopts the contrast of proportioning raw materials:
Embodiment 1-4 and comparative example 1-3 is by following technique prepared plant extracts:
1, by dry, the pulverizing respectively of the plant of said ratio consumption, 80 mesh sieves were mixed.
2,1800mL70% ethanol is added.Adopt frequency to be the ultrasonic Treatment 50 minutes of 20kHz, in ultrasonic Treatment, ethanol temperature is 50 DEG C.
3, tentatively filter with filtering accuracy 100 object filter material, re-use 1000 object filter clothes and carry out second time filtration, obtain filtrate A and seal for subsequent use.
4, in filtering residue, add the ethyl acetate of 900mL.Adopt frequency to be the ultrasonic Treatment 35 minutes of 20kHz, in ultrasonic Treatment, ethyl acetate temperature is 45 DEG C.
5, tentatively filter with filtering accuracy 100 object filter material, re-use 1000 object filter clothes and carry out second time filtration, obtain liquor B, filtrate A and liquor B merging obtain liquor C, seal for subsequent use.
6, liquor C is placed in Rotary Evaporators, takes out negative pressure to-0.08--0.1MPa, under the condition of 40-45 DEG C, carry out distilling under reduced pressure, be distilled to that volume is original volume 20%, obtain plant extract, bottle for subsequent use.
Embodiment more than 5 irritation test
(1) test specimen: according to the plant extract obtained by embodiment 1 ~ 4, is divided into tested material a1, a2, a3, a4 respectively after waiting water gaging dilution; Flos Carthami extract obtained by comparative example 1, the Rhizoma Saururi extract obtained by comparative example 2, the Fructus Chaenomelis extract obtained by comparative example 3 respectively with etc. water gaging dilution after be divided into b1, b2, b3;
(2) experimental animal: the adult white new zealand rabbit of health, skin zero damage, regular grade, totally 32, body weight is 2.2-2.5kg, thered is provided (animal productiong credit number: SCXK (Guangdong) 2003-0002) by Guangdong Medical Lab Animal Center, the experimental animal quality certification is numbered: 0003296, laboratory animal occupancy permit number: SYXK (Guangdong) 2003-0011, the temperature of Animal House is 23 DEG C, humidity is 52%RH, drinking water is tap water, carries out random packet before experiment, often organizes 4;
(3) test procedure:
1. cut by hair spinal column both sides, laboratory animal back before test, unhairing scope is respectively 3cmX3cm, application area 2.5cmX2.5cm;
2. O.5g get tested material spreads upon on the skin of side, and opposite side, as blank, smears 1 every day, smears 14 days continuously.From second day, smear front cropping at every turn, remain tested material, observed result after a hour with water or nonirritant solvent cleaning, the standards of grading specified according to " cosmetics health specification " (2007 editions) are marked.
(4) experimental result:
Table 2 tested material a1, a2, a3, a4 many skin irritation reaction results
Table 3 tested material b1, b2, b3, blank be skin irritation reaction result repeatedly
(5) evaluation of result
Every day every, animal average integral=Σ erythema and edema integration/animal subject number/14, judged skin irritation intensity with table 4;
Table 4 skin irritation strength grading
| Integral mean value | Intensity |
| 0～<0.5 | Nonirritant |
| 0.5～<2.0 | Subexcite |
| 2.0～<6.0 | Moderate stimulation |
| 6.0～8.0 | Strong and stimulating |
Can find out tested material a1, the equal nonirritant of a3, a4 of experimental group from table 2-4, and tested material a2 is subexcite; The tested material of matched group is subexcite, prove to use the plant extract obtained prepared by proportioning raw materials of the present invention thus obviously little than the zest of folk prescription plant extract, the plant extract using preparation method of the present invention obtained is to the comparatively safe nonirritant of skin.
Embodiment 6
Raw material (g) is taken according to following proportioning:
Flos Carthami 33, Rhizoma Saururi (Herba Saururi) 34, nonage Fructus Chaenomelis 33
Preparation technology is as follows:
1, by dry, the pulverizing respectively of the plant of said ratio consumption, 80 mesh sieves were mixed.
