CN105128345A - Microporous container and manufacture method thereof - Google Patents
Microporous container and manufacture method thereof Download PDFInfo
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- CN105128345A CN105128345A CN201510486267.0A CN201510486267A CN105128345A CN 105128345 A CN105128345 A CN 105128345A CN 201510486267 A CN201510486267 A CN 201510486267A CN 105128345 A CN105128345 A CN 105128345A
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- microporous hollow
- hollow cylinder
- film
- microporous
- actuated
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- 238000000034 method Methods 0.000 title claims abstract description 12
- 238000004519 manufacturing process Methods 0.000 title abstract description 3
- 239000000376 reactant Substances 0.000 claims abstract description 19
- 239000010408 film Substances 0.000 claims description 79
- 239000000463 material Substances 0.000 claims description 20
- 239000004033 plastic Substances 0.000 claims description 15
- 238000002844 melting Methods 0.000 claims description 14
- 230000008018 melting Effects 0.000 claims description 11
- 238000002360 preparation method Methods 0.000 claims description 11
- 239000004568 cement Substances 0.000 claims description 6
- 150000001875 compounds Chemical class 0.000 claims description 6
- 239000010409 thin film Substances 0.000 claims description 6
- 239000011248 coating agent Substances 0.000 claims description 3
- 238000000576 coating method Methods 0.000 claims description 3
- 238000010276 construction Methods 0.000 claims description 3
- 238000009826 distribution Methods 0.000 claims description 3
- 238000002955 isolation Methods 0.000 claims description 3
- 238000012423 maintenance Methods 0.000 claims description 3
- 239000000155 melt Substances 0.000 claims description 3
- 239000000203 mixture Substances 0.000 claims description 3
- 238000007789 sealing Methods 0.000 claims description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 3
- 238000010438 heat treatment Methods 0.000 abstract description 7
- 230000000903 blocking effect Effects 0.000 abstract 1
- 239000012295 chemical reaction liquid Substances 0.000 abstract 1
- 239000004743 Polypropylene Substances 0.000 description 4
- 238000006243 chemical reaction Methods 0.000 description 4
- -1 polypropylene Polymers 0.000 description 4
- 229920001155 polypropylene Polymers 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 3
- 238000010304 firing Methods 0.000 description 2
- 238000001746 injection moulding Methods 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000002360 explosive Substances 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 238000010030 laminating Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
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- Closures For Containers (AREA)
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Abstract
The invention discloses a microporous container and a manufacture method thereof. The microporous container comprises a foundation plate, microporous hollow cylinders, an upper cover plate and bottom films. The upper cover plate is arranged on the foundation plate. A plurality of blocking pipes extending into the microporous hollow cylinders respectively are arranged on the bottom of the upper cover plate, and the microporous hollow cylinders are arranged on the foundation plate in an arrayed mode. A following floatation block is arranged in each microporous hollow cylinder. The bottom films are arranged on the lower parts of the microporous hollow cylinders, and the parts, located on the bottoms of the microporous hollow cylinders, of the bottom films protrude towards the lower parts of the microporous hollow cylinders respectively. According to the invention, the bottom is thinner, the fitness is good, the heating speed is high, and heat conduction is more uniform; the following floatation blocks used for coverage are arranged in the microporous hollow cylinders, and therefore the phenomenon that reaction liquid is lost due to high temperature or the volatility can be reduced, and heat exchange caused by temperature difference between the upper cover plate and reactants can be reduced.
Description
Technical field
The present invention relates to micropore field of containers, particularly relate to a kind of micropore container and preparation method thereof.
Background technology
In PCR process, reagent copies multiplication to original DNA fragment by temperature cycles in each cycle.Each temperature cycle is made up of three steps usually: (1) sex change (~ 95 ° of C); (2) (~ 50 ° of C) is annealed; (3) expand/extend (~ 70 ° of C).There are two important factors to very crucial PCR detects: one is that the sample be seated in microwell plate can reach its temperature set-point fast can complete whole test in the short period of time; Two is for each temperature set-point, and reaction module keeps the uniformity of all micropore reaction temperatures of microwell array.
Patent of invention (patent No.: ZL201210091110.4) discloses a biological or chemical reaction method based on flat micropore container, although its flat micropore container can heat fast and uniformly, but due to Structure Designing Problem, the process more complicated of flat micropore vessel fabrication, particularly base material thickness is large, and poor with the laminating degree of heater, heat conduction is relatively slow, and inner shortage buoyant block, effectively cannot reduce the loss of reactant liquor.
