CN105087677A - D-(-)-tartaric acid clean production process based on bipolar membrane electroosmosis technology - Google Patents
D-(-)-tartaric acid clean production process based on bipolar membrane electroosmosis technology Download PDFInfo
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Abstract
A purpose of the present invention is to provide a D-(-)-tartaric acid clean production process based on a bipolar membrane electroosmosis technology. The process comprises that: a cis-epoxy succinic acid sodium salt is hydrolyzed with D-type cis-epoxy succinic acid hydrolase (D-ESH) to obtain a D-(-)-sodium tartrate conversion solution; and the D-(-)-sodium tartrate contained in the D-(-)-sodium tartrate conversion solution is converted into D-(-)-tartaric acid through a bipolar membrane electordialyzer. According to the present invention, the current D-(-)-calcium tartrate acidification process can be replaced, the D-(-)-tartaric acid yield achieves more than or equal to 95%, and the additional acid is not required to provide H<+>; the generated NaOH solution can be used for synthesizing the precursor cis-epoxy sodium succinate, and the whole production process does not produce acid, alkali and salt waste solutions; and the production process has characteristics of environmental pollution reducing, raw material consumption reducing, significant industrial application values, and significant environmental benefits.
Description
Technical field
The present invention relates to a kind of D-(-)-tartrate process for cleanly preparing, produce the method being separated D-(-)-tartrate ion D-(-)-tartaric conversion fluid in particular to bipolar membrane electrodialysis device from enzymatic conversion method, belong to technical field of membrane.
Background technology
D-(-)-tartrate ((2S, 3S)-2,3-dihydroxyl butane-Isosorbide-5-Nitrae-dicarboxylic acid) is uncommon at occurring in nature, is applied in pharmaceutical industry mainly as the chiral source of chiral synthesize and chiral resolving agent.Along with industrial development, for D-(-)-tartaric demand also in cumulative year after year.
At present, industrially mainly adopt chemical resolution method for the production of D-(-)-tartaric method, racemic DL-tartrate is split as D-(-)-tartrate and L-(+)-tartrate (such as, see US Patent No. 6,346,, or utilize peculiar microorganism that L-(+)-tartrate in DL-tartrate is exhausted and obtained (such as, see EP0311835B) 649).These method operational difficultys and with high costs, limit D-(-)-tartaric output and application.
(-)-tartrate is energy conservation, eco-friendly method to utilize bio-transformation to produce D-, and existing multiple use bioconversion method produces D-(-)-tartaric report at present.Such as, Japanese Patent JP1996-245497 reports a kind of Pseudomonas (Pseudomonassp.), Japanese Patent JP1975-1445586 and JP2000-14391A report Alcaligenes (Alcaligenessp.) and document FEMSMicrobiolLett267,214(2007) report Bordetella 1-3 etc.Patent CN101338285A also reports a kind of Bordetella BK-52 and can be used for cis-form epoxy succinic acid or its salt to be converted into D-(-)-tartrate or its salt.
But, in the prior art, D-(-)-tartrate adopts calcium salt method to extract D-(-)-tartrate usually in producing, generally make it to generate D-(-)-calcium tartrate by adding calcium neutralizing agent in conversion fluid, through periodic crystallisation, clean after add sulfuric acid solution and extract D-(-)-tartrate, and just need can obtain consummate product by activated carbon decolorizing, ion-exchange after removing hetero-ion.Traditional calcium salt method can consume a large amount of sulfuric acid in process of production, and produce byproduct calcium sulfate, and Hydrolysis kinetics process steps is various, labour intensity and production cost high.
Bipolar membrane electrodialysis technology by use Bipolar Membrane, in the solution direct by water power from being H
+and OH
-, therefore, it is possible to the salt in the aqueous solution being converted into when not introducing new component corresponding bronsted lowry acids and bases bronsted lowry, being obtained in investigation and application two in recent years and developing rapidly.The domestic electrodialytic technique that not yet openly utilizes prepares D-(-)-tartaric report, CN102698603A reports a kind of tartaric production method, uses electrodialytic technique to extract L-(+)-tartrate from wine lees (main component is tartarus).But because the composition of natural winestone is L-(+)-tartarus, and D-(-)-tartrate is not present in nature in a large number, therefore this method be not suitable for D-(-)-tartaric production; On the other hand, in this process, although use potassium hydroxide that the tartarus in winestone is converted into the higher soluble tartrate of solubleness before extraction, but in leaching process, because anion-exchange membrane is different with the transparent performance of cationic exchange membrane to ion, be also easy to form the lower tartarus (under 25 DEG C of conditions, solubleness is only about 8g/L) of solubleness, and then causing film to block, this is unfavorable for industrial application; In order to avoid unfavorable factors such as film blockings, the concentration of solution unresolvable tartaric acid radical ion must remain on lower level, causes specific conductivity lower, need adopt conductive separator plate especially; And its production efficiency is also lower.
