CN105265998A - Preparation method of antarctic krill low-fluoride enzymatic hydrolysate - Google Patents
Preparation method of antarctic krill low-fluoride enzymatic hydrolysate Download PDFInfo
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- 241000239366 Euphausiacea Species 0.000 title claims abstract description 58
- 238000002360 preparation method Methods 0.000 title claims abstract description 20
- 230000002255 enzymatic effect Effects 0.000 title abstract 7
- 239000000413 hydrolysate Substances 0.000 title abstract 7
- 102000004190 Enzymes Human genes 0.000 claims abstract description 40
- 108090000790 Enzymes Proteins 0.000 claims abstract description 40
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 claims abstract description 31
- 239000011575 calcium Substances 0.000 claims abstract description 31
- 229910052791 calcium Inorganic materials 0.000 claims abstract description 31
- 239000011737 fluorine Substances 0.000 claims abstract description 25
- 229910052731 fluorine Inorganic materials 0.000 claims abstract description 25
- 238000000034 method Methods 0.000 claims abstract description 17
- 239000000796 flavoring agent Substances 0.000 claims abstract description 11
- 235000019634 flavors Nutrition 0.000 claims abstract description 11
- 239000003957 anion exchange resin Substances 0.000 claims abstract description 9
- 238000006115 defluorination reaction Methods 0.000 claims abstract description 6
- BHPQYMZQTOCNFJ-UHFFFAOYSA-N Calcium cation Chemical compound [Ca+2] BHPQYMZQTOCNFJ-UHFFFAOYSA-N 0.000 claims abstract description 3
- WUKWITHWXAAZEY-UHFFFAOYSA-L calcium difluoride Chemical compound [F-].[F-].[Ca+2] WUKWITHWXAAZEY-UHFFFAOYSA-L 0.000 claims abstract description 3
- 229910001634 calcium fluoride Inorganic materials 0.000 claims abstract description 3
- 229910001424 calcium ion Inorganic materials 0.000 claims abstract description 3
- 239000007788 liquid Substances 0.000 claims description 60
- 238000001179 sorption measurement Methods 0.000 claims description 25
- YCKRFDGAMUMZLT-UHFFFAOYSA-N Fluorine atom Chemical compound [F] YCKRFDGAMUMZLT-UHFFFAOYSA-N 0.000 claims description 24
- 235000013305 food Nutrition 0.000 claims description 19
- 239000011347 resin Substances 0.000 claims description 14
- 229920005989 resin Polymers 0.000 claims description 14
- 235000013372 meat Nutrition 0.000 claims description 13
- 102000035195 Peptidases Human genes 0.000 claims description 10
- 108091005804 Peptidases Proteins 0.000 claims description 10
- 241001465754 Metazoa Species 0.000 claims description 9
- VSGNNIFQASZAOI-UHFFFAOYSA-L calcium acetate Chemical compound [Ca+2].CC([O-])=O.CC([O-])=O VSGNNIFQASZAOI-UHFFFAOYSA-L 0.000 claims description 9
- 229960005147 calcium acetate Drugs 0.000 claims description 9
- 235000011092 calcium acetate Nutrition 0.000 claims description 9
- 239000001639 calcium acetate Substances 0.000 claims description 9
- NIXOWILDQLNWCW-UHFFFAOYSA-N Acrylic acid Chemical class OC(=O)C=C NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 claims description 7
- 238000004140 cleaning Methods 0.000 claims description 7
- 239000008367 deionised water Substances 0.000 claims description 7
- 229910021641 deionized water Inorganic materials 0.000 claims description 7
- 230000007071 enzymatic hydrolysis Effects 0.000 claims description 7
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 claims description 7
- 238000003756 stirring Methods 0.000 claims description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 7
- 238000006243 chemical reaction Methods 0.000 claims description 6
- 238000002203 pretreatment Methods 0.000 claims description 4
- 241000238557 Decapoda Species 0.000 claims description 2
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- 239000000126 substance Substances 0.000 claims description 2
- 102000004169 proteins and genes Human genes 0.000 abstract description 6
- 108090000623 proteins and genes Proteins 0.000 abstract description 6
- 238000011084 recovery Methods 0.000 abstract description 3
- 235000019658 bitter taste Nutrition 0.000 abstract description 2
- 235000018102 proteins Nutrition 0.000 abstract description 2
- 108010009736 Protein Hydrolysates Proteins 0.000 abstract 1
- 235000021120 animal protein Nutrition 0.000 abstract 1
- 238000005119 centrifugation Methods 0.000 abstract 1
- -1 fluorine ions Chemical class 0.000 abstract 1
- 235000015097 nutrients Nutrition 0.000 abstract 1
- 239000000376 reactant Substances 0.000 abstract 1
- 239000007787 solid Substances 0.000 abstract 1
- 239000003795 chemical substances by application Substances 0.000 description 15
