CN105199949B - The fluid control device of gene sequencing - Google Patents
The fluid control device of gene sequencing Download PDFInfo
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- CN105199949B CN105199949B CN201510585327.4A CN201510585327A CN105199949B CN 105199949 B CN105199949 B CN 105199949B CN 201510585327 A CN201510585327 A CN 201510585327A CN 105199949 B CN105199949 B CN 105199949B
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- 238000012163 sequencing technique Methods 0.000 title claims abstract description 308
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 224
- 239000012530 fluid Substances 0.000 title claims abstract description 44
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 202
- 238000006243 chemical reaction Methods 0.000 claims abstract description 23
- 239000007788 liquid Substances 0.000 claims description 65
- 239000000126 substance Substances 0.000 claims description 36
- 239000002699 waste material Substances 0.000 claims description 35
- 238000000605 extraction Methods 0.000 claims description 34
- 239000002994 raw material Substances 0.000 claims description 27
- 239000006226 wash reagent Substances 0.000 claims description 17
- 239000003795 chemical substances by application Substances 0.000 claims description 4
- 230000002068 genetic effect Effects 0.000 abstract description 6
- 239000000306 component Substances 0.000 description 36
- 239000002585 base Substances 0.000 description 34
- 238000005516 engineering process Methods 0.000 description 7
- 239000000284 extract Substances 0.000 description 5
- 206010011409 Cross infection Diseases 0.000 description 3
- 206010029803 Nosocomial infection Diseases 0.000 description 3
- 238000002347 injection Methods 0.000 description 3
- 239000007924 injection Substances 0.000 description 3
- 230000003139 buffering effect Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 101150065751 tps gene Proteins 0.000 description 2
- 101150000874 11 gene Proteins 0.000 description 1
- 235000003140 Panax quinquefolius Nutrition 0.000 description 1
- 240000005373 Panax quinquefolius Species 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 238000005842 biochemical reaction Methods 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 239000008358 core component Substances 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000007672 fourth generation sequencing Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 238000005213 imbibition Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 230000000149 penetrating effect Effects 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 101150012509 sub gene Proteins 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
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Abstract
The present invention discloses a kind of fluid control device of gene sequencing,The fluid control device of the gene sequencing includes reagent component,First multiple-way valve,First triple valve,Gene sequencing chip and drive component,The reagent component is connected through first multiple-way valve and first triple valve with the gene sequencing chip,And utilize setting the first gene sequencing passage and the second gene sequencing passage in the genetic chip,So that the reagent of the reagent component, which can automatically flow into, carries out reaction and fluoroscopic image collection in the first gene sequencing passage and the second gene sequencing passage,And when the first gene sequencing passage carries out fluorescence sequencing reaction,The second gene sequencing passage can carry out fluoroscopic image collection,So as to effectively reduce the gene sequencing time,Effectively increase gene sequencing efficiency,So that the fluid control device of the gene sequencing reduces gene sequencing cost,Improve gene sequencing efficiency.
Description
Technical field
The present invention relates to field of medical device, more particularly to a kind of fluid control device of gene sequencing.
Background technology
Single-molecule sequencing technology has three kinds of technology paths at present：Single molecular fluorescence sequencing technologies, unimolecule synthesize survey in real time
Sequence technology and nano-pore sequencing technology.Single molecular fluorescence sequencing technologies, have the characteristics of accuracy rate is high, the sequencing time is short, single point
The fluid control device of sub- gene sequencing is the key modules of unimolecule tester, and the fluid of unimolecule gene sequencing control dress
Genetic chip in putting is the core component for carrying out biochemical reaction and optical imagery, and the fluid control device of gene sequencing is generally set
Multiple fluid delivery devices are put, the fluid delivery device, which has to genetic chip, conveys various reaction reagents, control reagent flow direction
With flow, control the mixing of reagent, export the functions such as waste liquid.The fluid control device of commercial gene sequencing uses more at present
Extraction or sample introduction of the mode of manipulator sample introduction needle to sequencing reagent, sequencing reagent is time-consuming longer in course of conveying, causes base
It is less efficient because of sequencing, and use cost is higher.
The content of the invention
In view of this, in view of the existing deficiencies of the prior art, its main purpose is to provide a kind of gene that improves and surveyed the present invention
The fluid control device of the gene sequencing of sequence efficiency.
To achieve the above object, the present invention provides a kind of fluid control device of gene sequencing, wherein, the gene sequencing
Fluid control device include reagent component, the first multiple-way valve, the first triple valve, gene sequencing chip and drive component, it is described
Reagent component includes containing the sequencing reagent bottle of gene sequencing reagent, contains the base reagent bottle and Sheng of fluorescence base excision reagent
The fluorometric reagent bottle of dress fluorescence base reagent, first extraction opening of first multiple-way valve including the connection sequencing reagent bottle,
Connect the second extraction opening of the base reagent bottle and be connected to the 3rd extraction opening of the fluorometric reagent bottle, and one goes out liquid
Mouthful, the liquid outlet is connected with first extraction opening or second extraction opening or the 3rd extraction opening, and described the
One triple valve includes liquid sucting port, the first diffluence pass and the second diffluence pass, and the liquid sucting port connects first diffluence pass or described
Second diffluence pass, the liquid sucting port pipeline connect the liquid outlet, and the gene sequencing chip includes the first gene sequencing passage
With the second gene sequencing passage, described in the first gene sequencing passage and the second gene sequencing passage difference pipeline connect
First diffluence pass and second diffluence pass, the drive component include the first syringe pump and the second syringe pump, first note
Penetrate pump and connect the first gene sequencing passage and the second gene sequencing passage with second syringe pump difference pipeline；
Wherein, negative pressure is provided to the first gene sequencing passage in first syringe pump, so that first gene
When sequencing passage obtains the gene sequencing reagent, fluorescence base reagent and fluorometric reagent progress gene sequencing reaction successively, institute
State the second syringe pump to stop providing negative pressure to the second gene sequencing passage, so that the second gene sequencing passage progress is glimmering
Light image gathers.