Summary of the invention
For the defect existed in prior art, the invention provides a kind of D-(-) based on bipolar membrane electrodialysis technology-tartrate process for cleanly preparing, thus advantageously enzymatic conversion method is produced tartrate and combine with bipolar membrane electrodialysis technology, D-(-)-tartrate is extracted efficiently from enzymatic conversion method liquid, simplify production process, realize the recycling of sodium hydroxide simultaneously.
The present invention is achieved through the following technical solutions:
Based on D-(-)-tartrate clean preparation method of bipolar membrane electrodialysis technology, mainly comprise the steps:
(1) use D-type cis-Epoxysuccinic acid hydratase (D-ESH) catalytic hydrolysis sodium hydrogen cis-epoxysuccinate, generate D-(-)-sodium tartrate conversion fluid;
(2) by bipolar membrane electrodialysis device, D-(-)-sodium tartrate contained in described D-(-)-sodium tartrate conversion fluid is converted into D-(-)-tartrate.
Preferably, the membrane cisterna of the electrodialyzer used in described step (2) is configured as: the salt that (1) is separated into anion-exchange membrane/sour two cell-types; Or (2) with acid/salt/alkali three-chamber type that anion-exchange membrane, cationic exchange membrane are separated into.
When salt/sour two cell-type, when producing, D-(-)-sodium tartrate conversion fluid is conveyed into salt room, D-(-)-tartaric acid solution is conveyed into sour room, material circulates under the effect of peristaltic pump, and adopts one or more in following working condition: current density is 80-140mA/cm
2, preferred 140mA/cm
2; Service temperature is 25-40 DEG C, preferably 40 DEG C; And the reaction times is 4-6h, preferred 6h.
When acid/salt/alkali three-chamber type, when producing, D-(-)-sodium tartrate conversion fluid is conveyed into salt room, D-(-)-tartaric acid solution is conveyed into sour room, NaOH solution is conveyed into alkali room, material circulates under the effect of peristaltic pump, and adopts one or more in following working condition: current density is 80-140mA/cm
2, preferred 80mA/cm
2; Service temperature is 25-40 DEG C, preferably 40 DEG C; And the reaction times is 4-6h, preferred 6h.
Can have multiple membrane cisterna in electrodialyzer of the present invention, described multiple membrane cisterna takes the arrangement mode of parallel connection or series connection.Preferably, can have 3 membrane cisternas be in series in electrodialyzer of the present invention, described membrane cisterna preferably can take salt/sour two cell-type configurations.
Preferably, in the present invention, before described step (1), described method also comprises the steps: that apparatus has the gene of sequence SEQIDNO:1 to encode, and obtains described D-type cis-Epoxysuccinic acid hydratase.
Technique of the present invention have employed bipolar membrane electrodialysis technology paratartaric acid and is separated, avoid production stage in prior art loaded down with trivial details, need to consume a large amount of sulfuric acid, inevitably produce the various defects such as byproduct, and achieve the recycle of sodium hydroxide in a preferred embodiment, whole technical process adopts enclosed circulation, has significant industrial application value and environmental benefit.
Accompanying drawing explanation
Fig. 1 is the schematic flow sheet of D-(-) of the present invention-tartrate production technique.
Fig. 2 is that salt of the present invention/sour two cell-type electrodialysis units prepare D-(-)-tartaric fundamental diagram, wherein, is followed successively by sour room and salt room from left to right.
Fig. 3 is that acid/salt of the present invention/alkali three-chamber type electrodialysis unit prepares D-(-)-tartaric fundamental diagram, wherein, is followed successively by sour room, salt room and alkali room from left to right.
Below the preferred embodiment of the present invention is described in detail.