2,1400mL50% ethanol is added.Adopt frequency to be the ultrasonic Treatment 30 minutes of 20kHz, in ultrasonic Treatment, ethanol temperature is 30 DEG C.
3, tentatively filter with filtering accuracy 100 object filter material, re-use 1000 object filter clothes and carry out second time filtration, obtain filtrate A and seal for subsequent use.
4, in filtering residue, add the ethyl acetate of 700mL.Adopt frequency to be the ultrasonic Treatment 20 minutes of 20kHz, in ultrasonic Treatment, ethyl acetate temperature is 35 DEG C.
5, tentatively filter with filtering accuracy 100 object filter material, re-use 1000 object filter clothes and carry out second time filtration, obtain liquor B, filtrate A and liquor B merging obtain liquor C, seal for subsequent use.
6, liquor C is placed in Rotary Evaporators, takes out negative pressure to-0.08--0.1MPa, under the condition of 40-45 DEG C, carry out distilling under reduced pressure, be distilled to that volume is original volume 20%, obtain plant extract, bottle for subsequent use.
Embodiment 7
Raw material (g) is taken according to following proportioning:
Flos Carthami 33, Rhizoma Saururi (Herba Saururi) 34, nonage Fructus Chaenomelis 33
Preparation technology is as follows:
1, by dry, the pulverizing respectively of the plant of said ratio consumption, 80 mesh sieves were mixed.
2,1600mL60% ethanol is added.Adopt frequency to be the ultrasonic Treatment 40 minutes of 20kHz, in ultrasonic Treatment, ethanol temperature is 40 DEG C.
3, tentatively filter with filtering accuracy 100 object filter material, re-use 1000 object filter clothes and carry out second time filtration, obtain filtrate A and seal for subsequent use.
4, in filtering residue, add the ethyl acetate of 800mL.Adopt frequency to be the ultrasonic Treatment 30 minutes of 20kHz, in ultrasonic Treatment, ethyl acetate temperature is 40 DEG C.
5, tentatively filter with filtering accuracy 100 object filter material, re-use 1000 object filter clothes and carry out second time filtration, obtain liquor B, filtrate A and liquor B merging obtain liquor C, seal for subsequent use.
6, liquor C is placed in Rotary Evaporators, takes out negative pressure to-0.08--0.1MPa, under the condition of 40-45 DEG C, carry out distilling under reduced pressure, be distilled to that volume is original volume 20%, obtain plant extract, bottle for subsequent use.
Embodiment 8
Raw material (g) is taken according to following proportioning:
Flos Carthami 33, Rhizoma Saururi (Herba Saururi) 34, nonage Fructus Chaenomelis 33
Preparation technology is as follows:
1, by dry, the pulverizing respectively of the plant of said ratio consumption, 80 mesh sieves were mixed.
2,2000mL80% ethanol is added.Adopt frequency to be the ultrasonic Treatment 60 minutes of 20kHz, in ultrasonic Treatment, ethanol temperature is 60 DEG C.
3, tentatively filter with filtering accuracy 100 object filter material, re-use 1000 object filter clothes and carry out second time filtration, obtain filtrate A and seal for subsequent use.
4, in filtering residue, add the ethyl acetate of 1000mL.Adopt frequency to be the ultrasonic Treatment 50 minutes of 20kHz, in ultrasonic Treatment, ethyl acetate temperature is 50 DEG C.
5, tentatively filter with filtering accuracy 100 object filter material, re-use 1000 object filter clothes and carry out second time filtration, obtain liquor B, filtrate A and liquor B merging obtain liquor C, seal for subsequent use.
6, liquor C is placed in Rotary Evaporators, takes out negative pressure to-0.08--0.1MPa, under the condition of 40-45 DEG C, carry out distilling under reduced pressure, be distilled to that volume is original volume 20%, obtain plant extract, bottle for subsequent use.
Embodiment 9
Raw material (g) is taken according to following proportioning:
Flos Carthami 33, Rhizoma Saururi (Herba Saururi) 34, nonage Fructus Chaenomelis 33
Preparation technology is as follows:
1, by dry, the pulverizing respectively of the plant of said ratio consumption, 80 mesh sieves were mixed.
