CN105145957A - Health herbal tea for treating oral ulcer, preparation method of health herbal tea and detection method - Google Patents
Health herbal tea for treating oral ulcer, preparation method of health herbal tea and detection method Download PDFInfo
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Abstract
The invention belongs to the technical field of food processing, and particularly relates to health herbal tea for treating oral ulcer, a preparation method of the health herbal tea and a detection method. The herbal tea is health herbal tea and is prepared from the following raw materials in parts by weight: 5-10 parts of green tea, 1-5 parts of cymbopon citratus, 1-5 parts of rosa bella flower, 1-5 parts of biondia henryi, 1-5 parts of rhoeo discolor and 1-5 parts of epipactis helleborine root. The preparation method includes the following steps: taking cymbopon citratus, biondia henryi and epipactis helleborine root and decocting with water three times with one hour each time; combining the decoction solutions, filtering, concentrating the filtrate to obtain thick paste with the density of 1.25; taking rosa bella flower and rhoeo discolor, crushing into coarse powder, mixing uniformly with the thick paste, drying, adding the green tea, mixing uniformly, sieving, packaging with teabag used filter paper bags and sealing.
Description
Technical field
The invention belongs to food processing technology field, relate to a kind of health drink, be specially a kind of health protection tea canker sore being had to certain therapeutic action.
Background technology
Recurrent oral ulceration is also known as recurrent aphthous ulcer (recurrentaphthousulcer, RAU), and recurrent oral ulceration, be modal oral mucosal disease, its incidence of disease is up to about 20%.The lighter's several months recurs once, the chronic delay of severe one, recurrent exerbation, hinders patient diet or speaks, making it feel very painful.The RAU cause of disease cause of disease is still not clear, and there is obvious individual difference, at present clinically without effective methods for the treatment of. domestic and international many scholars have carried out large quantity research to the pathogenesis of RAU, think that recurrent oral ulceration is the ulcer disease that immunologic dysfunction causes, wherein oxygen free radical injury, microcirculation disorder and microelement deficiencies may play an important role in the generation evolution of RAU.
Although the pathogenesis of modern medicine to RAU there has been darker understanding, because its cause of disease still can not be completely clear and definite, therefore clinically at present there is no desirable methods for the treatment of, some its effects of traditional methods for the treatment of are also unsatisfactory.In recent years, inventor adopts health tea of the present invention to be used for the treatment of RAU, achieves good curative effect.
In the present invention:
Lemongrass is grass family Cymbopogon plants lemongrass
cymbopogoncitratus (DC.) Stapfflower.
Mei Qiangwie flower is rose family gul Mei Qiangwie
rosabellaRehd.etWilsflower.
Henry biondia herb with root is Asclepiadaceae biondia hennriyi platymiscium biondia hennriyi
biondiahenryi (Warb.exSchltr.etDiels) TsiangetP.T.Li [CynanchumhenryiWarb.exSchltr.etDiels]whole herb with root.
Oyster plant inflorescence is that Commelianaceae Rhoeo plant purple is evergreen
rhoeodiscolor (L ' herit.) Hanceflower.
Smallflower epipactis root is the orchid family helleborine platymiscium little Hua helleborine
epipactishelleborine (L.) Crantz [SerapiashelleborineL.; S.latifolia (L.) All.]root.
Summary of the invention
The object of this invention is to provide a kind of health drink with treatment canker sore effect, this health drink is health protection tea.
Another object of the present invention is to provide the preparation method of this health protection tea.
The object of the invention is to realize in the following manner:
Have the health drink for the treatment of canker sore effect, this health drink is health protection tea, is to be made up of the raw material of following weight parts: green tea 5 ~ 10 parts, 1 ~ 5 part, lemongrass, Mei Qiangwie spends 1 ~ 5 part, henry biondia herb with root 1 ~ 5 part, oyster plant inflorescence 1 ~ 5 part, smallflower epipactis root 1 ~ 5 part.