Wherein, the fluid control device of the gene sequencing also includes sequencing reagent configuration component, and the sequencing reagent is matched somebody with somebody
Putting component includes multiple source chemicals bottles, the second multiple-way valve, the second triple valve and the 3rd syringe pump, multiple source chemicals bottles
To contain a variety of sequencing raw materials, pipeline connects multiple source chemicals bottles to second multiple-way valve simultaneously, and pipeline connects
Second triple valve is connect, second triple valve also pipeline connects the 3rd syringe pump and the sequencing reagent bottle, described
3rd syringe pump is connected through second triple valve and second multiple-way valve with source chemicals bottle one of them described, described
Sequencing reagent bottle is connected through second triple valve and the 3rd syringe pump, wherein, the 3rd syringe pump successively with it is more
The individual source chemicals bottle is connected, and to extract the sequencing raw material in multiple source chemicals bottles, and is mixedly configured into described
Gene sequencing reagent, the 3rd syringe pump are connected with the sequencing reagent bottle, for by the gene sequencing reagent injector
To the sequencing reagent bottle.
Wherein, the first blender, first mixing are connected between second triple valve and the sequencing reagent bottle
Device sets multiple first serpentine tubes, and multiple first serpentine tubes join end to end, and connect in second triple valve and
Between the sequencing reagent bottle.
Wherein, the number of the sequencing reagent bottle is multiple that the sequencing reagent configuration component also includes the 3rd multiple-way valve,
Pipeline connects multiple sequencing reagent bottles, and second triple valve, the 3rd injection to 3rd multiple-way valve simultaneously
Pump is connected through second triple valve and the 3rd multiple-way valve with sequencing reagent bottle one of them described.
Wherein, the sequencing reagent configuration component also includes wash reagent bottle and the first waste liquid bottle, the wash reagent bottle
For containing wash reagent, the wash reagent bottle is through second multiple-way valve and second triple valve and the described 3rd injection
Pump is connected, and first waste liquid bottle is used to contain waste liquid, and first waste liquid bottle is through the 3rd multiple-way valve and described second
Triple valve is connected with the 3rd syringe pump.
Wherein, the fluid control device of the gene sequencing also includes fluorometric reagent configuration component, and the fluorometric reagent is matched somebody with somebody
Putting component includes multiple fluorescence stock bottles, the 4th multiple-way valve, the 3rd triple valve and the 4th syringe pump, multiple fluorescence stock bottles
To contain a variety of fluorescence base raw materials, pipeline connects multiple fluorescence stock bottles, Yi Jiguan to the 4th multiple-way valve simultaneously
Road connects the 3rd triple valve, and the 3rd triple valve also pipeline connects the 4th syringe pump and the fluorometric reagent bottle,
4th syringe pump is connected through the 3rd triple valve and the 4th multiple-way valve with fluorescence stock bottle one of them described,
The fluorometric reagent bottle is connected through the 3rd multiple-way valve with the 4th syringe pump, wherein, the 4th syringe pump is successively
It is connected with multiple fluorescence stock bottles, to extract the fluorescence base raw material in multiple fluorescence stock bottles, and mixes and match somebody with somebody
Fluorescence base reagent is set to, the 4th syringe pump is connected with the fluorometric reagent bottle, for by the fluorescence base reagent
It is injected in the fluorometric reagent bottle.
Wherein, the fluorometric reagent configuration component also includes the second blender, and second blender is connected to described
Between three triple valves and the fluorometric reagent bottle, second blender includes multiple second serpentine tubes, and multiple described second
Serpentine tubes join end to end, and connect between the 3rd triple valve and the fluorometric reagent bottle.
Wherein, the drive component also includes the 4th triple valve, the 5th triple valve, the second waste liquid bottle and the 3rd waste liquid bottle,
The 4th triple valve pipeline is connected between first syringe pump and the first gene sequencing passage, while goes back pipeline company
Second waste liquid bottle is connect, the 5th triple valve pipeline is connected to second syringe pump and the second gene sequencing passage
Between, while go back pipeline and connect the 3rd waste liquid bottle.
Wherein, the number of the first gene sequencing passage and the second gene sequencing passage is multiple.
Wherein, the fluid control device of the gene sequencing also includes control assembly, described in the control assembly electrical connection
First multiple-way valve, the first triple valve, gene sequencing chip and the drive component, to control first multiple-way valve, the one or three
Port valve, gene sequencing chip and drive component operation.
The fluid control device of gene sequencing provided by the invention, by the reagent component through first multiple-way valve and
First triple valve is connected with the gene sequencing chip, and sets first gene to survey using in the genetic chip
Sequence passage and the second gene sequencing passage, so that the reagent of the reagent component can automatically flow into first base
Surveyed because carrying out reaction and fluoroscopic image collection in sequencing passage and the second gene sequencing passage, and in first gene
When sequence passage carries out fluorescence sequencing reaction, the second gene sequencing passage can carry out fluoroscopic image collection, so as to effectively subtract
The gene sequencing time is lacked, that is, has effectively increased gene sequencing efficiency so that the fluid control device of the gene sequencing is reduced
Gene sequencing cost, improve gene sequencing efficiency.
Brief description of the drawings
More clearly to illustrate the construction feature and effect of the present invention, it is entered with specific embodiment below in conjunction with the accompanying drawings
Row describes in detail.
Fig. 1 is the structural representation of the fluid control device of gene sequencing provided by the invention.
Embodiment
Below in conjunction with the accompanying drawing in embodiment of the present invention, the technical scheme in embodiment of the present invention is carried out clear
Chu, it is fully described by.