Summary of the invention
The technical problem that the present invention mainly solves is to provide a kind of micropore container and preparation method thereof, obtains a kind of micropore container that can heat reactant liquor fast and uniformly, and reduces the loss of reactant liquor.
For solving the problems of the technologies described above, the technical scheme that the present invention adopts is: provide a kind of micropore container, comprise: base plate, microporous hollow cylinder, top cover plate and bottom surface film, described top cover plate is arranged on above base plate, several shutoff pipe extended to respectively in microporous hollow cylinder is provided with bottom the cover plate of described top, described microporous hollow cylinder difference array distribution is on base plate, a servo-actuated buoyant block is respectively arranged with in described microporous hollow cylinder, described bottom surface film is arranged on the below of microporous hollow cylinder, described bottom surface film is positioned at the below evagination of the position bottom microporous hollow cylinder respectively to microporous hollow cylinder.
In a preferred embodiment of the present invention, described base plate and microporous hollow cylinder are plastic cement integral structure, and described shutoff pipe and top cover plate are plastic cement integral structure, and the two ends of described microporous hollow cylinder give prominence to upper surface and the lower surface of base plate respectively.
In a preferred embodiment of the present invention, the outside diameter of described servo-actuated buoyant block is equal to or less than the internal diameter of microporous hollow cylinder, and the bottom of described servo-actuated buoyant block is provided with the arc surface of indent.
In a preferred embodiment of the present invention, the density of described servo-actuated buoyant block is less than water, and the surface of described servo-actuated buoyant block is provided with photothermal reflectance material.
In a preferred embodiment of the present invention, described bottom surface film is made up of thin film or plural layers compound.
In a preferred embodiment of the present invention, described bottom surface film is provided with photothermal reflectance layer, described photothermal reflectance layer is photothermal reflectance film or photothermal reflectance coating.
In a preferred embodiment of the present invention, described bottom surface film is whole the film covered bottom all microporous hollow cylinders.
In a preferred embodiment of the present invention, described bottom surface film comprises several sub-film of circle corresponding with bottom microporous hollow cylinder respectively, and the edge of the sub-film of described circle is connected with the bottom margin of microporous hollow cylinder respectively.
For solving the problems of the technologies described above, the technical scheme that the present invention adopts is: the preparation method providing a kind of micropore container, comprises the following steps:
Be shaped by plastic mould, obtain shutoff pipe and the top cover plate of integral structure;
Be shaped by plastic mould, obtain base plate and the microporous hollow cylinder of integral structure;
Be shaped by plastic mould, obtain servo-actuated buoyant block, be coated with one deck photothermal reflectance material on the surface of servo-actuated buoyant block;
Select plural layers compounds composition bottom surface film, in plural layers the material melting point of the film of top layer slightly lower than or the fusing point of equivalent microporous hollow cylinder material, in plural layers, the fusing point of the film of bottom is higher than the fusing point of microporous hollow cylinder material;
By melt process whole for bottom surface film sealing be fixed on all microporous hollow cylinders bottom or bottom surface film to be cut into after the sub-film of circle identical with size bottom single microporous hollow cylinder again sub-for circle film respectively hot-melting sealed in the bottom of microporous hollow cylinder;
In microporous hollow cylinder, inject reactant liquor, servo-actuated buoyant block is placed in microporous hollow cylinder respectively and swims on reactant liquor, then top cover plate is arranged on base plate, make shutoff pipe carry out shutoff to microporous hollow cylinder respectively.
In a preferred embodiment of the present invention, described melt process is: carry out preheating to high temperature flat board, make it the melting temperature of the film of top layer in a little higher than plural layers of surface temperature, but the melting temperature of the film lower than bottom, then utilize high temperature dull and stereotyped by the bottom surface of bottom surface thin film to microporous hollow cylinder, of short duration maintenance pressure 0.1 ~ 5 second, make the film melts of top layer and be combined with the bottom surface of microporous hollow cylinder, then high temperature plate isolation, achieve after room temperature is down in the bottom surface of bottom surface film and microporous hollow cylinder and be tightly connected, because the shrinkage factor difference of the bottom surface film of sandwich construction makes bottom surface film protrude outside microporous hollow cylinder after room temperature falls back in microporous hollow cylinder.
The invention has the beneficial effects as follows: a kind of micropore container that the present invention points out and preparation method thereof, utilize the slight evagination of bottom surface film, when being conducive to contacting with smooth temp controlled meter face bottom microporous hollow cylinder, can more be adjacent to this surface, to reach good thermo-contact, heat conduction is more even, more frivolous, thermal conductivity is good, and firing rate is fast, place the servo-actuated buoyant block covered in microporous hollow cylinder, be conducive to reducing reactant liquor because high temperature or its volatility run off.