2,2200mL90% ethanol is added.Adopt frequency to be the ultrasonic Treatment 70 minutes of 20kHz, in ultrasonic Treatment, ethanol temperature is 70 DEG C.
3, tentatively filter with filtering accuracy 100 object filter material, re-use 1000 object filter clothes and carry out second time filtration, obtain filtrate A and seal for subsequent use.
4, in filtering residue, add the ethyl acetate of 1100mL.Adopt frequency to be the ultrasonic Treatment 60 minutes of 20kHz, in ultrasonic Treatment, ethyl acetate temperature is 55 DEG C.
5, tentatively filter with filtering accuracy 100 object filter material, re-use 1000 object filter clothes and carry out second time filtration, obtain liquor B, filtrate A and liquor B merging obtain liquor C, seal for subsequent use.
6, liquor C is placed in Rotary Evaporators, takes out negative pressure to-0.08--0.1MPa, under the condition of 40-45 DEG C, carry out distilling under reduced pressure, be distilled to that volume is original volume 20%, obtain plant extract, bottle for subsequent use.
Embodiment 10
Flos Carthami extract, Rhizoma Saururi extract, Fructus Chaenomelis extract described in plant extract described in embodiment 1 and embodiment 6-9 and comparative example 1-3 are made and protected sufficient frost.
The present embodiment A1-E1 group is protected in sufficient frost containing plant extraction liquid described in embodiment 1 and embodiment 6-9; Comparative example A 2-C2 group protects foot frost containing Flos Carthami extract, Rhizoma Saururi extract, Fructus Chaenomelis extract described in comparative example 1-3.
A1-E1 group and A2-C2 group protect foot frost component and percentage by weight in table 5:
Table 5
Embodiment 11
Flos Carthami extract, Rhizoma Saururi extract, Fructus Chaenomelis extract described in plant extract described in embodiment 1 and embodiment 6-9 and comparative example 1-3 are made and protected sufficient emulsion.
The present embodiment F1-J1 group is protected in sufficient emulsion containing plant extraction liquid described in embodiment 1 and embodiment 6-9; Comparative example F2-H2 group is protected sufficient emulsion and is contained Flos Carthami extract described in comparative example 1-3, Rhizoma Saururi extract, Fructus Chaenomelis extract.
F1-J1 group and F2-H2 group protect the component of sufficient emulsion and percentage by weight in table 6:
Table 6
Embodiment 12
Flos Carthami extract, Rhizoma Saururi extract, Fructus Chaenomelis extract described in plant extract described in embodiment 1 and embodiment 6-9 and comparative example 1-3 are made foot mask essence.
Containing plant extraction liquid described in embodiment 1 and embodiment 6-9 in the present embodiment K1-O1 group foot mask essence; Comparative example K2-M2 group foot mask essence contains Flos Carthami extract described in comparative example 1-3, Rhizoma Saururi extract, Fructus Chaenomelis extract.
The component of K1-O1 group and K2-M2 group foot mask essence and percentage by weight are in table 7:
Table 7
Embodiment 13
The present embodiment is the efficacy assessments of cosmetics described in embodiment 10-12.
One, experiment purpose
Extracted by Flos Carthami extract described in the material extract described in relative analysis Evaluation operation example 1, embodiment 6-9 and comparative example 1-3, Rhizoma Saururi (Herba Saururi), Fructus Chaenomelis extract and by the aging resistance of its obtained cosmetics, whitening, moisturizing skin, remove the effect of old cutin, antibacterial, invigorating blood circulation analgesia.
Two, experimental technique
100 volunteers collecting pedal skin not good try out above-mentioned each cosmetics after 28 days, observed result.Wherein, women 60 people, male 40 people, max age 45 years old, minimal ages 22 years old, 26 years old mean age.
The aging resistance of described cosmetics, whitening, moisturizing skin, remove old cutin, efficacy validation that is antibacterial, invigorating blood circulation analgesia shows as: xerosis cutis is coarse, wrinkle, dark yellow, the colour of skin is uneven, callus, foot odor, the improvement effect that swells and ache.