Also referring to Fig. 1, a kind of fluid control device 100 of gene sequencing provided by the invention.The gene sequencing
Fluid control device 100 include reagent component, the first multiple-way valve 20, the first triple valve 30, gene sequencing chip 40 and driving
Component 50.The reagent component includes containing the sequencing reagent bottle 11 of gene sequencing reagent, contains fluorescence base excision reagent
Base reagent bottle 12 and the fluorometric reagent bottle 13 for containing fluorescence base reagent.First multiple-way valve 20 includes connecting the sequencing
First extraction opening 21 of reagent bottle 11, the connection base reagent bottle 12 the second extraction opening 22 and be connected to the fluorometric reagent
3rd extraction opening 23 of bottle 13, and a sequencing liquid outlet 24.The liquid outlet 24 and first extraction opening 21 or described
Second extraction opening 22 or the 3rd extraction opening 23 are connected.First triple valve 30 includes liquid sucting port 31, the first diffluence pass
32 and second diffluence pass 33, the liquid sucting port 31 connect first diffluence pass 32 or second diffluence pass 33.The imbibition
31 pipelines of mouth connect the liquid outlet 24.The gene sequencing chip 40 includes the first gene sequencing passage 41 and the second gene is surveyed
Sequence passage 42, the first gene sequencing passage 41 connect described first point with the second gene sequencing passage 42 difference pipeline
Head piece 32 and second diffluence pass 33.The drive component 50 includes the first syringe pump 51 and the second syringe pump 52, and described
One syringe pump 51 connects the first gene sequencing passage 41 and second gene with second syringe pump 52 difference pipeline
Passage 42 is sequenced；
Wherein, negative pressure is provided to the first gene sequencing passage 41 in first syringe pump 51, so that described first
Gene sequencing passage 41 obtains the gene sequencing reagent, fluorescence base reagent and fluorometric reagent and carries out gene sequencing reaction successively
When, second syringe pump 52 stops providing negative pressure to the second gene sequencing passage 42, so that second gene sequencing
Passage 42 carries out fluoroscopic image collection.
By the reagent component through first multiple-way valve 20 and first triple valve 30 and the gene sequencing core
Piece 40 is connected, and utilizes setting the first gene sequencing passage 41 and second gene sequencing in the genetic chip 40
Passage 42, so that the reagent of the reagent component can automatically flow into the first gene sequencing passage 41 and described second
Reaction and fluoroscopic image collection are carried out in gene sequencing passage 42, and fluorescence survey is carried out in the first gene sequencing passage 41
When sequence is reacted, the second gene sequencing passage 42 can carry out fluoroscopic image collection, during so as to effectively reduce gene sequencing
Between, that is, effectively increase gene sequencing efficiency so that the fluid control device 100 of the gene sequencing reduce gene sequencing into
This, improves gene sequencing efficiency.
In present embodiment, the opening conduit of the sequencing reagent bottle 11 is connected to first extraction opening 21.The survey
Sequence reagent bottle 11 is made of clear glass test tube, and the opening of the sequencing reagent bottle 11 is arranged at the sequencing reagent bottle 11
Top, opening are plugged with plug (not marking), and plug is inserted with woven hose (not marking), and the woven hose extend into the sequencing examination
The bottom of agent bottle 11, so as to fully extract the gene sequencing reagent of the sequencing reagent bottle 11.In other embodiments,
The sequencing reagent bottle 10 can also be box body.
The base reagent bottle 12 and the fluorometric reagent bottle 13 are identical with the structure of the sequencing reagent bottle 11, herein not
Repeat again.
In present embodiment, first multiple-way valve 20 is rotary valve, first extraction opening 21, the and of the second extraction opening 22
3rd extraction opening 23 is respectively around around the liquid outlet 24, first extraction opening 21, the second extraction opening 22 and institute
State the 3rd extraction opening 23 and the liquid outlet 24 is connected by the rotating pipe 25 rotated around the liquid outlet 24.The rotating pipe
25 can rotate to the position of first extraction opening 21, the second extraction opening 22 and the 3rd extraction opening 23, so as to realize successively
The sequencing reagent bottle 11, the base reagent bottle 12 and the fluorometric reagent bottle 13 can be in turn switched on by stating liquid outlet 24, i.e.,
The gene sequencing chip 30 can be successively from the sequencing reagent bottle 11, the base reagent bottle 12 and the fluorometric reagent bottle
13 obtain reagent, and then gene sequencing reaction and fluoroscopic image collection.In other embodiments, the liquid outlet 24 with it is described
The connection order of first extraction opening 21, the second extraction opening 22 and the 3rd extraction opening 23 can not also limit.
In present embodiment, when the liquid sucting port 31 of first triple valve 30 is connected with first diffluence pass 32, institute
Liquid sucting port 31 is stated to disconnect with second diffluence pass 33.The liquid sucting port 31 can connect described the according to the gene sequencing cycle
One diffluence pass 32 or second diffluence pass 33, i.e., when the first gene sequencing passage 41 carries out gene survey in a cycle
When sequence is reacted, first diffluence pass 32 is connected with the liquid sucting port 30, so as to which the liquid sucting port 30 is through the described first shunting
Mouthfuls 32 provide reaction reagent to the first gene sequencing passage 41, and now second diffluence pass 33 and the liquid sucting port 31
Disconnect, so that the reaction reagent in the second gene sequencing passage 42 stops flowing, so as to realize that fluoroscopic image gathers
Take pictures；After the second gene sequencing passage 42 completes fluoroscopic image collection, second diffluence pass 33 and the liquid sucting port
31 are connected, and the second gene sequencing passage 42 starts to obtain reaction reagent through the liquid sucting port 31, and carries out gene sequencing
Reaction, the first gene sequencing passage 41 then stops obtaining reaction reagent, and carries out fluoroscopic image collection, so as to effectively reduce
The gene sequencing time, improve gene sequencing efficiency.