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 12
- 239000003463 adsorbent Substances 0.000 description 10
- 238000010521 absorption reaction Methods 0.000 description 6
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 6
- 229910052757 nitrogen Inorganic materials 0.000 description 6
- 230000035484 reaction time Effects 0.000 description 5
- 238000001914 filtration Methods 0.000 description 4
- 239000012528 membrane Substances 0.000 description 4
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- 230000002940 repellent Effects 0.000 description 3
- 239000005871 repellent Substances 0.000 description 3
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- 231100000252 nontoxic Toxicity 0.000 description 2
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- 230000035764 nutrition Effects 0.000 description 2
- 241000251468 Actinopterygii Species 0.000 description 1
- 206010001497 Agitation Diseases 0.000 description 1
- 241000238424 Crustacea Species 0.000 description 1
- 241000239368 Euphausia Species 0.000 description 1
- 241000239370 Euphausia superba Species 0.000 description 1
- 241000239369 Euphausiidae Species 0.000 description 1
- 206010016818 Fluorosis Diseases 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
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- 230000009286 beneficial effect Effects 0.000 description 1
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- 230000015556 catabolic process Effects 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000003651 drinking water Substances 0.000 description 1
- 235000020188 drinking water Nutrition 0.000 description 1
- 230000009977 dual effect Effects 0.000 description 1
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- 230000007613 environmental effect Effects 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- 235000019197 fats Nutrition 0.000 description 1
- 239000012065 filter cake Substances 0.000 description 1
- 238000005188 flotation Methods 0.000 description 1
- 239000013505 freshwater Substances 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Seasonings (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The present invention belongs to the technical field of enzymolysis, and specifically discloses a preparation method of an antarctic krill low-fluoride enzymatic hydrolysate. According to the preparation method, firstly, an antarctic krill enzymatic hydrolysate is prepared by using a complex enzyme composed of an animal protein hydrolase and a flavor enzyme so as to ensure the protein yield while reducing the bitterness of the enzymatic hydrolysate; then, organic calcium is used to replace inorganic calcium as a reactant to precipitate fluorine ions in the hydrolysate; and finally, anion exchange resin is used to absorb calcium fluoride deposit and calcium ions, and centrifugation is carried out to obtain the enzymatic hydrolysate with a high defluorination rate. The antarctic krill low-fluoride enzymatic hydrolysate has a solid recovery rate of 87.3-89.7% and a defluorination rate of 78.1-85.3%. The preparation method provided by the invention is advantaged by simple process, convenient operation, high efficiency and little nutrient loss, is conducive to the further development and utilization of antarctic krill enzymatic hydrolysate, and lays a foundation for comprehensive and industrialized development of antarctic krill.
Description
Technical field
The present invention relates to zymolysis technique field, particularly, relate to the preparation method of the low fluorine enzymolysis liquid of a kind of krill.
Background technology
Krill (
euphausiasuperba) be a kind of small ocean flotation crustaceans animal, be under the jurisdiction of Euphausiacea (
euphausiacea), krill section (
euphausiidae), krill belongs to (
euphausia).Krill afterbody length of growing up is generally 4 ~ 6cm, lives in the mode of trooping.Krill take phytoplankton as Major Foods, plays an important role in Southern Oceans food chain, is the key species of Antarctic ecosystems.Krill resource reserves are huge, estimate between 6.5 hundred million t to 1,000,000,000 t, are can for the one that enrich the most of reserves in the marine animal resource of human use, are also the important protein pools of the mankind.Krill is nutritious, and protein, fat, mineral element equal size are high.Along with the sharply expansion of population in the world, becoming increasingly conspicuous of the problem be short of food, be badly in need of the bioprotein resource that exploitation is new, the huge protein pool that krill enriches as comprehensive nutrition, has wide exploitation prospect.