Three, evaluation of result
Continuous use is after 28 days, observed result, is invalidly designated as 0 point; Moderate effect is had to remember 1 point; Positive effect is had to remember 2 points.Score full marks are 200, and mark is higher, represent that its effect is more remarkable.
Four, experimental result
Table 8 for use obtained by embodiment A 1-E1 and comparative example A 2-C2 protect sufficient frost after, wrinkle coarse to xerosis cutis, dark yellow, the colour of skin is uneven, callus, foot odor, the observed result of change swollen and ache and to skin irritation reaction observed result:
Table 8
Table 9 for use obtained by embodiment F 1-J1 and comparative example F2-H2 protect sufficient emulsion after, wrinkle coarse to xerosis cutis, dark yellow, the colour of skin is uneven, callus, foot odor, the observed result of change swollen and ache and to skin irritation reaction observed result:
Table 9
Table 10 is coarse to xerosis cutis after using the foot mask essence obtained by embodiment K1-O1 and comparative example K2-M2, wrinkle, dark yellow, the colour of skin is uneven, callus, foot odor, the observed result of change swollen and ache and to skin irritation reaction observed result:
Table 10
From table 8, table 9 and table 10 data can be found out, add separately Flos Carthami extract ratio group, add separately Rhizoma Saururi extract ratio group and add separately Fructus Chaenomelis withdrawal ratio group, no matter coarse to xerosis cutis, wrinkle, dark yellow, the colour of skin is uneven, callus, foot odor, swell and ache and all improve significantly, and the composite use of three kind of plant is coarse to xerosis cutis, wrinkle, dark yellow, the colour of skin is uneven, callus, foot odor, the improvement of swelling and ache all has obvious facilitation, three kind of plant are by after compatibility simultaneously, its extract joins to significantly reduce when using in cosmetics series and is used alone Flos Carthami extract, Rhizoma Saururi extract or nonage Fructus Chaenomelis extract add the zest to skin when using in cosmetics series.The effect being prepared from plant extract according to the processing technology of embodiment 1 is the most remarkable.
The above, it is only preferred embodiment of the present invention, not any pro forma restriction is done to the present invention, therefore all contents not departing from technical solution of the present invention, according to technical spirit of the present invention to any simple modification made for any of the above embodiments, equivalent variations and modification, all still belong in the scope of technical solution of the present invention.
Claims (9)
1. a Plant Extracts, is characterized in that, is to prepare with the raw material of following percentage by weight: Flos Carthami 30%-40%, Rhizoma Saururi (Herba Saururi) 30%-40%, nonage Fructus Chaenomelis 30%-40%.
2. plant extract according to claim 1, is characterized in that, is to prepare with the raw material of following percentage by weight: Flos Carthami 33%, Rhizoma Saururi (Herba Saururi) 34%, nonage Fructus Chaenomelis 33%.
3. a preparation method for plant extract according to claim 1 and 2, is characterized in that, comprises the following steps:
(1) Flos Carthami, Rhizoma Saururi (Herba Saururi) and nonage Fructus Chaenomelis are weighed by proportioning, pulverize, mixing, obtains plant mixture;
(2) ethanol of 60%-80% will be added in the plant mixture in step (1), wherein the solid-liquid ratio g/ml of plant mixture and ethanol is 1:16-1:20, ultrasonic Treatment 40-60 minute, and temperature is 40-60 DEG C, filter, collect filtrate A and filtering residue;
(3) filtering residue of step (2) gained is repeated to extract once by ethyl acetate again, wherein filtering residue and ethyl acetate solid-liquid ratio g/ml be 1:8-1:10, ultrasonic Treatment 30-50 minute, temperature is 40-50 DEG C, filter, collect liquor B, filtrate A and liquor B are merged to obtain liquor C;
(4) liquor C is carried out distilling under reduced pressure, be distilled to that volume is original volume 20%, obtain plant extract.