In present embodiment, the both ends difference pipeline of the first gene sequencing passage 41 connects first triple valve 30
The first diffluence pass 31 and first syringe pump 51.Described in the both ends difference pipeline connection of the second gene sequencing passage 42
The second diffluence pass 32 and second syringe pump 52 of first triple valve 30.Gene is carried out in the first gene sequencing passage 41
Fluoroscopic image collection is carried out during sequencing reaction, in the second gene sequencing passage 42 to take pictures；The second gene sequencing passage
When gene sequencing reaction is carried out in 42, the first gene sequencing passage 41 can carry out fluoroscopic image collection and take pictures, so as to institute
The first gene sequencing passage 41 and the 42 mutually coordinated operation of the second gene sequencing passage are stated, so as to effectively reduce fluorogene
The time is sequenced, so as to improve gene sequencing efficiency.
In present embodiment, first syringe pump 51 and second syringe pump 52 are utilized respectively described in negative pressure extracting
The sequencing waste liquid of gene sequencing reaction has been completed in one gene sequencing passage 41 and the second gene sequencing passage 42, so as to
Ensure that gene sequencing reaction is stable to carry out.
Further, the fluid control device 100 of the gene sequencing also includes sequencing reagent configuration component 60, the survey
Sequence reagent configuration component 60 includes multiple source chemicals bottles 61, the second multiple-way valve 62, the second triple valve 63 and the 3rd syringe pump 64.
To contain a variety of sequencing raw materials, second multiple-way valve 62 while pipeline connect multiple described multiple source chemicals bottles 61
Source chemicals bottle 61, and pipeline connect second triple valve 63.Second triple valve 63 goes back pipeline connection the described 3rd
Syringe pump 64 and the sequencing reagent bottle 11.3rd syringe pump 64 is through second triple valve 63 and second multiple-way valve
62 are connected with source chemicals bottle 61 one of them described.The sequencing reagent bottle 61 is through second triple valve 63 and described the
Three syringe pumps 64 are connected.Wherein, the 3rd syringe pump 64 is connected with multiple source chemicals bottles 61 successively, to extract
Sequencing raw material in multiple source chemicals bottles 61, and the gene sequencing reagent is mixedly configured into, the 3rd syringe pump
64 are connected with the sequencing reagent bottle 11, for by the gene sequencing reagent injector to the sequencing reagent bottle 11.
In present embodiment, multiple source chemicals bottles 61 contain different gene sequencing raw materials respectively, so as to
The gene sequencing raw material in multiple source chemicals bottles 61 is extracted successively using the 3rd syringe pump 64, so as to mixed configuration
Into gene sequencing reagent.Specifically, the number of the source chemicals bottle 61 is ten.The structure of the source chemicals bottle 61 and institute
State the identical setting of structure of sequencing reagent bottle 11.Multiple source chemicals bottles 61 can prevent terminating on rack for test tube, so as to right
Multiple source chemicals bottles 61 are consolidated, while the different marks that can also be sticked respectively to six source chemicals bottles 61
Label, with facilitate carry out next time be sequenced raw material supplemented, avoid be sequenced raw material cross-infection.In other embodiments, institute
The number for stating source chemicals bottle 61 can also be six or eight.
Second multiple-way valve 62 setting identical with the structure of first multiple-way valve 20.Unlike, more than described second
Port valve 62 realizes that the 3rd syringe pump 64 is connected with multiple source chemicals bottles 61 successively, and second multiple-way valve 62 selects
One of them fixed described source chemicals bottle 61 is connected, and connects duration by controlling, so as to control the 3rd syringe pump 64 right
The regulation of sequencing raw material extraction amount in the source chemicals bottle 61.So as to realize that the sequencing raw material of multiple source chemicals bottles 61 can
To be configured in proportion, to meet gene sequencing demand.
Second triple valve 63 setting identical with the structure of the first triple valve 30.Second triple valve 63 can be real
Existing 3rd syringe pump 64 is connected with second multiple-way valve 62, so as to the 3rd syringe pump 64 can extract it is multiple described
Gene sequencing raw material in source chemicals bottle 61, is configured to gene sequencing reagent.Second triple valve 63 can be realized described
3rd syringe pump 64 is connected with the sequencing reagent bottle 11, so as to which the 3rd syringe pump 64 can be to the sequencing reagent bottle 11
It is middle to inject the gene sequencing reagent being configured.
3rd syringe pump 64 can be through second triple valve 63 and second multiple-way valve 62 to multiple originals
Expect that reagent bottle 61 provides negative pressure, to extract the gene sequencing reagent in multiple source chemicals bottles 61.3rd syringe pump
64 can also provide malleation through second triple valve 63 into the sequencing reagent bottle 11, to inject gene sequencing reagent to institute
State in sequencing reagent bottle 11.
Further, the first blender 65, institute are connected between second triple valve 63 and the sequencing reagent bottle 11
State the first blender 65 and multiple first serpentine tubes 651 are set, multiple first serpentine tubes 651 join end to end, and connect
Between second triple valve 63 and the sequencing reagent bottle 11.
In present embodiment, multiple first serpentine tubes 651 are fixed in a fixed plate, first serpentine pipe
Road 651 is S-shaped, and multiple serpentine tubes 651 can be multiple rows of arranged side by side, and mutually be connected between often arranging.Using multiple described
First serpentine tubes 651 are connected between the good sequencing reagent bottle 11 of second triple valve 63 so that from the 3rd note
Penetrate the gene sequencing reagent that pump 64 injects and enter row buffering by multiple serpentine tubes 651, and increase gene sequencing examination
The flowing stroke of agent, so that the several genes sequencing raw material in gene sequencing reagent is sufficiently mixed, lifting gene is surveyed
Sequence reagent reacting efficiency.In other embodiments, multiple serpentine tubes 651 can also be spirals successively.