Fluorine is the common elements in Environmental Chemistry and life science, also be one of trace element of needed by human, dosage is depended in the impact of fluorine on human health, and too much fluorine absorption can bring adverse influence to human body, therefore analyzes to the detection of Fluorine in Foods content the concern being day by day subject to people.Fluorine as one of minority micro-biological element with dual threshold character, in suitable scope, to the construction of biological hard tissue and promote that the aspects such as living organism growth, growth play the irreplaceable effect of other element.Take in appropriate fluorine, for the metabolism etc. of the metabolism of the normal calcium of body, phosphorus, anti-caries, nerve excitability conduction, lipid-metabolism and enzyme system, all there is facilitation; When intake is too high, the normal Ca,P metabolism of body can be destroyed, and the activity of some enzyme can be suppressed, cause a series of disease thus, comprise: den tal fluorosis, fluorosis of bone, kidney, heart, nervous system damage, immunologic dysfunction, endocrine disturbance, degradation under children's intelligence.
According to GB2762-2005 pollutants in food limit standard, fluorine in meat limit standard is 2.0mg/kg, and in fish (fresh water), limit standard is 2.0mg/kg.Oil repellent in krill enzymolysis liquid is up to 40 ~ 70mg/kg, exceed the Oil repellent limitation requirement of country to drinking water and many food far away, in order to better develop krill, we are necessary to carry out defluorinate process to enzymolysis liquid, reduce its Oil repellent.Defluorination method conventional in prior art has nano filtering process, electroosmose process, chemical-biological method.Adopt nano filtering process and electroosmose process to carry out defluorinate to enzymolysis liquid, most salt in enzymolysis liquid can be removed while defluorinate, but in nano filtering process and electroosmose process defluorinate process total nitrogen and ammonia nitrogen loss serious, resultant effect is not satisfactory.Existing chemical-biological fado adopts inorganic calcium to carry out defluorinate, but inorganic calcium defluorinate need to investigate in the security in food applications field.Therefore, lack in prior art and efficiently defluorinate can not lose again the defluorination method of nutrition.
Summary of the invention
The present invention, in order to overcome the above-mentioned deficiency of prior art, provides the preparation method of the low fluorine enzymolysis liquid of a kind of krill.
To achieve these goals, the present invention is achieved by the following technical programs:
A preparation method for the low fluorine enzymolysis liquid of krill, comprises the steps:
S1. utilize the complex enzyme of animal proteolytic enzyme and food flavor enzyme composition to prepare krill enzymolysis liquid, in complex enzyme, the mass ratio of proteolytic enzyme and food flavor enzyme is 1:0.5 ~ 0.8;
S2. utilize organic calcium to carry out chemical method defluorinate process to enzymolysis liquid, described organic calcium is made up of calcium acetate and calcium gluconae, and the mass ratio of calcium acetate and calcium gluconae is 1:0.3 ~ 0.6;
S3. utilize anion exchange resin to adsorb calcium fluoride precipitate and calcium ion, obtain the low fluorine enzymolysis liquid of krill.
The present invention uses the complex enzyme be made up of animal proteolytic enzyme and food flavor enzyme to prepare krill enzymolysis liquid, also reduces the bitter taste of enzymolysis liquid while ensureing albumen yield; The calcium source that the present invention uses is organic calcium, and safety non-toxic, is suitable as food additives and joins in food, and organic calcium defluorinate efficiency compared with inorganic calcium is high, and defluorinate rate is up to 85 ~ 94%.
Preferably, complex enzyme described in S1 according to 0.1 ~ 0.3% mass concentration add.
Preferably, organic calcium described in S2 adds in enzymolysis liquid according to the addition of 0.005 ~ 0.03g/mL.
Preferably, described anion exchange resin is made up of acrylic acid series gel type strong base negative nonionic adsorption resin and macroporous strong-base Anion-adsorption resin, and the mass ratio of the two is 1:0.2 ~ 0.8.
More preferably, described step S1 is specially: after krill cleaning, deionized water is added in 1:0.5 ~ 1.5 ratio in shrimp, homogeneous, obtain meat gruel, addition by 0.1 ~ 0.3% adds complex enzyme in slurries, in 45 ~ 55 DEG C of reaction 3 ~ 4h, the enzyme that goes out, centrifugally obtains krill enzymolysis liquid after enzymolysis.Most preferably, described centrifugal condition, under centrifugal force is 2000 ~ 15000g condition, is crossed the centrifugal 10 ~ 20min of 60 ~ 200 order filter cloth and is obtained krill enzymolysis liquid.