4. the preparation method of plant extract according to claim 3, is characterized in that, comprises the following steps:
(1) Flos Carthami, Rhizoma Saururi (Herba Saururi) and nonage Fructus Chaenomelis are weighed by proportioning, pulverize, mixing, obtains plant mixture;
(2) will add the ethanol of 70% in the plant mixture in step (1), wherein the solid-liquid ratio g/ml of plant mixture and ethanol is 1:18, ultrasonic Treatment 50 minutes, and temperature is 50 DEG C, filters, and collects filtrate A and filtering residue;
(3) filtering residue of step (2) gained is repeated to extract once by ethyl acetate again, wherein filtering residue and ethyl acetate solid-liquid ratio g/ml be 1:9, ultrasonic Treatment 40 minutes, temperature is 45 DEG C, filter, collect liquor B, filtrate A and liquor B are merged to obtain liquor C;
(4) liquor C is carried out distilling under reduced pressure, be distilled to that volume is original volume 20%, obtain plant extract.
5. the plant extract according to any one of claim 1-4 is preparing the application in foodcare cosmetics.
6. application according to claim 5, is characterized in that: described foodcare cosmetics are for protecting sufficient frost, protecting sufficient emulsion and foot mask essence.
7. protect a sufficient frost, it is characterized in that, described in protect the component that sufficient frost comprises following percentage by weight:
Sorbitan stearate: 0.6-1.0wt%,
PEG-60 Span60: 1-1.4wt%,
Glyceryl stearate/PEG-100 stearate: 0.5-1.5wt%,
Cetearyl alcohol: 2-6wt%,
Stearic acid: 1-3wt%,
Palmic acid: 1-3wt%,
White mineral oil: 3-7wt%,
Vaseline: 1-3wt%,
Polydimethylsiloxane: 0.3-0.7wt%,
Caprylic/capric triglyceride: 3-7wt%,
Methyl hydroxybenzoate: 0.1-0.14wt%,
Propyl hydroxybenzoate: 0.06-0.1wt%,
Glycerol: 3-7wt%,
Propylene glycol: 2-4wt%,
EDETATE SODIUM: 0.02-0.04wt%,
Xanthan gum: 0.06-0.1wt%,
Silicon stone: 0.3wt%,
Potassium hydroxide: 0.06wt%,
Sodium acrylate/sodium acryloyldimethyl taurate copolymers/Polyoxyethylene Sorbitan Monooleate/2-Methylpentadecane: 0.3-0.7wt%,
Methylisothiazolinone: 0.06-0.1wt%,
Plant extract as described in any one of claim 1-4: 2-8wt%,
Deionized water: surplus.
8. protect a sufficient emulsion, it is characterized in that, described in protect the component that sufficient emulsion comprises following percentage by weight:
Sorbitan stearate: 1.3-1.7wt%
PEG-60 Span60: 1.6-2.0wt%,
Glyceryl stearate/PEG-100 stearate: 1-3wt%,
Cetearyl alcohol: 0.3-0.7wt%,
Isopropyl myristate: 4-5wt%,
White mineral oil: 8-10wt%,
Polydimethylsiloxane: 1-3wt%,
Butylated hydroxytoluene: 0.03-0.07wt%,
Methyl hydroxybenzoate: 0.1-0.14wt%,
Propyl hydroxybenzoate: 0.06-0.1wt%,
Glycerol: 3-5wt%,
Sorbitol: 6-10wt%,
Xanthan gum: 0.1-0.14wt%,
Acrylate/C10-30 alkyl acrylate cross-linked polymer: 0.1-0.3wt%,
Silicon stone: 0.3-0.7wt%,
Citric acid: 0.03-0.05wt%,
Polyacrylamide/C13-14 isoparaffin/laureth-7:0.3-0.5wt%,
Bisabolol: 0.2-0.4wt%,
Potassium hydroxide: 0.06-0.08wt%,
Vitamin A palmitate: 0.3-0.5wt%,
Carbamide: 3-5wt%,
Methylisothiazolinone: 0.06-0.1wt%,
Plant extract as described in any one of claim 1-4: 2-8wt%,
Deionized water: surplus.