Further, the number of the sequencing reagent bottle 11 is multiple, and the sequencing reagent configuration component 60 also includes the
Three multiple-way valves 66, pipeline connects multiple sequencing reagent bottles 11, and second threeway to the 3rd multiple-way valve 66 simultaneously
Valve 63, the 3rd syringe pump 64 try through second triple valve 63 and the 3rd multiple-way valve 66 with one of them described sequencing
Agent bottle 11 is connected.
In present embodiment, the sequencing reagent in multiple sequencing reagent bottles 11 is different, the sequencing reagent bottle 11
Number is four.The sequencing source chemicals ratio of multiple source chemicals bottles 61 is extracted not according to the 3rd syringe pump 64
Together, different gene sequencing reagents is can be configured to, so as to contain a variety of differences using multiple sequencing reagent bottles 11
Gene sequencing reagent.The structure of 3rd multiple-way valve 66 setting identical with the structure of the second multiple-way valve 62.More than described 3rd
Port valve 60 can realize that the 3rd syringe pump 64 injects different genes respectively into multiple sequencing reagent bottles 11 successively
Sequencing reagent.Specifically, the 3rd syringe pump 64 is often configured after completing gene sequencing reagent, through the He of the second triple valve 63
3rd multiple-way valve 60 selectes a sequencing reagent bottle 11, and by gene sequencing reagent injector to the sequencing reagent bottle 11
It is interior.In other embodiments, the number of the sequencing reagent bottle 11 can also be five, six or seven.
Further, the sequencing reagent configuration component 60 also includes the waste liquid bottle 68 of wash reagent bottle 67 and first, described
Wash reagent bottle 67 is used to contain wash reagent, and the wash reagent bottle 67 is through second multiple-way valve 63 and second threeway
Valve 62 is connected with the 3rd syringe pump 64, and first waste liquid bottle 68 is used to contain waste liquid, and first waste liquid bottle 68 passes through
3rd multiple-way valve 66 and second triple valve 62 are connected with the 3rd syringe pump 64.
The wash reagent bottle 67 is through second multiple-way valve 63 and second triple valve 62 and the 3rd syringe pump
64 when being connected, and the 3rd syringe pump 64 can extract wash reagent in the wash reagent bottle 67, with to the 3rd note
Penetrate pump 64 to be rinsed, i.e., described 3rd syringe pump 64 is after a kind of gene sequencing reagent is completed in configuration, next time with tps gene
Before sequencing reagent, the wash reagent in the wash reagent bottle 67 can be first extracted, is surveyed again with tps gene after being rinsed
Sequence reagent, there is cross-infection so as to avoid configuring two kinds of different gene sequencing.First waste liquid bottle 68 is through the described 3rd
When multiple-way valve 66 and second triple valve 62 are connected with the 3rd syringe pump 64, the 3rd syringe pump 64 can be by
Waste liquid through completing cleaning is injected in first waste liquid bottle 68, so as to reach the effect of environmental protection recovery.
Further, the fluid control device 100 of the gene sequencing also includes fluorometric reagent configuration component 70, described glimmering
Light reagent configuration component 70 includes multiple fluorescence stock bottles 71, the 4th multiple-way valve 72, the 3rd triple valve 73 and the 4th syringe pump 74.
Multiple fluorescence stock bottles 71 are containing a variety of fluorescence base raw materials.4th multiple-way valve 72 pipeline connection simultaneously is multiple
The fluorescence stock bottle 71, and pipeline connect the 3rd triple valve 73.3rd triple valve 73 is gone back described in pipeline connection
4th syringe pump 74 and the fluorometric reagent bottle 13.4th syringe pump 74 is through the 3rd triple valve 73 and described more than the 4th
Port valve 72 is connected with fluorescence stock bottle 71 one of them described.The fluorometric reagent bottle 13 is through the 4th multiple-way valve 72 and institute
The 4th syringe pump 74 is stated to be connected, wherein, the 4th syringe pump 74 is connected with multiple fluorescence stock bottles 71 successively, with
The fluorescence base raw material in multiple fluorescence stock bottles 71 is extracted, and is mixedly configured into fluorescence base reagent.4th note
Penetrate pump 74 to be connected with the fluorometric reagent bottle 13, for by the fluorescence base reagent injector to the fluorometric reagent bottle 13
It is interior.
In present embodiment, multiple fluorescence stock bottles 71 contain different fluorescence base raw materials respectively, so as to
The gene sequencing raw material in multiple fluorescence stock bottles 71 is extracted successively using the 4th syringe pump 74, so as to mixed configuration
Into fluorescence base reagent.Specifically, the number of the fluorescence stock bottle 71 is five.The structure of the fluorescence stock bottle 71 and institute
State the identical setting of structure of sequencing reagent bottle 11.Multiple fluorescence source chemicals bottles 71 can be disposed on rack for test tube, with
Just multiple fluorescence stock bottles 71 are consolidated, while difference that five fluorescence stock bottles 71 can also be sticked respectively
Label, to facilitate carry out fluorescence source chemicals supplement next time, avoid the cross-infection of fluorescence raw material.In other embodiments,
The number of the fluorescence stock bottle 71 can also be six or eight.
4th multiple-way valve 72 setting identical with the structure of first multiple-way valve 20.Unlike, more than the described 4th
Port valve 72 realizes that the 4th syringe pump 74 is connected with multiple fluorescence stock bottles 71 successively, and the 4th multiple-way valve 72 selects
One of them fixed described fluorescence stock bottle 71 is connected, and connects duration by controlling, so as to control the 4th syringe pump 74 right
The regulation of fluorescence base raw material extraction amount in the fluorescence stock bottle 71.So as to realize the fluorescence alkali of multiple fluorescence stock bottles 71
Based raw material can be configured in proportion, to meet gene sequencing demand.