Described health drink is made up of the raw material of following weight parts: green tea 10 parts, 5 parts, lemongrass, and Mei Qiangwie spends 5 parts, henry biondia herb with root 5 parts, oyster plant inflorescence 4 parts, smallflower epipactis root 4 parts.
Described health drink can also be made up of the raw material of following weight parts: green tea 10 parts, 2 parts, lemongrass, and Mei Qiangwie spends 3 parts, henry biondia herb with root 4 parts, oyster plant inflorescence 5 parts, smallflower epipactis root 4 parts.
Above-mentioned health drink is made up of following method: get lemongrass, Mei Qiangwie flower, henry biondia herb with root, oyster plant inflorescence, smallflower epipactis root, dry, and pulverizes, mixes with green tea, adopts conventional method to make loose tea, compressed tea.
Above-mentioned health drink can also be made up of following method: get lemongrass, Mei Qiangwie flower, henry biondia herb with root, oyster plant inflorescence, smallflower epipactis root, dry, and pulverizes, mixes with green tea, adopts conventional method to make loose tea, compressed tea, tea in bag.
Above-mentioned health drink can also be made up of following method: get lemongrass, henry biondia herb with root, smallflower epipactis root boiling 3 times, each 1 hour, collecting decoction, and filter, filtrate is concentrated into the thick paste that suitable density is 1.25; Get Mei Qiangwie flower, oyster plant inflorescence is ground into meal, mix with thick paste, dry, add green tea, mixing, sieves, and loads in tea in bag filter paper bag, sealing, and every bag weighs 2g.
Health drink of the present invention can adopt following method to carry out quality testing:
Adopt high effective liquid chromatography for measuring verbascoside (verbascoside) content:
(1) chromatographic condition: chromatographic column is C
18chromatographic column; Be that the methyl alcohol-0.2mol/L sodium dihydrogen phosphate of 40 ~ 60:40 ~ 60 is for mobile phase with ratio; Determined wavelength 300 ~ 330nm, column temperature 25 ~ 45 DEG C;
(2) preparation of reference substance solution: precision takes verbascoside reference substance and is placed in volumetric flask, adds methyl alcohol and dissolves and be diluted to scale, shake up; Pipette above-mentioned solution and put volumetric flask, add methanol constant volume, in contrast product stock solution;
(3) preparation of need testing solution: get health protection tea of the present invention, precision weighing, put in tool plug conical flask, precision adds methyl alcohol, close plug, weighed weight, ultrasonic process, lets cool, more weighed weight, supplies the weight of less loss with methyl alcohol, shake up, filtering with microporous membrane, get subsequent filtrate, obtain need testing solution;
(4) measure: precision measures above-mentioned need testing solution, each 5 ~ 20 μ L of reference substance solution respectively, inject high performance liquid chromatograph, detect.
Health drink of the present invention preferably adopts following method to carry out quality testing:
Adopt high effective liquid chromatography for measuring verbascoside content:
(1) chromatographic condition: chromatographic column is C
18chromatographic column; Take ratio as the methyl alcohol-0.2mol/L sodium dihydrogen phosphate of 40:60 be mobile phase; Determined wavelength 312nm, column temperature 35 DEG C; Number of theoretical plate calculates should be not less than 4000 by verbascoside peak;
(2) preparation of reference substance solution: precision takes verbascoside reference substance 10.00mg and is placed in 100ml volumetric flask, adds methyl alcohol and dissolves and be diluted to scale, shake up; Pipette above-mentioned solution 10ml and put 25ml volumetric flask, add methanol constant volume, in contrast product stock solution;
(3) preparation of need testing solution: get health protection tea 3g of the present invention, precision weighing, puts in tool plug conical flask, precision adds methyl alcohol 20ml, close plug, weighed weight, ultrasonic process 30min, let cool, more weighed weight, the weight of less loss is supplied with methyl alcohol, shake up, 0.45 μm of filtering with microporous membrane, gets subsequent filtrate, obtains need testing solution;
(4) measure: precision measures above-mentioned need testing solution, each 10 μ L of reference substance solution respectively, inject high performance liquid chromatograph, detect.