Embodiment
Tartrate production technique of the present invention uses D-type cis-Epoxysuccinic acid hydratase (D-ESH) to be hydrolyzed to sodium hydrogen cis-epoxysuccinate, to obtain D-(-)-sodium tartrate.D-ESH is a kind of epoxide hydrolase (EpoxideHydrolases, EH), belongs to intracellular enzyme, can be added to by water molecules on cis-form epoxy succinic acid salt and form D-(-)-tartrate by Stereoselective.The use of producing D-ESH bacterium is known in the art, report that the bacterial classification for generation of D-ESH mainly comprises Pseudomonas alba (Pseudomonassp., JP1996-245497), Alcaligenes (Alcaligenes, JP1975-145586 and JP2000-14391A), Bordetella (Bordetella, FEMSMicrobiolLett267,214(2007) and CN101338285A) etc., by reference the full content of above document is incorporated to herein.
In a preferred embodiment, the gene fragment that D-ESH encodes can be connected on plasmid vector, and import E.coli cell, obtain recombinant bacterial strain, and use it for the present invention.Therefore, in a preferred embodiment, " D-type cis-Epoxysuccinic acid hydratase " used in the present invention also can refer to the above-mentioned recombinant bacterial strain used in reaction system.
Preferably, the present invention uses following gene fragment (SEQIDNO:1) to encode to D-type cis-Epoxysuccinic acid hydratase:
SEQIDNO：1
ATGACCCGTACCAAACTGATCCTGGAAGCTCGTATCAACGAATACATGCCGCGTCGTGGTAACCCGCACGTTCCGTGGACCCCGAAAGAAATCGGTGAAGCTGCTGCTCAGGCTCGTGAAGCTGGTGCTTCTATCGTTCACTTCCACGCTCGTCAGGCTGACGGTTCTCCGTCTCACGACTACGAAACCTACGCTGAATCTATCCGTGAAATCCGTGCTCGTTCTGACGTTCTGGTTCACCCGACCCTGGGTCAGATCACCCTGGGTGGTCGTGAATCTCGTCTGGCTCACATCGAACGTCTGTGCCTGGACCCGGCTCTGAAACCGGACTTCGCTCCGGTTGACCTGGGTTCTACCAACATCGACCGTTACGACGACGTTGAAAAACGTTACGAAACCGGTGACCGTGTTTACCTGAACAACATCGACACCCTGCAGCACTTCTCTAAACGTCTGCGTGAACTGGGTGTTAAACCGGCTTTCATCGCTTGGACCGTTCCGTTCACCCGTACCCTGGACGCTTTCATGGACATGGGTCTGGTTGACGACCCGGCTTACCTGCTGTTCGAACTGACCGACTGCGGTATCCGTGGTGGTCACCCGGGTACCATCCGTGGTCTGCGTGCTCACACCGACTTCCTGCCGCCGGGTCGTCAGATCCAGTGGACCGTTTGCAACAAAATCGGTAACCTGTTCGGTCCGGCTGCTGCTGCTATCGAAGAAGGTGGTCACGTTGCTATCGGTCTGGGTGACTACCTGTACCCGGAACTGGGTACCCCGACCAACGGTGAAGTTGTTCAGACCGTTGCTAACATGGCTCGTGCTATGGGTCGTGAAATCGCTACCCCGGCTGAAACCAAAGAAATCCTGGGTATCTCTAACTAA
Commercialization two cell-type in the market or three-chamber type bipolar membrane electrodialysis device all can be used for the present invention.Preferably, the electrodialyzer used in the present invention forms a Ge Mozu unit by dividing plate, Bipolar Membrane, cationic exchange membrane and anion-exchange membrane according to the membrane cisterna configuration of setting, one or several module unit is assembled between pair of electrodes, forms bipolar membrane electrodialysis device.Membrane cisterna is configured as salt/sour two Room (as shown in Figure 2) of being separated into anion-exchange membrane or the acid/salt/alkali three-chamber type (as shown in Figure 3) for being separated into anion-exchange membrane, cationic exchange membrane.
In salt of the present invention/sour two cell-type membrane cisternas, between two Bipolar Membrane, be separated into sour room and salt room with anion-exchange membrane.Conversion fluid enters the salt room on the right, and under DC electric field effect, negatively charged ion D-(-)-tartrate anion enters acid room, the left side by anion-exchange membrane, forms D-(-)-tartrate; And the positively charged ion (Na in salt room
+) OH that produces with Bipolar Membrane
-form alkali.This electroosmose process can obtain the mixture of pure D-(-)-tartrate and salt and alkali.
In acid/salt of the present invention/alkali three-chamber type membrane cisterna, between two Bipolar Membrane, separate the sour room of composition, salt room and alkali room with anion-exchange membrane and cationic exchange membrane.After conversion fluid enters middle salt room, under DC electric field effect, positively charged ion (Na
+) enter alkali room by cationic exchange membrane, the OH produced with Bipolar Membrane
-form alkali; Negatively charged ion D-(-)-tartrate anion then enters sour room by anion-exchange membrane, the H produced with Bipolar Membrane
+form D-(-)-tartrate.