3rd triple valve 73 setting identical with the structure of the first triple valve 30.3rd triple valve 73 can be real
Existing 4th syringe pump 74 is connected with the 4th multiple-way valve 72, so as to the 4th syringe pump 74 can extract it is multiple described
Fluorescence base raw material in fluorescence stock bottle 71, is configured to fluorescence base reagent.3rd triple valve 73 can be realized described
4th syringe pump 74 is connected with the fluorometric reagent bottle 71, so as to which the 4th syringe pump 74 can be to the fluorometric reagent bottle 13
It is middle to inject the fluorescence base reagent being configured.
4th syringe pump 74 can be through the 3rd triple valve 73 and the 4th multiple-way valve 72 to multiple described glimmering
Light source chemicals bottle 71 provides negative pressure, to extract the gene sequencing reagent in multiple fluorescence stock bottles 71.4th note
Malleation can also be provided through the 3rd triple valve 73 into the fluorometric reagent bottle 71 by penetrating pump 74, to inject gene sequencing reagent
To fluorescence sequencing bottle 71.
Further, the fluorometric reagent configuration component 70 also includes the second blender 75, and second blender 75 connects
It is connected between the 3rd triple valve 73 and the fluorometric reagent bottle 13, second blender 75 includes multiple second serpentine pipes
Road 751, multiple second serpentine tubes 751 join end to end, and connect in the 3rd triple valve 73 and the fluorometric reagent
Between bottle 13.
Second blender 75 setting identical with the structure of the first blender 65, second blender 75 is from described
The fluorescence base reagent that 4th syringe pump 74 injects enters row buffering by multiple second serpentine tubes 751, and increases
The flowing stroke of fluorescence base sequence reagent, so that a variety of fluorescence base raw materials in fluorescence base reagent fully mix
Close, lift gene sequencing reagent reacting efficiency.
Further, the drive component 50 also includes the 4th triple valve 53, the 5th triple valve 54, the and of the second waste liquid bottle 55
3rd waste liquid bottle 56.The pipeline of 4th triple valve 53 is connected to first syringe pump 51 and the first gene sequencing passage
Between 41, while go back pipeline and connect second waste liquid bottle 55.The pipeline of 5th triple valve 54 is connected to second injection
Between pump 52 and the second gene sequencing passage 42, while go back pipeline and connect the 3rd waste liquid bottle 56.
First syringe pump 51 connects the first gene sequencing passage 41 or described through the 4th triple valve 53
Two waste liquid bottles 55, so as to which first syringe pump 51 extracts, to have completed gene sequencing in the first gene sequencing passage 41 anti-
After the waste liquid answered, waste liquid can be injected to second waste liquid bottle 55, so that first syringe pump 51 is carried out next time
Negative pressure is provided to the first gene sequencing passage 41, to carry out gene sequencing reaction.5th triple valve 54 and described the
The identical setting of the structure of four triple valve 53, will not be repeated here, the 3rd waste liquid bottle 56 and second waste liquid bottle 55 the structure phase
With setting, will not be repeated here.
Further, the number of the first gene sequencing passage 41 and the second gene sequencing passage 42 is more
It is individual.It is respectively provided with multiple, can be made using the number of the first gene sequencing passage 41 and the second gene sequencing passage 42
Obtain multiple gene sequencing reactions to carry out simultaneously, so as to further improve gene sequencing efficiency.Same principle, first syringe pump
51 and the number of second syringe pump 52 can also be multiple, multiple first syringe pumps 51 are corresponding to multiple described first
Gene sequencing passage 41 provides negative pressure, and multiple second syringe pumps 52 are corresponding to be carried to multiple second gene sequencing passages 42
For negative pressure.Multiple first syringe pumps 51 and multiple second syringe pumps 52 can be carried out by the way of townhouse braking
Driving, so that multiple first syringe pumps 51 or multiple second syringe pumps 52 can be driven, so as to protect simultaneously
Demonstrate,prove the accuracy of the gene sequencing efficiency.
Further, the fluid control device 100 of the gene sequencing also includes control assembly (not shown), the control
Component electrically connects the first multiple-way valve 20, the first triple valve 30, gene sequencing chip 40 and the drive component 50, with control
First multiple-way valve 20, the first triple valve 30, gene sequencing chip 40 and the drive component 50 are run.The control assembly
It can be single-chip microcomputer, calculator processor or central control processor etc., be led to using control assembly control described more than first
Valve 20, the first triple valve 30, gene sequencing chip 40 and the drive component 50 are run, and realize the fluid control of the gene sequencing
The automatic running of device 100 processed, put on gene side and inhale efficiency.In present embodiment, the control assembly 100 also electrically connects described
Two multiple-way valves 62, the second triple valve 63, the 3rd multiple-way valve 66, the 4th multiple-way valve 72, the 3rd triple valve 73, the and of the 3rd syringe pump 64
4th syringe pump 74 so that the operational efficiency of fluid control device 100 lifting of the gene sequencing.
The fluid control device of gene sequencing provided by the invention, by the reagent component through first multiple-way valve and
First triple valve is connected with the gene sequencing chip, and sets first gene to survey using in the genetic chip
Sequence passage and the second gene sequencing passage, so that the reagent of the reagent component can automatically flow into first base
Surveyed because carrying out reaction and fluoroscopic image collection in sequencing passage and the second gene sequencing passage, and in first gene
When sequence passage carries out fluorescence sequencing reaction, the second gene sequencing passage can carry out fluoroscopic image collection, so as to effectively subtract
The gene sequencing time is lacked, that is, has effectively increased gene sequencing efficiency so that the fluid control device of the gene sequencing is reduced
Gene sequencing cost, improve gene sequencing efficiency.
Described above is the preferred embodiment of the present invention, it is noted that for those skilled in the art
For, under the premise without departing from the principles of the invention, some improvements and modifications can also be made, these improvements and modifications are also considered as
Protection scope of the present invention.