This health drink is for the preparation of the purposes in treatment canker sore, gingivitis, periodontitis health drink.
Experiment one: the effect observation of health protection tea treatment canker sore of the present invention
1 general clinical data
1.1 ordinary circumstances:
In the routine outpatient of this disease 60, the male sex 30 example, women 30 example;
Age of onset was at 30 ~ 50 years old, and 30 is routine; 50 ~ 60 years old, 18 examples; 60 ~ 70 years old, 12 examples;
Course of disease January ~ March, 29 examples; April ~ May, 21 examples; More than half a year, 10 examples.
60 routine patients are divided into conventional group and observation group at random:
Observation group:
Totally 30 examples, the male sex 15 example, women 15 example.
Age of onset was at 30 ~ 50 years old, and 15 is routine; 50 ~ 60 years old, 10 examples; 60 ~ 70 years old, 5 examples.
Course of disease January ~ March, 14 examples; April ~ May, 10 examples; More than half a year, 6 examples.
Conventional group:
Totally 30 examples, the male sex 15 example, women 15 example.
Age of onset was at 30 ~ 50 years old, and 15 is routine; 50 ~ 60 years old, 8 examples; 60 ~ 70 years old, 7 examples.
Course of disease January ~ March, 15 examples; April ~ May, 11 examples; More than half a year, 4 examples.
Two groups of patients in age, sex and other drug treatment without significant difference.
1.2 case selections: oral mucosa single or multiple diameter occurs in 3 ~ 5 millimeter or larger circle, ellipse comparatively shows shallow ulcer, and skin surface has secretion to adhere to. and mostly congested around, with scorching hot, pain, inconvenience of speaking, eating difficulties, even there is constitutional symptom in salivation.Ulcer recurrent exerbation, the very micro-person of result for the treatment of.Get rid of Behcet's disease, carcinous or tuberculous ulcer.
1.3 methods for the treatment of
The patient of observation group, adopts health protection tea of the present invention to be taken after being infused in hot water or decoction and frequently drinks.5 days 1 courses for the treatment of, namely general 1 ~ 2 course for the treatment of stops, any other medicines of stopping using during this period.
The patient of conventional group, adopts green tea to be taken after being infused in hot water or decoction and frequently drinks.5 days 1 courses for the treatment of, namely general 1 ~ 2 course for the treatment of stops, any other medicines of stopping using during this period.
1.4 canker sore diagnostic criteria
Cure: clinical symptom disappearance, canker sore heals, and follows up a case by regular visits in 1 year and has no recidivist;
Effective: clinical symptom relief, canker sore heals substantially;
Take a turn for the better: ulcer recurrence time lengthening after treatment, the state of an illness alleviates, and ulcer obviously reduces;
Invalid: after drug withdrawal, namely ulcer recur to some extent.
1.5 statistical method
Adopt SPSS13.0 statistical software, continuous data is used (
± s) represent, adopt t inspection, enumeration data adopts χ
2inspection, inspection level α=0.05.
1.6 treatment results:
Treatment results is in Table 1-1.
Canker sore comparitive study after table 1-1 treatment
Note: the conventional group of observation group vs, P < 0.05.
Drink health protection tea of the present invention, in 3 days, cure 17 examples, cure 12 in 7 ~ 10 days and fall.Generally take effect at 2 ~ 5 days.
6 conclusions
Health protection tea tea of the present invention can strengthen immunity of organisms, all has good inhibitory action to staphylococcus aureus, Escherichia coli, pneumococcus, beta streptococcus.The pH value of health protection tea of the present invention is 6 ~ 7, substantially equal with the pH value in oral cavity, and drink the growth that health protection tea of the present invention is conducive to Oral normal flora, have good trophism to the surface of a wound, lasting use obviously can treat canker sore, increases mouth comfort.Therefore, health protection tea of the present invention is a kind of economy, effective, the safe health beverages for the treatment of canker sore.