More preferably, before adding organic calcium in S2, controlled enzymatic hydrolysis liquid temp is 25 ~ 35 DEG C, pH 8.5 ~ 10.0, defluorination reaction 0.5 ~ 2h after adding organic calcium.
More preferably, step S3 also comprises pre-treatment step, and being specially control pH is 8.0 ~ 9.5, and temperature is 25 ~ 35 DEG C; Add anion exchange resin after pre-treatment, stirring reaction 0.5 ~ 1h, reaction terminates rear centrifugal, obtains low fluorine krill enzymolysis liquid.Most preferably, described centrifugal condition, under centrifugal force is 4000 ~ 12000g condition, is crossed the centrifugal 10 ~ 20min of 80 ~ 200 order filter cloth and is obtained low fluorine krill enzymolysis liquid.
Compared with prior art, the present invention has following beneficial effect:
In the present invention, calcium source used is organic calcium, and safety non-toxic is suitable as food additives and joins in food, and defluorinate rate is up to 85 ~ 94%, and defluorinate efficiency is high; Adopt centrifugal filtration in the present invention, form filter cake in filter process, under the prerequisite ensureing protein recovery, remove most calcirm-fluoride, simple to operate, protein recovery is high, is conducive to promoting the use of in extensive actual production; The present invention utilizes anion exchange resin adsorption treatment, solves the problem that enzymolysis liquid organic calcium and calcirm-fluoride are residual, realizes defluorinate noresidue.Anion exchange resin can use by repeated regeneration, and cost is low, can be generalized in actual production and uses.
Detailed description of the invention
To make the present invention below in conjunction with specific embodiment and elaborating further, described embodiment, only for explaining the present invention, is not intended to limit scope of the present invention.The test method used in following embodiment if no special instructions, is conventional method; The material used, reagent etc. if no special instructions, are the reagent that can obtain from commercial channels and material.
embodiment 1
S1. enzymolysis liquid preparation: animal proteolytic enzyme and food flavor enzyme are carried out being mixed to form complex enzyme according to the mass ratio of 1:0.8; After krill cleaning, add deionized water in the ratio of 1:1, homogeneous obtains meat gruel, and in meat gruel, add the complex enzyme of 0.2%, slowly stir enzymolysis, enzymatic hydrolysis condition is 50 DEG C, enzymolysis 3.5h.Go out after enzymolysis completes enzyme 10min at 100 DEG C, and cool to room temperature crosses 200 order filter clothes under the condition of 3000g, and centrifugal 10Min, obtains krill enzymolysis liquid.
S2. defluorinate: add defluorinating agent in enzymolysis liquid, in defluorinating agent, the mass ratio of calcium acetate and calcium gluconae is 1:0.6, defluorinate condition is: pH9.5, temperature 35 DEG C, reaction time 1.5h, defluorinating agent addition 0.02g/mL, defluorinate terminates rear interpolation large biological molecule adsorbent, acrylic acid series gel type strong base negative nonionic adsorption resin and macroporous strong-base Anion-adsorption mixed with resin ratio are 1:0.5, adsorption conditions: pH9.0, temperature 30 DEG C, adsorption time 1h, adsorbent addition 0.05g/mL, after absorption, enzymolysis liquid is crossed under the condition of 5000g that 200 order filter clothes are centrifugal obtains low fluorine krill enzymolysis liquid.The defluorinate rate of the present embodiment to krill enzymolysis liquid is 81.3%, and total nitrogen and ammonia nitrogen loss are respectively 3.48%, 4.52%.
embodiment 2
S1. enzymolysis liquid preparation: animal proteolytic enzyme and food flavor enzyme are carried out being mixed to form complex enzyme according to the mass ratio of 1:0.6; After krill cleaning, add deionized water in the ratio of 1:0.5, homogeneous obtains meat gruel, and in meat gruel, add the complex enzyme of 0.15%, slowly stir enzymolysis, enzymatic hydrolysis condition is 45 DEG C, enzymolysis 4h.Go out after enzymolysis completes enzyme 10min at 100 DEG C, and cool to room temperature crosses 100 order filter clothes under the condition of 5000g, and centrifugal 15Min, obtains krill enzymolysis liquid.