Claims (10)
1. a kind of fluid control device of gene sequencing, it is characterised in that the fluid control device of the gene sequencing includes examination
Agent component, the first multiple-way valve, the first triple valve, gene sequencing chip and drive component, the reagent component include containing gene
The sequencing reagent bottle of sequencing reagent, the base reagent bottle for containing fluorescence base excision reagent and the fluorescence for containing fluorescence base reagent
Reagent bottle, first multiple-way valve include connecting the first extraction opening of the sequencing reagent bottle, connect the base reagent bottle
Second extraction opening and the 3rd extraction opening for being connected to the fluorometric reagent bottle, and a liquid outlet, the liquid outlet with it is described
First extraction opening or second extraction opening or the 3rd extraction opening are connected, first triple valve include liquid sucting port,
First diffluence pass and the second diffluence pass, the liquid sucting port connect first diffluence pass or described second according to the gene sequencing cycle
Diffluence pass, the liquid sucting port pipeline connect the liquid outlet, and the gene sequencing chip includes the first gene sequencing passage and the
Passage is sequenced in two gene, and the first gene sequencing passage connects described first with the second gene sequencing passage difference pipeline
Diffluence pass and second diffluence pass, the drive component include the first syringe pump and the second syringe pump, first syringe pump
The first gene sequencing passage and the second gene sequencing passage are connected with second syringe pump difference pipeline；Wherein,
Negative pressure is provided to the first gene sequencing passage in first syringe pump, so that the first gene sequencing passage obtains successively
When taking the gene sequencing reagent, fluorescence base reagent and fluorometric reagent progress gene sequencing reaction, second syringe pump stops
Negative pressure only is provided to the second gene sequencing passage, so that the second gene sequencing passage carries out fluoroscopic image collection；Institute
Stating the fluid control device of gene sequencing also includes sequencing reagent configuration component, and the sequencing reagent configuration component includes multiple originals
Expect reagent bottle, the second multiple-way valve, the second triple valve and the 3rd syringe pump, the sequencing reagent bottle is through second triple valve and institute
The 3rd syringe pump is stated to be connected, wherein, the 3rd syringe pump is connected with multiple source chemicals bottles successively, more to extract
Sequencing raw material in the individual source chemicals bottle, and it is mixedly configured into the gene sequencing reagent.
2. the fluid control device of gene sequencing according to claim 1, it is characterised in that multiple source chemicals bottles
To contain a variety of sequencing raw materials, pipeline connects multiple source chemicals bottles to second multiple-way valve simultaneously, and pipeline connects
Second triple valve is connect, second triple valve also pipeline connects the 3rd syringe pump and the sequencing reagent bottle, described
3rd syringe pump is connected through second triple valve and second multiple-way valve with source chemicals bottle one of them described, described
3rd syringe pump is connected with the sequencing reagent bottle, for by the gene sequencing reagent injector to the sequencing reagent bottle.
3. the fluid control device of gene sequencing according to claim 2, it is characterised in that second triple valve and institute
State and the first blender is connected between sequencing reagent bottle, first blender sets multiple first serpentine tubes, multiple described
First serpentine tubes join end to end, and connect between second triple valve and the sequencing reagent bottle.
4. the fluid control device of gene sequencing according to claim 2, it is characterised in that the number of the sequencing reagent bottle
Mesh is multiple, and the sequencing reagent configuration component also includes the 3rd multiple-way valve, and the 3rd multiple-way valve pipeline connection simultaneously is multiple
The sequencing reagent bottle, and second triple valve, the 3rd syringe pump is through second triple valve and described more than the 3rd
Port valve is connected with sequencing reagent bottle one of them described.
5. the fluid control device of gene sequencing according to claim 4, it is characterised in that the sequencing reagent configuration group
Part also includes wash reagent bottle and the first waste liquid bottle, and the wash reagent bottle is used to contain wash reagent, the wash reagent bottle
It is connected through second multiple-way valve and second triple valve with the 3rd syringe pump, first waste liquid bottle is used to contain
Waste liquid, first waste liquid bottle are connected through the 3rd multiple-way valve and second triple valve with the 3rd syringe pump.
6. the fluid control device of gene sequencing according to claim 4, it is characterised in that the fluid of the gene sequencing
Control device also includes fluorometric reagent configuration component, and the fluorometric reagent configuration component includes multiple fluorescence stock bottles, more than the 4th
Port valve, the 3rd triple valve and the 4th syringe pump, multiple fluorescence stock bottles are to contain a variety of fluorescence base raw materials, and described
The multiple fluorescence stock bottles of four multiple-way valves pipeline connection simultaneously, and pipeline connection the 3rd triple valve, the described 3rd 3
Port valve also pipeline connects the 4th syringe pump and the fluorometric reagent bottle, the 4th syringe pump through the 3rd triple valve and
4th multiple-way valve is connected with fluorescence stock bottle one of them described, the fluorometric reagent bottle through the 3rd multiple-way valve with
4th syringe pump is connected, wherein, the 4th syringe pump is connected with multiple fluorescence stock bottles successively, to extract
Fluorescence base raw material in multiple fluorescence stock bottles, and be mixedly configured into fluorescence base reagent, the 4th syringe pump with
The fluorometric reagent bottle is connected, for by the fluorescence base reagent injector to the fluorometric reagent bottle.
7. the fluid control device of gene sequencing according to claim 6, it is characterised in that the fluorometric reagent configuration group
Part also includes the second blender, and second blender is connected between the 3rd triple valve and the fluorometric reagent bottle, institute
Stating the second blender includes multiple second serpentine tubes, and multiple second serpentine tubes join end to end, and connects in described
Between three triple valves and the fluorometric reagent bottle.