Accompanying drawing explanation
In order to be illustrated more clearly in the technical scheme in the embodiment of the present invention, below the accompanying drawing used required in describing embodiment is briefly described, apparently, accompanying drawing in the following describes is only some embodiments of the present invention, for those of ordinary skill in the art, under the prerequisite not paying creative work, other accompanying drawing can also be obtained according to these accompanying drawings, wherein:
Fig. 1 is the structural representation of a kind of micropore container of the present invention an and preparation method thereof preferred embodiment;
Fig. 2 is the explosive view of Fig. 1;
Fig. 3 is the structural representation of a kind of micropore container of the present invention and preparation method thereof another preferred embodiment.
Detailed description of the invention
Be clearly and completely described to the technical scheme in the embodiment of the present invention below, obviously, described embodiment is only a part of embodiment of the present invention, instead of whole embodiments.Based on the embodiment in the present invention, those of ordinary skill in the art, not making other embodiments all obtained under creative work prerequisite, belong to the scope of protection of the invention.
Refer to Fig. 1 to Fig. 3, the embodiment of the present invention comprises:
A kind of micropore container, comprise: base plate 1, microporous hollow cylinder 2, top cover plate 3 and bottom surface film 6, described top cover plate 3 is arranged on above base plate 1, several shutoff pipe 4 extended to respectively in microporous hollow cylinder 2 is provided with bottom described top cover plate 3, described microporous hollow cylinder 2 difference array distribution is on base plate 1, a servo-actuated buoyant block 5 is respectively arranged with in described microporous hollow cylinder 2, described bottom surface film 6 is arranged on the below of microporous hollow cylinder 2, described bottom surface film 6 is positioned at the below evagination of the position bottom microporous hollow cylinder 2 respectively to microporous hollow cylinder 2.Bottom surface film 6 surface that is easier and heater when heating of evagination fits, and increases contact area, improves firing rate and uniformity.
Described base plate 1 is plastic cement integral structure with microporous hollow cylinder 2, described shutoff pipe 4 is plastic cement integral structure with top cover plate 3, the two ends of described microporous hollow cylinder 2 give prominence to upper surface and the lower surface of base plate 1 respectively, avoid the reactant liquor in microporous hollow cylinder 2 to splash on base plate 1.
The outside diameter of described servo-actuated buoyant block 5 is equal to or less than the internal diameter of microporous hollow cylinder 2, the bottom of described servo-actuated buoyant block 5 is provided with the arc surface of indent, reactant liquor is when heating, liquid easily volatilizees, servo-actuated buoyant block 5 is arranged on reactant liquor, and the arc surface of its indent effectively reduces the volatilization of reactant liquor.
The density of described servo-actuated buoyant block 5 is less than water, and the surface of described servo-actuated buoyant block 5 is provided with photothermal reflectance material, the light and heat of servo-actuated buoyant block 5 lower surface can be reflexed to reactant liquor inside, improves the efficiency of heating surface of reactant liquor, reduces thermal loss.
Described bottom surface film 6 is made up of thin film or plural layers compound.During plural layers, the fusing point of every layer is different, and the film melting points of lower floor is higher than the film melting points on its upper strata.
Described bottom surface film 6 is provided with photothermal reflectance layer, and described photothermal reflectance layer is photothermal reflectance film or photothermal reflectance coating.
Described bottom surface film 6 for covering whole film bottom all microporous hollow cylinders 2, as Fig. 3.The speed of whole film heat absorption and heat conduction is faster, but the pattern after bottom surface film 6 heat distortion is coarse, not as the sub-film of single circle comes attractive in appearance, or described bottom surface film 6 comprises several sub-film of circle corresponding with bottom microporous hollow cylinder 2 respectively, as Fig. 1 and Fig. 2, the edge of the sub-film of described circle is connected with the bottom margin of microporous hollow cylinder 2 respectively.