S2. defluorinate: add defluorinating agent in enzymolysis liquid, in defluorinating agent, the mass ratio of calcium acetate and calcium gluconae is 1:0.5, defluorinate condition is: pH9.5, temperature 30 DEG C, reaction time 1h, defluorinating agent addition 0.025g/mL, defluorinate terminates rear interpolation large biological molecule adsorbent, acrylic acid series gel type strong base negative nonionic adsorption resin and macroporous strong-base Anion-adsorption mixed with resin ratio are 1:0.8, adsorption conditions: pH8.0, temperature 35 DEG C, adsorption time 0.5h, adsorbent addition 0.1g/mL, after absorption, enzymolysis liquid is crossed under the condition of 5000g that 100 order filter membranes are centrifugal obtains low fluorine krill enzymolysis liquid.
The defluorinate rate of the present embodiment to krill enzymolysis liquid is 78.4%, and total nitrogen and ammonia nitrogen loss are respectively 2.43%, 3.85%.
embodiment 3
S1. enzymolysis liquid preparation: animal proteolytic enzyme and food flavor enzyme are carried out being mixed to form complex enzyme according to the mass ratio of 1:0.5; After krill cleaning, add deionized water in the ratio of 1:0.5, homogeneous obtains meat gruel, and in meat gruel, add the complex enzyme of 0.1%, slowly stir enzymolysis, enzymatic hydrolysis condition is 45 DEG C, enzymolysis 3h.Go out after enzymolysis completes enzyme 10min at 100 DEG C, and cool to room temperature crosses 60 order filter clothes under the condition of 2000g, and centrifugal 10Min, obtains krill enzymolysis liquid.
S2. defluorinate: add defluorinating agent in enzymolysis liquid, in defluorinating agent, the mass ratio of calcium acetate and calcium gluconae is 1:0.3, defluorinate condition is: pH8.5, temperature 25 DEG C, reaction time 0.5h, defluorinating agent addition 0.005g/mL, defluorinate terminates rear interpolation large biological molecule adsorbent, acrylic acid series gel type strong base negative nonionic adsorption resin and macroporous strong-base Anion-adsorption mixed with resin ratio are 1:0.2, adsorption conditions: pH8.0, temperature 25 DEG C, adsorption time 0.5h, adsorbent addition 0.05g/mL, after absorption, enzymolysis liquid is crossed under the condition of 4000g that 80 order filter membranes are centrifugal obtains low fluorine krill enzymolysis liquid.
The defluorinate rate of the present embodiment to krill enzymolysis liquid is 78.6%, and total nitrogen and ammonia nitrogen loss are respectively 4.38%, 5.12%.
embodiment 4
S1. enzymolysis liquid preparation: animal proteolytic enzyme and food flavor enzyme are carried out being mixed to form complex enzyme according to the mass ratio of 1:0.5; After krill cleaning, add deionized water in the ratio of 1:0.8, homogeneous obtains meat gruel, and in meat gruel, add the complex enzyme of 0.2%, slowly stir enzymolysis, enzymatic hydrolysis condition is 50 DEG C, enzymolysis 3.5h.Go out after enzymolysis completes enzyme 10min at 100 DEG C, and cool to room temperature crosses 200 order filter clothes under the condition of 8000g, and centrifugal 15Min, obtains krill enzymolysis liquid.
S2. defluorinate: add defluorinating agent in enzymolysis liquid, in defluorinating agent, the mass ratio of calcium acetate and calcium gluconae is 1:0.5, defluorinate condition is: pH8.5, temperature 30 DEG C, reaction time 1.5h, defluorinating agent addition 0.03g/mL, defluorinate terminates rear interpolation large biological molecule adsorbent, acrylic acid series gel type strong base negative nonionic adsorption resin and macroporous strong-base Anion-adsorption mixed with resin ratio are 1:0.5, adsorption conditions: pH9.5, temperature 30 DEG C, adsorption time 1h, adsorbent addition 0.1g/mL, after absorption, enzymolysis liquid is crossed under the condition of 5000g that 200 order filter membranes are centrifugal obtains low fluorine krill enzymolysis liquid.
The defluorinate rate of the present embodiment to krill enzymolysis liquid is 80.39%, and total nitrogen and ammonia nitrogen loss are respectively 3.52%, 3.48%.
embodiment 5
S1. enzymolysis liquid preparation: animal proteolytic enzyme and food flavor enzyme are carried out being mixed to form complex enzyme according to the mass ratio of 1:0.8; After krill cleaning, add deionized water in the ratio of 1:1.5, homogeneous obtains meat gruel, and in meat gruel, add the complex enzyme of 0.3%, slowly stir enzymolysis, enzymatic hydrolysis condition is 55 DEG C, enzymolysis 4h.Go out after enzymolysis completes enzyme 10min at 100 DEG C, and cool to room temperature crosses 200 order filter clothes under the condition of 8000g, and centrifugal 20Min, obtains krill enzymolysis liquid.