2 model cases
Zhang, the male sex, 35 years old, certain enterprise office civilian, first visit on June 4 in 2015.Recurrent exerbation canker sore more than 2 year, tongue and oral mucosa has multiple ulcer differed in size, attaches most importance to left side, hurt like hell, eating difficulties, speech inconvenience, and dizzy weak, body is become thin, feverishness in palms and soles, uneasy sleeping at night.1 wheat harvesting period is gone through in this time outbreak, and patient is very painful, and through traditional Chinese and western medicine multi-treatment, poor effect, comes to go to a doctor upon somebody's introduction, red tongue body, the thin micro-Huang of tongue, the thin string of arteries and veins.Distinguish as yin asthenia generating intrinsic heat, control with clearing heat and nourishing yin, namely give health protection tea of the present invention and be taken after being infused in hot water or decoction, join 5 bags altogether, every day 1 wraps, and advise drink frequently, namely 4 bags that are only taken after being infused in hot water or decoction fully recover, and so far there are no recurs, good results.
experiment two: the quality standard research of health protection tea of the present invention
Adopt verbascoside content in high effective liquid chromatography for measuring health protection tea of the present invention
The preparation of 1 sample solution
The preparation of 1.1 reference substance solution
Precision takes verbascoside reference substance 10.00mg and is placed in 100ml volumetric flask, adds methyl alcohol and dissolves and be diluted to scale, shake up.Pipette above-mentioned solution 10ml and put 25ml volumetric flask, add methanol constant volume, in contrast product stock solution (verbascoside 40.80 μ g/ml).
The preparation of 1.2 need testing solutions
Prescription: green tea 30 kilograms, 15 kilograms, lemongrass, Mei Qiangwie spends 15 kilograms, henry biondia herb with root 15 kilograms, oyster plant inflorescence 12 kilograms, smallflower epipactis root 12 kilograms;
Method for making: get lemongrass, henry biondia herb with root, smallflower epipactis root boiling 3 times, each 1 hour, collecting decoction, filter, filtrate is concentrated into the thick paste that suitable density is 1.25; Get Mei Qiangwie flower, oyster plant inflorescence is ground into meal, mix with thick paste, dry, add green tea, mixing, sieves, and employing conventional method makes tea in bag, every pouch 2 grams.
Get health protection tea 3g of the present invention, precision weighing, put in tool plug conical flask, precision adds methyl alcohol 20ml, close plug, weighed weight, ultrasonic process 30min, lets cool, more weighed weight, supplies the weight of less loss with methyl alcohol, shake up, 0.45 μm of filtering with microporous membrane, gets subsequent filtrate, obtains need testing solution.
The preparation of 1.3 negative controls
All the other compositions got in prescription composition except lemongrass are made not containing the negative controls of lemongrass, by " 1.2 " obtained negative control solution.Go out peak position at verbascoside and do not occur absworption peak, illustrate that the assay of other compositions to verbascoside in health protection tea of the present invention does not disturb.
2 chromatographic conditions
Chromatographic column is SHIMADZUC
18chromatographic column (5 μm, 4.6mm × 150.0mm, Shimadzu Corporation); With methyl alcohol-0.2mol/L sodium dihydrogen phosphate (40:60) for mobile phase; Determined wavelength 312nm, column temperature 35 DEG C.Number of theoretical plate calculates should be not less than 4000 by verbascoside peak; With this understanding, in health protection tea of the present invention, verbascoside is separated good.
3 methodological studies.
3.1 the range of linearity
The above-mentioned verbascoside reference substance solution of accurate absorption, adds methanol dilution and becomes verbascoside concentration to be respectively the solution of 0.816,1.632,3.264,4.896,6.528,8.160 μ g/ml, sample introduction 10 μ l successively.With peak area (Y) for ordinate, carry out linear regression with sample introduction concentration (X) for abscissa, obtain regression equation:
Y=3.638 × 104X-1.068 × 104, correlation coefficient r=0.9996.Show that verbascoside concentration is good linear relation with chromatographic peak area within the scope of 0.816 ~ 8.160 μ g/ml.