Preferably, the module unit in the electrodialyzer used in the present invention can take the mode of parallel connection or series connection to carry out.
Preferably, the operating parameters of the electrodialyzer in the present invention is: during production, D-(-)-sodium tartrate conversion fluid is conveyed into salt room, D-(-)-tartaric acid solution is conveyed into sour room (two cell-types and three-chamber type), NaOH solution is conveyed into alkali room (three-chamber type), material circulates under the effect of peristaltic pump, and current density is 80-140mA/cm
2, service temperature is 25-40 DEG C, and the reaction times is 4-6h.
Below in conjunction with embodiment, present invention is described.
Embodiment
In following examples 1, the synthetic method of substrate sodium hydrogen cis-epoxysuccinate is: added by 300mL deionized water in 1000mL flask, under agitation add MALEIC ANHYDRIDE 120g, after it dissolves, slowly add the NaOH solution prepared by 50g sheet alkali, 180mL pure water, temperature of reaction rises to 55 DEG C gradually.Then 7g sodium wolframate is added in flask, then by 162mL27.5%H
2o
2in instillation flask, temperature in process, is kept to be 60 DEG C.H
2o
2after dripping, insulation reaction 2h.After reaction terminates, regulate about pH to 8.5 with NaOH, stand-by.
Other reagent of using is synthesized (as MALEIC ANHYDRIDE, NaOH, sodium wolframate, H for substrate
2o
2deng) be domestic analytical pure.
In following examples 2-4, the model of the bipolar membrane electrodialysis device used is CJ-BMED-A, and produced by Hefei Ke Jia macromolecular material Science and Technology Ltd., overall dimensions are 650 × 650 × 950mm, membrane stack is of a size of 170mm × 110mm, and ion-exchange membrane is acidproof alkaline.The preparation of embodiment 1D-type cis-Epoxysuccinic acid hydratase and enzymically hydrolyse thereof
The gene fragment of SEQIDNO:1 is connected on pBV220 plasmid vector, then recombinant plasmid is imported in E.coliDH5 α competent cell, obtain restructuring D-type cis-Epoxysuccinic acid hydratase engineering bacteria.The culture condition of engineering bacteria is: in 500mL triangular flask, load 100mLLB substratum, picking engineering bacteria list bacterium colony access substratum, be placed in 37 DEG C, shaking culture under 200rpm condition, as OD600=0.6-0.8, terminate after temperature being risen to 42 DEG C of inducing culture 5h, 8000g collected by centrifugation somatic cells, stand-by.
Above-mentioned 0.4gE.coli somatic cells is added in the sodium hydrogen cis-epoxysuccinate solution (pH8.0) of 500mL1.2M, in 200rpm, carry out catalyzed reaction under 37 DEG C of conditions, stopped reaction after about 10h, obtain conversion fluid, the transformation efficiency of sodium hydrogen cis-epoxysuccinate can reach more than 98%.
Embodiment 2 salt/sour two cell-type electrodialysis units prepare D-(-)-tartrate
2L is pumped into the salt room (Fig. 2) of salt/sour two cell-type electrodialyzers by the conversion fluid obtained in embodiment 1 with the speed of 60L/h, with the speed of 40L/h, D-(-)-tartaric acid solution of 0.5L0.2M is pumped into sour room to increase its electroconductibility simultaneously.Control voltage makes current density maintain 140mA/cm
2, service temperature is 40 DEG C, and the transformation efficiency of circulating reaction 6h, D-(-)-tartrate anion reaches 86%, and energy consumption is 2.09kWh/kg, and current efficiency is that about 88%, D-(-)-tartrate rate of recovery reaches 95%.
Embodiment 3 acid/salt/alkali three-chamber type electrodialysis unit prepares D-(-)-tartrate
2L is pumped into the salt room (Fig. 3) of acid/salt/alkali three-chamber type electrodialyzer with the speed of 80L/h by the conversion fluid obtained in embodiment 1, with the speed of 60L/h, D-(-)-tartaric acid solution of 1L0.2M is pumped into sour room simultaneously, with the speed of 60L/h, the NaOH solution of 1L0.1M is pumped into alkali room, to increase its electroconductibility.Control voltage makes current density maintain 80mA/cm
2, service temperature is 40 DEG C, and the transformation efficiency of circulating reaction 6h, D-(-)-tartrate anion reaches 90%, and energy consumption is 2.64kWh/kg, and current efficiency is that about 90%, D-(-)-tartrate rate of recovery reaches 98%.