A preparation method for micropore container, comprises the following steps:
Be shaped by plastic mould, adopt polypropylene material injection moulding to obtain shutoff pipe 4 and the top cover plate 3 of integral structure;
Be shaped by plastic mould, adopt polypropylene material injection moulding to obtain base plate 1 and the microporous hollow cylinder 2 of integral structure;
Be shaped by plastic mould, polypropylene material is adopted to obtain servo-actuated buoyant block 5, servo-actuated buoyant block 5 adopts polypropylene material to make, density is less than reactant liquor, make servo-actuated buoyant block 5 be swum in the surface of reactant liquor, be coated with one deck photothermal reflectance material on the surface of servo-actuated buoyant block 5 to improve the efficiency of heating surface;
Select plural layers compounds composition bottom surface film 6, in plural layers the material melting point of the film of top layer slightly lower than or the fusing point of equivalent microporous hollow cylinder 2 material, in plural layers, the fusing point of the film of bottom is higher than the fusing point of microporous hollow cylinder 2 material;
By melt process whole for bottom surface film 6 sealing be fixed on all microporous hollow cylinders 2 bottom or bottom surface film to be cut into after the sub-film of circle identical with size bottom single microporous hollow cylinder 2 again sub-for circle film respectively hot-melting sealed in the bottom of microporous hollow cylinder 2;
In microporous hollow cylinder 2, inject reactant liquor, servo-actuated buoyant block 5 is placed in microporous hollow cylinder 2 respectively and swims on reactant liquor, then top cover plate 3 is arranged on base plate 1, make shutoff pipe 4 carry out shutoff to microporous hollow cylinder 2 respectively.
Described melt process is: carry out preheating to high temperature flat board, make it the melting temperature of the film of top layer in a little higher than plural layers of surface temperature, but the melting temperature of the film lower than bottom, then utilize high temperature dull and stereotyped by the bottom surface of bottom surface thin film to microporous hollow cylinder 2, of short duration maintenance pressure 0.1 ~ 5 second, make the film melts of top layer and be combined with the bottom surface of microporous hollow cylinder 2, then high temperature plate isolation, achieve after room temperature is down in the bottom surface of bottom surface film 6 and microporous hollow cylinder 2 and be tightly connected, because the shrinkage factor difference of the bottom surface film 6 of sandwich construction makes bottom surface film 6 protrude outside microporous hollow cylinder 2 after room temperature falls back in microporous hollow cylinder 2.
In sum, a kind of micropore container that the present invention points out and preparation method thereof, the heat-transfer rate block when micropore container obtained is heated, heating is more efficient, and heating uniformity is good, is conducive to the accuracy improving experimental data.
The foregoing is only embodiments of the invention; not thereby the scope of the claims of the present invention is limited; every utilize description of the present invention to do equivalent structure or equivalent flow process conversion; or be directly or indirectly used in other relevant technical field, be all in like manner included in scope of patent protection of the present invention.
Claims (10)
1. a micropore container, comprise: base plate, microporous hollow cylinder, top cover plate and bottom surface film, described top cover plate is arranged on above base plate, several shutoff pipe extended to respectively in microporous hollow cylinder is provided with bottom the cover plate of described top, it is characterized in that, described microporous hollow cylinder difference array distribution is on base plate, a servo-actuated buoyant block is respectively arranged with in described microporous hollow cylinder, described bottom surface film is arranged on the below of microporous hollow cylinder, and described bottom surface film is positioned at the below evagination of the position bottom microporous hollow cylinder respectively to microporous hollow cylinder.
2. micropore container according to claim 1, it is characterized in that, described base plate and microporous hollow cylinder are plastic cement integral structure, and described shutoff pipe and top cover plate are plastic cement integral structure, and the two ends of described microporous hollow cylinder give prominence to upper surface and the lower surface of base plate respectively.
3. micropore container according to claim 1, is characterized in that, the outside diameter of described servo-actuated buoyant block is equal to or less than the internal diameter of microporous hollow cylinder, and the bottom of described servo-actuated buoyant block is provided with the arc surface of indent.
4. micropore container according to claim 1, is characterized in that, the density of described servo-actuated buoyant block is less than water, and the surface of described servo-actuated buoyant block is provided with photothermal reflectance material.
5. micropore container according to claim 1, is characterized in that, described bottom surface film is made up of thin film or plural layers compound.
6. micropore container according to claim 1, is characterized in that, described bottom surface film is provided with photothermal reflectance layer, and described photothermal reflectance layer is photothermal reflectance film or photothermal reflectance coating.
7. micropore container according to claim 1, is characterized in that, described bottom surface film is whole the film covered bottom all microporous hollow cylinders.
8. micropore container according to claim 1, is characterized in that, described bottom surface film comprises several sub-film of circle corresponding with bottom microporous hollow cylinder respectively, and the edge of the sub-film of described circle is connected with the bottom margin of microporous hollow cylinder respectively.