3.2 precision test
Get the verbascoside reference substance solution 10 μ l METHOD FOR CONTINUOUS DETERMINATION 5 times that concentration is 4.890 μ g/ml, RSD is 0.15%, shows that instrument precision is good.
3.3 stability test
Get same need testing solution 0,2,4,6,8h sample introduction 10 μ l respectively, record peak area, result shows: need testing solution is placed in 8h stable in room temperature, and verbascoside peak area RSD is 1.60%.
3.4 replica test
By health protection tea of the present invention, parallel extraction prepares 6 parts of need testing solutions, and sample introduction measures respectively, and RSD is 1.23%(n=6), show that the method repeatability is good.
3.5 average recovery tests
Precision takes each 9 parts of the health protection tea of the present invention of known content, adds the verbascoside reference substance of respective amount, by need testing solution preparation method's process and measure.Average recovery result of the test is in Table 2-1.Verbascoside average recovery rate is 97.7%, RSD is 1.66%, shows that the method accurately, reliably.
Table 2-1 recovery test result (n=9)
3.6 sample sizes measure.Get 4 batches, health protection tea sample of the present invention, according to method prepared by need testing solution, sample introduction after 0.45 μm of filtering with microporous membrane, different lot number health protection tea content of the present invention is in Table 2-2.
Table 2-2 sample determination result (n=3)
detailed description of the invention :
embodiment 1:
Prescription: green tea 10 kilograms, 5 kilograms, lemongrass, Mei Qiangwie spends 5 kilograms, henry biondia herb with root 5 kilograms, oyster plant inflorescence 4 kilograms, smallflower epipactis root 4 kilograms.
Get above-mentioned lemongrass, Mei Qiangwie flower, henry biondia herb with root, oyster plant inflorescence, smallflower epipactis root, dry, be crushed to 20 orders, mix with green tea, adopt conventional method to make tea in bag, every pouch 2 grams.
embodiment 2:
Prescription: green tea 10 kilograms, 2 kilograms, lemongrass, Mei Qiangwie spends 3 kilograms, henry biondia herb with root 4 kilograms, oyster plant inflorescence 5 kilograms, smallflower epipactis root 4 kilograms.
Method for making: get above-mentioned lemongrass, Mei Qiangwie flower, henry biondia herb with root, oyster plant inflorescence, smallflower epipactis root, dry, pulverizes, mixes with green tea, adopts conventional method to make compressed tea.
embodiment 3:
Prescription: green tea 5 kilograms, 4 kilograms, lemongrass, Mei Qiangwie spends 5 kilograms, henry biondia herb with root 5 kilograms, oyster plant inflorescence 1 kilogram, smallflower epipactis root 5 kilograms.
Method for making: get above-mentioned lemongrass, Mei Qiangwie flower, henry biondia herb with root, oyster plant inflorescence, smallflower epipactis root, dry, pulverizes, mixes with green tea, adopts conventional method to make loose tea.
embodiment 4:
Prescription: green tea 30 kilograms, 15 kilograms, lemongrass, Mei Qiangwie spends 15 kilograms, henry biondia herb with root 15 kilograms, oyster plant inflorescence 12 kilograms, smallflower epipactis root 12 kilograms;
Method for making: get lemongrass, henry biondia herb with root, smallflower epipactis root boiling 3 times, each 1 hour, collecting decoction, filter, filtrate is concentrated into the thick paste that suitable density is 1.25; Get Mei Qiangwie flower, oyster plant inflorescence is ground into meal, mix with thick paste, dry, add green tea, mixing, sieves, and employing conventional method makes tea in bag, every pouch 2 grams.