9. a foot mask essence, is characterized in that, described foot mask essence comprises the component of following percentage by weight:
Polyvinyl alcohol: 10-20wt%,
Glycyrrhizic acid dipotassium: 0.1-0.3wt%,
Cremophor RH40: 0.6-0.8wt%,
Diazolidinyl urea/iodine propilolic alcohol butyl mephenesin Carbamate: 0.2-0.4wt%,
Ethanol: 4-6wt%,
Plant extract as described in any one of claim 1-4: 2-8wt%,
Deionized water: surplus.
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| CN105662966A (en) * | 2016-03-02 | 2016-06-15 | 广州丹奇日用化工厂有限公司 | Anti-aging plant extract composition |
| CN105708757A (en) * | 2016-02-17 | 2016-06-29 | 佛山市芊茹化妆品有限公司 | Antiallergic microemulsion |
| CN106344436A (en) * | 2016-08-31 | 2017-01-25 | 苏州菲博特环保科技有限公司 | Skin-tendering, moisturizing and cutin-eliminating hand membrane and preparation method thereof |
| CN106377473A (en) * | 2016-09-30 | 2017-02-08 | 防城港市港口区高创信息技术有限公司 | Plant composition and preparation method and application thereof |
| CN109091449A (en) * | 2018-10-31 | 2018-12-28 | 广东巴松那生物科技有限公司 | A kind of set face cream and the sleep mask of gel and preparation method thereof |
| CN109512734A (en) * | 2017-11-24 | 2019-03-26 | 北京东方淼森生物科技有限公司 | Have effects that restore exterior-applied plant extract, cosmetics of elasticity of skin and gloss and preparation method thereof |
| CN109512735A (en) * | 2018-01-10 | 2019-03-26 | 北京东方淼森生物科技有限公司 | Have effects that exterior-applied plant extract, cosmetics of whitening skin lightening and preparation method thereof |
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| CN115177551A (en) * | 2022-08-19 | 2022-10-14 | 广州陶妆生物科技有限公司 | Cosmetic additive |
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| CN105708757A (en) * | 2016-02-17 | 2016-06-29 | 佛山市芊茹化妆品有限公司 | Antiallergic microemulsion |
| CN105662966A (en) * | 2016-03-02 | 2016-06-15 | 广州丹奇日用化工厂有限公司 | Anti-aging plant extract composition |
| CN106344436A (en) * | 2016-08-31 | 2017-01-25 | 苏州菲博特环保科技有限公司 | Skin-tendering, moisturizing and cutin-eliminating hand membrane and preparation method thereof |
| CN106377473A (en) * | 2016-09-30 | 2017-02-08 | 防城港市港口区高创信息技术有限公司 | Plant composition and preparation method and application thereof |
| CN109512734A (en) * | 2017-11-24 | 2019-03-26 | 北京东方淼森生物科技有限公司 | Have effects that restore exterior-applied plant extract, cosmetics of elasticity of skin and gloss and preparation method thereof |
| CN109512756A (en) * | 2017-11-24 | 2019-03-26 | 北京东方淼森生物科技有限公司 | The cosmetics addition extracting method and extract of plant material |
| CN109512734B (en) * | 2017-11-24 | 2021-04-16 | 北京东方淼森生物科技有限公司 | External plant extract and cosmetic with skin elasticity and gloss recovery effects and preparation method thereof |
| CN109512756B (en) * | 2017-11-24 | 2021-05-04 | 北京东方淼森生物科技有限公司 | Method for extracting plant material for cosmetic additive and extract |
| CN109512735A (en) * | 2018-01-10 | 2019-03-26 | 北京东方淼森生物科技有限公司 | Have effects that exterior-applied plant extract, cosmetics of whitening skin lightening and preparation method thereof |
| CN109512735B (en) * | 2018-01-10 | 2021-04-16 | 北京东方淼森生物科技有限公司 | External plant extract with whitening and skin brightening effects, cosmetic and preparation method thereof |
| CN109091449A (en) * | 2018-10-31 | 2018-12-28 | 广东巴松那生物科技有限公司 | A kind of set face cream and the sleep mask of gel and preparation method thereof |
| CN115177551A (en) * | 2022-08-19 | 2022-10-14 | 广州陶妆生物科技有限公司 | Cosmetic additive |
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