9. a preparation method for the micropore container described in claim 1 ~ 8, is characterized in that, comprise the following steps:
Be shaped by plastic mould, obtain shutoff pipe and the top cover plate of integral structure;
Be shaped by plastic mould, obtain base plate and the microporous hollow cylinder of integral structure;
Be shaped by plastic mould, obtain servo-actuated buoyant block, be coated with one deck photothermal reflectance material on the surface of servo-actuated buoyant block;
Select plural layers compounds composition bottom surface film, in plural layers the material melting point of the film of top layer slightly lower than or the fusing point of equivalent microporous hollow cylinder material, in plural layers, the fusing point of the film of bottom is higher than the fusing point of microporous hollow cylinder material;
By melt process whole for bottom surface film sealing be fixed on all microporous hollow cylinders bottom or bottom surface film to be cut into after the sub-film of circle identical with size bottom single microporous hollow cylinder again sub-for circle film respectively hot-melting sealed in the bottom of microporous hollow cylinder;
In microporous hollow cylinder, inject reactant liquor, servo-actuated buoyant block is placed in microporous hollow cylinder respectively and swims on reactant liquor, then top cover plate is arranged on base plate, make shutoff pipe carry out shutoff to microporous hollow cylinder respectively.
10. the preparation method of micropore container according to claim 9, it is characterized in that, described melt process is: carry out preheating to high temperature flat board, make it the melting temperature of the film of top layer in a little higher than plural layers of surface temperature, but the melting temperature of the film lower than bottom, then utilize high temperature dull and stereotyped by the bottom surface of bottom surface thin film to microporous hollow cylinder, of short duration maintenance pressure 0.1 ~ 5 second, make the film melts of top layer and be combined with the bottom surface of microporous hollow cylinder, then high temperature plate isolation, achieve after room temperature is down in the bottom surface of bottom surface film and microporous hollow cylinder and be tightly connected, because the shrinkage factor difference of the bottom surface film of sandwich construction makes bottom surface film protrude outside microporous hollow cylinder after room temperature falls back in microporous hollow cylinder.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510486267.0A CN105128345B (en) | 2015-08-10 | 2015-08-10 | A kind of micropore container and preparation method thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510486267.0A CN105128345B (en) | 2015-08-10 | 2015-08-10 | A kind of micropore container and preparation method thereof |
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| Publication Number | Publication Date |
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| CN105128345A true CN105128345A (en) | 2015-12-09 |
| CN105128345B CN105128345B (en) | 2017-06-09 |
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| CN201510486267.0A Active CN105128345B (en) | 2015-08-10 | 2015-08-10 | A kind of micropore container and preparation method thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110218628A (en) * | 2019-06-19 | 2019-09-10 | 中国科学院半导体研究所 | A kind of digital pcr chip and preparation method thereof |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH03297378A (en) * | 1990-04-13 | 1991-12-27 | Seiko Instr Inc | Automatic reactor |
| US5459300A (en) * | 1993-03-03 | 1995-10-17 | Kasman; David H. | Microplate heater for providing uniform heating regardless of the geometry of the microplates |
| US20040086927A1 (en) * | 1990-11-29 | 2004-05-06 | Applera Corporation, A De Corporation | Thermal cycler for automatic performance of the polymerase chain reaction with close temperature control |
| US20040258568A1 (en) * | 1996-11-08 | 2004-12-23 | Eppendorf Ag | Thermostated block with heat-regulating devices |
| CN102899237A (en) * | 2011-04-28 | 2013-01-30 | 连彬 | Microplates, microplate modules and method for real-time monitoring temperature-controlled reaction on microplate |
| CN204874497U (en) * | 2015-08-10 | 2015-12-16 | 苏州晋翌生物医学仪器有限公司 | Micropore container |
-
2015
- 2015-08-10 CN CN201510486267.0A patent/CN105128345B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH03297378A (en) * | 1990-04-13 | 1991-12-27 | Seiko Instr Inc | Automatic reactor |
| US20040086927A1 (en) * | 1990-11-29 | 2004-05-06 | Applera Corporation, A De Corporation | Thermal cycler for automatic performance of the polymerase chain reaction with close temperature control |
| US5459300A (en) * | 1993-03-03 | 1995-10-17 | Kasman; David H. | Microplate heater for providing uniform heating regardless of the geometry of the microplates |
| US20040258568A1 (en) * | 1996-11-08 | 2004-12-23 | Eppendorf Ag | Thermostated block with heat-regulating devices |
| CN102899237A (en) * | 2011-04-28 | 2013-01-30 | 连彬 | Microplates, microplate modules and method for real-time monitoring temperature-controlled reaction on microplate |
| CN204874497U (en) * | 2015-08-10 | 2015-12-16 | 苏州晋翌生物医学仪器有限公司 | Micropore container |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110218628A (en) * | 2019-06-19 | 2019-09-10 | 中国科学院半导体研究所 | A kind of digital pcr chip and preparation method thereof |
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| CN105128345B (en) | 2017-06-09 |
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