Embodiment 4 two cell-type module unit tandem type electrodialysis unit prepares D-(-)-tartrate
To connect between the pair of electrodes of electrodialyzer the module unit shown in 3 picture groups 2, form anolyte compartment-sour room-salt room-sour room-salt room-sour room-multiple compartment of salt room-cathode compartment.D-(-)-tartaric acid solution of 0.2M is all poured in acid room, and flow velocity is 80L/h, to increase the electroconductibility of sour room.The conversion fluid (obtaining by embodiment 1) containing 1.2MD-(-)-tartrate ion is all poured in salt room, and flow velocity is 100L/h.Control voltage makes current density maintain 140mA/cm
2, service temperature is 30 DEG C, and the transformation efficiency of reaction 4h, D-(-)-tartrate anion reaches 92%, and energy consumption is 3.23kWh/kg, and current efficiency is that about 92%, D-(-)-tartrate rate of recovery reaches 98%.
Claims (9)
1. D-(-)-tartrate production method, comprises the steps:
(1) use D-type cis-Epoxysuccinic acid hydratase catalytic hydrolysis sodium hydrogen cis-epoxysuccinate, generate D-(-)-sodium tartrate conversion fluid;
(2) by bipolar membrane electrodialysis device, D-(-)-sodium tartrate contained in described D-(-)-sodium tartrate conversion fluid is converted into D-(-)-tartrate.
2. the method for claim 1, is characterized in that, the membrane cisterna of the electrodialyzer used in described step (2) is configured as salt/sour two cell-types be separated into anion-exchange membrane.
3. the method for claim 1, is characterized in that, the membrane cisterna of the electrodialyzer used in described step (2) is configured as the acid/salt/alkali three-chamber type be separated into anion-exchange membrane, cationic exchange membrane.
4. method as claimed in claim 2 or claim 3, it is characterized in that having multiple membrane cisterna in described electrodialyzer, described multiple membrane cisterna takes the arrangement mode of parallel connection or series connection.
5. method as claimed in claim 2, it is characterized in that, when producing, described D-(-)-sodium tartrate conversion fluid is conveyed into salt room, D-(-)-tartaric acid solution is conveyed into sour room, material circulates under the effect of peristaltic pump, and adopts one or more in following working condition:
Current density is 80-140mA/cm
2, preferred 140mA/cm
2;
Service temperature is 25-40 DEG C, preferably 40 DEG C; And
Reaction times is 4-6h, preferred 6h.
6. method as claimed in claim 3, it is characterized in that, when producing, described D-(-)-sodium tartrate conversion fluid is conveyed into salt room, D-(-)-tartaric acid solution is conveyed into sour room, NaOH solution is conveyed into alkali room, material circulates under the effect of peristaltic pump, and adopts one or more in following working condition:
Current density is 80-140mA/cm
2, preferred 80mA/cm
2;
Service temperature is 25-40 DEG C, preferably 40 DEG C; And
Reaction times is 4-6h, preferred 6h.
7. method as claimed in claim 6, is characterized in that, the sodium hydroxide solution produced is reused for the production of sodium hydrogen cis-epoxysuccinate in alkali room.
8. method as claimed in claim 4, it is characterized in that, described electrodialyzer has two cell-type membrane cisternas of three groups of series connection, when producing, described D-(-)-sodium tartrate conversion fluid is conveyed into the salt room of each membrane cisterna, D-(-)-tartaric acid solution is conveyed into sour room, and material circulates under the effect of peristaltic pump, and adopts one or more in following working condition:
Current density is 80-140mA/cm
2, preferred 140mA/cm
2;
Service temperature is 25-40 DEG C, preferably 30 DEG C; And
Reaction times is 4-6h, preferred 4h.
9. the method according to any one of claim 1-8, is characterized in that, before described step (1), described method also comprises the steps: that apparatus has the gene of sequence SEQIDNO:1 to encode, and obtains described D-type cis-Epoxysuccinic acid hydratase.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108084064A (en) * | 2017-12-22 | 2018-05-29 | 浙江金伯士药业有限公司 | A kind of new preparation process of D- (-)-tartaric acid |
| CN108299217A (en) * | 2017-01-13 | 2018-07-20 | 南京红杉生物科技有限公司 | A kind of process and system producing D-phenylalanine |
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