8. the fluid control device of gene sequencing according to claim 1, it is characterised in that the drive component also includes
4th triple valve, the 5th triple valve, the second waste liquid bottle and the 3rd waste liquid bottle, the 4th triple valve pipeline are connected to described first
Between syringe pump and the first gene sequencing passage, while go back pipeline and connect second waste liquid bottle, the 5th triple valve
Pipeline is connected between second syringe pump and the second gene sequencing passage, while is gone back pipeline and connected the 3rd waste liquid
Bottle.
9. the fluid control device of gene sequencing according to claim 1, it is characterised in that first gene sequencing is led to
The number of road and the second gene sequencing passage is multiple.
10. the fluid control device of gene sequencing according to claim 1, it is characterised in that the stream of the gene sequencing
Member control apparatus also includes control assembly, and the control assembly electrically connects first multiple-way valve, the first triple valve, gene sequencing
Chip and the drive component, to control first multiple-way valve, the first triple valve, gene sequencing chip and the drive component
Operation.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510585327.4A CN105199949B (en) | 2015-09-15 | 2015-09-15 | The fluid control device of gene sequencing |
| PCT/CN2016/098890 WO2017045587A1 (en) | 2015-09-15 | 2016-09-13 | Fluid control device for gene sequencing |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510585327.4A CN105199949B (en) | 2015-09-15 | 2015-09-15 | The fluid control device of gene sequencing |
Publications (2)
| Publication Number | Publication Date |
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| CN105199949A CN105199949A (en) | 2015-12-30 |
| CN105199949B true CN105199949B (en) | 2017-12-12 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510585327.4A Active CN105199949B (en) | 2015-09-15 | 2015-09-15 | The fluid control device of gene sequencing |
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| CN (1) | CN105199949B (en) |
| WO (1) | WO2017045587A1 (en) |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105199949B (en) * | 2015-09-15 | 2017-12-12 | 深圳市瀚海基因生物科技有限公司 | The fluid control device of gene sequencing |
| CN105861293B (en) * | 2016-04-06 | 2017-11-07 | 深圳市瀚海基因生物科技有限公司 | Unimolecule gene sequencer |
| EP3342878B1 (en) * | 2016-12-30 | 2020-07-15 | GeneMind Biosciences Company Limited | Method for controlling base sequence determination reaction, base sequence determination system and control device |
| CN108265112B (en) | 2016-12-30 | 2023-04-25 | 深圳市真迈生物科技有限公司 | Method for controlling sequencing reaction, sequencing system and control device |
| CN108456748B (en) * | 2017-02-22 | 2023-04-25 | 深圳市真迈生物科技有限公司 | Method, device and system for controlling sequence determination reaction |
| CN108949939B (en) * | 2017-05-26 | 2023-04-25 | 深圳市真迈生物科技有限公司 | Method, device and system for controlling sequence determination reaction |
| CN107828641A (en) * | 2017-08-23 | 2018-03-23 | 苏州思维医疗科技有限公司 | A kind of gene sequencer |
| CN112326652B (en) * | 2017-09-20 | 2021-09-03 | 深圳市真迈生物科技有限公司 | Imaging method, device and system for controlling sequence determination reaction |
| CN111040942B (en) * | 2019-12-05 | 2023-06-27 | 深圳清华大学研究院 | Gene sequencing device and gene sequencing method |
| CN110904206B (en) * | 2019-12-18 | 2024-10-15 | 深圳市真迈生物科技有限公司 | Liquid path system, biological molecule analysis system and nucleic acid sequence determination system |
| CN110967318A (en) * | 2019-12-23 | 2020-04-07 | 杭州纽蓝科技有限公司 | Detection channel, sample introduction system, single channel and series sample introduction method of SPR chip |
| CN114250144B (en) * | 2021-12-17 | 2024-08-20 | 深圳铭毅智造科技有限公司 | Liquid path system for gene sequencing |
| CN114231403A (en) * | 2022-01-13 | 2022-03-25 | 深圳清华大学研究院 | Gene sequencer |
| CN117343832A (en) * | 2023-10-10 | 2024-01-05 | 中元汇吉生物技术股份有限公司 | Reciprocating gene sequencing system and sequencing method |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH08500052A (en) * | 1992-07-06 | 1996-01-09 | ベックマン インスツルメンツ インコーポレーテッド | Fluid delivery device using multi-port valve |
| US6818395B1 (en) * | 1999-06-28 | 2004-11-16 | California Institute Of Technology | Methods and apparatus for analyzing polynucleotide sequences |
| JP3754038B2 (en) * | 2003-07-22 | 2006-03-08 | 株式会社東芝 | Flow control device and flow control system |
| CN102703312B (en) * | 2012-05-24 | 2014-03-19 | 中国科学院北京基因组研究所 | DNA (Deoxyribose Nucleic Acid) sequencer |
| CA3009218C (en) * | 2013-08-08 | 2020-10-27 | Illumina, Inc. | Fluidic system for reagent delivery to a flow cell |
| CN204874555U (en) * | 2015-08-14 | 2015-12-16 | 深圳市瀚海基因生物科技有限公司 | Fluid controlling means of gene sequencing |
| CN105199949B (en) * | 2015-09-15 | 2017-12-12 | 深圳市瀚海基因生物科技有限公司 | The fluid control device of gene sequencing |
| CN105861293B (en) * | 2016-04-06 | 2017-11-07 | 深圳市瀚海基因生物科技有限公司 | Unimolecule gene sequencer |
| CN205576142U (en) * | 2016-04-06 | 2016-09-14 | 深圳市瀚海基因生物科技有限公司 | Unimolecule gene sequencer |
-
2015
- 2015-09-15 CN CN201510585327.4A patent/CN105199949B/en active Active
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2016
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| WO2017045587A1 (en) | 2017-03-23 |
| CN105199949A (en) | 2015-12-30 |
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