Detection method: adopt high effective liquid chromatography for measuring verbascoside content:
(1) chromatographic condition: chromatographic column is SHIMADZUC
18chromatographic column; Take ratio as the methyl alcohol-0.2mol/L sodium dihydrogen phosphate of 40:60 be mobile phase; Determined wavelength 312nm, column temperature 35 DEG C; Number of theoretical plate calculates should be not less than 4000 by verbascoside peak;
(2) preparation of reference substance solution: precision takes verbascoside reference substance 10.00mg and is placed in 100ml volumetric flask, adds methyl alcohol and dissolves and be diluted to scale, shake up; Pipette above-mentioned solution 10ml and put 25ml volumetric flask, add methanol constant volume, in contrast product stock solution;
(3) preparation of need testing solution: get health protection tea 3g of the present invention, precision weighing, puts in tool plug conical flask, precision adds methyl alcohol 20ml, close plug, weighed weight, ultrasonic process 30min, let cool, more weighed weight, the weight of less loss is supplied with methyl alcohol, shake up, 0.45 μm of filtering with microporous membrane, gets subsequent filtrate, obtains need testing solution;
(4) measure: precision measures above-mentioned need testing solution, each 10 μ L of reference substance solution respectively, inject high performance liquid chromatograph, detect;
(5) result: in every gram of health protection tea of the present invention, verbascoside content is 50.73 μ g.
In addition, be to be understood that, although this description is described according to embodiment, but not each embodiment only comprises an independently technical scheme, this narrating mode of description is only for clarity sake, those skilled in the art should by description integrally, and the technical scheme in each embodiment also through appropriately combined, can form other embodiments that it will be appreciated by those skilled in the art that.
Claims (9)
1. treating a health drink for canker sore, it is characterized in that this health drink is health protection tea, is be made up of the raw material of following weight parts: green tea 5 ~ 10 parts, 1 ~ 5 part, lemongrass, Mei Qiangwie spends 1 ~ 5 part, henry biondia herb with root 1 ~ 5 part, oyster plant inflorescence 1 ~ 5 part, smallflower epipactis root 1 ~ 5 part.
2. health drink as claimed in claim 1, it is characterized in that this health drink is made up of the raw material of following weight parts: green tea 10 parts, 5 parts, lemongrass, Mei Qiangwie spends 5 parts, henry biondia herb with root 5 parts, oyster plant inflorescence 4 parts, smallflower epipactis root 4 parts.
3. the health drink as described in claim 1 or 2 any one, it is characterized in that this health drink is made up of following method: get lemongrass, Mei Qiangwie flower, henry biondia herb with root, oyster plant inflorescence, smallflower epipactis root, dry, pulverize, mix with green tea, adopt conventional method to make loose tea, compressed tea.
4. the health drink as described in claim 1 or 2 any one, it is characterized in that this health drink is made up of following method: get lemongrass, henry biondia herb with root, smallflower epipactis root boiling 1 ~ 4 time, each 0.5 ~ 2 hour, collecting decoction, filter, filtrate is concentrated into the thick paste that suitable density is 1.10 ~ 1.30; Get Mei Qiangwie flower, oyster plant inflorescence is ground into meal, mix with thick paste, dry, add green tea, mixing, sieves, in loading tea in bag filter paper bag.
5. health drink as claimed in claim 4, is characterized in that this health drink is made up of following method: get lemongrass, henry biondia herb with root, smallflower epipactis root boiling 3 times, each 1 hour, collecting decoction, filtration, filtrate is concentrated into the thick paste that suitable density is 1.25; Get Mei Qiangwie flower, oyster plant inflorescence is ground into meal, mix with thick paste, dry, add green tea, mixing, sieves, in loading tea in bag filter paper bag.