S2. defluorinate: add defluorinating agent in enzymolysis liquid, in defluorinating agent, the mass ratio of calcium acetate and calcium gluconae is 1:0.6, defluorinate condition is: pH10.0, temperature 35 DEG C, reaction time 2h, defluorinating agent addition 0.03g/mL, defluorinate terminates rear interpolation large biological molecule adsorbent, acrylic acid series gel type strong base negative nonionic adsorption resin and macroporous strong-base Anion-adsorption mixed with resin ratio are 1:0.8, adsorption conditions: pH9.5, temperature 35 DEG C, adsorption time 1h, adsorbent addition 0.15g/mL, after absorption, enzymolysis liquid is crossed under the condition of 7000g that 200 order filter membranes are centrifugal obtains low fluorine krill enzymolysis liquid.
The defluorinate rate of the present embodiment to krill enzymolysis liquid is 84.6%, and total nitrogen and ammonia nitrogen loss are respectively 2.17%, 3.19%.
Claims (7)
1. a preparation method for the low fluorine enzymolysis liquid of krill, is characterized in that, comprise the steps:
S1. utilize the complex enzyme of animal proteolytic enzyme and food flavor enzyme composition to prepare krill enzymolysis liquid, in complex enzyme, the mass ratio of proteolytic enzyme and food flavor enzyme is 1:0.5 ~ 0.8;
S2. utilize organic calcium to carry out chemical method defluorinate process to enzymolysis liquid, described organic calcium is made up of calcium acetate and calcium gluconae, and the mass ratio of calcium acetate and calcium gluconae is 1:0.3 ~ 0.6;
S3. utilize anion exchange resin to adsorb calcium fluoride precipitate and calcium ion, obtain the low fluorine enzymolysis liquid of krill.
2. preparation method according to claim 1, is characterized in that, complex enzyme described in S1 adds according to the mass concentration of 0.1 ~ 0.3%.
3. preparation method according to claim 1, is characterized in that, organic calcium described in S2 adds in enzymolysis liquid according to the addition of 0.005 ~ 0.03g/mL.
4. preparation method according to claim 1, is characterized in that, described anion exchange resin is made up of acrylic acid series gel type strong base negative nonionic adsorption resin and macroporous strong-base Anion-adsorption resin, and the mass ratio of the two is 1:0.2 ~ 0.8.
5. preparation method according to claim 2, it is characterized in that, described step S1 is specially: after krill cleaning, deionized water is added in 1:0.5 ~ 1.5 ratio in shrimp, homogeneous, obtain meat gruel, the addition by 0.1 ~ 0.3% adds complex enzyme in slurries, in 45 ~ 55 DEG C of reaction 3 ~ 4h, the enzyme that goes out after enzymolysis, centrifugally obtain krill enzymolysis liquid.
6. the preparation method of the low fluorine enzymolysis liquid of krill according to claim 3, is characterized in that, before adding organic calcium in S2, controlled enzymatic hydrolysis liquid temp is 25 ~ 35 DEG C, pH 8.5 ~ 10.0, defluorination reaction 0.5 ~ 2h after adding organic calcium.
7. the preparation method of the low fluorine enzymolysis liquid of krill according to claim 3, it is characterized in that, step S3 also comprises pre-treatment step, and being specially control pH is 8.0 ~ 9.5, and temperature is 25 ~ 35 DEG C; Add anion exchange resin after pre-treatment, stirring reaction 0.5 ~ 1h, reaction terminates rear centrifugal, obtains low fluorine krill enzymolysis liquid.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106008661A (en) * | 2016-07-28 | 2016-10-12 | 华南理工大学 | Method for antarctic krill hydrolysatedefluorination |
| CN106472958A (en) * | 2016-09-14 | 2017-03-08 | 安徽农业大学 | A kind of method that efficient selective reduces high fluorine Fluorine in Foods ion |
| CN110699410A (en) * | 2019-09-16 | 2020-01-17 | 浙江海洋大学 | Preparation method of euphausia superba small-molecule peptide |
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