6. the health drink as described in claim 1 or 2 any one, is characterized in that this health drink adopts following method to carry out quality testing: adopt high effective liquid chromatography for measuring verbascoside content:
(1) chromatographic condition: chromatographic column is C
18chromatographic column; Be that the methyl alcohol-0.2mol/L sodium dihydrogen phosphate of 40 ~ 60:40 ~ 60 is for mobile phase with ratio; Determined wavelength 300 ~ 330nm, column temperature 25 ~ 45 DEG C;
(2) preparation of reference substance solution: precision takes verbascoside reference substance and is placed in volumetric flask, adds methyl alcohol and dissolves and be diluted to scale, shake up; Pipette above-mentioned solution and put volumetric flask, add methanol constant volume, in contrast product stock solution;
(3) preparation of need testing solution: get health protection tea of the present invention, precision weighing, put in tool plug conical flask, precision adds methyl alcohol, close plug, weighed weight, ultrasonic process, lets cool, more weighed weight, supplies the weight of less loss with methyl alcohol, shake up, filtering with microporous membrane, get subsequent filtrate, obtain need testing solution;
(4) measure: precision measures above-mentioned need testing solution, each 5 ~ 20 μ L of reference substance solution respectively, inject high performance liquid chromatograph, detect.
7. the health drink as described in claim 6 any one, is characterized in that this health drink adopts following method to carry out quality testing: adopt high effective liquid chromatography for measuring verbascoside content:
(1) chromatographic condition: chromatographic column is C
18chromatographic column; Take ratio as the methyl alcohol-0.2mol/L sodium dihydrogen phosphate of 40:60 be mobile phase; Determined wavelength 312nm, column temperature 35 DEG C; Number of theoretical plate calculates should be not less than 4000 by verbascoside peak;
(2) preparation of reference substance solution: precision takes verbascoside reference substance 10.00mg and is placed in 100ml volumetric flask, adds methyl alcohol and dissolves and be diluted to scale, shake up; Pipette above-mentioned solution 10ml and put 25ml volumetric flask, add methanol constant volume, in contrast product stock solution;
(3) preparation of need testing solution: get health protection tea 3g of the present invention, precision weighing, puts in tool plug conical flask, precision adds methyl alcohol 20ml, close plug, weighed weight, ultrasonic process 30min, let cool, more weighed weight, the weight of less loss is supplied with methyl alcohol, shake up, 0.45 μm of filtering with microporous membrane, gets subsequent filtrate, obtains need testing solution;
(4) measure: precision measures above-mentioned need testing solution, each 10 μ L of reference substance solution respectively, inject high performance liquid chromatograph, detect.
8. the health drink as described in claim 1 or 2 any one is for the preparation of the purposes for the treatment of in gingivitis health drink.
9. the health drink as described in claim 1 or 2 any one is for the preparation of the purposes controlled in periodontitis health drink.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106071506A (en) * | 2016-08-25 | 2016-11-09 | 陈伊芸 | A kind of special antipyretic beverage of oral ulcer and preparation method thereof |
| CN106552097A (en) * | 2016-11-29 | 2017-04-05 | 广西大学 | A kind of health protection tea for treating canker sore |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102726558A (en) * | 2011-04-10 | 2012-10-17 | 董爱香 | Health care green tea |
| CN103141631A (en) * | 2013-02-20 | 2013-06-12 | 蓝照斓 | Japanese raspberry root health-care tea |
| CN103520502A (en) * | 2013-10-18 | 2014-01-22 | 李佃场 | Chinese medicinal preparation for treating gingivitis and preparation method thereof |
-
2015
- 2015-10-08 CN CN201510643609.5A patent/CN105145957B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102726558A (en) * | 2011-04-10 | 2012-10-17 | 董爱香 | Health care green tea |
| CN103141631A (en) * | 2013-02-20 | 2013-06-12 | 蓝照斓 | Japanese raspberry root health-care tea |
| CN103520502A (en) * | 2013-10-18 | 2014-01-22 | 李佃场 | Chinese medicinal preparation for treating gingivitis and preparation method thereof |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106071506A (en) * | 2016-08-25 | 2016-11-09 | 陈伊芸 | A kind of special antipyretic beverage of oral ulcer and preparation method thereof |
| CN106552097A (en) * | 2016-11-29 | 2017-04-05 | 广西大学 | A kind of health protection tea for treating canker sore |
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